CN106034873A - Seed preparation method for cucumber net room - Google Patents

Seed preparation method for cucumber net room Download PDF

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Publication number
CN106034873A
CN106034873A CN201610361052.0A CN201610361052A CN106034873A CN 106034873 A CN106034873 A CN 106034873A CN 201610361052 A CN201610361052 A CN 201610361052A CN 106034873 A CN106034873 A CN 106034873A
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Prior art keywords
flower
female
melon
male
parent
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Inventor
桑毅振
王明钦
桑松龄
桑毅冲
丁卓
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SHOUGUANG NEW CENTURY SEED CO Ltd
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SHOUGUANG NEW CENTURY SEED CO Ltd
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Priority to CN201610361052.0A priority Critical patent/CN106034873A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protection of plants
    • A01G13/10Devices for affording protection against animals, birds or other pests
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M29/00Scaring or repelling devices, e.g. bird-scaring apparatus
    • A01M29/30Scaring or repelling devices, e.g. bird-scaring apparatus preventing or obstructing access or passage, e.g. by means of barriers, spikes, cords, obstacles or sprinkled water
    • A01M29/34Scaring or repelling devices, e.g. bird-scaring apparatus preventing or obstructing access or passage, e.g. by means of barriers, spikes, cords, obstacles or sprinkled water specially adapted for insects

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pest Control & Pesticides (AREA)
  • Developmental Biology & Embryology (AREA)
  • Insects & Arthropods (AREA)
  • Botany (AREA)
  • Birds (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a seed preparation method for a cucumber net room. The method comprises following steps: setting an improved female-male parent mode in the net room to achieve zonal relay intercropping and planting of female-male parents on a double-concave structure, wherein male parents do not additionally occupy land so that the purpose of increasing the number of female parents and improving growth environment for male and female parents on the total area for seed production land. The yield and quality of hybrid seeds for cucumbers are favorably improved. The yield of hybrid seeds of cucumbers is increased by 25.9% to 27.8% and the thousand seed weight is increased by 1.7 to 1.8g.

