CN112243858A - Selfing pollination method for hot peppers and application of selfing pollination method - Google Patents

Selfing pollination method for hot peppers and application of selfing pollination method Download PDF

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Publication number
CN112243858A
CN112243858A CN202011222403.2A CN202011222403A CN112243858A CN 112243858 A CN112243858 A CN 112243858A CN 202011222403 A CN202011222403 A CN 202011222403A CN 112243858 A CN112243858 A CN 112243858A
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China
Prior art keywords
pepper
pollination
self
sleeve
flower
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Inventor
王飞
姚明华
李宁
尹延旭
肖明兆
高升华
余楚英
焦春海
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Hubei Zhaozhi Modern Agricultural Technology Co ltd
Institute of Economic Crop of Hubei Academy of Agricultural Science
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Hubei Zhaozhi Modern Agricultural Technology Co ltd
Institute of Economic Crop of Hubei Academy of Agricultural Science
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Priority to CN202011222403.2A priority Critical patent/CN112243858A/en
Publication of CN112243858A publication Critical patent/CN112243858A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention relates to the technical field of plant breeding methods, and particularly discloses a hot pepper self-pollination method and application thereof.

Description

Selfing pollination method for hot peppers and application of selfing pollination method
Technical Field
The invention relates to the technical field of plant breeding methods, in particular to a hot pepper selfing pollination method and application thereof.
Background
Capsicum, also called ox horn pepper, long pepper, vegetable pepper, and lantern pepper, is a perennial or limited herbaceous plant, and capsicum belongs to a plant with cross pollination, and the purification of parent material usually requires artificial pollination. Therefore, the pepper pollination method has high and low working efficiency, and has important significance for producing pepper serving as important vegetables and seasonings so as to meet the daily life requirements of people.
At present, the content of pepper pollination methods is mostly artificial pollination methods, flowers to be pollinated need to be marked before artificial pollination, and a listing method is often adopted, namely, a thin line with a small plastic plate is sleeved in a flower stem. However, the method in the above technical solution has the following disadvantages: firstly, in the process of artificial selfing pollination, the stigma is very easy to be broken when in bud peeling and pollination, so that the pollination fails; secondly, after artificial selfing pollination, the stigmas are still pollinated for the second time after the insects are infected with exogenous pollen, so that pollen pollution is caused; thirdly, the plastic cards have certain weight, so that the pedicel can be tightened, the barrier of nutrient substance transmission of the pedicel is caused, and the normal growth of the fruit is influenced; fourthly, when the environmental wind power is large, the small plastic cards can be pulled down together with the flower stalks under the action of the wind power, so that the pollination fails; and fifthly, long time is consumed for artificial pollination, artificial flower soaking and pollination in the process of positioning selfing flowers by hanging the cards and hanging the plastic cards. Therefore, the method for rapid selfing and pollination of the peppers is designed, and theoretical and technical support can be provided for industrial cultivation of the peppers.
Disclosure of Invention
The embodiment of the invention aims to provide a self-pollination method for hot peppers, which aims to solve the problems that most of the existing hot pepper pollination methods proposed in the background art adopt artificial pollination methods, pollen pollution is easily caused, pollination is easily failed, normal growth of fruits is influenced, and time consumption is too long.
In order to achieve the above purpose, the embodiments of the present invention provide the following technical solutions:
a selfing pollination method for hot peppers comprises the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a flower bud from a marked selfing single plant of the pepper for flower covering, wherein the flower covering is to cover the whole part of the flower bud and the flower stalk of the part corresponding to the flower bud by a sleeve, and then knotting and sealing the opening end of the sleeve.
The embodiment of the invention also aims to provide application of the self-pollination method for the hot pepper in hot pepper cultivation.
As a still further scheme of the invention: in the application of the self-pollination method of the pepper in pepper cultivation, the pepper can be generally selected from the following three types, namely the first type is lantern pepper, the second type is pod pepper and the third type is cluster pepper. Specifically, the species of pepper to be cultivated can be selected according to the need, and is not limited herein.
Compared with the prior art, the invention has the beneficial effects that:
the embodiment of the invention provides a hot pepper selfing pollination method, which is characterized in that the sleeve is adopted for selfing pollination of hot peppers, the working procedure is simple, the isolation effect is good, the labor consumption is low, the cost is low, only the sleeve needs to cover a flower bud, and knotting and sealing are carried out, so that the selfing pollination efficiency of the flower bud is improved, and the selfing pollination quality is ensured.
The material cost of the embodiment of the invention is low, the material used by the sleeve is common copy paper, the paper area used by a single sleeve is small, and the cost is less than 0.003 yuan; the colored metal wire only consumes about 4cm in length each time, is light in weight, not easy to fade, reusable and low in price.
And thirdly, the self-pollination of the material is ensured, the interference of entomophily and anemophily is isolated by the self-pollination in the sleeve, and the self-pollination in a single flower is completely realized.
The embodiment of the invention is easy to distinguish selfing fruits or non-selfing fruits, and the color binding wires formed by the metal wires have bright colors and are easy to discover; and is fixed on the fruit stalks and can not fall off with the wind; the method has the advantages of real-time fruit harvesting, easiness and simplicity, solves the problems that most of the existing hot pepper pollination methods adopt artificial pollination methods, pollen pollution is easily caused, pollination is easy to fail, normal growth of fruits is influenced, and time consumption is too long, and has wide market prospect.
Drawings
Fig. 1 is a schematic diagram of a self-pollination method for capsicum, provided by an embodiment, after completion.
In the figure: 1-a sleeve; 2-flower bud; 3-pedicel; 4-wire.
Detailed Description
The invention is described in further detail below with reference to the figures and specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Referring to fig. 1, in an embodiment of the present invention, a self-pollination method for hot peppers is provided, and specifically, a rapid self-pollination method for hot peppers includes the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a flower bud 2 to be opened from a marked selfed pepper single plant for flower covering, wherein the flower covering is to cover the whole part of the flower bud 2 and a flower stalk 3 of a part (generally a half) corresponding to the flower bud 2 by a sleeve 1, and then knotting and sealing the opening end of the sleeve 1.
It should be noted that the selection of the selfing individual plants of the peppers to be pollinated is to select excellent selfing individual plants of the peppers: in the flowering period, selecting the required excellent selfing individual plants of the hot pepper according to the breeding target, and hanging the plastic plate with bright color at the main branch for marking.
In another preferred embodiment of the present invention, the sleeve 1 has a cylindrical tubular structure with one end closed and the other end provided with an opening, and the sleeve 1 is manufactured in advance before use. Of course, the structure may have other shapes, such as a hollow cube or a hollow truncated cone, and the structure is selected according to the requirement, and the structure is not limited herein. Preferably, the sleeve 1 is a cylindrical structure with one end closed and the other end provided with an opening.
As another preferred embodiment of the present invention, the material of the sleeve 1 may be paper, plastic, metal, etc., and is specifically selected according to the requirement, which is not limited herein.
Preferably, the sleeve 1 is a paper sleeve, and specifically, the sleeve 1 is manufactured by cutting a copy paper of a certain specification into square paper, rolling the paper into a cylindrical tubular structure, and sealing one end of the cylindrical tubular structure to form a cylindrical tubular structure with one end sealed and the other end provided with an opening.
As another preferred embodiment of the invention, the diameter of the sleeve 1 is 4-20 mm.
As another preferred embodiment of the invention, the diameter of the sleeve 1 is 8-10 mm.
Preferably, the sleeve 1 is made of square copy paper with the side length of 5cm, and is curled by a common ball pen to form a cylindrical tubular structure with the diameter of 8-10mm, wherein one end of the cylindrical tubular structure is closed, and the other end of the cylindrical tubular structure is opened.
In another preferred embodiment of the present invention, the flower set is made by covering the whole flower bud 2 and half of the flower stalk 3 corresponding to the flower bud 2 with the sleeve 1, and then tying and sealing with the metal wire 4 in a sunny day or a sunny day.
As another preferred embodiment of the present invention, the knotted seal is made by using the metal wire 4, specifically, the open end of the sleeve 1 is tightened and knotted with the pedicel 3 through the metal wire 4 to complete the seal (the metal wire 4 is wound around the open end of the sleeve 1 to perform knotted seal).
As another preferred embodiment of the present invention, the color of the metal wire 4 can be selected according to the needs, and is not limited herein, for example, it can be colored, or black or white, or transparent, and preferably, the knotted seal is the colored metal wire 4.
As another preferred embodiment of the present invention, the number of flower buds selected per selfed individual capsicum is 1 to 30.
As another preferred embodiment of the invention, the sleeve 1 and the pedicel 3 at the knotted knot retain 1-3mm of pores, namely the distance between the sleeve 1 and the pedicel 3 is 1-3mm after the opening end of the sleeve 1 is sealed.
As another preferred embodiment of the present invention, the sleeve 1 and the pedicel 3 at the knotted knot remain 1-2mm in porosity.
As another preferred embodiment of the present invention, the length of the wire 4 is 4-6cm, and specifically, the colored wire 4 having a length of about 4-6cm is cut off for use, and the colored wire 4 is wound around the outside of the open end of the sleeve 1.
In the embodiment of the invention, the sleeve 1 is adopted to perform self-pollination on the peppers, the process is simple, but the sleeve 1 has a good isolation effect on the flower buds 2, so that the interference of entomophily and anemophily can be effectively isolated, and the self-pollination in a single flower is completely realized. Therefore, compare in prior art, this scheme can effectively practice thrift operating time, improves the selfing pollination efficiency of bud 2, guarantees the selfing pollination quality simultaneously.
As another preferred embodiment of the invention, in the step of selecting the flower buds 2 to be opened for flower set, the number of the flower buds 2 to be opened selected by each self-bred pepper plant is 4-20, specifically, the number of the flower buds 2 to be opened is 10-20 (sweet pepper is kept at 4-5) at different branches on the marked self-bred pepper plant, and the self-crossing effect of the sleeve 1 on the 2 nd layer to the 4 th layer of the pepper of the self-bred pepper plant is optimal.
As another preferred embodiment of the present invention, in the self-pollination method for pepper, further comprising the step of harvesting a self-pollinated fruit, wherein the harvested self-pollinated fruit is a pepper fruit with a colored wire 4 mark provided on the stem thereof at the physiological mature stage of pepper; wherein, after selfing and pollination of the pepper, conventional management is carried out according to the prior art, and pepper fruits are finally obtained.
The embodiment of the invention also aims to provide application of the self-pollination method for the hot pepper in hot pepper cultivation.
As another preferred embodiment of the present invention, in the application of the self-pollination method of pepper in pepper cultivation, the pepper can be generally selected from the following three types, the first type is Lantern pepper, the second type is pod pepper, the pepper belongs to a variety, the flavor is similar to green pepper, and the third type is clustered pepper. Specifically, the kind of pepper to be cultivated can be selected according to the need, and is not limited herein.
The technical effects of the selfing pollination method for hot pepper of the present invention will be further described below by referring to specific examples.
Example 1
A selfing pollination method for hot peppers comprises the following steps:
1) selecting excellent individual plants: in the flowering period, selecting the required excellent pepper selfing individual plant according to the breeding target, and hanging a plastic plate with bright color at the main branch for marking;
2) selecting a bud to be opened 2: selecting 10-20 buds to be opened at different branches of the marked selfed pepper individual plant (the number of sweet peppers is kept between 4 and 5);
3) manufacturing a sleeve 1: cutting a piece of copy paper with a certain specification into square paper with the size of 5cm multiplied by 5cm, rolling the square paper into a cylindrical structure with the diameter of 8-10mm by using a common ball pen, and sealing one end of the cylindrical structure;
4) flower sleeving: selecting the sunny day or the sunny day, and covering the whole flower bud 2 and half of the flower stalks 3 with the sleeve 1; the sleeve 1 is used on the 2 nd layer to the 4 th layer of the selfing single plant of the pepper to achieve the best selfing effect;
5) and (3) knotting and sealing: cutting off a colorful metal wire 4 with the length of about 4-6cm, tying the open end of the sleeve 1 and the pedicel 3 through the metal wire 4, and keeping a hole with the diameter of 1-2mm between the sleeve 1 and the pedicel 3 at the knot;
6) collecting selfed fruits: 6-8 fruits with color binding wire marks on fruit stalks are harvested in the physiological maturation period, and the success rate reaches 60%.
Example 2
A hot pepper selfing pollination method, in particular to a hot pepper rapid selfing pollination method, which comprises the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a bud 2 to be opened from a marked selfed pepper single plant for flower covering, wherein the flower covering is specifically to cover the sleeve 1 on the whole part of the bud 2 and a peduncle 3 of a part (generally a half) corresponding to the bud 2, and then knotting and sealing the opening end of the sleeve 1; the sleeve 1 is made of square copy paper with the side length of 5cm, a common ball pen is used for curling to form a cylindrical structure with the diameter of 8mm, one end of the cylindrical structure is closed, and the other end of the cylindrical structure is opened.
Example 3
A hot pepper selfing pollination method, in particular to a hot pepper rapid selfing pollination method, which comprises the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a bud 2 to be opened from a marked selfed pepper single plant for flower covering, wherein the flower covering is specifically to cover the sleeve 1 on the whole part of the bud 2 and a peduncle 3 of a part (generally a half) corresponding to the bud 2, and then knotting and sealing the opening end of the sleeve 1; the sleeve 1 is made of square copy paper with the side length of 5cm, a common ball pen is used for curling to form a cylindrical structure with the diameter of 10mm, one end of the cylindrical structure is closed, and the other end of the cylindrical structure is opened.
Example 4
The same as example 3 was repeated except that the sleeve 1 was a cylindrical tubular structure having a diameter of 9mm as compared with example 3.
Example 5
A hot pepper selfing pollination method, in particular to a hot pepper rapid selfing pollination method, which comprises the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a flower bud 2 to be opened from a marked selfed pepper single plant for flower covering, wherein the flower covering is specifically to select sunny days or sunny days, covering the whole part of the flower bud 2 and the flower stalks 3 of the corresponding part (generally half) of the flower bud 2 with a sleeve 1, and then knotting and sealing the opening end of the sleeve 1; the sleeve 1 is made of square copy paper with the side length of 5cm, a common ball pen is used for curling to form a cylindrical structure with the diameter of 9mm, one end of the cylindrical structure is closed, and the other end of the cylindrical structure is opened; the selection of the buds 2 to be opened is that the number of the buds 2 to be opened is 10 at different branches of the marked selfed single plant of the pepper; the knotting seal is to cut off a colorful metal wire 4 with the length of about 4cm, tighten the opening end of the sleeve 1 and to be knotted and sealed with the pedicel 3 through the metal wire 4, and the sleeve 1 and the pedicel 3 at the knotting position are reserved with a hole with the diameter of 1 mm.
Example 6
A hot pepper selfing pollination method, in particular to a hot pepper rapid selfing pollination method, which comprises the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a flower bud 2 to be opened from a marked selfed pepper single plant for flower covering, wherein the flower covering is specifically to select sunny days or sunny days, covering the whole part of the flower bud 2 and the flower stalks 3 of the corresponding part (generally half) of the flower bud 2 with a sleeve 1, and then knotting and sealing the opening end of the sleeve 1; the sleeve 1 is made of square copy paper with the side length of 5cm, a common ball pen is used for curling to form a cylindrical structure with the diameter of 9mm, one end of the cylindrical structure is closed, and the other end of the cylindrical structure is opened; the selection of the buds 2 to be opened is that the number of the buds 2 to be opened is 20 at different branches of the marked selfed single plant of the capsicum; the knotting seal is to cut off a colorful metal wire 4 with the length of about 6cm, tighten the opening end of the sleeve 1 and to be knotted and sealed with the pedicel 3 through the metal wire 4, and the sleeve 1 and the pedicel 3 at the knotting position are reserved with a hole of 2 mm.
Example 7
A hot pepper selfing pollination method, in particular to a hot pepper rapid selfing pollination method, which comprises the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a flower bud 2 to be opened from a marked selfed pepper single plant for flower covering, wherein the flower covering is specifically to select sunny days or sunny days, covering the whole part of the flower bud 2 and the flower stalks 3 of the corresponding part (generally half) of the flower bud 2 with a sleeve 1, and then knotting and sealing the opening end of the sleeve 1; the sleeve 1 is made of square copy paper with the side length of 5cm, a common ball pen is used for curling to form a cylindrical structure with the diameter of 9mm, one end of the cylindrical structure is closed, and the other end of the cylindrical structure is opened; the selection of the buds 2 to be opened is that the number of the buds 2 to be opened is 15 at different branches of the marked selfed single plant of the capsicum; the knotting seal is to cut off a colorful metal wire 4 with the length of about 5cm, tighten the opening end of the sleeve 1 and to be knotted and sealed with the pedicel 3 through the metal wire 4, and the sleeve 1 and the pedicel 3 at the knotting position are reserved with a hole with the diameter of 1.5 mm.
Example 8
The same as example 7, except that the number of the flower buds 2 to be opened was 4, as compared with example 7, was selected.
Example 9
The same as example 7, except that the number of the flower buds 2 to be opened was 5, as compared with example 7, was selected.
Example 10
The same as example 7, except that the number of the flower buds 2 to be opened was 8, as compared with example 7, was selected.
Example 11
The same as example 7, except that the number of flower buds 2 to be opened was 18, as compared with example 7, was selected.
Example 12
The same as example 7 except that the length of the wire 4 was 4.2cm as compared with example 7.
Example 13
The same as example 7 except that the length of the wire 4 was 4.5cm as compared with example 7.
Example 14
The same as example 7 except that the length of the wire 4 was 5.2cm as compared with example 7.
Example 15
The same as example 7 except that the length of the wire 4 was 5.7cm as compared with example 7.
Example 16
Compared with example 7, the method is the same as example 7 except that the sleeve 1 at the knot part and the pedicel 3 keep a pore with the diameter of 1.8 mm.
The invention has the beneficial effects that the embodiment of the invention provides a hot pepper selfing pollination method, which comprises the steps of selecting excellent single plants, selecting buds to be opened, manufacturing a sleeve, sleeving flowers, knotting and sealing metal wires, collecting selfed fruits and the like, can timely and accurately finish a large amount of work, is a simple, quick and stable hot pepper selfing pollination method, and particularly adopts the sleeve 1 to perform hot pepper selfing pollination, so that the working procedure is simple, the sleeve 1 has a good isolation effect on the buds 2, the interference of entomophilous agents and anemophilous agents can be effectively isolated, the self-pollination in a single flower can be completely realized, compared with the prior art, the working time can be effectively saved, the selfing pollination efficiency of the flower buds 2 can be improved, the selfing pollination quality can be ensured, the anti-interference performance is good, the breeding efficiency is high, and the wide market prospect is achieved.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the scope of the invention.

Claims (10)

1. A self-pollination method of hot pepper is characterized by comprising the following steps:
1) selecting selfing single plants of the hot peppers to be pollinated, and carrying out listing marking;
2) selecting a flower bud from a marked selfing single plant of the pepper for flower covering, wherein the flower covering is to cover the whole part of the flower bud and the flower stalk of the part corresponding to the flower bud by a sleeve, and then knotting and sealing the opening end of the sleeve.
2. The method for self-pollination of pepper as claimed in claim 1, wherein in the method for self-pollination of pepper, the sleeve is closed at one end and provided with an opening at the other end.
3. A self-pollination method for pepper as claimed in claim 2, wherein in the self-pollination method for pepper, the diameter of the sleeve is 4-20 mm.
4. A self-pollination method for pepper as claimed in claim 3, wherein in the self-pollination method for pepper, the diameter of the sleeve is 8-10 mm.
5. A self-pollination method for pepper as claimed in claim 1, wherein in the self-pollination method for pepper, the sealing by knotting is performed by tightening the open end of the sleeve and knotting the pedicel.
6. The self-pollination method of pepper as claimed in claim 5, wherein in the self-pollination method of pepper, the sleeve and the pedicel at the knotted knot remain 1-3mm of pores.
7. The method for self-pollination of pepper as claimed in claim 1, wherein in the method for self-pollination of pepper, the number of flower buds selected per selfed individual plant of pepper is 1-30.
8. The method for self-pollination of pepper as claimed in claim 1, wherein in the method for self-pollination of pepper, the number of flower buds selected per selfed individual plant of pepper is 4-20.
9. A method for self-pollination of pepper as claimed in any one of claims 1-8, wherein said method for self-pollination of pepper further comprises the step of harvesting the self-pollinated fruit.
10. Use of a method of selfing pepper as claimed in any one of the claims 1-9 for pepper cultivation.
CN202011222403.2A 2020-11-05 2020-11-05 Selfing pollination method for hot peppers and application of selfing pollination method Pending CN112243858A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112806256A (en) * 2021-02-19 2021-05-18 杭州市农业科学研究院 Simple method for selfing and pollinating eggplants
CN114793889A (en) * 2022-06-21 2022-07-29 包头市农牧科学技术研究所 Selfing pollination method for hot peppers and application of selfing pollination method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1843089A (en) * 2006-05-15 2006-10-11 西北农林科技大学 Artificial bagging selfing pollination method for corn
CN104126389A (en) * 2014-07-22 2014-11-05 胡能兵 Method for selecting early-maturing yellow skin pepper germplasm
CN105475122A (en) * 2016-02-01 2016-04-13 何其家 Rapid selfing isolation method for purple bell peppers in greenhouse

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1843089A (en) * 2006-05-15 2006-10-11 西北农林科技大学 Artificial bagging selfing pollination method for corn
CN104126389A (en) * 2014-07-22 2014-11-05 胡能兵 Method for selecting early-maturing yellow skin pepper germplasm
CN105475122A (en) * 2016-02-01 2016-04-13 何其家 Rapid selfing isolation method for purple bell peppers in greenhouse

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112806256A (en) * 2021-02-19 2021-05-18 杭州市农业科学研究院 Simple method for selfing and pollinating eggplants
CN114793889A (en) * 2022-06-21 2022-07-29 包头市农牧科学技术研究所 Selfing pollination method for hot peppers and application of selfing pollination method

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Application publication date: 20210122