Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a compound fertilizer.
The purpose of the invention is realized by the following technical scheme:
a compound fertilizer is prepared by the following steps:
(1) mixing brown sugar and water according to a solid-to-liquid ratio of 1 g: (15-25) mL, stirring for 8-12 minutes at 60-100 rpm to obtain brown sugar water;
(2) mixing the mixed bacterial powder and water according to a solid-liquid ratio of 1 g: (400-800) mL, stirring for 8-12 minutes at 60-100 rpm to obtain a mixed bacterial liquid, wherein the mixed bacterial liquid is a mixture of 20-40 wt% of bacillus subtilis, 20-40 wt% of bacillus mucilaginosus and 30-50 wt% of aspergillus oryzae;
(3) respectively crushing 20-30 parts of chicken manure, 15-25 parts of rapeseed cakes and 15-25 parts of soybean meal, sieving the crushed materials with a 80-mesh sieve, uniformly mixing the crushed materials, uniformly mixing the uniformly mixed materials with 0.1-1 part of a fermentation accelerator, 0.1-0.5 part of cellulase, 1-3 parts of mixed bacteria liquid and 30-50 parts of brown sugar water, fermenting the uniformly mixed materials at the temperature of 20-30 ℃ for 5-10 days, stirring the uniformly mixed materials once in the morning and at the evening every day, wherein the stirring time is 20-30 minutes each time, and the stirring speed is 40-80 revolutions per minute to obtain a fermented fertilizer;
(4) uniformly mixing the fermented fertilizer with 2-8 parts of urea, 2-8 parts of ammonium dihydrogen phosphate, 2-8 parts of potassium sulfate and 1-3 parts of a synergist to obtain the compound fertilizer.
The parts in the steps are all parts by weight.
The fermentation accelerator is rhamnolipid and/or sodium dodecyl sulfate. Preferably, the fermentation accelerator is a mixture of rhamnolipid and sodium dodecyl sulfate, wherein the mass ratio of the rhamnolipid to the sodium dodecyl sulfate is (8-12): 1.
the synergist is one or more of polyaspartic acid, potassium polyacrylate and sodium alginate. Preferably, the synergist is sodium alginate and/or polyaspartic acid. More preferably, the synergist is a mixture of polyaspartic acid and sodium alginate, and the mass ratio of polyaspartic acid to sodium alginate is (2-6): 1.
preferably, the compound fertilizer is prepared by adopting the following method:
(1) mixing brown sugar and water according to a solid-to-liquid ratio of 1 g: (15-25) mL, stirring for 8-12 minutes at 60-100 rpm to obtain brown sugar water;
(2) mixing the mixed bacterial powder and water according to a solid-liquid ratio of 1 g: (400-800) mL, stirring for 8-12 minutes at 60-100 rpm to obtain a mixed bacterial liquid, wherein the mixed bacterial liquid is a mixture of 20-40 wt% of bacillus subtilis, 20-40 wt% of bacillus mucilaginosus and 30-50 wt% of aspergillus oryzae;
(3) respectively crushing 20-30 parts of chicken manure, 15-25 parts of rapeseed cakes, 15-25 parts of soybean meal and 2-8 parts of traditional Chinese medicine components, sieving the crushed materials through a 80-mesh sieve, uniformly mixing the crushed materials, 0.1-1 part of a fermentation accelerator, 0.1-0.5 part of cellulase, 1-3 parts of mixed bacteria liquid and 30-50 parts of brown sugar water, fermenting the mixture for 5-10 days at the temperature of 20-30 ℃, respectively stirring the mixture once in the morning and at the evening every day for 20-30 minutes at the stirring speed of 40-80 revolutions per minute to obtain a fermented fertilizer;
(4) uniformly mixing the fermented fertilizer with 2-8 parts of urea, 2-8 parts of ammonium dihydrogen phosphate, 2-8 parts of potassium sulfate and 1-3 parts of a synergist to obtain the compound fertilizer.
The traditional Chinese medicine component is alfalfa and/or fructus amomi, and is further preferably prepared by mixing 50-70 wt% of alfalfa and 30-50 wt% of fructus amomi.
