CN108848975B - Method for improving cassava disease resistance through betaine treatment - Google Patents
Method for improving cassava disease resistance through betaine treatment Download PDFInfo
- Publication number
- CN108848975B CN108848975B CN201810602395.0A CN201810602395A CN108848975B CN 108848975 B CN108848975 B CN 108848975B CN 201810602395 A CN201810602395 A CN 201810602395A CN 108848975 B CN108848975 B CN 108848975B
- Authority
- CN
- China
- Prior art keywords
- cassava
- betaine
- improving
- disease resistance
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 title claims abstract description 98
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 title claims abstract description 81
- 229960003237 betaine Drugs 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 29
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 title claims abstract 8
- 208000035240 Disease Resistance Diseases 0.000 title abstract description 24
- 238000011282 treatment Methods 0.000 title description 5
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 title 1
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 82
- 230000001580 bacterial effect Effects 0.000 claims abstract description 17
- 238000011419 induction treatment Methods 0.000 claims abstract description 15
- 238000005507 spraying Methods 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 20
- 239000002689 soil Substances 0.000 claims description 9
- 210000003608 fece Anatomy 0.000 claims description 6
- 239000010871 livestock manure Substances 0.000 claims description 6
- 241000589634 Xanthomonas Species 0.000 claims description 4
- 239000003337 fertilizer Substances 0.000 claims description 4
- 230000004720 fertilization Effects 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 21
- 201000010099 disease Diseases 0.000 description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 239000000463 material Substances 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000036579 abiotic stress Effects 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000221785 Erysiphales Species 0.000 description 2
- 244000017020 Ipomoea batatas Species 0.000 description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 208000017520 skin disease Diseases 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000609458 Corynespora Species 0.000 description 1
- 241000371644 Curvularia ravenelii Species 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- 235000004456 Manihot esculenta Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000008723 osmotic stress Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 125000001453 quaternary ammonium group Chemical group 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for improving cassava disease resistance through betaine induction treatment, which comprises the following steps: continuously spraying exogenous substance betaine solution with the concentration of 25-400 mu M to the leaf surface of the cassava seedlings in any period of the growth stage for 5-14 days. The method is easy to implement, simple and convenient to operate, and quick in effect, can improve the disease resistance of the cassava to the bacterial wilt in a short time, and has great significance for maintaining the continuous and healthy development of the cassava industry.
Description
Technical Field
The invention belongs to the technical field of cassava disease resistance, and particularly relates to a method for improving the cassava disease resistance through short-time betaine treatment.
Background
Cassava (Manihot esculenta Crantz), belonging to the genus Manihot of the family Euphorbiaceae, is one of three potato crops (potato, sweet potato and cassava) in the world, has the reputation of underground granaries, starch king and special crops, is a tropical subtropical high-starch crop, is also a main food source of more than seven hundred million people in the world, and has wide application in the aspects of food, feed processing, industrial raw materials and the like. The cassava has the advantages of drought and barren resistance, easy growth, low requirement on land, relatively low production cost, high photosynthetic efficiency and the like, and is widely planted in the world. In recent years, the cassava planting area in China reaches more than 50 million hectares, and is second to rice, sweet potatoes, sugarcane and corn. The plants are planted in provinces, autonomous regions and direct prefectures such as Guangdong, Guangxi, Hainan, Jiangxi and Yunnan, Fujian, Sichuan, Chongqing, Guizhou, Hunan, Zhejiang and Taiwan, and are mainly concentrated in provinces such as Guangxi, Guangdong, Yunnan, Hainan and Jiangxi. According to statistics, the planting area and the yield of the Guangxi Zhuang autonomous region only account for more than 60 percent of the whole country.
Because the cassava is suitable for planting areas and has superior conditions such as light, heat, moisture and the like and rich biological diversity, the cassava is very beneficial to the generation and harm of various diseases, pests and weeds. Foreign cassava diseases are reported to include bacterial wilt, mosaic virus disease, brown spot, anthracnose, helminthosporium leaf spot, corynespora leaf spot, white spot, phytophthora root rot, powdery mildew, brown streak, frog skin disease and the like. The main diseases include mosaic virus disease, brown streak disease, powdery mildew and frog skin disease. At present, the diseases harmful to domestic cassava mainly comprise bacterial wilt, root rot and brown spot of cassava.
