CN105123301A - Method for predicting invasion potential of soil tobacco black shank - Google Patents
Method for predicting invasion potential of soil tobacco black shank Download PDFInfo
- Publication number
- CN105123301A CN105123301A CN201510635459.3A CN201510635459A CN105123301A CN 105123301 A CN105123301 A CN 105123301A CN 201510635459 A CN201510635459 A CN 201510635459A CN 105123301 A CN105123301 A CN 105123301A
- Authority
- CN
- China
- Prior art keywords
- soil
- disease
- tobacco
- incidence
- forecasting methodology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Environmental Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for predicting the invasion potential of soil tobacco black shank. The prediction method comprises the following steps: performing five-point sampling to excavate soil samples, removing impurities, crushing the samples, and keeping the samples wet; culturing tobaccos into seedlings with 5-6 true leaves according to a conventional floating seedling raising method under an indication, shearing tobacco seedling root soil, transplanting the seedlings into a screen basket containing the soil samples, keeping the soil wet, and culturing the seedlings in an environment with alternating illumination and darkness at an interval of 12 hours, wherein the temperature is 25-28 DEG C and the relative humidity is more than or equal to 75%; and observing the condition of invasion on the second and third days after temporary planting, investigating once every 6-8 days for at least 5 times, recording an invasion if obvious disease spots are generated until the tobacco seedlings are withered, recording no invasion if no disease spot is generated and the tobacco seedlings are straight and grow normally, recording the state of diseases according to the incidence rate, and predicting the invasion potential according to a field disease prevalent law. The prediction method can be used for scientifically predicting the invasion potential of the soil black shank to lay a foundation for layout of varieties with different resistance, field screening of disease nurseries and reduction of invasion risks, so that the prediction method is suitable for large-scale popularization and application.
Description
Technical field
The invention belongs to tobacco-growing soil morbidity Potential Prediction technical field, be specifically related to the Forecasting Methodology of a kind of soil black shank morbidity potentiality.
Background technology
Black shank be by Phytophthora nicotianae (
phytophthoranicotianae) soil-borne disease that causes, under natural conditions, so that plant residue in soil of mycelium, egg spore and chlamydospore to be survived the winter.Intensive seedling production, can control sprout term disease substantially, and land for growing field crops is then the main place of disease control.At present, the method being separated qualification and counting Phytophthora nicotianae Breda from soil has more report.As blade bait method and soil dilution planting, (in the soil such as Luo Wenfu, Phytophthora nicotianae is separated and quantitative determination Chinese tobacco journal, 1999,5 (4): 29-31), (in a kind of soil such as Wang Han, the quick separating of Phytophthora nicotianae Breda is identified and method of counting China agronomy circular for soil dilution planting and PCR proof method, 2012,28 (9): 163-168), fluorescent quantitative PCR technique (Chinese invention patent application CN201110402300.9; The foundation of black shank bacterium quantitative fluorescent PCR rapid detection system and Preliminary Applications Agricultural University Of Jiangxi journal in the soil such as Pan Mingsen, 2015,37 (4): 712-718) method such as.Blade bait method can detect the phytophthora existed with zoospore form, carries out relative quantification; Soil dilution planting can obtain live strain, the phytophthora in quantitative soil, adds that PCR checking can be more effective, but can not distinguish the form of phytophthora existence; Fluorescence quantifying PCR method can be identified phytophthora quickly and count, but cannot distinguish dead cell and living cells, and technical difficulty is large, cost is high, can not promote in forefront of the production.What forefront of the production used remains based on the method for China's tobacco industry standard YC/T341, but the method time span is large, relate to whole production season, and predict in conjunction with meteorological condition mainly through incidence over the years, do not relate to the quantity of Phytophthora nicotianae Breda.Therefore, necessary set up a kind of relatively accurate, be simple and easy to promote, cost is low, be applicable to the black shank Forecasting Methodology of forefront of the production application.
Summary of the invention
The object of the present invention is to provide the Forecasting Methodology of a kind of soil black shank morbidity potentiality.
The object of the present invention is achieved like this: the Forecasting Methodology of described soil black shank morbidity potentiality, comprise the Acquire and process of soil specimen, instruction tobacco heel in cultivation and disease survey and Prediction of Incidence Trend operation, specifically comprise:
The Acquire and process of A, soil specimen: in the plot of preparation plantation tobacco, 5 samplings take soil specimen, depth selection 15 ~ 20cm, often some sampling 1.0 ~ 1.5kg, merge, mix, impurities removing, rolles over and is broken into soil particle, loads in sieve basket, sieve basket is placed in pallet, in pallet, inject water, moistening soil, adhesion damp with soil is advisable;
B, instruction tobacco heel in cultivation: instruction tobacco routinely floating seedlings mode cultivate to 5 ~ 6 true leaf seedlings, select the cigarette seedling that growing way is neat, shear cigarette shoot root soil, transplant in the sieve basket that soil specimen is housed, the sieve basket heeling in cigarette seedling is placed in water tray, maintains ground moistening, at 25 ~ 28 DEG C, relative moisture more than 75%, cultivates in the phytotron that 12 h light 12 h dark replace;
C, disease survey and Prediction of Incidence Trend: after heeling in, within 2nd ~ 3 days, observe incidence, investigation in every 6 ~ 8 days 1 state of an illness, be no less than 5 times, black shank is investigated in units of strain, be denoted as morbidity to produce obvious scab to cigarette seedling is wilting, be normally denoted as do not fall ill with, growth upright without scab, cigarette seedling, investigate all cigarette strains, with the incidence of disease record state of an illness, according to field diseases regularty of epidemic prediction morbidity potentiality.
Forecasting Methodology of the present invention is owing to adopting the seedling of cultivars to disease susceptible as indicator plant, root is hindered by shearing, heel in the moistening cultivation of pallet, effectively field natural occurrence can be simulated under human controllable's environmental condition of temperature control, moisturizing, illumination relative constancy, the disease survey of one month in conjunction with the morbidity potentiality of the measurable soil balck shank of field diseases regularty of epidemic, for different resistant variety layout, the sick garden of field examination disease, reduce onset risk and lay the foundation.Forecasting process is easy to learn, programmable, and accurately and reliably, quickness and high efficiency, can be applicable on a large scale in tobacco planting region.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
The Forecasting Methodology of soil black shank of the present invention morbidity potentiality, comprise the Acquire and process of soil specimen, instruction tobacco heel in cultivation and disease survey and Prediction of Incidence Trend operation, specifically comprise:
The Acquire and process operation of described soil specimen refers to: in the plot of preparation plantation tobacco, 5 samplings take soil specimen, depth selection 15 ~ 20cm, often some sampling 1.0 ~ 1.5kg, merge, mix, impurities removing, rolles over and is broken into soil particle, loads in sieve basket, sieve basket is placed in pallet, in pallet, inject water, moistening soil, adhesion damp with soil is advisable;
The cultivation operation of heeling in of described instruction tobacco refers to: instruction tobacco routinely floating seedlings mode is cultivated to 5 ~ 6 true leaf seedlings, select the cigarette seedling that growing way is neat, shear cigarette shoot root soil, transplant in the sieve basket that soil specimen is housed, the sieve basket heeling in cigarette seedling is placed in water tray, maintains ground moistening, at 25 ~ 28 DEG C, relative moisture more than 75%, cultivates in the phytotron that 12 h light 12 h dark replace;
Described disease survey and Prediction of Incidence Trend operation refer to: within 2nd ~ 3 days, observe incidence after heeling in, investigation in every 6 ~ 8 days 1 state of an illness, be no less than 5 times, black shank is investigated in units of strain, be denoted as morbidity to produce obvious scab to cigarette seedling is wilting, be normally denoted as do not fall ill with, growth upright without scab, cigarette seedling, investigate all cigarette strains, with the incidence of disease record state of an illness, according to field diseases regularty of epidemic prediction morbidity potentiality.
The particle diameter of soil particle described in steps A is 0.5 ~ 1.0cm.
In pallet, inject water described in steps A, moistening soil, in pallet, the degree of depth of water is 1/3 ~ 1/2 of soil height in sieve basket.
The time of moistening soil described in steps A is 2 ~ 3 days.
Indicate tobacco to refer to easily susceptible tobacco bred described in step B, can be the large gold dollar of safflower, dark green No. 1, KRK26, any one in white rib 21 or little gold 1025.
Select the cigarette seedling that growing way is neat described in step B, during transplanting is interior to the sieve basket that soil specimen is housed, the cigarette shoot root soil of 1/4 ~ 1/3 need be sheared.
The cigarette seedling that growing way is neat is selected described in step B, during transplanting is interior to the sieve basket that soil specimen is housed, the strain of every basket of cigarette seedling number at least 24.
The cigarette seedling heeled in described in step B is placed in water tray, maintains ground moistening, and in pallet, the degree of depth of water is 1/3 ~ 1/2 of soil height in sieve basket.
Water described in steps A, B can be any one in running water, pure water or distilled water.
Disease survey described in step C and Prediction of Incidence Trend observe incidence in the 3rd day after heeling in, investigation in every 7 days 1 state of an illness, totally 5 times.
Refer to according to field diseases regularty of epidemic according to field diseases regularty of epidemic prediction morbidity potentiality described in step C, the initial incidence of disease more than 5%, not carrying out preventing and treating, temp. and humidity is suitable, when not removing diseased plant, the morbidity Sheng phase in theory the incidence of disease can reach 5 ~ 8 times.
embodiment
---the prediction of 10 soil specimen black shank generation potentiality
The present embodiment gets 10 soil specimens altogether, adopts the large gold dollar of cultivars to disease susceptible safflower (HD) as instruction tobacco.
Concrete operations are as follows:
The Acquire and process of A, soil specimen: in the plot of preparation plantation tobacco, 5 samplings take soil specimen, depth selection 18cm, often some sampling 1.2kg, merge, mix, impurities removing, roll over the soil particle being broken into particle diameter 0.5 ~ 1.0cm, load in sieve basket, sieve basket is placed in pallet, in pallet, injects running water, make the degree of depth of water in pallet be 1/3 of soil height in sieve basket, moistening soil 2 days, adhesion damp with soil is advisable.
B, instruction tobacco heel in cultivation: instruction tobacco safflower large gold dollar routinely floating seedlings mode is cultivated to 5 ~ 6 true leaf seedlings, select the cigarette seedling that growing way is neat, shear the cigarette shoot root soil of 1/3, transplant in the sieve basket that soil specimen is housed, the strain of every basket of cigarette seedling number 24, the sieve basket heeling in cigarette seedling is placed in the pallet containing running water, in pallet, the degree of depth of water is 1/2 of soil height in sieve basket, maintain ground moistening, at 25 ~ 28 DEG C, relative moisture more than 75%, cultivates in the phytotron that 12 h light 12 h dark replace.
C, disease survey and Prediction of Incidence Trend: after heeling in, within the 3rd day, observe incidence, investigation in every 7 days 1 state of an illness, totally 5 times, black shank is investigated in units of strain, be denoted as morbidity to produce obvious scab to cigarette seedling is wilting, be normally denoted as do not fall ill with, growth upright without scab, cigarette seedling, investigate all cigarette strains, with the incidence of disease record state of an illness, according to field diseases regularty of epidemic prediction morbidity potentiality.
Experimental result is in table 1, experimental result shows, 10 soil specimen morbidity potentiality differences are obvious, put off in time, the incidence of disease rises to some extent, and the sample of always not falling ill has 2, and the sample of the incidence of disease < 10% of last 1 investigation has 1, the sample of 10%≤incidence of disease < 20.0% has 4, and the sample of the incidence of disease >=20% has 3.According to field diseases regularty of epidemic, the initial incidence of disease more than 5%, not carrying out preventing and treating, temp. and humidity is suitable, when not removing diseased plant, the morbidity Sheng phase in theory the incidence of disease can reach 5 ~ 8 times.Therefore, plant in the soil that cultivars to disease susceptible only can not fallen ill at 2, the soil of the incidence of disease >=20% of last 1 investigation then can not plant cultivars to disease susceptible, the soil of 10%≤incidence of disease < 20.0%, needs the prevention and control strengthening disease when planting cultivars to disease susceptible.
Table 1 soil specimen black shank incidence is added up
Claims (10)
1. a Forecasting Methodology for soil black shank morbidity potentiality, it is characterized in that comprising the Acquire and process of soil specimen, instruction tobacco heel in cultivation and disease survey and Prediction of Incidence Trend operation, specifically comprise:
The Acquire and process of A, soil specimen: in the plot of preparation plantation tobacco, 5 samplings take soil specimen, depth selection 15 ~ 20cm, often some sampling 1.0 ~ 1.5kg, merge, mix, impurities removing, rolles over and is broken into soil particle, loads in sieve basket, sieve basket is placed in pallet, in pallet, inject water, moistening soil, adhesion damp with soil is advisable;
B, instruction tobacco heel in cultivation: instruction tobacco routinely floating seedlings mode cultivate to 5 ~ 6 true leaf seedlings, select the cigarette seedling that growing way is neat, shear cigarette shoot root soil, transplant in the sieve basket that soil specimen is housed, the sieve basket heeling in cigarette seedling is placed in water tray, maintains ground moistening, at 25 ~ 28 DEG C, relative moisture more than 75%, cultivates in the phytotron that 12 h light 12 h dark replace;
C, disease survey and Prediction of Incidence Trend: after heeling in, within 2nd ~ 3 days, observe incidence, investigation in every 6 ~ 8 days 1 state of an illness, be no less than 5 times, black shank is investigated in units of strain, be denoted as morbidity to produce obvious scab to cigarette seedling is wilting, be normally denoted as do not fall ill with, growth upright without scab, cigarette seedling, investigate all cigarette strains, with the incidence of disease record state of an illness, according to field diseases regularty of epidemic prediction morbidity potentiality.
2. Forecasting Methodology according to claim 1, is characterized in that the particle diameter of soil particle described in steps A is 0.5 ~ 1.0cm.
3. Forecasting Methodology according to claim 1, is characterized in that in pallet, injecting water described in steps A, moistening soil, and in pallet, the degree of depth of water is 1/3 ~ 1/2 of soil height in sieve basket.
4. Forecasting Methodology according to claim 1, is characterized in that the time of moistening soil described in steps A is 2 ~ 3 days.
5. Forecasting Methodology according to claim 1, it is characterized in that indicating described in step B tobacco can be the large gold dollar of safflower, dark green No. 1, KRK26, any one in white rib 21 or little gold 1025.
6. Forecasting Methodology according to claim 1, is characterized in that selecting the neat cigarette seedling of growing way described in step B, during transplanting is interior to the sieve basket that soil specimen is housed, need shear the cigarette shoot root soil of 1/4 ~ 1/3.
7. Forecasting Methodology according to claim 1, is characterized in that selecting the neat cigarette seedling of growing way described in step B, during transplanting is interior to the sieve basket that soil specimen is housed, and the strain of every basket of cigarette seedling number at least 24.
8. Forecasting Methodology according to claim 1, is characterized in that the cigarette seedling heeled in described in step B is placed in water tray, maintains ground moistening, and in pallet, the degree of depth of water is 1/3 ~ 1/2 of soil height in sieve basket.
9. Forecasting Methodology according to claim 1, is characterized in that disease survey described in step C and Prediction of Incidence Trend, is to observe incidence in the 3rd day after heeling in, investigation in every 7 days 1 state of an illness, totally 5 times.
10. Forecasting Methodology according to claim 1, it is characterized in that referring to according to field diseases regularty of epidemic according to field diseases regularty of epidemic prediction morbidity potentiality described in step C, the initial incidence of disease is more than 5%, not carrying out preventing and treating, temp. and humidity is suitable, when not removing diseased plant, the morbidity Sheng phase in theory the incidence of disease can reach 5 ~ 8 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510635459.3A CN105123301B (en) | 2015-09-30 | 2015-09-30 | A kind of Forecasting Methodology of soil tobacco black shank morbidity potentiality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510635459.3A CN105123301B (en) | 2015-09-30 | 2015-09-30 | A kind of Forecasting Methodology of soil tobacco black shank morbidity potentiality |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105123301A true CN105123301A (en) | 2015-12-09 |
| CN105123301B CN105123301B (en) | 2017-09-12 |
Family
ID=54709199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510635459.3A Active CN105123301B (en) | 2015-09-30 | 2015-09-30 | A kind of Forecasting Methodology of soil tobacco black shank morbidity potentiality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105123301B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110036860A (en) * | 2019-04-26 | 2019-07-23 | 中国烟草总公司郑州烟草研究院 | Method for judging tobacco root Disease pressure |
| CN111727783A (en) * | 2020-07-27 | 2020-10-02 | 贵州省烟草科学研究院 | Method for forecasting starting period of alternaria alternata |
| CN113966704A (en) * | 2021-10-27 | 2022-01-25 | 云南省烟草公司大理州公司 | Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491199A (en) * | 2009-03-09 | 2009-07-29 | 云南省烟草科学研究所 | Tobacco black shank resistance disease nursery identification method |
| CN101999287A (en) * | 2010-09-14 | 2011-04-06 | 云南省烟草农业科学研究院 | Thermostatic hydroponic identification method for resistance of tobacco black shank at seedling stage |
| CN102498878A (en) * | 2011-11-03 | 2012-06-20 | 云南省烟草农业科学研究院 | New method for identifying resistance in black shank quickly |
| CN104745672A (en) * | 2015-03-04 | 2015-07-01 | 河南省农业科学院烟草研究所 | Method for rapidly identifying black shank resistance of tobaccos |
-
2015
- 2015-09-30 CN CN201510635459.3A patent/CN105123301B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491199A (en) * | 2009-03-09 | 2009-07-29 | 云南省烟草科学研究所 | Tobacco black shank resistance disease nursery identification method |
