CN107849603B - 利用有限核苷酸组成的引物扩增 - Google Patents

利用有限核苷酸组成的引物扩增 Download PDF

Info

Publication number
CN107849603B
CN107849603B CN201680036556.3A CN201680036556A CN107849603B CN 107849603 B CN107849603 B CN 107849603B CN 201680036556 A CN201680036556 A CN 201680036556A CN 107849603 B CN107849603 B CN 107849603B
Authority
CN
China
Prior art keywords
primer
primers
amplification
nucleotide
reverse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201680036556.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN107849603A (zh
Inventor
王友祥
杨志杰
陈欣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Atila BioSystems Inc
Original Assignee
Atila BioSystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atila BioSystems Inc filed Critical Atila BioSystems Inc
Priority to CN202111644199.8A priority Critical patent/CN114250274A/zh
Publication of CN107849603A publication Critical patent/CN107849603A/zh
Application granted granted Critical
Publication of CN107849603B publication Critical patent/CN107849603B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/143—Modifications characterised by incorporating a promoter sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201680036556.3A 2015-04-24 2016-04-22 利用有限核苷酸组成的引物扩增 Active CN107849603B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111644199.8A CN114250274A (zh) 2015-04-24 2016-04-22 利用有限核苷酸组成的引物扩增

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562152756P 2015-04-24 2015-04-24
US62/152,756 2015-04-24
PCT/US2016/029054 WO2016172632A2 (en) 2015-04-24 2016-04-22 Amplification with primers of limited nucleotide composition

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202111644199.8A Division CN114250274A (zh) 2015-04-24 2016-04-22 利用有限核苷酸组成的引物扩增

Publications (2)

Publication Number Publication Date
CN107849603A CN107849603A (zh) 2018-03-27
CN107849603B true CN107849603B (zh) 2022-01-28

Family

ID=57143585

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201680036556.3A Active CN107849603B (zh) 2015-04-24 2016-04-22 利用有限核苷酸组成的引物扩增
CN202111644199.8A Pending CN114250274A (zh) 2015-04-24 2016-04-22 利用有限核苷酸组成的引物扩增

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202111644199.8A Pending CN114250274A (zh) 2015-04-24 2016-04-22 利用有限核苷酸组成的引物扩增

Country Status (8)

Country Link
US (2) US11091799B2 (https=)
EP (1) EP3286338B1 (https=)
JP (2) JP6966681B2 (https=)
CN (2) CN107849603B (https=)
AU (2) AU2016252998B2 (https=)
CA (1) CA2983819A1 (https=)
ES (1) ES2961374T3 (https=)
WO (1) WO2016172632A2 (https=)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2633071B1 (en) 2010-10-27 2016-10-12 President and Fellows of Harvard College Compositions of toehold primer duplexes and methods of use
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
CA2983819A1 (en) 2015-04-24 2016-10-27 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
EP3532635B1 (en) * 2016-10-31 2021-06-09 F. Hoffmann-La Roche AG Barcoded circular library construction for identification of chimeric products
IL319189A (en) * 2016-12-29 2025-04-01 Seegene Inc Method for reducing primer dimer formation and increasing amplification efficiency
JP7116062B2 (ja) * 2017-01-10 2022-08-09 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重化シグナル増幅
CN106868111B (zh) * 2017-01-13 2020-12-25 中玉金标记（北京）生物技术股份有限公司 利用通用TaqMan探针检测SNP的方法和试剂盒
CA3058202A1 (en) * 2017-04-17 2018-10-25 Montana State University Switch-like isothermal dna amplification demonstrating a non-linear amplification rate
WO2019033065A1 (en) * 2017-08-11 2019-02-14 Atila Biosystems, Inc. DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
KR102180462B1 (ko) * 2018-01-31 2020-11-18 한국과학기술원 삼중접합구조 기반 등온 핵산 증폭 기술을 이용한 표적핵산 검출 방법
JP7013960B2 (ja) * 2018-03-13 2022-02-01 株式会社リコー 核酸検出方法及び核酸検出装置、並びに、核酸検出キット
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
DE102018213026A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Verfahren zur Durchführung einer Echtzeit-PCR
GB2587178B (en) * 2019-01-09 2021-10-06 3Cr Bioscience Ltd Method
US20220090194A1 (en) * 2019-01-09 2022-03-24 3Cr Bioscience Ltd. Method for detecting a nucleic acid sequence
CN113454238B (zh) * 2019-01-25 2025-02-11 哈佛大学校长及研究员协会 用于合成核酸的组合物和方法
CN111593122A (zh) * 2019-02-20 2020-08-28 北京福安华生物科技有限公司 检测XPC基因rs2228001位点多态性的人工模拟核酸分子信标与试剂盒
CN111593105A (zh) * 2019-02-21 2020-08-28 葛猛 检测SOD2基因rs4880位点多态性的人工模拟核酸分子信标与试剂盒
CN110079584B (zh) * 2019-03-29 2020-07-28 中国农业大学 基于3d-hcr水凝胶的汞离子快速可视化检测方法
GB201910635D0 (en) * 2019-07-25 2019-09-11 Ttp Plc Detection of target oligonucleotides
CN112301103B (zh) * 2019-08-02 2023-04-11 北京贝瑞和康生物技术有限公司 一种非特异性扩增天然短片段核酸的方法和试剂盒
CN110542674B (zh) * 2019-09-19 2021-10-22 济南大学 一种检测谷胱甘肽的生物传感器及其制备方法
US20220356514A1 (en) * 2019-10-16 2022-11-10 Illumina, Inc. Systems and methods for detecting multiple analytes
US20230272462A1 (en) * 2020-07-09 2023-08-31 University Of Washington Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid
CN112899357A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种运用数字pcr进行胎儿分数检测的方法及其试剂盒
CN112899346A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用
CN112899365A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种肺癌基因突变位点的检测方法及其试剂盒
CN112899358A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种无创产前胎儿染色体非整倍体的检测方法及其试剂盒
CN112899366A (zh) * 2021-01-06 2021-06-04 南京普济生物有限公司 一种结直肠癌基因突变位点的检测方法及其试剂盒
CN112735527B (zh) * 2021-01-06 2022-09-13 武汉华大基因技术服务有限公司 一种串联序列解析方法、装置和存储介质
CN114958988A (zh) * 2021-02-27 2022-08-30 上海境象生物科技有限公司 基于探针熔解曲线分析的多重核酸检测方法和试剂盒
EP4320278A4 (en) * 2021-04-08 2025-05-07 Board of Regents, The University of Texas System METHODS AND SYSTEMS FOR HPV DETECTION AND QUANTIFICATION
KR20220142832A (ko) * 2021-04-15 2022-10-24 프로지니어 주식회사 G-사중체화 서열 기반의 표적 핵산 검출용 핵산 템플릿 및 이의 용도
EP4409039A4 (en) * 2021-09-29 2025-09-03 Pillar Biosciences Inc CUSTOMIZED LIQUID BIOPSIES FOR CANCER USING PRIMERS FROM A PRIMER BANK
WO2023067110A1 (en) * 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
CN114196740A (zh) * 2021-12-09 2022-03-18 南京普济生物有限公司 用于同时识别多种基因类型的数字扩增检测方法、检测产品和检测试剂盒
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
US20250297318A1 (en) * 2024-03-25 2025-09-25 Milaboratories Inc. Dna-based adaptome profiling for minimal residual disease quantification in lymphoid malignancies
CN119023958B (zh) * 2024-10-28 2025-05-06 宁波大学 基于多价适配体和cha扩增的比色-荧光双信号检测沙门氏菌的方法

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5418149A (en) 1990-07-24 1995-05-23 Hoffmann-La Roche Inc. Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
WO1989001050A1 (en) 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
DK0515506T3 (da) 1990-02-16 2000-05-08 Hoffmann La Roche Fremgangsmåde til påvisning af carcinogene humane papillomavirus
IL100040A (en) 1990-11-13 1995-12-31 Siska Diagnostics Inc Nucleic acid amplification by two enzyme self-sustained sequence replication
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP2000514307A (ja) 1996-07-16 2000-10-31 ジェン―プローブ・インコーポレーテッド 増加した標的特異的ｔ▲下ｍ▼を持つ修飾オリゴヌクレオチドを用いた核酸配列の検出および増幅のための方法
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
EP0866071B1 (en) 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
AR021833A1 (es) * 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
WO2002014555A2 (en) 2000-08-11 2002-02-21 University Of Utah Research Foundation Single-labeled oligonucleotide probes
WO2003008624A2 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Nucleic acid amplification using nicking agents
DE10236406C1 (de) * 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
EP1604040B1 (en) * 2003-03-07 2010-10-13 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US7387876B2 (en) * 2004-02-27 2008-06-17 President And Fellows Of Harvard College Amplification of trace amounts of nucleic acids
WO2005098049A2 (en) 2004-03-25 2005-10-20 California Institute Of Technology Hybridization chain reaction
US8871469B1 (en) 2004-11-13 2014-10-28 Steven Albert Benner Self-avoiding molecular recognition systems in DNA priming
US7833942B2 (en) * 2004-12-03 2010-11-16 Human Genetic Signatures Pty. Ltd. Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US20070212704A1 (en) * 2005-10-03 2007-09-13 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US20090036315A1 (en) * 2006-02-07 2009-02-05 Antara Biosciences Inc. Device and methods for detecting and quantifying one or more target agents
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
KR101110013B1 (ko) * 2007-10-05 2012-02-29 (주)바이오니아 서열 내에 어베이직 부분을 포함하는 ｐｃｒ 증폭용프라이머
KR20100019220A (ko) * 2008-08-08 2010-02-18 삼성전자주식회사 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법
US8658366B2 (en) 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
EP3460074B1 (en) * 2010-02-05 2020-10-07 Quest Diagnostics Investments Incorporated Method to detect repeat sequence motifs in nucleic acid
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US20140065613A1 (en) 2012-09-06 2014-03-06 Life Technologies Corporation Multiplex Y-STR Analysis
US20140274811A1 (en) * 2013-03-14 2014-09-18 Lyle J. Arnold Methods for Amplifying a Complete Genome or Transcriptome
HRP20210953T1 (hr) * 2014-02-18 2021-09-03 Illumina, Inc. Postupci i pripravci za određivanje dnk profila
CA2983819A1 (en) 2015-04-24 2016-10-27 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
WO2019033065A1 (en) 2017-08-11 2019-02-14 Atila Biosystems, Inc. DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS

Also Published As

Publication number Publication date
CN114250274A (zh) 2022-03-29
AU2022200725A1 (en) 2022-02-24
EP3286338B1 (en) 2023-11-01
JP2018514230A (ja) 2018-06-07
CA2983819A1 (en) 2016-10-27
US11091799B2 (en) 2021-08-17
AU2016252998B2 (en) 2021-11-04
JP2022008577A (ja) 2022-01-13
WO2016172632A3 (en) 2017-01-12
US20180148775A1 (en) 2018-05-31
US20220033891A1 (en) 2022-02-03
EP3286338C0 (en) 2023-11-01
ES2961374T3 (es) 2024-03-11
CN107849603A (zh) 2018-03-27
WO2016172632A2 (en) 2016-10-27
JP6966681B2 (ja) 2021-11-17
EP3286338A4 (en) 2018-12-19
EP3286338A2 (en) 2018-02-28
AU2016252998A1 (en) 2017-12-14

Similar Documents

Publication Publication Date Title
CN107849603B (zh) 利用有限核苷酸组成的引物扩增
JP5805064B2 (ja) 対立遺伝子変種を検出するための方法、組成物、およびキット
US8673567B2 (en) Method and kit for nucleic acid sequence detection
US8034568B2 (en) Isothermal nucleic acid amplification methods and compositions
EP2467479B1 (en) Compositions and methods for intramolecular nucleic acid rearrangement
AU2012304520B2 (en) Circularized templates for sequencing
CN107429292B (zh) 底数大于2的指数核酸扩增
WO2009102896A2 (en) Isothermal nucleic acid amplification methods and compositions
WO2011055232A2 (en) Base-by-base mutation screening
CN113227398A (zh) 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增
RU2729983C2 (ru) Способ амплификации днк на основе внедрения в цепь
CN110468179A (zh) 选择性扩增核酸序列的方法
CN104955962B (zh) 核酸扩增方法
EP4479556B1 (en) Internal controls for nucleic acid amplification
HK40064470A (en) Amplification with primers of limited nucleotide composition
JP5914346B2 (ja) 非メチル化核酸の選択的蓄積
US9074248B1 (en) Primers for helicase dependent amplification and their methods of use
JP7628085B2 (ja) 鎖分離温度に基づくＤＮＡに対してＲＮＡ標的の優先的／選択的増幅のためのｄＩＴＰの利用
WO2018009677A1 (en) Fast target enrichment by multiplexed relay pcr with modified bubble primers
WO2013124743A1 (en) Compositions and methods for intramolecular nucleic acid rearrangement ii

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant