CN108293504A - A kind of device for raising seedlings and method for cultivating mycorrhizal seedlings - Google Patents
A kind of device for raising seedlings and method for cultivating mycorrhizal seedlings Download PDFInfo
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- CN108293504A CN108293504A CN201810045227.6A CN201810045227A CN108293504A CN 108293504 A CN108293504 A CN 108293504A CN 201810045227 A CN201810045227 A CN 201810045227A CN 108293504 A CN108293504 A CN 108293504A
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 47
- 241000233866 Fungi Species 0.000 claims abstract description 41
- 239000002689 soil Substances 0.000 claims abstract description 31
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 230000000384 rearing effect Effects 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 4
- 239000000645 desinfectant Substances 0.000 claims abstract description 4
- 238000010899 nucleation Methods 0.000 claims abstract description 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims abstract description 3
- 239000011159 matrix material Substances 0.000 claims description 21
- 238000011081 inoculation Methods 0.000 claims description 13
- 235000016709 nutrition Nutrition 0.000 claims description 10
- 230000035764 nutrition Effects 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 239000008223 sterile water Substances 0.000 claims description 7
- 230000012010 growth Effects 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 4
- 230000035784 germination Effects 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 2
- 230000002786 root growth Effects 0.000 claims description 2
- 239000002609 medium Substances 0.000 abstract description 11
- 208000015181 infectious disease Diseases 0.000 abstract description 9
- 238000005406 washing Methods 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 8
- 229920000915 polyvinyl chloride Polymers 0.000 description 8
- 239000004800 polyvinyl chloride Substances 0.000 description 8
- 241000425037 Toona sinensis Species 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 5
- 241000156811 Glomus versiforme Species 0.000 description 4
- 241000235504 Rhizophagus intraradices Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 241000219428 Fagaceae Species 0.000 description 2
- 241000218641 Pinaceae Species 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000209072 Sorghum Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000001863 plant nutrition Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 235000011783 Cedrela sinensis Nutrition 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Abstract
The invention discloses a kind of device for raising seedlings and method for cultivating mycorrhizal seedlings, device is divided into upper layer and lower layer, and upper layer is seedling rearing room, and lower layer is microbial inoculum room；Seedling rearing room upper opening, bottom are mesh-like, are equipped with PVC nets or wire netting that aperture is 2mm；Bush mycorrhizal fungi preparation is placed in microbial inoculum room upper opening, sealed bottom, inside.When nursery, the surface of the seed is sterilized with 10% sodium hypochlorite or other disinfectants, is used in combination aseptic water washing clean；Pressure sterilizing, then seeding and seedling raising are carried out to culture medium or soil.Mycorrhizal seedlings are cultivated using device for raising seedlings of the present invention, seedling medium is not mixed with bush mycorrhizal fungi preparation, microbial inoculum is set only to be contacted with seedling root, increase microbial inoculum density around root system, so that root system is come into full contact with microbial inoculum, improves Mycorrhizal Infection Incidence, reduce other miscellaneous bacterias and infect, seedling medium dosage can be also reduced, cost is reduced.
Description
Technical field
The present invention relates to nursery stock breeding technical fields, specifically, be it is a kind of cultivate mycorrhizal seedlings device for raising seedlings and
Method.
Background technology
Mycorrhizal seedlings refer to that mycorrhizal fungi microbial inoculum is added in seedling medium or rhizosphere soil, makes when cultivating nursery stock
Mycorrhizal fungi infects seedling root, and forms mycorrhizas homobium, is Va Mycorrhiza Seedling by the nursery stock that mycorrhizal fungi is successfully infected.Mycorhiza
Seedling has many advantages, such as that survival rate is high, the speed of growth is fast, resistance is strong for planting trees.Mycorrhizal fungi is divided into Applying Ectomycorrhizal Fungi
And AM fungi, AM fungi are also known as Vesicular arbuscular mycorriza, referred to as arbuscular mycorrhizal fungi.Exotrophic mycorrhiza
Fungi mainly infects a small number of higher plants such as Pinaceae, Fagaceae, and AM fungi can infect most of higher plant.Outside
Raw mycorrhizal fungi microbial inoculum can be widely used by breeding acquisition in vitro in cultivating Pinaceae, Fagaceae nursery stock；And clump branch bacterium
Mycorrhiza fungi can not breed in vitro, can only with could be bred after live plant Root Symbiont, therefore arbuscular mycorrhizal fungi breeding is than outer
The raw mycorrhizal fungi period is long, of high cost.Currently, being by mixing certain ratio in seedling medium using arbuscular mycorrhizal fungi cultivation
The arbuscular mycorrhizal fungus fungal inoculant of example, matrix is placed in seedbed, seed plate or nutrition cup, and then sowing is cultivated.It is this
There is also some defects at present for the Va Mycorrhiza Seedling that method is cultivated：（1）Seedling medium is needed to sterilize before cultivating Va Mycorrhiza Seedling, due to educating
Seedling matrix requirement is big, therefore it is of high cost to sterilize.It is to obtain seedling using group culturation rapid propagating technology that more satisfactory nursery stock, which connects bacterium method,
Wood, aseptically Arbuscular Mycorrhizal Fungi can obtain that infection rate is high, the Va Mycorrhiza Seedling more than quantity.But current big portion
Divide afforestation nursery stock that can not also be obtained by group culturation rapid propagating technology, is mainly obtained using seedbed or nutrition cup nursery, in this case
More using seedling medium, sterilizing is of high cost.（2）Currently, arbuscular mycorrhizal fungi can not be bred in vitro, microbial inoculum expansion numerous period is long,
Such as use seedbed nursery or nutrition cup nursery, need in soil matrix be added larger proportion inoculation microbial inoculum, need microbial inoculum amount compared with
Greatly, cost is higher, and arbuscular mycorrhizal fungi can only be come into full contact with by mycelia and spore with root system of plant can just infect plant roots
System, when using seedbed or nutrition cup nursery, microbial inoculum density is small around root system, and spore and mycelia cannot come into full contact with root system, greatly
It is big to influence effect of inoculation, or even root system can not be infected.
Invention content
The purpose of the present invention is in view of the deficiencies of the prior art, and provide a kind of device for raising seedlings that cultivating mycorrhizal seedlings and
Using the method for culturing seedlings of the device, the method for arbuscular mycorrhizal fungi cultivation mycorrhizal seedlings is specifically utilized.
Realizing the technical solution of the object of the invention is：
A kind of device for raising seedlings for cultivating mycorrhizal seedlings is divided into upper layer and lower layer, and upper layer is seedling rearing room, and lower layer is microbial inoculum room；Nursery
Room upper opening, bottom are mesh-like, are equipped with PVC nets or wire netting that aperture is 2mm；Microbial inoculum room upper opening, sealed bottom,
Place bush mycorrhizal fungi preparation in inside.
The long 40-50cm of seedling rearing room, wide 30-40cm, high 10cm.
The long 40-50cm in microbial inoculum room, wide 30-40cm, high 3cm.
The nursery stock is pricked after seedling rearing room grows a period of time under root system, and wherein radicula of the diameter less than 2mm may extend to
Lower layer's microbial inoculum room, in microbial inoculum room, the spore and mycelial density of arbuscular mycorrhizal fungi are high, and the tender radicula of plant and microbial inoculum are abundant
Contact greatly improves Mycorrhizal Infection Incidence, shortens the mycorrhiza fungi seeding cultivating time.
Device for raising seedlings of the present invention can be such that seedling medium is detached with inoculation microbial inoculum, and high density inoculation is formed at plant fine root position
Agent.
The method for cultivating mycorrhizal seedlings using device for raising seedlings of the present invention, includes the following steps：
（1）Prepare inoculation bush mycorrhizal fungi preparation：Can buy or voluntarily screen expand it is numerous, need ensure microbial inoculum have live
Property；
（2）Prepare the seed for needing to cultivate nursery stock：It can buy or oneself is acquired, need to ensure that seed has vigor, Neng Goufa
Bud；Before sowing, the surface of the seed is sterilized with 10% sodium hypochlorite or other disinfectants, is used in combination aseptic water washing clean；
（3）Prepare culture medium or soil：It is needed voluntarily to prepare matrix or soil according to seedling growth, it is big to remove diameter in matrix
In impurity such as the chads, root system, leaf of 2cm；Matrix is packed into cloth bag, pressure sterilizing is carried out at 121 DEG C 2 times, each 30min；
（4）The matrix after sterilizing or soil are added in seedling rearing room：The matrix or thickness of soil of addition are 3-4cm, then in base
Seed in matter or soil after one layer of uniform broadcasting disinfection, covers the sterilization matrix or soil of 3-4cm thickness on seed again, sprinkle without
Bacterium water was applied every 1-2 weeks and spills one time of nutrition liquid, and conventional cultivation is carried out in greenhouse；
（5）According to nursery stock germination and growth time, root growth situation is observed, after root system extends seedling rearing room bottom, in bacterium
Ready bush mycorrhizal fungi preparation is added in agent room, and a layer thickness is tiled as 2-3cm microbial inoculums in microbial inoculum room；During cultivating nursery stock
It is sprayed with sterile water, avoids using tap water, in order to avoid there is miscellaneous bacteria to influence effect of inoculation；3-4 are cultivated in greenhouse or shade
After month, mycorrhiza fungi seeding cultivating is completed, then takes out nursery stock, transplanting carries out transplanting afforestation to nutrition cup or directly.
Present invention mainly solves two problems：
（1）Seedling medium is not mixed with bush mycorrhizal fungi preparation, and microbial inoculum is made only to be contacted with seedling root, increases bacterium around root system
Agent density, makes root system be come into full contact with microbial inoculum, improves root invasion rate；
（2）Seedling medium dosage is reduced, matrix sterilizing cost is reduced.
Mycorrhizal seedlings are cultivated using device for raising seedlings of the present invention, Mycorrhizal Infection Incidence can be improved, reduce other miscellaneous bacterias and infect, subtract
Few substrates quantity, reduces cost.
Description of the drawings
Fig. 1 is the structural schematic diagram of device for raising seedlings of the present invention.
In figure, 1. seedling rearing room 1-1.PVC nets, 2. microbial inoculum room 2-1. bush mycorrhizal fungi preparations.
Specific implementation mode
The content of present invention is further described with reference to embodiment and attached drawing, but is not with the restriction of the present invention.
Embodiment 1
A kind of device for raising seedlings for cultivating mycorrhizal seedlings is divided into upper layer and lower layer, and upper layer is seedling rearing room, and lower layer is microbial inoculum room；Nursery
Room upper opening, bottom are mesh-like, are equipped with the PVC nets that aperture is 2mm；Microbial inoculum room upper opening, sealed bottom, inside are placed
Bush mycorrhizal fungi preparation.
Specifically production method is：Prepare thickness be 0.5cm PVC board, be respectively cut out size be 30cm × 40cm,
The PCV plates of 30cm × 10cm, 40cm × 10cm, 30cm × 3cm, 40cm × 3cm.With polyvinyl chloride adhesive, by what is be cut into
PVC board is bonded into upper opening, and the length of bottom lock is respectively the cuboid box of 40cm, 30cm, 10cm as educating
Seedling room punches in the PVC board of its bottom surface 30cm × 40cm, aperture 2mm, pitch of holes 0.5cm, and it is mesh-like to make bottom surface.With poly-
PVC board is bonded into upper opening by vinyl chloride adhesive, and the length of bottom lock is respectively the length of 40cm, 30cm, 3cm
Cube box, it is internal to place bush mycorrhizal fungi preparation as microbial inoculum room.
The method for cultivating Mycorrhizal top fruit tree nursery stock using above-mentioned device for raising seedlings, includes the following steps：
（1）Prepare inoculation bush mycorrhizal fungi preparation:Glomus intraradices（Glomus intraradices, BGC JX04B）With
Glomus versiforme（Glomus versiforme, BGC XJ08F）From Inst. of Plant Nutrition ＆. Resource, Beijing City Academy of Agricultural ＆. Fo
Purchase；In greenhouse, microbial inoculum is carried out to expand numerous culture using the boiling sand culture substrate and Sorghum Seedlings of sterilizing, microbial inoculum is bred 3 months
Rhizosphere soil and root system are harvested afterwards, air-dry and mycorrhizal fungi Inoculant, microbial inoculum is used as to contain spore, mycelia and mycorhiza root after crushing
Section, it is spare as inoculation microbial inoculum after the bush mycorrhiza agent for expanding numerous culture is mixed（Mix bacterium agent spore content is 28/g）；
（2）Prepare top fruit tree seed：From Kunming, beauteously nursery stock breeding Co., Ltd buys, and the sodium hypochlorite with a concentration of 10 % is molten
10 min of liquid disinfectant, then with spare after aseptic water washing 3 times；
（3）Prepare nursery soil：Using nursery soil as seedling medium, crosses 2cm and weed out except bulk chad, root system and leaf etc.
Sundries；Matrix is distributed into cloth bag, pressure sterilizing is carried out at 121 DEG C 2 times, each 30min；Soil matrix after sterilizing is spare.
（4）Soil after sterilizing is added in seedling rearing room：The thickness of soil of addition is 3-4cm, is then uniformly spread on soil
The top fruit tree seed after one layer of disinfection is broadcast, covers the soil after the sterilizing of 3-4cm thickness on seed again, sprinkle sterile water, and every
It applies within 1-2 weeks and spills one time of nutrition liquid, conventional cultivation is carried out in greenhouse；
（5）Fruit tree seed is pushed up in one week inland supervention bud, seedling radicula grows seedling rearing room bottom in 15 days；It is added in microbial inoculum room
1.5kg above-mentioned steps（1）The bush mycorrhizal fungi preparation prepared（28/g of spore content）, microbial inoculum thickness 2-3cm, addition bacterium
After agent, nursery stock continues culture 3 months in greenhouse, is irrigated with sterile water during culture, in order to avoid there is miscellaneous bacteria to influence effect of inoculation；
After culture 3 months, 10 seedlings root systems is taken to carry out Mycorrhizal Infection Incidence detection, seedlings root Mycorrhizal Infection Incidence reaches 46.5%, at this time
Va Mycorrhiza Seedling can carry out transplanting afforestation.
Embodiment 2
The method for cultivating Mycorrhizal Seedlings of Different Toona sinensis Provenances using 1 device for raising seedlings of embodiment, includes the following steps：
（1）Prepare inoculation bush mycorrhizal fungi preparation：It is bought from Inst. of Plant Nutrition ＆. Resource, Beijing City Academy of Agricultural ＆. Fo
Glomus intraradices（Glomus intraradices, BGC JX04B）And Glomus versiforme（Glomus versiforme, BGC
XJ08F）；In greenhouse, microbial inoculum is carried out to expand numerous culture using the boiling sand culture substrate and Sorghum Seedlings of sterilizing, microbial inoculum breeds 3
Harvest rhizosphere soil and root system after month air-dry and are inoculated with microbial inoculum as mycorhiza after crushing, and microbial inoculum contains spore, mycelia and mycorhiza root
Section, it is spare as inoculation microbial inoculum after the bush mycorrhiza agent for expanding numerous culture is mixed（Mix bacterium agent spore content is 28/g）；
（2）Prepare Chinese toon seed：From Kunming, beauteously nursery stock breeding Co., Ltd buys, with the liquor natrii hypochloritis of a concentration of 10 %
Sterilize 10 min, then with spare after aseptic water washing 3 times；
（3）Prepare nursery soil base：Using nursery soil as seedling medium, crosses 2cm and weed out except bulk chad, root system and leaf
Equal sundries；Matrix is distributed into cloth bag, pressure sterilizing is carried out at 121 DEG C 2 times, each 30min；Soil matrix after sterilizing is standby
With；
（4）Soil after sterilizing is added in seedling rearing room：The thickness of soil of addition is 3-4cm, then uniform broadcasting one on soil
Chinese toon seed after layer disinfection, the soil after the sterilizing of 3-4cm thickness, sprinkle sterile water is covered on seed, and applied every 1-2 weeks again
One time of nutrition liquid is spilt, conventional cultivation is carried out in greenhouse；
（5）Chinese toon seed germinates successively within 2 weeks, and 30 days or so seedling radiculas extend seedling rearing room bottom after germination；At this point,
1.5kg above-mentioned steps are added in microbial inoculum room（1）The bush mycorrhizal fungi preparation prepared（28/g of spore content）, microbial inoculum thickness
2-3cm continues culture 3 months after microbial inoculum is added in greenhouse, is irrigated with sterile water during culture, in order to avoid there is miscellaneous bacteria to influence inoculation
Effect；After culture 3 months, 10 seedlings root systems is taken to carry out infection rate detection, seedlings root Mycorrhizal Infection Incidence reaches 52.7%,
Va Mycorrhiza Seedling at this time can carry out transplanting afforestation.
