CN103703986A - Cultivating method for solanaceous plants - Google Patents
Cultivating method for solanaceous plants Download PDFInfo
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- CN103703986A CN103703986A CN201310728136.XA CN201310728136A CN103703986A CN 103703986 A CN103703986 A CN 103703986A CN 201310728136 A CN201310728136 A CN 201310728136A CN 103703986 A CN103703986 A CN 103703986A
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Abstract
The invention provides a cultivating method for solanaceous plants. The method comprises the following steps of implanting rooted seedlings obtained through seeds or tissue culture into solid substrates, inoculating Glomus mosseae to the substrates, inoculating pseudomonadaceae to the substrates after the plantules of seeds germinate and are exposed out of the substrates to form seedlings, inoculating pseudomonadaceae to the substrates while the rooted seedlings are implanted to the substrates and when the rooted seedlings obtained through the tissue culture are cultivated, obtaining the seedlings with the fungi after the pseudomonadaceae is inoculated, and cultivating the seedlings to ripe. According to the method, in the cultivating process of the solanaceous plants, the Glomus mosseae and the pseudomonadaceae are inoculated to the seedlings successively, the cultivated solanaceous plants are made to show excellent drought resistance, frost resistance, disease resistance and other resistance performance in a seedling stage, and meanwhile, the grow rate of the seedlings of the solanaceous plants is improved greatly under the synergistic effects of the two symbiotic fungi.
Description
Technical field
The present invention relates to a kind of plant cultivation method, be specifically related to a kind of plant of Solanaceae breeding method.
Background technology
Plant of Solanaceae comprises the crops such as capsicum, eggplant, tomato, tobacco, is human lives's Common Vegetables.Plant of Solanaceae is dicotyledon, its seed endosperm content is lower, cannot be for the tissues such as plumule provide enough nutrient components when sprouting, cause plant of Solanaceae by seminal propagation seedling stage vitality extremely a little less than, in production, show as the multiple damage by disease and insect such as damping off, damping off and cabbage caterpillar, cutworm, chafer very easily occur.During because of soil nutrient imbalance, also easily occur nutritional deficiency disease, thin and weak seedling being damaged to plants caused by sudden drop in temperature freeze injury because nursery stage temperature instability causes seedling, often to agricultural production, cause heavy losses.
Existing solution is by using chemical pesticide control damage by disease and insect, solving nutritional deficiency by fertilising, by epiphragma, the solution such as strengthen management damage to plants caused by sudden drop in temperature, wet injury.Above-mentioned way complex steps, if unreasonable fertilizer damage or the nutritional imbalance of often can producing that apply fertilizer causes effect bad, quality of vegetable declines, and also can produce sub-pin acid pollution environment and underground water; Chemical pesticide is used and improperly also can be caused the pesticide-tainted vegetable events such as residue of pesticide.Therefore, now studies have reported that and can strengthen the adaptive capacity of seedling to environment to its seedling inoculation symbiotic microorganism when plant of Solanaceae is grown seedlings, improve its survival rate.But when practical application, above-mentioned fungal component seedling method does not show outstanding effect.
Summary of the invention
In view of this, the present invention discloses a kind of breeding method that strengthens plant of Solanaceae resistance.