Description

A kind of Fructus Cucumidis sativi solarium producing method for seed
Technical field
The present invention relates to a kind of Fructus Cucumidis sativi solarium producing method for seed, belong to vegetable breeding technical field.
Background technology
Hybrid seed production of cucumber is for the purpose of producing high-quality hybrid seed, and the advantage utilizing Fructus Cucumidis sativi male parent maternal shows Property, by producing in yielding ability, disease resistance, resistance the Hybrid significantly more than parents after the artificial hybridization production of hybrid seeds.Yellow The problems such as it is on the low side that melon exists yield during hybrid seeding, and seed quality and purity are difficult to effectively control, and production cost is high, These problems constrain the development of seed of Fructus Cucumidis sativi industry to a certain extent.
The technological deficiency of the Fructus Cucumidis sativi tradition conventional hybridization production of hybrid seeds is mainly manifested in:
(1) planting in the male parent of Fructus Cucumidis sativi, maternal the most individually partition set, male parent takies independent land area, reduces the production of hybrid seeds Plant number maternal on the gross area of plot, causes hybrid seed to yield poorly.
(2) in order to reduce male parent floor space, generally use the method plantation male parent strengthening planting density, cause male parent to plant Disorderly competition between strain, makes the male flower of plant can not get more preferable nutritional condition, and it is poor that male flower is grown, and pollen quantity reduces, unfavorable Adopt powder in carrying out, cause hybridization pollination of poor quality.
(3) roguing opportunity and method can not science, Parent hybrid strain removes thorough, causes the generation of pseudostationary, hybridization Seed purity declines.
(4) must arrange remote isolation area, multiple cucumber hybrids can not be at same base closely breeding, breeding cost Become big.
Therefore, how to improve the systematic seeding technique of Fructus Cucumidis sativi, reduce the generation of pseudostationary, improve hybrid seed yield, fall Low breeding cost is the problem demanding prompt solution that the industry is faced.
Summary of the invention
The technical problem to be solved is for problem above, it is provided that a kind of Fructus Cucumidis sativi solarium producing method for seed, the party It is high that method adds the planting of maternal strain number on the gross area of production of hybrid seeds plot, seed purity and percentage of seedgermination;Improve Parent kind Plant ratio, reduce male parent planting density, be conducive to improving hybrid seed yield and quality, reduce breeding cost.
For solving above technical problem, the Fructus Cucumidis sativi solarium producing method for seed of the present invention, comprise the following steps:
1) solarium is set: select the rich soil that high-land, irrigation and drainage are good, and the plot without quarantine disease sets up arc Frame, span 6-8m, rise 2.5-3.0m, long 60-80m, arc bow member overlying lid 40-50 mesh fly net;
2) cultivation ridge is set: arranging cultivation ridge in solarium, cultivation ridge is double concave type structure, is protruded band and 2 hollow zones by 3 Composition, 3 protrude bandwidth and are 20cm, protrude band height 20cm, and 2 depression bandwidth are 15cm, the deep 20cm of hollow zone, and adjacent 2 Bar cultivation distance between the ridges 130cm;
3) use solarium in Shandong be April early and middle ten days carry out Cucumber Seed Production, the ratio of Parent is 1:7, and male parent is than maternal early sowing Kind 5-7 days, the seedling age phase is about 20 days, and rice shoot reaches to carry out field planting during 3 leaf 1 heart, and male parent field planting is being cultivated the convex of centre, ridge Go out on band, maternal field planting on 2 protrusion bands of cultivation both sides, ridge, the typical case such as leaf, leaf color, growing way according to young plant before field planting Character pair father, female parent carry out the 1st time and go hybrid strain, the young plant of hybrid strain and doubtful hybrid strain are removed, no longer field planting;
4) female parent is hung climing cultivation, when maternal 1st female flower is buddingged, according to typical case's character such as beginning Hua Jiewei, alabastrum color Female parent is carried out the 2nd time and goes hybrid strain;By the 1st female flower pollination self of female parent, sit melon, it is simple to go hybrid strain the 3rd time;Maternal 1st Female flower is sat after melon 8-10 days, carries out the 3rd time going hybrid strain according to typical case's character pair female parent such as curcumbitate, melon skin color, goes hybrid strain the 3rd time Rear in time by female parent sugar-preserved gourd and the 2nd female flower alabastrum excision;Hybridization pollination is started from the 3rd maternal female flower;
5) except male flower: front 1 afternoon of pollinating extracts maternal male flower and male flower alabastrum, it is ensured that maternal during pollination do not have open hero Flower;
6) folder flower: the male flower clip of the 2nd day maternal female flower to be opened and male parent is clamped by front 1 afternoon of pollinating;
7) pollination: when rear 2nd day 8-11 in the morning spent by folder, taken off by the female clip taken, opens petal and exposes stigma, plucks folder flower Male parent male flower, remove petal and expose flower pesticide, paternal pollen is evenly coated on female chapiter and hybridizes, the most again with folder The petal of female flower is clamped by son;
8), after all pollination terminates, paternal plant is pulled out;Pluck fruit, flower, alabastrum and the side of having no result do not pollinated on maternal plant Branch, stays 2-3 sheet leaf pinching in last hybridization side of taking;
9), when hybridization melon length is to 3-4cm size, selects fruit shape 5-6 melon good, supermature to make kind of a melon, unnecessary melon is removed; Kind melon growth course carries out the 4th to female parent and goes hybrid strain, the female parent that kind of melon does not meets varietal character is removed in time;Plant melon to become Gather in time after ripe, after carrying out after-ripening in 7-10 days, take seed.
A kind of prioritization scheme, in described step 3) during field planting, female parent strain is away from for 30cm, and male parent spacing in the rows is 105cm.
Another kind of prioritization scheme, in described step 7), a strain retains 7-8 hybridization female flower, and namely a strain female parent ensures have 7-8 female flower hybridization pollination.
Another prioritization scheme, also hangs climing cultivation to male parent in described step 4), the 1st female flower alabastrum occurs in paternal plant After, carry out male parent according to typical case's character such as beginning Hua Jiewei, alabastrum color and go hybrid strain for the 2nd time, and by the 1st female flower pollination self, Sitting melon, the 1st female flower is sat after melon 8-10 days, carries out the 3rd time going hybrid strain according to typical case's character pair male parent such as curcumbitate, melon skin color, Extract after 3rd time is gone hybrid strain and in time the 1st melon that female flower is sat of male parent.
Use above technical scheme, compared with prior art, have the advantage that
(1) Cucumber Seed Production of the present invention is by innovation cultivation ridge structure in solarium, changes Parent under tradition normal condition and independently divides District's nonoculture plantation is mixing point band relay interplanting planting, the not extra individually land occupation of male parent, adds on the gross area of production of hybrid seeds plot Planting of maternal strain number, improves cucumber hybrid suboutput.667m of the present invention2The more traditional conventional method of hybrid seed yield improves 25.9%-27.8%。
(2) during Cucumber Seed Production of the present invention changes male parent partition set, dense planting is a point band dispersion thin planting, male parent robust growth, forms hero Quantity is many, quality good for flower;Meanwhile, be conducive to improving Parent planting proportion, be effectively improved the strain number of male parent, improve flower The quality of powder and supply, improve the quality of hybridization pollination.The more traditional conventional method of the sub-mass of 1000 kernel of cucumber hybrid of the present invention increases Adding 1.7-1.8g, seed is fuller.
(3) Cucumber Seed Production of the present invention has innovated the method by staying fruit to identify hybrid strain, it is possible to thoroughly remove Parent hybrid strain, Substantially increasing the purity of hybrid seed, seed purity reaches 100%;Percentage of seedgermination is 98.9%-100%, and field goes out without false hybrid strain Existing.
(4) present invention uses solarium's production of hybrid seeds, need not arrange remote isolation area, can realize the same base of multiple cucumber hybrid Ground closely breeding.
Below in conjunction with detailed description of the invention, the invention will be further described.
Detailed description of the invention
The present invention is further illustrated for following example, but is not limited to protection scope of the present invention.
Embodiment 1, field test is in vegetable breeding base, Shouguang City.Cucumber variety is the one of company of Shouguang City selection-breeding For cenospecies Xinjin 11, male parent is xsj05-16, and female parent is xsj05-02.
Test method: test has set 2 contrasts, for the conventional producing method for seed of tradition and producing method for seed of the present invention.
Solarium of the present invention producing method for seed step:
1) solarium is set: select the rich soil that high-land, irrigation and drainage are good, and the plot without quarantine disease sets up arc Frame, span 6-8m, rise 2.5-3.0m, long 60-80m, arc bow member overlying lid 40-50 mesh fly net.
2) cultivation ridge is set: arranging cultivation ridge in solarium, cultivation ridge is double concave type structure, recessed by 3 protrusion bands and 2 Falling into band composition, 3 protrude bandwidth and are 20cm, protrude band height 20cm, and 2 depression bandwidth are 15cm, the deep 20cm of hollow zone, phase Adjacent 2 cultivation distance between the ridges 130cm.
3) use solarium in Shandong be April early and middle ten days carry out Xinjin 11 Cucumber Seed Production, the ratio of Parent is 1:7, father This was than maternal planting early enough 5-7 days, and the seedling age phase is about 20 days, and rice shoot reaches to carry out field planting during 3 leaf 1 heart, male parent field planting was being planted On protrusion band in the middle of banking up, spacing in the rows is 105cm;Maternal field planting is on 2 protrusion bands of cultivation both sides, ridge, and spacing in the rows is 30cm; Before field planting, typical case character pair father, the female parent such as leaf, leaf color, growing way according to young plant carry out the 1st time going hybrid strain, hybrid strain and doubtful The young plant of hybrid strain is removed, no longer field planting.
