CN107541502A - The method that SOD multienzyme alkalescence oral liquids are prepared with the cereal crops rich in SOD multienzyme - Google Patents

Abstract

The present invention relates to the method that SOD multienzyme alkalescence oral liquids are prepared with the cereal crops rich in SOD multienzyme.This method includes：1) pulvis is made after will be enriched in the cereal crops crushing of SOD multienzyme, pulvis is mixed with water, obtains mixed liquor A；2) amylase and activator are added into mixed liquor A, 25~35min is digested at 60 DEG C~85 DEG C, obtains mixed liquid B；The pH value for adjusting mixed liquid B is 8~12, adds alkali cellulose enzyme and protease, and 4h~5h is digested at 40~50 DEG C, obtains mixed liquor C；Beer complex enzyme, distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out under conditions of 30~40 DEG C, obtains zymotic fluid；Alkaline pectase is added into zymotic fluid, the stirring reaction at 35~50 DEG C, filtering, is produced.This method maintains the primitive environment of the multienzyme such as SOD in cereal crops, and the activity of each function factor is high, and healthcare function is good.

Description

The method that SOD multienzyme alkalescence oral liquids are prepared with the cereal crops rich in SOD multienzyme

Technical field

The invention belongs to nutrition oral administration field, and in particular to prepare SOD multienzyme alkali with the cereal crops rich in SOD multienzyme The method of property oral liquid.

Background technology

The presence of harmful free radicals can be destroyed the structure of cell in human body, cause cell dysfunction, gene to dash forward Become even dead.Superoxide dismutase (SOD) is a kind of active protease containing metallic element, is important in organism Antioxidase, there is special physiologically active, be the primary material that superoxide radical is removed in organism.SOD can be harmful Superoxide radical be converted into hydrogen peroxide, internal glutathione peroxidase (GSH-PX), catalase (CAT) and Peroxidase (POD) is broken down into completely harmless water again.SOD has a kind of carcinogenic during harmful free radicals are removed The free radical containing HS stay in the body, more serious injury can be caused to human body, tetra- kinds of enzymes compositions of SOD, POD, CAT and GSH-PX Anti-oxidation chain can effectively eliminate excessive free radicals in human body, maintain the normal development of cell.

Supplemented with exogenous free love base scavenger is one of important channel for reducing free radical harm.Application publication number is CN105462938A patent discloses a kind of processing technology of Qi dragon SOD nutrition oral administrations, and it is using breeding earthworm as raw material, warp Clear liquid is taken after wet-milling, homogenate, centrifugation, after clear liquid thermal denaturation, acetone precipitation, precipitation is collected and uses Tris-Gly buffer solutions, Supernatant is taken, the supernatant obtains glaucous Cu, Zn SOD through thick purification, column chromatography；Gained Cu, Zn SOD are added into auxiliary material In the decoction decoction of Chinese medicine, obtain mixed nutrient solution and oral liquid is made again.In oral liquid obtained by this method, SOD enzymes are through high temperature Inactivated substantially after boiling, its own function can not be played.

The content of the invention

It is an object of the invention to provide a kind of SOD multienzyme alkalescence oral liquids are prepared with the cereal crops rich in SOD multienzyme Method, so as to solve the problems, such as that SOD's in the existing oral liquid containing SOD is active low.

To achieve the above object, the technical scheme of the multienzyme oral liquid of the invention containing SOD is：

The method that SOD multienzyme alkalescence oral liquids are prepared with the cereal crops rich in SOD multienzyme, comprises the following steps：

1) pulvis is made after will be enriched in the cereal crops crushing of SOD multienzyme, pulvis is mixed with water, obtains mixed liquor A；

2) amylase and activator are added into mixed liquor A, 25~35min is digested at 60 DEG C~85 DEG C, is mixed Liquid B；

The pH value for adjusting mixed liquid B is 8~12, adds alkali cellulose enzyme and protease, 4h is digested at 40~50 DEG C ~5h, obtain mixed liquor C；

Beer complex enzyme, distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out under conditions of 30~40 DEG C, is sent out Zymotic fluid；

Alkaline pectase is added into zymotic fluid, the stirring reaction at 35~50 DEG C, filtering, is produced.

