CN107409673A - The method for transplanting of non-irrigated tuber of pinellia tissue-cultured seedling - Google Patents
The method for transplanting of non-irrigated tuber of pinellia tissue-cultured seedling Download PDFInfo
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- CN107409673A CN107409673A CN201710313947.1A CN201710313947A CN107409673A CN 107409673 A CN107409673 A CN 107409673A CN 201710313947 A CN201710313947 A CN 201710313947A CN 107409673 A CN107409673 A CN 107409673A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
The invention discloses a kind of non-irrigated tuber of pinellia tissue culture method for transplanting, it is characterized in that, including steps such as seedbed cultivation, field-transplantings, wherein seedbed incubation step includes seedbed preparation, the preparation of EM insect prevention liquid and the transplanting of tissue culture bacterin case, the present invention can be transplanted according to the size fractionation of non-irrigated tuber of pinellia tissue-cultured seedling root stem tuber into each slurry case, and classification transplanting can effectively reduce field management and dig the integrated costs such as the manpower in time receiving.The inventive method is easy to the transport of tissue-cultured seedling during field-transplanting, transplants simple operation, saves the time, reduces labor intensity, while reduce seedling damage ratio and loss late.
Description
Technical field
The present invention relates to cultivation field, specifically a kind of field transplanting method of non-irrigated tuber of pinellia tissue-cultured seedling.
Background technology
The tuber of pinellia (Pinellia ternata), also known as ground text, keep field etc. category Arales, it is wild dark and damp in hillside, small stream side
Thick grass in or sylvan life, be distributed widely in the areas such as Yangtze River in China basin and North China.The non-irrigated tuber of pinellia is Araeceae perennial grass
The stem tuber of this plant, nature and flavor are pungent, warm, returns spleen, stomach, lung channel, and there is eliminating dampness and eliminating phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, dissolving lump and resolving mass, externally used detumescence to stop
Pain and other effects, be widely used simply, traditional parts of generic medicinal plants curative for effect.
In terms of domestic market, with the progress of medical science and technology and the scientific and technical innovation of traditional Chinese medicine, China there are about more than 2000 family's systems
New drug, special medicine and Chinese patent drug of the medicine group (factory) using the non-irrigated tuber of pinellia as main material production, about more than 500 plant, in addition all kinds of medicines
Material company, medicine materical crude slice company, medical institutions, clinic, pharmacy etc. about need 4500-5000 tons or so every year.Meanwhile Hong Kong, Macao and Taiwan market
Also largely from interior stock up every year, quantity is very big, and supply falls short of demand interiorly.After View of International Market, China joined WTO, pin
Channel is sold to widen, sales range expands, and the market share increases, Japan, South Korea, Southeast Asian countries to China's drought tuber of pinellia demand very
Yin, wherein an only Japanese country just occupies more than the 50% of the China's foreign trade total volume of exports, in 210 Chinese medicine preparations of Japan
Have about 50 of non-irrigated tuber of pinellia formula or so, meanwhile, Japan also with health foods such as non-irrigated tuber of pinellia processing medicine congee, medicine beverages, in recent years I
State's drought tuber of pinellia is wild with artificial yield and about 3000 tons or so, but the annual demand drought tuber of pinellia in domestic and international market is in 5500-
6000 tons or so, breach is big, is come out at the top in 500 kinds of Chinese medicines that China commonly uses.
Wild Rhizoma Pinelliae originates in China's most area, due to a variety of natural calamities such as excavation unordered for many years and arid waterlogging
Frequently, shown after national resources of natural crude drugs generaI investigation, wild non-irrigated tuber of pinellia yield is in the gesture of decline year by year；Non-irrigated tuber of pinellia artificial growth is
Have more than 30 years, the investigation of the national tuber of pinellia place of production finds slower development always, tuber of pinellia cultivation mainly using stem tuber breed early investment into
This height, sowing quantity is big, time-consuming, breeding coefficient is low, disease is heavier and production and extensive management Isoquant and quality are difficult to
Ensure, caused yield in trend of falling year by year；But the demand steady-state growth in two market both at home and abroad, imbalance between supply and demand day
It is beneficial sharp, develop non-irrigated tuber of pinellia semi-wild foster it is very urgent with artificial growth.
Tuber of pinellia introduces a collection for a long time using from it is numerous, reserve that mode is degenerated for tuber of pinellia stem tuber at present, the control of disease does not have also for one's own use
The solution of effect, modern biotechnology tissue culture propagation tuber of pinellia seedling is utilized for this, be to solve tuber of pinellia semi-wild to foster and manually
The kind source problem of plantation is effectively and one of important channel；Tissue culture method effectively reduces half by technical finesses such as virus-frees
The disease of summer sowing stem improves breeding coefficient, and be limited by equipment caused by tuber of pinellia seedling stem tissue culture production process, material, water power,
It is artificial to wait integrated cost higher, still there is not the actual production of scale, it is necessary to reduce cost in research and laboratory stage at present
Quality ability competitive superiority and actual application value are improved, it is contemplated that carry out the method for transplanting of a non-irrigated tuber of pinellia tissue-cultured seedling
Improve, reduce cost of labor to reduce labor intensity, improve the yield and quality of tuber of pinellia seedling stem, be the work of non-irrigated tuber of pinellia tissue culture seedling stem
Factory, standardize and offer technical support be provided.
The content of the invention
It is an object of the invention to provide a kind of method for transplanting of non-irrigated tuber of pinellia tissue-cultured seedling, this method can reduce labor intensity drop
Low production cost, and fertilising and the optimization of the techniques of pest control, improve the yield that non-irrigated tuber of pinellia seedling stem is cultivated with tissue-cultured seedling
And quality.
The present invention realizes that goal of the invention adopts the following technical scheme that：
Non-irrigated tuber of pinellia tissue culture method for transplanting, its feature is, comprises the following steps：
1) seedbed is cultivated
A) seedbed preparation
Mixed as nursery soil matrix ratios using sandy soil, perlite, peat soil (or garden mould), loaded after sterile-processed
Starch in case, keep soil matrix thickness 5.0cm~10.0cm；
B) preparation of EM insect prevention liquid
EM bacterium solutions, brown sugar, volume fraction 30%-35% alcohol, clear water are pressed 1：1：1：10 quality is placed in close than dispensing
Close container to ferment one week under the conditions of 25~35 DEG C, fermentation therebetween, when heaving because producing gas container, deflate in time, deflate
Immediately container is continued to seal afterwards, when fermenting and no longer produce to gas in container, and can smell sour-sweet fragrance, fermentation is completed, so
Closed put of the zymotic fluid after fermentation is saved backup at shady and cool dry afterwards；
C) tissue culture bacterin case is transplanted：
After the tuber of pinellia tissue-cultured seedling in tissue culture bottle grows to 5~8cm, uncap and add appropriate amounts of sterilized water, practice seedling room and place 2~3
My god, after taking out and rinsing the culture medium on tissue-cultured seedling well, its root is soaked in the 0.1mg/L methyl α-naphthyl acetate aqueous solution
10s, then it is transplanted in ready slurry case matrix soil, it is 40~60% to be positioned over sunshade rate, 15~25 DEG C of temperature, humidity
Tamed in the culture canopy of 40~50% condition, transplant seedlings and the EM bacterium of 1000 times of diluted concentration are a few days ago first sprayed into matrix soil
Insect prevention liquid (per 0.5~1L of case), after taming two weeks, then the insect prevention liquid to 1000 times of the diluted concentration of tissue-cultured seedling foliage-spray one time,
Removable loading tuber of pinellia seedling crop field after taming one month；
2) field-transplanting
After tissue-cultured seedling is growing one month in starching case, tissue-cultured seedling is transported to transplanting crop field together with slurry case and transplanted；
The field management of field planting process is implemented with reference to non-irrigated tuber of pinellia GAP technology, wherein in method of soil preparation, uses sufficient base manure,
After trench digging transplanting, when pouring root water while microbial-bacterial fertilizer (0.5kg-0.8kg/ mus), root water are imposed：Microbial-bacterial fertilizer weight
Than for 1:50；The EM bacterium solutions of 500~1000 times of diluted concentration are sprayed after transplanting one week, and 1 time (EM bacterium dosage is 0.25~0.75kg/
Mu), each growth cycle spray again 500~1000 times of diluted concentration EM bacterium insect prevention liquid 1~2 time (dosage is 0.1~
0.25kg/ mus).
Preferably, the seedbed, using open body structure, the bottom half is for mesh structure or provided with infiltration
Hole, the casing are filled in casing at oral area at least in a side wall provided with ventilative window, the soil matrix；
The bottom half is provided with step surface and is used to be superimposed up and down between casing and casing.
Preferably, the weight of the sandy soil, perlite, peat soil is 1:1:1.
Preferably, the slurry case is long 45~50cm, wide 35~40cm, high 16~20cm.
Preferably, tissue-cultured seedling forms the stem tuber sprout to differ in size in tissue culture bottle after culture of rootage, stem tuber can be pressed
(it is advanced to be more than 1cm to the size fractionation of diameter；0.5cm~1.0cm is middle rank；It is rudimentary less than 0.5cm), one is starched in case only
Transplant the tissue-cultured seedling of ad eundem；Advanced tissue-cultured seedling can be sold after domestication growth in starching case 1~2 month directly as seedling；In
The tissue-cultured seedling of level is most of all to grow to more than 1cm's after domestication growth in starching case is transplanted after January and grows half a year to seedling field
Stem tuber, it can rise to dig out and do seedling sale；Rudimentary tissue-cultured seedling is transplanted to seedling field growth 1 in domestication growth in starching case after January
Year above time can digging do seedling sale；The quantity of tissue-cultured seedling is 40~80 plants in each slurry case.
Preferably, the field-transplanting time is：Spring and autumn, 10~26 DEG C of outdoor temperature are advisable.
Preferably, the clear water is selected from limpid underground water.
Preferably, after the brown sugar is dissolved with hot water, water temperature is down to less than 45 DEG C and added in sealing container.
Compared with the prior art, the present invention has the beneficial effect that：
1st, the present invention is by tissue culture transplantation of seedlings in plastic box, and the transport of tissue-cultured seedling when being easy to field-transplanting, transplanting operation is just
Victory, the time is saved, reduce labor intensity, while reduce seedling damage ratio and loss late.
2nd, plastic crate of the present invention do slurry case it is reusable, saved cost, be easy to batch production, normalizing operation.
3rd, the present invention can be transplanted according to the size fractionation of non-irrigated tuber of pinellia tissue-cultured seedling root stem tuber into each slurry case.Tissue culture seedling rooting
The stem tuber sprout to differ in size is formd after culture, the size fractionation of stem tuber diameter can be pressed (more than 1 centimetre to be advanced；More than 0.5
Centimetre for middle rank；Less than 0.5 centimetre to be rudimentary), the slurry case by the tissue culture transplantation of seedlings of ad eundem to peer.Advanced tissue-cultured seedling exists
Starch after growth being tamed in case 1~2 month, seedling sale can be done, directly planted in crop field.The tissue-cultured seedling of middle rank tames life in case is starched
It is most of all to grow to more than 1 centimetre of stem tuber after transplanting grows half a year to seedling field after long January, it can rise to dig out and do seedling
Sell.Rudimentary tissue-cultured seedling is transplanted to seedling field in domestication growth in starching case after January needs the time of growth more than 1 year just to rise
Dig out and do seedling sale, classification transplanting effectively reduces field management and digs the integrated costs such as the manpower in time receiving.
4th, EM bacterium insect prevention liquid is sprayed in tissue-cultured seedling domestication incubation, survival rate more than 95%, grows shape in case is starched
Condition is good.
5th, in the cultivating process of seedling crop field, microbial-bacterial fertilizer is imposed, sprays EM bacterium solutions, EM bacterium insect prevention in growth course again
Liquid, soil micro-ecosystem balance is improved, improve soil fertility and the disease resistance of tissue-cultured seedling, seedling growing way is healthy and strong, bulbil setting percentage
Height, stem tuber are full, and seedling stem yield improves 5%~10% than existing field planting, reduces continuous cropping obstacle；Transplanting side of the invention
The improvement of method had not only promoted the growth of non-irrigated tuber of pinellia stem tuber but also had improved quality.
Brief description of the drawings
Fig. 1 is present invention slurry box structure schematic diagram.
Fig. 2 is present invention slurry case overlaying state schematic diagram up and down.
Fig. 3 is sectional view after slurry case is superimposed up and down.
Fig. 4 is another structure type slurry box structure schematic diagram of the present invention.
Fig. 5 is the sectional view after a kind of structure type slurry box structure of the present invention coordinates with cutting plate.
Embodiment
Explanation is further explained to technical solution of the present invention with reference to embodiments.
EM bacterium solutions in the embodiment of the present invention are commercial products, purchased from Linfen Yaodu District Fenhe River Amino Acid Factory, trade name
Claim：The prosperous biological bacterium solution of EM soil fertilities, microbial-bacterial fertilizer are purchased from Baoding Min get Fu new-type fertilizers Co., Ltd, trade name：Biology has
Machine fertilizer；Non-irrigated tuber of pinellia Standardized cultural technique is with reference to Chinese agricultural technology spread《Non-irrigated tuber of pinellia Standardized cultural technique》, author Du Zhanquan.
1) seedbed is cultivated
A) seedbed preparation
Mixed as nursery soil matrix ratios using sandy soil, perlite, peat soil (or garden mould), loaded after sterile-processed
Starch in case, keep soil matrix thickness 5.0cm~10.0cm；
B) preparation of EM insect prevention liquid
EM bacterium solutions, brown sugar, volume fraction 30%-35% alcohol, clear water (limpid underground water) are pressed 1：1：1：10 matter
Amount is placed in closed container and fermentation in one week is fermented under the conditions of 25~35 DEG C therebetween, when being heaved because producing gas container, than dispensing
Deflate in time, immediately container is continued to seal after deflation, ferment and no longer produced to gas in container, and sour-sweet fragrance can be smelt
When, fermentation is completed, and then saves backup closed put of the zymotic fluid after fermentation at shady and cool dry；After brown sugar is dissolved with hot water, water
Temperature drop is added in sealing container to less than 45 DEG C.
C) tissue culture bacterin case is transplanted：
After the tuber of pinellia tissue-cultured seedling in tissue culture bottle grows to 5~8cm, uncap and add appropriate amounts of sterilized water, practice seedling room and place 2~3
My god, after taking out and rinsing the culture medium on tissue-cultured seedling well, its root is soaked in the 0.1mg/L methyl α-naphthyl acetate aqueous solution
10s, then it is transplanted in ready slurry case matrix soil, it is 40~60% to be positioned over sunshade rate, 15~25 DEG C of temperature, humidity
Tamed in the culture canopy of 40~50% condition, transplant seedlings and the EM bacterium of 1000 times of diluted concentration are a few days ago first sprayed into matrix soil
Insect prevention liquid (per 0.5~1L of case), after taming two weeks, then the insect prevention liquid to 1000 times of the diluted concentration of tissue-cultured seedling foliage-spray one time,
Removable loading tuber of pinellia seedling crop field after taming one month；
2) field-transplanting
After tissue-cultured seedling is growing one month in starching case, tissue-cultured seedling is transported to transplanting crop field together with slurry case and transplanted；
The field management of field planting process is implemented with reference to non-irrigated tuber of pinellia GAP technology, wherein in method of soil preparation, uses sufficient base manure,
After trench digging transplanting, when pouring root water while microbial-bacterial fertilizer (0.5kg-0.8kg/ mus), root water are imposed：Microbial-bacterial fertilizer weight
Than for 1:50；The EM bacterium solutions of 500~1000 times of diluted concentration are sprayed after transplanting one week, and 1 time (EM bacterium dosage is 0.25~0.75kg/
Mu), each growth cycle spray again 500~1000 times of diluted concentration EM bacterium insect prevention liquid 1~2 time (dosage is 0.1~
0.25kg/ mus).
In the present embodiment, sandy soil, perlite, the weight of peat soil are 1:1:1.
In the present embodiment, tissue-cultured seedling forms the stem tuber sprout to differ in size in tissue culture bottle after culture of rootage, can be by block
(it is advanced to be more than 1cm to the size fractionation of stem diameter；0.5cm~1.0cm is middle rank；It is rudimentary less than 0.5cm), one is starched in case
Only transplant the tissue-cultured seedling of ad eundem；Advanced tissue-cultured seedling can be sold after domestication growth in starching case 1~2 month directly as seedling；
The tissue-cultured seedling of middle rank is most of all to grow to more than 1cm after domestication growth in starching case is transplanted after January and grows half a year to seedling field
Stem tuber, can rise to dig out and do seedling sale；Rudimentary tissue-cultured seedling is transplanted to seedling field in domestication growth in starching case after January to be grown
More than 1 year time can digging do seedling sale；The quantity of tissue-cultured seedling is 40~80 plants in each slurry case.
In the present embodiment, the field-transplanting time is：Spring and autumn, 10~26 DEG C of outdoor temperature are advisable.
As shown in figure 1, starch case for the embodiment of the present invention, it is long 45~50cm that casing 1, which starches case, wide 35~40cm, high by 16~
20cm, bottom are provided with water seepage hole or use high density mesh structure, and casing 1 is at least provided with oral area in a side wall
Netted ventilative window 2, soil matrix is filled in casing；The bottom of casing is provided with step surface and is used between casing and casing
Lower superposition, as Figure 2-3.
As shown in figure 4, it is the slurry case of another structure type of the embodiment of the present invention, the casing 1 is on the basis of above-mentioned casing
Opening slot 4 is provided with corresponding side wall, the opening slot 4 is engaged with cutting plate, for cutting plate to be inserted in slurry case, makes case
Hypothallus in body is cut into two parts up and down by cutting plate, in tissue-cultured seedling growth course, in order to preferably keep the water of matrix
Divide and nutrient, the thickness of matrix can reach 10cm, when tissue-cultured seedling is growing in starching case, its root only penetrates the upper half of hypothallus
The depth of part, substantially 3-5cm, when tissue-cultured seedling transplanting when, for the ease of by tissue-cultured seedling from slurry case in remove, can first by
In cutting plate insertion casing, matrix is divided into two parts up and down, is then directly shoveled to depth residing for cutting plate by scoop again, makes shovel
The shoveling depth of son can effectively be determined that the tissue-cultured seedling of carrying substrates can be removed by being then lifted up scoop again, both convenient,
Matrix will not be wasted because root institute carrying substrates are excessive again, and the matrix under cutting plate can be retained in casing with intact, can
Matrix for next batch of tissue-cultured seedling uses, and has saved matrix.
When it is implemented, cutting plate can use the alloy material of thinner thickness, and the oral area width of slot and cutting plate thickness phase
When starching case normal use, slot oral area can be sealed using rubber weather strip.
It should be understood that example as described herein and embodiment can be made only for explanation, those skilled in the art according to it
Various modifications or change, in the case where not departing from spirit of the invention, belong to protection scope of the present invention.
Claims (8)
1. non-irrigated tuber of pinellia tissue culture method for transplanting, it is characterised in that comprise the following steps：
1) seedbed is cultivated
A) seedbed preparation
Mixed using sandy soil, perlite, peat soil (or garden mould) as nursery soil matrix ratios, slurry case is loaded after sterile-processed
In, keep soil matrix thickness 5.0cm~10.0cm；
B) preparation of EM insect prevention liquid
EM bacterium solutions, brown sugar, volume fraction 30%-35% alcohol, clear water are pressed 1：1：1：10 quality is placed in closed appearance than dispensing
Device ferments fermentation in one week therebetween under the conditions of 25~35 DEG C, when being heaved because producing gas container, to deflate in time, after deflation immediately
Container is continued to seal, when fermenting and no longer produce to gas in container, and can smell sour-sweet fragrance, fermentation is completed, then will hair
Closed put of zymotic fluid after ferment saves backup at shady and cool dry；
C) tissue culture bacterin case is transplanted：
After the tuber of pinellia tissue-cultured seedling in tissue culture bottle grows to 5~8cm, uncap and add appropriate amounts of sterilized water, practice seedling room and place 2~3 days, take
After going out and rinsing the culture medium on tissue-cultured seedling well, 10s is soaked into its root in the 0.1mg/L methyl α-naphthyl acetate aqueous solution, so
After be transplanted in ready slurry case matrix soil, be positioned over sunshade rate as 40%~60%, 15 DEG C~25 DEG C of temperature, humidity
Tamed in the culture canopy of 40%~50% condition, transplant seedlings and the EM of 1000 times of diluted concentration is a few days ago first sprayed into matrix soil
Bacterium insect prevention liquid (per 0.5~1L of case), after taming two weeks, then the insect prevention to 1000 times of the diluted concentration of tissue-cultured seedling foliage-spray one time
Liquid, removable loading tuber of pinellia seedling crop field after taming one month；
2) field-transplanting
After tissue-cultured seedling is growing one month in starching case, tissue-cultured seedling is transported to transplanting crop field together with slurry case and transplanted；Crop field
The field management of planting process is implemented with reference to non-irrigated tuber of pinellia GAP technology, wherein in method of soil preparation, uses sufficient base manure, is transplanting
Afterwards, microbial-bacterial fertilizer (0.5kg-0.8kg/ mus), root water are imposed when pouring root water while：Microbial-bacterial fertilizer weight ratio is 1:
50；The EM bacterium solutions 1 time (EM bacterium dosage is 0.25~0.75kg/ mus) of 500~1000 times of diluted concentration are sprayed after transplanting one week, often
Individual growth cycle sprays the EM bacterium insect prevention liquid 1~2 time (dosage is 0.1~0.25kg/ mus) of 500~1000 times of diluted concentration again.
2. non-irrigated tuber of pinellia tissue-cultured seedling field transplanting method according to claim 1, it is characterised in that the seedbed is using open
Body structure, the bottom half is mesh structure or provided with water seepage hole, the casing at oral area at least at one
Side wall is provided with ventilative window, and the soil matrix is filled in casing；The bottom half portion is used for casing provided with step surface
It is superimposed up and down between casing.
3. non-irrigated tuber of pinellia tissue-cultured seedling field transplanting method according to claim 1, it is characterised in that the sandy soil, perlite,
The weight of peat soil is 1:1:1.
4. non-irrigated tuber of pinellia tissue-cultured seedling field transplanting method according to claim 1, it is characterised in that the slurry case for it is long by 45~
50cm, wide 35~40cm, high 16~20cm.
5. non-irrigated tuber of pinellia tissue-cultured seedling field transplanting method according to claim 1, it is characterised in that tissue-cultured seedling is in tissue culture bottle
The stem tuber sprout to differ in size is formd after culture of rootage, the size fractionation that can press stem tuber diameter (is advanced more than 1cm；0.5cm
~1.0cm is middle rank；It is rudimentary less than 0.5cm), one is starched the tissue-cultured seedling that ad eundem is only transplanted in case；Advanced tissue-cultured seedling exists
Starch after growth being tamed in case 1~2 month, can be sold directly as seedling；The tissue-cultured seedling of middle rank moves in domestication growth in starching case after January
Plant after growing half a year to seedling field, it is most of all to grow to more than 1cm stem tuber, it can rise to dig out and do seedling sale；Rudimentary
Tissue-cultured seedling in starching case domestication growth January after transplanting to seedling field grow more than 1 year time can digging do seedling sale；It is described
The quantity of tissue-cultured seedling is 40~80 plants in each slurry case.
6. non-irrigated tuber of pinellia tissue-cultured seedling field transplanting method according to claim 1, it is characterised in that the field-transplanting time is：
Spring and autumn, 10~26 DEG C of outdoor temperature are advisable.
7. non-irrigated tuber of pinellia tissue-cultured seedling field transplanting method according to claim 1, it is characterised in that the clear water is selected from limpid
Underground water.
8. non-irrigated tuber of pinellia tissue-cultured seedling field transplanting method according to claim 1, it is characterised in that the brown sugar hot water is molten
After change, water temperature is down to less than 45 DEG C and added in sealing container.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418465A (en) * | 2020-04-17 | 2020-07-17 | 毕节市农业科学研究所 | Pinellia ternata planting method |
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