CN106962011A - A kind of method of microbe planting technology corydalis tuber - Google Patents

A kind of method of microbe planting technology corydalis tuber Download PDF

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Publication number
CN106962011A
CN106962011A CN201710388135.3A CN201710388135A CN106962011A CN 106962011 A CN106962011 A CN 106962011A CN 201710388135 A CN201710388135 A CN 201710388135A CN 106962011 A CN106962011 A CN 106962011A
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corydalis tuber
soil
mus
microbe
planting technology
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祁俊生
周浓
张静
戚特
刘雷
张�杰
陈华华
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Chongqing Three Gorges University
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Chongqing Three Gorges University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

Abstract

A kind of method of microbe planting technology corydalis tuber, comprises the following steps:Using corydalis tuber as material, composite microbic bacterial fertilizer is sowed under the conditions of non-sterile soil, NEB 26, Bai Wo, biological function bacterium, swash antibacterial 968, three torches fill golden liquid, a kind of microbial manure is in soil in mycorhiza, earthing, sow corydalis tuber, earthing, keep soil moisture content 25% 30%, it is placed in potted plant cultivation base, the survival rate and germination percentage of seed can be significantly improved, by the release for reducing corydalis tuber root allelochemical, to adjust corydalis tuber root soil environment, effectively prevent high temperature stress of the corydalis tuber in growth course, lesion, reduce the use of agricultural chemicals, prevent continuous cropping obstacle, it ensure that the security of corydalis tuber, improve the quality and yield of corydalis tuber.