Object of the present invention is achieved through the following technical solutions:
A plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Described Glomus mosseae can be commercially available Glomus mosseae microbial inoculum, and described pseudomonad can be any pseudomonad.Glomus mosseae is a kind of common fungi that parasitizes plant of Solanaceae, is generally used for and improves soil property, also has report to utilize Glomus mosseae to strengthen the ability of plant of Solanaceae resistance to bacterial wilt.But not yet there is the technology of utilizing Glomus mosseae to strengthen plant of Solanaceae Seedling Stage resistance, and separately to seedling inoculation Glomus mosseae, also do not make seedling give expression to higher than not inoculating seedling resistance, the characteristic of especially anti-nutritional deficiency disease, freeze proof, drought resisting.Even if the disease-resistant characteristics such as Glomus mosseae resistance to bacterial wilt on the books, when its seedling is planted on the field of plant of Solanaceae continuous cropping, its effect is also very little.Pseudomonad is a kind of Gram-negative bacteria, and it is considered to the cause of disease of plant of Solanaceae bacterial wilt conventionally its subordinate's Pseudomonas solanacearum, and generally not recording it has facilitation to the growth of plant of Solanaceae seedling.And the first backward seed and seedling inoculation Glomus mosseae of the present invention and pseudomonad, but make cultivated plant of Solanaceae show the adverse-resistant characteristics such as good drought resisting, freeze proof, disease resistance at Seedling Stage, simultaneously under the synergy of two kinds of fungal components, described plant of Solanaceae growth of seedling speed also has larger lifting, after conjecture is inoculating two kinds fungal component, plant of Solanaceae is improved to the absorption of nutrient ingredients ability in soil.In advance the taking root seedling of seed or group training acquisition is inoculated after Glomus mosseae, the seedling inoculation pseudomonad becoming to seed germination again, seedling is improved a lot to the tolerance of the diseases such as bacterial wilt, after conjecture is the seedling symbiosis of Glomus mosseae and plant of Solanaceae, its thalline or its metabolite can with pseudomonad generation antagonism, make the pathogenic bacteria of infecting seedling lose pathogenecity.Described step c carries out conventional cultivation by seedling replanting in soil, arable land.Especially, in the present invention, inoculated the plant of Solanaceae seedling of Glomus mosseae and pseudomonad, even if it is by the soil of cultivation and plant of Solanaceae continuous cropping, the pathogenic flora that is also difficult for being accumulated in soil infects simultaneously.This effect is also reported there are no related art.In addition, because pseudomonad PI human body makes it ill, for preventing that the use parts such as the final fruit of gathering in the crops, plant corpus from remaining pathogenic pseudomonad, the present invention, especially only to matrix inoculation pseudomonad, also should avoid making pseudomonad inoculation seedling to expose the part of matrix as far as possible during inoculation.The alleged pseudomonad of the present invention, should get rid of the Pseudomonas solanacearum that can make plant of Solanaceae suffer from bacterial wilt.The described seedling of taking root can be plant of Solanaceae that any tissue culture method the obtains seedling of taking root.Described step c can take existingly plant of Solanaceae seedling is cultured to ripe method carries out.
Further, also comprise after inoculation pseudomonad, to matrix and seedling, add polyunsaturated fatty acid.
Through inventor's checking, after inoculation pseudomonad, to matrix and seedling, use after polyunsaturated fatty acid, can stimulate significantly growth of seedling, improve the growth rate of seedling.Meanwhile, the adverse-resistant characteristic such as polyunsaturated fatty acid, disease resistance freeze proof to seedling also has larger facilitation.Infer that polyunsaturated fatty acid can promote symbiosis in plant of Solanaceae tissue or the microorganism species of rhizosphere is grown, thereby provide more sufficient useful metabolite or nutriment for plant of Solanaceae.
Further, described polyunsaturated fatty acid is the straight chain fatty acid of carbon chain lengths 18-22, and its unsaturated bond quantity is 2-6.
Through inventor's checking, the carbon chain lengths of polyunsaturated fatty acid, degree of unsaturation have a certain impact to the effect of above-mentioned promotion growth of seedling.Therefore, the straight chain fatty acid that it is 18-22 that the present invention selects carbon chain lengths especially, contain 2-6 unsaturated bond, the polyunsaturated fatty acid that meets this condition all can produce obvious facilitation to growth of seedling after using.
Preferably, described polyunsaturated fatty acid is arachidonic acid, linoleic acid, CLA/alpha-linolenic acid/gamma-Linolenic acid/all-cis formula-4,7,10,13,16,19-DHA/all-cis formula-5,8, the mixing of one or more in 11,14,17-eicosapentaenoic acid.
Above-mentioned several polyunsaturated fatty acids in actual applications, all can make seedling growth rate, adverse-resistant characteristic be greatly improved, and also have advantages of that easy acquisition, cost are easy to control simultaneously, are particularly conducive to and are applied in large-scale agricultural production.Through inventor checking, wherein linoleic acid effect of equal value is especially outstanding, when it can make the biomass of seedling increase high conventional treatment more than one times.
The addition of described polyunsaturated fatty acid is 5-15mL/10 strain seedling.
The addition of polyunsaturated fatty acid is very few, its effectiveness not obvious.Polyunsaturated fatty acid fatty acid addition is too high, easily causes cost to rise.Through inventor checking, when the addition of polyunsaturated fatty acid is every 10 strain seedling 5-15mL, can guarantee that its facilitation to growth of seedling reaches peak, again production control cost effectively.Simultaneously because polyunsaturated fatty acid is not yet clear and definite to the mechanism of action of seedling, put on separately seedling or put on separately in matrix and all can not reach optimum effect, therefore when using polyunsaturated fatty acid, should make polyunsaturated fatty acid put on equably seedling and matrix.Therefore adopt the mode of spraying to add polyunsaturated fatty acid, can be used as preferred version of the present invention.
Further, the inoculum concentration of described Glomus mosseae is a dry weight 0.3-0.5g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
6～5 * 10
8individual pseudomonad.
Due to the facilitation of pseudomonad to plant of Solanaceae growth, only under the effect of Glomus mosseae, can be expressed.If the inoculum concentration of pseudomonad is too high, likely takies the nutriment of seedling and cause seedling morbidity; And pseudomonad inoculum concentration is very few, cannot give play to again the above-mentioned facilitation to growth of seedling.Therefore, can be in implementation process, the real object that promotes growth of seedling, improves its adverse-resistant characteristic that realizes, very important to the selection of Glomus mosseae inoculum concentration and pseudomonad inoculum concentration.In the present invention, every seed inoculation Glomus mosseae 0.3-0.5g, every kind of matrix inoculation 5 * 10 that is implanted with 100 seedling
8～5 * 10
8individual pseudomonad.Under this proportioning, Glomus mosseae can with pseudomonad generation synergistic, farthest promote growth of seedling, improve the adverse-resistant characteristic of seedling, and don't cause the generation of disease because of the flora imbalance of symbiosis.
Before described step a, also comprise the processing that seed is carried out disinfection and soaks seed and make it sprout and show money or valuables one carries unintentionally; The backward matrix of described inoculation pseudomonad is added polyunsaturated fatty acid, refers to 20-30 hour backward matrix interpolation polyunsaturated fatty acid of inoculation pseudomonad.
A large amount of pathogenic microorganismss is contained on the seed top layer of plant of Solanaceae conventionally, if do not reduce its quantity, likely affects the effect of inoculation of follow-up Glomus mosseae, pseudomonad, reduces the facilitation to growth of seedling after the two and seedling symbiosis.Therefore, the present invention adopts the mode of seed soaking to carry out disinfection to seed, and the condition of seed soaking is generally comparatively gentle, can remove the pathogenic microorganisms of the seed top layer overwhelming majority, can also promote the sprouting of seed.Inoculate pseudomonad 20-30 as a child, then add polyunsaturated fatty acid to seedling and matrix
Described seed soaking refers to seed is soaked in to 5-20 minute in the clear water of 20-30 ℃, then seed is soaked in the clear water of 50-70 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1-1.5cm.
Described peat soil is commercially available prod.
the advantage of peat soil is to have concurrently good moisturizing and permeability simultaneously, can promote the growth of root system of plant and the breeding of fungal component.Adopt cave dish to carry out seedling culture, can prevent on the one hand seedling root system in process of growth be mutually wound around and affect in step c by seedling replanting to soil; On the other hand, be also conducive to the fungal component inoculum concentration of seed or seedling to carry out meticulous control, make two kinds of fungal components reach the highest to the facilitation effect of seedling.
preferably, described pseudomonad is pseudomonas fluorescens or pseudomonas aeruginosa.
the present invention, with respect to prior art, mainly has following beneficial effect:
1. the present invention proposes in plant of Solanaceae cultivating process, first backward its seedling inoculation Glomus mosseae and pseudomonad,make cultivated plant of Solanaceae at Seedling Stage, show the adverse-resistant characteristics such as good drought resisting, freeze proof, disease resistance, under the synergy of two kinds of fungal components, described plant of Solanaceae growth of seedling speed also has larger lifting simultaneously; When especially plant of Solanaceae is planted in continuous cropping in field for needs, adopt method of the present invention can effectively avoid the pernicious bacteria infringement seedling gathering in soil, improve the survival rate of seedling.
2. the present invention, simultaneously after inoculation fungal component, sprays polyunsaturated fatty acid to matrix and seedling,through inventor's checking, after inoculation pseudomonad, to matrix and seedling, use after polyunsaturated fatty acid, can stimulate significantly growth of seedling, improve the growth rate of seedling.Meanwhile, the adverse-resistant characteristic such as polyunsaturated fatty acid, disease resistance freeze proof to seedling also has larger facilitation.
