CN107494141A - A kind of breeding method of iceberg Chinese rose dwarfted varieties - Google Patents
A kind of breeding method of iceberg Chinese rose dwarfted varieties Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
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Abstract
The invention discloses a kind of breeding method of iceberg Chinese rose dwarfted varieties, it is characterised in that including following aspect：（1）Presprouting of seeds, first seed is immersed respectively in liquor potassic permanganate and auxin solution and soaked, be then immersed in progress constant temperature vernalization in functional liquid；（2）Nursery is downgraded, chitting piece is sowed into seedling bed and carries out nursery, and adjusting ambient pressure and gas concentration lwevel are cultivated；（3）Nursery is induced, first seedling is sprayed using auxin solution, then carries out magnetization induction；（4）Post-processing, half ring cutting is carried out when seedling is grown to 14 17cm, and parcel culture is carried out to plant using cotton, periodically sprinkle functional liquid.
Description
Technical field
The invention belongs to Chinese rose planting technology field, and in particular to a kind of breeding method of iceberg Chinese rose dwarfted varieties.
Background technology
China rose, also known as Chinese rose, it is described as spending middle queen, is evergreen or half green shrub flower variety, its is beautiful in colour,
It is various in style, there are the kinds such as red, powder is yellow, white, colour mixture, silver-colored side, kind reaches as many as up to ten thousand kinds, and Chinese modern rose cultivarses there are thousand kinds
More than.China rose honor is beautiful, good looks are various, four when normally opened, pleasant aroma, it is and strong adaptability, cold-resistant, drought-enduring, extensively should
It can be used for extraction essence and antifungic action for beautifying the yard, decorateeing field, the flowers such as gardens, arrangement flower bed, have higher
Ornamental values and the economic values, liked by various countries, have been widely cited plantation, it is located at the 5th, China's " ten big famous flowers ".
Iceberg Chinese rose is spent as pure white entirely, and the Rosa floribunda for have fragrance, spending more, frequently opening, is the treasure in modern rose cultivarses.But iceberg
Chinese rose plant strain growth height is high, is not suitable for as household balcony pot variety；Potted plant middle limb growth is too high, it is necessary to consume a large amount of
Nutrient, be unfavorable for Cultivate administration in large-scale production, occur that the time at florescence is short using method for potted, the feelings such as quantity of blooming is few
Condition；And pruning method is used, cross growth can only be limited, it is impossible to effectively limit longitudinal growth, and former plant is caused necessarily
Infringement.
The content of the invention
The present invention is directed to the problem of existing：Iceberg Chinese rose plant strain growth height is high, is not suitable for as the potted plant product of household balcony
Kind；Potted plant middle limb grows too high, it is necessary to consume substantial amounts of nutrient, is unfavorable for Cultivate administration in large-scale production, use is potted plant
Situations such as method occurs that the time at florescence is short, and quantity of blooming is few；And pruning method is used, cross growth can only be limited, it is impossible to have
Effect limitation longitudinal growth, and former plant is caused necessarily to damage.To solve the above problems, the invention provides a kind of iceberg moon
Season dwarfted varieties breeding method.