Description

A kind of method of microbe planting technology corydalis tuber
Technical field
The present invention relates to Chinese medicine plantation, specially a kind of method of microbe planting technology corydalis tuber.
Background technology
Corydalis tuber is Papaveraceae Genus Corydalis corydalis tuberCorydalis yanhusuo W. T. Wang dried pieces Stem, is loaded in early days《Bencao Shiyi》, it is one of famous " eight Zhe's ", conventional bulk medicinal materials.Alias:Profound Hu, corydalis tuber.Corydalis tuber Medicinal history is long, cultivation period is short.Research finds, in corydalis tuber benzylisoquinoline type alkaloid be mainly used in analgesia, it is calm, Hypnosis, anti-arrhythmia.In addition, the ucleosides thing such as adenosine, uridine, guanosine, cytidine, thymidine, 2'- desoxyadenossines in corydalis tuber Matter, can antitumor, antiviral, liver protection.Chemical composition is more in corydalis tuber, and clinical practice is wider, and supply falls short of demand.Corydalis tuber system shallow root Crop, it is desirable to loosing soil, fertile.In current corydalis tuber artificial cultivation domestication, generally existing introduces a collection is unclear, variety deterioration, disease The insect pest incidence of disease is high, using serious problems such as traditional cultivation patterns, causes corydalis tuber yield reduction, quality decline, causes continuous cropping Obstacle, significantly limit the sustainable development of corydalis tuber artificial cultivation.
Chinese patent 201410337489.1 and Chinese patent 201310316875.8 disclose a kind of kind of corydalis tuber Plant method, including site preparation, sowing, tillage and weeding, topdress, final-period management, the preventing and treating of pest and disease damage and later stage pick and process, this Two kinds of inventions mainly reach improvement soil compaction phenomenon by laying sowing media etc. on seedbed top layer, improve its loosing soil The content of trace element in degree, retain water and nutrients, increase soil etc., reduces pest and disease damage, promotes corydalis tuber growth, further promotes Yield and quality etc..There is serious continuous cropping obstacle in the cultivation of corydalis tuber.In the production process of corydalis tuber, continuous cropping After cause rhizome rot and blade non-refractory it is very serious, cause invertion rate of green plants to be up to more than 90%, annual 3 to annual high temperature in March The middle ten days and the last ten days moon causes corydalis tuber output and quality seriously to glide on the occasion of corydalis tuber growth and development stage.Clump branch bacterium in microbial-bacterial fertilizer Mycorrhiza fungi can form homobium with more than 80% land plant, can promote the nutrient absorption of plant, improve the resistance of plant. Recently, Mycorrhizal Technology increasingly shows its advantage in organic agriculture, that is, reduces the use of fertilizer and pesticide.To overcome continuous cropping Obstacle, in addition to using measures such as annual crop rotations, can change Soil Micro-environment by microorganism etc. at present, promote beneficial microorganism Development reaches the purpose for overcoming continuous cropping obstacle.Therefore, microbial technique is applied in Chinese medicine cultivation, to high-quality, high yield The Chinese medicine implantation methods of amount, reduce disease and occur, prevent continuous cropping obstacle to have practical significance.
The content of the invention
It is an object of the invention to be the above-mentioned deficiency of solution, it is proposed that one kind overcomes corydalis tuber continuous cropping obstacle, high-yield high-quality Implantation methods, specifically using microorganism plantation corydalis tuber is applied, overcome corydalis tuber continuous cropping obstacle and to obtain high-quality high The corydalis tuber implantation methods of production.
A kind of method of microbe planting technology corydalis tuber:Using corydalis tuber as material, under the conditions of non-sterile soil Sow composite microbic bacterial fertilizer, NEB-26, Bai Wo, biological function bacterium, sharp antibacterial 968, three torches and fill a kind of micro- in golden liquid, mycorhiza Bio-feritlizer is in soil, earthing, sows corydalis tuber, and earthing keeps soil moisture content 25%-30%, is placed in field cultivating base. Further, the soil is that field cultivating base fertile soil and sandy loam are mixed according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.
Further, seed uses bunch planting during sowing, in sowing in annual October, and it is 5 cm or so to set cave spacing, per cave 1 Grain, is covered with thin sandy soil.
Further, the composite microbic bacterial fertilizer is 100 kg/ mus, and NEB-26 is 100 kg/ mus, visits and irrigates as 100 kg/ Mu, biological function bacterium is 100 kg/ mus, and it is 100 kg/ mus to swash antibacterial 968, and it is 100 kg/ mus that three torches, which fill golden liquid, and mycorhiza is 100 Kg/ mus.
Further, eye is upward during sowing.
Technical solutions according to the invention are relative to be had the advantage that:
(1)It is of the present invention cultivation corydalis tuber method without using agriculture chemical in the case of, corydalis tuber seedling can be substantially reduced The pest and disease damage incidence of phase, mitigates continuous cropping obstacle, effectively prevents because of soil hardening that chemical fertilizer is brought etc..
