CN107736184A - A kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn - Google Patents
A kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn Download PDFInfo
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Abstract
The invention discloses a kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, pleurotus eryngii slant strains are wherein inoculated in plating medium activation, cultivate and select mycelia as parent species, stand to mycelia block and sprout, go to that shaking table is incubated to obtain liquid strain, liquid strain expands culture through shake-flask seed culture medium and seed tank culture base successively and obtains seeding tank seed step by step；By fermentation medium liquid amount and pour into fermentation tank, Water spray cools down and is passed through filtrated air after sterilizing, seeding tank seed is inoculated in fermentation tank, slow cooling after ferment at constant temperature, enter seeding tank seed to fermentation tank relaying continued access, cool after fermentation, then ferment at constant temperature can obtain high activity pleurotus eryngii liquid strain high activity pleurotus eryngii liquid strain；The present invention includes expanding culture strain, fermentation, charging, inoculation, culture, fruiting and harvesting packaging step by step, increases substantially the economic benefit that labor productivity, Qualified Products Rate further improve planting almond abalone mushroom.
Description
Technical field
The invention belongs to the technical field of field of edible fungus culture, more particularly to Pleurotus eryngii industrial production, and in particular to
A kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn.
Background technology
Pleurotus eryngii is that a kind of precious medicine eats all suitable fungi, and pleurotus eryngii bacterial context is plump, quality is tender and crisp, excellent taste, is
One of 16 kinds of edible Rare edible fungus are recommended by the World Health Organization and FAO (Food and Agriculture Organization of the United Nation).
Pleurotus eryngii industrial cultivation is a kind of new cultivation technique developed rapidly in the world at present, and Pleurotus eryngii industrial is given birth to
Produce is relative to the main distinction of the seasonal production of traditional greenhouse：First, efficiency high：From the configuration for being built into equipment of mushroom house,
Substantially design is all pressed, using mechanized operation, part uses Automated condtrol；Efficiency is substantially increased, is subtracted
Labor intensity is lacked；2nd, can whole year production：Factorial praluction is using the temperature needed for manual control Growth of Pleurotus eryngii, humidity, light
According to making it not influenceed by extraneous seasonal variations；3rd, land utilization ratio is big：Factorial praluction uses stereo planting, carries significantly
The high utilization rate of unit area；Fast development in China only has short several years, although updating in recent years,
There are still production cycle length, the problems such as link hand-manipulated is more, and benefit is not good enough.
Domestic and international planting almond abalone mushroom is based on solid spawn at present, and liquid spawn has production week relative to solid spawn
Phase is short, cell age is consistent, fruiting is neat, is easy to the advantages that management, strain cost are low, inoculation is convenient and quick, is advantageous to edible mushroom life
Scale, the batch production of production, in recent years due to the fast development of industrial cultivation technique, to shorten strain manufacturing cycle, improve
Strain quality and production efficiency, domestic and international manufacturer's research and development liquid spawn are used to replace in current pleurotus eryngii production generally
The solid spawn used, and start to apply in actual production.
The content of the invention
The technical problems to be solved by the invention are the shortcomings that presence for above prior art, are proposed a kind of based on height
The Pleurotus eryngii industrial cultivation method of active liquid strain, the cultural method is simple and easy, can make production technical standard, production
Quality equalizes, and increases substantially labor productivity, Qualified Products Rate, reduces pollution, reduces cost, further improves
The economic benefit of planting almond abalone mushroom.
The present invention solve above technical problem technical scheme be：
A kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, comprises the following steps：
Step（1）：Pleurotus eryngii slant strains are trained through liquid seed culture medium, shake-flask seed successively after plating medium activates
Support base and seed tank culture base expands culture and obtains seeding tank seed step by step, seeding tank seed is accessed in nutrient solution and obtains liquid
In mother；
Step（2）：Zymotic fluid is poured into fermentation tank, sterilize 60-100nin at 120 DEG C, in sterilizing after spraying cooling and lead to
Filtrated air is crossed, is inoculated with into cultured liquid parent species, inoculum concentration 3-5%, fermentation tank culture temperature is 20-35 DEG C, ventilation pressure
Power is 0.5-1.0 MPas, ferment at constant temperature culture 1-2 days, is then cooled to 18-20 DEG C then with 0.5-0.8 DEG C/min speed
Accessing liquid parent species again into fermentation tank, inoculum concentration 8-10%, ferment 18-20h under the conditions of throughput is 5-7L/min,
25-28 DEG C finally is warming up to 0.2-0.4 DEG C/min speed, ferment at constant temperature 3-7 days under the conditions of throughput is 3-5L/min
