CN105998617B

CN105998617B - External traditional Chinese medicine extract with effect of relieving senile skin itch and preparation method and application thereof

Info

Publication number: CN105998617B
Application number: CN201610492915.8A
Authority: CN
Inventors: 鲁英伟; 孟宏
Original assignee: Tianjin Shengshi Yongye Science & Technology Co Ltd
Current assignee: Tianjin Shengshi Yongye Science & Technology Co Ltd
Priority date: 2016-06-24
Filing date: 2016-06-24
Publication date: 2019-12-13
Anticipated expiration: 2036-06-24
Also published as: CN105998617A

Abstract

The invention discloses an external traditional Chinese medicine extract with an effect of relieving senile skin itch as well as a preparation method and application thereof, and belongs to the technical field of cosmetic research. The invention relates to an external traditional Chinese medicine extract which is prepared by extracting the following raw materials in parts by weight: 15-50 parts of globeflower, 20-40 parts of divaricate saposhnikovia root, 10-30 parts of tree peony bark, 5-25 parts of dwarf lilyturf tuber, 10-20 parts of flos mume, 1-20 parts of mistletoe, 5-15 parts of Japanese groundsel herb and 1-20 parts of cornflower. Experiments prove that the product is safe and non-irritant to skin and has the effect of effectively relieving senile skin itch. The product of the invention can play a role in inhibiting inflammation by inhibiting hyaluronidase, can simultaneously fix and protect the skin barrier, and can instantly and long-term replenish skin moisture and prevent moisture loss, thereby achieving the effects of relieving itching, maintaining beauty and keeping young. The invention has the advantages of easy acquisition of raw materials, simple and convenient preparation method, greenness and safety, and good application prospect and market prospect.

Description

External traditional Chinese medicine extract with effect of relieving senile skin itch and preparation method and application thereof

Technical Field

The invention relates to an external traditional Chinese medicine extract, in particular to an external traditional Chinese medicine extract with the effect of relieving senile skin pruritus and a preparation method thereof, and also relates to an external skin care preparation prepared from the skin care composition. The invention belongs to the technical field of cosmetics research.

background

Skin problems such as dryness, itching and the like caused by daily necessities, external stimulation, alternate seasons and aging are increasingly paid more and more attention by markets and consumers, particularly the elderly. With the age, the skin barrier is damaged, sebum is overoxidized, the skin function is damaged or unbalanced, and symptoms such as tightness, stabbing pain, pruritus and the like are generated, and the daily life is affected in a serious condition. Therefore, solving the problem of skin itch is the key to skin care of the elderly.

in recent years, the traditional Chinese medicine extract serving as an additive of skin care products has the advantages of stable and lasting drug effect, remarkable curative effect, mild effect on skin, high safety and the like, and is popular and accepted by consumers.

based on the characteristics of the skin of the old people, the invention is based on the mechanism of causing the pruritus symptom of the old people, and multiple precious traditional Chinese medicinal materials such as globeflower, divaricate saposhnikovia root, tree peony bark, dwarf lilyturf tuber, flos mume, mistletoe, Chinese scholartree seed, cornflower and the like are compounded through scientific formula, so that the external traditional Chinese medicine extract is prepared.

disclosure of Invention

The invention aims to provide an external traditional Chinese medicine composition with the effect of relieving senile skin pruritus.

The invention also aims to provide an external traditional Chinese medicine extract prepared from the traditional Chinese medicine composition and a preparation method thereof.

The invention also aims to provide an external skin care preparation prepared on the basis of the traditional Chinese medicine extract.

in order to realize the purpose of the invention, the invention adopts the following technical scheme:

The invention utilizes advanced biotechnology, combines the traditional Chinese medicine theory of China, and effectively combines trollflower, divaricate saposhnikovia root, tree peony bark, dwarf lilyturf tuber, flos mume, mistletoe, Japanese wife fruit and cornflower together, and the dosage of the combination is determined, so as to form a compound. The brief introduction of the traditional Chinese medicine components is as follows:

Trollflower: is also named as herba Ecliptae and herba Pratentis Acutissimae, and is cold in nature; slightly bitter in taste; it enters heart meridian and has the functions of clearing away heat and toxic material.

Wind prevention: 2, pungent taste; sweet; mild sexual temperature; entering the bladder; a lung; a spleen; the liver meridian; dispelling wind and relieving exterior syndrome; eliminating dampness and relieving pain.

moutan bark: is pungent and bitter; cooling; slightly cold; returning to the heart; liver; a kidney; the lung meridian; clearing away heat; promoting blood circulation and removing blood stasis.

Radix ophiopogonis: sweet in nature; slightly bitter; is slightly cold. Mainly treats yin deficiency and lung dryness; cough with sticky sputum; deficiency of stomach yin; dry mouth and throat; constipation due to intestinal dryness.

and (3) green calyx plum: bitter, slightly sweet, slightly sour and cold in nature. Entering liver, stomach and lung meridians; soothing liver-qi stagnation, stimulating appetite, promoting salivation, dissipating phlegm

mistletoe: nature and taste: bitter; sweet; the nature is mild. Meridian tropism: liver; the kidney channel. Most provinces in China are produced in broad-leaved forests with the elevation of 500-1400 (-2000) meters, and parasitize elms, poplars, willows, birches, quercus, pears, plums, apples, Chinese ash, alder and basswood.

All-grass of Japanese creeper: pungent taste; slightly sweet; slightly cold; dispelling wind and removing dampness; clearing away heat and toxic material. Southern areas of Taiwan, Fujian, Guangdong, Guangxi, Yunnan provinces, etc.

Cornflower: compositae family Centaurea genus annual or biennial herb.

Specifically, the external traditional Chinese medicine composition with the effect of relieving senile skin pruritus is prepared from the following raw materials in parts by weight: 15-50 parts of globeflower, 20-40 parts of divaricate saposhnikovia root, 10-30 parts of tree peony bark, 5-25 parts of dwarf lilyturf tuber, 10-20 parts of flos mume, 1-20 parts of mistletoe, 5-15 parts of Japanese groundsel herb and 1-20 parts of cornflower.

In the present invention, preferably, the external traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of trollius chinensis, 20 parts of divaricate saposhnikovia root, 15 parts of tree peony bark, 15 parts of dwarf lilyturf tuber, 10 parts of flos mume, 20 parts of mistletoe, 5 parts of herb of veronica and 1 part of cornflower.

furthermore, the invention also provides an external traditional Chinese medicine extract with the effect of relieving senile skin pruritus, which is prepared from the traditional Chinese medicine composition by the following method:

(1) After all the raw materials are crushed, weighing all the raw materials according to the weight parts of the invention, and uniformly mixing;

(2) Adding water for extraction, wherein the mass ratio of the traditional Chinese medicine composition to the water is 5-25: extracting at 150-220 deg.c and 40-75 deg.c for 1-3 hr;

(3) Cooling the extracting solution obtained in the step (2) to 20-30 ℃, filtering by a 100-200 mesh sieve, filtering out dregs, performing vacuum filtration, and collecting filtrate.

In the invention, preferably, the mass ratio of the traditional Chinese medicine composition to water in the step (2) is 10-15: 180-200.

In the invention, preferably, the vacuum filtration in the step (3) comprises spreading two layers of filter paper in a buchner funnel, sandwiching 0.3-0.6 cm thick diatomite between the filter paper, and then carrying out vacuum filtration.

Furthermore, the invention also provides application of the external traditional Chinese medicine extract in preparing an external skin care preparation, wherein preferably, the external skin care preparation has the effects of relieving senile skin pruritus, resisting inflammation, preserving moisture and improving skin microcirculation.

