CN108238828A - A kind of agricultural biomass source soilless culture Selenium-rich nutrient solution and preparation method thereof - Google Patents
A kind of agricultural biomass source soilless culture Selenium-rich nutrient solution and preparation method thereof Download PDFInfo
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- CN108238828A CN108238828A CN201810174742.4A CN201810174742A CN108238828A CN 108238828 A CN108238828 A CN 108238828A CN 201810174742 A CN201810174742 A CN 201810174742A CN 108238828 A CN108238828 A CN 108238828A
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- enzymolysis
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
- C05C5/02—Fertilisers containing other nitrates containing sodium or potassium nitrate
Abstract
The present invention relates to a kind of agricultural biomass source soilless culture Selenium-rich nutrient solutions and preparation method thereof.The nutrient solution using biomass straw waste material be raw material as the source of selenium element, pass through and crush, enzymolysis, centrifugation, obtains biomass straw waste disposal liquid, then with mineral salt, amino acid, urea, folic acid, composite growth regulator, oxidized form of glutathione, deionized water are mixed in a certain ratio, mixed solution is stirred using magnetic stirring apparatus, then through centrifugation, takes supernatant, finally dialysed with nanometer film, the Selenium-rich nutrient solution after being filtered.Soilless culture Selenium-rich nutrient solution in agricultural biomass source produced by the present invention can not only discharge the natural plant active factor, promote crop root system growth, the immunity that crops resist pest and disease damage can be improved simultaneously, promoted crops photosynthesis, improved crop yield and quality.
Description
Technical field
The present invention relates to a kind of nutrient solution and preparation method thereof, more particularly to a kind of agricultural biomass source soilless culture richness
Selenium nutrient solution and preparation method thereof.
Background technology
Tank farming is also known as nutrition cultivation, water culture.It is not have to soil in planting plants, not stercoration, one
As nutrient needed for the nutrient solution supply crop that is only made into chemical fertilizer or chemical reagent special cultural method.Soilless culture phase
Than traditional soil cultivation, by the less-restrictive of edaphic condition, while productive labor intensity is low, largely saved into
This.The liquid manure supply of nutrient fluid cultivation generally using microchannel irrigation system, is easier to carry out fixed point, localization, can obviously reduce
The waste of liquid manure.Experiment proves that, than water-saving 3-7 times of soil cultivation, utilization rate of fertilizer improves 20%~30%.
In recent years, the hot spot of cultivation technique without soil is also become for the research of soilless culture nutrient fluid, in the prior art
Based on inorganic nutrient solution, most basic nutritional need is mainly provided for crops for the soilless culture nutrient fluid largely used.
Itself the problem is that, these soilless culture nutrient fluids only provide most basic nutritional need for crops, but crops
The hypoimmunity of itself, the ability for resisting pest and disease damage is weaker, and the survival rate and yield for causing crops reduce.
The present invention provides a kind of biomass sources Selenium-rich nutrient solutions suitable for soilless culture, pass through biomass waste material richness
Collect selenium element, through highly purified, the techniques such as compounding are made, while a kind of composite growth regulator is added in nutrient solution, pass through
Mutual synergistic effect significantly improves absorption of the crops to nutrient, promotes photosynthesis, improves crops and resists
The ability of insect pest improves crop yield, while realizes recycling for biological waste.
Invention content
It is relatively low to the applicability of crops to solve inorganic soilless culture nutrient fluid in the prior art, while nutritional ingredient
Absorptivity is relatively low, causes crops survival rate low and yield declines, the crop and fruit grown is in the side such as size, color, mouthfeel
The problems such as face is undesirable.The present invention provides a kind of agricultural biomass source soilless culture Selenium-rich nutrient solution, the nutrient solution be from
Organic Selenium is extracted in biomass waste material, is made through techniques such as highly concentrated, compoundings, the natural plant active factor can not only be discharged,
Promote crop root system growth, while the immunity that crops resist pest and disease damage can be improved, promote crops photosynthesis, improve
Crop yield and quality.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of agricultural biomass source soilless culture Selenium-rich nutrient solution, the biomass sources soilless culture Selenium-rich nutrient solution is by following heavy
The Raw material processing of amount part forms：150-250 parts of biomass waste material treatment fluid, 15-25 parts of mineral salt, 5-25 parts of amino acid 1, urine
It is 15-25 parts plain, 5-10 parts of composite growth regulator, 5-10 parts of folic acid, 5-10 parts of oxidized form of glutathione.
The preparation method of the biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）It by Se-enriched bio matter waste material, is crushed with pulverizer, crosses 80-100 mesh sieve；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, milling time 10-30min, power 10-30HZ are obtained
Raw material is digested, carries out preservation at room temperature；
3）By step 2）In enzymolysis raw material and water 1:3-5（Weight）Mixing, and stir evenly, pH value is adjusted to a 5.6-6.7 left sides
The right side adds in the cellulase of waste material quality 0.8%-1.2% and is stirred continuously, enzymolysis time 2-4h, 45-55 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 30-50 DEG C in 110-120 DEG C, heating 3-5s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 4000-6000rmp, centrifugation time 5-
10min obtains enzymolysis clear liquid；
6）By step 5）Enzymolysis clear liquid with NF membrane carry out dialysis concentration 1-3 times, the Se-enriched bio matter waste material after being concentrated
Treatment fluid；
7）6 will be walked）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth tune
Section agent and oxidized form of glutathione are uniformly mixed by formula ratio, are filtered off water-insoluble impurity, are obtained Selenium-rich nutrient solution finished product.
Preferably, the Se-enriched bio matter waste material is stalk, selenium-rich bacteria stick, husk waste material.
Preferably, the mineral salt for potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate by weight（0.8-1.2）:
（0.8-1.2）:（0.5-1.2）:（0.5-1.2）Composition.
Preferably, the preparation method step of the oxidized form of glutathione is as follows：
1）Reduced glutathione is taken, in 40-45 DEG C of dissolving, the reduced glutathione solution of 50-100g/L is obtained, surveys its pH
It is worth for A1；
2) with barium hydroxide tune pH to A2,7.0≤A2≤7.2；
3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.2-0.5h after adding；
4）After reaction, a certain amount of manganese dioxide is added in, stirs 5-10min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3, A1-0.1≤A3≤A1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
It is furthermore preferred that the dosage of the hydrogen peroxide is 0.4-0.5ml/g glutathione, the mass fraction of hydrogen peroxide is 20-
30%。
Preferably, the composite growth regulator is formed by the Raw material processing of following parts by weight：Digest seaweed residue dry powder
15-25 parts, 5-10 parts of avenabeta glucosan, 0.5-1 parts of polylysine, 1-2 parts of heteroauxin.
It is furthermore preferred that the preparation method of composite growth regulator is as follows：
1）Dry seaweed residue is added in a certain amount of inorganic salt solution, impregnates 30min；
2）It is beaten at a high speed, PH to 6.5-6.8 is adjusted after mashing, add in the wetting and penetrating agent and 2%- of dry seaweed mass of residue 2%-3%
3% complex enzyme zymohydrolysis 30-50min, 45-55 DEG C of hydrolysis temperature, after be heated to 80-90 DEG C of enzyme deactivation 3-5s, be cooled to 30-50
℃；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, centrifuge speed 4000-6000rmp, centrifugation time 5-
10min takes supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 150-500Da,
Enzymolysis seaweed extracted liquor after being filtered；
5）Enzymolysis seaweed extracted liquor after filtering is further concentrated under 60-80 DEG C of temperature, vacuum degree -0.075-0.095mpa
It is 30-40% to solid content, is then dried to obtain the powdered enzymolysis seaweed that moisture content is 4%-7% in spray dryer again
Residue dry powder；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 80-100 mesh sieve, the growth regulator after being compounded.
Preferably, the wetting and penetrating agent is neopelex, lauryl sodium sulfate, alkylphenol-polyethenoxy
The one or more of mixing of ether, sorbitan fatty acid ester -20, the composition of complex enzyme is one kind in cellulase and protease
Or two kinds of mixing.
The beneficial effects of the invention are as follows：The present invention by using biomass waste material be enriched with selenium element, concentrated purification, separately
Other natural nutritions and inorganic substances are added in, the nutrient solution product is obtained through compounding, the absorption of crop nutrient elements can be promoted,
Sufficient nutrition for the plant growth full stage is provided, while improves the ability that crops resist pest and disease damage, promotes plant photosynthesis
Effect improves crop yield and quality.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1：
The preparation method of biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）It by stalk waste material, is crushed with pulverizer, crosses 80 mesh sieve；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, it is former to obtain enzymolysis by milling time 10min, power 10HZ
Material, carries out preservation at room temperature；
3）By step 2）In enzymolysis raw material and water 1:3（Weight）Mixing, and stir evenly, pH value is adjusted to 5.6 or so, is added in
The cellulase of waste material quality 0.8% is simultaneously stirred continuously, enzymolysis time 2h, 45 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 30 DEG C in 110 DEG C, heating 3s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 4000rmp, centrifugation time 5min are obtained
Digest clear liquid；
6）By step 5）Enzymolysis clear liquid carry out 1 times of dialysis concentration with NF membrane, at the Se-enriched bio matter waste material after being concentrated
Manage liquid；
7）6 will be walked）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth tune
Section agent and oxidized form of glutathione are uniformly mixed by formula ratio, are filtered off water-insoluble impurity, are obtained Selenium-rich nutrient solution finished product.
The mineral salt is potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate are by weight 0.8:0.8:0.5:0.5 composition.
The preparation method step of the oxidized form of glutathione is as follows：
1）Reduced glutathione is taken, in 40 DEG C of dissolvings, the reduced glutathione solution of 50g/L is obtained, surveys its pH value as A1；
2) with barium hydroxide tune pH to A2=7.0；
3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.2h after adding；
4）After reaction, a certain amount of manganese dioxide is added in, stirs 5min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3=A1-0.1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
The mass fraction of the hydrogen peroxide is 20%, and dosage is 0.4ml/g glutathione.
The composite growth regulator is formed by the Raw material processing of following parts by weight：Digest 15 parts of seaweed residue dry powder, swallow
5 parts of wheat beta glucan, 0.5 part of polylysine, 1 part of heteroauxin.
The preparation method of composite growth regulator is as follows：
1）30g dry seaweed residues are added in water and inorganic salt solution, impregnate 30min；
2）It is beaten at a high speed, PH to 6.5 is adjusted after mashing, add in 2% wetting and penetrating agent and 2% complex enzyme zymohydrolysis 30min, enzymolysis temperature
Degree 45 DEG C, after be heated to 80 DEG C of enzyme deactivation 3s, be cooled to 30 DEG C；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, and centrifuge speed 4000rmp, centrifugation time 5min take
Supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 150Da, is obtained
Enzymolysis seaweed extracted liquor after filtering；
5）Enzymolysis seaweed extracted liquor after filtering is further concentrated into solid content under temperature 60 C, vacuum degree -0.075mpa
Content is 30%, is then dried to obtain the powdered enzymolysis seaweed residue dry powder that moisture content is 4% in spray dryer again；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 80 mesh sieve, the growth regulator after being compounded.
Embodiment two：
The preparation method of biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）It by selenium-rich bacteria stick waste material, is crushed with pulverizer, crosses 90 mesh sieve；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, it is former to obtain enzymolysis by milling time 20min, power 20HZ
Material, carries out preservation at room temperature；
3）By step 2）In enzymolysis raw material and water 1:4（Weight）Mixing, and stir evenly, pH value is adjusted to 6 or so, is added in useless
Expect the cellulase of quality 1% and be stirred continuously, enzymolysis time 3h, 55 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 40 DEG C in 115 DEG C, heating 4s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 5000rmp, centrifugation time 7.5min,
Clear liquid must be digested；
6）By step 5）Enzymolysis clear liquid carry out 2 times of dialysis concentration with NF membrane, at the Se-enriched bio matter waste material after being concentrated
Manage liquid；
7）By step 6）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth
Conditioning agent and oxidized form of glutathione are uniformly mixed by formula ratio, are filtered off water-insoluble impurity, are obtained Selenium-rich nutrient solution finished product.
The mineral salt is potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate are by weight 1:1:0.8:0.8 composition.
