CN112470750A - Device for conveniently and rapidly detecting mycorrhiza infection rate of plants in batches and using method thereof - Google Patents
Device for conveniently and rapidly detecting mycorrhiza infection rate of plants in batches and using method thereof Download PDFInfo
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- CN112470750A CN112470750A CN202011289798.8A CN202011289798A CN112470750A CN 112470750 A CN112470750 A CN 112470750A CN 202011289798 A CN202011289798 A CN 202011289798A CN 112470750 A CN112470750 A CN 112470750A
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Abstract
The application discloses a device for conveniently detecting the infection rate of plant mycorrhiza in batches and a using method thereof, wherein a container suspension bracket is arranged at the end part of an experimental solvent container, the first end of the container suspension bracket is movably arranged at the end part of the experimental solvent container, and the second end of the container suspension bracket extends into the experimental solvent container; the second end of the container suspension bracket is connected with a storage plate, and a gap is formed between the edge of the storage plate and the inner wall of the experimental solvent container; be provided with uncovered cylindrical container on putting the thing board, and its part that is located to put between thing board and the experiment solvent container bottom is provided with the hole of permeating water, has the clearance between uncovered cylindrical container bottom and the experiment solvent container bottom. The hole of permeating water of uncovered cylindrical container can effectively guarantee that the reagent infiltration flows, and does not lose plant roots, improves sample treatment efficiency. There is the clearance between uncovered cylindrical container bottom hole end of permeating water and the experiment solvent container bottom on guaranteeing ventilative basis, utilizes the bottom hole of permeating water, keeps away from the heat source relatively simultaneously, has protected plant roots's integrality.
Description
Technical Field
The application relates to the technical field of agricultural experiments, in particular to a device for conveniently and rapidly detecting the mycorrhiza infection rate of plants in batches and a using method thereof.
Background
Mycorrhiza is a symbiont of some fungi and plant roots in soil, and has the main functions of expanding the absorption surface of the root system, increasing the absorption of the root system to soil elements, improving the growth and development of crops, enhancing the stress resistance and disease resistance of plants, particularly the disease resistance of soil-borne diseases, and maintaining the stability of the micro-ecological environment of the plant rhizosphere.
Mycorrhizal growth condition observation and infection rate determination are important basic work in mycorrhizal research. Although the dyeing method is continuously updated, the treatment of the plant root system in the infection rate determination process still needs the pouring and cleaning work of the reagent for many times, which wastes time and labor. Reagent is emptyd and is leaded to plant roots to flow along with the reagent easily, causes the root system to run off. Meanwhile, in the water bath heating process, the temperature of the test container is increased, the steam environment causes difficulty in taking, the evaporation condition of the solvent is serious, and the accuracy of the test result is influenced.
Disclosure of Invention
In order to solve the technical problems, the following technical scheme is provided:
in a first aspect, an embodiment of the present application provides a device for conveniently detecting a mycorrhiza infection rate of a plant in batches, including: the container hanging bracket is arranged at the end part of the experimental solvent container, the first end of the container hanging bracket is movably arranged at the end part of the experimental solvent container, and the second end of the container hanging bracket extends into the experimental solvent container; the second end of the container hanging bracket is connected with an object placing plate, and a gap is formed between the edge of the object placing plate and the inner wall of the experimental solvent container; the experiment solvent container is characterized in that a uncovered cylindrical container is arranged on the object placing plate, a water permeable hole is formed in the part, located between the object placing plate and the bottom of the experiment solvent container, of the uncovered cylindrical container, and a gap exists between the bottom of the uncovered cylindrical container and the bottom of the experiment solvent container.
By adopting the implementation mode, when the infection rate of the plant mycorrhiza is detected, the uncovered cylindrical container can be placed into the beaker filled with the reagent by adopting the container suspension bracket and the storage plate, and the whole device can be directly lifted out when the uncovered cylindrical container is taken out. Because the hole of permeating water of uncovered cylindrical container can effectively guarantee that the reagent infiltration flows out, and does not lose plant roots, improves sample treatment efficiency. And there is the clearance between uncovered cylindrical container bottom hole end of permeating water and the experiment solvent container bottom on guaranteeing ventilative basis, make full use of bottom hole of permeating water, keep away from the heat source relatively simultaneously, further protected the integrality of plant roots.