The compound fertilizer has the advantages of organic fertilizer and inorganic fertilizer, has complete nutrients, low cost and long fertilizer effect, can effectively improve soil and fertilize soil, has no residue and no pollution, and is suitable for long-term use; the compound fertilizer is especially suitable for the production of green agricultural products, can improve the product yield, the product quality and the stress resistance of crops such as disease resistance, drought resistance, cold resistance, lodging resistance and the like, and can be widely applied to grain crops such as wheat, rice, corn and the like and economic crops such as tomatoes, grapes, melons, sweet potatoes and the like.
Detailed Description
In the present invention, all the equipment and materials are commercially available or commonly used in the art, and the methods in the following examples are conventional in the art unless otherwise specified.
The bacillus subtilis is purchased from China center for industrial microorganism strain preservation management, and has the strain preservation number: CICC 100020 with viable bacteria concentration of 1011CFU/g。
Bacillus mucilaginosus purchased from Jiangsu Green Biotech limited with viable bacteria concentration of 1011CFU/g。
Aspergillus oryzae, purchased from China center for Industrial microbial culture Collection, with the strain preservation number: CICC2038 with viable bacteria concentration of 1011CFU/g。
The sweet potato seedling is a Xinong 431 sweet potato seedling provided by a sale center of Tieren seeds in the Gao city, the stem is 0.7cm thick, and the seedling is 25 cm high.
The brown sugar is industrial brown sugar produced by Suzhou Yonghua chemical Co.
The rapeseed cake is produced by Huaian hongyang agriculture science and technology development limited company.
Bean pulp, provided by mineral processing factories in the treasure of Ling shou county.
The alfalfa is dry alfalfa provided by InXin pasture planting professional Cooperation agency of yellow Ye city.
Fructus Amomi, prebiotics Chinese medicinal decoction pieces.
The chicken manure is natural dry chicken manure provided by Hebei Runlong biological technology limited company.
Urea, produced by Shanxi Yang coal Fengxi fertilizer industry (group) Limited liability company, and the total nitrogen is more than or equal to 46.4 percent.
Ammonium dihydrogen phosphate, produced by chemical science and technology Limited in Jinan Qingtian.
Rhamnolipid, supplied by the specialty chemical company, part number, in bosch, shanxi: 009.
sodium dodecyl sulfate, manufactured by Shandong trimerization chemical science and technology Limited, model number: K12.
cellulase produced by Shandong Baihong New Material Co., Ltd, and enzyme activity of 20000U/g.
Polyaspartic acid, model 109, produced by Wuhan Qianhui bioengineering, Inc.
Potassium polyacrylate, produced by Qingdao head material Co., Ltd, type: SHK 230X.
Sodium alginate, provided by the chemical industry of Jinhaidian (Qingdao) Co., Ltd., implementation standard: GB 1976-80.
The potassium sulfate is agricultural potassium sulfate produced by chemical group limited of the Jinan alliance.
And (3) measuring the SOD activity of the sweet potato: the test was carried out according to the method in Wu Guo Rong et al, fruit superoxide dismutase Activity and Property, Nanjing Master university newspaper (Nature science newspaper), 1994.17 (2).
And (3) soluble sugar content determination: refer to Yaoxin et al, influence of Shiquan on yield and quality of four sweet potato varieties, Shandong academy of agricultural sciences, 2015.47 (10).
Example 1
The planting method of the sweet potatoes rich in SOD comprises the following steps:
(1) preparing a compound fertilizer;
(2) fertilizing and planting ridge forming: applying 1000kg of compound fertilizer to each mu of land in a planting field of Maanshan and county in Anhui province in 4 months and 2 days, deeply ploughing by 35 cm, crushing soil, leveling the land, forming planting ridges with the space between every two adjacent planting ridges being 70 cm, the height of each ridge being 30 cm and the width of the bottom being 35 cm;
(3) planting: 4, 12, planting sweet potato seedlings on the land treated in the step (2), digging planting holes at the tops of planting ridges, watering 600mL of water in each hole, then planting seedlings and covering soil, planting 3500 seedlings in each mu, timely checking survival conditions within 2-7 days after planting, and finding out dead seedlings and replanting strong seedlings;
(4) management: watering 150 kg/mu of sweet potato seedlings every 25 days after the sweet potato seedlings are planted, spraying the nutrient solution for 5-month 7 days, 5-month 27 days and 6-month 7 days after the sweet potato seedlings are planted, and spraying 80kg of nutrient solution for each mu; spraying the nutrient solution on the leaf surfaces once every 9 months and 2 days, and spraying 70kg of nutrient solution per mu;
(5) harvesting: collecting in 2 days after 10 months to obtain sweet potato rich in SOD.