| CN101999287A (en) * | 2010-09-14 | 2011-04-06 | 云南省烟草农业科学研究院 | Thermostatic hydroponic identification method for resistance of tobacco black shank at seedling stage |
| CN102498878A (en) * | 2011-11-03 | 2012-06-20 | 云南省烟草农业科学研究院 | New method for identifying resistance in black shank quickly |
| CN104745672A (en) * | 2015-03-04 | 2015-07-01 | 河南省农业科学院烟草研究所 | Method for rapidly identifying black shank resistance of tobaccos |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110036860A (en) * | 2019-04-26 | 2019-07-23 | 中国烟草总公司郑州烟草研究院 | Method for judging tobacco root Disease pressure |
| CN111727783A (en) * | 2020-07-27 | 2020-10-02 | 贵州省烟草科学研究院 | Method for forecasting starting period of alternaria alternata |
| CN113966704A (en) * | 2021-10-27 | 2022-01-25 | 云南省烟草公司大理州公司 | Black shank induction morbidity method for potted tobacco leaves of Honghuadajinyuan variety |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105123301B (en) | 2017-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wu et al. | Enhancing rapeseed tolerance to heat and drought stresses in a changing climate: perspectives for stress adaptation from root system architecture | |
| Klimešová et al. | Handbook of standardized protocols for collecting plant modularity traits | |
| Novozhilov et al. | Ecology and distribution of myxomycetes | |
| Brodde et al. | Diplodia tip blight on its way to the north: drivers of disease emergence in northern Europe | |
| Perkons et al. | Root-length densities of various annual crops following crops with contrasting root systems | |
| Zeng et al. | Changes in soil organic carbon, nitrogen, phosphorus, and bulk density after afforestation of the “Beijing–Tianjin Sandstorm Source Control” program in China | |
| Xia et al. | Epichloë endophyte affects the ability of powdery mildew (Blumeria graminis) to colonise drunken horse grass (Achnatherum inebrians) | |
| Leuschner et al. | Large altitudinal increase in tree root/shoot ratio in tropical mountain forests of Ecuador | |
| Carvalho et al. | Input of sugarcane post-harvest residues into the soil | |
| Silva et al. | Relationships between physiological traits and productivity of sugarcane in response to water deficit | |
| de Oliveira et al. | Least limiting water range as influenced by tillage and cover crop | |
| Du et al. | Influence of no-tillage and precipitation pulse on continuous soil respiration of summer maize affected by soil water in the North China Plain | |
| Khattak et al. | Allelopathic potential of Jatropha curcas L. leaf aqueous extracts on seedling growth of wheat | |
| Pitre et al. | Estimating root biomass in Salix viminalis× Salix schwerinii cultivar “Olof” using the electrical capacitance method | |
| CN105123301A (en) | Method for predicting invasion potential of soil tobacco black shank | |
| Fitters et al. | Assessing water uptake in sugar beet (Beta vulgaris) under different watering regimes | |
| Rubilar et al. | Influences of silvicultural manipulations on above-and belowground biomass accumulations and leaf area in young Pinus radiata plantations, at three contrasting sites in Chile | |
| Faget et al. | Root growth of maize in an Italian ryegrass living mulch studied with a non-destructive method | |
| Sukhomlin et al. | The current state of the population of the golden potato cyst nematode Globodera rostochiensis (Nematoda, Heteroderidae) in the northwest of Ukraine | |
| CN102511368A (en) | Method for evaluating resistance of brassica plants on club root | |
| Freitas et al. | Terracing increases soil available water to plants in no-tillage | |
| CN103460933A (en) | Treatment method of seeds for planting and breeding of polygonum capitatum of Relinqing particle medicinal materials | |
| Song et al. | Defoliation and neighbouring legume plants accelerate leaf and root litter decomposition of Leymus chinensis dominating grasslands | |
| Cerqueira et al. | Competition between drought-tolerant upland rice cultivars and weeds under water stress condition | |
| Lievens | Parameterization and testing of the FAO Aquacrop model to simulate yield response to water in North-eastern Thailand |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |