CN110089379A - For identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery - Google Patents
For identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery Download PDFInfo
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- CN110089379A CN110089379A CN201910293020.5A CN201910293020A CN110089379A CN 110089379 A CN110089379 A CN 110089379A CN 201910293020 A CN201910293020 A CN 201910293020A CN 110089379 A CN110089379 A CN 110089379A
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- black rot
- cigarette strain
- disease
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- root black
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of for identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery, the flat plot of suitable planting tobacco is selected first, susceptible black rot kind is chosen to be planted, it is inoculated with root black rot germ, when the mature later period, it after taking Field diseased plant stalk to be crushed, is uniformly sprinkling upon in sick nursery plot, then carries out turning over earthing；Second year, susceptible black rot kind anti-kind in is chosen to be inoculated with respectively and be not inoculated with root black rot germ, it is inoculated with identical with nonvaccinated Variety Disease-resistance grade, the plot is used as root black rot sick nursery to carry out root black rot Resistance Identification to tobacco bred and uses, grade is different, continue to fall ill and uniformly spread after cigarette strain stalk is crushed to sick nursery plot and carry out turning over earthing, field plot test is identical to grade again.Long the time required to establishing the present invention overcomes sick nursery, plot selection such as requires more than high and unstable factor at the environmental restrictions factor, has the characteristics that quickly to establish accuracy high, repeated strong.
Description
Technical field
The present invention relates to tobacco disease resistance breeding fields, and in particular to one kind is for identifying burley tobaccos kind root black rot resistance
The method for building up of sick nursery.
Background technique
It usually requires to establish stable and accurate method of resistance identification during breeding for disease resistance, to select to resist
Sick breeding material, establishing sick nursery is the important channel that traditional tobacco breeding carries out Disease Resistance Identification, by tobacco breeding material kind
It is implanted in the sick nursery under grown in field environment, is traditional tobacco breeding for disease resistance in conjunction with artificial infection and spontaneous induction morbidity identification
The main method of use.
Currently, the sick nursery foundation for Resistance Identification is the most important thing of accurate breeding for disease resistance.Usual field sick nursery is built
The serious multiple plot of vertical more options disease, it is this meet establish specification sick nursery plot it is not only few, but also since disease is multiple
Plot disease species it is more, germ content type is more in soil and single germ amount is insufficient, on the identification of single disease influence compared with
Greatly, for this purpose, on the plot of the suitable planting tobacco of block one by one in office, the single germ amount of artificial increase in field, and then fast run-up
The root black rot Resistance Identification sick nursery that vertical accuracy is high, repeatability is strong.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of for identifying the black corruption of burley tobaccos kind root
The method for building up of sick resistance sick nursery, this method have the characteristics that accuracy is high, repeatability is strong.
To achieve the above object, a kind of for identifying building for burley tobaccos kind root black rot resistance sick nursery designed by the present invention
Cube method, comprising the following steps:
1) the flat plot for selecting suitable planting tobacco is chosen susceptible black rot kind and is planted；
2) when cigarette strain grows to the prosperous growth phase, start to be inoculated with root black rot germ in cigarette strain stem；It is grown into cigarette strain
When the ripe later period (when as the morbidity of cigarette strain root black rot reaches peak period), after taking Field diseased plant stalk to be crushed, uniformly it is sprinkling upon
In sick nursery plot, then carry out turning over earthing；
3) second year, choose susceptible black rot kind and/or in anti-kind planted；When cigarette strain grows to the prosperous growth phase
When, when cigarette strain grows to the mature later period (when as the morbidity of cigarette strain root black rot reaches peak period), each kind odd number row or
The cigarette strain stem of even numbers row is inoculated with root black rot germ；
4) cigarette strain of observation inoculation root black rot germ and nonvaccinated cigarette strain incidence, investigate root black rot disease incidence
And sick grade (as unit of strain) of falling ill, disease index is calculated, resistance class is determined according to disease index；
5) cigarette strain of inoculation root black rot germ and nonvaccinated cigarette strain disease index in more same kind:
When inoculation root black rot germ cigarette strain and nonvaccinated cigarette strain disease index in same interval value, that is, judge it
Root black rot state of an illness rank it is consistent, meet known resistance class, continue to fall ill after cigarette strain stalk is crushed and uniformly spread
It to sick nursery plot and carries out turning over earthing, at this point, the plot is used as root black rot sick nursery to carry out root black rot to tobacco bred
Resistance Identification uses；
When inoculation root black rot germ cigarette strain and nonvaccinated cigarette strain not in same interval value, that is, judge that their roots are black
Maize ear rot state of an illness rank is inconsistent, does not meet known disease-resistant grade, continues that diseased plant stalk is taken to be crushed, be uniformly sprinkling upon sick nursery plot
In, then carry out turning over earthing, continue to plant identical susceptible kind and/or middle disease-resistant variety repetitive identified, meets item until meeting
Part can be used as root black rot Resistance Identification sick nursery.
Further, in the step 1), the tobacco bred of susceptible black rot, which is selected from, bar draws No. 2 and Kentucky15.
Still further, in the step 3), in anti-black rot tobacco bred be selected from E'yan 1, HubeiT3 and
Kentucky907。
Still further, root black rot disease incidence and sick grade of falling ill calculate disease index, specific to walk in the step 4)
It is rapid as follows:
A. investigation 1 time before inoculation, investigation in every 5 days is primary after inoculation, investigates 5 times altogether, investigates whole cigarette strain incidences；
B. severity Scaling method:
0 grade: complete stool is disease-free；1 grade: stem's scab is no more than the one third of stem girth；3 grades: stem's scab is around stem girth
One third is to half；5 grades: stem's scab is more than the half of stem girth；7 grades: stem's scab is entirely around stem girth；9
Grade: diseased plant is substantially withered.
C. Evaluation standard of resistance
R (disease-resistant): 20 or less disease index；MR (in resist): disease index 20.1~40；MS (middle sense): disease index
40.1~60；S (susceptible): disease index 60.1~80；HS (susceptible): disease index 80.1~100；
D. to investigate resulting data, the disease index of each kind is calculated as follows:
Beneficial effects of the present invention:
The environmental restrictions such as more than long the time required to the present invention overcomes sick nursery foundation, plot selection requirement height and unstable factor
Factor provides the method for quickly establishing the root black rot Resistance Identification sick nursery that accuracy is high, repeatability is strong.
Specific embodiment
The present invention is described in further detail combined with specific embodiments below, so as to those skilled in the art understand that.
For identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery, comprising the following steps:
1) test sick nursery plot is located at Hubei Province somewhere, for 2 mu of area of examination；Test plot topography is flat, and quality is loose, soil
Earth middle fertility, illumination is sufficient, and drainage is good, suitable planting tobacco；
2) disease-resistant rank is divided into according to disease index: being susceptible when a. disease index is 80.1-100；
B. when disease index is 60.1-80, for sense；It c. is middle sense when disease index is 40.1-60；
D. disease index be 20.1-40 when, be in resist；It e. is anti-when disease index is 0.1-20
No. 2, Kentucky15 are drawn by the upper tobacco bred bar for choosing susceptible black rot of plantation, every two row interval is planted,
Each 1000 plants of plantation；
3) start to be inoculated with root black rot germ in cigarette strain stem at the beginning of 6 months when cigarette strain grows to the prosperous growth phase；It is grown to cigarette strain
When to the mature later period (when as the morbidity of cigarette strain root black rot reaches peak period), after taking Field diseased plant stalk to be crushed, uniformly
It is sprinkling upon in sick nursery plot, then carries out turning over earthing；
4) second year is chosen according to disease-resistant rank and plants susceptible kind cloud and mist 87, Zhongyan-100 and lucky cigarette 9, in anti-kind
E'yan 1, HubeiT3 and Kentucky907 (plantation specification is 120cm × 45cm), when cigarette strain grows to the prosperous growth phase, to cigarette
When strain grows to the mature later period (when as the morbidity of cigarette strain root black rot reaches peak period), each kind odd number row or even numbers row's
Cigarette strain stem is inoculated with root black rot germ；
5) cigarette strain of observation inoculation root black rot germ and nonvaccinated cigarette strain incidence, and investigated after fine day noon
Root black rot disease incidence and sick grade (as unit of strain) of falling ill, calculate disease index；
Specific step is as follows:
A. investigation 1 time before inoculation, investigation in every 5 days is primary after inoculation, investigates 5 times altogether, investigates whole cigarette strain incidences；
B. severity Scaling method:
0 grade: complete stool is disease-free；1 grade: stem's scab is no more than the one third of stem girth；3 grades: stem's scab is around stem girth
One third is to half；5 grades: stem's scab is more than the half of stem girth；7 grades: stem's scab is entirely around stem girth；9
Grade: diseased plant is substantially withered.
C. Evaluation standard of resistance
R (disease-resistant): 20 or less disease index；MR (in resist): disease index 20.1~40；MS (middle sense): disease index
40.1~60；S (susceptible): disease index 60.1~80；HS (susceptible): disease index 80.1~100；
D. to investigate resulting data, the disease index of each kind is calculated as follows:
6) cigarette strain of inoculation root black rot germ and nonvaccinated cigarette strain disease index in more same kind:
1 black rot crop field qualification result of table
As shown in table 1, calculate that susceptible kind bar draws No. 2, Kentucky15 is in inoculation and not by field observation and investigation
The root black rot morbidity disease index and resistance class of inoculation processing, the results showed that each kind disease refers to that value is unanimous on the whole, exists
Within the scope of 80.1-100, disease-resistant grade is susceptible, three in anti-kind E'yan 1, Hubei Province cigarette 3 consistent with previous qualification result
Number and Kentucky907's the result shows that, the inoculation of each kind and be not inoculated with the root black rot morbidity disease index of processing and anti-
Property grade disease refer to that value is unanimous on the whole, disease-resistant grade be in resist, it is consistent with previous qualification result.
Therefore, it uniformly spreads after for the year being crushed the cigarette strain stalk of three susceptible kinds of morbidity to sick nursery plot and goes forward side by side
Row turns over earthing, which is used as root black rot sick nursery to carry out root black rot Resistance Identification to tobacco bred and uses.
Other unspecified parts are the prior art.Although above-described embodiment is made that the present invention and retouches in detail
State, but it is only a part of the embodiment of the present invention, rather than whole embodiments, people can also according to the present embodiment without
Other embodiments are obtained under the premise of creativeness, these embodiments belong to the scope of the present invention.
Claims (4)
1. a kind of for identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery, it is characterised in that: including following step
It is rapid:
1) the flat plot for selecting suitable planting tobacco is chosen susceptible black rot kind and is planted；
2) when cigarette strain grows to the prosperous growth phase, start to be inoculated with root black rot germ in cigarette strain stem；After cigarette strain grows to maturation
It when the phase, after taking Field diseased plant stalk to be crushed, is uniformly sprinkling upon in sick nursery plot, then carries out turning over earthing；
3) second year, choose susceptible black rot kind and/or in anti-kind planted；When cigarette strain grows to the prosperous growth phase,
When cigarette strain grows to the mature later period, the cigarette strain stem of each kind odd number row or even numbers row are inoculated with root black rot germ；
4) cigarette strain of observation inoculation root black rot germ and nonvaccinated cigarette strain incidence, investigate root black rot disease incidence and hair
Sick grade, calculates disease index, determines resistance class according to disease index；5) root black rot germ is inoculated in more same kind
Cigarette strain and nonvaccinated cigarette strain disease index:
When inoculation root black rot germ cigarette strain and nonvaccinated cigarette strain disease index in same interval value, that is, judge their roots
Black rot state of an illness rank is consistent, meet it is known susceptible or in anti-resistance class, after the cigarette strain stalk that continues will to fall ill is crushed
It uniformly spreads to sick nursery plot and carries out turning over earthing, at this point, the plot is used as root black rot sick nursery to carry out root to tobacco bred
Black rot Resistance Identification uses；
When inoculation root black rot germ cigarette strain and nonvaccinated cigarette strain disease index not in same interval value, that is, judge them
Root black rot state of an illness rank is inconsistent, does not meet known disease-resistant grade, continues that diseased plant stalk is taken to be crushed, be uniformly sprinkling upon sick nursery
It in plot, then carries out turning over earthing, continues to plant identical susceptible kind and/or middle disease-resistant variety repetitive identified, until meeting symbol
Conjunction condition can be used as root black rot Resistance Identification sick nursery.
2. according to claim 1 for identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery, feature exists
In: in the step 1), the tobacco bred of susceptible black rot, which is selected from, bar draws No. 2 and Kentucky15.
3. according to claim 1 for identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery, feature exists
In: in the step 3), in anti-black rot tobacco bred be selected from E'yan 1, HubeiT3 and Kentucky907.
4. according to claim 1 for identifying the method for building up of burley tobaccos kind root black rot resistance sick nursery, feature exists
In: in the step 4), root black rot disease incidence and sick grade of falling ill calculate disease index, the specific steps are as follows:
A. investigation 1 time before inoculation, investigation in every 5 days is primary after inoculation, investigates 5 times altogether, investigates whole cigarette strain incidences；
B. severity Scaling method:
0 grade: complete stool is disease-free；1 grade: stem's scab is no more than the one third of stem girth；3 grades: stem's scab surround three points of stem girth
One of to half；5 grades: stem's scab is more than the half of stem girth；7 grades: stem's scab is entirely around stem girth；9 grades: disease
Strain is substantially withered；
C. Evaluation standard of resistance
R (disease-resistant): 20 or less disease index；MR (in resist): disease index 20.1~40；MS (middle sense): disease index 40.1~
60；S (susceptible): disease index 60.1~80；HS (susceptible): disease index 80.1~100；
D. to investigate resulting data, the disease index of each kind is calculated as follows:
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Cited By (1)
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| CN114747445A (en) * | 2022-04-29 | 2022-07-15 | 湖北恩施中国南方马铃薯研究中心 | Method for establishing potato bacterial wilt resistance identification garden |
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Cited By (1)
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| CN114747445A (en) * | 2022-04-29 | 2022-07-15 | 湖北恩施中国南方马铃薯研究中心 | Method for establishing potato bacterial wilt resistance identification garden |
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