The present invention is achieved by the following technical solutions：
A kind of breeding method of iceberg Chinese rose dwarfted varieties, comprises the following steps：
（1）Presprouting of seeds：First iceberg Chinese rose seed is immersed in liquor potassic permanganate and soaks 2-3min, detoxification treatment is carried out, subtracts
Low pest and disease damage rate, then immerse in auxin solution and soak 1-2h, seed cell activity and differentiation capability are improved, then again by seed
Immerse and constant temperature vernalization is carried out in functional liquid, containing compositions such as multivitamin, plant acid and alkaloids in functional liquid, kind can be improved
Daughter cell metabolism, promote germination, and disease and insect resistance and resistance ability, temperature can be improved after neutron absorption
For 18-23 DEG C, time 20-22h, chitting piece is made；
（2）Downgrade nursery：Chitting piece is sowed into seedling bed and carries out nursery, 22-25 DEG C of temperature, humidity 60%-65%, seed
Sprouting stage, pressure 0.5-0.7Mpa, carbon dioxide volumetric concentration are 0.06%-0.08%, spray 1 inhibitor, seed daily
Germinating growth stage, pressure 0.8-1.0Mpa, carbon dioxide volumetric concentration are 0.1%-0.12%, spray 1 inhibitor within every 2 days；
Growth of seedling external drag can be improved by providing hyperbaric environment, promote seed root and stem's diameter increment, and high carbon dioxide is dense
The composition such as absorption, its tacrolimus contained, aspirin and endoxan that spending can improve to inhibitor can be to the life of seedling
Length plays restriction effect；
（3）Induce nursery：First seedling is sprayed using auxin solution, then carries out magnetization induction, respectively frequency of use
For 0.3-0.4MHz and 0.5-0.6MHz, every 3 magnetization treatment 1 time, each 10-15min, the frequency checker frequency is 1 time/
5min, it is carried out continuously 10-12 days, obtains mutagenesis seedling；Its caused magnetic field intensity constantly changes, and increases feelings in growth of seedling resistance
Seedling can be stimulated to produce the tree characteristics for adapting to environmental change under condition, reduce the growing height of plant；
（4）Post-processing：When seedling is grown to 14-17cm, half ring cutting of every 0.5-1cm distances 1 time, can limit root to top
Nutrition supply, so as to limit plant height growth, every plant of ring cutting 3-4 knife, and parcel culture is carried out to plant using cotton, used
Functional liquid sprayed 1 time per 3-4 days.
Step（1）Described liquor potassic permanganate, its mass concentration are 0.3%.
Step（1）Described functional liquid, wherein being respectively configured to sub-prime gauge part and being：
Water 100-120 parts, vitamin A 7-9 parts, vitamin C 5-8 parts, folic acid 1-3 parts, citric acid 1-3 parts, aloperine 0.5-
0.8 part, tazettine 0.1-0.2 parts.
Step（1）Described auxin solution, wherein gibberellin：Zeatin：Ethephon (CEPHA),2-(chloroethyl) phosphonic acid quality proportioning is 2-3:1:0.5-
0.8, phase mass concentration is 1%-2%.
Step（2）Described inhibitor, wherein tacrolimus：Aspirin：Endoxan quality proportioning is 2-3:2:
0.2-0.4, its mass concentration are 3%-5%.
The present invention has advantages below compared with prior art：Presprouting of seeds, seed is first respectively enterd into germicidal solution and life
Long plain solution, germ can be killed, improve seed cell activity, and immerse progress constant temperature vernalization in functional liquid, wherein what is contained is more
The compositions such as kind vitamin, plant acid and alkaloid, can improve seed cell metabolism, promote germination, and plant
Son can improve disease and insect resistance and resistance ability after absorbing.Method for culturing seedlings is downgraded, seed sowing carries out constant temperature nursery behind seedbed,
The Seed Germination and growth stage gradually steps up environmental stress, the increase of its growing environment resistance, stimulates the growth of seed resistance, mainly
Nutritional ingredient provides seed root and stem's cross growth, the longitudinal growth of seedling are restricted, and promotes iceberg Chinese rose to downgrade life
It is long, and the photosynthesis that high concentration carbon dioxide can improve seedling is horizontal, improves the absorption to inhibitor active ingredient, inhibitor
In containing compositions such as tacrolimus, aspirin and endoxan can play restriction effect to the growth of seedling, reduce seedling
Highly grow, realize the dwarfing of plant.Method for culturing seedlings is induced, the magnetic frequency by using alternately change magnetizes to seedling
Nursery, its caused magnetic field intensity constantly change, and in the case of the increase of growth of seedling resistance seedling can be stimulated to produce and adapt to environment
The tree characteristics of change, so as to reduce the growing height of plant.Post-processing method, seedling plants are subjected to semi-ring in stem
Cut, you can keep certain supply of the root nutrient to top, well-fed supply can be limited again, suppression is played to shoot growth
Effect.