The method that cereal crops of the use of the present invention rich in SOD multienzyme prepare SOD multienzyme alkalescence oral liquids, with rich in SOD The cereal crops of multienzyme are raw material, by enzyme digestion reaction and microbial fermentation, make the starch in cereal crops and protein conversion Decompose, promote the abundant release of the multienzyme such as SOD；This method maintains the primitive environment of the multienzyme such as SOD, the activity of each function factor The functional components such as height, not easy in inactivation after long-term storage, the SOD in oral liquid are easily absorbed by the human body, and healthcare function is good.

In step 1), the cereal crops rich in SOD multienzyme are oat, barley, wheat, corn, one kind in rice or more Kind combination.Preferably, the cereal crops rich in SOD multienzyme are that cultivation obtains by the following method, and this method includes：Sowing Phase, seed is soaked seed or dressed seed using amino acid fertilizer；In tri-leaf period, jointing stage, boot stage, pustulation period, to cereal crops Root soil, stem, leaf site sprinkling amino acid fertilizer.

The amino acid fertilizer is that the amino acid that Freamine Ⅲ, the CN103626545B that CN1299688C is recorded are recorded is compound Any one in fertilizer or combination.The above method cultivates obtained crop acceptor, rich in SOD, GSH-PX, CAT and POD, also contains There are 10 kinds of trace elements such as Zn, Mg, Fe, Se, Sr, Mn, Mo, B, K, Cu；Using above-mentioned cereal crops as raw material, oral liquid can be made Functional component species is more abundant, nutrient balance, health properties are more preferable.

In step 1), the mass ratio of pulvis and water is (20~40)：100.

In step 2), the amylase is mesophilicα-diastase, and the activator is calcium chloride；Medium temperature is corresponded to per kg pulvis The addition of alpha-amylase is 1~3ml, and the addition of corresponding calcium chloride is 0.01~0.03g；The enzyme of the mesophilicα-diastase Vigor is 4000~8000u/ml.

In step 2), the addition that alkali cellulose enzyme is corresponded to per kg pulvis is 2~3g, and the addition of corresponding protease is 4~5g；The enzyme activity of alkali cellulose enzyme is 100,000~300,000 u/g, and the enzyme activity of protease is 50,000~200,000 u/g.

In step 2), the addition that beer complex enzyme is corresponded to per kg pulvis is 0.5~1ml, and the addition of corresponding distiller's yeast is 0.8~1.4g；The beer complex enzyme contains the enzyme activity of 1,4 beta-glucanase, zytase and protease, wherein 1,4 beta-glucanase Power >=8300u/ml, enzyme activity >=2800u/ml of zytase, enzyme activity >=30000u/ml of protease.

In step 2), the time of the anaerobic fermentation is 6~8 days；Preferably, the 1st~3 day of anaerobic fermentation, per 10h~ 14h carries out air agitation, and the 4th day to fermentation ends, air agitation is carried out per 24h.Stirring to zymotic fluid surface flotation has one layer Untill grain pulvis.During fermentation ends, no suspended substance in zymotic fluid can smell strong aroma, and grain pulvis hand pinches no hard-core.

In step 2), the addition that alkaline pectase is corresponded to per kg pulvis is 0.5g~1.0g.The alkaline pectase is excellent Elect acid alkaline pectase as；The time of stirring reaction is 1~3h.

The cereal crops method for preparing SOD multienzyme alkalescence oral liquid of the use of the present invention rich in SOD multienzyme, from rich in SOD, GSH-PX, CAT and POD cereal crops are raw material, are carried out by above-mentioned preferably enzyme digestion reaction and microorganism fermentation process The activity of the multienzyme such as processing, SOD is high, and various functions composition is easily absorbed by the human body, and can effectively remove super oxygen excessive in human body certainly By base, health care, cosmetology function are good.This method technique is simple, auxiliary material wide material sources, and cost is low；Preparation process need not be special Equipment, technique is simple, easy to operate, is easy to Automated condtrol, be adapted to large-scale industrial production.

Embodiment

Embodiments of the present invention are described further with reference to specific embodiment.In following examples, with oat Exemplified by, the cultivation of the cereal crops rich in SOD multienzyme is carried out, the amino acid fertilizer used is molten for the amino acid that CN1299688C is recorded Liquid, in sowing time, dressed seed using 400~800 times of dilutions of Freamine Ⅲ, tri-leaf period, the jointing stage, boot stage, It is pustulation period, sprayable in the watery stage using 800 times of dilutions of Freamine Ⅲ to oat root soil, stem, leaf even spraying More than twice, each interval time is not less than 10 days；In addition to aforesaid operations step, remaining operation can use the existing of Oatplanting Technology.

Crops first spread fertilizer over the fields base manure and carry out site preparation again before planting, and base manure uses bio-fertilizer, and chemical fertilizer is using survey soil Formulation stage fertilizer practice, worm, the technology of sterilization or product, artificial weeding are controlled using biological pesticide and biology.The field of crops Between management and watering routinely operation carry out.

The above-mentioned cereal crops rich in multienzyme such as SOD can directly be processed into multiple product, such as multienzyme pulvis health protection tea, multienzyme Feed and barley pulvis tea serial health product.Multienzyme pulvis health protection tea can use following processing method：(1) cereal crops are sent Enter in sterile workshop, ozone sterilization 8h, then reuse the liquor potassic permanganate that mass concentration is 0.4% and soak 5~10min, Further sterilization removes residual hazard；Then cleaned using clean water, centrifuge is entered after cleaning and dries surface moisture, decortication, is done Dry material；(2) dried material is delivered between stone mill workshop or the nanometer pulverization of desinfection chamber, by ten grades of grindings or nano-equipment Afterwards, finished product is made into vacuum packing machine, commodity packing machine, through examining, the E. CoIi content of the health protection tea meets edible state Family's standard, SOD+POD+CAT+GSH-PX >=30u/mg.

Multienzyme root, stem and leaf bar after harvesting barley wheat is directly crushed into packing, you can as multienzyme feed, multienzyme feed Middle SOD+POD+CAT+GSH-PX >=20u/mg.

Above-mentioned cultural method can also be used and make grain seedling multienzyme green leaf tea, be specially：(1) selection is in Greenhouse Water Culture ware Or contaminated soil is planted, 400~800 times of dilutions immersion seed 30min of preceding amino acid fertilizer is sowed, then will kind Son dries sowing；(2) 800 times of dilutions of rich water selection amino acid foliage fertilizer；During soil cultivation, seedling stage used amino every 10 days 800 times of dilutions of sour foliar fertilizer are to seedling root soil and blade face even spraying；(3) water planting and soil drought are planted, and height of seedling 200mm is opened Begin to gather in, after the wheat seeding cut off is protected into blue or green dry below 60 DEG C, crush, packaging, produce multienzyme green leaf tea.Through examining, this is more SOD+POD+CAT+GSH-PX >=30u/mg in enzyme green leaf tea.

Beer complex enzyme, mesophilicα-diastase belong to commercially available conventional products, and it is limited that summer Sheng biotechnology exploitation such as may be selected The Related product of company, wherein beer complex enzyme contain 1,4 beta-glucanase, zytase and protease, wherein 1,4 beta-glucanase Enzyme activity >=8300u/ml, enzyme activity >=2800u/ml of zytase, enzyme activity >=30000u/ml of protease；It is alkaline fine The enzyme activity for tieing up plain enzyme is 200,000 u/g, and the enzyme activity of protease is 150,000 u/g.Distiller's yeast used is to make every effort to overcome distiller's yeast.

Embodiment 1

The method that cereal crops of the use of the present embodiment rich in SOD multienzyme prepare SOD multienzyme alkalescence oral liquids, use are following Step：

1) in sterile workshop, oat whole grain is cleaned with sterilized water after physical sterilization 20min, got rid of (without decortication) It is dry, breaker is sent into by sealed conveying device, crushed as the pulvis of the mesh of 20 mesh~100；150kg powder is added into fermentation tank Agent, the mixing of 500kg water is added, obtains mixed liquor A；

2) mesophilicα-diastase 150ml (enzyme activity 6000u/ml), calcium chloride 3.0g, 75 are added into mixed liquor A 30min is digested at DEG C, obtains mixed liquid B；

3) pH value that mixed liquid B is adjusted with sodium bicarbonate is 8~12, adds alkali cellulose enzyme 450g, protease 750g, 4h is digested at 45 DEG C, obtains mixed liquor C；

4) by mixed liquor C greenhouse cooling to 30 DEG C, beer complex enzyme 150ml, distiller's yeast 133g are added, is sent out in 30 DEG C of anaerobism Ferment 7 days, wherein the 1st day to the 3rd day, per 12h, opening stirring once, from the 4th day, stirring is open per 24h once, mixing control liquid Untill face floating has one layer of grain pulvis, zymotic fluid is obtained；

Alkaline pectase 100g (enzyme activity is 200,000 u/g) is added into zymotic fluid, in 35 DEG C of stirring reaction 2h, stands 2h After filter, produce oral liquid finished product.

Embodiment 2

The method that cereal crops of the use of the present embodiment rich in SOD multienzyme prepare SOD multienzyme alkalescence oral liquids, use are following Step：

1) in sterile workshop, barley whole grain is cleaned with sterilized water after physical sterilization 20min, got rid of (without decortication) It is dry, breaker is sent into by sealed conveying device, crushed as the pulvis of the mesh of 20 mesh~100；150kg powder is added into fermentation tank Agent, the mixing of 500kg water is added, obtains mixed liquor A；

2) mesophilicα-diastase 180ml (enzyme activity 6000u/ml), calcium chloride 3g, at 60 DEG C are added into mixed liquor A Lower enzymolysis 30min, obtains mixed liquid B；

3) pH value that mixed liquid B is adjusted with sodium bicarbonate is 8~12, adds alkali cellulose enzyme 400g, protease 700g, 5h is digested at 40 DEG C, obtains mixed liquor C；

4) by mixed liquor C greenhouse cooling to 35 DEG C, beer complex enzyme 159ml, distiller's yeast 199g are added, is sent out in 35 DEG C of anaerobism Ferment 7 days, wherein the 1st day to the 3rd day, per 12h, opening stirring once, from the 4th day, stirring is open per 24h once, mixing control liquid Untill face floating has one layer of grain pulvis, zymotic fluid is obtained；

Alkaline pectase 150g (enzyme activity is 200,000 u/g) is added into zymotic fluid, in 45 DEG C of stirring reaction 2h, stands 2h After filter, produce oral liquid finished product.

Embodiment 3

The method that cereal crops of the use of the present embodiment rich in SOD multienzyme prepare SOD multienzyme alkalescence oral liquids, use are following Step：

1) in sterile workshop, wheat whole grain is cleaned with sterilized water after physical sterilization 20min, got rid of (without decortication) It is dry, breaker is sent into by sealed conveying device, crushed as the pulvis of the mesh of 20 mesh~100；150kg powder is added into fermentation tank Agent, the mixing of 500kg water is added, obtains mixed liquor A；

2) mesophilicα-diastase 150ml (enzyme activity 6000u/ml), calcium chloride 3g, at 80 DEG C are added into mixed liquor A Lower enzymolysis 30min, obtains mixed liquid B；

3) pH value that mixed liquid B is adjusted with sodium bicarbonate is 12, adds alkali cellulose enzyme 450g, protease 750g, 50 5h is digested at DEG C, obtains mixed liquor C；

4) by mixed liquor C greenhouse cooling to 35 DEG C, beer complex enzyme 159ml, distiller's yeast 199g are added, is sent out in 35 DEG C of anaerobism Ferment 7 days, wherein the 1st day to the 3rd day, per 12h, opening stirring once, from the 4th day, stirring is open per 24h once, mixing control liquid Untill face floating has one layer of grain pulvis, zymotic fluid is obtained；

Alkaline pectase 120g (enzyme activity is 200,000 u/g) is added into zymotic fluid, in 45 DEG C of stirring reaction 2h, stands 2h After filter, produce oral liquid finished product.

In other embodiments of the invention, the cereal crops such as the corn rich in SOD multienzyme, rice may be selected is raw material, According to the technological parameter of embodiment 1, the suitable SOD multienzyme alkalescence oral liquids of functional component are prepared.

Test example

This test example detects the functional enzyme content of the gained multienzyme alkalescence oral liquid of 1~embodiment of embodiment 3 and stored Enzymatic activity after regular period keeps situation (condition of storage is room temperature), as a result as shown in Table 1 to Table 3.