With reference to the first aspect, in a first possible implementation manner of the first aspect, the article placing plate is provided with 6N article placing holes, N is a positive integer greater than 1, and the uncovered cylindrical container is disposed in the article placing hole. The number of the object placing holes is set to be multiple of 6, so that symmetry can be guaranteed, and the three-time repetition principle of an experiment can be completed. And once put into experiment solvent container with a plurality of uncovered cylindrical container in, realize the batch processing of root system, further improved the treatment effeciency of root system sample.
With reference to the first possible implementation manner of the first aspect, in a second possible implementation manner of the first aspect, a protruding ring is disposed on the uncovered cylindrical container, and a diameter of the protruding ring is larger than a diameter of the storage hole. Because the diameter of the protruding ring is larger than that of the object placing hole, the arrangement of the protruding ring ensures that the uncovered cylindrical container can be clamped in the object placing hole. The protruding ring divides the uncovered cylindrical container into an upper part and a lower part, one part is arranged above the object placing plate, the other part is arranged below the object placing plate, and the part arranged below the object placing plate is provided with water permeable holes for permeating reagent solution. Therefore, when the plant root system is treated, the plant root system is put into the uncovered cylindrical container, but the height of the plant root system does not exceed the height of the storage plate.
With reference to the second possible implementation manner of the first aspect, in a third possible implementation manner of the first aspect, a cover plate is further disposed on the container suspension bracket, air outlets are disposed on the cover plate, the number of the air outlets is the same as that of the storage holes, and the air outlets are correspondingly disposed, and a gap exists between the edge of the cover plate and the inner wall of the experimental solvent container. The cover plate is mainly arranged for pressing the uncovered cylindrical container to prevent the uncovered cylindrical container from toppling over. Set up the venthole on the apron to with the venthole with put the thing hole and correspond the setting, be for plant roots in heating process, the steam that gives out from the cylindrical container of uncovered can discharge, experiment solvent sets up for the drop of water for the one side that thing board and apron correspond experiment solvent container bottom is condensed to the apron because of the gas of being heated the evaporation in the experiment solvent container through putting the thing board and apron moreover, flows back to in the experiment solvent container after the cooling, and then has reduced the evaporation of experiment solvent.
With reference to the third possible implementation manner of the first aspect, in a fourth possible implementation manner of the first aspect, the container suspension bracket includes a cross support frame and a suspension column, one end of the suspension column is movably connected to the cross support frame, the other end of the suspension column is movably connected to the object placing plate, and the cover plate is movably disposed on the suspension column between the cross support frame and the object placing plate. The cross support frame is arranged at the end part of the experimental solvent container to play a supporting role, and the suspension columns are arranged on the cross support frame to realize the suspension of the opposite object rack.
With reference to the fourth possible implementation manner of the first aspect, in a fifth possible implementation manner of the first aspect, the cross-shaped support frame is provided with a first screw hole, the object placing plate is provided with a second screw hole, two ends of the suspension column are respectively provided with external threads corresponding to the first screw hole and the second screw hole, and the suspension column is respectively in threaded connection with the cross-shaped support frame and the object placing plate. Hang the post respectively with cross support frame with put thing board threaded connection for hang the post and can dismantle in a flexible way with cross support frame and put the thing board, improved experimental apparatus's equipment and dismantlement efficiency, and then improved test efficiency.
With reference to the fifth possible implementation manner of the first aspect, in a sixth possible implementation manner of the first aspect, the cross section of the suspension column main body is non-circular or non-quasi-circular, a non-circular hole or a non-quasi-circular hole corresponding to the cross section of the suspension column main body is disposed in the center of the cover plate, and the cover plate is movably connected to the suspension column. The size of the non-circular or non-circular hole is larger than the cross section of the suspension post body, so that the suspension post can penetrate through the cover plate, and the cover plate can move up and down on the suspension post. But the gap between the edge of the non-circular or non-circular-like hole and the suspension post body surface is relatively small. The non-circular or non-circular-like apertures in this embodiment may be square and pentagonal, such that the cover plate cannot rotate around the suspension posts.