Embodiment
For the ease of it will be appreciated by those skilled in the art that below in conjunction with embodiment, the present invention is described in further detail:
Embodiment 1
The present embodiment provides a kind of plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; Described inoculation pseudomonad also comprises the processing of spraying polyunsaturated fatty acid to matrix after 24 hours.
Described Glomus mosseae is commercially available Glomus mosseae microbial inoculum, and described pseudomonas fluorescens is commercially available prod.
The addition of described polyunsaturated fatty acid is 10mL/10 strain seedling.Described polyunsaturated fatty acid is linoleic acid.
Polyunsaturated fatty acid can also be added in clear water, form after uniform emulsion row again and spray.
The inoculum concentration of described Glomus mosseae is a dry weight 0.4g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
6individual pseudomonad.
Described seed soaking refers to seed is soaked in the clear water of 25 ℃ to 10 minutes, then seed is soaked in the clear water of 60 ℃ after 2 hours, then cooling is dipped to it by seed and sprouts and show money or valuables one carries unintentionally naturally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1cm.
Embodiment 2
The present embodiment provides a kind of plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; Described inoculation pseudomonad also comprises the processing of spraying polyunsaturated fatty acid to matrix after 2 hours.
Described Glomus mosseae is commercially available Glomus mosseae microbial inoculum, and described pseudomonas fluorescens is commercially available prod.
The addition of described polyunsaturated fatty acid is 15mL/10 strain seedling.Described polyunsaturated fatty acid is linoleic acid.
Polyunsaturated fatty acid can also be added in clear water, form after uniform emulsion row again and spray.
The inoculum concentration of described Glomus mosseae is a dry weight 0.3g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
7individual pseudomonad.
Described seed soaking refers to seed is soaked in the clear water of 20 ℃ to 20 minutes, then seed is soaked in the clear water of 50 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1.5cm.
Embodiment 3
The present embodiment provides a kind of plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; Described inoculation pseudomonad also comprises the processing of spraying polyunsaturated fatty acid to matrix after 30 hours.
Described Glomus mosseae is commercially available Glomus mosseae microbial inoculum, and described pseudomonas fluorescens is commercially available prod.
The addition of described polyunsaturated fatty acid is 5mL/10 strain seedling.Described polyunsaturated fatty acid is linoleic acid.
Polyunsaturated fatty acid can also be added in clear water, form after uniform emulsion row again and spray.
The inoculum concentration of described Glomus mosseae is a dry weight 0.5g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
8individual pseudomonad.
Described seed soaking refers to seed is soaked in the clear water of 30 ℃ to 5 minutes, then seed is soaked in the clear water of 70 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1.5cm.
Embodiment 4
The present embodiment provides a kind of plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; Described inoculation pseudomonad also comprises the processing of spraying polyunsaturated fatty acid to matrix after 26 hours.
Described Glomus mosseae is commercially available Glomus mosseae microbial inoculum, and described pseudomonas aeruginosa is commercially available prod.
The addition of described polyunsaturated fatty acid is 10mL/10 strain seedling.Described polyunsaturated fatty acid is alpha-linolenic acid.
Polyunsaturated fatty acid can also be added in clear water, form after uniform emulsion row again and spray.
The inoculum concentration of described Glomus mosseae is a dry weight 0.4g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
6individual pseudomonad.
Described seed soaking refers to seed is soaked in the clear water of 29 ℃ to 10 minutes, then seed is soaked in the clear water of 60 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1.3cm.
Embodiment 5
The present embodiment provides a kind of plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; Described inoculation pseudomonad also comprises the processing of spraying polyunsaturated fatty acid to matrix after 20-30 hour.
Described Glomus mosseae is commercially available Glomus mosseae microbial inoculum, and described pseudomonas aeruginosa is commercially available prod.
The addition of described polyunsaturated fatty acid is 8mL/10 strain seedling.Described polyunsaturated fatty acid is alpha-linolenic acid.
Polyunsaturated fatty acid can also be added in clear water, form after uniform emulsion row again and spray.
The inoculum concentration of described Glomus mosseae is a dry weight 0.3g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
7individual pseudomonad.
Described seed soaking refers to seed is soaked in the clear water of 22 ℃ to 15 minutes, then seed is soaked in the clear water of 55 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1-1.5cm.
Embodiment 6
The present embodiment provides a kind of plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; Described inoculation pseudomonad also comprises the processing of spraying polyunsaturated fatty acid to matrix after 26 hours.
Described Glomus mosseae is commercially available Glomus mosseae microbial inoculum, and described pseudomonas aeruginosa is commercially available prod.
The addition of described polyunsaturated fatty acid is 7mL/10 strain seedling.Described polyunsaturated fatty acid is alpha-linolenic acid.
Polyunsaturated fatty acid can also be added in clear water, form after uniform emulsion row again and spray.
The inoculum concentration of described Glomus mosseae is a dry weight 0.5g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
8individual pseudomonad.
Described seed soaking refers to seed is soaked in the clear water of 23 ℃ to 9 minutes, then seed is soaked in the clear water of 65 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1-1.5cm.
Embodiment 7
The present embodiment provides a kind of plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
Before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; Described inoculation pseudomonad also comprises the processing of spraying polyunsaturated fatty acid to matrix after 30 hours.
Described Glomus mosseae is commercially available Glomus mosseae microbial inoculum, and described pseudomonas fluorescens is commercially available prod.
The addition of described polyunsaturated fatty acid is 15mL/10 strain seedling.Described polyunsaturated fatty acid is arachidonic acid.
Polyunsaturated fatty acid can also be added in clear water, form after uniform emulsion row again and spray.
The inoculum concentration of described Glomus mosseae is a dry weight 0.4g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
6individual pseudomonad.
Described seed soaking refers to seed is soaked in the clear water of 25 ℃ to 14 minutes, then seed is soaked in the clear water of 66 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
Described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1-1.5cm.
Reference examples 1
This reference examples provides the breeding method of a kind of plant of Solanaceae, and it does not apply polyunsaturated fatty acid to seedling and matrix.All the other steps are all consistent with embodiment 1.
Reference examples 2
This reference examples provides the breeding method of a kind of plant of Solanaceae, and it is not to seed or the seedling of taking root inoculation Glomus mosseae.All the other steps are all consistent with embodiment 1.
Reference examples 3
This reference examples provides the breeding method of a kind of plant of Solanaceae, and it is not to seed or the seedling of taking root inoculation pseudomonad.All the other steps are all consistent with embodiment 1.
Reference examples 4
This reference examples provides the breeding method of a kind of plant of Solanaceae, and it does not inoculate Glomus mosseae, pseudomonad to seed or the seedling of taking root, and seedling is not used to polyunsaturated fatty acid yet.All the other steps are all consistent with embodiment 1.
Adopt the method for embodiment 1-7, reference examples 1-4 to cultivate commercially available tomato seeds, cultivate 10 for every group, every the average biomass of ten days each groups of test.Result is as shown in table 1.
Adopt the method for embodiment 1-7, reference examples 1-4 to cultivate the tobacco that adopts existing tissue culture method the to obtain seedling of taking root, cultivate 10 for every group, every average biomass of ten days each groups of test.Result is as shown in table 2.
Table 1 tomato is cultivated every 10 days biomasss
| Sample | Cultivate biomass (g/ fresh weight) on the 10th | Cultivate biomass (g/ fresh weight) on the 20th |
| Embodiment 1 | 12.4±1.1 | 21.5±2.1 |
| Embodiment 2 | 14.5±2.5 | 25.3±1.8 |
| Embodiment 3 | 11.9±1.6 | 20.7±2.5 |
| Embodiment 4 | 12.1±1.5 | 21.6±1.7 |
| Embodiment 5 | 12.6±2.3 | 22.4±2.8 |
| Embodiment 6 | 11.8±1.6 | 21.9±2.1 |
| Embodiment 7 | 10.2±1.6 | 20.1±3.2 |
| Reference examples 1 | 8.5±1.4 | 15.3±2.8 |
| Reference examples 2 | 7.4±1.2 | 13.6±2.1 |
| Reference examples 3 | 8.2±1.7 | 14.1±2.4 |
Table 2 tobacco is cultivated every 10 days biomasss
| Sample | Cultivate biomass (g/ fresh weight) on the 10th | Cultivate biomass (g/ fresh weight) on the 20th |
| Embodiment 1 | 7.3±0.6 | 14.2±1.9 |
| Embodiment 2 | 8.9±1.1 | 15.6±2.1 |
| Embodiment 3 | 7.5±0.8 | 14.7±2.5 |
| Embodiment 4 | 8.4±0.4 | 15.8±1.6 |
| Embodiment 5 | 6.9±1.7 | 13.2±2.3 |
| Embodiment 6 | 7.5±0.9 | 15.7±1.7 |
| Embodiment 7 | 7.8±1.3 | 14.6±0.4 |
| Reference examples 1 | 5.2±1.1 | 11.2±0.8 |
| Reference examples 2 | 4.1±0.6 | 10.4±1.6 |
| Reference examples 3 | 4.9±0.5 | 9.9±1.2 |
When plant of Solanaceae introduced disease or when not tolerating adverse circumstances and affecting, its biomass increases less.Therefore can find out from above-mentioned data, adopt method provided by the invention to cultivate plant of Solanaceae, can effectively promote the growth of plant of Solanaceae, improve its adverse-resistant characteristic.
Be more than wherein specific implementation of the present invention, it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these apparent replacement forms all belong to protection scope of the present invention.
Claims (10)
1. a plant of Solanaceae breeding method, comprises the following steps:
A. seed or group are trained to taking root in seedling implantation solid matrix of obtaining, and to matrix inoculation Glomus mosseae;
B. the plumule of seed is sprouted and is exposed to matrix and forms outward after seedling, to matrix inoculation pseudomonad; When the seedling of taking root that group training is obtained is cultivated, when implanting matrix simultaneously to matrix inoculation pseudomonad; After inoculation pseudomonad, obtain the seedling of bacteriumization;
C. seedling culture is extremely ripe.
2. plant of Solanaceae hybridization scheme according to claim 1, is characterized in that: also comprise after inoculation pseudomonad, to matrix and seedling, add polyunsaturated fatty acid.
3. plant of Solanaceae breeding method according to claim 2, is characterized in that: described polyunsaturated fatty acid is the straight chain fatty acid of carbon chain lengths 18-22, and its unsaturated bond quantity is 2-6.
4. plant of Solanaceae breeding method according to claim 3, it is characterized in that: described polyunsaturated fatty acid is arachidonic acid, linoleic acid, CLA/alpha-linolenic acid/gamma-Linolenic acid/all-cis formula-4,7,10,13,16,19-DHA/all-cis formula-5,8,11, the mixing of one or more in 14,17-eicosapentaenoic acid.
5. plant of Solanaceae breeding method according to claim 4, is characterized in that: the addition of described polyunsaturated fatty acid is 5-15mL/10 strain seedling.
6. according to the plant of Solanaceae breeding method described in claim 1-5 any one, it is characterized in that: the inoculum concentration of described Glomus mosseae is a dry weight 0.3-0.5g/ seed or the seedling of taking root; Describedly to matrix inoculation pseudomonad, refer to the matrix inoculation 5 * 10 that is often implanted with 100 seeds or the seedling of taking root
6～5 * 10
8individual pseudomonad.
7. plant of Solanaceae breeding method according to claim 6, is characterized in that: before described step a, also comprise the processing that seed is carried out disinfection, soaks seed and make it sprout and show money or valuables one carries unintentionally; The backward matrix of described inoculation pseudomonad is added polyunsaturated fatty acid, refers to 20-30 hour backward matrix interpolation polyunsaturated fatty acid of inoculation pseudomonad.
8. plant of Solanaceae breeding method according to claim 7, it is characterized in that: described seed soaking refers to seed is soaked in to 5-20 minute in the clear water of 20-30 ℃, again seed is soaked in the clear water of 50-70 ℃ after 2 hours, then seed is dipped to it sprouts and show money or valuables one carries unintentionally.
9. plant of Solanaceae breeding method according to claim 8, is characterized in that: described matrix is peat soil; Describedly seed or the seedling of taking root are implanted to solid matrix refer to seed or the seedling of taking root are implanted in the peat soil being contained in the dish of cave, every dibbling enters 1 seed or the seedling of taking root, and to peat soil spray water, makes it moistening; Described is that sensing peat soil spray concentration is 10 to matrix inoculation pseudomonad
6the pseudomonad suspension of individual/mL; The degree of depth that described seed is implanted peat soil is 1-1.5cm.
10. plant of Solanaceae breeding method, is characterized in that: described pseudomonad is pseudomonas fluorescens or pseudomonas aeruginosa.
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| CN105638151A (en) * | 2014-11-10 | 2016-06-08 | 黑龙江省聚拢乾坤农业技术开发有限公司 | Cultivation method for crops |
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