(2)The method of cultivation corydalis tuber of the present invention can remarkably promote corydalis tuber and grow, and increase root yield.
(3)The method of cultivation corydalis tuber of the present invention significantly improves corydalis tuber medical material quanlity.
(4)The method of cultivation corydalis tuber of the present invention significantly reduces making for corydalis tuber cultivation Pesticides chemical fertilizer With, it is ensured that the medical material quanlity of corydalis tuber, reduce the pollution to environment in production planting process.
Embodiment
The present invention is further illustrated with reference to instantiation.
Embodiment 1
A kind of method of microbe planting technology corydalis tuber, comprises the following steps:
(1)Soil is selected, field cultivating base fertile soil is mixed with local sandy loam according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.Seed selection, selection is collected in the corydalis tuber in zhejiang pan'an Xin Wo towns Gu Zhu villages as introduces a collection.
(2)Plantation, is sowed in annual October, potted plant using room temperature(Cultivation basket)Method, microbial bacterial agent is uniform Layer imposes on the lower corydalis tuber root of table soil, comprises the following steps that:By the cm of distance between rows and hills 5 or so density discharge kind block in cultivating bag Stem, upward, seed uses bunch planting to eye, per 1, cave, applies after microbial manure, will break soil into pieces and be covered on seed, and earthing is thick Spend for the cm of 5 cm~6.
Composite microbic bacterial fertilizer is 100 kg/ mus.
(3)In field management, whole planting process, using artificial weeding.
(4)Harvesting, excavation is started at the beginning of 5 months in Second Year, and soil is cleaned.
(5)Preserve, 45 DEG C of drying after cleaning, for attributional analysis.
The experiment such as sample collection is carried out in corydalis tuber growth course, with reference to corydalis tuber single plant yield, Photosynthetic Pigment Content, cell defense enzymes activity in leaves, blade MDA, soluble sugar, soluble protein content, with 6 kinds of biologies in corydalis tuber medicinal material Alkali is Main Analysis object(It is shown in Table 1)Although, it can be seen that Biflorine, hydrochloric acid coptisine, Palmatine Hydro-chloride, hydrochloric acid barberry Alkali, dehydrocorydaline content decreases, but provides corydalis tuber according to Pharmacopoeia of People's Republic of China (version one in 2010) B prime is main Con trolling index, and amplification is 781.30%, and alkaloid total amount also increased.Due to corydalis tuber soil root micro-loop Border is greatly improved, individual plant volume increase 49.14%.
The addition composite microbic bacterial fertilizer corydalis tuber planting effect research of table 1
Embodiment 2
A kind of method of microbe planting technology corydalis tuber, comprises the following steps:
(1)Soil is selected, field cultivating base fertile soil is mixed with local sandy loam according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.Seed selection, selection is collected in the corydalis tuber in zhejiang pan'an Xin Wo towns Gu Zhu villages as introduces a collection.
(2)Plantation, is sowed in annual October, potted plant using room temperature(Cultivation basket)Method, microbial bacterial agent is uniform Layer imposes on the lower corydalis tuber root of table soil, comprises the following steps that:By the cm of distance between rows and hills 5 or so density discharge kind block in cultivating bag Stem, upward, seed uses bunch planting to eye, per 1, cave, applies after microbial manure, will break soil into pieces and be covered on seed, and earthing is thick Spend for the cm of 5 cm~6.
NEB-26 is 100 kg/ mus.
(3)In field management, whole planting process, using artificial weeding.
(4)Harvesting, excavation is started at the beginning of 5 months in Second Year, and soil is cleaned.
(5)Preserve, 45 DEG C of drying after cleaning, for attributional analysis.
The experiment such as sample collection is carried out in corydalis tuber growth course, with reference to corydalis tuber single plant yield, Photosynthetic Pigment Content, cell defense enzymes activity in leaves, blade MDA, soluble sugar, soluble protein content, with 6 kinds of biologies in corydalis tuber medicinal material Alkali is Main Analysis object(It is shown in Table 2)Although, it can be seen that Biflorine, hydrochloric acid coptisine, Palmatine Hydro-chloride, hydrochloric acid barberry Alkali, dehydrocorydaline content decreases, but provides corydalis tuber according to Pharmacopoeia of People's Republic of China (version one in 2010) B prime is main Con trolling index, and amplification is 252.59%.It is single because corydalis tuber soil root microenvironment is greatly improved Strain volume increase 134.19%.
The addition NEB-26 corydalis tubers planting effect research of table 2
Embodiment 3
A kind of method of microbe planting technology corydalis tuber, comprises the following steps:
(1)Soil is selected, field cultivating base fertile soil is mixed with local sandy loam according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.Seed selection, selection is collected in the corydalis tuber in zhejiang pan'an Xin Wo towns Gu Zhu villages as introduces a collection.
(2)Plantation, is sowed in annual October, potted plant using room temperature(Cultivation basket)Method, microbial bacterial agent is uniform Layer imposes on the lower corydalis tuber root of table soil, comprises the following steps that:By the cm of distance between rows and hills 5 or so density discharge kind block in cultivating bag Stem, upward, seed uses bunch planting to eye, per 1, cave, applies after microbial manure, will break soil into pieces and be covered on seed, and earthing is thick Spend for the cm of 5 cm~6.
Bai Wo is 100 kg/ mus.
(3)In field management, whole planting process, using artificial weeding.
(4)Harvesting, excavation is started at the beginning of 5 months in Second Year, and soil is cleaned.
(5)Preserve, 45 DEG C of drying after cleaning, for attributional analysis.
The experiment such as sample collection is carried out in corydalis tuber growth course, with reference to corydalis tuber single plant yield, Photosynthetic Pigment Content, cell defense enzymes activity in leaves, blade MDA, soluble sugar, soluble protein content, with 6 kinds of biologies in corydalis tuber medicinal material Alkali is Main Analysis object(It is shown in Table 3), it can be seen that Biflorine, hydrochloric acid coptisine, Palmatine Hydro-chloride, Berberine hydrochloride, take off Hydrogen corydalis alkali content is increased considerably, and tetrahydropalmatine is provided according to Pharmacopoeia of People's Republic of China (version one in 2010) For main Con trolling index, amplification is 879.32%, and alkaloid total amount also increased.Because corydalis tuber soil root microenvironment is obtained Great improvement, individual plant volume increase 23.86% are arrived.
Fertile corydalis tuber planting effect research is visitd in the addition of table 3
Embodiment 4
A kind of method of microbe planting technology corydalis tuber, comprises the following steps:
(1)Soil is selected, field cultivating base fertile soil is mixed with local sandy loam according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.Seed selection, selection is collected in the corydalis tuber in zhejiang pan'an Xin Wo towns Gu Zhu villages as introduces a collection.
(2)Plantation, is sowed in annual October, potted plant using room temperature(Cultivation basket)Method, microbial bacterial agent is uniform Layer imposes on the lower corydalis tuber root of table soil, comprises the following steps that:By the cm of distance between rows and hills 5 or so density discharge kind block in cultivating bag Stem, upward, seed uses bunch planting to eye, per 1, cave, applies after microbial manure, will break soil into pieces and be covered on seed, and earthing is thick Spend for the cm of 5 cm~6.
Biological function bacterium is 100 kg/ mus.
(3)In field management, whole planting process, using artificial weeding.
(4)Harvesting, excavation is started at the beginning of 5 months in Second Year, and soil is cleaned.
(5)Preserve, 45 DEG C of drying after cleaning, for attributional analysis.
The experiment such as sample collection is carried out in corydalis tuber growth course, with reference to corydalis tuber single plant yield, Photosynthetic Pigment Content, cell defense enzymes activity in leaves, blade MDA, soluble sugar, soluble protein content, with 6 kinds of biologies in corydalis tuber medicinal material Alkali is Main Analysis object(It is shown in Table 4)Although, it can be seen that Biflorine, hydrochloric acid coptisine, Palmatine Hydro-chloride, dehydrocorydaline Content decreases, but provides that tetrahydropalmatine is main control according to Pharmacopoeia of People's Republic of China (version one in 2010) Index processed, amplification is 29.04%, and Berberine hydrochloride also increased.Because corydalis tuber soil root microenvironment has been obtained greatly Improve, individual plant volume increase 148.92%.
The addition biological function bacterium corydalis tuber planting effect research of table 4
Embodiment 5
A kind of method of microbe planting technology corydalis tuber, comprises the following steps:
(1)Soil is selected, field cultivating base fertile soil is mixed with local sandy loam according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.Seed selection, selection is collected in the corydalis tuber in zhejiang pan'an Xin Wo towns Gu Zhu villages as introduces a collection.
(2)Plantation, is sowed in annual October, potted plant using room temperature(Cultivation basket)Method, microbial bacterial agent is uniform Layer imposes on the lower corydalis tuber root of table soil, comprises the following steps that:By the cm of distance between rows and hills 5 or so density discharge kind block in cultivating bag Stem, upward, seed uses bunch planting to eye, per 1, cave, applies after microbial manure, will break soil into pieces and be covered on seed, and earthing is thick Spend for the cm of 5 cm~6.
It is 100 kg/ mus to swash antibacterial 968.
(3)In field management, whole planting process, using artificial weeding.
(4)Harvesting, excavation is started at the beginning of 5 months in Second Year, and soil is cleaned.
(5)Preserve, 45 DEG C of drying after cleaning, for attributional analysis.
The experiment such as sample collection is carried out in corydalis tuber growth course, with reference to corydalis tuber single plant yield, Photosynthetic Pigment Content, cell defense enzymes activity in leaves, blade MDA, soluble sugar, soluble protein content, with 6 kinds of biologies in corydalis tuber medicinal material Alkali is Main Analysis object(It is shown in Table 5)Although, it can be seen that Biflorine, hydrochloric acid coptisine, Palmatine Hydro-chloride, dehydrocorydaline Content decreases, but provides that tetrahydropalmatine is main control according to Pharmacopoeia of People's Republic of China (version one in 2010) Index processed, amplification is 781.30%, and Berberine hydrochloride, alkaloid total amount also increased.Due to corydalis tuber soil root microenvironment It is greatly improved, individual plant volume increase 764.16%.
The addition of table 5 swashs the research of the corydalis tuber planting effect of antibacterial 968
Embodiment 6
A kind of method of microbe planting technology corydalis tuber, comprises the following steps:
(1)Soil is selected, field cultivating base fertile soil is mixed with local sandy loam according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.Seed selection, selection is collected in the corydalis tuber in zhejiang pan'an Xin Wo towns Gu Zhu villages as introduces a collection.
(2)Plantation, is sowed in annual October, potted plant using room temperature(Cultivation basket)Method, microbial bacterial agent is uniform Layer imposes on the lower corydalis tuber root of table soil, comprises the following steps that:By the cm of distance between rows and hills 5 or so density discharge kind block in cultivating bag Stem, upward, seed uses bunch planting to eye, per 1, cave, applies after microbial manure, will break soil into pieces and be covered on seed, and earthing is thick Spend for the cm of 5 cm~6.
It is 100 kg/ mus that three torches, which fill golden liquid,.
(3)In field management, whole planting process, using artificial weeding.
(4)Harvesting, excavation is started at the beginning of 5 months in Second Year, and soil is cleaned.
(5)Preserve, 45 DEG C of drying after cleaning, for attributional analysis.
The experiment such as sample collection is carried out in corydalis tuber growth course, with reference to corydalis tuber single plant yield, Photosynthetic Pigment Content, cell defense enzymes activity in leaves, blade MDA, soluble sugar, soluble protein content, with 6 kinds of biologies in corydalis tuber medicinal material Alkali is Main Analysis object(It is shown in Table 6)Although, it can be seen that Biflorine, hydrochloric acid coptisine, Palmatine Hydro-chloride, hydrochloric acid barberry Alkali, dehydrocorydaline content decreases, but provides corydalis tuber according to Pharmacopoeia of People's Republic of China (version one in 2010) B prime is main Con trolling index, and amplification is 20.13%.Because corydalis tuber soil root microenvironment is greatly improved, individual plant Volume increase 130.98%.
Table 6 adds three torches and fills golden liquid corydalis tuber planting effect research
Embodiment 7
A kind of method of microbe planting technology corydalis tuber, comprises the following steps:
(1)Soil is selected, field cultivating base fertile soil is mixed with local sandy loam according to 3: 1 ratios, air-dries sieving, soil is filled per basin The kg of earth 9.0.Seed selection, selection is collected in the corydalis tuber in zhejiang pan'an Xin Wo towns Gu Zhu villages as introduces a collection.
(2)Plantation, is sowed in annual October, potted plant using room temperature(Cultivation basket)Method, microbial bacterial agent is uniform Layer imposes on the lower corydalis tuber root of table soil, comprises the following steps that:By the cm of distance between rows and hills 5 or so density discharge kind block in cultivating bag Stem, upward, seed uses bunch planting to eye, per 1, cave, applies after microbial manure, will break soil into pieces and be covered on seed, and earthing is thick Spend for the cm of 5 cm~6.
Mycorhiza is 100 kg/ mus.
(3)In field management, whole planting process, using artificial weeding.
(4)Harvesting, excavation is started at the beginning of 5 months in Second Year, and soil is cleaned.
(5)Preserve, 45 DEG C of drying after cleaning, for attributional analysis.
The experiment such as sample collection is carried out in corydalis tuber growth course, with reference to corydalis tuber single plant yield, Photosynthetic Pigment Content, cell defense enzymes activity in leaves, blade MDA, soluble sugar, soluble protein content, with 6 kinds of biologies in corydalis tuber medicinal material Alkali content is Main Analysis object(It is shown in Table 7), it can be seen that although individual plant rate of growth decreases, Biflorine, the hydrochloric acid coptis Alkali, Palmatine Hydro-chloride, Berberine hydrochloride, dehydrocorydaline content is significantly lifted, according to Pharmacopoeia of People's Republic of China (version one in 2010) regulation tetrahydropalmatine is main Con trolling index, and amplification is 963.92%, and alkaloid total amount has also increased Plus.Because corydalis tuber soil root microenvironment is greatly improved, corydalis tuber quality is also greatly improved.
The addition mycorhiza corydalis tuber planting effect research of table 7
Herein it should be noted that, although the present invention has carried out detailed narration to it by above-mentioned specific implementation case, But, those skilled in the art should be understood that any form without departing from claims done on this basis The change of details, belongs to claimed category of the invention.