Embodiment
Embodiment 1：
A kind of breeding method of iceberg Chinese rose dwarfted varieties, comprises the following steps：
（1）Presprouting of seeds：First iceberg Chinese rose seed is immersed in liquor potassic permanganate and soaks 2min, carries out detoxification treatment, is lowered
Pest and disease damage rate, then immerse in auxin solution and soak 1.5h, seed cell activity and differentiation capability are improved, then again soaks seed
Enter progress constant temperature vernalization in functional liquid, contain the compositions such as multivitamin, plant acid and alkaloid in functional liquid, seed can be improved
Cell metabolism is horizontal, promotes germination, and disease and insect resistance and resistance ability can be improved after neutron absorption, and temperature is
19 DEG C, time 20.5h, chitting piece is made；
（2）Downgrade nursery：Chitting piece is sowed into seedling bed and carries out nursery, 23 DEG C of temperature, humidity 61%, seed sprouting rank
Section, pressure 0.52Mpa, carbon dioxide volumetric concentration are 0.063%, spray 1 inhibitor, germination growth phase, pressure daily
Strong 0.82Mpa, carbon dioxide volumetric concentration are 0.105%, 1 inhibitor of sprinkling in every 2 days；Seedling can be improved by providing hyperbaric environment
External drag is grown, promotes seed root and stem's diameter increment, and high carbon dioxide concentration can improve the absorption to inhibitor,
The compositions such as its tacrolimus contained, aspirin and endoxan can play restriction effect to the growth of seedling；
（3）Induce nursery：First seedling is sprayed using auxin solution, then carries out magnetization induction, respectively frequency of use
For 0.34MHz and 0.52MHz, every 3 magnetization treatment 1 time, each 12min, the frequency checker frequency is 1 time/5min, is continuously entered
Row 11 days, obtains mutagenesis seedling；Its caused magnetic field intensity constantly changes, and seedling can be stimulated in the case of the increase of growth of seedling resistance
The tree characteristics for adapting to environmental change is produced, reduces the growing height of plant；
（4）Post-processing：When seedling is grown to 15cm, half ring cutting of every 0.6cm distances 1 time, can limit nutrition supplying of the root to top
Should, so as to limit plant height growth, every plant of knife of ring cutting 3, and parcel culture is carried out to plant using cotton, it is every using functional liquid
Spray 1 time within 3 days.
Step（1）Described liquor potassic permanganate, its mass concentration are 0.3%.
Step（1）Described functional liquid, wherein being respectively configured to sub-prime gauge part and being：
104 parts of water, 7.2 parts of vitamin A, 6 parts of vitamin C, 1.4 parts of folic acid, 1.5 parts of citric acid, 0.6 part of aloperine, narcissus
0.14 part of alkali.
Step（1）Described auxin solution, wherein gibberellin：Zeatin：Ethephon (CEPHA),2-(chloroethyl) phosphonic acid quality proportioning is 2:1:0.5, the phase
Mass concentration is 1.2%.
Step（2）Described inhibitor, wherein tacrolimus：Aspirin：Endoxan quality proportioning is 2:2:0.3,
Its mass concentration is 3.5%.