The preparation method step of the oxidized form of glutathione is as follows：
1）Reduced glutathione is taken, in 42 DEG C of dissolvings, the reduced glutathione solution of 75g/L is obtained, surveys its pH value as A1；
2) with barium hydroxide tune pH to A2=7.1；
3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.35h after adding；
4）After reaction, a certain amount of manganese dioxide is added in, stirs 7.5min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3=A1-0.1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
The mass fraction of the hydrogen peroxide is 25%, and dosage is 0.45ml/g glutathione.
The composite growth regulator is formed by the Raw material processing of following parts by weight：Digest 20 parts of seaweed residue dry powder, swallow
8 parts of wheat beta glucan, 0.75 part of polylysine, 1.5 parts of heteroauxin.
The preparation method of composite growth regulator is as follows：
1）Dry seaweed residue is added in water and inorganic salt solution, impregnates 30min；
2）It is beaten at a high speed, PH to 6 is adjusted after mashing, add in 2.5% wetting and penetrating agent and 2.5% complex enzyme zymohydrolysis 40min, enzymolysis
Temperature 50 C, after be heated to 85 DEG C of enzyme deactivation 3.5s, be cooled to 40 DEG C；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, and centrifuge speed 5000rmp, centrifugation time 8min take
Supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 300Da, is obtained
Enzymolysis seaweed extracted liquor after filtering；
5）The enzymolysis seaweed extracted liquor after filtering is further concentrated into solid content under temperature 70 C, vacuum degree 0.015mpa to contain
It is 35% to measure, and is then dried to obtain the powdered enzymolysis seaweed residue dry powder that moisture content is 6% in spray dryer again；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 90 mesh sieve, the growth regulator after being compounded.
Embodiment 3：
The preparation method of biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）It by husk waste material, is crushed, sieved with 100 mesh sieve with pulverizer；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, it is former to obtain enzymolysis by milling time 30min, power 30HZ
Material, carries out preservation at room temperature；
3）By step（2）In enzymolysis raw material and water 1:5（Weight）Mixing, and stir evenly, pH value is adjusted to 6.7 or so, is added
Enter the cellulase of waste material quality 1.2% and be stirred continuously, enzymolysis time 4h, 55 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 50 DEG C in 120 DEG C, heating 5s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 6000rmp, centrifugation time 10min are obtained
Digest clear liquid；
6）By step 5）Enzymolysis clear liquid carry out 3 times of dialysis concentration with NF membrane, at the Se-enriched bio matter waste material after being concentrated
Manage liquid；
7）6 will be walked）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth tune
Section agent and oxidized form of glutathione are uniformly mixed by formula ratio, are filtered off water-insoluble impurity, are obtained Selenium-rich nutrient solution finished product.
The mineral salt is potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate are by weight 1.2:1.2:1.2:1.2 composition.
The preparation method step of the oxidized form of glutathione is as follows：
1）Reduced glutathione is taken, in 45 DEG C of dissolvings, obtains the reduced glutathione solution of 100g/L, surveying its pH value is
A1；
2) with barium hydroxide tune pH to A2=7.2；
3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.5h after adding；
4）After reaction, a certain amount of manganese dioxide is added in, stirs 10min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3=A1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
The mass fraction of the hydrogen peroxide is 30%, and dosage is 0.5ml/g glutathione.
The composite growth regulator is formed by the Raw material processing of following parts by weight：Digest 25 parts of seaweed residue dry powder, swallow
10 parts of wheat beta glucan, 1 part of polylysine, 2 parts of heteroauxin.
The preparation method of composite growth regulator is as follows：
1）Dry seaweed residue is added in water and inorganic salt solution, impregnates 30min；
2）It is beaten at a high speed, PH to 6.8 is adjusted after mashing, adds in 3% alkyl phenol polyoxyethylene ether and 3% amylase and protease
Mixture digest 50min, 55 DEG C of hydrolysis temperature, after be heated to 90 DEG C of enzyme deactivation 5s, be cooled to 50 DEG C；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, and centrifuge speed 6000rmp, centrifugation time 10min take
Supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 500Da, is obtained
Enzymolysis seaweed extracted liquor after filtering；
5）The enzymolysis seaweed extracted liquor after filtering is further concentrated into solid content under 80 DEG C of temperature, vacuum degree 0.095mpa to contain
It is 40% to measure, and is then dried to obtain the powdered enzymolysis seaweed residue dry powder that moisture content is 7% in spray dryer again；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 100 mesh sieve, the growth regulator after being compounded.
The present invention provides a kind of biomass sources Selenium-rich nutrient solutions suitable for soilless culture, pass through biomass waste material richness
Collect selenium element, through highly purified, the techniques such as compounding are made, while a kind of composite growth regulator is added in nutrient solution, pass through
Mutual synergistic effect significantly improves absorption of the crops to nutrient, promotes photosynthesis, improves crops and resists
The ability of insect pest improves crop yield, while realizes recycling for biological waste.
Embodiment described above is a kind of preferable scheme of the present invention, and not the present invention is made in any form
Limitation also has other variants and remodeling under the premise of without departing from the technical solution recorded in claim.
Claims (9)
- A kind of 1. agricultural biomass source soilless culture Selenium-rich nutrient solution, which is characterized in that the biomass sources soilless culture selenium-rich Nutrient solution is formed by the Raw material processing of following parts by weight：150-250 parts of Se-enriched bio matter waste disposal liquid, mineral salt 15-25 Part, 5-25 parts of amino acid 1,15-25 parts of urea, 5-10 parts of composite growth regulator, 5-10 parts of folic acid, oxidized form of glutathione 5- 10 parts.
- 2. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as described in claim 1, feature exist In including the following steps：S1, by Se-enriched bio matter waste material, crushed with pulverizer, cross 80-100 mesh sieve；S2, superfine grinding is carried out to the waste material after crushing using ball milling instrument, milling time 10-30min, power 10-30HZ are obtained Raw material is digested, carries out preservation at room temperature；S3, by the enzymolysis raw material in step S2 and water in mass ratio 1:3-5 is mixed, and is stirred evenly, and adjusts pH value to 5.6- 6.7, it adds in the cellulase of waste material quality 0.8%-1.2% and is stirred continuously, enzymolysis time 2-4h, 45-55 DEG C of hydrolysis temperature；After S4, enzymolysis, enzymolysis liquid heats 3-5s enzyme deactivations, is cooled to 30-50 DEG C in 110-120 DEG C；S5, the enzymolysis liquid after step S4 inactivations is centrifuged using centrifuge, centrifuge speed 4000-6000rmp, centrifugation time 5- 10min obtains enzymolysis clear liquid；S6, the enzymolysis clear liquid of step S5 is subjected to 1-3 times of dialysis concentration, the Se-enriched bio matter waste material after being concentrated with NF membrane Treatment fluid；S7, by the obtained Se-enriched bio matter waste disposal liquid of step S6 and mineral salt, amino acid, urea, folic acid, composite growth Conditioning agent and oxidized form of glutathione are uniformly mixed by formula ratio, are filtered off water-insoluble impurity, are obtained Selenium-rich nutrient solution finished product.
- 3. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as described in claim 1, feature exist In：The Se-enriched bio matter waste material is selenium-rich stalk, selenium-rich bacteria stick and selenium-rich husk waste material.
- 4. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as described in claim 1, feature exist In, the mineral salt be potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate by weight（0.8-1.2）:（0.8-1.2）:（0.5- 1.2）:（0.5-1.2）Composition.
- 5. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as described in claim 1, feature exist In the preparation method step of the oxidized form of glutathione is as follows：S1, reduced glutathione is taken, in 40-45 DEG C of dissolving, obtains the reduced glutathione solution of 50-100g/L, survey it PH value is A1；S2, with barium hydroxide tune pH to A2,7.0≤A2≤7.2；S3, a certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.2-0.5h after adding；S4, after reaction adds in a certain amount of manganese dioxide, stirs 5-10min, then fully filters off manganese dioxide；S5, with sulphur acid for adjusting pH to A3, A1-0.1≤A3≤A1；S6, barium sulfate precipitate is fully filtered off, be spray-dried, obtain oxidized form of glutathione.
- 6. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as claimed in claim 5, feature exist In the mass fraction of the hydrogen peroxide is 20-30%, and dosage is 0.4-0.5ml/g glutathione.
- 7. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as described in claim 1, feature exist In the composite growth regulator is formed by the Raw material processing of following parts by weight：Digest 15-25 parts of seaweed residue dry powder, oat 5-10 parts of beta glucan, 0.5-1 parts of polylysine, 1-2 parts of heteroauxin.
- 8. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as claimed in claim 7, feature exist In the composite growth regulator preparation method is as follows：S1, dry seaweed residue is added in a certain amount of inorganic salt solution, impregnates 30-40min；S2, be beaten at a high speed, adjust pH to 6.5-6.8 after mashing, the wetting and penetrating agent of addition dry seaweed mass of residue 2%-3% and The complex enzyme zymohydrolysis 30-50min of 2%-3%, 45-55 DEG C of hydrolysis temperature, after be heated to 80-90 DEG C of enzyme deactivation 3-5s, be cooled to 30-50℃；S3, step S2 enzymolysis solution after enzyme deactivation is centrifuged using centrifuge, centrifuge speed 4000-6000rmp, centrifugation time 5- 10min takes supernatant, to digest seaweed extracted liquor；S4, enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 150- 500Da, the enzymolysis seaweed extracted liquor after being filtered；S5, by the enzymolysis seaweed extracted liquor after filtering under 60-80 DEG C of temperature, vacuum degree -0.075-0.095mpa further it is dense Solid content is reduced to as 30-40%, is then dried to obtain the powdered enzymolysis sea that moisture content is 4%-7% in spray dryer again Algae residue dry powder；S6, by the obtained enzymolysis seaweed residue dry powder of step S5 and avenabeta glucosan, polylysine, heteroauxin presses formula ratio It is uniformly mixed, is sieved with 80-100 mesh sieve, obtains composite growth regulator.
- 9. a kind of preparation method of agricultural biomass source soilless culture Selenium-rich nutrient solution as claimed in claim 8, feature exist In the wetting and penetrating agent is neopelex, lauryl sodium sulfate, alkyl phenol polyoxyethylene ether, Sorbitan The one or more of mixing of alcohol fatty acid ester -20, the composition of complex enzyme is arbitrary two in cellulase, amylase and protease Kind or three kinds of mixing.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113105289A (en) * | 2021-04-13 | 2021-07-13 | 安徽珍硒农业科技有限责任公司 | Selenium-rich organic nutrient solution for fruit crop cultivation and preparation method thereof |
| CN113860963A (en) * | 2021-09-14 | 2021-12-31 | 湖北国硒科技发展有限公司 | Organic selenium-rich nutrient solution and preparation method and application thereof |
| CN114249619A (en) * | 2022-01-20 | 2022-03-29 | 黑龙江省昊千生物科技有限公司 | Foliar application fertilizer for rapidly increasing selenium content of crops |
| WO2023171749A1 (en) * | 2022-03-09 | 2023-09-14 | 株式会社カネカ | Composition for covering plant seed |
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| CN102190525A (en) * | 2010-03-18 | 2011-09-21 | 李林奇 | Selenium-rich plant nutrient solution and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113105289A (en) * | 2021-04-13 | 2021-07-13 | 安徽珍硒农业科技有限责任公司 | Selenium-rich organic nutrient solution for fruit crop cultivation and preparation method thereof |
| CN113860963A (en) * | 2021-09-14 | 2021-12-31 | 湖北国硒科技发展有限公司 | Organic selenium-rich nutrient solution and preparation method and application thereof |
| CN114249619A (en) * | 2022-01-20 | 2022-03-29 | 黑龙江省昊千生物科技有限公司 | Foliar application fertilizer for rapidly increasing selenium content of crops |
| CN114249619B (en) * | 2022-01-20 | 2023-06-16 | 黑龙江省昊千生物科技有限公司 | Foliar fertilizer capable of rapidly improving selenium content of crops |
| WO2023171749A1 (en) * | 2022-03-09 | 2023-09-14 | 株式会社カネカ | Composition for covering plant seed |
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