It is stand-by to can obtain pleurotus eryngii liquid strain；
Step（3）：Mixed culture material is chosen, and adds water stirring to prewet, it is 64%- to control the mixed culture material water content after prewetting
66%, PH 6-7, and by mixed culture material with wide 1.5m, high 1m, length is 1.5-2m isosceles trapezoid windrow, then in windrow
Commercial wooden stick inserts out some vertical stomatas, leads directly to windrow bottom, finally covers straw mat by its spontaneous fermentation；
Step（4）：By step（3）Mixed culture material after middle fermentation carries out packing pack from 22cm × 45cm Polypropylene Bag
Cultivation package is obtained, is then sent on the bedstead in mushroom house, steam is passed through after high-temperature sterilization, by temperature control in mushroom house at 60-65 DEG C,
5-7h is kept, stopping is passed through steam, is then being punched on cultivation package and hollow plastic rod, and cap beyond the Great Wall are inserted into hole,
By refrigerating work procedure slow cooling to 25-30 DEG C；
Refrigerating work procedure is specially：First natural cooling, after temperature is dropped to no more than 40 DEG C, forced refrigeration is carried out using refrigeration machine,
It is cooled to 25-30 DEG C；
Step（5）：Liquid spawn is inoculated into by step using air purification pipeline（4）In cultivation package after middle cooling, inoculation
When pull out sticking plaster in insertion cultivation package, and prick the increase ventilation of several apertures with pin on cultivation package and promote mycelia to sprout as early as possible
Hair；
Step（6）：Cultivation package after inoculation is delivered into temperature as 25-30 DEG C, relative air humidity is in 75-80% culturing room,
Cultivate 10-15 days and keep the dark state of culturing room；
Step（7）：After mycelial growth completely cultivates bag, temperature is moved into as 20-22 DEG C, relative air humidity is 90%-95% fruiting
In room, and the cap on cultivation package is pulled out, carry out management of producing mushroom, daily ventilation 4-7 times, each 10-15min, in fruiting
First 6-7 days of management increase intensity of illumination 300-700lx diffused light in culturing room；
Step（8）：After mushroom flower bud grows, cultivation package two is opened wide, continues to keep step（7）Ventilation, to the ground of mushroom producing room
Face, wall water spray, holding humidity is 85-90%, when fructification cap color from depth to shallow, lower concave part has white grass-like thing, cap
Edge starts upper volume, when spore not yet largely distributes, i.e., up to ripe very likely, for Harvesting Date harvesting packaging, it is damp harvest one, two
After mushroom, then it is managed to whole and harvests by above-mentioned management method.
Step（9）：Mushroom room is carried out in time after harvesting thoroughly to clean, sterilized, ventilation is used again after 1-2 days.
The technical scheme that further limits of the present invention as：
In the foregoing Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, step（1）Slant strains after middle activation
It is through concrete operations during shake-flask seed culture medium：
By access strain shake-flask seed culture medium be put into 24-26 DEG C, rotating speed be 170-190 turn/min shaking tables in cultivate 6-8 days,
And it is 6 to control pH value.
In the foregoing Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, shake-flask seed culture medium and seeding tank
Culture medium is identical, according to the mass fraction including following components：Wheat berry：50-60 parts, wood chip：20-30 parts, rape straw：7-9 parts,
Corncob：10-15 parts, wheat bran：9-11 parts, brown sugar：1-3 parts, magnesium sulfate：0.1-0.3 parts, lime：1-3 parts, potassium dihydrogen phosphate：
0.3-0.5 parts；
The preparation method of the culture medium is specially：
（1）With clear water to wheat rinsed clean used, the impurity of floating and empty flat particle are removed；
（2）Wheat berry or boiling wheat berry are soaked at a temperature of 40 DEG C -50 DEG C, is made untill wheat berry absorbs water to centre without white core；
（3）Brown sugar, potassium dihydrogen phosphate and magnesium sulfate are dissolved with water, obtain nutrient solution, then by the wheat berry of water swelling, wood
Bits, rape straw, corncob, wheat bran, lime and nutrient solution are mixed evenly, and are modulated to moisture content 60%-65%, are cultivated
Base, culture medium is bottled or packed, is sterilized, cooling.
In the foregoing Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, zymotic fluid wraps by mass percentage
Include following components：Glucose：3-5 parts, wheat bran 1-2 parts, corn flour：2-4 parts, peptone：0.3-0.5 parts, KH2PO4：0.1-
0.3 part, MgSO4：0.1-0.2 parts, VB：1 part, CMC：0.2-0.4 parts.
In the foregoing Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, mixed culture material prewet before by quality
Number meter includes following components：Corn stalk powder：70-80 parts, pleurotus eryngii bacteria residue：60-70 parts, potato：20-30 parts, bagasse：
10-20 parts, wheat bran：10-19 parts, cotton seed hulls：20-25 parts, dregs of beans：10-13 parts, magnesium sulfate：1-3 parts, polypropylene glycerol：0.4-
0.7 part, vitamin B1:1 part；
The pretreatment to be carried out before use of pleurotus eryngii bacteria residue, it is specially：Pleurotus eryngii bacteria residue is removed the peel, is then comminuted into thin
Grain；
Its stirring operation of prewetting is specially：Mixed culture material is stirred using pre- wet mixer, time 10-15min, then
Interlocked using micro computer time controller, magnetic valve and pressure pump, control amount of water as needed, be stirred at least 15 minutes
Mixed culture material is made.
Technique effect, the major ingredient of current Xinbao mushroom culturing are wood chip or cotton seed hulls, expansion and wood with cultivated area
The reduction in aptitude source, sawdust resource is more and more nervous, has formd the contradiction of expansion Yu the resource reduction of cultivated area, cotton seed hulls
It is the byproduct in Cotton Production industry, cotton category agricultural economy crop, cotton planting amount is substantially stable, thus cotton seed hulls
Annual production is substantially also stable, and there is also crisis of resource, mixed culture medium of the present invention based on corn stalk, resource is come
Source is wide, cheap, and mycelia decomposes corn stalk filament, therefrom obtains carbon source as energy, and mixed culture material realizes
Pleurotus eryngii bacteria residue is beneficial to again, reduces environmental pollution caused by bacteria residue is stacked, and using our factory's waste material of edible mushroom, reduce
The high cost that other places allocation and transportation are brought.
Wheat berry and wood chip is used to expand numerous strain for the culture medium of primary raw material, resulting strain is nutritious, particle
Suitable size, it is energetic, sprout early, growth is fast, can in the substrate and stromal surface grows simultaneously, and the hair in cultivation matrix
Bacterium points are more, possess the condition of spice inoculation；
In summary, the mixed culture material formula used in the present invention is to be directed to nutrition goods and materials needed for pleurotus eryngii different phase,
Raw material of the proportioning containing nutrition needed for each stage, reach nutrition equilibrium, and the nutriment in the mixed culture material is easy to apricot
What abalone mushroom absorbed, so as to nutrition needed for fully improving each growth period of pleurotus eryngii, the speed of each growth period of pleurotus eryngii is speeded,
Whole growth cycle is set significantly to shorten；
Mixed culture material, which is stirred, using pre- wet mixer in the present invention makes the stirring of compost each component evenly, while profit
Interlocked with micro computer time controller, magnetic valve and pressure pump, control amount of water to make the humidity of each compost as needed
It is attained by basically identical, the humidity required by reaching；Bubble is invaded relative to traditional millpond using the spice of this mechanization
The labor intensity of worker is greatly reduced for raw material, improves operating efficiency, makes the whole technological process of production, production technology
Standardization, product quality equalization.
In the foregoing Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, step（7）Middle cultivation package is in fruiting
Carry out increasing diffused light, daily illumination 0-9h during management of producing mushroom in room.
In the foregoing Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, step（7）Middle cultivation package is in fruiting
Carry out increasing diffused light, daily illumination 24h during management of producing mushroom in room.
The beneficial effects of the invention are as follows：
Industrial planting method in the present invention utilizes modernization project technology and sophisticated equipment, is manually set, automatically controls apricot
Temperature that abalone mushroom grows, humidity, illumination, etc. environment, make the technological process of production, production technical standard, product quality
Equalization, in the pleurotus eryngii production anniversary, labor productivity, Qualified Products Rate and biological conversion rate are increased substantially, is subtracted
Of low pollution, cost being reduced, further improve the economic benefit of planting almond abalone mushroom so that pleurotus eryngii steady quality, product quality is high,
Do not influenceed by natural conditions, the simple production process, be adapted to large-scale industrial production, substantially increase yield.
Embodiment
Embodiment 1
The present embodiment provides a kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, comprises the following steps：
Step（1）：Pleurotus eryngii slant strains are trained through liquid seed culture medium, shake-flask seed successively after plating medium activates
Support base and seed tank culture base expands culture and obtains seeding tank seed step by step, seeding tank seed is accessed in nutrient solution and obtains liquid
In mother；
Shake-flask seed culture medium is identical with seed tank culture base, according to the mass fraction including following components：Wheat berry：50 parts, wood
Bits：20 parts, rape straw：7 parts, corncob：10 parts, wheat bran：9 parts, brown sugar：1 part, magnesium sulfate：0.1 part, lime：1 part, di(2-ethylhexyl)phosphate
Hydrogen potassium：0.3 part；
The preparation method of the culture medium is specially：
（1）With clear water to wheat rinsed clean used, the impurity of floating and empty flat particle are removed；
（2）Wheat berry or boiling wheat berry are soaked at a temperature of 40 DEG C DEG C, is made untill wheat berry absorbs water to centre without white core；
（3）Brown sugar, potassium dihydrogen phosphate and magnesium sulfate are dissolved with water, obtain nutrient solution, then by the wheat berry of water swelling, wood
Bits, rape straw, corncob, wheat bran, lime and nutrient solution are mixed evenly, and are modulated to moisture content 60%%, obtain culture medium,
Culture medium is bottled or packed, is sterilized, cooling；
Step（2）：Zymotic fluid is poured into fermentation tank, sterilize 60nin at 120 DEG C, in sterilizing after spraying cooling and pass through nothing
Bacterium air, it is inoculated with into cultured liquid parent species, inoculum concentration 3%, fermentation tank culture temperature is 20 DEG C, and venting pressure is 0.5 million
Pa, ferment at constant temperature culture 1 day, then it is cooled to 18 DEG C with 0.5 DEG C/min speed and then accesses liquid again into fermentation tank
Parent species, inoculum concentration 8%, ferment 18h under the conditions of throughput is 5L/min, is finally warming up to 25 with 0.2 DEG C/min speed
DEG C, it is stand-by to can obtain pleurotus eryngii liquid strain in 3 days for ferment at constant temperature under the conditions of throughput is 3L/min；
Zymotic fluid includes following components by mass percentage：Glucose：3 parts, 1 part of wheat bran, corn flour：2 parts, peptone：0.3
Part, KH2PO4：0.1 part, MgSO4：0.1 part, VB：1 part, CMC：0.2 part；
Step（3）：Mixed culture material is chosen, and adds water stirring to prewet, it is 64% to control the mixed culture material water content after prewetting,
PH is 6, and by mixed culture material with wide 1.5m, high 1m, length is 1.5m isosceles trapezoid windrow, then in windrow commercialization wooden stick
Some vertical stomatas are inserted out, windrow bottom is led directly to, finally covers straw mat by its spontaneous fermentation；
Mixed culture material includes following components according to the mass fraction before prewetting：Corn stalk powder：70 parts, pleurotus eryngii bacteria residue：60 parts, soil
Beans：20 parts, bagasse：10 parts, wheat bran：10-19 parts, cotton seed hulls：20 parts, dregs of beans：10 parts, magnesium sulfate：1 part, polypropylene glycerol：
0.4 part, vitamin B1:1 part；
The pretreatment to be carried out before use of pleurotus eryngii bacteria residue, it is specially：Pleurotus eryngii bacteria residue is removed the peel, is then comminuted into thin
Grain；
Stirring operation of prewetting is specially：Mixed culture material is stirred using pre- wet mixer, time 10min, recycled
Micro computer time controller, magnetic valve and pressure pump interlock, and control amount of water as needed, are stirred for being made at least 15 minutes
Mixed culture material；
Step（4）：By step（3）Mixed culture material after middle fermentation carries out packing pack from 22cm × 45cm Polypropylene Bag
Cultivation package is obtained, is then sent on the bedstead in mushroom house, steam is passed through after high-temperature sterilization, by temperature control in mushroom house at 60 DEG C, is protected
5h is held, stopping is passed through steam, then inserts hollow plastic rod, and cap beyond the Great Wall in the punching on cultivation package and into hole, by
Refrigerating work procedure slow cooling is to 25 DEG C；
Refrigerating work procedure is specially：First natural cooling, after temperature is dropped to no more than 40 DEG C, forced refrigeration is carried out using refrigeration machine,
It is cooled to 25 DEG C；
Step（5）：Liquid spawn is inoculated into by step using air purification pipeline（4）In cultivation package after middle cooling, inoculation
When pull out sticking plaster in insertion cultivation package, and prick several apertures with pin on cultivation package；
Step（6）：Cultivation package after inoculation is delivered into temperature as 25 DEG C, relative air humidity is culture in 75% culturing room
10-15 days and keep the dark state of culturing room；
Step（7）：After mycelial growth completely cultivates bag, temperature is moved into as 20 DEG C, relative air humidity is in 90% mushroom producing room, and
Pull out the cap on cultivation package, carry out management of producing mushroom, daily ventilation 4 times, each 10min；
Step（8）：After mushroom flower bud grows, cultivation package two is opened wide, continues to keep step（7）Ventilation, to the ground of mushroom producing room
Face, wall water spray, keep humidity be 85, when fructification cap color from depth to shallow, lower concave part has white grass-like thing, cap edge
Start upper volume, when spore not yet largely distributes, i.e., up to ripe very likely, for Harvesting Date harvesting packaging, harvest one, two damp mushrooms
Afterwards, then it is managed to whole and harvests by above-mentioned management method.
Step（9）：Mushroom room is carried out in time after harvesting thoroughly to clean, sterilized, ventilation is used again after 1 day.
In the present embodiment：Step（1）Slant strains after middle activation are through concrete operations during shake-flask seed culture medium：
By the shake-flask seed culture medium for accessing strain be put into 24 DEG C, rotating speed be to be cultivated 6 days in 170 turns/min shaking tables, and control PH
It is worth for 6.
Embodiment 2
The present embodiment provides a kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, comprises the following steps：
Step（1）：Pleurotus eryngii slant strains are trained through liquid seed culture medium, shake-flask seed successively after plating medium activates
Support base and seed tank culture base expands culture and obtains seeding tank seed step by step, seeding tank seed is accessed in nutrient solution and obtains liquid
In mother；
Shake-flask seed culture medium is identical with seed tank culture base, according to the mass fraction including following components：Wheat berry：60 parts, wood
Bits：30 parts, rape straw：9 parts, corncob：15 parts, wheat bran：11 parts, brown sugar：3 parts, magnesium sulfate：0.3 part, lime：3 parts, phosphoric acid
Potassium dihydrogen：0.5 part；
The preparation method of the culture medium is specially：
（1）With clear water to wheat rinsed clean used, the impurity of floating and empty flat particle are removed；
（2）Wheat berry or boiling wheat berry are soaked at a temperature of 50 DEG C, is made untill wheat berry absorbs water to centre without white core；
（3）Brown sugar, potassium dihydrogen phosphate and magnesium sulfate are dissolved with water, obtain nutrient solution, then by the wheat berry of water swelling, wood
Bits, rape straw, corncob, wheat bran, lime and nutrient solution are mixed evenly, and are modulated to moisture content 65%, obtain culture medium, will
Culture medium is bottled or pack, sterilizing, cooling；
Step（2）：Zymotic fluid is poured into fermentation tank, sterilize 100nin at 120 DEG C, in sterilizing after spraying cooling and pass through nothing
Bacterium air, it is inoculated with into cultured liquid parent species, inoculum concentration 5%, fermentation tank culture temperature is 35 DEG C, and venting pressure is 1.0 million
Pa, ferment at constant temperature culture 2 days, then it is cooled to 20 DEG C with 0.8 DEG C/min speed and then accesses liquid again into fermentation tank
Parent species, inoculum concentration 10%, ferment 20h under the conditions of throughput is 5-7L/min, is finally warming up to 0.4 DEG C/min speed
28 DEG C, it is stand-by to can obtain pleurotus eryngii liquid strain in 7 days for ferment at constant temperature under the conditions of throughput is 5L/min；
Zymotic fluid includes following components by mass percentage：Glucose：5 parts, wheat bran 1-2 parts, corn flour：2-4 parts, albumen
Peptone：0.5 part, KH2PO4：0.3 part, MgSO4：0.2 part, VB：1 part, CMC：0.2-0.4 parts；
Step（3）：Mixed culture material is chosen, and adds water stirring to prewet, it is 66% to control the mixed culture material water content after prewetting,
PH is 7, and by mixed culture material with wide 1.5m, high 1m, length is 12m isosceles trapezoid windrow, then in windrow commercialization wooden stick
Some vertical stomatas are inserted out, windrow bottom is led directly to, finally covers straw mat by its spontaneous fermentation；
Mixed culture material includes following components according to the mass fraction before prewetting：Corn stalk powder：80 parts, pleurotus eryngii bacteria residue：70 parts, soil
Beans：30 parts, bagasse：20 parts, wheat bran：19 parts, cotton seed hulls：25 parts, dregs of beans：13 parts, magnesium sulfate：3 parts, polypropylene glycerol：0.7
Part, vitamin B1:1 part；
The pretreatment to be carried out before use of pleurotus eryngii bacteria residue, it is specially：Pleurotus eryngii bacteria residue is removed the peel, is then comminuted into thin
Grain；
Stirring operation of prewetting is specially：Mixed culture material is stirred using pre- wet mixer, time 15min, recycled
Micro computer time controller, magnetic valve and pressure pump interlock, and control amount of water as needed, are stirred for being made at least 15 minutes
Mixed culture material；
Step（4）：By step（3）Mixed culture material after middle fermentation carries out packing pack from 22cm × 45cm Polypropylene Bag
Cultivation package is obtained, is then sent on the bedstead in mushroom house, steam is passed through after high-temperature sterilization, by temperature control in mushroom house at 65 DEG C, is protected
7h is held, stopping is passed through steam, then inserts hollow plastic rod, and cap beyond the Great Wall in the punching on cultivation package and into hole, by
Refrigerating work procedure slow cooling is to 30 DEG C；
Refrigerating work procedure is specially：First natural cooling, after temperature is dropped to no more than 40 DEG C, forced refrigeration is carried out using refrigeration machine,
It is cooled to 30 DEG C；
Step（5）：Liquid spawn is inoculated into by step using air purification pipeline（4）In cultivation package after middle cooling, inoculation
When pull out sticking plaster in insertion cultivation package, and prick several apertures with pin on cultivation package；
Step（6）：Cultivation package after inoculation is delivered into temperature as 30 DEG C, relative air humidity is culture 15 in 80% culturing room
It simultaneously keeps the dark state of culturing room；
Step（7）：After mycelial growth completely cultivates bag, temperature is moved into as 22 DEG C, relative air humidity is in 95% mushroom producing room, and
Pull out the cap on cultivation package, carry out management of producing mushroom, daily ventilation 7 times, each 15min, first 7 days of management of producing mushroom
Increase intensity of illumination 700lx diffused light, daily illumination 9h in culturing room；
Step（8）：After mushroom flower bud grows, cultivation package two is opened wide, continues to keep step（7）Ventilation, to the ground of mushroom producing room
Face, wall water spray, keep humidity be 90%, when fructification cap color from depth to shallow, lower concave part has white grass-like thing, cap side
Edge starts upper volume, when spore not yet largely distributes, i.e., up to ripe very likely, for Harvesting Date harvesting packaging, has harvested one, two damp mushrooms
Afterwards, then it is managed to whole and harvests by above-mentioned management method.
Step（9）：Mushroom room is carried out in time after harvesting thoroughly to clean, sterilized, ventilation is used again after 2 days.
In the present embodiment：Step（1）Slant strains after middle activation are through concrete operations during shake-flask seed culture medium：
By the shake-flask seed culture medium for accessing strain be put into 26 DEG C, rotating speed be to be cultivated 8 days in 190 turns/min shaking tables, and control PH
It is worth for 6.