The cassava bacterial wilt disease is a bacterial disease caused by Xanthomonas axacuminata (cassava wilt pathogenic variety pv. manihotis) and is one of the internationally important quarantine diseases. With the continuous development of the cassava industry in China, germplasm and products are frequently exchanged with other countries, and worldwide diseases such as bacterial wilt and the like occur in main cassava planting areas in China and are very serious in the situation. In production, the disease is mainly prevented and controlled by methods such as seedling quarantine, biological prevention and control, medicament prevention and control and the like, and an effective prevention and control method is not available. Therefore, exogenous treatment is carried out on cassava to improve the disease resistance of the cassava in a short time so as to reduce the influence of diseases on the cassava industry and stably improve the cassava industry, and the method becomes a realistic problem to be solved for the continuous and healthy development of the cassava industry.
Betaine is a quaternary ammonium type water-soluble alkaloid, is very easy to dissolve in water, methanol, ethanol and ether, can generate trimethylamine through decomposition reaction of concentrated potassium hydroxide solution, has hygroscopicity and is very easy to deliquesce, and can release the trimethylamine. Betaine is used as an osmotic regulator and an enzyme protective agent, and accumulation of betaine enables a plurality of metabolic key enzymes to continuously keep activity under osmotic stress, and the integrity of cell membranes under stress is maintained to a certain extent. Researches on abiotic stress show that betaine has a certain effect on abiotic stress such as drought and salt, but the researches on the abiotic stress of plants are rarely reported.
How to further improve the disease resistance of cassava and enable the cassava to be well planted under the condition of relevant environmental stress is a hot spot of current research. At present, no method for rapidly improving the cassava disease resistance exists, so that the method has great significance for improving the cassava disease resistance by utilizing exogenous substances to treat the cassava so as to maintain the continuous and healthy development of the cassava industry.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a method for improving the disease resistance of plants, in particular to a method for improving the disease resistance of cassava through betaine induction treatment. The method is easy to implement, simple and convenient to operate, takes effect quickly, can improve the disease resistance of the cassava in a short time, and has great significance for maintaining the sustainable and healthy development of the cassava industry.
In order to achieve the above object, the present inventors have creatively induced cassava seedlings with a foreign substance, betaine, to obtain a method for improving the disease resistance of cassava in a short time. Specifically, the invention adopts the following technical scheme:
a method for improving the disease resistance of cassava through betaine induction treatment, wherein the method comprises the following steps: continuously spraying betaine solution with the concentration of 25-400 mu M to the leaf surface of the cassava seedlings in any period of the growth stage for 5-14 days.
Further preferably, the method for improving the cassava disease resistance by betaine induction treatment as described above, wherein the height of cassava seedlings when the betaine solution is sprayed is 15-30 cm.
Further preferably, the method for improving the disease resistance of cassava by betaine induction treatment as described above, wherein the concentration of the betaine solution is 50 μ M to 200 μ M.
Further preferably, the method for improving the cassava disease resistance through betaine induction treatment is as described above, wherein the time for continuously spraying the betaine solution is 7-9 days and 2 times/day.
Still further preferably, the method for improving the disease resistance of cassava through betaine induction treatment further comprises the steps of fertilizing, deep ploughing, leveling and planting the seedling growing land before seedling growing, and watering from 1 st to 40 th days after seedling growing; wherein:
the fertilizing step is that enough base fertilizers are applied at one time, including decomposed farmyard manure 2000-;
the planting step adopts a stem cuttage method, the cuttage density is 5 per square meter from early spring to late spring, 2/3 with the length of seed stems and the ground form an angle of 15-45 degrees and are buried in planting ditches, 1/3 of the seed stems expose surface soil, after cuttage, the seeds are pressed to enable the stems to contact with the soil, and the stems are watered thoroughly to keep the soil moist;
watering for 500-; 300-40 d watering, 600L/mu/day, and irrigating by rainwater under natural conditions after 40 days.
Further preferably, the method for improving the disease resistance of cassava through betaine induction treatment is as described above, wherein the cassava is deeply turned for 15-20 cm after fertilization, and then a seedling raising land is leveled.
Tests of the present invention show that by continuously spraying different low-concentration betaine solutions in the range of 50 μ M to 200 μ M (molar mass concentration, i.e. 5.857-23.43mg betaine per litre water) for a short period of time (7 days) the treated cassava material was found to be more resistant to diseases than the untreated cassava material. As shown in the attached drawing, when the cassava is not treated, the bacteria surviving on the cassava multiply faster, the bacteria number is more, when the cassava is infected for 6 days, the log value of the bacteria number reaches about 6.9, and the bacteria number of the cassava can reach about 5.3 after the cassava is treated by low-concentration betaine solution of 50-200 mu M (molar mass concentration, namely 5.857-23.43mg of betaine is contained in each liter of water); that is, the bacteria count of the cassava materials treated with the low-concentration betaine solution of 50 μ M to 200 μ M (molar mass concentration, i.e. containing 5.857 to 23.43mg betaine per liter of water) is about 3/4 of the bacteria count of the cassava materials not treated. Based on the research results, the invention also provides the application of betaine in the aspect of cassava resistance physiological induction technology, namely: the betaine is applied to improving the disease resistance of cassava. Further preferably, wherein said disease is bacterial wilt.
Compared with the prior art, the method for improving the cassava disease resistance through betaine induction treatment has the following advantages and progresses: the method is easy to implement, simple and convenient to operate, and quick in effect, can improve the disease resistance of the cassava to the bacterial wilt in a short time, and has great significance for maintaining the continuous and healthy development of the cassava industry.
Drawings
FIG. 1 is a schematic diagram showing the effect of continuous spraying of betaine solutions of different concentrations on the resistance of cassava to bacterial blight bacteria of cassava after 7 days of induction treatment.
The cassava material growing normally for 20 days is divided into four parts on average, the first part is sprayed with clear water for 7 days, the second part is sprayed with 50 mu M betaine (with molar mass concentration, namely, each liter of water contains 5.857mg of betaine) solution for 7 days, the third part is sprayed with 100 mu M betaine (with molar mass concentration, namely, each liter of water contains 11.715mg of betaine) solution for 7 days, and the fourth part is sprayed with 200 mu M betaine (with molar mass concentration, namely, each liter of water contains 23.43mg of betaine) solution for 7 days. Four material injections were inoculated Xam and samples were taken for bacterial counts.
Detailed Description
The following is a specific example related to the present invention, and further description is made on the technical scheme of the present invention, but the protection scope of the present invention is not limited to the example. All changes, modifications and equivalents that do not depart from the spirit of the invention are intended to be included within the scope thereof.
Example 1: test example for improving cassava disease resistance by betaine Induction treatment
A. Planting plants:
fertilizing the seedling raising field, deeply turning for 15-20 cm, and leveling. Applying enough base fertilizer once every 667m23000kg of decomposed farmyard manure (including pig manure, cow manure and sheep manure) is applied to 2000-one area, 40-60kg of compound fertilizer (containing high-concentration potassium sulfate and nitrate nitrogen, the total nutrient is not less than 45%) is added to each mu, and 50-60kg of ammonium bicarbonate is applied to each mu. Leveling the bed surface and preparing the seedbed.
Cassava is planted by adopting a stem cuttage method. The planting is carried out in late spring (4 months bottom), and the planting density is 5 per square meter. Burying 2/3 with seed stem length in planting ditch at 15/20/25/30/35/40/45 degree with ground, exposing 1/3 of seed stem to surface soil, cutting, and pressing to make stem contact with soil. Watering thoroughly to keep soil moist. After the seed stem is cut at 15/20/25/30/35/40/45 degrees with the ground, the seed stem can germinate and sprout about a week.
B. Seedling stage management:
watering every 667m at the early stage of seedling culture2Watering for 500-1000L, and keeping the ground dry; 20 days before seedling, each 667m2Watering for 500-1000L; 20-40 days, each 667m2Watering for 300-600L.
C. And (3) betaine induction treatment:
selecting cassava seedlings (10-15 cm higher) with the size of 20d, continuously spraying different low-concentration betaine solutions (the molar mass concentration is that each liter of water contains 5.857-23.43mg of betaine) to the leaf surfaces for 50-200 mu M (twice a day) in 7 days; watering according to normal conditions or irrigating by rainwater according to natural conditions in other times; 20-40 days, each 667m2Watering for 300-; after 40 days, irrigating with rainwater according to natural conditions.
D. Inoculation Xam and statistics of bacterial count:
activating cassava bacterial wilt pathogen (xanthomonas carpi) stored in the laboratory, namely a wilting pathogenic variety of the xanthomonas carpi, which is separated from cassava leaves in teaching bases of the institute of agriculture and forestry, tropics, university of Hainan, on an LB (10 g of NaCl, 10g of tryptone and 5g of yeast extract per liter) plate, picking the single bacterium, dropping the single bacterium into 10mL of LB culture solution for culturing (28 ℃, 180rpm) for 12 hours, transferring the bacterium solution to a new LB culture solution for continuous culture until the concentration of the bacterium solution reaches OD (OD)6000.6. After centrifugation at 4000rpm for 10min at room temperature, the supernatant was discarded. With 10mM MgCl2Solution (molar mass concentration, i.e. containing 95.21mg MgCl per litre water)2) Suspend and dilute the bacterial solution to OD600About 0.2, injecting the bacterial liquid to mesophyll on both sides of the main vein by using an injector. At different time points, taking the injection point as the center of a circle, taking leaf discs with consistent sizes by using a puncher (the diameter is 1cm), sterilizing for 3min by using 75% alcohol, washing for 4 times by using sterile water, grinding into homogenate, diluting with sterile water in a gradient manner, dripping the homogenate on an LB (LB) flat plate, culturing overnight at 28 ℃, and counting the number of bacteria.
By continuously spraying the low-concentration betaine solution at 50 μ M to 200 μ M (molar mass concentration, i.e., 5.857 to 23.43mg betaine per liter of water) for the above short time (7 days), it was found that the treated cassava material was more resistant to diseases than the untreated cassava material. As shown in figure 1, when the bacteria survived on the cassava multiply faster and the number of bacteria is more, when the bacteria are infected for 6 days, the log value of the number of the bacteria reaches about 6.9, and the number of the bacteria after being treated by the low-concentration betaine solution of 50-200 mu M (the molar mass concentration is 5.857-23.43mg of betaine contained in each liter of water) reaches about 5.3; that is to say that the bacteria count of the cassava material treated with the low-concentration betaine solution of 50 μ M to 200 μ M (molar mass concentration, i.e. containing 5.857 to 23.43mg betaine per litre of water) is about 3/4 of the bacteria count of the cassava material not treated. Test results show that the betaine spraying treatment can improve the resistance of cassava to bacterial wilt.
Claims (3)
1. A method for improving the bacterial wilt resistance of cassava through betaine induction treatment, wherein the bacteria are xanthomonas carpi cassava wilting pathovar, is characterized by comprising the following steps: continuously spraying betaine solution with the concentration of 50-100 mu M to the leaf surfaces of the cassava seedlings for 7-9 days and 2 times/day, wherein the height of the cassava seedlings when the betaine solution is sprayed is 15-30 cm.
2. The method for improving the bacterial wilt resistance of cassava through betaine induction treatment according to claim 1, which further comprises the steps of fertilizing, deep ploughing, leveling and planting the seedling growing land before seedling growing, and watering at 1-40d after seedling growing; wherein:
the fertilizing step is that enough base fertilizers are applied at one time, including decomposed farmyard manure 2000-;
the planting step adopts a stem cuttage method, the cuttage density is 5 per square meter from early spring to late spring, 2/3 with the length of seed stems and the ground form an angle of 15-45 degrees and are buried in planting ditches, 1/3 of the seed stems expose surface soil, after cuttage, the seeds are pressed to enable the stems to contact with the soil, and the stems are watered thoroughly to keep the soil moist;
watering for 500-; 300-40 d watering, 600L/mu/day, and irrigating by rainwater under natural conditions after 40 days.
3. The method for improving the bacterial wilt resistance of cassava through betaine induction treatment according to claim 2, wherein the cassava is deeply turned over for 15-20 cm after fertilization, and then the seedling land is leveled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810602395.0A CN108848975B (en) | 2018-06-12 | 2018-06-12 | Method for improving cassava disease resistance through betaine treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810602395.0A CN108848975B (en) | 2018-06-12 | 2018-06-12 | Method for improving cassava disease resistance through betaine treatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108848975A CN108848975A (en) | 2018-11-23 |
| CN108848975B true CN108848975B (en) | 2020-11-24 |
Family
ID=64338214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810602395.0A Expired - Fee Related CN108848975B (en) | 2018-06-12 | 2018-06-12 | Method for improving cassava disease resistance through betaine treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108848975B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110024611B (en) * | 2019-05-24 | 2020-12-22 | 海南大学 | Method for improving disease resistance of betel nuts through arecoline treatment and application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0411780A2 (en) * | 1989-07-18 | 1991-02-06 | KAMPEN, Willem Hemmo | Continuous process for the recovery of betaine |
| CN1159740A (en) * | 1994-09-07 | 1997-09-17 | 卡尔特有限公司 | Improving the yield of plants |
| CN104365306A (en) * | 2013-08-13 | 2015-02-25 | 龚圣 | Cassava cultivation and management method |
| CN104429478A (en) * | 2014-11-11 | 2015-03-25 | 黄全 | High-yield cassava cultivation technology |
| CN105076253A (en) * | 2015-08-26 | 2015-11-25 | 山东省农业科学院农业质量标准与检测技术研究所 | Composite growth regulator special for cucumbers |
| CN105409952A (en) * | 2015-11-06 | 2016-03-23 | 浙江大学 | Preparation for reducing damage to corn plants in cadmium pollution environments and method for applying preparation |
-
2018
- 2018-06-12 CN CN201810602395.0A patent/CN108848975B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0411780A2 (en) * | 1989-07-18 | 1991-02-06 | KAMPEN, Willem Hemmo | Continuous process for the recovery of betaine |
| CN1159740A (en) * | 1994-09-07 | 1997-09-17 | 卡尔特有限公司 | Improving the yield of plants |
| CN104365306A (en) * | 2013-08-13 | 2015-02-25 | 龚圣 | Cassava cultivation and management method |
| CN104429478A (en) * | 2014-11-11 | 2015-03-25 | 黄全 | High-yield cassava cultivation technology |
| CN105076253A (en) * | 2015-08-26 | 2015-11-25 | 山东省农业科学院农业质量标准与检测技术研究所 | Composite growth regulator special for cucumbers |
| CN105409952A (en) * | 2015-11-06 | 2016-03-23 | 浙江大学 | Preparation for reducing damage to corn plants in cadmium pollution environments and method for applying preparation |
Non-Patent Citations (4)
| Title |
|---|
| 4种防御酶在木薯-细菌性枯萎病菌反应中的活性变化;樊春俊等;《热带农业科学》;20170531;第32卷(第5期);第1955-1962页 * |
| 徐子健等.甜菜碱参与西瓜抗枯萎病的初步探究.《热带作物学报》.2018,第39卷(第2期),第355-360页. * |
| 木薯抗细菌性枯萎病的生理机制研究;李伯凌;《中国优秀硕士学位论文全文数据库-农业科技辑》;20180215(第2期);第D046-115页 * |
| 甜菜碱参与西瓜抗枯萎病的初步探究;徐子健等;《热带作物学报》;20180228;第39卷(第2期);第355-360页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108848975A (en) | 2018-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104429536B (en) | A kind of cultural method controlling tomato root-knot eelworm | |
| Mostypan et al. | Modern aspects of tilled crops productivity forecasting. | |
| CN102776124A (en) | Compound microbes for reducing and disinfecting greenhouse soil and soil disinfection method | |
| CN105331567B (en) | A kind of bacillus amyloliquefaciens and promoting growth of plants preparation and application | |
| CN103875498A (en) | Method for planting malus halliana | |
| CN107094568B (en) | Blueberry planting method in mild saline-alkali soil | |
| CN110063222A (en) | Overcome the pepper planting method of greenhouse continuous cropping obstacle | |
| CN103329770A (en) | New regulation of blueberry planting technology in subtropical regions | |
| CN105557252B (en) | Medicine and fertilizer integrated pseudo-ginseng cultivation method | |
| CN107182478A (en) | A kind of pseudo-wild cultivating method of sealwort | |
| Bekele | The effect of tillage on soil moisture conservation: A review | |
| CN103444399A (en) | Tobacco cultivating method | |
| CN109874627A (en) | A kind of Spring Peanut Biodegradable mulch trickle irrigation implantation methods | |
| CN108848975B (en) | Method for improving cassava disease resistance through betaine treatment | |
| Rahbarian et al. | Effects of drought stress and manure on dry herb yield and essential oil of dragonhead (Dracocphalum moldavica) in Jiroft erea. | |
| CN105309081B (en) | Soil disinfection method matched with medicinal plant cultivation base | |
| RU2556902C1 (en) | Environmentally friendly method of potato cultivation | |
| CN108218507B (en) | Sugarcane planting method | |
| CN1625936A (en) | Mcthod for rccovering and rebuilding vegetation of Chinese wildrye in soda saline land | |
| CN100557013C (en) | The bacterial strain PX 35 of control curing glasshouse vegetable epidemic disease | |
| CN111727847B (en) | Straw fiber seedling culture substrate for colonization biocontrol bacteria, production method thereof and two-step colonization biocontrol bacteria method | |
| CN110012802A (en) | A kind of continuous cropping implantation methods of Radix Notoginseng | |
| Mkhabela et al. | The effect of mulching on soil moisture retention and yield of lettuce (Lactuca sativa L.) | |
| CN106962011A (en) | A kind of method of microbe planting technology corydalis tuber | |
| CN107041222A (en) | A kind of walnut-wheat integrated cultivation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201124 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |