CN108401896A - One kind overcoming the incompatible method of capsicum distant hybridization - Google Patents

Abstract

The invention discloses one kind overcoming the incompatible method of capsicum distant hybridization, includes the following steps：Step 1：It is spare that 2,4 D 30mg/l and GA3 20mg/l are prepared respectively；Step 2:In the first tenday period of a month in May in a certain fine day proxima luce (prox. luc) afternoon is chosen, the flower that picking C.frutescens capsicums have just opened does not have the anther of loose powder to carry out and stores into cold compartment of refrigerator with tweezers taking-up；Step 3：Morning next day is female parent with C.annuum capsicums, using C.frutescens capsicums as male parent；Four door socket of C.annuum capsicums big flower bud age is selected to spend artificial removal's anther；Step 4：Using C.frutescens capsicum pollen mixtures, the saturation pollination of C.annuum capsicum column caps is uniformly smeared；Step 5：2,4 D 30mg/l solution are dipped with writing brush immediately after pollination, are gently applied on bennet；GA3 20mg/l are dipped with an other writing brush, are gently applied on style；Step 6：Cenospecies is harvested after above-mentioned C.annuum capsicums fruit natural aging；The success rate of Capsicum interspecific hybridization can be improved in the present invention, improves existing pepper breeding.

Description

One kind overcoming the incompatible method of capsicum distant hybridization

Technical field

This application involves technical field of agricultural cultivation more particularly to one kind overcoming the incompatible method of capsicum distant hybridization.

Background technology

Capsicum is a kind of hereditary and abundant vegetable crop of Phenotypic Diversity, and Capsicum (Capsicum spp.) includes 5 A cultigen (C.annuum, C.chinense, C.baccatum, C.frutescens and C.pubescens), C.annuum It is that type is most in the world, cultivates widest kind, pepper breeding is mainly on annual this cultigen of capsicum C.annuum Expansion, but due to long-term higher artificial selection pressure, C.annuum hereditary basis is gradually narrow.C.frutescens etc. has Many excellent characters are the valuable sources for improveing C.annuum.Interspecific hybridization is to realize the effective way of gene transfer, but capsicum Belong to interspecific hybridization there are crossing barrier, as pollen cannot on column cap sprouting and pollen tube growth slowly fail to award Essence, Hybrids F1 are often difficult to obtain.

Invention content

Purpose of the present invention is to：One kind is provided and overcomes the incompatible method of capsicum distant hybridization, Capsicum inter-species can be improved The success rate of distant hybridization improves existing pepper breeding.

The technical scheme is that：One kind overcoming the incompatible method of capsicum distant hybridization, includes the following steps：

Step 1：2,4-D 30mg/l and GA3 are prepared respectively（Gibberellin）20mg/l is spare；

Step 2:In the first tenday period of a month in May in a certain fine day proxima luce (prox. luc) afternoon is chosen, the flower that picking C.frutescens capsicums have just opened is used Tweezers taking-up there is not the anther of loose powder to be placed on smooth blank sheet of paper, and blank sheet of paper is placed in drier and is dried with drier, dry Device is placed on cold compartment of refrigerator preservation；

Step 3：Morning next day is female parent with C.annuum capsicums, using C.frutescens capsicums as male parent；Select C.annuum The big flower bud age flower of four door socket of capsicum（The same day is flower bud, and next day opens, and corolla is considerably longer than calyx, and corolla apex not yet expands, corolla It is white）Artificial removal's anther；And before artificial hybridization, the pollen mixture obtained by step 2 is taken to be packed into pollination tube with pollen sieve sieve It is interior spare；

Step 4：Using C.frutescens capsicum pollen mixtures, the saturation pollination of C.annuum capsicum column caps is uniformly smeared；

Step 5：2,4-D 30mg/l solution is dipped with writing brush immediately after pollination, is gently applied on bennet；With an other hair Pen dips GA3 20mg/l, is gently applied on style；Label is hung up, is marked；

Step 6：Cenospecies is harvested after above-mentioned C.annuum capsicums fruit natural aging.

It is an advantage of the invention that：The exogenous GA 3 of debita spissitudo can promote sprouting and flower of the pollen on column cap in the present invention Tube cell is grown in style, smoothly completes Process of Insemination to obtain Hybrids F1 seed；And the external source 2,4-D of debita spissitudo, energy Fruit drop is reduced, more Hybrids F1 seed is obtained；The success rate of Capsicum interspecific hybridization can be improved in the present invention, improvement Existing pepper breeding.

Specific implementation mode

Embodiment：One kind overcoming the incompatible method of capsicum distant hybridization, includes the following steps：

Step 1：2,4-D 30mg/l and GA3 are prepared respectively（Gibberellin）20mg/l is spare；

Step 2:In the first tenday period of a month in May in a certain fine day proxima luce (prox. luc) afternoon is chosen, the flower that picking C.frutescens capsicums have just opened is used Tweezers taking-up there is not the anther of loose powder to be placed on smooth blank sheet of paper, and blank sheet of paper is placed in drier and is dried with drier, dry Device is placed on cold compartment of refrigerator preservation；

Step 3：Morning next day is female parent with C.annuum capsicums, using C.frutescens capsicums as male parent；Select C.annuum The big flower bud age flower of four door socket of capsicum（The same day is flower bud, and next day opens, and corolla is considerably longer than calyx, and corolla apex not yet expands, corolla It is white）Artificial removal's anther；And before artificial hybridization, the pollen mixture obtained by step 2 is taken to be packed into pollination tube with pollen sieve sieve It is interior spare；

Step 4：Using C.frutescens capsicum pollen mixtures, the saturation pollination of C.annuum capsicum column caps is uniformly smeared；

Step 5：2,4-D 30mg/l solution is dipped with writing brush immediately after pollination, is gently applied on bennet；With an other hair Pen dips GA3 20mg/l, is gently applied on style；Label is hung up, is marked；

Step 6：Cenospecies is harvested after above-mentioned C.annuum capsicums fruit natural aging.

The exogenous GA 3 of debita spissitudo can promote sprouting and pollen tube of the pollen on column cap raw in style in the present invention It is long, Process of Insemination is smoothly completed to obtain Hybrids F1 seed；And the external source 2,4-D of debita spissitudo, fruit drop can be reduced, is obtained Obtain more Hybrids F1 seed；The success rate of Capsicum interspecific hybridization can be improved in the present invention, improves existing pepper breeding.

The specific application example that the above is only the present invention, is not limited in any way protection scope of the present invention.Except above-mentioned Outside embodiment, the present invention can also have other embodiment.All technical solutions formed using equivalent substitution or equivalent transformation, It falls within scope of the present invention.

Claims (1)

1. one kind overcoming the incompatible method of capsicum distant hybridization, it is characterised in that include the following steps：

Step 1：2,4-D 30mg/l and GA3 are prepared respectively（Gibberellin）20mg/l is spare；

Step 2:In the first tenday period of a month in May in a certain fine day proxima luce (prox. luc) afternoon is chosen, the flower that picking C.frutescens capsicums have just opened is used Tweezers taking-up there is not the anther of loose powder to be placed on smooth blank sheet of paper, and blank sheet of paper is placed in drier and is dried with drier, dry Device is placed on cold compartment of refrigerator preservation；

Step 3：Morning next day is female parent with C.annuum capsicums, using C.frutescens capsicums as male parent；Select C.annuum The big flower bud age flower of four door socket of capsicum（The same day is flower bud, and next day opens, and corolla is considerably longer than calyx, and corolla apex not yet expands, corolla It is white）Artificial removal's anther；And before artificial hybridization, the pollen mixture obtained by step 2 is taken to be packed into pollination tube with pollen sieve sieve It is interior spare；

Step 4：Using C.frutescens capsicum pollen mixtures, the saturation pollination of C.annuum capsicum column caps is uniformly smeared；

Step 5：2,4-D 30mg/l solution is dipped with writing brush immediately after pollination, is gently applied on bennet；With an other hair Pen dips GA3 20mg/l, is gently applied on style；Label is hung up, is marked；

Step 6：Cenospecies is harvested after above-mentioned C.annuum capsicums fruit natural aging.