The invention relates to an external skin care preparation with the effect of relieving senile skin itch, which is prepared from the external traditional Chinese medicine extract and conventional auxiliary materials in the field of skin care products.

specifically, the invention provides an emulsion with an effect of relieving senile skin pruritus, which consists of a phase A, a phase B and a phase C;

wherein phase A comprises PEG-100 glyceryl stearate/glyceryl stearate, cetearyl alcohol, pentaerythritol distearate, tocopheryl acetate, shea butter extract, hydrogenated polydecene, caprylic/capric triglyceride, isopropyl myristate, dimethicone, and cyclopentadimethylsiloxane/cyclotetrasiloxane;

Wherein the phase B comprises carbomer, glycerol, butanediol, disodium EDTA, allantoin, trehalose, alpha-mannan and water;

Wherein, the phase C comprises 10% (w/w) NaOH solution, the external traditional Chinese medicine extract, sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80 and methylisothiazolinone/iodopropynyl alcohol butyl carbamate;

Wherein the mass percentage of each raw material is as follows:

Preferably, the mass percentage of each raw material is as follows:

In the invention, preferably, the emulsion with the effect of relieving senile skin pruritus is prepared according to the following method:

(1) Mixing the raw materials of the phase A, heating to 75-85 ℃, uniformly stirring, and keeping the temperature for 20-60 minutes for later use;

(2) Fully dispersing and hydrating carbomer and water in the phase B, adding other raw materials in the phase B, heating to 75-85 ℃, uniformly stirring, and keeping the temperature for 20-60 minutes for later use;

(3) homogenizing the phase B, adding the phase A obtained in the step (1) into the phase B, and continuing homogenizing for 3-8 minutes;

(4) Stirring and cooling, wherein the cooling rate is 1-2 ℃/min, cooling to 45-50 ℃, adding 10% (w/w) NaOH solution, and stirring uniformly;

(5) adding the Chinese medicinal extract, and stirring; adding sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80, and stirring;

(6) Cooling to below 45 deg.C under stirring, adding methylisothiazolinone/iodopropynyl butylcarbamate, and stirring.

the preparation prepared by the raw materials and the method is emulsion. According to different using ways, the external traditional Chinese medicine composition can be extracted to obtain active extracting solution, and can be prepared into other external skin care preparations, such as cream, essence, cosmetic water, gel and the like, but the external traditional Chinese medicine composition is not limited to the above formulations, and can be prepared into various external preparations according to known methods in the field of skin care product industry.

The preparation method of the traditional Chinese medicine composition is not limited to the method, and other conventional methods in the field can achieve the effects, and the method is recommended by the inventor to be a better preparation method. The components of the formula of the skin care preparation of the invention act synergistically.

The recommended use method of the emulsion of the invention is as follows: after cleaning the face every day, applying the face lotion on the surface of human skin by a smearing method, and gently massaging until the face lotion is absorbed.

finally, the invention also provides the application of the emulsion with the effect of relieving the senile pruritus in relieving the senile pruritus, resisting inflammation, preserving moisture and improving skin microcirculation, and preferably, the applicable population of the emulsion is over 50 years old.

compared with the prior art, the invention has the beneficial effects that:

Experiments prove that the product is safe and non-irritant to skin, and has effective itching relieving and soothing effects. The product of the invention can play a role in inhibiting inflammation by inhibiting hyaluronidase, can simultaneously fix and protect the skin barrier, and can instantly and long-term replenish skin moisture and prevent moisture loss, thereby achieving the effects of relieving itching, maintaining beauty and keeping young. The invention has the advantages of easy acquisition of raw materials, simple and convenient preparation method, greenness and safety, and good application prospect and market prospect.

drawings

FIG. 1 shows the evaluation results of the antipruritic effect of the herb extracts prepared in example 1;

FIG. 2 shows the measurement results of the hyaluronidase inhibition rate of the herbal extracts prepared in example 1;

FIG. 3 is a result of measuring the hydration rate of the emulsion formulation prepared in example 4;

Fig. 4 is a result of observing the amount of blood perfusion of the chinese herbal medicine extract prepared in example 1.

Detailed Description

The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

all the medicinal materials used in the embodiment can be purchased from Chinese medicinal material group companies, and meet the 2010 edition standard of the Chinese people's republic of China pharmacopoeia. Other raw materials can be purchased commercially, the sources of the raw materials used in the present invention are shown in Table 1, and the names and manufacturers of the instruments used in the present invention are shown in Table 2.