The content and active holding situation of the functional enzyme of the multienzyme alkalescence oral liquid of the embodiment 1 of table 1

The content and active holding situation of the functional enzyme of the multienzyme alkalescence oral liquid of the embodiment 2 of table 2

The content and active holding situation of the functional enzyme of the multienzyme alkalescence oral liquid of the embodiment 3 of table 3

Above-mentioned preparation method provided by the invention, on the one hand using rich in SOD enzymes, CAT enzymes, POD enzymes, the grain of GSH-PX enzymes Food crop is raw material, and above-mentioned functional enzyme is entered in human body by " cereal crops → fermentation → human body " approach, is had higher Security and absorbability, on the other hand, this method remains endogenous SOD enzymes, CAT enzymes, POD enzymes, the GSH- of cereal crops The primitive environment of PX enzymes, the interfering material that above-mentioned functional enzyme can be promoted to convert or combine at utmost is eliminated, so as to have It is stable beneficial to the long period of activity of above-mentioned functional enzyme.

The storage temperature condition of existing SOD oral liquids requirement is generally 4 DEG C~8 DEG C, and storage can be with maximum journey at low temperature Degree avoids SOD enzymes from inactivating.And the gained multienzyme alkalescence oral liquid of 1~embodiment of embodiment 3 is after normal temperature storage eight months, enzymatic activity Keep all right, reflect better enzymatic structure stability.What is more important, exogenous sod oral liquid is because only focusing on The supplement of single SOD enzymes, SOD can produce a kind of carcinogenic free radical containing HS during free radical is removed and stay in the body, instead And more serious injury can be caused to human body.In the multienzyme alkalescence oral liquid of the present invention, the activity of the enzyme such as CAT, POD, GSH-PX It is maintained, these enzymes constitute the working chain for removing above-mentioned harmful free radicals, and SOD enzymes are together with coenzyme such as CAT, POD, GSH-PX The complete chain for removing harmful free radicals is together constituted, so as to be more beneficial for keeping body health.

Claims (8)

1. the method for preparing SOD multienzyme alkalescence oral liquids with the cereal crops rich in SOD multienzyme, it is characterised in that including following Step：

1) pulvis is made after will be enriched in the cereal crops crushing of SOD multienzyme, pulvis is mixed with water, obtains mixed liquor A；

2) amylase and activator are added into mixed liquor A, 25~35min is digested at 60 DEG C~85 DEG C, obtains mixed liquid B；

The pH value for adjusting mixed liquid B is 8~12, adds alkali cellulose enzyme and protease, and 4h~5h is digested at 40~50 DEG C, Obtain mixed liquor C；

Beer complex enzyme, distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out under conditions of 30~40 DEG C, obtains zymotic fluid；

Alkaline pectase is added into zymotic fluid, the stirring reaction at 35~50 DEG C, filtering, is produced.

2. the method as described in claim 1, it is characterised in that the cereal crops rich in SOD multienzyme are to plant by the following method Training obtains, and this method includes：In sowing time, seed is soaked seed or dressed seed using amino acid fertilizer；Tri-leaf period, the jointing stage, Boot stage, pustulation period, spray root soil from amino acid fertilizer to cereal crops, stem, leaf site.

3. the method as described in claim 1, it is characterised in that the mass ratio of pulvis and water is (20~40)：100.

4. the method as described in claim 1, it is characterised in that in step 2), the amylase is mesophilicα-diastase, described Activator is calcium chloride；The addition that mesophilicα-diastase is corresponded to per kg pulvis is 1~3ml, and the addition of corresponding calcium chloride is 0.01~0.03g；The enzyme activity of the mesophilicα-diastase is 4000~8000u/ml.

5. the method as described in claim 1, it is characterised in that in step 2), the addition of beer complex enzyme is corresponded to per kg pulvis Measure as 0.5~1ml, the addition of corresponding distiller's yeast is 0.8~1.4g；The beer complex enzyme contains 1,4 beta-glucanase, xylan Enzyme activity >=8300u/ml of enzyme and protease, wherein 1,4 beta-glucanase, enzyme activity >=2800u/ml of zytase, protease Enzyme activity >=30000u/ml.

6. the method as described in claim 1, it is characterised in that in step 2), the time of the anaerobic fermentation is 6~8 days.

7. method as claimed in claim 6, it is characterised in that the 1st~3 day of anaerobic fermentation, ventilated per 10h~14h Stirring, the 4th day to fermentation ends, air agitation is carried out per 24h.

8. the method as described in claim 1, it is characterised in that in step 2), the addition of alkaline pectase is corresponded to per kg pulvis Measure as 0.5g~1.0g.