Embodiment 2：
（1）Presprouting of seeds：First iceberg Chinese rose seed is immersed in liquor potassic permanganate and soaks 3min, carries out detoxification treatment, is lowered
Pest and disease damage rate, then immerse in auxin solution and soak 2h, seed cell activity and differentiation capability are improved, then again immerses seed
Constant temperature vernalization is carried out in functional liquid, contains the compositions such as multivitamin, plant acid and alkaloid in functional liquid, it is careful that kind can be improved
Born of the same parents' metabolism, promote germination, and disease and insect resistance and resistance ability can be improved after neutron absorption, temperature 22
DEG C, time 21h, chitting piece is made；
（2）Downgrade nursery：Chitting piece is sowed into seedling bed and carries out nursery, 24 DEG C of temperature, humidity 64%, seed sprouting rank
Section, pressure 0.68Mpa, carbon dioxide volumetric concentration are 0.074%, spray 1 inhibitor, germination growth phase, pressure daily
Strong 0.95Mpa, carbon dioxide volumetric concentration are 0.11%, 1 inhibitor of sprinkling in every 2 days；Seedling life can be improved by providing hyperbaric environment
Long external drag, promote seed root and stem's diameter increment, and high carbon dioxide concentration can improve the absorption to inhibitor, its
The compositions such as the tacrolimus, aspirin and the endoxan that contain can play restriction effect to the growth of seedling；
（3）Induce nursery：First seedling is sprayed using auxin solution, then carries out magnetization induction, respectively frequency of use
For 0.38MHz and 0.57MHz, every 3 magnetization treatment 1 time, each 14min, the frequency checker frequency is 1 time/5min, is continuously entered
Row 12 days, obtains mutagenesis seedling；Its caused magnetic field intensity constantly changes, and seedling can be stimulated in the case of the increase of growth of seedling resistance
The tree characteristics for adapting to environmental change is produced, reduces the growing height of plant；
（4）Post-processing：When seedling is grown to 16cm, half ring cutting of every 0.8cm distances 1 time, can limit nutrition supplying of the root to top
Should, so as to limit plant height growth, every plant of knife of ring cutting 4, and parcel culture is carried out to plant using cotton, it is every using functional liquid
Spray 1 time within 4 days.
Step（1）Described liquor potassic permanganate, its mass concentration are 0.3%.
Step（1）Described functional liquid, wherein being respectively configured to sub-prime gauge part and being：
118 parts of water, 8.5 parts of vitamin A, 7 parts of vitamin C, 2.6 parts of folic acid, 2.5 parts of citric acid, 0.7 part of aloperine, narcissus
0.18 part of alkali.
Step（1）Described auxin solution, wherein gibberellin：Zeatin：Ethephon (CEPHA),2-(chloroethyl) phosphonic acid quality proportioning is 3:1:0.7, the phase
Mass concentration is 1.8%.
Step（2）Described inhibitor, wherein tacrolimus：Aspirin：Endoxan quality proportioning is 3:2:0.35,
Its mass concentration is 4.2%.
Contrast 1：
This contrast 1 does not carry out step compared with embodiment 1（1）、（5）Middle functional liquid uses, and other steps are same as Example 1.
Contrast 2：
This contrast 2 does not carry out step compared with embodiment 1（2）Nursery is downgraded, other steps are same as Example 1.
Contrast 3：
This contrast 3 does not carry out step compared with embodiment 2（3）Middle magnetization induction, other steps are same as Example 2.
Contrast 4：
This contrast 4 does not carry out step compared with embodiment 2（4）In half ring cutting handle, other steps are same as Example 2.
Contrast 5：
This contrast 5 individually to iceberg Chinese rose seedling spray inhibitor, other method without using.
Control group：
Control group is sprayed to iceberg Chinese rose using cycocel, and functional liquid is not used, downgrades nursery, magnetization induction, the processing of half ring cutting
And inhibitor.
To embodiment 1, embodiment 2, contrast 1, contrast 2, contrast 3, contrast 4, contrast 5 and control group experimental program, statistics
The iceberg Chinese rose cultivated its downgrade height, time at florescence, individual plant quantity of blooming are compared.