4) female parent is hung climing cultivation, when maternal 1st female flower is buddingged, according to typical cases such as beginning Hua Jiewei, alabastrum colors Character pair female parent carries out the 2nd time and goes hybrid strain;By the 1st female flower pollination self of female parent, sit melon, it is simple to go hybrid strain the 3rd time;Maternal the 1 female flower is sat after melon 8-10 days, carries out the 3rd time going hybrid strain according to typical case's character pair female parent such as curcumbitate, melon skin color, goes for the 3rd time In time female parent sugar-preserved gourd and the 2nd female flower alabastrum are extractd after hybrid strain;Hybridization pollination is started from the 3rd maternal female flower.Male parent is hung Climing cultivation, after the 1st female flower alabastrum occurs in paternal plant, is carried out according to typical case's character pair male parent such as beginning Hua Jiewei, alabastrum color Go hybrid strain for 2nd time, and by the 1st female flower pollination self, sit melon, the 1st female flower is sat after melon 8-10 days, according to curcumbitate, melon skin face Typical case's character pair male parent such as color carries out the 3rd time and goes hybrid strain, after the 3rd time is gone hybrid strain and extract what the 1st female flower of male parent was sat in time Melon.
5) except male flower: front 1 afternoon of pollinating extracts maternal male flower and male flower alabastrum, it is ensured that maternal the most open during pollination Male flower;
6) folder flower: the male flower clip of the 2nd day maternal female flower to be opened and male parent is clamped by front 1 afternoon of pollinating;
7) pollination: when rear 2nd day 8-11 in the morning spent by folder, taken off by the female clip taken, opens petal and exposes stigma, plucks folder flower Male parent male flower, remove petal and expose flower pesticide, paternal pollen is evenly coated on female chapiter and hybridizes, the most again with folder The petal of female flower is clamped by son.One strain female parent retains 7-8 hybridization female flower.
8), after all pollination terminates, paternal plant is pulled out;Pluck fruit, flower, alabastrum and the nothing do not pollinated on maternal plant Really side shoot, stays 2-3 sheet leaf pinching in last hybridization side of taking.
9), when hybridization melon length is to 3-4cm size, selects fruit shape 5-6 melon good, supermature to make kind of a melon, unnecessary melon is gone Fall;Kind melon growth course carries out the 4th to female parent and goes hybrid strain, kind of melon is not met the maternal timely of No. 11 varietal characters in Xinjin Remove, gather in time after planting melon maturation, after carrying out after-ripening in 7-10 days, take seed.
Above-mentioned go hybrid strain process, be intended to pull out the hybrid strain in male parent, planting of maternal row and doubtful hybrid strain.
Result of the test: present invention producing method for seed 667m more conventional than tradition2Xsj05-02(is maternal) to increase plant number be 435 Strain, Xinjin 11 sub-667m of cucumber hybrid2Output increased 25.9%, single melon amount of setting seeds increases by 1.6, and mass of 1000 kernel increases 1.7g.
From the present invention and the made cenospecies of tradition routine producing method for seed, randomly draw 3000 seeds respectively, carry out Xinjin No. 11 field planting tests, production of hybrid seeds Xinjin of the present invention 11 percentage of seedgermination is 98.9%, and field occurs without false hybrid strain, and seed is pure Degree reaches 100%;Tradition conventional production of hybrid seeds Xinjin 11 percentage of seedgermination is 90.4%, and field has the false hybrid strain of 0.26% to occur.
Embodiment 2, field test is in vegetable breeding base, Shouguang City.Cucumber variety is the one of company of Shouguang City selection-breeding For cenospecies Xinjin 12, male parent is xsj05-18, and female parent is xsj05-06.
Test method: test has set 2 contrasts, for the conventional producing method for seed of tradition and producing method for seed of the present invention.
Solarium of the present invention producing method for seed step: in addition to kind difference, other measure is with embodiment 1.
Result of the test: present invention producing method for seed 667m more conventional than tradition2Xsj05-06(is maternal) increase plant number and be 436 strains, Xinjin 12 sub-667m of cucumber hybrid2Output increased 27.8%, single melon amount of setting seeds increases by 1.7, and mass of 1000 kernel increases 1.8g。
From the present invention and the made cenospecies of tradition routine producing method for seed, randomly draw 3000 seeds respectively, carry out Xinjin No. 12 field planting tests, production of hybrid seeds Xinjin of the present invention 12 percentage of seedgermination is 100%, and field occurs without false hybrid strain, seed purity Reach 100%;Tradition conventional production of hybrid seeds Xinjin 12 percentage of seedgermination is 91.3%, and field has the false hybrid strain of 0.24% to occur.
By the experimental result of above example, it can be seen that take the present invention to carry out Cucumber Seed Production, total face, production of hybrid seeds plot Long-pending upper female parent strain number increases, and paternal pollen is grown, maternal stalwartness of growing, and single melon amount of setting seeds dramatically increases, and mass of 1000 kernel substantially increases Adding, seed is full, and germination percentage is high, and seed purity is high, and yield is high.
The above is the citing of best mode for carrying out the invention, and it is common that the part addressed the most in detail is this area The common knowledge of technical staff.Protection scope of the present invention is as the criterion with the content of claim, any technology based on the present invention The equivalent transformation enlightened and carry out, also within protection scope of the present invention.

Claims (4)

1. a Fructus Cucumidis sativi solarium producing method for seed, it is characterised in that comprise the steps:
1) solarium is set: select the rich soil that high-land, irrigation and drainage are good, and the plot without quarantine disease sets up arc Frame, span 6-8m, rise 2.5-3.0m, long 60-80m, arc bow member overlying lid 40-50 mesh fly net;
2) cultivation ridge is set: arranging cultivation ridge in solarium, cultivation ridge is double concave type structure, is protruded band and 2 hollow zones by 3 Composition, 3 protrude bandwidth and are 20cm, protrude band height 20cm, and 2 depression bandwidth are 15cm, the deep 20cm of hollow zone, and adjacent 2 Bar cultivation distance between the ridges 130cm;
3) use solarium in Shandong be April early and middle ten days carry out Cucumber Seed Production, the ratio of Parent is 1:7, and male parent is than maternal early sowing Kind 5-7 days, the seedling age phase is about 20 days, and rice shoot reaches to carry out field planting during 3 leaf 1 heart, and male parent field planting is being cultivated the convex of centre, ridge Going out on band, maternal field planting, on 2 protrusion bands of cultivation both sides, ridge, carries out the 1st time go hybrid strain to father, female parent before field planting;
4) female parent hanging climing cultivation, the 1st female flower carries out the 2nd time when buddingging and goes hybrid strain, by the 1st female flower pollination self, Sitting melon, the 1st female flower carries out the 3rd time for 8-10 days after sitting melon and goes hybrid strain, goes for the 3rd time after hybrid strain in time by female parent sugar-preserved gourd and the 2nd Female flower alabastrum is extractd, and starts hybridization pollination from the 3rd female flower;
5) except male flower: front 1 afternoon of pollinating extracts maternal male flower and male flower alabastrum, it is ensured that maternal during pollination do not have open hero Flower;
6) folder flower: the male flower clip of the 2nd day maternal female flower to be opened and male parent is clamped by front 1 afternoon of pollinating;
7) pollination: when rear 2nd day 8-11 in the morning spent by folder, taken off by the female clip taken, opens petal and exposes stigma, plucks folder flower Male parent male flower, remove petal and expose flower pesticide, pollen is evenly coated on female chapiter and hybridizes, the most again with clip will The petal of female flower is clamped;
8), after all pollination terminates, paternal plant is pulled out;Pluck fruit, flower, alabastrum and the side of having no result do not pollinated on maternal plant Branch, stays 2-3 sheet leaf pinching in last hybridization side of taking;
9), when hybridization melon length is to 3-4cm size, selects fruit shape 5-6 melon good, supermature to make kind of a melon, unnecessary melon is removed; Kind melon growth course carries out the 4th to female parent and goes hybrid strain;Gather in time after planting melon maturation, after carrying out after-ripening in 7-10 days, take kind Son.
A kind of Fructus Cucumidis sativi solarium the most according to claim 1 producing method for seed, it is characterised in that: in step 3) during field planting, maternal Spacing in the rows is 30cm, and male parent spacing in the rows is 105cm.
A kind of Fructus Cucumidis sativi solarium the most according to claim 1 producing method for seed, it is characterised in that: in step 7), a strain retains 7-8 Piece hybridization female flower, namely a strain female parent has 7-8 colored hybridization pollination.
A kind of Fructus Cucumidis sativi solarium the most according to claim 1 producing method for seed, it is characterised in that: male parent is also hung by step 4) climing Cultivation, plant carries out the 2nd time to male parent after there is the 1st female flower alabastrum and goes hybrid strain, by the 1st female flower pollination self, sits melon, the 1 female flower carries out the 3rd time to male parent in 8-10 days after sitting melon and goes hybrid strain, is extractd by melon in time after going hybrid strain for the 3rd time.
CN201610361052.0A 2016-05-29 2016-05-29 Seed preparation method for cucumber net room Pending CN106034873A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108552051A (en) * 2018-05-02 2018-09-21 张掖市农业科学研究院 A kind of method of solarium's hybrid seed production of cucumber
CN109964809A (en) * 2019-04-22 2019-07-05 广水实实在在生态农业发展有限公司 A kind of method of greenhouse cucumber pollination hybridization

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CN104429536A (en) * 2014-12-08 2015-03-25 寿光市新世纪种苗有限公司 Cultivation method capable of controlling tomato root knot nematode

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108552051A (en) * 2018-05-02 2018-09-21 张掖市农业科学研究院 A kind of method of solarium's hybrid seed production of cucumber
CN109964809A (en) * 2019-04-22 2019-07-05 广水实实在在生态农业发展有限公司 A kind of method of greenhouse cucumber pollination hybridization

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Application publication date: 20161026