TABLE 1

TABLE 2

Example 1 preparation of extract of the external use Chinese medicine of the present invention

(1) Pulverizing flos Trollii, radix Saposhnikoviae, cortex moutan, radix Ophiopogonis, flos Pruni mume, herba Visci, herba Veronicae Divaricatae and cornflower, respectively weighing the raw materials according to the following weight, and mixing;

30g of trollius chinensis, 20g of divaricate saposhnikovia root, 15g of tree peony bark, 15g of dwarf lilyturf tuber, 10g of flos mume, 20g of mistletoe, 5g of Japanese wife fruit and 1g of cornflower.

(2) adding water for extraction, wherein the mass ratio of the traditional Chinese medicine composition to the water is 10: extracting at 200 deg.C and 40 deg.C for 1 hr;

(3) Cooling the extracting solution obtained in the step (2) to 25 ℃, filtering the extracting solution through a 100-200-mesh sieve, filtering out dregs, performing vacuum filtration, and collecting filtrate to obtain the compound Chinese medicine preparation.

the vacuum filtration comprises the steps of paving two layers of filter paper in a Buchner funnel, and carrying out vacuum filtration by sandwiching 0.5cm thick diatomite between the filter paper.

Example 2 preparation of extract of the external use Chinese medicine of the present invention

15g of trollius chinensis, 40g of divaricate saposhnikovia root, 10g of tree peony bark, 25g of dwarf lilyturf tuber, 15g of flos mume, 10g of mistletoe, 15g of Japanese wife seed and 15g of cornflower.

(2) adding water for extraction, wherein the mass ratio of the traditional Chinese medicine composition to the water is 25: extracting at 150 and 55 deg.C for 2 hr;

(3) cooling the extracting solution obtained in the step (2) to 20 ℃, filtering the extracting solution through a 100-200-mesh sieve, filtering out dregs, performing vacuum filtration, and collecting filtrate to obtain the compound Chinese medicine preparation.

the vacuum filtration comprises the steps of paving two layers of filter paper in a Buchner funnel, and carrying out vacuum filtration by sandwiching 0.3cm thick diatomite between the filter paper.

Example 3 preparation of extract of the external use traditional Chinese medicine of the present invention

(1) Pulverizing flos Trollii, radix Saposhnikoviae, cortex moutan, radix Ophiopogonis, flos Pruni mume, herba Visci, herba Veronicae Dillenii and cornflower, respectively weighing the raw materials according to the following weight, and mixing well:

50g of trollius chinensis, 25g of divaricate saposhnikovia root, 30g of tree peony bark, 5g of dwarf lilyturf tuber, 20g of flos mume, 1g of mistletoe, 10g of herb of Chinese scholartree and 20g of cornflower.

(2) Adding water for extraction, wherein the mass ratio of the traditional Chinese medicine composition to the water is 5: extracting at 220 deg.C and 75 deg.C for 3 hr;

(3) cooling the extracting solution obtained in the step (2) to 30 ℃, filtering the extracting solution through a 100-200-mesh sieve, filtering out dregs, performing vacuum filtration, and collecting filtrate to obtain the compound Chinese medicine preparation.

The vacuum filtration comprises the steps of paving two layers of filter paper in a Buchner funnel, and carrying out vacuum filtration by sandwiching 0.6cm thick diatomite between the filter paper.

EXAMPLE 4 preparation of emulsion formulations of the invention

1. the components and the dosage are as follows:

the components and the amounts are shown in the following table 3:

TABLE 3

2. the preparation method comprises the following steps:

(1) mixing the raw materials in the phase A, heating to 80 ℃, uniformly stirring, and keeping the temperature for 50 minutes for later use;

(2) fully dispersing and hydrating carbomer and water in the phase B, adding other raw materials in the phase B, heating to 75 ℃, uniformly stirring, and keeping the temperature for 50 minutes for later use;

(3) Homogenizing the phase B, adding the phase A obtained in the step (1) into the phase B, and continuing homogenizing for 6 minutes;

(4) stirring and cooling, wherein the cooling rate is 1-2 ℃/min, cooling to 45 ℃, adding 10% (w/w) NaOH solution, and stirring uniformly;