Claims (5)

1. a kind of method of microbe planting technology corydalis tuber, comprises the following steps:Using corydalis tuber as material, in non-sterilizing Composite microbic bacterial fertilizer, NEB-26, Bai Wo, biological function bacterium, sharp antibacterial 968, three torches are sowed under edaphic condition and fill golden liquid, mycorhiza In a kind of microbial manure in soil, earthing, sow corydalis tuber, earthing, keep soil moisture content 25%-30%, be placed in open country Outer cultivation base.
2. a kind of method of use microbe planting technology corydalis tuber according to claim 1, it is characterised in that:It is described Soil is that field cultivating base fertile soil and sandy loam are mixed according to 3: 1 ratios, air-dries sieving, dress soil 9.0 kg per basin.
3. a kind of method of use microbe planting technology corydalis tuber according to claim 1, it is characterised in that:Sowing When seed use bunch planting, in sowing in annual October, it is 5 cm or so to set cave spacing, per 1, cave, is covered with thin sandy soil.
4. a kind of method of use microbe planting technology corydalis tuber according to claim 1, it is characterised in that:It is described Composite microbic bacterial fertilizer is 100 kg/ mus, and NEB-26 is 100 kg/ mus, visits and irrigates as 100 kg/ mus, biological function bacterium is 100 Kg/ mus, it is 100 kg/ mus to swash antibacterial 968, and it is 100 kg/ mus that three torches, which fill golden liquid, and mycorhiza is 100 kg/ mus.
5. a kind of method of use microbe planting technology corydalis tuber according to claim 1, it is characterised in that:Sowing When eye upward.
CN201710388135.3A 2017-05-27 2017-05-27 A kind of method of microbe planting technology corydalis tuber Pending CN106962011A (en)

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Publication number Priority date Publication date Assignee Title
CN107912251A (en) * 2017-11-10 2018-04-17 蒋世芬 A kind of planting technology of corydalis tuber
CN111713360A (en) * 2020-06-29 2020-09-29 重庆三峡学院 Corydalis tuber planting method

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CN102988494A (en) * 2012-11-20 2013-03-27 亳州市永刚饮片厂有限公司 Production method of rhizoma corydalis medicinal slices
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107912251A (en) * 2017-11-10 2018-04-17 蒋世芬 A kind of planting technology of corydalis tuber
CN111713360A (en) * 2020-06-29 2020-09-29 重庆三峡学院 Corydalis tuber planting method

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