Embodiment 3
The present embodiment provides a kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, comprises the following steps：
Step（1）：Pleurotus eryngii slant strains are trained through liquid seed culture medium, shake-flask seed successively after plating medium activates
Support base and seed tank culture base expands culture and obtains seeding tank seed step by step, seeding tank seed is accessed in nutrient solution and obtains liquid
In mother；
Shake-flask seed culture medium is identical with seed tank culture base, according to the mass fraction including following components：Wheat berry：55 parts, wood
Bits：25 parts, rape straw：8 parts, corncob：13 parts, wheat bran：10 parts, brown sugar：2 parts, magnesium sulfate：0.2 part, lime：2 parts, phosphoric acid
Potassium dihydrogen：0.4 part；
The preparation method of the culture medium is specially：
（1）With clear water to wheat rinsed clean used, the impurity of floating and empty flat particle are removed；
（2）Wheat berry or boiling wheat berry are soaked at a temperature of 45 DEG C, is made untill wheat berry absorbs water to centre without white core；
（3）Brown sugar, potassium dihydrogen phosphate and magnesium sulfate are dissolved with water, obtain nutrient solution, then by the wheat berry of water swelling, wood
Bits, rape straw, corncob, wheat bran, lime and nutrient solution are mixed evenly, and are modulated to moisture content 63%, obtain culture medium, will
Culture medium is bottled or pack, sterilizing, cooling；
Step（2）：Zymotic fluid is poured into fermentation tank, sterilize 80nin at 120 DEG C, in sterilizing after spraying cooling and pass through nothing
Bacterium air, it is inoculated with into cultured liquid parent species, inoculum concentration 4%, fermentation tank culture temperature is 27 DEG C, and venting pressure is 0.8 million
Pa, ferment at constant temperature culture 1 day, then it is cooled to 19 DEG C with 0.6 DEG C/min speed and then accesses liquid again into fermentation tank
Parent species, inoculum concentration 9%, ferment 19h under the conditions of throughput is 6L/min, is finally warming up to 26 with 0.3 DEG C/min speed
DEG C, it is stand-by to can obtain pleurotus eryngii liquid strain in 5 days for ferment at constant temperature under the conditions of throughput is 4L/min；
Zymotic fluid includes following components by mass percentage：Glucose：4 parts, 2 parts of wheat bran, corn flour：3 parts, peptone：0.4
Part, KH2PO4：0.2 part, MgSO4：0.1 part, VB：1 part, CMC：0.3 part；
Step（3）：Mixed culture material is chosen, and adds water stirring to prewet, it is 65% to control the mixed culture material water content after prewetting,
PH is 7, and by mixed culture material with wide 1.5m, high 1m, length is 1.8m isosceles trapezoid windrow, then in windrow commercialization wooden stick
Some vertical stomatas are inserted out, windrow bottom is led directly to, finally covers straw mat by its spontaneous fermentation；
Mixed culture material includes following components according to the mass fraction before prewetting：Corn stalk powder：75 parts, pleurotus eryngii bacteria residue：65 parts, soil
Beans：25 parts, bagasse：15 parts, wheat bran：15 parts, cotton seed hulls：23 parts, dregs of beans：12 parts, magnesium sulfate：1-3 parts, polypropylene glycerol：
0.6 part, vitamin B1:1 part；
The pretreatment to be carried out before use of pleurotus eryngii bacteria residue, it is specially：Pleurotus eryngii bacteria residue is removed the peel, is then comminuted into thin
Grain；
Stirring operation of prewetting is specially：Mixed culture material is stirred using pre- wet mixer, time 13min, recycled
Micro computer time controller, magnetic valve and pressure pump interlock, and control amount of water as needed, are stirred for being made at least 15 minutes
Mixed culture material；
Step（4）：By step（3）Mixed culture material after middle fermentation carries out packing pack from 22cm × 45cm Polypropylene Bag
Cultivation package is obtained, is then sent on the bedstead in mushroom house, steam is passed through after high-temperature sterilization, by temperature control in mushroom house at 63 DEG C, is protected
6h is held, stopping is passed through steam, then inserts hollow plastic rod, and cap beyond the Great Wall in the punching on cultivation package and into hole, by
Refrigerating work procedure slow cooling is to 28 DEG C；
Refrigerating work procedure is specially：First natural cooling, after temperature is dropped to no more than 40 DEG C, forced refrigeration is carried out using refrigeration machine,
It is cooled to 8 DEG C；
Step（5）：Liquid spawn is inoculated into by step using air purification pipeline（4）In cultivation package after middle cooling, inoculation
When pull out sticking plaster in insertion cultivation package, and prick several apertures with pin on cultivation package；
Step（6）：Cultivation package after inoculation is delivered into temperature as 28 DEG C, relative air humidity is culture 13 in 78% culturing room
It simultaneously keeps the dark state of culturing room；
Step（7）：After mycelial growth completely cultivates bag, temperature is moved into as 21 DEG C, relative air humidity is in 93% mushroom producing room, and
Pull out the cap on cultivation package, carry out management of producing mushroom, daily ventilation 5 times, each 13min, first 7 days of management of producing mushroom
Increase intensity of illumination 500lx diffused light, daily illumination 24h in culturing room；
Step（8）：After mushroom flower bud grows, cultivation package two is opened wide, continues to keep step（7）Ventilation, to the ground of mushroom producing room
Face, wall water spray, keep humidity be 88%, when fructification cap color from depth to shallow, lower concave part has white grass-like thing, cap side
Edge starts upper volume, when spore not yet largely distributes, i.e., up to ripe very likely, for Harvesting Date harvesting packaging, has harvested one, two damp mushrooms
Afterwards, then it is managed to whole and harvests by above-mentioned management method.
Step（9）：Mushroom room is carried out in time after harvesting thoroughly to clean, sterilized, ventilation is used again after 2 days.
In the present embodiment：Step（1）Slant strains after middle activation are through concrete operations during shake-flask seed culture medium：
By the shake-flask seed culture medium for accessing strain be put into 25 DEG C, rotating speed be to be cultivated 7 days in 180 turns/min shaking tables, and control PH
It is worth for 6.
In addition to the implementation, the present invention can also have other embodiment.It is all to use equivalent substitution or equivalent transformation shape
Into technical scheme, all fall within the protection domains of application claims.
Claims (7)
1. a kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn, it is characterised in that comprise the following steps：
Step（1）：Pleurotus eryngii slant strains are trained through liquid seed culture medium, shake-flask seed successively after plating medium activates
Support base and seed tank culture base expands culture and obtains seeding tank seed step by step, seeding tank seed is accessed in nutrient solution and obtains liquid
In mother；
Step（2）：Zymotic fluid is poured into fermentation tank, sterilize 60-100nin at 120 DEG C, in sterilizing after spraying cooling and lead to
Filtrated air is crossed, is inoculated with into cultured liquid parent species, inoculum concentration 3-5%, fermentation tank culture temperature is 20-35 DEG C, ventilation pressure
Power is 0.5-1.0 MPas, ferment at constant temperature culture 1-2 days, is then cooled to 18-20 DEG C then with 0.5-0.8 DEG C/min speed
Accessing liquid parent species again into fermentation tank, inoculum concentration 8-10%, ferment 18-20h under the conditions of throughput is 5-7L/min,
25-28 DEG C finally is warming up to 0.2-0.4 DEG C/min speed, ferment at constant temperature 3-7 days under the conditions of throughput is 3-5L/min
It is stand-by to can obtain pleurotus eryngii liquid strain；
Step（3）：Mixed culture material is chosen, and adds water stirring to prewet, it is 64%- to control the mixed culture material water content after prewetting
66%, PH 6-7, and by mixed culture material with wide 1.5m, high 1m, length is 1.5-2m isosceles trapezoid windrow, then in windrow
Commercial wooden stick inserts out some vertical stomatas, leads directly to windrow bottom, finally covers straw mat by its spontaneous fermentation；
Step（4）：By step（3）Mixed culture material after middle fermentation carries out packing pack from 22cm × 45cm Polypropylene Bag
Cultivation package is obtained, is then sent on the bedstead in mushroom house, steam is passed through after high-temperature sterilization, by temperature control in mushroom house at 60-65 DEG C,
5-7h is kept, stopping is passed through steam, is then being punched on cultivation package and hollow plastic rod, and cap beyond the Great Wall are inserted into hole,
By refrigerating work procedure slow cooling to 25-30 DEG C；
Described refrigerating work procedure is specially：First natural cooling, after temperature is dropped to no more than 40 DEG C, forced using refrigeration machine
Refrigeration, is cooled to 25-30 DEG C；
Step（5）：Liquid spawn is inoculated into by step using air purification pipeline（4）In cultivation package after middle cooling, inoculation
When pull out sticking plaster in insertion cultivation package, and prick several apertures with pin on cultivation package；
Step（6）：Cultivation package after inoculation is delivered into temperature as 25-30 DEG C, relative air humidity is in 75-80% culturing room,
Cultivate 10-15 days and keep the dark state of culturing room；
Step（7）：After mycelial growth completely cultivates bag, temperature is moved into as 20-22 DEG C, relative air humidity is 90%-95% fruiting
In room, and the cap on cultivation package is pulled out, carry out management of producing mushroom, daily ventilation 4-7 times, each 10-15min, in fruiting
First 6-7 days of management increase intensity of illumination 300-700lx diffused light in culturing room；
Step（8）：After mushroom flower bud grows, cultivation package two is opened wide, continues to keep step（7）Ventilation, to the ground of mushroom producing room
Face, wall water spray, holding humidity is 85-90%, when fructification cap color from depth to shallow, lower concave part has white grass-like thing, cap
Edge starts upper volume, when spore not yet largely distributes, i.e., up to ripe very likely, for Harvesting Date harvesting packaging, it is damp harvest one, two
After mushroom, then it is managed to whole and harvests by above-mentioned management method；
Step（9）：Mushroom room is carried out in time after harvesting thoroughly to clean, sterilized, ventilation is used again after 1-2 days.
2. the Pleurotus eryngii industrial cultivation method according to claim 1 based on high activity liquid spawn, it is characterised in that：
Step（1）Slant strains after middle activation are through concrete operations during shake-flask seed culture medium：
By access strain shake-flask seed culture medium be put into 24-26 DEG C, rotating speed be 170-190 turn/min shaking tables in cultivate 6-8 days,
And it is 6 to control pH value.
3. the Pleurotus eryngii industrial cultivation method according to claim 1 based on high activity liquid spawn, it is characterised in that：
Described shake-flask seed culture medium is identical with seed tank culture base, according to the mass fraction including following components：Wheat berry：50-60
Part, wood chip：20-30 parts, rape straw：7-9 parts, corncob：10-15 parts, wheat bran：9-11 parts, brown sugar：1-3 parts, magnesium sulfate：
0.1-0.3 parts, lime：1-3 parts, potassium dihydrogen phosphate：0.3-0.5 parts；
The preparation method of the culture medium is specially：
（1）With clear water to wheat rinsed clean used, the impurity of floating and empty flat particle are removed；
（2）Wheat berry or boiling wheat berry are soaked at a temperature of 40 DEG C -50 DEG C, is made untill wheat berry absorbs water to centre without white core；
（3）Brown sugar, potassium dihydrogen phosphate and magnesium sulfate are dissolved with water, obtain nutrient solution, then by the wheat berry of water swelling, wood
Bits, rape straw, corncob, wheat bran, lime and nutrient solution are mixed evenly, and are modulated to moisture content 60%-65%, are cultivated
Base, culture medium is bottled or packed, is sterilized, cooling.
4. the Pleurotus eryngii industrial cultivation method according to claim 1 based on high activity liquid spawn, it is characterised in that：
Described zymotic fluid includes following components by mass percentage：Glucose：3-5 parts, wheat bran 1-2 parts, corn flour：2-4 parts, egg
White peptone：0.3-0.5 parts, KH2PO4：0.1-0.3 parts, MgSO4：0.1-0.2 parts, VB：1 part, CMC：0.2-0.4 parts.
5. the Pleurotus eryngii industrial cultivation method according to claim 1 based on high activity liquid spawn, it is characterised in that：
The mixed culture material includes following components according to the mass fraction before prewetting：Corn stalk powder：70-80 parts, pleurotus eryngii bacteria residue：60-
70 parts, potato：20-30 parts, bagasse：10-20 parts, wheat bran：10-19 parts, cotton seed hulls：20-25 parts, dregs of beans：10-13 parts, sulphur
Sour magnesium：1-3 parts, polypropylene glycerol：0.4-0.7 parts, vitamin B1:1 part；
Pleurotus eryngii bacteria residue pretreatment to be carried out before use, it is specially：Pleurotus eryngii bacteria residue is removed the peel, is then comminuted into thin
Particle；
Stirring operation of prewetting described in it is specially：Mixed culture material is stirred using pre- wet mixer, time 10-
15min, recycle micro computer time controller, magnetic valve and pressure pump to interlock, control amount of water as needed, be stirred for
Few 15 minutes obtained mixed culture materials.
6. the Pleurotus eryngii industrial cultivation method according to claim 1 based on high activity liquid spawn, it is characterised in that：
Step（7）Middle cultivation package carries out increasing diffused light, daily illumination 0-9h during management of producing mushroom in mushroom producing room.
7. the Pleurotus eryngii industrial cultivation method according to claim 1 based on high activity liquid spawn, it is characterised in that：
Step（7）Middle cultivation package carries out increasing diffused light, daily illumination 24h during management of producing mushroom in mushroom producing room.
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| CN108834748A (en) * | 2018-07-25 | 2018-11-20 | 蓬溪县琪英菌业有限公司 | The production method of Pleurotus eryngii |
| CN108834747A (en) * | 2018-07-25 | 2018-11-20 | 蓬溪县琪英菌业有限公司 | A method of promoting pleurotus eryngii fruiting uniformity |
| CN109287374A (en) * | 2018-08-31 | 2019-02-01 | 湖州蝉知堂生物科技有限公司 | A kind of method of the two-sided culture of entomogenous fungi |
| CN110352797A (en) * | 2019-08-26 | 2019-10-22 | 贵州贵旺生物科技有限公司 | A kind of fermentation process of high activity pleurotus eryngii liquid strain |
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| IT201900024123A1 (en) | 2019-12-16 | 2021-06-16 | Giovanni Pacioni | PROCEDURE FOR THE SYNCHRONOUS AND PROGRAMMED PRODUCTION OF PLEUROTUS ERYNGII |
| CN113079951A (en) * | 2021-05-24 | 2021-07-09 | 宁德师范学院 | Preparation method of wide-substrate-adaptability edible fungus liquid strain |
| CN113973646A (en) * | 2021-07-28 | 2022-01-28 | 盐城工学院 | Culture medium and culture method for improving yield of pleurotus eryngii |
| CN114402913A (en) * | 2022-03-11 | 2022-04-29 | 南京吾悦农业科技有限公司 | Pleurotus eryngii culture medium and preparation method of original mother seeds |
| CN115005010A (en) * | 2022-05-11 | 2022-09-06 | 黑龙江省冰榕生物科技有限公司 | Pleurotus eryngii cultivation method based on liquid strains |
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| CN115005010A (en) * | 2022-05-11 | 2022-09-06 | 黑龙江省冰榕生物科技有限公司 | Pleurotus eryngii cultivation method based on liquid strains |
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