(5) adding the external traditional Chinese medicine extract prepared in the example 1, and uniformly stirring; adding sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80, and stirring;

EXAMPLE 5 preparation of emulsion formulations of the invention

1. The components and the dosage are as follows:

the components and the amounts are shown in the following table 4:

TABLE 4

2. the preparation method comprises the following steps:

(1) mixing the raw materials in the phase A, heating to 75 ℃, uniformly stirring, and preserving heat for 20 minutes for later use;

(2) fully dispersing and hydrating carbomer and water in the phase B, adding other raw materials in the phase B, heating to 80 ℃, uniformly stirring, and keeping the temperature for 60 minutes for later use;

(3) homogenizing the phase B, adding the phase A obtained in the step (1) into the phase B, and continuing homogenizing for 3 minutes;

(4) stirring and cooling, wherein the cooling rate is 1-2 ℃/min, cooling to 48 ℃, adding 10% (w/w) NaOH solution, and stirring uniformly;

(5) adding the external traditional Chinese medicine extract prepared in the example 2, and uniformly stirring; adding sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80, and stirring;

EXAMPLE 6 preparation of emulsion formulations of the invention

1. the components and the dosage are as follows:

The components and the amounts are shown in the following table 5:

TABLE 5

2. The preparation method comprises the following steps:

(1) mixing the raw materials in the phase A, heating to 85 ℃, uniformly stirring, and preserving heat for 60 minutes for later use;

(2) fully dispersing and hydrating carbomer and water in the phase B, adding other raw materials in the phase B, heating to 85 ℃, uniformly stirring, and keeping the temperature for 20 minutes for later use;

(3) Homogenizing the phase B, adding the phase A obtained in the step (1) into the phase B, and continuing homogenizing for 8 minutes;

(4) Stirring and cooling, wherein the cooling rate is 1-2 ℃/min, cooling to 50 ℃, adding 10% (w/w) NaOH solution, and stirring uniformly;

(5) adding the external traditional Chinese medicine extract prepared in the embodiment 3, and uniformly stirring; adding sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80, and stirring;

(6) Cooling to below 45 deg.C under stirring, adding methylisothiazolinone/iodopropynyl butylcarbamate, and stirring to obtain the final product

Test example 1 evaluation of efficacy

First, evaluation of antipruritic and anti-inflammatory effects of the extract of the topical Chinese medicinal materials prepared in example 1

1. Histamine phosphate scratchiness threshold test

the experimental method comprises the following steps:

the method adopts an animal experiment of a rat skin itch model caused by histamine phosphate, and the effect of a sample on improving skin itch is demonstrated through observing the itch-causing effect of histamine phosphate with different concentrations on a mouse sample application part, so that the itch-relieving effect of a formula is verified.

24 rats are taken, half of each male and female, 350 +/-20 g. Freely drinking water, naturally illuminating at 20-24 ℃ and 40-60% of relative humidity. The dorsum of the foot was shaved 3 days before and 3 days after the experiment and randomly divided into 4 groups, i.e., experimental low dose group, experimental medium dose group, experimental high dose group, and water reference group. Each group had 6. An appropriate amount of histamine phosphate reference substance is precisely weighed and prepared into solutions with a series of concentrations of 0.01 wt%, 0.02 wt%, 0.03 wt% and 0.04 wt% … … for later use by using distilled water before use. Water reference group rats were each 0.1mL/1cm at the shaved site²uniformly coating with a certain amount of water, and mixing the Chinese medicinal extract with the test sample at a ratio of 0.15mL/1cm²、0.1mL/1cm²、0.05mL/1cm²The topical application can be used as a large-dose, medium-dose agentThe dose group and the low dose group.

the third day, the shaved area of the right hind instep was scratched with coarse sandpaper and the area was about 1cm²After the topical application of water, the topical Chinese medicinal extract prepared in example 1 was applied 1 time, 0.05ml of histamine phosphate 0.01% was dropped on the wound surface 10 minutes after the previous application, and then 0.05ml was dropped every 3 minutes at increasing concentrations of 0.01%, 0.02%, 0.03%, and 0.04% … …. Until the rat licks the right hind paw, the total amount of histamine phosphate given when the rat licks the right hind paw was last appeared was taken as the itch threshold (minimum itch causing amount of histamine phosphate smeared). The scratchiness threshold was calculated for each group and the differences between groups were compared.

the results are shown in FIG. 1. As can be seen from fig. 1, the itch causing threshold of the traditional Chinese medicine extract prepared in example 1 is obviously higher than that of the control group, which indicates that the traditional Chinese medicine extract prepared in the invention has good histamine phosphate resisting effect and obvious improvement effect on skin itch caused by histamine phosphate, i.e. the traditional Chinese medicine extract prepared in the invention has good itch relieving efficacy.

2. Example 1 inhibitory Effect of the extract of the Chinese medicinal materials for external use prepared in example 1 on Hyaluronidase

Preparing an experimental reagent:

(1) Acetic acid buffer solution (pH 5.6): measuring 1155 mu L of glacial acetic acid, diluting to 100mL, uniformly mixing, and taking 4.8mL as A solution; weighing 2.72g of sodium acetate crystal, adding water to dissolve the sodium acetate crystal, metering the volume to 100mL, uniformly mixing, and taking 45.2mL of the solution as a B solution; the solution A, B was mixed and made up to 100mL with water. Precisely measuring pH, and adjusting to 5.6 with solution A or B.

(2) Hyaluronidase solution: 0.0100g hyaluronidase was weighed into a beaker and 4mL of acetic acid buffer was added to the beaker to achieve a final working concentration of 1250 u/mL.

(3)0.5mg/mL sodium hyaluronate solution: 0.0050g of sodium hyaluronate was weighed and placed in a beaker, and 10mL of acetic acid buffer solution was added.

(4) ellichi reagent (Ehrlich reagent): 0.8g of p-dimethylaminobenzaldehyde is weighed and dissolved in 15mL of concentrated hydrochloric acid and 15mL of absolute ethyl alcohol, and the mixture can be stored for two months.

(5) Acetylacetone solution: 3.5mL of acetylacetone was dissolved in 50mL of 1.0mol/L sodium carbonate solution, and the solution was prepared before use.

(6) Traditional Chinese medicine extract diluent: the herb extracts prepared in example 1 were prepared into 1% (v/v), 2% (v/v) and 5% (v/v) dilutions of the herb extracts, respectively, and used for the following study.

The experimental method comprises the following steps:

the method is carried out by adopting an Elson-Morgan method for hyaluronidase in-vitro inhibition experiment. 0.1mL of 0.25mmol/L CaCl is taken₂the solution and 0.5mL of hyaluronidase solution are cultured for 20min at 37 ℃ in an insulation way; adding 0.5mL of Chinese medicinal extract diluent with concentration of 1%, 2% and 5%, respectively, and culturing at 37 deg.C for 20 min; adding 0.5mL sodium hyaluronate solution, keeping the temperature at 37 ℃ for 30min, and standing at normal temperature for 5 min; adding 0.1mL of 0.4mol/L NaOH solution and 0.5mL of acetylacetone solution, heating in a boiling water bath for 15min, and immediately cooling with ice water for 5 min; adding 1.0mL of Ellisib reagent, diluting with 3.0mL of anhydrous ethanol, standing for 20min for color development, and measuring the absorbance value with a spectrophotometer.

The hyaluronidase inhibition rate calculation formula is as follows:

In the formula: a-control ABS value (sample solution replaced by acetate buffer solution)

b-control blank solution ABS value (the sample solution and the enzyme solution are replaced by acetic acid buffer solution)

C-ABS value of the sample solution

D-ABS value of sample blank solution (acetic acid buffer solution is used to replace enzyme solution)

in the experiment, the wavelength scanning of a sample is firstly carried out in the range of 450-700nm to determine the maximum absorption wavelength, and then the ABS value is respectively measured at the maximum absorption wavelength by taking deionized water as a reference.

the hyaluronidase inhibition effect of each extract in vitro is shown in figure 2. As can be seen from fig. 2, the traditional Chinese medicine composition prepared in example 1 shows dose dependence on hyaluronidase inhibition, which indicates that the traditional Chinese medicine extract prepared in the invention has good inhibition effect on hyaluronidase, and thus has good anti-inflammatory effect.

Second, evaluation of efficacy of emulsion formulation prepared in example 4 in repairing skin barrier

The tested person: 20 Chinese women over 50 years of age with healthy skin, stopped using the skin care product 3 days before the test.

testing parts: the subject is inside the arm. The area to be tested (4 cm. times.4 cm) was marked 5cm from the base of the palm, multiple areas (1 cm apart) were marked simultaneously on the same arm, and the test samples were randomly distributed.

Test samples: the No. 1 control group was blank matrix (formula of matrix is shown in Table 3, and no Chinese medicinal extract was contained); experimental group # 2 is the emulsion formulation prepared in example 4.

And (3) testing environment: the indoor temperature is 22 +/-2 ℃, and the relative humidity is 50% +/-5%;

the test method comprises the following steps:

the subject voluntarily fills out the informed consent, cleans the test site, gently wipes, and sits still for 20 minutes.

A cyclic double blind labeling is used.

Starting the test, timing at 0 point, collecting the skin background value of the subject, and recording as T₀；

And (3) smearing a sample on the tested part by an experimenter, timing, and collecting data of the tested part in 1h, 2h and 4 h.

In order to timely understand and treat the adverse reactions which may occur after the test subject uses the sample or the control substance, the test subject can respond to the discomfort of the tested area at any time during the test period, for example, when obvious systemic adverse reactions or skin irritation of more than 3 grades occur, the test is immediately stopped, and the test subject is appropriately treated.

the test contents are as follows:

MMV value of skin moisture content

evaluation criteria:

The test averages the data measured 5 times in the same sample area of the subject and performs t-test analysis using SPSS statistical software. And making a conclusion whether the calculation result is valid or not.

As a result:

In the test period, no obvious systemic adverse reaction or skin irritation above grade 3 occurs in the tested population, so that the number of effective volunteers in the test is 20.

MMV value of skin moisture content

△＝Tn-T₀

Hydration rate ═ Tn-T₀)/T₀The higher the value, the more obvious the water replenishing effect.

in the formula: t0 is the sample set starting value;

Tn is the data collected for each time point for the sample set.

TABLE 6 statistical results of MMV values of skin moisture content before and after sample application

And (4) conclusion:

As can be seen from table 6, within 4h of the test period, the subjects showed a tendency to increase the skin hydration MMV values after using the sample, and from 1h there was a significant difference in MMV values (p <0.05) in the test area compared to before not using the sample.

The hydration rate is shown in FIG. 3. As can be seen from FIG. 3, within 4h of the test period, after the test subjects used the samples, the hydration rates of the test zones were all positive from 1h, indicating that the samples had the effect of replenishing skin moisture; wherein the hydration rate of the 2# sample is always higher than 1 #.

The inventors repeated the above experiments with the herbal extract or emulsion formulations prepared in example 1, example 2, example 3, example 5, and example 6 to arrive at the same conclusion. The test results show that the traditional Chinese medicine extract and the emulsion preparation prepared by the invention can obviously improve the moisture content of skin and have good skin-protecting barrier effect.

Thirdly, improving the skin metabolism-evaluating the effect of promoting the skin microcirculation

the skin microcirculation is a complex dynamic system and plays a very important role in skin color, temperature regulation, skin metabolism and transdermal transport. The blood circulation promoted by the skin microcirculation provides sufficient nutrients and nutrient substances for fibroblasts, collagen and the like of the skin, so that metabolites of cells and various harmful substances are removed in time, and the metabolism of the skin is ensured. When the blood volume in the human body is insufficient or the blood flow is not smooth, the complexion is dull and the pigmentation is caused.

The non-contact laser Doppler imaging instrument is adopted to test the face of a subject, and the change of the blood perfusion amount is observed so as to reflect the change condition of skin microcirculation before and after the product is used.

Testing parts: subject's left cheek

Test samples: the herbal extract prepared in example 1.

The test method comprises the following steps:

the subjects voluntarily filled out an informed consent, and the skin background value was tested before using the samples and again after 1 month of use. The test method comprises the steps of cleaning a tested part, lightly wiping, sitting still for 20 minutes, fixing the head, aligning the laser Doppler scanning lens to the left cheek, and testing the blood flow perfusion volume of the facial skin.

Blood perfusion images before and after the subjects used the samples are shown in fig. 4. As can be seen from fig. 4, after the subject uses the traditional Chinese medicine extract prepared in example 1, the blood perfusion amount of the facial skin is significantly increased, which indicates that the traditional Chinese medicine extract prepared in the invention has good effects of promoting skin microcirculation and improving skin metabolism. The inventors repeated the above experiments with the herbal extract or emulsion formulations prepared in example 2, example 3, example 4, example 5, and example 6 to arrive at the same conclusion.

Claims

1. A topical traditional Chinese medicine composition with the effect of relieving senile skin itch is characterized by comprising the following raw materials in parts by weight: 15-50 parts of globeflower, 20-40 parts of divaricate saposhnikovia root, 10-30 parts of tree peony bark, 5-25 parts of dwarf lilyturf tuber, 10-20 parts of flos mume, 1-20 parts of mistletoe, 5-15 parts of Japanese groundsel herb and 1-20 parts of cornflower.

2. The external traditional Chinese medicine composition as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 30 parts of trollius chinensis, 20 parts of divaricate saposhnikovia root, 15 parts of tree peony bark, 15 parts of dwarf lilyturf tuber, 10 parts of flos mume, 20 parts of mistletoe, 5 parts of herb of veronica and 1 part of cornflower.

3. An external traditional Chinese medicine extract with the effect of relieving senile pruritus, which is characterized by being prepared from the traditional Chinese medicine composition of claim 1 or 2 by the following method:

(1) After crushing the raw materials, weighing the raw materials according to the parts by weight of the raw materials in the claim 1 or 2, and uniformly mixing;

4. the external traditional Chinese medicine extract as claimed in claim 3, wherein the mass ratio of the traditional Chinese medicine composition to water in the step (2) is 10-15: 180-200.

5. the topical Chinese medicinal extract according to claim 3, wherein the vacuum filtration in step (3) comprises spreading two layers of filter paper in a Buchner funnel, sandwiching 0.3-0.6 cm thick diatomaceous earth between the filter paper, and vacuum filtering.

6. use of the extract of the external traditional Chinese medicine according to any one of claims 3 to 5 for the preparation of an external skin care preparation.

7. the use according to claim 6, wherein the skin care preparation for external use has effects of relieving senile skin pruritus, anti-inflammatory, moisturizing and improving skin microcirculation.

8. A skin care preparation for external use with the effect of relieving senile pruritus, which is characterized in that the skin care preparation is prepared from the traditional Chinese medicine extract for external use according to any one of claims 3 to 5 and conventional auxiliary materials in the field of skin care products.

9. An emulsion with the effect of relieving senile skin pruritus is characterized by consisting of a phase A, a phase B and a phase C;

Wherein the phase C comprises a 10% (w/w) NaOH solution, the topical herbal extract of any one of claims 3-5, sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80, and methylisothiazolinone/iodopropynyl alcohol butyl carbamate;

Wherein the mass percentage of each raw material is as follows:

10. The emulsion according to claim 9, characterized in that the mass percentages of the raw materials are as follows:

11. the emulsion with the effect of relieving senile skin pruritus as claimed in claim 9, which is prepared according to the following method:

(5) adding the external traditional Chinese medicine extract, and stirring uniformly; adding sodium acrylate/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80, and stirring;

12. Use of the emulsion having an effect of relieving senile skin pruritus according to any one of claims 9 to 11 for preparing an external skin care preparation for relieving senile skin pruritus, resisting inflammation, moisturizing and improving skin microcirculation.

13. The use according to claim 12, wherein the topical skin care formulation is suitable for use in a population over 50 years of age.