CN108055993B - Preparation method of nutrient solution for germination of bletilla striata in seedling stage of Dabie mountain - Google Patents
Preparation method of nutrient solution for germination of bletilla striata in seedling stage of Dabie mountain Download PDFInfo
- Publication number
- CN108055993B CN108055993B CN201711312285.2A CN201711312285A CN108055993B CN 108055993 B CN108055993 B CN 108055993B CN 201711312285 A CN201711312285 A CN 201711312285A CN 108055993 B CN108055993 B CN 108055993B
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- China
- Prior art keywords
- nutrient solution
- germination
- bletilla striata
- solution formula
- seed
- Prior art date
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 43
- 241001313857 Bletilla striata Species 0.000 title claims abstract description 29
- 230000035784 germination Effects 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000010521 absorption reaction Methods 0.000 claims abstract description 4
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 4
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 6
- 239000003242 anti bacterial agent Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 5
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 5
- 239000004571 lime Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000009331 sowing Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 4
- 150000004985 diamines Chemical class 0.000 claims description 3
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 3
- WTHDKMILWLGDKL-UHFFFAOYSA-N urea;hydrate Chemical compound O.NC(N)=O WTHDKMILWLGDKL-UHFFFAOYSA-N 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 9
- 230000008961 swelling Effects 0.000 abstract description 2
- 230000002786 root growth Effects 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 235000006180 nutrition needs Nutrition 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for preparing nutrient solution for seedling-stage germination of bletilla striata, belonging to the field of traditional Chinese pharmacology, wherein the method for preparing the nutrient solution for seedling-stage germination of the bletilla striata breaks through only one layer of cell seed coat in embryo water absorption and swelling to generate false roots; protocorm and leaf primordium formation phase; the protocorm continues to expand, and three nutrient solution formulas are respectively prepared during three periods of the true root growth period. The bletilla striata seeds treated by the nutrient solution have high germination rate and short germination time, wherein the germination rate is 95 percent, and the germination time is 15 days.
Description
Technical Field
The invention relates to the field of traditional Chinese pharmacology, in particular to a preparation method of a nutrient solution for seedling-stage germination of bletilla striata in the Dabie mountain.
Background
Bletilla striata is a perennial herb bulbous plant, and the plant height is 18-60 cm. Mainly distributed in china, japan and northern burma. The main flowering period is in spring, but depending on the climate in each area, flowers may appear from late winter to early summer. Bletilla striata has wide medicinal value and garden value. It is mainly used for astringing to stop bleeding, eliminating swelling and promoting granulation. The flower has purple red, white, blue, yellow and pink colors, can be viewed in a potted plant room, and can also be embellished on a shaded flower table, a flower environment or a corner of a courtyard. Many wild bletilla striata grow in the Dabie mountain region, and the wild bletilla striata is gradually cultivated artificially. However, some nutrient solution needs to be filled to help the growth of the bletilla striata during seedling germination, and the existing bletilla striata nutrient solution is not suitable for cultivating the bletilla striata in the Dabieshan mountain area. Bletilla striata in Dabieshan area has fine seeds without endosperm, nutrition needs to be added in the seed germination process, so that the contradiction between matrix configuration and nutritional ingredients is caused, and meanwhile, mixed bacteria needs to be controlled due to the addition of the nutritional ingredients, so that the problem that the conventional nutrient solution cannot be well solved.
Disclosure of Invention
The invention aims to solve the problem that the contradiction between matrix configuration and nutrient components caused by the addition of nutrition in the bletilla striata seed germination process and the problem that mixed bacteria need to be controlled due to the addition of the nutrient components are solved by providing a method for preparing nutrient solution for the seedling-stage germination of the wild bletilla striata in the Dabie mountain.
In order to achieve the purpose, the invention adopts the technical scheme that:
the preparation method of the nutrient solution for the seedling-stage germination of the bletilla striata in the Dabie mountain comprises the following steps:
(1) sowing and seed treatment: seed dressing agent and bletilla striata seeds are needed for seed dressing in seed sowing, a 40-mesh sieve is used for uniformly dressing the seeds, and naphthylacetic acid is used for treating the seeds, wherein the using concentration of the naphthylacetic acid is 2 mg/L;
(2) embryo water absorption and expansion break through only one layer of cell seed coat, rhizoid appears, the service time of the nutrient solution formula 1 is 15 days, and the nutrient solution formula 1: 1/4MS and 6-BA (1mg/L), with ppm antibacterial agent, at a concentration of 2 mg/L;
(3) protocorm and leaf primordium formation period, the using time of the nutrient solution formula 2 is 30 days, and the nutrient solution formula 2: 1/2MS and 6-BA (1mg/L), with ppm antibacterial agent, at a concentration of 2 mg/L;
(4) the protocorm continues to expand, the growth period of the true root is long, the service time of the nutrient solution formula 3 is 105 days, and the nutrient solution formula 3 is as follows: 1/2MS and 6-BA (1mg/L), and adding phosphoric diamine water and urea water in the middle at a volume ratio of 30: 1.
Preferably, the raw material of the seed dressing agent in the step (1) is lime powder and activated carbon, and the volume ratio of the lime powder to the activated carbon is 50: 1.
Has the advantages that: the preparation method of the nutrient solution for seedling-stage germination of the bletilla striata in the Dabie mountain wild species solves the problems that nutrition needs to be added in the germination process of the bletilla striata seeds, so that the contradiction between the configuration of a matrix and the nutrient components is caused, and meanwhile, the mixed bacteria needs to be controlled due to the addition of the nutrient components. The bletilla striata seeds treated by the nutrient solution have high germination rate and short germination time, wherein the germination rate is 95 percent, and the germination time is 15 days.
Detailed Description
The following describes in detail a preferred embodiment of the method for preparing the nutrient solution for germination of bletilla striata in seedling stage.
The preparation method of the nutrient solution for the seedling-stage germination of the bletilla striata in the Dabie mountain comprises the following steps:
(1) sowing and seed treatment: seed dressing agent and bletilla striata seeds are needed for seed dressing, a 40-mesh sieve is used for uniformly dressing the seeds, naphthylacetic acid is used for seed treatment, the using concentration of the naphthylacetic acid is 2mg/L, the raw materials of the seed dressing agent are lime powder and activated carbon, and the volume ratio of the raw materials to the activated carbon is 50: 1;
(2) embryo water absorption and expansion break through only one layer of cell seed coat, rhizoid appears, the service time of the nutrient solution formula 1 is 15 days, and the nutrient solution formula 1: 1/4MS and 6-BA (1mg/L), with ppm antibacterial agent, at a concentration of 2 mg/L;
(3) protocorm and leaf primordium formation period, the using time of the nutrient solution formula 2 is 30 days, and the nutrient solution formula 2: 1/2MS and 6-BA (1mg/L), with ppm antibacterial agent, at a concentration of 2 mg/L;
(4) the protocorm continues to expand, the growth period of the true root is long, the service time of the nutrient solution formula 3 is 105 days, and the nutrient solution formula 3 is as follows: 1/2MS and 6-BA (1mg/L), and adding phosphoric diamine water and urea water in the middle at a volume ratio of 30: 1.
The preparation method of the nutrient solution for seedling-stage germination of the bletilla striata in the Dabie mountain wild species solves the problems that nutrition needs to be added in the germination process of the bletilla striata seeds, so that the contradiction between the configuration of a matrix and the nutrient components is caused, and meanwhile, the mixed bacteria needs to be controlled due to the addition of the nutrient components. The bletilla striata seeds treated by the nutrient solution have high germination rate and short germination time, wherein the germination rate is 95 percent, and the germination time is 15 days.
The above description is only an example of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and embodiments, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (1)
1. A preparation method of a nutrient solution for germination of bletilla striata in seedling stage is characterized by comprising the following steps:
(1) sowing and seed treatment: seed dressing agent and bletilla striata seeds are needed for seed dressing during seed sowing, the seeds are evenly screened by a 40-mesh sieve, and naphthylacetic acid is used for seed treatment, wherein the use concentration of the naphthylacetic acid is 2 mg/L; the raw materials of the seed dressing agent are lime powder and active carbon, and the volume ratio of the lime powder to the active carbon is 50: 1;
(2) embryo water absorption and expansion break through only one layer of cell seed coat, rhizoid appears, the service time of the nutrient solution formula 1 is 15 days, and the nutrient solution formula 1: 1/4MS and 6-BA, using PPM antibacterial agent, the using concentration is 2 mg/L;
(3) protocorm and leaf primordium formation period, the using time of the nutrient solution formula 2 is 30 days, and the nutrient solution formula 2: 1/2MS and 6-BA, using PPM antibacterial agent, the using concentration is 2 mg/L;
(4) the protocorm continues to expand, the growth period of the true root is long, the service time of the nutrient solution formula 3 is 105 days, and the nutrient solution formula 3 is as follows: 1/2MS and 6-BA, adding phosphoric acid diamine water and urea water in the middle, wherein the volume ratio is 30: 1;
the concentration of 6-BA used in the above step was 1 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711312285.2A CN108055993B (en) | 2017-12-01 | 2017-12-01 | Preparation method of nutrient solution for germination of bletilla striata in seedling stage of Dabie mountain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711312285.2A CN108055993B (en) | 2017-12-01 | 2017-12-01 | Preparation method of nutrient solution for germination of bletilla striata in seedling stage of Dabie mountain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108055993A CN108055993A (en) | 2018-05-22 |
| CN108055993B true CN108055993B (en) | 2021-09-28 |
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| CN201711312285.2A Expired - Fee Related CN108055993B (en) | 2017-12-01 | 2017-12-01 | Preparation method of nutrient solution for germination of bletilla striata in seedling stage of Dabie mountain |
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Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246695B (en) * | 2011-05-10 | 2012-09-26 | 云南植物药业有限公司 | Method for preparing common bletilla pseudobulb in culture vessel and special culture media thereof |
| CN103828715B (en) * | 2013-11-29 | 2017-01-18 | 安徽中升生物科技有限公司 | Bletilla striata tissue culture breeding method |
| CN104272952A (en) * | 2014-10-16 | 2015-01-14 | 合肥长润农业科技有限公司 | Planting method of pollution-free bitter melons |
| CN105340740A (en) * | 2015-11-10 | 2016-02-24 | 云南师范大学 | Liquid high-efficiency germination induction solution and rapid propagation method for bletilla striata seeds |
| CN106233875A (en) * | 2016-08-26 | 2016-12-21 | 滁州菲扬农业科技有限公司 | The method improving Semen Coriandri germination percentage |
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| CN108055993A (en) | 2018-05-22 |
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Granted publication date: 20210928 |