The preparation method of the compound fertilizer comprises the following steps of:
(1) mixing brown sugar and water according to a solid-to-liquid ratio of 1 g: mixing 20mL of the mixture, and stirring the mixture for 10 minutes at a speed of 80 revolutions per minute to obtain brown sugar water;
(2) mixing the mixed bacterial powder and water according to a solid-liquid ratio of 1 g: mixing 600mL of the mixture, and stirring for 10 minutes at a speed of 80 rpm to obtain a mixed bacterial liquid, wherein the mixed bacterial liquid is a mixture of 30 wt% of bacillus subtilis, 30 wt% of bacillus mucilaginosus and 40 wt% of aspergillus oryzae;
(3) respectively crushing 25 parts of chicken manure, 20 parts of rapeseed cakes and 20 parts of soybean meal, sieving the crushed materials with a 80-mesh sieve, uniformly mixing the crushed materials, uniformly mixing the uniformly mixed materials with 0.5 part of a fermentation accelerator, 0.3 part of cellulase, 2 parts of mixed bacteria liquid and 40 parts of brown sugar water, carrying out open fermentation at 25 ℃ for 8 days, stirring the mixture once in the morning and evening every day, wherein the stirring time is 25 minutes each time, and the stirring speed is 60 revolutions per minute, so as to obtain a fermented fertilizer;
(4) and uniformly mixing the fermented fertilizer with 6 parts of urea, 6 parts of ammonium dihydrogen phosphate, 6 parts of potassium sulfate and 2 parts of a synergist to obtain the compound fertilizer.
The preparation method of the nutrient solution comprises the following steps of: 0.3 part of zinc gluconate, 0.05 part of brassin and 100 parts of water are uniformly mixed to obtain the nutrient solution.
The fermentation promoter is rhamnolipid.
The synergist is polyaspartic acid.
Example 2
The planting method of the sweet potatoes rich in SOD comprises the following steps:
(1) preparing a compound fertilizer;
(2) fertilizing and planting ridge forming: applying 1000kg of compound fertilizer to each mu of land in a planting field of Maanshan and county in Anhui province in 4 months and 2 days, deeply ploughing by 35 cm, crushing soil, leveling the land, forming planting ridges with the space between every two adjacent planting ridges being 70 cm, the height of each ridge being 30 cm and the width of the bottom being 35 cm;
(3) planting: 4, 12, planting sweet potato seedlings on the land treated in the step (2), digging planting holes at the tops of planting ridges, watering 600mL of water in each hole, then planting seedlings and covering soil, planting 3500 seedlings in each mu, timely checking survival conditions within 2-7 days after planting, and finding out dead seedlings and replanting strong seedlings;
(4) management: watering 150 kg/mu of sweet potato seedlings every 25 days after the sweet potato seedlings are planted, spraying the nutrient solution for 5-month 7 days, 5-month 27 days and 6-month 7 days after the sweet potato seedlings are planted, and spraying 80kg of nutrient solution for each mu; spraying the nutrient solution on the leaf surfaces once every 9 months and 2 days, and spraying 70kg of nutrient solution per mu;
(5) harvesting: collecting in 2 days after 10 months to obtain sweet potato rich in SOD.
The preparation method of the compound fertilizer comprises the following steps of:
(1) mixing brown sugar and water according to a solid-to-liquid ratio of 1 g: mixing 20mL of the mixture, and stirring the mixture for 10 minutes at a speed of 80 revolutions per minute to obtain brown sugar water;
(2) mixing the mixed bacterial powder and water according to a solid-liquid ratio of 1 g: mixing 600mL of the mixture, and stirring for 10 minutes at a speed of 80 rpm to obtain a mixed bacterial liquid, wherein the mixed bacterial liquid is a mixture of 30 wt% of bacillus subtilis, 30 wt% of bacillus mucilaginosus and 40 wt% of aspergillus oryzae;
(3) respectively crushing 25 parts of chicken manure, 20 parts of rapeseed cakes, 20 parts of soybean meal and 6 parts of traditional Chinese medicine components, sieving the crushed materials with a 80-mesh sieve, uniformly mixing the crushed materials, uniformly mixing the uniformly mixed materials with 0.5 part of fermentation accelerator, 0.3 part of cellulase, 2 parts of mixed bacteria liquid and 40 parts of brown sugar water, carrying out open fermentation for 8 days at 25 ℃, respectively stirring the uniformly mixed materials once in the morning and at night every day, wherein the stirring time is 25 minutes each time, and the stirring speed is 60 revolutions per minute, so as to obtain a fermented fertilizer;
(4) and uniformly mixing the fermented fertilizer with 6 parts of urea, 6 parts of ammonium dihydrogen phosphate, 6 parts of potassium sulfate and 2 parts of a synergist to obtain the compound fertilizer.
The preparation method of the nutrient solution comprises the following steps of: 0.3 part of zinc gluconate, 0.05 part of brassin and 100 parts of water are uniformly mixed to obtain the nutrient solution.
The traditional Chinese medicine components are prepared by mixing 60 wt% of alfalfa and 40 wt% of fructus amomi.
The fermentation promoter is rhamnolipid.
The synergist is polyaspartic acid.
Example 3
Essentially the same as example 2, except that: the synergist is potassium polyacrylate.
Example 4
Essentially the same as example 2, except that: the synergist is sodium alginate.
Example 5
Essentially the same as example 2, except that: the synergist is a mixture of polyaspartic acid and sodium alginate, and the mass ratio of the polyaspartic acid to the sodium alginate is 4: 1.
example 6
The planting method of the sweet potatoes rich in SOD comprises the following steps:
(1) preparing a compound fertilizer;
(2) fertilizing and planting ridge forming: applying 1000kg of compound fertilizer to each mu of land in a planting field of Maanshan and county in Anhui province in 4 months and 2 days, deeply ploughing by 35 cm, crushing soil, leveling the land, forming planting ridges with the space between every two adjacent planting ridges being 70 cm, the height of each ridge being 30 cm and the width of the bottom being 35 cm;
(3) planting: 4, 12, planting sweet potato seedlings on the land treated in the step (2), digging planting holes at the tops of planting ridges, watering 600mL of water in each hole, then planting seedlings and covering soil, planting 3500 seedlings in each mu, timely checking survival conditions within 2-7 days after planting, and finding out dead seedlings and replanting strong seedlings;
(4) management: watering 150 kg/mu of sweet potato seedlings every 25 days after the sweet potato seedlings are planted, spraying the nutrient solution for 5-month 7 days, 5-month 27 days and 6-month 7 days after the sweet potato seedlings are planted, and spraying 80kg of nutrient solution for each mu; spraying the nutrient solution on the leaf surfaces once every 9 months and 2 days, and spraying 70kg of nutrient solution per mu;
(5) harvesting: collecting in 2 days after 10 months to obtain sweet potato rich in SOD.