Using embodiment 1, implement the method for 2 device for raising seedlings cultivation mycorrhizal seedlings and existing cultivation mycorrhizal seedlings side
Method compares, to cultivate 1000 plants of stony desertification afforestation with mycorhiza nursery stock（Push up fruit tree or Chinese toon）For row：
By the above comparative illustration, mycorrhizal seedlings are cultivated using device for raising seedlings of the present invention, Mycorrhizal Infection Incidence can be improved, reduce it
Its miscellaneous bacteria is infected, and is reduced matrix and microbial inoculum dosage, is reduced cost.
Claims (3)
1. a kind of device for raising seedlings for cultivating mycorrhizal seedlings, it is characterised in that：The device is divided into upper layer and lower layer, and upper layer is nursery
Room, lower layer are microbial inoculum room；Seedling rearing room upper opening, bottom are mesh-like, are equipped with PVC nets or wire netting that aperture is 2mm；Microbial inoculum
Bush mycorrhizal fungi preparation is placed in room upper opening, sealed bottom, inside.
2. device for raising seedlings according to claim 1, it is characterised in that：The long 40-50cm of seedling rearing room, the wide 30-40cm are high
10cm；The long 40-50cm in microbial inoculum room, wide 30-40cm, high 3cm.
3. the method that device for raising seedlings according to claim 1 cultivates mycorrhizal seedlings, includes the following steps：
Prepare inoculation bush mycorrhizal fungi preparation；
（2）Prepare the seed for needing to cultivate nursery stock：The surface of the seed is sterilized with 10% sodium hypochlorite or other disinfectants, nothing is used in combination
Bacterium water is rinsed well；
（3）Prepare culture medium or soil：It is needed voluntarily to prepare matrix or soil according to seedling growth, it is big to remove diameter in matrix
In the chad of 2cm, root system, leaf impurity；Matrix is packed into cloth bag, pressure sterilizing is carried out at 121 DEG C 2 times, each 30min；
（4）The matrix after sterilizing or soil are added in seedling rearing room：The matrix or thickness of soil of addition are 3-4cm, then in base
Seed in matter or soil after one layer of uniform broadcasting disinfection covers matrix or soil after the sterilizing of 3-4cm thickness on seed, drenches again
Sterile water is spilt, was applied every 1-2 weeks and spills one time of nutrition liquid, conventional cultivation is carried out in greenhouse；
（5）According to nursery stock germination and growth time, root growth situation is observed, after root system extends seedling rearing room bottom, in bacterium
Ready bush mycorrhizal fungi preparation is added in agent room, and a layer thickness is tiled as 2-3cm microbial inoculums in microbial inoculum room；It is sprayed during culture
Sterile water completes mycorrhiza fungi seeding cultivating after cultivating 3-4 months in greenhouse or shade, then takes out nursery stock, transplanting to nutrition cup
Or directly carry out transplanting afforestation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810045227.6A CN108293504B (en) | 2018-01-17 | 2018-01-17 | Method for cultivating mycorrhizal nursery stock |
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|---|---|---|---|
| CN201810045227.6A CN108293504B (en) | 2018-01-17 | 2018-01-17 | Method for cultivating mycorrhizal nursery stock |
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| CN108293504A true CN108293504A (en) | 2018-07-20 |
| CN108293504B CN108293504B (en) | 2020-02-11 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197379A (en) * | 2018-11-12 | 2019-01-15 | 贵州大学 | A kind of efficient monospore expanding propagation method of arbuscular mycorrhizal fungi |
| CN109479687A (en) * | 2018-10-23 | 2019-03-19 | 云南省林业科学院 | A kind of China fir mycorrhiza fungi seeding cultivating method |
| CN109729901A (en) * | 2019-03-06 | 2019-05-10 | 云南省林业科学院 | A kind of Anlnus nepalensis mycorrhiza fungi seeding cultivating method |
| CN110301290A (en) * | 2019-07-26 | 2019-10-08 | 金埔园林股份有限公司 | A kind of device and method of rapid synthesis exotrophic mycorrhiza |
| CN112470750A (en) * | 2020-11-18 | 2021-03-12 | 山东农业大学 | Device for conveniently and rapidly detecting mycorrhiza infection rate of plants in batches and using method thereof |
| CN115500178A (en) * | 2022-10-25 | 2022-12-23 | 贵州大学 | Bird Wang Chajun seedling raising device and using method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843073A (en) * | 2006-05-15 | 2006-10-11 | 中国农业科学院蔬菜花卉研究所 | Facility vegetable mycorrhiza production method |
| CN105420125A (en) * | 2015-12-30 | 2016-03-23 | 保定学院 | Arbuscular mycorrhizal fungal inoculant |
| CN105493945A (en) * | 2015-12-15 | 2016-04-20 | 重庆亘宇园林景观工程有限公司 | Seedling culture device |
| CN105861315A (en) * | 2015-11-22 | 2016-08-17 | 南京金埔园林股份有限公司 | Method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi |
| CN107567873A (en) * | 2017-10-13 | 2018-01-12 | 安徽乐林农林科技有限公司 | A kind of environmentally friendly automatic water replenishing type container for plant growth and the method with the container for plant growth serike |
-
2018
- 2018-01-17 CN CN201810045227.6A patent/CN108293504B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843073A (en) * | 2006-05-15 | 2006-10-11 | 中国农业科学院蔬菜花卉研究所 | Facility vegetable mycorrhiza production method |
| CN105861315A (en) * | 2015-11-22 | 2016-08-17 | 南京金埔园林股份有限公司 | Method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi |
| CN105493945A (en) * | 2015-12-15 | 2016-04-20 | 重庆亘宇园林景观工程有限公司 | Seedling culture device |
| CN105420125A (en) * | 2015-12-30 | 2016-03-23 | 保定学院 | Arbuscular mycorrhizal fungal inoculant |
| CN107567873A (en) * | 2017-10-13 | 2018-01-12 | 安徽乐林农林科技有限公司 | A kind of environmentally friendly automatic water replenishing type container for plant growth and the method with the container for plant growth serike |
Non-Patent Citations (3)
| Title |
|---|
| 张中峰等: "接种丛枝菌根真菌对青冈栎幼苗生长和光合作用的影响", 《广西植物》 * |
| 张中峰等: "接种菌根真菌对青冈栎幼苗耐旱性的影响", 《生态学报》 * |
| 张中峰等: "水分胁迫和接种菌根真菌对青冈栎根系形态的影响", 《生态学杂志》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109479687A (en) * | 2018-10-23 | 2019-03-19 | 云南省林业科学院 | A kind of China fir mycorrhiza fungi seeding cultivating method |
| CN109197379A (en) * | 2018-11-12 | 2019-01-15 | 贵州大学 | A kind of efficient monospore expanding propagation method of arbuscular mycorrhizal fungi |
| CN109197379B (en) * | 2018-11-12 | 2020-11-13 | 贵州大学 | Efficient single-spore propagation method for arbuscular mycorrhizal fungi |
| CN109729901A (en) * | 2019-03-06 | 2019-05-10 | 云南省林业科学院 | A kind of Anlnus nepalensis mycorrhiza fungi seeding cultivating method |
| CN110301290A (en) * | 2019-07-26 | 2019-10-08 | 金埔园林股份有限公司 | A kind of device and method of rapid synthesis exotrophic mycorrhiza |
| CN112470750A (en) * | 2020-11-18 | 2021-03-12 | 山东农业大学 | Device for conveniently and rapidly detecting mycorrhiza infection rate of plants in batches and using method thereof |
| CN112470750B (en) * | 2020-11-18 | 2022-05-06 | 山东农业大学 | Device for conveniently and rapidly detecting mycorrhiza infection rate of plants in batches and using method thereof |
| CN115500178A (en) * | 2022-10-25 | 2022-12-23 | 贵州大学 | Bird Wang Chajun seedling raising device and using method thereof |
| CN115500178B (en) * | 2022-10-25 | 2024-01-09 | 贵州大学 | Bird king tea mycorrhiza seedling raising device and application method thereof |
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