CN107896793A - A kind of method of masson pine seed nursery inoculation India truffle - Google Patents
A kind of method of masson pine seed nursery inoculation India truffle Download PDFInfo
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- CN107896793A CN107896793A CN201711226379.8A CN201711226379A CN107896793A CN 107896793 A CN107896793 A CN 107896793A CN 201711226379 A CN201711226379 A CN 201711226379A CN 107896793 A CN107896793 A CN 107896793A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention discloses a kind of method of masson pine seed nursery inoculation India truffle, belongs to wild-type strain planting technology field.The pretreatment that carries out disinfection first to pinus yunnanensis seed is spare, select fresh India truffle to be equipped with sterile water after crushing and obtain ferfas strain liquid, be uniformly mixed by the weight ratio feeding of dry pond sludge 20 30%, gold zone mud 20 30%, pine tree mycorhiza soil 20 30%, river sand 5 15%, vermiculite 10 20% and adjusted moisture content to obtain inoculation matrix fine powder spare；Then the seedbed for being filled with inoculation matrix is set in greenhouse and equidistantly uniformly insert out dibbling cave in inoculation matrix, and pretreated masson pine seed vernalization is substantially stretched out to plumule, the downward dibbling of bud root again enters in the dibbling cave of inoculation in the seedbed matrix and the fine woods soil of bedding, then carry out seedling management, seed goes out after leaf monthly to apply seedling ferfas strain liquid 23 times, until seedling grows up to mycorhiza sapling.There is method is reliable, rate of vaccination is high, cost is low etc..
Description
Technical field
The present invention relates to a kind of India truffle inoculation method, especially a kind of masson pine seed nursery inoculation India truffle
Method, belongs to wild-type strain planting technology field.
Background technology
India truffle (Tuber indicum), also known as truffle, popular name pig encircle bacterium, without ma fruit etc., are referred to as " the world first
Name bacterium ".India truffle is rich in 17 kinds of amino acid, 8 kinds of vitamins, suitable protein and androsterone, sterol, sphingolipid, fat
Acid, amino acid and trace element etc. more than 50 plants physiologically active ingredient, and cannot be synthesized containing human body itself 8 kinds of amino acid, zinc,
The essential nutrients such as manganese, iron, calcium, phosphorus, selenium, with medicinal valencys such as strengthen immunity, anti-aging, beneficial stomach, clear god, hemostasis, treatment hemorrhoid
Value, has active anticancer, there is certain inhibitory action to cancer cell, can excite brain cell activity.Due to wild India truffle
Low output by artificial cultivation, it is necessary to meet ever-increasing market demand, and effective inoculation method is not only India's block
The premise of bacterium Mycorrhizal production, and the key of ferfas cultivation success or failure.
Masson pine（Pinus massoniana Lamb.）It is Pinaceae Pinus arbor, is called pine, illawarra mountain pine, fir pine etc.,
There is certain distribution in China Qinling Mountains Huaihe River, Yunnan, masson pine be not only in industrial and agricultural production it is important use material, while it be also
The very high plant of medicinal upper value, main function are energy wind-damp dispelling, and promoting blood circulation is dispelled more, analgesic, hemostasis etc..
At present, though having the cultivation inoculation technique on India truffle on a small quantity both at home and abroad, and apply to a certain extent manually
In cultivation practices, but rest on the exploratory stage, technology is also remote immature more.Huashan pine as India truffle host plant it
One, attract attention in the artificial cultivation of India truffle, but existing Huashan pine inoculation technique is still immature, and effect of inoculation is paid no attention to
Think, success ratio of inoculation is relatively low, causes the cost increase of India truffle cultivation, yield to be limited, it is difficult to promote and apply, it is necessary to study
The Huashan pine India truffle Inoculation Method of effect.
The content of the invention
The technical problem to be solved in the present invention is low, of high cost for success ratio of inoculation in existing India truffle artificial cultivation
The deficiencies of, propose a kind of method of masson pine seed nursery inoculation India truffle, by host plant seedling culture and build bacterium
Kind growing environment, improves India truffle success ratio of inoculation, reduces production cost.
In order to solve the above technical problems, the present invention provides a kind of method of masson pine seed nursery inoculation India truffle, its
Comprise the following steps that：
（1）Seeds preprocess：The seed of formed objects specification is selected, first the soaking disinfection 1-2 in 0.5-1% liquor potassic permanganates
Hour, take out and use aseptic water washing 2-3 times, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
Wash 2-3 times and be placed on the smalls shape that granularity 50-80 mesh is crushed in pulverizer, then by 1：The volume ratio of 5-10 adds sterile water,
It is spare to obtain ferfas strain liquid；
（3）Prepare inoculation matrix：By dry pond sludge 20-30%, gold zone mud 20-30%, pine tree mycorhiza soil 20-30%, river sand 5-15%, leech
The weight ratio of stone 10-20%, takes dry pond sludge, gold zone mud, vermiculite, river sand, is crushed to granularity less than or equal to after 4mm and loose
Pine tree mycorhiza soil be uniformly mixed, and it is 10-20% to adjust moisture content（Add suitable water）, it is spare to obtain inoculation matrix；
（4）Preparing nursery bed：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed
Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed, and with bamboo stick in inoculation in the seedbed matrix it is equidistant
The even dibbling cave for inserting out 0.2-0.3cm depths；
（5）Presprouting of seeds：By step（1）In pretreated masson pine seed be put into step（2）The India truffle bacterium prepared
2-4h is soaked in kind liquid, then seed is taken out and is placed on vernalization according to a conventional method under 25-35 DEG C of environment, until masson pine seed is grinned
And plumule has stretched out 0.15-0.2cm；Masson pine seed is sprayed once with 30-40 DEG C of warm water daily in pregermination procedure, and
Keep masson pine seed surface moist；
（6）Seed is sowed：Take step（5）The masson pine seed that the plumule of acquisition has stretched out, enters step by its downward dibbling of bud root
（4）In the dibbling cave of ready inoculation in the seedbed matrix, then above, the fine woods of one layer of bedding is native and squirts table soil with water；
（7）Mycorrhiza fungi seeding cultivating：It is aperiodically right in temperature, humidity, the suitable germination of illuminance and the greenhouse of growth
Seedbed watering, keep the table soil of matrix not split, and monthly nursery loosen the soil for 1-2 times and weeding, and germination is unearthed seedling
Seedling is applied according to a conventional method afterwards with mycorhiza fertilizer；Seed, which grows to start monthly to pour seedling after leaf, applies step（2）The block of preparation
Bacterium strain liquid 2-3 times, until seedling is grown to height of seedling 100-120cm, obtains masson pine truffle mycorrhiza fungi sapling.
The step（3）The pH value of the inoculation matrix of preparation is 6.7-7.5（Adjusted using conventional method）, be preferably it is slightly sour extremely
Neutrality, in favor of the growth of the emergence of Huashan pine clock, growth and strain.
The step（3）The masson pine of India truffle is associated with the middle desirable pine forest of pine tree mycorhiza soil for preparing inoculation matrix
Topsoil under tree uses, to ensure well-grown after strain inoculation.Conifer nursery cannot lack VA Mycorrhizal Fungi, lack VA Mycorrhizal Fungi
It can cause undergrowth after being inoculated with.
The step（4）The length and width in middle seedbed, it is deep be respectively 2-3m, 1-1.5m, 1-1.5m, the wide 40- of pavement between seedbed
50cm。
The step（4）The density of dibbling cave point is every square metre of 300-350 in middle inoculation in the seedbed matrix.
The step（6）The sowing time of middle masson pine seed preferably in the annual 3-4 months, with improve the emergence rate of seed and
The effect of inoculation.
The step（6）The fine woods soil of middle bedding is the topsoil in common masson pine forest, after sterilized sterilizing, powder
It is used below to be broken to granularity 2mm；The general thickness 5-10mm of fine woods soil of institute's bedding.
The step（7）In medium temperature chamber's greenhouse suitable germination and growth temperature be 25-30 DEG C, humidity 75-
85%th, illuminance 30000-35000ux.
It is each that the composition proportion of matrix, inoculation time, density, greenhouse temperature and the humidity etc. of dibbling cave point are inoculated with the present invention
Parameter can be specific definite according to being actually needed, and ensures good effect of inoculation.
Inoculation matrix and special inoculation method for culturing seedlings of the present invention using appropriate component, pass through the multiple strain of too many levels
Charity, for India truffle strain masson pine sapling root place life built good environment, substantially increase inoculation into
Power, reduces the cost of artificial growth India truffle, and the rate of vaccination of India truffle can be made to reach more than 82%.Can with method
Lean on, the advantage such as rate of vaccination is high, cost is low, be suitably widely popularized use.
Embodiment
The embodiment of the present invention is further described in detail below.The technology being not specified in embodiment or production
Product are the prior art or can be by buying the conventional products obtained.
Embodiment 1：The method that the nursery of this masson pine seed is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：The seed of formed objects specification is selected, when first soaking disinfection 1 is small in 0.5% liquor potassic permanganate,
Take out and use aseptic water washing 2 times, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
2 times smalls shapes for being placed on 50 mesh of grinding particle size in pulverizer are washed, then by 1：10 volume ratio adds sterile water, obtains ferfas bacterium
Kind liquid is spare；
（3）Prepare inoculation matrix：By dry pond sludge 20%, gold zone mud 20%, pine tree mycorhiza soil 30%, river sand 10%, vermiculite 20% weight
Than taking dry pond sludge, gold zone mud, vermiculite, river sand, being crushed to granularity and mixed with loose pine tree mycorhiza soil less than after 2mm
It is even, and adjust moisture content be 10%, pH value 6.7, obtain inoculation matrix it is spare；The pine tree mycorhiza soil for preparing inoculation matrix is pine
The topsoil being associated with woods under the horse hair pine tree of India truffle, to ensure well-grown after strain inoculation；
（4）Seedbed preparation：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed
Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed, and with bamboo stick in inoculation in the seedbed matrix it is equidistant
The even dibbling cave for inserting out 2mm depths；The length and width in seedbed, it is deep be respectively 2m, 1m, 1m, the wide 40cm of pavement between seedbed；Inoculation in the seedbed
The density of dibbling cave point is 300 every square metre in matrix；
（5）Presprouting of seeds：By step（1）In pretreated masson pine seed be put into step（2）The India truffle bacterium prepared
4h is soaked in kind liquid, then seed is taken out and is placed on vernalization according to a conventional method under 25 DEG C of environment, until masson pine seed is grinned and embryo
Bud has stretched out 1.5mm；Masson pine seed is sprayed once with 30 DEG C of warm water daily in pregermination procedure, and keeps masson pine kind
Sublist face is moist；
（6）Seed is sowed：Take step（5）The masson pine seed that the plumule of acquisition has stretched out, enters step by its downward dibbling of bud root
（4）In the dibbling cave of ready inoculation in the seedbed matrix, then above, the fine woods of one layer of bedding is native and squirts table soil with water；Paving
The fine woods soil of lid is the topsoil in common masson pine forest, after sterilized sterilizing, is crushed to granularity 2mm uses；Institute's bedding
The native thickness 5mm of fine woods.The sowing time of masson pine seed in annual March, to improve the effect of the emergence rate of seed and inoculation
Fruit；
（7）Mycorrhiza fungi seeding cultivating：25 DEG C of temperature, humidity 75%, illuminance 30000lux in greenhouse are controlled, irregularly to seedbed
Watering, keep the table soil of matrix not split, and monthly nursery loosen the soil for 1 time and weeding, germination are unearthed after seedling by normal
Rule method is applied seedling with mycorhiza fertilizer；Seed, which grows to start after leaf to pour seedling every month, applies step（2）The ferfas bacterium of preparation
Kind liquid 2 times, until seedling is grown to height of seedling 100cm, that is, obtains masson pine truffle mycorrhiza fungi sapling.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 82%, enters harvest within the 9th year after plantation
Phase, the per unit area yield of ferfas brings up to 8kg/ mus by the 2.5kg/ mus of existing method, and ferfas promotes the growth and development of masson pine, together
When masson pine effectively promote again ferfas growth and development improve plant diseases and insect pests resistance, can keep and stablize ecosystem
System, promotes the recovery and development of forest cover.
Embodiment 2：The method that the nursery of this masson pine seed is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：The seed of formed objects specification is selected, first soaking disinfection 1.5 is small in 0.8% liquor potassic permanganate
When, take out and use aseptic water washing 2 times, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
3 times smalls shapes for being placed on 70 mesh of grinding particle size in pulverizer are washed, then by 1：7 volume ratio adds sterile water, obtains ferfas bacterium
Kind liquid is spare；
（3）Prepare inoculation matrix：By dry pond sludge 25%, gold zone mud 25%, pine tree mycorhiza soil 25%, river sand 15%, vermiculite 10% weight
Than taking dry pond sludge, gold zone mud, vermiculite, river sand, being mixed with loose pine tree mycorhiza soil after being crushed to below granularity 3mm
It is even, and adjust moisture content be 15%, pH value 7, obtain inoculation matrix it is spare；The pine tree mycorhiza soil for preparing inoculation matrix is pine forest
In be associated with topsoil under the horse hair pine tree of India truffle, to ensure well-grown after strain inoculation；
（4）Seedbed preparation：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed
Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed, and with bamboo stick in inoculation in the seedbed matrix it is equidistant
The even dibbling cave for inserting out 3mm depths；The length and width in seedbed, it is deep be respectively 3m, 1.5m, 1.5m, the wide 45cm of pavement between seedbed；Seedbed
The density for being inoculated with dibbling cave point in matrix is 320 every square metre；
（5）Presprouting of seeds：By step（1）In pretreated masson pine seed be put into step（2）The India truffle bacterium prepared
3h is soaked in kind liquid, then seed is taken out and is placed on vernalization according to a conventional method under 30 DEG C of environment, until masson pine seed is grinned and embryo
Bud has stretched out 2mm；Masson pine seed is sprayed once with 35 DEG C of warm water daily in pregermination procedure, and keeps masson pine seed
Surface is moist；
（6）Seed is sowed：Take step（5）The masson pine seed that the plumule of acquisition has stretched out, enters step by its downward dibbling of bud root
（4）In the dibbling cave of ready inoculation in the seedbed matrix, then above, the fine woods of one layer of bedding is native and squirts table soil with water；Paving
The fine woods soil of lid is the topsoil in common masson pine forest, after sterilized sterilizing, is crushed to granularity 1.5mm uses；Spread
The native thickness 8mm of the fine woods of lid.The sowing time of masson pine seed in annual April, to improve the emergence rate of seed and inoculation
Effect；
（7）Mycorrhiza fungi seeding cultivating：30 DEG C of temperature, humidity 80%, illuminance 31000lux in greenhouse are controlled, irregularly to seedbed
Watering, keep the table soil of matrix not split, and monthly nursery loosen the soil for 2 times and weeding, germination are unearthed after seedling by normal
Rule method is applied seedling with mycorhiza fertilizer；Seed, which grows to start after leaf to pour seedling every month, applies step（2）The ferfas bacterium of preparation
Kind liquid 3 times, until seedling is grown to height of seedling 110cm, that is, obtains masson pine truffle mycorrhiza fungi sapling.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 84%, enters harvest within the 6th year after plantation
Phase, the per unit area yield of ferfas brings up to 8.5kg/ mus by the 2.5kg/ mus of existing method, and ferfas promotes the growth and development of masson pine,
Masson pine effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again at the same time, can keep with stablizing ecology
System, promotes the recovery and development of forest cover.
Embodiment 3：The method that the nursery of this masson pine seed is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：The seed of formed objects specification is selected, when first soaking disinfection 2 is small in 1% liquor potassic permanganate, is taken
Go out to use aseptic water washing 3 times, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
3 times smalls shapes for being placed on 80 mesh of grinding particle size in pulverizer are washed, then by 1：10 volume ratio adds sterile water, obtains ferfas bacterium
Kind liquid is spare；
（3）Prepare inoculation matrix：By dry pond sludge 30%, gold zone mud 30%, pine tree mycorhiza soil 20%, river sand 5%, vermiculite 15% weight
Than taking dry pond sludge, gold zone mud, vermiculite, river sand, being mixed with loose pine tree mycorhiza soil after being crushed to below granularity 4mm
It is even, and adjust moisture content be 20%, pH value 7.5, obtain inoculation matrix it is spare；The pine tree mycorhiza soil for preparing inoculation matrix is pine
The topsoil being associated with woods under the horse hair pine tree of India truffle, to ensure well-grown after strain inoculation；
（4）Seedbed preparation：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed
Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed, and with bamboo stick in inoculation in the seedbed matrix it is equidistant
The even dibbling cave for inserting out 2.5mm depths；The length and width in seedbed, it is deep be respectively 2.5m, 1.2m, 1.3m, the wide 50cm of pavement between seedbed；
The density of dibbling cave point is 350 every square metre in inoculation in the seedbed matrix；
（5）Presprouting of seeds：By step（1）In pretreated masson pine seed be put into step（2）The India truffle bacterium prepared
4h is soaked in kind liquid, then seed is taken out and is placed on vernalization according to a conventional method under 35 DEG C of environment, until masson pine seed is grinned and embryo
Bud has stretched out 1.8mm；Masson pine seed is sprayed 2 times with 40 DEG C of warm water daily in pregermination procedure, and keeps masson pine kind
Sublist face is moist；
（6）Seed is sowed：Take step（5）The masson pine seed that the plumule of acquisition has stretched out, enters step by its downward dibbling of bud root
（4）In the dibbling cave of ready inoculation in the seedbed matrix, then above, the fine woods of one layer of bedding is native and squirts table soil with water；Paving
The fine woods soil of lid is the topsoil in common masson pine forest, and after sterilized sterilizing, it is used below to be crushed to granularity 2mm；Institute
The native thickness 10mm of the fine woods of bedding.The sowing time of masson pine seed in annual March, to improve the emergence rate of seed and inoculation
Effect；
（7）Mycorrhiza fungi seeding cultivating：28 DEG C of temperature, humidity 85%, illuminance 35000lux in greenhouse are controlled, irregularly to seedbed
Watering, keep the table soil of matrix not split, and monthly nursery loosen the soil for 2 times and weeding, germination are unearthed after seedling by normal
Rule method is applied seedling with mycorhiza fertilizer；Seed, which grows to start after leaf to pour seedling every month, applies step（2）The ferfas bacterium of preparation
Kind liquid 3 times, until seedling is grown to height of seedling 120cm, that is, obtains masson pine truffle mycorrhiza fungi sapling.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 86%, enters harvest within the 4th year after plantation
Phase, the per unit area yield of ferfas brings up to 9kg/ mus by the 2.5kg/ mus of existing method, and ferfas promotes the growth and development of masson pine, together
When masson pine effectively promote again ferfas growth and development improve plant diseases and insect pests resistance, can keep and stablize ecosystem
System, promotes the recovery and development of forest cover.
Embodiment 4：The method that the nursery of this masson pine seed is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：The seed of formed objects specification is selected, first soaking disinfection 1.5 is small in 0.6% liquor potassic permanganate
When, take out and use aseptic water washing 2 times, be placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
3 times smalls shapes for being placed on 65 mesh of grinding particle size in pulverizer are washed, then by 1：6 volume ratio adds sterile water, obtains ferfas bacterium
Kind liquid is spare；
（3）Prepare inoculation matrix：By dry pond sludge 28%, gold zone mud 24%, pine tree mycorhiza soil 23%, river sand 12%, vermiculite 13% weight
Than taking dry pond sludge, gold zone mud, vermiculite, river sand, being mixed after being crushed to below granularity 2.5mm with loose pine tree mycorhiza soil
Uniformly, and adjust moisture content be 13%, pH value 7.1, obtain inoculation matrix it is spare；Preparing the pine tree mycorhiza soil of inoculation matrix is
The topsoil being associated with pine forest under the horse hair pine tree of India truffle, to ensure well-grown after strain inoculation；
（4）Seedbed preparation：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed
Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed, and with bamboo stick in inoculation in the seedbed matrix it is equidistant
The even dibbling cave for inserting out 3mm depths；The length and width in seedbed, it is deep be respectively 3m, 1.5m, 1.5m, the wide 50cm of pavement between seedbed；Seedbed
The density for being inoculated with dibbling cave point in matrix is 330 every square metre；
（5）Presprouting of seeds：By step（1）In pretreated masson pine seed be put into step（2）The India truffle bacterium prepared
3.5h is soaked in kind of liquid, then seed is taken out and is placed on vernalization according to a conventional method under 30 DEG C of environment, until masson pine seed grin and
Plumule has stretched out 2mm；Masson pine seed is sprayed once with 35 DEG C of warm water daily in pregermination procedure, and keeps masson pine kind
Sublist face is moist；
（6）Seed is sowed：Take step（5）The masson pine seed that the plumule of acquisition has stretched out, enters step by its downward dibbling of bud root
（4）In the dibbling cave of ready inoculation in the seedbed matrix, then above, the fine woods of one layer of bedding is native and squirts table soil with water；Paving
The fine woods soil of lid is the topsoil in common masson pine forest, and after sterilized sterilizing, it is used below to be crushed to granularity 2mm；Institute
The native thickness 6mm of the fine woods of bedding.The sowing time of masson pine seed in annual March, to improve the emergence rate of seed and inoculation
Effect；
（7）Mycorrhiza fungi seeding cultivating：28 DEG C of temperature, humidity 80%, illuminance 32000lux in greenhouse are controlled, irregularly to seedbed
Watering, keep the table soil of matrix not split, and monthly nursery loosen the soil for 2 times and weeding, germination are unearthed after seedling by normal
Rule method is applied seedling with mycorhiza fertilizer；Seed, which grows to start after leaf to pour seedling every month, applies step（2）The ferfas bacterium of preparation
Kind liquid 3 times, until seedling is grown to height of seedling 120cm, that is, obtains masson pine truffle mycorrhiza fungi sapling.
The present embodiment is by using the method for the present invention, and the rate of vaccination of India truffle is 89%, and 4-9, which enters, after plantation receives
The phase is obtained, the per unit area yield of ferfas brings up to 9kg/ mus by the 2.5kg/ mus of existing method, and ferfas promotes the growth and development of masson pine,
Masson pine effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again at the same time, can keep with stablizing ecology
System, promotes the recovery and development of forest cover.
The technology contents of the present invention are described above, but protection scope of the present invention is not limited to the content,
, can also be on the premise of present inventive concept not be departed to this hair in the knowledge that those of ordinary skill in the art possess
Bright technology contents make a variety of changes, within the spirit and principles of the invention, any modification for being made, equivalent substitution,
Improve etc., it should all be included in the protection scope of the present invention.
Claims (9)
- A kind of 1. method of masson pine seed nursery inoculation India truffle, it is characterised in that comprise the following steps that：（1）Seeds preprocess：The seed of formed objects specification is selected, first the soaking disinfection 1-2 in 0.5-1% liquor potassic permanganates Hour, take out and use aseptic water washing 2-3 times, be placed in spare in culture dish；（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water Wash 2-3 times and be placed on the smalls shape that granularity 50-80 mesh is crushed in pulverizer, then by 1：The volume ratio of 5-10 adds sterile water, It is spare to obtain ferfas strain liquid；（3）Prepare inoculation matrix：By dry pond sludge 20-30%, gold zone mud 20-30%, pine tree mycorhiza soil 20-30%, river sand 5-15%, leech The weight ratio of stone 10-20%, takes dry pond sludge, gold zone mud, vermiculite, river sand, is crushed to granularity less than or equal to after 4mm and loose Pine tree mycorhiza soil be uniformly mixed, and adjust moisture content to 10-20%, it is spare to obtain inoculation matrix；（4）Seedbed preparation：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed, and with bamboo stick in inoculation in the seedbed matrix it is equidistant The even dibbling cave for inserting out 0.2-0.3cm depths；（5）Presprouting of seeds：By step（1）In pretreated masson pine seed be put into step（2）The India truffle bacterium prepared 2-4h is soaked in kind liquid, then seed is taken out and is placed on vernalization according to a conventional method under 25-35 DEG C of environment, until masson pine seed is grinned And plumule has stretched out 0.15-0.2cm；Masson pine seed is sprayed 1-2 times with 30-40 DEG C of warm water daily in pregermination procedure, And keep masson pine seed surface moist；（6）Seed is sowed：Take step（5）The masson pine seed that the plumule of acquisition has stretched out, enters step by its downward dibbling of bud root （4）In the dibbling cave of ready inoculation in the seedbed matrix, then above, the fine woods of one layer of bedding is native and squirts table soil with water；（7）Mycorrhiza fungi seeding cultivating：It is aperiodically right in temperature, humidity, the suitable germination of illuminance and the greenhouse of growth Seedbed watering, keep the table soil of matrix not split, and monthly nursery loosen the soil for 1-2 times and weeding, and germination is unearthed seedling Seedling is applied according to a conventional method afterwards with mycorhiza fertilizer；Seed, which grows to start monthly to pour seedling after leaf, applies step（2）The block of preparation Bacterium strain liquid 2-3 times, until seedling is grown to height of seedling 100-120cm, obtains masson pine truffle mycorrhiza fungi sapling.
- 2. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （3）The pH value of the inoculation matrix of preparation is 6.7-7.5, is preferably slightly sour to neutral.
- 3. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （3）In, the pine tree mycorhiza soil for preparing inoculation matrix is the topsoil being associated with pine forest under the horse hair pine tree of India truffle.
- 4. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （4）In, the length and width in seedbed, it is deep be respectively 2-3m, 1-1.5m, 1-1.5m, the wide 40-50cm of pavement between seedbed.
- 5. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （4）In, the density of dibbling cave point is every square metre of 300-350 in inoculation in the seedbed matrix.
- 6. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （6）In, the sowing time of masson pine seed is preferably in the annual 3-4 months.
- 7. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （6）In, the fine woods of bedding soil is the topsoil in common masson pine forest, after sterilized sterilizing, be crushed to 2 mm of granularity with Lower use.
- 8. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （6）In, the general thickness 5-10mm of fine woods soil of bedding.
- 9. the method for masson pine seed nursery inoculation India truffle according to claim 1, it is characterised in that：The step （7）In, in greenhouse suitable germination and growth temperature be 25-30 DEG C, humidity 75-85%, illuminance 30000- 35000lux。
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Citations (4)
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| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN105393873A (en) * | 2015-11-11 | 2016-03-16 | 黎平县枞集林农发展有限责任公司 | Cultivation method for masson pine seedlings |
| CN106134904A (en) * | 2016-07-28 | 2016-11-23 | 杭州凌萤科技有限公司 | Pinus massoniana Lamb fast seedling-cultivating method |
| CN106234112A (en) * | 2016-07-28 | 2016-12-21 | 杭州凌萤科技有限公司 | Pinus massoniana Lamb high efficiency seedling cultivating method |
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| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN105393873A (en) * | 2015-11-11 | 2016-03-16 | 黎平县枞集林农发展有限责任公司 | Cultivation method for masson pine seedlings |
| CN106134904A (en) * | 2016-07-28 | 2016-11-23 | 杭州凌萤科技有限公司 | Pinus massoniana Lamb fast seedling-cultivating method |
| CN106234112A (en) * | 2016-07-28 | 2016-12-21 | 杭州凌萤科技有限公司 | Pinus massoniana Lamb high efficiency seedling cultivating method |
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