The preparation method of the compound fertilizer comprises the following steps of:
(1) mixing brown sugar and water according to a solid-to-liquid ratio of 1 g: mixing 20mL of the mixture, and stirring the mixture for 10 minutes at a speed of 80 revolutions per minute to obtain brown sugar water;
(2) mixing the mixed bacterial powder and water according to a solid-liquid ratio of 1 g: mixing 600mL of the mixture, and stirring for 10 minutes at a speed of 80 rpm to obtain a mixed bacterial liquid, wherein the mixed bacterial liquid is a mixture of 30 wt% of bacillus subtilis, 30 wt% of bacillus mucilaginosus and 40 wt% of aspergillus oryzae;
(3) respectively crushing 25 parts of chicken manure, 20 parts of rapeseed cakes, 20 parts of soybean meal and 6 parts of traditional Chinese medicine components, sieving the crushed materials with a 80-mesh sieve, uniformly mixing the crushed materials, uniformly mixing the uniformly mixed materials with 0.5 part of fermentation accelerator, 0.3 part of cellulase, 2 parts of mixed bacteria liquid and 40 parts of brown sugar water, carrying out open fermentation for 8 days at 25 ℃, respectively stirring the uniformly mixed materials once in the morning and at night every day, wherein the stirring time is 25 minutes each time, and the stirring speed is 60 revolutions per minute, so as to obtain a fermented fertilizer;
(4) and uniformly mixing the fermented fertilizer with 6 parts of urea, 6 parts of ammonium dihydrogen phosphate, 6 parts of potassium sulfate and 2 parts of a synergist to obtain the compound fertilizer.
The preparation method of the nutrient solution comprises the following steps of: 0.3 part of zinc gluconate, 0.05 part of brassin and 100 parts of water are uniformly mixed to obtain the nutrient solution.
The traditional Chinese medicine components are prepared by mixing 60 wt% of alfalfa and 40 wt% of fructus amomi.
The fermentation accelerator is sodium dodecyl sulfate.
The synergist is a mixture of polyaspartic acid and sodium alginate, and the mass ratio of the polyaspartic acid to the sodium alginate is 4: 1.
SOD activity of the obtained sweet potato rich in SOD: 37.5IU/g, soluble sugar content: 48.54mg/kg, yield: 3502 kg/mu.
Example 7
The planting method of the sweet potatoes rich in SOD comprises the following steps:
(1) preparing a compound fertilizer;
(2) fertilizing and planting ridge forming: applying 1000kg of compound fertilizer to each mu of land in a planting field of Maanshan and county in Anhui province in 4 months and 2 days, deeply ploughing by 35 cm, crushing soil, leveling the land, forming planting ridges with the space between every two adjacent planting ridges being 70 cm, the height of each ridge being 30 cm and the width of the bottom being 35 cm;
(3) planting: 4, 12, planting sweet potato seedlings on the land treated in the step (2), digging planting holes at the tops of planting ridges, watering 600mL of water in each hole, then planting seedlings and covering soil, planting 3500 seedlings in each mu, timely checking survival conditions within 2-7 days after planting, and finding out dead seedlings and replanting strong seedlings;
(4) management: watering 150 kg/mu of sweet potato seedlings every 25 days after the sweet potato seedlings are planted, spraying the nutrient solution for 5-month 7 days, 5-month 27 days and 6-month 7 days after the sweet potato seedlings are planted, and spraying 80kg of nutrient solution for each mu; spraying the nutrient solution on the leaf surfaces once every 9 months and 2 days, and spraying 70kg of nutrient solution per mu;
(5) harvesting: collecting in 2 days after 10 months to obtain sweet potato rich in SOD.
The preparation method of the compound fertilizer comprises the following steps of:
(1) mixing brown sugar and water according to a solid-to-liquid ratio of 1 g: mixing 20mL of the mixture, and stirring the mixture for 10 minutes at a speed of 80 revolutions per minute to obtain brown sugar water;
(2) mixing the mixed bacterial powder and water according to a solid-liquid ratio of 1 g: mixing 600mL of the mixture, and stirring for 10 minutes at a speed of 80 rpm to obtain a mixed bacterial liquid, wherein the mixed bacterial liquid is a mixture of 30 wt% of bacillus subtilis, 30 wt% of bacillus mucilaginosus and 40 wt% of aspergillus oryzae;
(3) respectively crushing 25 parts of chicken manure, 20 parts of rapeseed cakes, 20 parts of soybean meal and 6 parts of traditional Chinese medicine components, sieving the crushed materials with a 80-mesh sieve, uniformly mixing the crushed materials, uniformly mixing the uniformly mixed materials with 0.5 part of fermentation accelerator, 0.3 part of cellulase, 2 parts of mixed bacteria liquid and 40 parts of brown sugar water, carrying out open fermentation for 8 days at 25 ℃, respectively stirring the uniformly mixed materials once in the morning and at night every day, wherein the stirring time is 25 minutes each time, and the stirring speed is 60 revolutions per minute, so as to obtain a fermented fertilizer;
(4) and uniformly mixing the fermented fertilizer with 6 parts of urea, 6 parts of ammonium dihydrogen phosphate, 6 parts of potassium sulfate and 2 parts of a synergist to obtain the compound fertilizer.
