CN108014037B

CN108014037B - Nourishing moisturizer and preparation method thereof

Info

Publication number: CN108014037B
Application number: CN201610962131.7A
Authority: CN
Inventors: 李红
Original assignee: Wuxi Farurya Biological Cell Science Co ltd
Current assignee: Wuxi Farurya Biological Cell Science Co ltd
Priority date: 2016-11-04
Filing date: 2016-11-04
Publication date: 2020-10-09
Anticipated expiration: 2036-11-04
Also published as: CN108014037A

Abstract

The invention belongs to the field of cosmetics, and relates to nourishing moisturizer and a preparation method thereof. Specifically, the nourishing and moisturizing cream disclosed by the invention comprises the following components in parts by weight: 2-5 parts of bighead atractylodes rhizome extract, 2-5 parts of white peony root extract, 4-8 parts of glossy privet fruit extract, 1-3 parts of edetate disodium, 4-8 parts of vitamin C, 5-10 parts of Tween-80, 1-3 parts of beta-sitosterol, 4-8 parts of squalene, 4-8 parts of vitamin E, 2-5 parts of glycerol and 100 parts of deionized water. The nourishing moisturizer mainly takes plant extracts as active ingredients, the bighead atractylodes rhizome extract has the effects of improving skin and whitening, the white paeony root extract has the effects of resisting inflammation and regulating immunity, and the glossy privet fruit extract has the effects of delaying senility, resisting bacteria and preserving. The nourishing moisturizer can realize long-term moisturizing and moisture-keeping effects, does not use artificially synthesized organic preservatives, and has natural and non-irritating raw materials.

Description

Nourishing moisturizer and preparation method thereof

Technical Field

The invention belongs to the field of cosmetics, and relates to nourishing moisturizer and a preparation method thereof.

Background

The skin cream is a semisolid cosmetic with high viscosity and difficult flowing, and is suitable for dry skin. After cleaning, the skin care product is immediately applied to the skin to play the roles of moisturizing, moistening, promoting the physiological function of the skin and the like.

Organic substances such as phenoxyethanol, butyl p-hydroxybenzoate, potassium sorbate, sodium salicylate and the like are generally added as preservatives to the existing skin cream products on the market. Although the substances can provide a preservative effect for the moisturizer and prolong the shelf life of the product, the substances have certain toxicity, and can cause irritation and damage to human skin to a certain extent after long-term use. In addition, the existing moisturizer products have unsatisfactory moisturizing and nourishing effects, and further improvement is urgently needed.

Disclosure of Invention

In order to overcome the technical problems, the invention aims to provide a nourishing and moisturizing cream which does not contain organic preservatives and has a moisturizing effect and a preparation method thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

the nourishing and moisturizing cream comprises the following components in parts by weight: 2-5 parts of bighead atractylodes rhizome extract, 2-5 parts of white peony root extract, 4-8 parts of glossy privet fruit extract, 1-3 parts of edetate disodium, 4-8 parts of vitamin C, 5-10 parts of Tween-80, 1-3 parts of beta-sitosterol, 4-8 parts of squalene, 4-8 parts of vitamin E, 2-5 parts of glycerol and 100 parts of deionized water.

Preferably, the nourishing and moisturizing cream comprises the following components in parts by weight: 2.5-4 parts of bighead atractylodes rhizome extract, 2.5-4 parts of white peony root extract, 5-7 parts of glossy privet fruit extract, 1.5-2.5 parts of disodium edetate, 5-7 parts of vitamin C, 6-9 parts of Tween-80, 1.5-2.5 parts of beta-sitosterol, 5-7 parts of squalene, 5-7 parts of vitamin E, 2.5-4 parts of glycerol and 100 parts of deionized water.