With reference to the first aspect or any one of the first to sixth possible implementation manners of the first aspect, in a seventh possible implementation manner of the first aspect, a frosted writing area is arranged on the uncovered cylindrical container. The frosted writing area can be used for marking and recording samples during experiments, and the frosted writing area can be used for avoiding erosion of steam.
With reference to the seventh possible implementation manner of the first aspect, in an eighth possible implementation manner of the first aspect, a gap between the bottom of the uncovered cylindrical container and the bottom of the experimental solvent container is 3-5mm, and a gap between the edge of the object placing plate and the cover plate and the inner wall of the experimental solvent container is 1-3 mm.
In a second aspect, an embodiment of the present application provides a method for using a device for conveniently detecting a mycorrhiza infection rate of a plant in batch, where the device according to the first aspect or any one of the possible implementation manners of the first aspect is adopted, and the method includes: the container hanging bracket, the object placing plate and the cover plate are assembled according to the object placing holes and the air holes. And putting the plant root system into the uncovered cylindrical container, then lifting the cover plate, putting the uncovered cylindrical container with the plant root system into the storage hole in the storage plate, and putting down the cover plate. And (3) putting the cylindrical container with the cover into an experimental solvent container containing an experimental solvent through a container hanging bracket to carry out heating operation. And taking out the cylindrical container with the cover through the container hanging bracket after heating.
Drawings
Fig. 1 is a schematic structural diagram of an apparatus for conveniently detecting a mycorrhiza infection rate of a plant in batch according to an embodiment of the present application;
fig. 2 is a schematic structural view of a storage plate according to an embodiment of the present disclosure;
FIG. 3 is a schematic structural diagram of a capless cylindrical container according to an embodiment of the present disclosure;
fig. 4 is a schematic structural diagram of a cover plate according to an embodiment of the present disclosure;
FIG. 5 is a schematic view of a combination of a suspension post, a cover plate, and a storage plate according to an embodiment of the present disclosure;
fig. 6 is a schematic flow chart of a method for using the apparatus for conveniently detecting the mycorrhiza infection rate of plants in batches according to the embodiment of the present application;
in fig. 1 to 6, the symbols are represented as:
1-experimental solvent container, 2-object placing plate, 3-uncovered cylindrical container, 4-water permeable hole, 5-object placing hole, 6-protruding ring, 7-frosted writing area, 8-cover plate, 9-air outlet hole, 10-cross support frame, 11-suspension column, 12-first screw hole, 13-second screw hole, 14-external screw thread and 15-square hole.
Detailed Description
The present invention will be described with reference to the accompanying drawings and embodiments.
Fig. 1 is a schematic structural diagram of a device for conveniently and rapidly detecting a plant mycorrhiza infection rate in batches according to an embodiment of the present application, and referring to fig. 1, the device for conveniently and rapidly detecting a plant mycorrhiza infection rate in batches according to the embodiment of the present application includes: experiment solvent container 1, 1 tip of experiment solvent container is provided with container suspension support, container suspension support's first end activity sets up 1 tip of experiment solvent container, the second end stretches into inside 1 of experiment solvent container. The second end of container suspension support connects one and puts thing board 2, there is the clearance at 2 edges of putting thing board and 1 inner walls of experiment solvent container. The experiment solvent container is characterized in that an uncovered cylindrical container 3 is arranged on the object placing plate 2, a water permeable hole 4 is formed in the part, between the object placing plate 2 and the bottom of the experiment solvent container 1, of the uncovered cylindrical container 3, and a gap exists between the bottom of the uncovered cylindrical container 3 and the bottom of the experiment solvent container 1. Specifically, the gap between the bottom of the uncovered cylindrical container 3 and the bottom of the experimental solvent container 1 is 3-5mm, and the gap between the edge of the object placing plate 2 and the inner wall of the experimental solvent container 1 is 1-3 mm.
The object placing plate 2 is provided with 6N object placing holes 5, N is a positive integer larger than 1, and the uncovered cylindrical container 3 is arranged in the object placing holes 5. The number of the object placing holes 5 is set to be multiple of 6, so that symmetry can be guaranteed, and the three-time repetition principle of an experiment can be completed. And once put into experiment solvent container 1 with a plurality of uncovered cylindrical container 3 in, realize the batch processing of root system, further improved the treatment effeciency of root system sample. Referring to fig. 2, 18 storage holes 5 are provided.
Referring to fig. 3, a protruding ring 6 is arranged on the uncovered cylindrical container 3, and the diameter of the protruding ring 6 is larger than that of the object placing hole 5. The diameter of the protruding ring 6 is larger than that of the object placing hole 5, so that the arrangement of the protruding ring 6 ensures that the uncovered cylindrical container 3 can be clamped in the object placing hole 5. The protruding ring 6 divides the uncovered cylindrical container 3 into an upper part and a lower part, one part is arranged on the object placing plate 2, the other part is arranged under the object placing plate 2, and the part arranged under the object placing plate 2 is provided with a water permeable hole 4 for permeating reagent solution. Therefore, when the plant root system is treated, the plant root system is put into the uncovered cylindrical container 3, but the height of the storage plate 2 is not exceeded. And the uncovered cylindrical container 3 is provided with a frosted writing area 7. The frosted writing area 7 can record the mark of the sample when the experiment is carried out, and the frosted writing area 7 can avoid the erosion of steam.
The container suspension bracket is further provided with a cover plate 8, referring to fig. 4, the cover plate 8 is provided with air outlets 9, the number of the air outlets 9 is equal to that of the object placing holes 5, the air outlets are correspondingly arranged, a gap exists between the edge of the cover plate 8 and the inner wall of the experimental solvent container 1, and the gap distance is 1-3 mm. The cover plate 8 is provided mainly for the purpose of pressing the capless cylindrical container 3 against the same, preventing the capless cylindrical container 3 from toppling over. Set up venthole 9 on apron 8, and correspond setting up venthole 9 and put thing hole 5, be in order to plant roots in the heating process, the steam that gives out from uncovered cylindrical container 3 can discharge, and experiment solvent is because of the gas of being heated the evaporation in 1 in the experiment solvent container is put thing board 2 and apron 8 and can be condensed into the drop of water in the one side that thing board 2 and apron 8 correspond experiment solvent container 1 bottom putting the thing, the reflux is in experiment solvent container 1 after the cooling, and then the evaporation of experimental solvent has been reduced.
Further referring to fig. 1, the container hanging bracket includes a cross support frame 10 and a hanging column 11, one end of the hanging column 11 is movably connected with the cross support frame 10, the other end of the hanging column 11 is movably connected with the object placing plate 2, and the cover plate 8 is movably arranged on the hanging column 11 between the cross support frame 10 and the object placing plate 2. The cross support frame 10 is arranged at the end part of the experimental solvent container 1 to play a supporting role, and the suspension columns 11 are arranged on the cross support frame 10 to realize the suspension of the opposite object rack.
The cross support frame 10 is provided with a first screw hole 12, the object placing plate 2 is provided with a second screw hole 13, referring to fig. 5, two ends of the suspension column 11 are respectively provided with an external thread 14 corresponding to the first screw hole 12 and the second screw hole 13, and the suspension column 11 is respectively in threaded connection with the cross support frame 10 and the object placing plate 2. Hang post 11 respectively with cross support frame 10 with put 2 threaded connection of thing board for hang post 11 and cross support frame 10 and put thing board 2 and can dismantle in a flexible way, improved experimental apparatus's equipment and dismantlement efficiency, and then improved test efficiency.
The cross section of the suspension column main body is non-circular or non-quasi-circular, the center of the cover plate is provided with a non-circular hole or a non-quasi-circular hole corresponding to the cross section of the suspension column main body, and the cover plate is movably connected with the suspension column. The size of the non-circular or non-circular hole is larger than the cross section of the suspension post body, so that the suspension post can penetrate through the cover plate, and the cover plate can move up and down on the suspension post. But the gap between the edge of the non-circular or non-circular-like hole and the suspension post body surface is relatively small. The non-circular or non-circular-like apertures in this embodiment may be square and pentagonal, such that the cover plate cannot rotate around the suspension posts.
