CN109845594A - A kind of implantation methods of multienzyme crops containing SOD and its application - Google Patents
A kind of implantation methods of multienzyme crops containing SOD and its application Download PDFInfo
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- CN109845594A CN109845594A CN201910219377.9A CN201910219377A CN109845594A CN 109845594 A CN109845594 A CN 109845594A CN 201910219377 A CN201910219377 A CN 201910219377A CN 109845594 A CN109845594 A CN 109845594A
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- multienzyme
- crops
- amino acid
- containing sod
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
Abstract
The implantation methods of the invention discloses a kind of multienzyme crops containing SOD and its application, the implantation methods include: that 1) seed of crops is dressed seed or soaked seed using amino acid fertilizer or its dilution, and the dosage of amino acid fertilizer is the corresponding amino acid fertilizer for using 5~20ml of every 10kg seed；2) foliar spray fertilising at least once is carried out in the seedling stage of crops, jointing stage, boot stage, pustulation period using amino acid fertilizer or its dilution respectively.The implantation methods make crops realize high yield and high quality, disease and insect resistance, removing because of caused by applying pesticides toxin, non-lodging, without any residual hazard, eliminate pollution of the heavy metal in soil to crops；The implantation methods can harvest the crop products rich in SOD, GSH-PX, CAT, POD and 10 kinds of microelements, directly edible to supplement above-mentioned enzyme and microelement, can be also suitably used for extracting the multienzyme liquid containing SOD.
Description
The application is the divisional application of following applications,
The applying date of original application: on May 30th, 2016,
The application number of original application: 201610371296.7,
The denomination of invention of original application: a kind of implantation methods of the multienzyme crops containing SOD, multienzyme liquid and its system containing SOD
Preparation Method.
Technical field
The invention belongs to crop planting and crop products biological processing processing technology fields, and in particular to Yi Zhonghan
The implantation methods of the multienzyme crops of SOD, also relate to the application of the implantation methods.
Background technique
Harmful free radicals are confirmed to be mankind's origin of hundreds of diseases by scientist, are the killers for threatening human health longevity.Free radical
Refer to the molecule of compound under the external conditions such as photo-thermal, homolysis occurs for covalent bond and the atom with unpaired electron that is formed
Or group.In human body, a small amount of and controlled free radical be to human body it is beneficial, can both help the energy for transmitting the vigor of sustaining life
Amount can also be used to kill bacterium and helminth, moreover it is possible to participate in excluding toxin.But when the free radical in human body is more than certain
Amount, will be out of hand, brings injury to human body.Since free radical contains unpaired electronics, so extremely unstable, therefore can be from
Capture electronics on neighbouring molecule (including fat, protein and DNA), allows and lay oneself open to stable state；So, neighbouring
Molecule become a new free radical again, then remove to capture electronics again, such chain reaction as a result,
The structure of cell is destroyed, and cell dysfunction, gene mutation, even death are caused.
Free radical to the harm major embodiment of human body in the following areas: weaken the resistance of cell, make body vulnerable to bacterium
It is infected with germ；The chemical substance for destroying cell is generated, carcinogen is formed；The normal development of block cell interferes its recovery
Function makes cell turnover rate be lower than withered rate；Intracorporal gene tissue is destroyed, running and the regeneration function of cell are upset,
Cause gene mutation；Intracellular mitochondria is destroyed, oxidation fatigue is caused；Cell membrane is destroyed, the metabolism of cell is interfered,
Cell membrane is set to lose the function of protection cell；Amino acid necessary to invasion cell tissue and hormone, interferes vivo system
Running, leads to vicious circle, so that generating more polyradical, chain reaction can lead to free radical harm throughout whole body；It destroys
Protein destroys intracorporal enzyme, leads to inflammation and aging；Fat is destroyed, makes lipid peroxidation, leads to atherosclerosis, is sent out
Raw cardiovascular and cerebrovascular disease；Carbohydrate is destroyed, makes hyaluronic acid degradation, leads to arthritis etc..Reduce the way of free radical harm
There are two types of diameters: first is that extra free radical in vivo is removed using endogenous free radical scavenger, second is that excavating exogenous antioxidant-
Free radical scavenger blocks invasion of the free radical to human body.And superoxide dismutase (SOD) is the most common radicals scavenging
Agent.
Superoxide dismutase (SOD) is a kind of active protease containing metallic element, is important in organism resist
Oxidizing ferment has special physiological activity, is the primary substance that free radical is removed in organism.SOD can be harmful super oxygen
Free radical (O2-) it is converted into hydrogen peroxide, intracorporal glutathione peroxidase (GSH-PX), catalase (CAT) and mistake
Oxide enzyme (POD) can be broken down into completely harmless water immediately；Current research confirms: SOD is removing harmful free radicals mistake
The carcinogenic free radical containing HS of one kind is had in journey to stay in the body, and more serious injury, only GHS-PX enzyme can be caused to human body
It could thoroughly be removed, four kinds of enzymes constitute a complete anti-oxidation chain.SOD is that having for ministry of Health of China approval is anti-
One of aging, immunological regulation, adjusting blood lipid, anti-radiation, cosmetology function substance；, as a kind of supplement food, people think to the greatest extent for it
Various feasible methods make SOD maintain higher level in human body, i.e., while consuming food, provide suitable nutrition supplying
Liver synthesizes SOD, or directly foreign aid SOD is wrapped in food protein, through digestive system without being digested, into body
Other positions.