More preferably, the nourishing and moisturizing cream comprises the following components in parts by weight: 3 parts of bighead atractylodes rhizome extract, 3 parts of white paeony root extract, 6 parts of glossy privet fruit extract, 2 parts of edetate disodium, 6 parts of vitamin C, 8 parts of tween-80, 2 parts of beta-sitosterol, 6 parts of squalene, 6 parts of vitamin E, 3 parts of glycerol and 100 parts of deionized water.

Atractylodis rhizoma (rhizoma Atractylodis Macrocephalae) is the rhizome of Atractylodes macrocephala Koidz (Atractylodes macrocephala Koidz) of Atractylodes of Compositae, and is distributed in Zhejiang, Hubei, Hunan, Jiangxi, Fujian, Anhui province. Collected in more than Nongri, has warm nature, bitter and sweet taste, enters spleen and stomach meridians, and is used for tonifying qi and spleen, eliminating dampness and diuresis, stopping sweating and preventing miscarriage. The Atractylodis rhizoma contains various volatile oil components (such as atractylenolide I and atractylone), has effects of invigorating qi, replenishing blood, whitening skin, moistening skin, and delaying aging, and is suitable for pachylosis, sallow complexion, chloasma, pigmentation, etc. caused by deficiency-cold of qi and blood.

In the nourishing moisturizer, the bighead atractylodes rhizome extract is prepared by the following method: cleaning and crushing the bighead atractylodes rhizome, and sieving the bighead atractylodes rhizome with a 100-300-mesh sieve to obtain bighead atractylodes rhizome powder; adding the white atractylodes rhizome powder into water according to the weight ratio of the white atractylodes rhizome powder to the water of 1: 8-12, soaking for 2-4 hours, and extracting volatile oil under the reflux condition until the amount of the volatile oil is not increased any more; and (3) standing for 0.5-2 hours, separating the volatile oil at the upper layer and the water at the lower layer, and drying the volatile oil by adopting a drying agent to obtain the bighead atractylodes rhizome extract.

Preferably, the extraction in the above method is performed by a volatile oil extraction tank.

Preferably, the drying agent in the above method is selected from any one of anhydrous sodium sulfate, anhydrous magnesium sulfate, and anhydrous calcium chloride.

Radix Paeoniae alba is root of Paeonia lactiflora Pall belonging to Paeonia of Ranunculaceae, and is mainly distributed in Zhejiang, Anhui, Sichuan province, etc. Collected in summer and autumn, slightly cold in nature, bitter and sour in taste, entering liver and spleen meridians, and indicated for nourishing blood, astringing yin, softening liver, alleviating pain and calming liver yang. Radix Paeoniae alba contains various monoterpene glycosides (such as paeoniflorin and albiflorin), has antiinflammatory, analgesic, anti-stress and immunoregulatory effects, and has concentration and function dependence characteristics.

In the nourishing moisturizer, the white paeony root extract is prepared by the following method: cleaning and crushing white paeony root, and sieving with a 100-300-mesh sieve to obtain white paeony root powder; adding the white paeony root powder into an ethanol aqueous solution with the ethanol volume fraction of 60-80% according to the weight ratio of the white paeony root powder to the ethanol aqueous solution of 1: 5-10, carrying out reflux extraction for 2-4 times, extracting for 60-120 minutes each time, centrifuging after the extraction is finished, separating supernate, concentrating until no alcohol smell exists, adding ethyl acetate, extracting for 3-5 times, combining ethyl acetate phases, and concentrating to obtain the white paeony root extract.

Fructus Ligustri Lucidi is the mature fruit of Ligustrum lucidum Ait (Ligustrum lucidum Ait) of Ligustrum of Oleaceae, and has wide distribution. Picked in winter when fruits are ripe, cool in nature, sweet and bitter in taste, enters liver and kidney meridians, and is used for tonifying liver and kidney, clearing heat and improving eyesight. Fructus Ligustri Lucidi contains various active ingredients mainly containing triterpenes (such as oleanolic acid and ursolic acid), and has effects of enhancing immunity and delaying aging, and the decoction has antiinflammatory, antibacterial, and chromosome injury resisting effects.

In the nourishing moisturizer, the glossy privet fruit extract is prepared by the following steps: cleaning and crushing glossy privet fruits, and sieving the glossy privet fruits by a sieve of 100-300 meshes to obtain glossy privet fruit powder; adding the glossy privet fruit powder into an ethanol aqueous solution with the volume fraction of ethanol being 60-80% according to the weight ratio of the glossy privet fruit powder to the ethanol aqueous solution being 1: 6-12, carrying out reflux extraction for 2-4 times, each time for 1-3 hours, centrifuging after the extraction is finished, separating supernate, concentrating until no alcohol smell exists, adding ethyl acetate, extracting for 3-5 times, combining ethyl acetate phases, and concentrating to obtain the glossy privet fruit extract.

A preparation method of the nourishing moisturizer comprises the following steps: firstly, mixing beta-sitosterol, squalene, vitamin E and an extract of bighead atractylodes rhizome according to the formula ratio, stirring and heating to 60-80 ℃ at the rotating speed of 100-500 rpm, and preserving heat for 10-30 minutes to obtain an oil phase component; mixing the white paeony root extract, the glossy privet fruit extract, the edetate disodium, the vitamin C, the Tween-80, the glycerol and the water according to the formula ratio, stirring and heating to 50-70 ℃ under the condition of the rotating speed of 100-500 rpm, and preserving the heat for 10-30 minutes to obtain a water phase component; and finally, adding the water phase component into the oil phase component, and emulsifying for 5-10 minutes at 50-60 ℃ under the condition of the rotating speed of 1000-3000 rpm to obtain the nourishing moisturizer.

In the above preparation method, the stirring is performed by a mechanical or magnetic stirring apparatus.

In the above preparation method, the emulsification is performed by a homogenizer.

The using method of the nourishing moisturizer comprises the following steps: firstly, cleaning the part (such as face, neck, hands and the like) needing to be applied with the skin cream, then applying a proper amount of the nourishing skin cream on the part, and gently beating and/or kneading the part to promote the absorption of the nourishing skin cream by the skin.

Compared with the prior art, the nourishing moisturizer mainly takes the plant extract as the active ingredient in the moisturizer, the white atractylodes rhizome extract has the effects of improving skin quality and whitening, the white paeony root extract has the effects of resisting inflammation and regulating immunity, and the glossy privet fruit extract has the effects of delaying senility, resisting bacteria and preventing corrosion. The nourishing moisturizer can realize long-term moisturizing and moisture-keeping effects, does not use artificially synthesized organic preservatives, and has natural and non-irritating raw materials.

Detailed Description

The technical solution of the present invention will be further explained with reference to specific embodiments. The following examples are provided only for explaining and illustrating the present invention and should not be construed as limiting the scope of the present invention. Unless otherwise indicated, the instruments, reagents, materials and the like used in the following examples are all available by conventional commercial means.

Example 1: preparing the extract of the white atractylodes rhizome.

Cleaning 1kg of Atractylodis rhizoma (purchased from Xishan area of Wuxi city, Chinese medicinal material wholesale market) with water, pulverizing, and sieving with 200 mesh sieve to obtain 0.7kg of Atractylodis rhizoma powder. Placing the rhizoma Atractylodis Macrocephalae powder in a volatile oil extraction tank, adding 7kg of water, soaking for 3 hours, and extracting volatile oil under reflux condition until the amount of volatile oil is not increased any more. Standing for 1 hr, separating volatile oil at upper layer and water at lower layer, and drying volatile oil with anhydrous sodium sulfate to obtain Atractylodis rhizoma extract.

Example 2: and (3) preparing a white paeony root extract.

1kg of white paeony root (purchased from the Wuxi mountain area Chinese medicinal material wholesale market in Wuxi city) is washed by water, crushed and sieved by a 200-mesh sieve to obtain 0.8kg of white paeony root powder. Adding the radix paeoniae alba powder into 8kg of ethanol aqueous solution (ethanol volume fraction is 70%), carrying out reflux extraction for 3 times, each time for 90 minutes, centrifuging by using a centrifugal machine after extraction is finished, separating supernate, placing the supernate in a rotary evaporator for concentration until no alcohol smell exists, adding equal volume of ethyl acetate for extraction for 4 times, combining ethyl acetate phases, placing the ethyl acetate phases in the rotary evaporator for concentration, and obtaining the radix paeoniae alba extract.

Example 3: and (3) preparing a glossy privet fruit extract.

1kg of glossy privet fruit (purchased from the Wuxi mountain area traditional Chinese medicinal material wholesale market in Wuxi city) is washed by water, crushed and sieved by a 200-mesh sieve to obtain 0.75kg of glossy privet fruit powder. Adding the glossy privet fruit powder into 6kg of ethanol aqueous solution (ethanol volume fraction is 70%), carrying out reflux extraction for 3 times, each time for 2 hours, centrifuging by using a centrifugal machine after extraction is finished, separating supernate, placing the supernate in a rotary evaporator for concentration until no alcohol smell exists, adding equal volume of ethyl acetate for extraction for 3 times, combining ethyl acetate phases, placing the ethyl acetate phases in the rotary evaporator for concentration, and obtaining the glossy privet fruit extract.

Example 4-example 8: preparing nourishing and moisturizing cream.

The nourishing moisturizer is prepared according to the formula in table 1, and the preparation process is as follows: firstly, mixing beta-sitosterol, squalene, vitamin E and an extract of bighead atractylodes rhizome, stirring and heating to 70 ℃ at the rotating speed of 300rpm by using a mechanical stirring device, and preserving heat for 20 minutes to obtain an oil phase component; mixing the white paeony root extract, the glossy privet fruit extract, the edetate disodium, the vitamin C, the tween-80, the glycerol and the water, stirring by adopting a mechanical stirring device at the rotating speed of 300rpm, heating to 60 ℃, and preserving the heat for 20 minutes to obtain a water phase component; and finally, adding the water phase component into the oil phase component, and emulsifying for 5 minutes at the rotating speed of 3000rpm by adopting a homogenizer at the temperature of 50 ℃ to obtain the nourishing moisturizer.

TABLE 1 formula and dosage of nourishing moisturizer

The nourishing skin cream is detected according to a test method in the light industry standard QB/T1857-2013 skin cream of the people's republic of China, and the result is shown in Table 2. As can be seen from Table 2, the nourishing moisturizer meets the requirements of the industrial standard and has development and application prospects.

Table 2. detection index results of nourishing moisturizer

Experimental example 1: and (3) stability investigation experiment of the nourishing moisturizer.

In order to evaluate the stability of the nourishing and moisturizing cream, atractylenolide I and atractylone in an atractylenolide extract, paeoniflorin and albiflorin in a white paeony root extract and oleanolic acid in a glossy privet fruit extract are respectively used as detection substances for investigation.

1ml of each of the skin creams of examples 4 to 8 was measured, diluted with deionized water and made to 20ml, and left to stand at 37 ℃. Samples were then taken at weeks 0, 1, 2, 3, 4, 5, 6 and 7 and the content was determined by GC-MS method or HPLC-MS method, the results of which are shown in Table 3.

TABLE 3 stability testing of active ingredients

As can be seen from Table 3, the content of the active substances in the nourishing and moisturizing cream is reduced a little along with the time, and after the nourishing and moisturizing cream is placed for 7 weeks, the final content of 5 detection substances can still be kept above 96%, which indicates that the active substances in the nourishing and moisturizing cream have relatively high stability and are beneficial to exerting a lasting effect.

Experimental example 2: skin irritation test of nourishing moisturizer.

Skin irritation tests were performed using the nourishing moisturizers prepared in examples 4-8. Selecting 30 rabbits with the weight of 2.0-2.5 kg as experimental animals, randomly dividing the experimental animals into 5 groups of 6 rabbits each with half of male and female animals. A region was shaved 24h before the test, about 10cm by 5cm on each side of the spine of the rabbit's back, with a pair of scissors and an electric razor, wherein the left side was coated with skin cream and the right side was a self-blank control. The application is carried out for 7 days 1 time per day, and the skin is observed for erythema and edema 5, 10, 30 and 60min after the skin cream is applied, and if there is obvious injury, the skin is taken for pathological histological examination.

The skin irritation response of the experimental animals was scored according to table 4, and after the average score was calculated, the irritation intensity was evaluated according to table 5.

TABLE 4 skin irritation response score

TABLE 5 evaluation of skin irritation Strength

Strength of	Score value
		Has no irritation	＜0.5
Mild irritation	＜2.0
		Moderate irritation	＜6.0
Strong irritation	＞6.0

TABLE 6 skin irritation test results

Test area	Example 4	Example 5	Example 6	Example 7	Example 8
						Skin area to be tested	0	0	0	0	0
Blank control zone	0	0	0	0	0

As can be seen from the results in Table 6, the skin-irritation test results of the nourishing moisturizer of the invention on rabbits are negative, and no obvious irritation effect is generated on the rabbit skin.

Experimental example 3: and (3) testing the moisturizing effect of the nourishing moisturizer.

20 women aged 20-40 years are selected as subjects, and are randomly divided into 5 groups of 4 persons. Use of

The moisture content of the skin in the forehead area of the subject was measured with a CM820 skin moisture tester (available from Courage-Khazaka, germany) at three time points before applying the moisturizer, 10 minutes after applying the moisturizer, and 1 hour after applying the moisturizer, and the results are shown in table 7, where the working environment temperature was 20 to 25 ℃, the relative humidity was 40 to 50%, and the moisture content unit was au (arbitrary unit).

TABLE 7 skin moisture content results before and after application of skin cream

Time of measurement	Example 4	Example 5	Example 6	Example 7	Example 8
						Before inunction (au)	32.5	32.3	33.5	33.4	32.2
After 10 minutes of inunction (au)	89.5	89.6	89.7	89.8	91.2
						After 1 hour of inunction (au)	76.8	75.7	76.3	76.5	78.5

From the results in table 7, it can be seen that the moisture content of the skin is significantly increased after the skin cream is applied, and the higher moisture content can be maintained within the time period of 1 hour, which indicates that the nourishing skin cream of the present invention has excellent moisturizing effect.

Experimental example 4: and (3) testing the moisturizing effect of the nourishing moisturizer.

20 women aged 20-40 years are selected as subjects, and are randomly divided into 5 groups of 4 persons, and the groups are respectively smeared with the nourishing moisturizers prepared in the examples 4-8. Before, half an hour after and 1 hour after the cream was applied, the moisturizing effect of the cream was scored according to the data in table 8, and the average score was calculated, and the results are shown in table 9.

TABLE 8 moisturizing effect scoring system

Moistening effect	Score value
		Slight discomfort	0
Has no obvious feeling	1
		Slight moistening sensation	2
Moistening sensation	3

TABLE 9 skin nourishing effect after skin cream application

Time of measurement	Example 4	Example 5	Example 6	Example 7	Example 8
						Before inunction (au)	1	1	1	1	1
After 0.5 hour of inunction (au)	2.5	2.75	2.5	2.75	3
						After 1 hour of inunction (au)	1.75	2.0	2.25	2.25	2.5

As can be seen from the results in table 9, the skin moisturizing effect is significantly improved after the skin cream is applied, and the skin moisturizing effect can be maintained within a time period of 1 hour, indicating that the nourishing skin cream of the present invention has an excellent moisturizing effect.

Claims

1. The nourishing and moisturizing cream is characterized by comprising the following components in parts by weight:

3 parts of bighead atractylodes rhizome extract, 3 parts of white paeony root extract, 6 parts of glossy privet fruit extract, 2 parts of edetate disodium, 6 parts of vitamin C, 8 parts of tween-80, 2 parts of beta-sitosterol, 6 parts of squalene, 6 parts of vitamin E, 3 parts of glycerol and 100 parts of deionized water.

2. The nourishing moisturizer of claim 1, wherein:

the bighead atractylodes rhizome extract is prepared by the following method: cleaning and crushing the bighead atractylodes rhizome, and sieving the bighead atractylodes rhizome with a 100-300-mesh sieve to obtain bighead atractylodes rhizome powder; adding the white atractylodes rhizome powder into water according to the weight ratio of the white atractylodes rhizome powder to the water of 1: 8-12, soaking for 2-4 hours, and extracting volatile oil under the reflux condition until the amount of the volatile oil is not increased any more; and (3) standing for 0.5-2 hours, separating the volatile oil at the upper layer and the water at the lower layer, and drying the volatile oil by adopting a drying agent to obtain the bighead atractylodes rhizome extract.

3. The nourishing moisturizer of claim 2, wherein:

the extraction is accomplished by means of a volatile oil extraction tank.

4. The nourishing moisturizer of claim 2, wherein:

the drying agent is selected from any one of anhydrous sodium sulfate, anhydrous magnesium sulfate and anhydrous calcium chloride.

5. The nourishing moisturizer of claim 1, wherein:

the white peony root extract is prepared by the following steps: cleaning and crushing white paeony root, and sieving with a 100-300-mesh sieve to obtain white paeony root powder; adding the white paeony root powder into an ethanol aqueous solution with the ethanol volume fraction of 60-80% according to the weight ratio of the white paeony root powder to the ethanol aqueous solution of 1: 5-10, carrying out reflux extraction for 2-4 times, extracting for 60-120 minutes each time, centrifuging after the extraction is finished, separating supernate, concentrating until no alcohol smell exists, adding ethyl acetate, extracting for 3-5 times, combining ethyl acetate phases, and concentrating to obtain the white paeony root extract.

6. The nourishing moisturizer of claim 1, wherein:

the glossy privet fruit extract is prepared by the following steps: cleaning and crushing glossy privet fruits, and sieving the glossy privet fruits by a sieve of 100-300 meshes to obtain glossy privet fruit powder; adding the glossy privet fruit powder into an ethanol aqueous solution with the volume fraction of ethanol being 60-80% according to the weight ratio of the glossy privet fruit powder to the ethanol aqueous solution being 1: 6-12, carrying out reflux extraction for 2-4 times, each time for 1-3 hours, centrifuging after the extraction is finished, separating supernate, concentrating until no alcohol smell exists, adding ethyl acetate, extracting for 3-5 times, combining ethyl acetate phases, and concentrating to obtain the glossy privet fruit extract.

7. A method of preparing the nourishing moisturizer of any of claims 1 to 6, comprising the steps of: firstly, mixing beta-sitosterol, squalene, vitamin E and an extract of bighead atractylodes rhizome according to the formula ratio, stirring and heating to 60-80 ℃ at the rotating speed of 100-500 rpm, and preserving heat for 10-30 minutes to obtain an oil phase component; mixing the white paeony root extract, the glossy privet fruit extract, the edetate disodium, the vitamin C, the Tween-80, the glycerol and the water according to the formula ratio, stirring and heating to 50-70 ℃ under the condition of the rotating speed of 100-500 rpm, and preserving the heat for 10-30 minutes to obtain a water phase component; and finally, adding the water phase component into the oil phase component, and emulsifying for 5-10 minutes at 50-60 ℃ under the condition of the rotating speed of 1000-3000 rpm to obtain the nourishing moisturizer.

8. The method of claim 7, wherein:

the stirring is accomplished by a mechanical or magnetic stirring device;

the emulsification is done by a homogenizer.