The preparation method of the nutrient solution comprises the following steps of: 0.3 part of zinc gluconate, 0.05 part of brassin and 100 parts of water are uniformly mixed to obtain the nutrient solution.
The traditional Chinese medicine components are prepared by mixing 60 wt% of alfalfa and 40 wt% of fructus amomi.
The fermentation accelerator is a mixture of rhamnolipid and sodium dodecyl sulfate, wherein the mass ratio of the rhamnolipid to the sodium dodecyl sulfate is 9: 1.
the synergist is a mixture of polyaspartic acid and sodium alginate, and the mass ratio of the polyaspartic acid to the sodium alginate is 4: 1.
SOD activity of the obtained sweet potato rich in SOD: 43.8IU/g, soluble sugar content: 54.67mg/kg, yield: 3984 kg/mu.
Test example 1
SOD activities of the SOD enriched sweet potatoes prepared in examples 1-5 were measured, and the results are shown in Table 1.
Table 1: SOD activity test result table of sweet potatoes rich in SOD
|
SOD Activity, IU/g
|
Example 1
|
31.6
|
Example 2
|
34.2
|
Example 3
|
32.6
|
Example 4
|
35.1
|
Example 5
|
38.9 |
Test example 2
The SOD-enriched sweet potatoes prepared in examples 1-5 were tested for their soluble sugar content, and the specific test results are shown in Table 2.
Table 2: test result table for content of soluble sugar in sweet potatoes rich in SOD
|
Soluble sugar content, mg/kg
|
Example 1
|
41.18
|
Example 2
|
44.65
|
Example 3
|
43.38
|
Example 4
|
45.12
|
Example 5
|
49.63 |
Test example 3
The SOD-enriched sweet potatoes of examples 1-5 were tested for their production and the results are shown in Table 3.
Table 3: SOD-rich sweet potato yield test result table
|
Yield, kg/mu
|
Example 1
|
3054
|
Example 2
|
3206
|
Example 3
|
3155
|
Example 4
|
3281
|
Example 5
|
3613 |
Example 2 the compound fertilizer is added with traditional Chinese medicine components, and the SOD activity, soluble sugar content and yield of the sweet potato rich in SOD obtained are all superior to those of example 1. The alfalfa and the fructus amomi contain trace elements required by the growth of the sweet potatoes, are more beneficial to the absorption of the sweet potatoes through microbial fermentation, effectively promote the growth of the sweet potatoes, and simultaneously supplement the trace elements by adopting the traditional Chinese medicine components, thereby avoiding the environmental pollution caused by direct fertilization by adopting chemical substances.
The embodiment 2-4 optimizes the synergist in the compound fertilizer, the effect of adopting sodium alginate is better, and the chelation of the sodium alginate can effectively promote the absorption of nutrients by the sweet potatoes, improve photosynthesis and promote the growth of the sweet potatoes.
Test example 4
The compound fertilizers prepared in the examples 1, 2, 4, 5, 6 and 7 were subjected to a potting experiment test of wheat by the method disclosed in the example 1 of the chinese patent CN 102584448B. The specific test results are shown in table 4.
Table 4: test result table
|
Average plant height, cm
|
Example 1
|
26.4
|
Example 2
|
28.9
|
Example 4
|
29.5
|
Example 5
|
32.6
|
Example 6
|
33.1
|
Example 7
|
35.6 |
Therefore, the compound fertilizer can also obviously improve the plant height of wheat in the jointing stage.