In the prior art, CN105462938A discloses a kind of processing technology of Qi dragon SOD nutrition oral administration, with breeding earthworm
For raw material, clear liquid after wet-milling, homogenate, centrifugation, the clear liquid collects precipitating and uses Tris-Gly after thermal denaturation, acetone precipitation
Buffer solution takes supernatant, which chromatographs to obtain glaucous Cu, Zn SOD through thick purification, column；By gained Cu, Zn
SOD is added in the decoction medical fluid of auxiliary material Chinese medicine, obtains mixed nutrient solution and oral solution is made again.This method is extracted from pheretima
SOD is simultaneously compounded with decocting for Chinese herbal medicine liquid oral solution is made, and has the function of certain health-care assistance, but the raw material pheretima used in it is
The organism commonly cultivated, SOD content itself is low, extracts difficulty greatly and complex process, at high cost；And it is basic in gained oral solution
Containing only a kind of enzyme of SOD, the value of health-care assistance is limited, and especially this enzyme inactivates substantially after thermophilic digestion, so, SOD function
Can will not exist.
Summary of the invention
The implantation methods of the object of the present invention is to provide a kind of multienzyme crops containing SOD, SOD in gained crop products
Content is high, and POD, CAT and GHS-PX containing high level, suitable for multienzyme liquid of the extraction containing SOD.
A second object of the present invention is to provide the applications of above-mentioned implantation methods.
In order to achieve the goal above, the technical scheme adopted by the invention is that:
A kind of implantation methods of the multienzyme crops containing SOD, including the following steps:
1) seed treatment: being dressed seed or soaked seed to the seed of crops using amino acid fertilizer or its dilution, amino acid
The dosage of fertilizer is the corresponding amino acid fertilizer for using 5~20ml of every 10kg seed；
2) fertilizer treatment: using amino acid fertilizer or its dilution in the seedling stage of crops, jointing stage, boot stage, pustulation period
Foliar spray fertilising at least once is carried out respectively, and the dosage for the amino acid fertilizer that applies fertilizer every time is 50~200ml/ mus.
Wherein, the amino acid fertilizer is the amino of the amino acid solution of CN1299688C record, CN103626545A record
Any one in sour compound fertilizer or combination.
The crops are wheat, corn, rice, black rice, purple rice, red rice, oat, buckwheat, barley, naked oats, highland barley, red
Beans, pea, sorghum, millet, sesame, sunflower, potato, pachyrhizus or purple beans.Preferably, the crops are cereal crops, such as
Wheat, corn or rice.
In step 1), dilution used of dressing seed is the ratio according to the volume ratio of amino acid fertilizer and water for 1:50~1000
It is diluted；Seed soaking dilution used is dilute according to the ratio progress that the volume ratio of amino acid fertilizer and water is 1:400~1000
It releases.
In step 2), the dilution is carried out according to the ratio that the volume ratio of amino acid fertilizer and water is 1:400~2000
Dilution.
In above-mentioned implantation methods, in seedling stage, the jointing stage, boot stage, the pustulation period foliar spray fertilising can carry out respectively
More than twice, each interval time is not less than 10 days.
In the implantation methods, in addition to aforesaid operations step, remaining operates the prior art that crop planting can be used.
In above-mentioned implantation methods, crops first spread fertilizer over the fields base manure before rice transplanting or sowing and carry out site preparation again, and base manure used is
Siliceous fertilizer, dose are 50~80kg/ mus；Crops do not use chemical highly toxic pesticide and herbicide during the growth process, using life
Object pesticide and biology control worm, the technology of sterilization or product, artificial weeding.The field management of crops and watering routinely operate into
Row.
In above-mentioned implantation methods, it is to reduce grain husk flower to degenerate in the effect that boot stage carries out foliar spray fertilising, reaches guarantor's flower
Increase the purpose of grain；Carrying out foliar spray fertilising in the watery stage can make blade directly absorb fertilizer, make rapid containing nitrogen concentration in blade
Increase, improve photosynthetic capacity, this has preferable effect for accelerating grouting, raising setting percentage, increasing mass of 1000 kernel.
A kind of multienzyme liquid containing SOD is using the multienzyme crop products containing SOD of above-mentioned implantation methods harvest as raw material
Made of fermentation.A kind of preparation method of the above-mentioned multienzyme liquid containing SOD, including the following steps: (preparation method is above-mentioned
The multienzyme crops containing SOD that the implantation methods of multienzyme crops containing SOD obtain answering in multienzyme liquid of the preparation containing SOD
With)
A) the multienzyme crop products containing SOD are added in liquor potassic permanganate and are impregnated, by washing, drying, crushing, obtained
Material powder；
B) after mixing material powder obtained by step a) with water, koji for uncooked material and red yeast rice are added, in 15~55 DEG C of items
Under part, interval be sealed by fermentation 24~72h, then lasting seals ferment 24~72h, after in 12~36h of ferment under aerobic conditions, mistake
Filter to get.
In step a), multienzyme crop products used refer to the edible part of crop harvesting.Preferably, crops
Product refers to the rice of wheat whole wheat, niblet or belt leather chaff.
In step a), the mass concentration of the liquor potassic permanganate is 0.5%~4%.The time of the immersion be 5~
30min。
In step a), the crushing uses low temperature at a slow speed or air-cooled pulverizer is crushed；The temperature of crushing is not higher than
40℃。
In step b), the dosage of water are as follows: the material powder of every 200~2000g is to using water 1L.Water used is sterile
Water；Water used is in advance by sterilizing；It is described sterilizing refer in advance heat water to 100 DEG C or more 30min after, be cooled to 50
DEG C, it adds in fermentor and uses.Material powder is added in fermentor after being mixed with water, 35 DEG C of constant temperature, checks substrate without hard
Subsequent operation is carried out after the heart again.