Experimental data：
| Project | Downgrade height cm | Time at florescence d | Individual plant is bloomed quantity piece |
| Embodiment 1 | 17.1 | 17 | 11 |
| Embodiment 2 | 17.4 | 16 | 10 |
| Contrast 1 | 16.5 | 15 | 9 |
| Contrast 2 | 14.5 | 16 | 10 |
| Contrast 3 | 15.2 | 15 | 9 |
| Contrast 4 | 16.3 | 16 | 10 |
| Contrast 5 | 15.7 | 15 | 9 |
| Control group | 25.3 | 11 | 6 |
Synthesis result：The dwarfing iceberg modern rose cultivarses that the inventive method is cultivated, compared with control group, downgrade height reduction
8.2cm, florescence time lengthening 6 days, individual plant bloom quantity improve 5.Using functional liquid and method for culturing seedlings is downgraded, height is downgraded and drops
Low 0.6cm, 2.6cm, florescence time lengthening 2 days, 1 day, individual plant bloom quantity improve 2,1；And use magnetization induction and suppression
Formulation method, downgrade height reduction 2.2cm, 1.7cm, florescence time lengthening 1 day, 2 days, individual plant bloom quantity improve 1,1.
Claims (5)
1. a kind of breeding method of iceberg Chinese rose dwarfted varieties, it is characterised in that comprise the following steps：
（1）Presprouting of seeds：First iceberg Chinese rose seed is immersed in liquor potassic permanganate and soaks 2-3min, then immerses auxin solution
Middle immersion 1-2h, then seed is immersed again constant temperature vernalization is carried out in functional liquid, temperature is 18-23 DEG C, time 20-22h, is made
Chitting piece；
（2）Downgrade nursery：Chitting piece is sowed into seedling bed and carries out nursery, 22-25 DEG C of temperature, humidity 60%-65%, seed
Sprouting stage, pressure 0.5-0.7Mpa, carbon dioxide volumetric concentration are 0.06%-0.08%, spray 1 inhibitor, seed daily
Germinating growth stage, pressure 0.8-1.0Mpa, carbon dioxide volumetric concentration are 0.1%-0.12%, spray 1 inhibitor within every 2 days；
（3）Induce nursery：First seedling is sprayed using auxin solution, then carries out magnetization induction, respectively frequency of use
For 0.3-0.4MHz and 0.5-0.6MHz, per 3h magnetization treatments 1 time, each 10-15min, the frequency checker frequency is 1 time/
5min, it is carried out continuously 10-12 days, obtains mutagenesis seedling；
（4）Post-processing：Half ring cutting of every 0.5-1cm distances 1 time, every plant of ring cutting 3-4 knife when seedling is grown to 14-17cm, and make
Parcel culture is carried out to plant with cotton, sprayed 1 time per 3-4 days using functional liquid.
2. the breeding method of iceberg Chinese rose dwarfted varieties as claimed in claim 1, it is characterised in that step（1）Described height
Potassium manganate solution, its mass concentration are 0.3%.
3. the breeding method of iceberg Chinese rose dwarfted varieties as claimed in claim 1, it is characterised in that step（1）Described work(
Can liquid, wherein being respectively configured to sub-prime gauge part and being：
Water 100-120 parts, vitamin A 7-9 parts, vitamin C 5-8 parts, folic acid 1-3 parts, citric acid 1-3 parts, aloperine 0.5-
0.8 part, tazettine 0.1-0.2 parts.
4. the breeding method of iceberg Chinese rose dwarfted varieties as claimed in claim 1, it is characterised in that step（1）Described life
Long plain solution, wherein gibberellin：Zeatin：Ethephon (CEPHA),2-(chloroethyl) phosphonic acid quality proportioning is 2-3:1:0.5-0.8, phase mass concentration are 1%-2%.
5. the breeding method of iceberg Chinese rose dwarfted varieties as claimed in claim 1, it is characterised in that step（2）Described suppression
Preparation, wherein tacrolimus：Aspirin：Endoxan quality proportioning is 2-3:2:0.2-0.4, its mass concentration are 3%-5%.
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