Referring to fig. 4 and 5, in this embodiment, the cross section of the main body of the suspension column 11 is square, a square hole 15 is disposed in the center of the cover plate 8, and the cover plate 8 is movably connected to the suspension column 11 through the square hole 15. The square hole 15 is larger than the cross section of the main body of the suspension post 11, so that the suspension post 11 can pass through the cover plate 8, and the cover plate 8 can move up and down on the suspension post 11. But the clearance between the edge of the square hole 15 and the surface of the suspension post 11 is relatively small and the hole above it is the square hole 15, thus making the cover plate 8 unable to rotate around the suspension post 11.
The experimental solvent container 1 adopted in the specific experimental process is a beaker, and the commonly used beakers are 1000ml and 2000ml in specification, and the parameters of each part of the device for conveniently and massively detecting the plant mycorrhiza infection rate in the specific experiment provided by the embodiment of the application are explained below aiming at the beakers with the two models.
A2000 mL beaker was used as the experimental solvent container 1, and the uncovered cylindrical container 3 was 50mm high, 19mm in outer diameter of the lower bottom surface, 1mm in thickness, and divided into upper and lower portions by the protruding ring 6. The uncovered cylindrical container 3 is designed with a square frosted writing area 7 with the length of 10mm, the width of 10mm, the distance from the upper edge of the container to the upper edge of 5mm and the distance from the lower edge of the protruding ring 6 to the upper edge of 4 mm. The width of the protruding ring 6 is 1mm, the protruding height is 3mm, the upper edge is 19mm away from the upper edge of the uncovered cylindrical container 3, and the lower edge is 30mm away from the lower bottom surface of the uncovered cylindrical container 3. The lower half part and the lower bottom surface of the uncovered cylindrical container 3 are uniformly distributed with the pore diameter of 0.1mm and the density of 150/cm2And water permeable holes 4. The container suspension bracket is composed of a suspension column 11 and a cross-shaped support frame 10, and the object placing plate 2 and the cover plate 8 are arranged on the suspension column 11. Wherein put the thing board 2 and hang the one end of post 11 and be connected, put thing board 2 thickness and be 2mm, the diameter is 125mm, goes up to have the diameter to put the thing hole 524 that 20mm is, puts the thing board 2 intermediate position and hangs the post 11 and be connected. The cover plate 8 is 2mm in thickness and 125mm in diameter, is provided with an air hole which is aligned with the object placing hole 5 and is 5mm in diameter, and a square hole with the side length of 10mm is arranged in the middle of the cover plate 8 so as to be conveniently installed on the suspension column 11. The hanging column 11 is a regular quadrangular prism with the side length of 9mm and the height of 155mm, prevents the cover plate 8 from rotating, the lower bottom surface is connected with the object placing plate 2, and the upper bottom surface is connected with a bolt column with the diameter of 9mm and the height of 8 mm. The cross support frames 10 are 8mm thick, a bolt hole with the diameter of 9mm is designed in the middle of the cross support frames to be connected with the suspension columns 11, and the length and the width of the two cross support frames 10 are equal and are respectively 150mm and 20 mm.
The experimental solvent container 1 was still used with a 2000mL beaker, and the uncovered cylindrical container 3 was 80mm high, 22mm in outer diameter of the lower bottom surface, 1.5mm in thickness, and divided into upper and lower portions by the protruding ring 6. The uncovered cylindrical container 3 is designed with a square frosted writing area 7 with the length of 15mm, the width of 15mm, the distance between the upper edge of the container and the upper edge of the container is 5mm, and the distance between the lower edge of the container and the upper edge of the protruding ring 6 is 18 mm. The width of the protruding ring 6 is 2mm, the protruding height is 3mm, the upper edge is 38mm away from the upper edge of the container,the lower edge is 40mm away from the lower bottom surface of the container. The lower half part and the lower bottom surface of the uncovered cylindrical container 3 are uniformly distributed with the pore diameter of 0.1mm and the density of 250/cm2And water permeable holes 4. The container suspension bracket is composed of a suspension column 11 and a cross-shaped support frame 10, and the object placing plate 2 and the cover plate 8 are arranged on the suspension column 11. Wherein put the thing board 2 and hang the one end of post 11 and be connected, put thing board 2 thickness and be 2mm, the diameter is 125mm, goes up to have the diameter to put 518 thing holes for 23mm, puts 2 intermediate positions of thing board and hangs the post 11 and be connected. The cover plate 8 is 3mm thick, 125mm in diameter, is provided with a vent hole which is aligned with the object placing hole 5 and is 10mm in diameter, and a square hole with the side length of 10mm is arranged in the middle of the cover plate 8 so as to be conveniently installed on the suspension column 11. The hanging column 11 is a regular quadrangular prism with the side length of 9mm and the height of 155mm, prevents the cover plate 8 from rotating, and the lower bottom surface is connected with the object placing plate 2 (12). The upper bottom surface is connected with a bolt column with the diameter of 9mm and the height of 10 mm. The thickness of the cross-shaped support frames is 10mm, a bolt hole with the diameter of 9mm is designed in the middle of the cross-shaped support frames to be connected with the suspension columns 11, and the length and the width of the two cross-shaped support frames 10 are equal and are respectively 150mm and 15 mm.
A1000 mL beaker was used as the experimental solvent container 1, and a lidded cylindrical container 3 having a height of 50mm, an outer diameter of the lower bottom surface of 19mm and a thickness of 1.5mm was divided into upper and lower portions by a projecting ring 6. The uncovered cylindrical container 3 is designed with a square frosted writing area 7 with a length of 10mm and a width of 10mm, the upper edge of the square frosted writing area is 5mm away from the upper edge of the container, and the lower edge of the square frosted writing area is 4mm away from the upper edge of the protruding ring 6 (3). The width of the protruding ring 6 is 2mm, the protruding height is 4mm, the upper edge is 18mm away from the upper edge of the container, and the lower edge is 30mm away from the lower bottom surface of the container. The lower half part and the lower bottom surface of the uncovered cylindrical container 3 are uniformly distributed with the pore diameter of 0.15mm and the density of 200/cm2And water permeable holes 4. The container suspension bracket is composed of a suspension column 11 and a cross-shaped support frame 10, and the object placing plate 2 and the cover plate 8 are arranged on the suspension column 11. Wherein put the thing board 2 and hang the one end of post 11 and be connected, put thing board 2 thickness and be 3mm, the diameter is 99mm, goes up to have the diameter to put thing hole 518 for 20mm, puts 2 intermediate positions of thing board and hangs the post 11 and be connected. The cover plate 8 is 2mm in thickness and 99mm in diameter, is provided with an air hole which is aligned with the object placing hole 5 and is 5mm in diameter, and a square hole with the side length of 15mm is arranged in the middle of the cover plate 8 so as to be conveniently installed on the suspension column 11. The suspension column 11 is a regular quadrangular prism with the side length of 14mm and the height of 120mm and is used for preventingThe stop cover plate 8 rotates, the lower bottom surface is connected with the object placing plate 2, and the upper bottom surface is connected with a bolt column with the diameter of 15mm and the height of 8 mm. The cross support frames 10 are 8mm thick, bolt holes with the diameter of 15mm are designed in the middle of the cross support frames to be connected with the suspension columns 11, and the length and the width of the two cross support frames 10 are equal and are respectively 120mm and 10 mm.
It should be noted that, in this embodiment, the manufacturing material of the whole device is made of a thermal stable material with a temperature of 100 ℃ or higher, so that the stability of the whole device is ensured and the device is not deformed by heating during the experiment.
According to the embodiment, when the plant mycorrhiza infection rate is detected, the container hanging bracket is assembled, the cover plate 8 is arranged on the hanging column 11, and the object placing plate 2 is fixedly connected with the hanging column 11, so that the object placing holes 5 correspond to the air holes. After the root system is placed into the uncovered cylindrical container 3, the cover plate 8 is lifted, the container is placed into the object placing hole 5 on the object placing plate 2, the cover plate 8 with the hole is put down, the whole container is placed into the experimental solvent container 1 containing the reagent, and the whole device is lifted out when the container is taken out. Because the hole 4 of permeating water of uncovered cylindrical container 3 can effectively guarantee that the reagent infiltration flows out, and does not lose the plant roots, improves sample treatment efficiency, save time. The container suspension bracket keeps the uncovered cylindrical container 3 from toppling over on the basis of ensuring ventilation, so that the sample is conveniently taken and placed. The water permeable holes at the bottom of the uncovered cylindrical container are fully utilized, the heat source is relatively kept away, and the integrity of the root system is ensured.
Corresponding to the apparatus for conveniently and rapidly detecting the mycorrhiza infection rate of plants in batches provided by the above embodiment, the embodiment of the present application further provides a method for using the apparatus for conveniently and rapidly detecting the mycorrhiza infection rate of plants in batches, referring to fig. 6, the method includes:
and S101, assembling the container hanging bracket, the object placing plate 2 and the cover plate 8 according to the object placing holes 5 and the air holes correspondingly.
S102, putting the plant roots into the uncovered cylindrical container 3, then lifting the cover plate 8, putting the uncovered cylindrical container 3 with the plant roots into the object placing hole 5 on the object placing plate 2, and putting down the cover plate 8.
S103, placing the cylindrical container with the cover into the experimental solvent container 1 containing the experimental solvent through the container hanging bracket to carry out heating operation.
And S104, taking out the cylindrical container with the cover through the container hanging bracket after heating is finished.
It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Of course, the above description is not limited to the above examples, and technical features that are not described in this application may be implemented by or using the prior art, and are not described herein again; the above embodiments and drawings are only for illustrating the technical solutions of the present application and not for limiting the present application, and the present application is only described in detail with reference to the preferred embodiments instead, it should be understood by those skilled in the art that changes, modifications, additions or substitutions within the spirit and scope of the present application may be made by those skilled in the art without departing from the spirit of the present application, and the scope of the claims of the present application should also be covered.
Claims (10)
1. The utility model provides a device that convenient batch inspection plant mycorrhiza infects rate which characterized in that includes: the container hanging bracket is arranged at the end part of the experimental solvent container, the first end of the container hanging bracket is movably arranged at the end part of the experimental solvent container, and the second end of the container hanging bracket extends into the experimental solvent container; the second end of the container hanging bracket is connected with an object placing plate, and a gap is formed between the edge of the object placing plate and the inner wall of the experimental solvent container; the experiment solvent container is characterized in that a uncovered cylindrical container is arranged on the object placing plate, a water permeable hole is formed in the part, located between the object placing plate and the bottom of the experiment solvent container, of the uncovered cylindrical container, and a gap exists between the bottom of the uncovered cylindrical container and the bottom of the experiment solvent container.
2. The device for conveniently and massively detecting the mycorrhiza infestation rate of plants according to claim 1, wherein 6N object placing holes are formed in the object placing plate, N is a positive integer greater than 1, and the uncovered cylindrical container is arranged in the object placing holes.
3. The device for conveniently and massively detecting the mycorrhiza infestation rate of plants according to claim 2, wherein a protruding ring is arranged on the uncovered cylindrical container, and the diameter of the protruding ring is larger than that of the object placing hole.
4. The device for conveniently and massively detecting the infection rate of the plant mycorrhiza according to claim 3, wherein a cover plate is further arranged on the container suspension bracket, air outlet holes are formed in the cover plate, the number of the air outlet holes is the same as that of the storage holes, the air outlet holes are correspondingly arranged, and a gap exists between the edge of the cover plate and the inner wall of the experimental solvent container.
5. The device for conveniently and massively detecting the mycorrhiza infection rate of plants according to claim 4, wherein the container hanging bracket comprises a cross-shaped supporting frame and a hanging column, one end of the hanging column is movably connected with the cross-shaped supporting frame, the other end of the hanging column is movably connected with the object placing plate, and the cover plate is movably arranged on the hanging column between the cross-shaped supporting frame and the object placing plate.
6. The device for conveniently and massively detecting the infection rate of the plant mycorrhiza according to claim 5, wherein a first screw hole is formed in the cross-shaped support frame, a second screw hole is formed in the object placing plate, external threads corresponding to the first screw hole and the second screw hole are respectively formed in two ends of the suspension column, and the suspension column is respectively in threaded connection with the cross-shaped support frame and the object placing plate.
7. The device for conveniently and massively detecting the mycorrhiza infestation rate of plants according to claim 6, wherein the cross section of the hanging column main body is non-circular or non-quasi-circular, the center of the cover plate is provided with a non-circular hole or a non-quasi-circular hole corresponding to the cross section of the hanging column main body, and the cover plate is movably connected with the hanging column.
8. The device for conveniently and massively detecting the mycorrhiza infestation rate of plants according to any one of claims 1 to 7, wherein the uncovered cylindrical container is provided with a frosted writing area.
9. The apparatus for conveniently and rapidly detecting the mycorrhiza infection rate of plants in batch according to claim 7, wherein the gap between the bottom of the uncovered cylindrical container and the bottom of the experimental solvent container is 3-5mm, and the gap between the edges of the object placing plate and the cover plate and the inner wall of the experimental solvent container is 1-3 mm.
10. A method of using a device for facilitating batch testing of mycorrhiza infestation rates of plants, wherein the device of any one of claims 1-9 is used, the method comprising:
assembling the container hanging bracket, the object placing plate and the cover plate according to the object placing holes and the air holes correspondingly;
putting the plant root system into the uncovered cylindrical container, then lifting the cover plate, putting the uncovered cylindrical container with the plant root system into the storage hole on the storage plate, and putting down the cover plate;
placing the cylindrical container with the cover into an experimental solvent container containing an experimental solvent through a container hanging bracket to carry out heating operation;
and taking out the cylindrical container with the cover through the container hanging bracket after heating.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113853873A (en) * | 2021-11-15 | 2021-12-31 | 山东农业大学 | Portable small-particle seed soaking device and use method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002255715A (en) * | 2001-02-23 | 2002-09-11 | Yamaki Co Ltd | Method for producing mycorrhizal fungus growth- promoting agent |
| JP2009296977A (en) * | 2008-06-17 | 2009-12-24 | Hokken Co Ltd | Method for producing japanese red pine young seedling infected with mycorrhizal fungus |
| CN108293504A (en) * | 2018-01-17 | 2018-07-20 | 广西壮族自治区中国科学院广西植物研究所 | A kind of device for raising seedlings and method for cultivating mycorrhizal seedlings |
| CN208013092U (en) * | 2018-01-25 | 2018-10-26 | 中国热带农业科学院热带作物品种资源研究所 | A kind of device of quick measurement plant Mycorrhizal Infection Incidence |
| CN208875040U (en) * | 2018-10-15 | 2019-05-21 | 中国烟草总公司郑州烟草研究院 | A kind of vegetable seeds or seedling hydroponic device |
| CN110521564A (en) * | 2019-09-17 | 2019-12-03 | 华南农业大学 | Living body mycelia net infects the mycorrhizal seedling raising method of plant |
-
2020
- 2020-11-18 CN CN202011289798.8A patent/CN112470750B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002255715A (en) * | 2001-02-23 | 2002-09-11 | Yamaki Co Ltd | Method for producing mycorrhizal fungus growth- promoting agent |
| JP2009296977A (en) * | 2008-06-17 | 2009-12-24 | Hokken Co Ltd | Method for producing japanese red pine young seedling infected with mycorrhizal fungus |
| CN108293504A (en) * | 2018-01-17 | 2018-07-20 | 广西壮族自治区中国科学院广西植物研究所 | A kind of device for raising seedlings and method for cultivating mycorrhizal seedlings |
| CN208013092U (en) * | 2018-01-25 | 2018-10-26 | 中国热带农业科学院热带作物品种资源研究所 | A kind of device of quick measurement plant Mycorrhizal Infection Incidence |
| CN208875040U (en) * | 2018-10-15 | 2019-05-21 | 中国烟草总公司郑州烟草研究院 | A kind of vegetable seeds or seedling hydroponic device |
| CN110521564A (en) * | 2019-09-17 | 2019-12-03 | 华南农业大学 | Living body mycelia net infects the mycorrhizal seedling raising method of plant |
Non-Patent Citations (1)
| Title |
|---|
| 王港等: "华北山地6个外生菌根树种的根属性及其与菌根侵染率的关系", 《西北植物学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113853873A (en) * | 2021-11-15 | 2021-12-31 | 山东农业大学 | Portable small-particle seed soaking device and use method thereof |
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