CN113966704A - Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety - Google Patents
Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety Download PDFInfo
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- CN113966704A CN113966704A CN202111255931.2A CN202111255931A CN113966704A CN 113966704 A CN113966704 A CN 113966704A CN 202111255931 A CN202111255931 A CN 202111255931A CN 113966704 A CN113966704 A CN 113966704A
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- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 124
- 241000208125 Nicotiana Species 0.000 title claims abstract description 94
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000006698 induction Effects 0.000 title claims description 15
- 239000002689 soil Substances 0.000 claims abstract description 42
- 244000061176 Nicotiana tabacum Species 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 230000001939 inductive effect Effects 0.000 claims abstract description 23
- 201000010099 disease Diseases 0.000 claims abstract description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 12
- 230000001502 supplementing effect Effects 0.000 claims abstract description 7
- 230000003020 moisturizing effect Effects 0.000 claims 1
- 230000008506 pathogenesis Effects 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 10
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 241000233629 Phytophthora parasitica Species 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 239000013589 supplement Substances 0.000 description 4
- 238000002054 transplantation Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 2
- 244000020518 Carthamus tinctorius Species 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 241001522633 Betula utilis subsp. albosinensis Species 0.000 description 1
- 239000008830 Carthamus tinctorius Honghua extract Substances 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The application discloses a black shank induced morbidity method for potted tobacco leaves of Honghuadajinyuan variety, which comprises the following steps: 1) before tobacco seedling transplanting, selecting soil which is 10-20 cm below the soil moisture surface in the field with black shank in the past year as an induced soil sample; 2) when the tobacco seedlings are transplanted, the inducing soil sample is placed at the base of the shin of each tobacco seedling, and not less than 50g of inducing soil sample is applied to each tobacco seedling; 3) after the experimental tobacco seedlings are transplanted for 7-10 days, supplementing water to the experimental tobacco seedlings; the field with high incidence of black shank refers to: the number of the tobacco plants with black shank disease accounts for more than 90 percent of the total amount of the tobacco plants planted in the field. The method is beneficial to the growth and survival of tobacco seedlings and improves the reliability of the experiment, and the incidence rate of black shank of the tobacco seedlings is over 70 percent by adopting the method to experiment.
Description
Technical Field
The application relates to the technical field of tobacco leaf black shank experiment simulation, in particular to a black shank induction morbidity method for potted tobacco leaves of a Honghua Dajinyuan variety.
Background
Honghuadajinyuan (abbreviated as "Hongda") is a representative of special tobacco leaves and is favored by cigarette enterprises. In recent years, the planting proportion of Honghuadajinyuan in tobacco regions in China is stabilized at about 4%, and the planting area in southwest regions such as Yuanduichuan is relatively large. But the difficulty of the variety in inducing black shank is high.
In the process of developing a black shank medicament control test, in order to better and more accurately observe the control effect of various medicaments, tobacco plants are induced to be diseased and used as test materials, and after the test tobacco plants are applied, medicaments with the best control effect are screened out according to the disease-resistant effect of the tobacco plants.
For this reason, the tobacco plants to be tested are subjected to an induced disease operation before the start of the drug test.
The existing induced morbidity method comprises the following steps: selecting tobacco plants with black shank from the field, separating black shank strains from the tobacco plants, then culturing and propagating the germ plants in a laboratory, and transplanting tobacco leaves after the propagation work is finished. When transplanting, the propagated strains are inoculated to the base part of the shin of each tobacco and then soil-covered for transplanting.
The following disadvantages exist with the above method:
1. the transplanting period of the tobacco seedlings is limited by the strain propagation work, the tobacco seedling transplanting can be carried out only after the propagation of the black shank strain is completed, and if the strain propagation work cannot be completed in time, the experimental tobacco seedlings cannot be transplanted within the optimal control. Affecting the growth and survival rate of the tobacco seedlings in the experiment.
2. Although the method can be used for directionally and accurately inoculating the phytophthora parasitica to the experimental tobacco seedlings, the induced morbidity of the tobacco seedlings after inoculation is low and is only between 40 and 60 percent.
Disclosure of Invention
The application provides a phytophthora parasitica induction morbidity method for potted tobacco leaves of a Honghuadajinyuan variety, which is used for solving the problems that in the prior art, the phytophthora parasitica propagation progress interferes with the transplantation opportunity of experimental tobacco seedlings by adopting the induction morbidity method of inoculating the phytophthora parasitica after propagation; the tobacco seedling induction disease incidence is low.
The application provides a black shank induced morbidity method for potted tobacco leaves of Honghuadajinyuan variety, which comprises the following steps:
1) before tobacco seedling transplanting, selecting soil which is 10-20 cm below the soil moisture surface in the field with black shank in the past year as an induced soil sample;
2) when the tobacco seedlings are transplanted, the inducing soil sample is placed at the base of the shin of each tobacco seedling, and not less than 50g of inducing soil sample is applied to each tobacco seedling;
3) after the experimental tobacco seedlings are transplanted for 7-10 days, supplementing water to the experimental tobacco seedlings at 2-3 points every afternoon;
the field with high incidence of black shank refers to: the number of the tobacco plants with black shank disease accounts for more than 90 percent of the total amount of the tobacco plants planted in the field.
By adopting the method, the black shank germs in the soil with high incidence of the black shank can be effectively utilized, the effective directional induction of the experimental tobacco seedling is realized, and the induced soil is selected according to the method, so that the infection of other mixed germs on the experimental tobacco seedling can be avoided, and the normal operation of the experiment is influenced. Meanwhile, the soil-borne characteristic of the black shank is utilized, and the induction soil is adopted for induction, so that the infection proportion of the tobacco strain after induction can be effectively increased to 70 percent, which is higher than that of the existing common strain inoculation induction method. The method does not need to perform germ culture, propagation and inoculation, is simple to operate, is suitable for rapidly inducing black shank in large batch, has low requirements on experimental environment, and is suitable for rapidly carrying out experiments on experimental sites lacking sterile environment. The tobacco seedlings are supplemented with water in the time period, so that the incidence of black shank infected by tobacco seedling plants can be promoted, and the incidence proportion is improved.
By adopting the method, the black shank of the experimental tobacco seedling can be effectively induced on the premise of meeting the requirement of the experimental tobacco seedling transplanting festivals, and the problem that the tobacco seedling transplanting is not festivals or cannot induce the black shank is solved.
The soil with the depth is selected as the induced soil, so that the induced soil can be effectively prevented from being polluted by other mixed bacteria.
Preferably, the water replenishing operation: the water supplementing quantity of each experimental tobacco seedling is not less than 500 ml. According to the water supplementing quantity, the survival rate of the transplanted experimental tobacco seedlings can be effectively improved, and the normal surprise of the experiment is facilitated.
Preferably, the water replenishing operation: each experimental tobacco seedling is continuously replenished with water for 7-10 days. According to the period, water is supplemented, and the normal growth of the plants can be effectively ensured.
Preferably, the water replenishing operation is performed at 2 pm per day. The tobacco seedlings are supplemented with water in the time period, and the disease incidence proportion is the largest.
Preferably, the tobacco plants are continuously planted in the past year for 2 years, and the number of the black shank ill tobacco plants in each year accounts for more than 90% of the total planting amount of the field. The induced soil adopting the condition can meet the requirement on the bacterium content of the black shank bacterium, avoids the interference and influence of the mixed bacterium on the experiment, can only induce to obtain the black shank infected tobacco plant, and can not generate other germ tobacco plants simultaneously.
Preferably, the induced soil sample is applied to wrap around the base of the shank of the experimental tobacco seedling. By adopting the operation, the induced soil can be ensured to be effectively contacted with the diseased part of the tobacco plant, and the disease proportion is favorably improved.
The beneficial effects that this application can produce include:
1) the induction method for black shank induction morbidity of the potted tobacco leaves of the Honghuadajinyuan variety is simple to operate, does not need culture, propagation and inoculation operations of black shank bacteria in an aseptic environment, and can be used for carrying out experiment operation in any tobacco area; the induced soil is filled at the base part of the shin when the experimental tobacco seedlings are transplanted, the tobacco seedling transplantation can be carried out according to the control, the growth and survival of the tobacco seedlings are facilitated, the experimental reliability is improved, and the incidence rate of black shank disease of the experimental tobacco seedlings by adopting the method is over 70 percent.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the following detailed description will be given with reference to specific experimental results in the embodiments of the present invention.
Examples
The tobacco seedlings and other laboratory instruments used in the following examples were obtained commercially without specific reference.
Example 1
The method for inducing and attacking the black shank of the potted tobacco leaves of the Honghuadajinyuan variety comprises the following steps of:
1) before tobacco seedling transplanting, selecting soil which is 10cm below the soil moisture surface in the field with black shank in the past year as an induced soil sample;
2) selecting 100 safflower Honghuadajinyuan varieties which grow for 70 days after sowing as experimental tobacco seedlings, and placing the inducing soil samples at the base of the shin of each tobacco seedling when the tobacco seedlings are transplanted, wherein the amount of the inducing soil samples applied to each tobacco seedling is not less than 50 g; the field with high incidence of black shank refers to: the number of the tobacco plants with black shank disease accounts for more than 90 percent of the total amount of the tobacco plants planted in the field.
3) After 7 days of experimental tobacco seedling transplantation, the tobacco plants are supplemented with water at 2 pm every day, the water supplement amount of each plant is not less than 500ml every time, and the water supplement is continuously carried out for 7 days.
Example 2
The method for inducing and attacking the black shank of the potted tobacco leaves of the Honghuadajinyuan variety comprises the following steps of:
1) before tobacco seedling transplanting, selecting soil 20cm below the soil moisture surface in the field with black shank in the past as an induced soil sample;
2) selecting 100 safflower Honghuadajinyuan varieties which grow for 65 days after sowing as experimental tobacco seedlings, and placing the inducing soil samples at the base of the shin of each tobacco seedling when the tobacco seedlings are transplanted, wherein the amount of the inducing soil samples applied to each tobacco seedling is not less than 50 g; the field with high incidence of black shank refers to: the number of the tobacco plants with black shank disease accounts for more than 90 percent of the total amount of the tobacco plants planted in the field.
3) After the experimental tobacco seedlings are transplanted for 10 days, the tobacco plants are supplemented with water at 3 pm every day, the water supplement is not less than 500ml for each plant every time, and the water supplement is continuously carried out for 10 days.
Example 3
The method for inducing and attacking the black shank of the potted tobacco leaves of the Honghuadajinyuan variety comprises the following steps of:
1) before tobacco seedling transplanting, selecting soil 15cm below the soil moisture surface in the field with black shank in the past as an induced soil sample;
2) selecting 100 plants of the Honghuadajinyuan variety which grows for 68 days after sowing as experimental tobacco seedlings, and placing the inducing soil sample at the base of the shin of each tobacco seedling when the tobacco seedlings are transplanted, wherein the amount of the inducing soil sample applied to each plant is not less than 50 g; the field with high incidence of black shank refers to: the number of the tobacco plants with black shank disease accounts for more than 90 percent of the total amount of the tobacco plants planted in the field.
3) After 8 days of experimental tobacco seedling transplantation, 2% in the afternoon every day: and (5) replenishing water to the tobacco plants at 30 points, wherein the water is not less than 500ml per plant every time, and continuously replenishing water for 8 days.
Comparative example 1
The difference from example 1 is that: selecting tobacco plants with black shank from the field, separating black shank strains from the tobacco plants, then culturing and propagating the germ plants in a laboratory, and transplanting tobacco leaves after the propagation work is finished. When transplanting, the propagated strains are inoculated to the base part of the shin of each of 100 experimental tobacco seedlings, and then soil is covered for transplanting. The operations not described in detail above are performed according to the microbial inoculation operation, and will not be described in detail herein.
The black shank induction results of examples 1 to 3 and comparative example 1 on tobacco seedlings are shown in table 1:
TABLE 1
| Item | Incidence of blackleg (%) | Survival rate of tobacco seedling (%) |
| Example 1 | 81 | 100 |
| Example 2 | 70 | 100 |
| Example 3 | 79 | 100 |
| Comparative example 1 | 45％ | 60 |
As can be seen from Table 1, when the method provided by the application is adopted to induce tobacco seedlings, the incidence rate of black shank can be effectively increased, and the normal operation of experiments is facilitated. Although the existing method for inoculating the germs can avoid other mixed germs from polluting an experimental sample, the induced infection rate is low, and the growth of tobacco seedlings is influenced.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (6)
1. A method for inducing and attacking black shank of potted tobacco leaves of Honghuadajinyuan variety is characterized by comprising the following steps:
1) before tobacco seedling transplanting, selecting soil which is 10-20 cm below the soil moisture surface in the field with black shank in the past year as an induced soil sample;
2) when the tobacco seedlings are transplanted, the inducing soil sample is placed at the base of the shin of each tobacco seedling, and not less than 50g of inducing soil sample is applied to each tobacco seedling;
3) after the experimental tobacco seedlings are transplanted for 7-10 days, supplementing water to the experimental tobacco seedlings at 2-3 points every afternoon;
the field with high incidence of black shank refers to: the number of the tobacco plants with black shank disease accounts for more than 90 percent of the total amount of the tobacco plants planted in the field.
2. The method for inducing and attacking blackleg of potted tobacco leaves of Honghuadajinyuan variety according to claim 1, wherein the water replenishing operation comprises: the water supplementing quantity of each experimental tobacco seedling is not less than 500 ml.
3. The method for black shank induction pathogenesis of potted tobacco leaf of Honghuadajinyuan variety according to claim 1, wherein said moisturizing is performed at 2 pm per day.
4. The method for inducing and attacking blackleg of potted tobacco leaves of Honghuadajinyuan variety according to claim 1, wherein the water replenishing operation comprises: the water supplementing quantity of each experimental tobacco seedling is not less than 500 ml.
5. The method for inducing and attacking black shank of potted tobacco leaves of Honghuadajinyuan variety according to claim 1, wherein the last year is 2 years of continuous planting of tobacco plants, and the number of black shank attacked tobacco plants per year accounts for more than 90% of the total planting amount of the field.
6. The method for inducing onset of blackleg of potted tobacco leaves of the Honghuadajinyuan variety according to claim 1, wherein an inducing soil sample is applied to surround the base of the shin of the test tobacco seedling.
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| CN202111255931.2A CN113966704A (en) | 2021-10-27 | 2021-10-27 | Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety |
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| CN202111255931.2A CN113966704A (en) | 2021-10-27 | 2021-10-27 | Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114847114A (en) * | 2022-04-18 | 2022-08-05 | 河南农业大学 | Application of trichoderma harzianum application mode to tobacco growth and induced resistance |
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- 2021-10-27 CN CN202111255931.2A patent/CN113966704A/en active Pending
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Cited By (1)
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