The dosage of the koji for uncooked material are as follows: every 1L water is corresponding to be added 2.5~100g of koji for uncooked material；The dosage of the red yeast rice
Are as follows: every 1L water is corresponding to be added 20~1000g of red yeast rice.
In step b), the interval sealing and fermenting refers to that during sealing and fermenting, every 6~12h leads to atmospheric agitation 1h.It adopts
Interval sealing and fermenting is carried out with fermentor, i.e., the seal of fermentor is opened and stirs 1h by every 6~12h.
In step b), ferment under aerobic conditions after lasting seals fermentation；It ferments under aerobic conditions
It can be and open all cover boards or valve into oxygen of fermentor, oxygen is made to enter fermentor.It is carried out under aerobic conditions
It ferments after 12~36h, cooling terminates fermentation.
In step b), the filtering is filtered with the strainer of 40~80 mesh.Strainer used is food-grade strainer.Filtering
Gained clear liquid is the multienzyme liquid containing SOD afterwards.
The implantation methods of multienzyme crops containing SOD of the invention are dressed seed using amino acid fertilizer or its dilution
Or seed soaking, and using amino acid fertilizer or its dilution in seedling stage, jointing stage, boot stage, pustulation period carry out leaf at least once respectively
It applies fertilizer by spraying in face；The implantation methods can make high-quality higher yield of crops, disease and insect resistance, removing cause toxin, no because of applying pesticides
Lodge, be not afraid of natural calamity invasion, without any residual hazard, eliminate pollution of the heavy metal in soil to crops.Using this kind
Plant method plants crops, can harvest rich in superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), mistake
The farming of hydrogen oxide enzyme (CAT), peroxidase (POD) and 10 kinds of trace elements zns, Mg, Fe, Se, Sr, Mn, Mo, B, K, Cu
Produce product directly eat these crop products, can directly supplement above-mentioned enzyme and microelement；These crop products are also fitted
It shares in multienzyme liquid of the extraction containing SOD.
Particularly, the tool included using the rice that the receptor such as rice that the implantation methods harvest is harvested than traditional cultivation method
There is the enzyme material for removing free radical effect to be higher by 20% or more；It can reach after human body is edible and remove harmful free radicals in human body
Purpose；Scented rice of the mouthfeel of gained rice beyond Japan, Thailand；Traditional cultivation method of the yield of rice than using other fertilizer
High by 10% or more, mass of 1000 kernel is higher by 0.5~1g or more.
Multienzyme liquid containing SOD of the invention is that the multienzyme crop products containing SOD harvested with above-mentioned implantation methods are
Made of fermenting raw materials, it is made by fermenting raw materials of pure natural crop products, acid and alkali-resistance；It is intracorporal super to can be used for supplementing people
Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), peroxidase (POD),
Excessive free radical in human body is eliminated, human body recovery, dietotherapy, beauty food or its raw material are used as.
It is only human body supplemented with selenium element with the selenium-rich terminal receptor that selenium-rich receptor obtains, and selenium element can only be using people
The participation of the other enzymes of body, which is converted into after glutathione peroxidase, can just become real functional mass, as aging causes
Conversion capability decrease, extra selenium element can cause selenosis in people's cylinder accumulation.GHS-PX is a kind of enzyme containing selenium, can
100%, which is absorbed by the body, excessively causes heavy metal poisoning without will cause selenium element.
The preparation method of multienzyme liquid containing SOD of the invention, being will be using the crop products of above-mentioned implantation methods harvest
Through liquor potassic permanganate impregnate, rinse, air-dry after crush, after mix with water, koji for uncooked material and red yeast rice through interval sealing and fermenting,
It is filtered after lasting seals fermentation, aerobic fermentation；The preparation method simple process, it is auxiliary used in other in addition to farming raw material
Material is from a wealth of sources, at low cost；Preparation process does not need special equipment, and simple process is easy to operate, is easy to automate control
System is suitble to large-scale industrial production.
Detailed description of the invention
Fig. 1 is production system schematic diagram used in the preparation method of the multienzyme liquid containing SOD in the embodiment of the present invention 1；
Description of symbols: 1- fermentor；2- lautertuns；The filling slot of 3-；4- bottle placer；5- feed inlet；6- exhaust outlet；7-
Water inlet.
Specific embodiment
The present invention is further illustrated With reference to embodiment.
In specific embodiment, crops do not use chemical highly toxic pesticide and herbicide during the growth process, using biology
Pesticide and biology control worm, the technology of sterilization or product, artificial weeding.The field management of crops and watering routinely operate into
Row.
Embodiment 1
The implantation methods of the multienzyme rice containing SOD of the present embodiment, including the following steps:
1) seed treatment: dressing seed to rice paddy seed using the dilution of amino acid fertilizer, and the dosage of amino acid fertilizer is every
The corresponding amino acid fertilizer for using 10ml of 10kg seed, it is 1:100 that the dilution, which is according to the volume ratio of amino acid fertilizer and clear water,
Ratio be diluted；
Seedling is carried out using seedling bed after seed treatment；
2) base manure: base manure is first spread fertilizer over the fields before rice transplanting and carries out site preparation again；Base manure used is siliceous fertilizer, and dose is 50kg/ mus；
The amount of rice transplanting are as follows: early rice, ten thousand caves late rice 2-2.5/mu, ten thousand caves semilate rice 1.5-1.8/mu；
3) fertilizer treatment: distinguished using the dilution of amino acid fertilizer in the seedling stage of rice, jointing stage, boot stage, pustulation period
A foliar spray fertilising is carried out, the dosage for the amino acid fertilizer that applies fertilizer every time is 100ml/ mus, and the dilution is according to amino acid
The ratio that the volume ratio of fertilizer and clear water is 1:800 is diluted.
The amino acid fertilizer is the amino acid solution (example in the specific embodiment of CN1299688C that CN1299688C is recorded
One resulting amino acid foliage fertilizer).
The multienzyme liquid containing SOD of the present embodiment is that the multienzyme rice containing SOD of above-mentioned implantation methods harvest is sent out for raw material
Made of ferment, preparation method includes the following steps:
A) fruit (rice of belt leather chaff) the addition mass concentration of the multienzyme rice containing SOD is molten for 3% potassium permanganate
10min is impregnated in liquid, after rinsing by clear water, drying (or air-drying) processing, is put into crushing in Lowtemperaturepulverizer and (is crushed temperature not
More than 40 DEG C), obtain material powder；
B) reference water is added in fermentor, is heated to 100 DEG C or more and kept the temperature after 30min sterilized, be cooled to 50
℃；
Material powder according to every 200g material powder obtained by step a) is added in fermentor mixed with water to water 1L is applied
It closes；Under the conditions of 35 DEG C of constant temperature, after checking substrate without hard-core, koji for uncooked material 10g, red yeast rice are used according to every 1L water is corresponding
100g adds the koji for uncooked material and red yeast rice of corresponding amount；
Under the conditions of 35 DEG C of constant temperature, system interval is sealed by fermentation by 36h using fermentor, every 12h opens each of fermentor
A seal stirs 1h；Under the conditions of 35 DEG C, after fermentor is sealed by fermentation 30h, all lids into oxygen of fermentor are opened
Plate enters oxygen, at 35 DEG C of constant temperature, ferment under aerobic conditions for 24 hours, stop stirring, cooling terminate fermentation, with 60 mesh food
After grade strainer filtering, gained clear liquid is the multienzyme liquid containing SOD.
The preparation method of the multienzyme liquid containing SOD of the present embodiment, production system used are as shown in Figure 1.The production system
Including fermentor 1, lautertuns 2, filling slot 3 and the bottle placer being sequentially connected in series；The fermentor 1 be equipped with for solid material into
The feed inlet 5 of material, the water inlet 7 for water inlet and the exhaust outlet 6 for discharge gas；The fermentor 1 is that glass hair is warded off in oil bath
Fermentation tank.In the preparation process of the multienzyme liquid containing SOD, sterile water enters fermentor 1, raw material powder from water inlet 7 through water meter measurement
Body, koji for uncooked material and red yeast rice enter fermentor 1, the CO generated in fermentation process from feed inlet 52Gas is discharged from exhaust outlet 6；
Mixture after fermentation enters after lautertuns 2 is filtered, and clear liquid successively carries out through filling slot 3 and bottle placer 4 filling, obtains
50ml/ bottles of finished product.
According to detection, in multienzyme liquid of the finished product containing SOD, SOD+POD+CAT+GSH-PX >=16U/ml.
Embodiment 2
The implantation methods of the multienzyme rice containing SOD of the present embodiment, including the following steps:
1) seed treatment: soaking seed to rice paddy seed using the dilution of amino acid fertilizer, and the dosage of amino acid fertilizer is every
The corresponding amino acid fertilizer for using 5ml of 10kg seed, it is 1:600 that the dilution, which is according to the volume ratio of amino acid fertilizer and clear water,
Ratio is diluted；
Seedling is carried out using seedling bed after seed treatment；
2) base manure: base manure is first spread fertilizer over the fields before rice transplanting and carries out site preparation again；Base manure used is siliceous fertilizer, and dose is 80kg/ mus；
The amount of rice transplanting are as follows: early rice, ten thousand caves late rice 2-2.5/mu, ten thousand caves semilate rice 1.5-1.8/mu；
3) fertilizer treatment: distinguished using the dilution of amino acid fertilizer in the seedling stage of rice, jointing stage, boot stage, pustulation period
A foliar spray fertilising is carried out, the dosage for the amino acid fertilizer that applies fertilizer every time is 50ml/ mus, and the dilution is according to amino acid fertilizer
The ratio that volume ratio with clear water is 1:800 is diluted；
The amino acid fertilizer is the amino-acid compound fertilizer (specific embodiment party of CN103626545A that CN103626545A is recorded
The resulting amino-acid compound fertilizer of embodiment 1 in formula).
The multienzyme liquid containing SOD of the present embodiment is that the multienzyme rice containing SOD of above-mentioned implantation methods harvest is sent out for raw material
Made of ferment, preparation method includes the following steps:
A) fruit (rice of belt leather chaff) the addition mass concentration of the multienzyme rice containing SOD is molten for 2% potassium permanganate
20min is impregnated in liquid, after rinsing by clear water, dry processing, is put into crushing in Lowtemperaturepulverizer and (is crushed temperature and be no more than 40
DEG C), obtain material powder；
B) reference water is added in fermentor, is heated to 100 DEG C or more and kept the temperature after 30min sterilized, be cooled to 50
℃；
Material powder according to every 200g material powder obtained by step a) is added in fermentor mixed with water to water 1L is applied
It closes；Under the conditions of 35 DEG C of constant temperature, after checking substrate without hard-core, koji for uncooked material 2.5g, red yeast rice are used according to every 1L water is corresponding
20g adds the koji for uncooked material and red yeast rice of corresponding amount；
Under the conditions of 25 DEG C of constant temperature, system interval is sealed by fermentation by 48h using fermentor, every 12h opens each of fermentor
A seal stirs 1h；Under the conditions of 25 DEG C, after fermentor is sealed by fermentation 36h, all lids into oxygen of fermentor are opened
Plate enters oxygen, at 25 DEG C of constant temperature, in ferment under aerobic conditions 36h, stops stirring, cooling termination fermentation, with 40 mesh food
After grade strainer filtering, gained clear liquid is the multienzyme liquid containing SOD.
Production system used in the preparation method of the multienzyme liquid containing SOD of the present embodiment is the same as embodiment 1.
According to detection, in multienzyme liquid of the finished product containing SOD, SOD+POD+CAT+GSH-PX >=16U/ml.
Embodiment 3
The implantation methods of the multienzyme rice containing SOD of the present embodiment, including the following steps:
1) seed treatment: dressing seed to rice paddy seed using the dilution of amino acid fertilizer, and the dosage of amino acid fertilizer is every
The corresponding amino acid fertilizer for using 20ml of 10kg seed, it is 1:50 that the dilution, which is according to the volume ratio of amino acid fertilizer and clear water,
Ratio is diluted；
2) base manure: direct sowing culture is used, base manure is first spread fertilizer over the fields before planting and carries out site preparation again；Base manure used is siliceous fertilizer, is applied
Fertilizer amount is 80kg/ mus；The amount of live streaming are as follows: early rice, ten thousand seedlings of late rice 4-5/mu, ten thousand seedlings of semilate rice 3-3.5/mu；
3) fertilizer treatment: distinguished using the dilution of amino acid fertilizer in the seedling stage of rice, jointing stage, boot stage, pustulation period
A foliar spray fertilising is carried out, the dosage for the amino acid fertilizer that applies fertilizer every time is 200ml/ mus, and the dilution is according to amino acid
The ratio that the volume ratio of fertilizer and clear water is 1:600 is diluted；
Amino acid fertilizer used in step 1) is the amino acid solution (specific implementation of CN1299688C that CN1299688C is recorded
The resulting amino acid foliage fertilizer of example one in mode)；Amino acid fertilizer used in step 3) is the amino acid that CN103626545A is recorded
Compound fertilizer's (resulting amino-acid compound fertilizer of embodiment 2 in the specific embodiment of CN103626545A).
The multienzyme liquid containing SOD of the present embodiment is that the multienzyme rice containing SOD of above-mentioned implantation methods harvest is sent out for raw material
Made of ferment, preparation method includes the following steps:
A) fruit (rice of belt leather chaff) the addition mass concentration of the multienzyme rice containing SOD is molten for 4% potassium permanganate
5min is impregnated in liquid, after rinsing by clear water, dry processing, is put into crushing in Lowtemperaturepulverizer and (is crushed temperature and be no more than 40
DEG C), obtain material powder；
B) reference water is added in fermentor, is heated to 100 DEG C or more and kept the temperature after 30min sterilized, be cooled to 50
℃；
Material powder obtained by step a) is added in fermentor and water to water 1L is applied for material powder according to every 2000g
Mixing；Under the conditions of 35 DEG C of constant temperature, after checking substrate without hard-core, koji for uncooked material 100g, red yeast rice are used according to every 1L water is corresponding
1000g adds the koji for uncooked material and red yeast rice of corresponding amount；
Under the conditions of 35 DEG C of constant temperature, system interval is sealed by fermentation for 24 hours using fermentor, every 12h opens each of fermentor
A seal stirs 1h；Under the conditions of 35 DEG C, after fermentor is sealed by fermentation for 24 hours, all lids into oxygen of fermentor are opened
Plate enters oxygen, at 35 DEG C of constant temperature, in ferment under aerobic conditions 12h, stops stirring, cooling termination fermentation, with 80 mesh food
After grade strainer filtering, gained clear liquid is the multienzyme liquid containing SOD.
Production system used in the preparation method of the multienzyme liquid containing SOD of the present embodiment is the same as embodiment 1.
According to detection, in multienzyme liquid of the finished product containing SOD, SOD+POD+CAT+GSH-PX >=16U/ml.
Embodiment 4
The implantation methods of the multienzyme wheat containing SOD of the present embodiment, including the following steps:
1) seed treatment: dressing seed to wheat seed using the dilution of amino acid fertilizer, and the dosage of amino acid fertilizer is every
The corresponding amino acid fertilizer for using 15ml of 10kg seed, it is 1:200 that the dilution, which is according to the volume ratio of amino acid fertilizer and clear water,
Ratio be diluted；
2) base manure: base manure is first spread fertilizer over the fields before planting and carries out site preparation again；Base manure used is siliceous fertilizer, and dose is 80kg/ mus；
3) fertilizer treatment: distinguished using the dilution of amino acid fertilizer in the seedling stage of wheat, jointing stage, boot stage, pustulation period
A foliar spray fertilising is carried out, the dosage for the amino acid fertilizer that applies fertilizer every time is 150ml/ mus, and the dilution is according to amino acid
The ratio that the volume ratio of fertilizer and clear water is 1:800 is diluted；
Amino acid fertilizer used is the amino-acid compound fertilizer (specific implementation of CN103626545A that CN103626545A is recorded
The resulting amino-acid compound fertilizer of embodiment 3 in mode).
The multienzyme liquid containing SOD of the present embodiment is that the multienzyme wheat containing SOD of above-mentioned implantation methods harvest is sent out for raw material
Made of ferment, preparation method includes the following steps:
A) fruit (whole wheat) of the multienzyme wheat containing SOD is added in the liquor potassic permanganate that mass concentration is 3% and is impregnated
15min after rinsing by clear water, dry processing, is put into Lowtemperaturepulverizer and crushes and (crush temperature and be no more than 40 DEG C), obtain raw material
Powder；
B) reference water is added in fermentor, is heated to 100 DEG C or more and kept the temperature after 30min sterilized, be cooled to 50
℃；
Material powder according to every 500g material powder obtained by step a) is added in fermentor mixed with water to water 1L is applied
It closes；Under the conditions of 35 DEG C of constant temperature, after checking substrate without hard-core, koji for uncooked material 50g, red yeast rice are used according to every 1L water is corresponding
500g adds the koji for uncooked material and red yeast rice of corresponding amount；
Under the conditions of 30 DEG C of constant temperature, system interval is sealed by fermentation by 36h using fermentor, every 12h opens each of fermentor
A seal stirs 1h；Under the conditions of 30 DEG C, after fermentor is sealed by fermentation 30h, all lids into oxygen of fermentor are opened
Plate enters oxygen, at 30 DEG C of constant temperature, ferment under aerobic conditions for 24 hours, stop stirring, cooling terminate fermentation, with 60 mesh food
After grade strainer filtering, gained clear liquid is the multienzyme liquid containing SOD.
Production system used in the preparation method of the multienzyme liquid containing SOD of the present embodiment is the same as embodiment 1.
According to detection, in multienzyme liquid of the finished product containing SOD, SOD+POD+CAT+GSH-PX >=16U/ml.
Embodiment 5
The implantation methods of the multienzyme wheat containing SOD of the present embodiment, including the following steps:
1) seed treatment: soaking seed to wheat seed using the dilution of amino acid fertilizer, and the dosage of amino acid fertilizer is every
The corresponding amino acid fertilizer for using 15ml of 10kg seed, it is 1:1000 that the dilution, which is according to the volume ratio of amino acid fertilizer and clear water,
Ratio be diluted；
2) base manure: base manure is first spread fertilizer over the fields before planting and carries out site preparation again；Base manure used is siliceous fertilizer, and dose is 50kg/ mus；
3) fertilizer treatment: distinguished using the dilution of amino acid fertilizer in the seedling stage of wheat, jointing stage, boot stage, pustulation period
A foliar spray fertilising is carried out, the dosage for the amino acid fertilizer that applies fertilizer every time is 150ml/ mus, and the dilution is according to amino acid
The ratio that the volume ratio of fertilizer and clear water is 1:2000 is diluted；
The amino acid fertilizer is the amino acid solution (example in the specific embodiment of CN1299688C that CN1299688C is recorded
One resulting amino acid foliage fertilizer).
The multienzyme liquid containing SOD of the present embodiment is that the multienzyme wheat containing SOD of above-mentioned implantation methods harvest is sent out for raw material
Made of ferment, preparation method includes the following steps:
A) fruit (whole wheat) of the multienzyme wheat containing SOD is added in the liquor potassic permanganate that mass concentration is 0.5% and is soaked
30min is steeped, after rinsing by clear water, dry processing, is put into Lowtemperaturepulverizer and crushes and (crush temperature and be no more than 40 DEG C), obtain former
Feed powder body；
B) reference water is added in fermentor, is heated to 100 DEG C or more and kept the temperature after 30min sterilized, be cooled to 50
℃；
Material powder according to every 500g material powder obtained by step a) is added in fermentor mixed with water to water 1L is applied
It closes；Under the conditions of 55 DEG C of constant temperature, after checking substrate without hard-core, koji for uncooked material 50g, red yeast rice are used according to every 1L water is corresponding
500g adds the koji for uncooked material and red yeast rice of corresponding amount；
Under the conditions of 30 DEG C of constant temperature, system interval is sealed by fermentation by 36h using fermentor, every 12h opens each of fermentor
A seal stirs 1h；Under the conditions of 30 DEG C, after fermentor is sealed by fermentation 30h, all lids into oxygen of fermentor are opened
Plate enters oxygen, at 30 DEG C of constant temperature, ferment under aerobic conditions for 24 hours, stop stirring, cooling terminate fermentation, with 60 mesh food
After grade strainer filtering, gained clear liquid is the multienzyme liquid containing SOD.
Production system used in the preparation method of the multienzyme liquid containing SOD of the present embodiment is the same as embodiment 1.
According to detection, in multienzyme liquid of the finished product containing SOD, SOD+POD+CAT+GSH-PX >=16U/ml.
Embodiment 6
The implantation methods of the multienzyme corn containing SOD of the present embodiment, including the following steps:
1) seed treatment: dressing seed to corn seed using the dilution of amino acid fertilizer, and the dosage of amino acid fertilizer is every
The corresponding amino acid fertilizer for using 15ml of 10kg seed, it is 1:50 that the dilution, which is according to the volume ratio of amino acid fertilizer and clear water,
Ratio is diluted；
2) base manure: base manure is first spread fertilizer over the fields before planting and carries out site preparation again；Base manure used is siliceous fertilizer, and dose is 60kg/ mus；
3) fertilizer treatment: distinguished using the dilution of amino acid fertilizer in the seedling stage of corn, jointing stage, boot stage, pustulation period
A foliar spray fertilising is carried out, the dosage for the amino acid fertilizer that applies fertilizer every time is 100ml/ mus, and the dilution is according to amino acid
The ratio that the volume ratio of fertilizer and clear water is 1:800 is diluted.
Amino acid fertilizer used is the amino-acid compound fertilizer (specific implementation of CN103626545A that CN103626545A is recorded
The resulting amino-acid compound fertilizer of embodiment 3 in mode).
The multienzyme liquid containing SOD of the present embodiment is that the multienzyme corn containing SOD of above-mentioned implantation methods harvest is sent out for raw material
Made of ferment, preparation method includes the following steps:
A) fruit of the multienzyme corn containing SOD is added in the liquor potassic permanganate that mass concentration is 3% and impregnates 10min,
After rinsing by clear water, dry processing, it is put into Lowtemperaturepulverizer and crushes and (crush temperature and be no more than 40 DEG C), obtain material powder；
B) reference water is added in fermentor, is heated to 100 DEG C or more and kept the temperature after 30min sterilized, be cooled to 50
℃；
Material powder obtained by step a) is added in fermentor and water to water 1L is applied for material powder according to every 1500g
Mixing；Under the conditions of 35 DEG C of constant temperature, after checking substrate without hard-core, koji for uncooked material 50g, red yeast rice are used according to every 1L water is corresponding
500g adds the koji for uncooked material and red yeast rice of corresponding amount；
Under the conditions of 25 DEG C of constant temperature, system interval is sealed by fermentation by 36h using fermentor, every 12h opens each of fermentor
A seal stirs 1h；Under the conditions of 25 DEG C, after fermentor is sealed by fermentation 30h, all lids into oxygen of fermentor are opened
Plate enters oxygen, at 25 DEG C of constant temperature, ferment under aerobic conditions for 24 hours, stop stirring, cooling terminate fermentation, with 60 mesh food
After grade strainer filtering, gained clear liquid is the multienzyme liquid containing SOD.
Production system used in the preparation method of the multienzyme liquid containing SOD of the present embodiment is the same as embodiment 1.
According to detection, in multienzyme liquid of the finished product containing SOD, SOD+POD+CAT+GSH-PX >=16U/ml.
In other embodiments of the invention, the crops planted can also be the crops of other wheats or rice class, such as
Black rice, purple rice, red rice, oat, buckwheat, barley, naked oats, highland barley, sorghum or millet, are sowed by the way of live streaming, are broadcast
Kind amount determines that remaining is the same as embodiment 1 by existing conventional techniques.
In other embodiments of the invention, the crops planted can also be other crops, such as beans such as red bean, pea
Beans or purple beans or potato such as potato or pachyrhizus, are sowed by the way of live streaming, and application rate is determined by existing conventional techniques,
Remaining is the same as embodiment 1.
Experimental example 1
Activity and micronutrient levels of this experimental example to the antioxidase of the multienzyme rice containing SOD obtained by embodiment 1-3
It is detected；Testing result is as shown in table 1,2.
Activities of antioxidant enzymes detection method are as follows: nitroblue tetrazolium method, guaiacol method, ultraviolet absorption method etc..Testing result is such as
Shown in table 1.Comparative example 1-3 is the implantation methods for not applying amino acid fertilizer in table 1, remaining is respectively the same as embodiment 1-3.
The testing result of multienzyme rice containing SOD obtained by 1 embodiment 1-3 of table (test object is full rice)
The detection foundation of microelement is GB-T 5009.91-2003, GB 5009.93-2010, DB53/T 288-
2009.Key instrument used: atomic fluorescence spectrophotometry is detected, etc..
The Results of Trace Element Detection of multienzyme rice containing SOD obtained by 2 embodiment 1-3 of table (test object is rice)
Testing result shows: the tool included using the rice that the rice that the method for the present invention harvests is harvested than traditional cultivation method
There is the enzyme material for removing free radical effect to be higher by 20% or more, and contains various trace elements.
Simultaneously according to statistics, compared with comparative example 1-3, the implantation methods of the multienzyme rice containing SOD of embodiment 1-3, rice
Setting percentage averagely improve 5.7%~5.8%, mass of 1000 kernel increases 0.5g, and output per hectare improves 324~426kg.
Experimental example 2
This experimental example detects the activity of the antioxidase of the multienzyme wheat containing SOD of embodiment 4,5.It is arranged simultaneously
Comparative example 4,5, comparative example 4,5 are not apply the implantation methods of amino acid fertilizer, remaining is respectively the same as embodiment 4,5.
Detection method are as follows: spectrophotometry, ultraviolet absorption method, NBT method etc..Testing result is as shown in table 3.
The testing result of 3 embodiment of table, 4,5 multienzyme wheat of the gained containing SOD (test object is wholemeal)
The detection foundation of microelement is GB-T 5009.91-2003, GB 5009.93-2010, DB53/T 288-
2009.Key instrument used: atomic fluorescence spectrophotometry is detected, etc..
The Results of Trace Element Detection of 4 embodiment of table, 4,5 multienzyme wheat of the gained containing SOD (test object is wheat)
Testing result shows: the tool included using the wheat that the wheat that the method for the present invention harvests is harvested than traditional cultivation method
There is the enzyme material for removing free radical effect to be higher by one times or more, and contains various trace elements.
Experimental example 3
This experimental example detects the activities of antioxidant enzymes of the multienzyme liquid containing SOD obtained by embodiment 1-6.Detection method is
Nitroblue tetrazolium method, guaiacol method, ultraviolet absorption method etc..The results are shown in Table 5.
The activities of antioxidant enzymes testing result of multienzyme liquid containing SOD obtained by 5 embodiment 1-6 of table
Claims (10)
1. a kind of implantation methods of the multienzyme crops containing SOD, it is characterised in that: include the following steps:
1) seed treatment: being dressed seed or soaked seed to the seed of crops using amino acid fertilizer or its dilution, amino acid fertilizer
Dosage is the corresponding amino acid fertilizer for using 5~20ml of every 10kg seed；
2) fertilizer treatment: distinguished using amino acid fertilizer or its dilution in the seedling stage of crops, jointing stage, boot stage, pustulation period
It carries out foliar spray at least once to apply fertilizer, the dosage for the amino acid fertilizer that applies fertilizer every time is 50~200ml/ mus.
2. the implantation methods of the multienzyme crops according to claim 1 containing SOD, it is characterised in that: the crops are
Wheat, corn, rice, black rice, purple rice, red rice, oat, buckwheat, barley, naked oats, highland barley, red bean, pea, sorghum, millet, sesame
Fiber crops, sunflower, potato, pachyrhizus or purple beans.
3. the implantation methods of the multienzyme crops according to claim 1 containing SOD, it is characterised in that: in step 1), seed dressing
Dilution used is diluted according to the ratio that the volume ratio of amino acid fertilizer and water is 1:50~1000；It is dilute used in seed soaking
Releasing liquid is diluted according to the ratio that the volume ratio of amino acid fertilizer and water is 1:400~1000.
4. the implantation methods of the multienzyme crops according to claim 1 containing SOD, it is characterised in that: described in step 2
Dilution is diluted according to the ratio that the volume ratio of amino acid fertilizer and water is 1:400~2000.
5. the implantation methods of the multienzyme crops according to claim 1 containing SOD, it is characterised in that: in seedling stage, jointing
The foliar spray fertilising of phase, boot stage, pustulation period can carry out respectively more than twice, and each interval time is not less than 10 days.
6. the implantation methods of the multienzyme crops according to claim 1 containing SOD, it is characterised in that: crops are in rice transplanting
Or first spread fertilizer over the fields base manure before sowing and carry out site preparation again, base manure used is siliceous fertilizer, and dose is 50~80kg/ mus.
7. the multienzyme crops containing SOD obtained using the implantation methods of the multienzyme crops containing SOD as described in claim 1
Application in multienzyme liquid of the preparation containing SOD.
8. the multienzyme crops containing SOD that the implantation methods of the multienzyme crops according to claim 7 containing SOD obtain exist
Prepare the application in the multienzyme liquid containing SOD, it is characterised in that: include the following steps:
A) the multienzyme crop products containing SOD are added in liquor potassic permanganate and are impregnated, by washing, drying, crushing, obtain raw material
Powder；
B) after mixing material powder obtained by step a) with water, koji for uncooked material and red yeast rice are added, under the conditions of 15~55 DEG C,
Interval is sealed by fermentation 24~72h, then lasting seals ferment 24~72h, after in 12~36h of ferment under aerobic conditions, filtering, i.e.,
?.
9. the multienzyme crops containing SOD that the implantation methods of the multienzyme crops according to claim 8 containing SOD obtain exist
Prepare the application in the multienzyme liquid containing SOD, it is characterised in that: the dosage of the koji for uncooked material are as follows: every 1L water is corresponding to be added raw material
2.5~100g of distiller's yeast；The dosage of the red yeast rice are as follows: every 1L water is corresponding to be added 20~1000g of red yeast rice.
10. the multienzyme crops containing SOD that the implantation methods of the multienzyme crops according to claim 7 containing SOD obtain
Application in multienzyme liquid of the preparation containing SOD, it is characterised in that: in step b), the interval sealing and fermenting is to be sealed by fermentation
In the process, every 6~12h leads to atmospheric agitation 1h.
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| CN106818873A (en) * | 2016-12-27 | 2017-06-13 | 唐林元 | A kind of preparation method for improving wheat frost resistance spacetabs type seed soak |
| CN106879947A (en) * | 2017-04-07 | 2017-06-23 | 伍宝年 | A kind of deciduous teeth tire rice zymotechnique |
| CN107541502A (en) * | 2017-04-12 | 2018-01-05 | 路传章 | The method that SOD multienzyme alkalescence oral liquids are prepared with the cereal crops rich in SOD multienzyme |
| CN107467448A (en) * | 2017-04-12 | 2017-12-15 | 路传章 | A kind of cereal beverage containing superoxide dismutase and preparation method thereof |
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| CN107373256A (en) * | 2017-04-12 | 2017-11-24 | 路传章 | A kind of multienzyme fruit drink containing SOD and preparation method thereof |
| CN107593334A (en) * | 2017-08-28 | 2018-01-19 | 常熟新晟现代农业科技有限公司 | A kind of implantation methods of high SOD contents rice |
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| CN109220647B (en) * | 2018-01-30 | 2021-07-13 | 五月阳光生物科技(浙江)有限公司 | Planting method of rice with high trace elements and high enzyme activity |
| CN108718836A (en) * | 2018-04-20 | 2018-11-02 | 申琳 | A kind of method of the unknown disease of prevention and control green plants |
| CN108718956A (en) * | 2018-04-20 | 2018-11-02 | 申琳 | A method of adjusting wheat plant shape |
| CN109121532A (en) * | 2018-07-13 | 2019-01-04 | 高俪萍 | A kind of SOD function implantation methods |
| CN109089728B (en) * | 2018-08-09 | 2021-12-07 | 合肥福泉现代农业科技有限公司 | Method for efficiently cultivating edible fungi by enzymolysis and fermentation of soybean and highland barley |
| CN109548596B (en) * | 2018-12-27 | 2021-10-08 | 长沙谷奥生物科技有限公司 | Method for cultivating high SOD active corn |
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