CN107347815A - Utilize biological bacteria and the method for biological agent processing sulfurous method filter mud breeding earthworm - Google Patents
Utilize biological bacteria and the method for biological agent processing sulfurous method filter mud breeding earthworm Download PDFInfo
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- CN107347815A CN107347815A CN201710776048.5A CN201710776048A CN107347815A CN 107347815 A CN107347815 A CN 107347815A CN 201710776048 A CN201710776048 A CN 201710776048A CN 107347815 A CN107347815 A CN 107347815A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Abstract
Utilize biological bacteria and the method for biological agent processing sulfurous method filter mud breeding earthworm, 12 kilograms of compound biological bacterium is added by every cube of sulfurous method filter mud and is sufficiently mixed, heap chevron centipede space into strips again, every turning in 56 days 1 time, turning 25 times, fermentation temperature is controlled at 50 60 DEG C, is fermented 20 40 days, obtains the filter mud that ferments；After centipede space temperature drops to normal temperature, then take biological agent and fermentation filter mud in mass ratio 1:100 sprinklings, are fetched as earthworm bed substrate breeding earthworm after well mixed.Discarded object filter mud is handled using the biological agent of earthworm, while continuous propagation, the material in discarded object filter mud is constantly consumed and converted earthworm, you can realizes the minimizing of discarded object filter mud, innoxious and recycling.
Description
Technical field
The invention belongs to animal-breeding method field, and in particular to one kind utilizes biological bacteria and biological agent processing sulfurous acid
The method of method filter mud breeding earthworm.
Background technology
Earthworm is the common name to annelida oligochaeta class animal.In scientific classification, they belong to unidirectional earthworm mesh.Body
Body is cylindrical（Distinguished with the cylinder of round worm）, both sides are symmetrical, have metamerism：It is made up of more than 100 individual body segments,
After 11st section, the center back often saved has dorsal pore；There is no bone, belong to invertebrate, body surface is exposed, anoderm.
In addition to body first-half, remaining each section is respectively provided with bristle.Hermaphroditic, allogamy, ovum is produced by annulus during reproduction
Cocoon, breeding are of future generation.Being currently known earthworm has kind more than 2500, is referred to as by biologist Darwin on the earth within 1837 most valuable
The animal of value.
Earthworm is poikilotherm, and body temperature changes with the change of ambient temperature.Therefore, dependence of the earthworm to environment
Typically more more notable than homeothermal animal, environment temperature not only influences body temperature and the activity of earthworm, have an effect on earthworm metabolism,
Development and reproduction etc., and temperature also produces large effect to other living conditions, so as to influence earthworm indirectly.Cause
This, temperature is one of most important living condition of earthworm.
In general, the active temperature of earthworm is in the range of 5~30 DEG C, and 0~5 DEG C enters resting state, and less than 0 DEG C dead
Die, optimum temperature is 20~27 DEG C or so, now preferably can grow and breed.At 28~30 DEG C, one can be maintained
Fixed growth；Grow and stop at more than 32 DEG C；Activity is blunt at less than 10 DEG C；It is dead at more than 40 DEG C, earthworm cocoon hatching most suitable 18
~27 DEG C.It can be seen that the maximum fatal temperature of earthworm is less than other invertebrates.Plant preferably indoors, in southern area,
Summer ventilation moisturizing, as long as winter pass window insulation just can whole year normally production（Here temperature refers to the temperature of base-material, because air
Temperature and the temperature of base-material be different, such as：When 0 DEG C of air themperature, base temperature has 12 DEG C or so；Work as Air Temperature
Spend for 38 DEG C when, base temperature but only have 28 DEG C or so because excrement material contains high moisture）.
The motion of earthworm and excreta are highly profitable to the quality for improving soil, and the gas permeability of soil can be made to keep good,
Make soil conservation health status, agricultural is played an important role.One healthy earthworm can overturn 20~40 in one acre every year
Ton soil.
Earthworm energy chesson, the increase soil organism simultaneously improve structure, moreover it is possible to promote during acid or basic soil is changed into
Property soil, the quick-acting compositions of increase phosphorus etc., soil is suitable to the growth of crops.Because earthworm contains abundant protein, therefore,
Feed as poultry, fowl and culture fishery, the effect of volume increase can be obtained.Earthworm is pharmaceutically also occupying certain ground
Position, is common Chinese medicine, have antipyretic, antispastic, it is active, relieving asthma, be depressured and the effect such as diuresis.
The specific enzymes of a kind of energy decomposing protein, fat and wood fibre, therefore, leaf, rice can be secreted out of in earthworm body
Grass, feces of livestock and poultry, house refuse, activated sludge and papermaking, the leftover bits and pieces etc. of food industry, can be its foodstuffs.Earthworm
Environmental pollution can be eliminated to a certain extent.Therefore, many countries have all set up vermiculture factory, and vermiculture work
Factory is referred to as " environmental purification device ".The trace meters such as mercury, lead and the cadmium in soil, this metalloid element can be absorbed due to earthworm
Aggregate amount in earthworm body is 10 times of extraneous content.Therefore, some scientists think that earthworm can be used as heavy metal in soil
The monitoring animal of pollution.
Bagasse is the Main By product of cane sugar manufacture industry, wherein containing cellulose 32%~48%, hemicellulose 19%
~24%, lignin 23%~32%, protein about 2%, ash content about 2%~3%.2012 ~ 2013 squeeze season, the processing of Guangxi sugarcane
Measure 75,000,000 tons, bagasse to sugarcane than about 23% (moisture content is about 48%), about produce 17,000,000 tons of bagasse, filter mud yield
(dry filter mud meter) is about 970,000 tons.The comprehensive utilization of bagasse is cane sugar manufacture enterprise development recycling economy, increase economic efficiency,
Realize the important step of sugar industry sustainable development；Sulfurous method filter mud is that sugar-cane juice is clarified in the peace and quiet process of sugar refining technology
The residue discharged afterwards by filter press or vacuum suction filter.In sub- method filter mud in addition to containing substantial amounts of sugarcane fibre, also rich in easy
Nutriment needed for the biological growths such as degradation of organic substances, nitrogen, phosphorus, calcium, potassium.At present, sulfurous method production cane sugar factory filter mud
In addition to sub-fraction is used to produce compoiste fertilizer, a part is used to produce the animal feeds such as pig, ox, chicken, duck, fish, also has
A part directly discharges farmland, other substantially all in discarded state, causes serious problem of environmental pollution.
Earthworm biological treatment of sludge technology is a kind of method that sludge is handled using the microorganism artificial ecological system of earthworm one
, it utilizes the ecological functions of earthworm, is finally reached mud decrement, stable and recycling, and dealing with earthworm sludge technology has place
The features such as effect is good, process equipment is simple, maintenance management is convenient, obvious environment benefit, energy consumption are low, expense is lacked is managed, numerous
Sludge disposal technology in have its unique advantage.
The content of the invention
The method that the present invention produces the discarded object filter mud breeding earthworm of white sugar using carbonatation process, using the biological agent of earthworm
Discarded object filter mud is handled, while continuous propagation, the material in discarded object filter mud is constantly consumed and converted earthworm,
The minimizing of discarded object filter mud, innoxious and recycling can be achieved.
The technical solution adopted in the present invention is：
A kind of method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent, it comprises the following steps：
（1）1-2 kilograms of compound biological bacterium is added by every cube of sulfurous method filter mud and is sufficiently mixed, then heap chevron pile into strips
Body, per turning in 5-6 days 1 time, turning 2-5 times, fermentation temperature is controlled at 50-60 DEG C, is fermented 20-40 days, obtains the filter mud that ferments；Treat
After centipede space temperature drops to normal temperature, then take biological agent and fermentation filter mud in mass ratio 1:100 sprinklings, earthworm bed is obtained after well mixed
Matrix；
（2）It is vermiculture feed lot that low yield, which abandons waste field, in transformation, and weeding is carried out to place, rolls leveling, surrounding digs good row
Ditch, line-spacing is 40~45cm between earthworm bed, line-spacing center excavate it is wide, deep be 25~30cm soil box, earthworm bedside 130~
150cm, high 35~40cm, upper strata are covered using agricultural crop straw, and a height of 20~40cm steps are laid by lower floor（1）Obtained earthworm bed
Matrix；
（3）Launch commodity earthworm seedling or kind earthworm seedling, injected volume are controlled in every mu of 300~400kg；
（4）Daily management：Summer earthworm bed substrate thickness is maintained at 35~40cm, carry out sunshade and diligent watering work with adjust temperature,
Humidity, 1~2 day in dusk watering 1 time；Winter earthworm bed substrate thickness is maintained at 30~40cm, waters once within 5~10 days, 20~
Add new earthworm bed substrate 1 time within 30 days；Controlling environment temperature on ordinary days, earthworm bed substrate water content is 60 ~ 75% at 18 ~ 26 DEG C；
（5）Feed is fed, monthly 2 times, earthworm bed is first turned over before feeding, using heap block upslide method, thickness is 10 centimetres, should not be by bed surface
Gai Man, do not ask smooth, to separate earthworm；
（6）Harvesting, harvest 4~6 times within 1 year, 40~50 days summers harvested once, and spring, 60~80 days autumns harvest once, every time
Earthworm bed substrate is supplemented after harvesting, and adjusts cultivation density, kind earthworm will update once every year, and culturing bed changes once every year.
Sulfurous method filter mud refers to that cane sugar factory is carried out during sugaring using lime and sulfur dioxide as fining agent
Filter mud caused by cane juice clarification processing.In sugar manufacturing process, due to the protein contained by mixed juice, pectic substance, organic
The non-sugar organic substance such as acid is unfavorable to cane sugar boiling and crystal, therefore, must carry out clarifying treatment to mixed juice.Hydrogen is added to mixed juice
The materials such as calcium oxide, sulfur dioxide, phosphoric acid, the non-sugar organic matter such as protein, pectic substance, organic acid is precipitated, precipitate
Thing filter residue, referred to as filter mud as obtained from filter press or vacuum suction filter or centrifugal separation equipment, it is the big of cane sugar manufacture
One of ancestor's byproduct.
Filter mud contains sugarcane fibre, sucrose, the condensation colloid with cerosin, protein, calcium phosphate, also carries secretly with sugarcane band
The sand come, wherein most compositions come from sugarcane, calcareous thing majority adds when cane juice clarification is handled.With regard to physical property
Speech, filter mud are soft, the materials slightly like sponge, have porous, color is to be yellowish, dun down to black.Can be with when drying
Substantial amounts of moisture is absorbed immediately.The filter mud moisture content about 50-70% just to dispatch from the factory, after long-term natural drying in an atmosphere, moisture
Content may decrease to 15%.The weight very little of the unit volume of filter mud, the filter mud per ton for containing 65% moisture, its volume account for 1.6m
³.The composition of different sugar refining technology filter muds is as shown in the table：
The carbon-nitrogen ratio of sulfurous method filter mud（Refer to the ratio of the total content of the total content of carbon and nitrogen in organic matter）It is more beneficial for earthworm
The cultivation of earthworm.
The height of centipede space is 70-100 centimetres.
Compound biological bacterium is made up of dissolving phosphor and dissolving potassium bacterium, geotrichum candidum, photosynthetic bacteria and saccharomycete, and their weight proportion is
2.8-3:0.6-1.4:2.2-2.8:1.2-1.8, it is put into by corn flour 10-18%, peptone 2-6%, calcium carbonate 2-6%, sucrose 6-
14% and surplus for pure water culture medium in cultivate, fermented 8-12 days at 23-28 DEG C.
Dissolving phosphor and dissolving potassium bacterium can independently carry out fixed nitrogen in sulfurous method filter mud, have stronger nitrogen fixing capacity, and energy
Auxin is enough secreted, promotes the growth and development of earthworm.Dissolving phosphor and dissolving potassium bacterium can by sulfurous method filter mud not by earthworm absorb
Invalid potassium and invalid phosphorus, being converted into can be by available potassium and rapid available phosphorus that earthworm absorbs, while releases in effect sulfurous method filter mud
The multiple elements such as silicon, manganese, zinc, molybdenum, improve trophic level；Potassium decomposing, phosphate-solubilizing bacteria produce gibberellin, cell point in vital movement
The bioactive substances such as element, heteroauxin are split, effectively earthworm can be stimulated to grow.
Geotrichum candidum, English scientific name：Geotrichum candidum, it is Deuteromycotina, Hyphomycetes, hyphomycetales, Moniliaceae,
Geotrichum, fungi are a kind of.For the morphological feature of geotrichum candidum between saccharomycete and mould, modes of reproduction is a small number of based on fragmentation
There is blastopore between bacterial strain.For growth temperature range at 5 DEG C -38 DEG C, optimum growth temperature is 25 DEG C.Growth pH scopes exist
3-11, optimal pH 5-7, there is extensive annidation.Individual plant geotrichum candidum has a certain degree of phenotype changeability, of the same race
Genetic polymorphism is presented in interior different strains, and colony colour is from white to cream color, and a small number of bacterial strains are light brown or dark brown, quality
From grease to epithelium shape.Geotrichum candidum energy protein hydrolysate, wherein majority can liquefy gelatin, peptonized milk, minority can only peptonized milk,
Can not be liquefied gelatin.For this bacteria growing temperature range at 5 DEG C ~ 38 DEG C, optimum temperature is 25 DEG C.PH scopes are grown in 3-11, it is most suitable
PH is 5-7.The mycoprotein nutritive value of geotrichum candidum is high, can edible and feed use, it can also be used to extract nucleic acid.Geotrichum candidum is also
Energy synthctic fat, the organic wastewater production forage protein in sugar refinery, brewery and other food factories can be utilized.
Photosynthetic bacteria (English name：Photosynthetic Bacteria Abbr.name:PSB), it is to go out earliest on the earth
Now there are the prokaryotes of original luminous energy synthetic system, be do not put the photosynthetic bacterium of oxygen under anaerobic total
Claim, it is widely present in global biosphere everywhere.In aquaculture, nitrite, sulfide in the water body that can degrade
Deng noxious material, realization serve as bait, purify water, prevention disease, as the functions such as feed addictive, its many characteristics.
If photosynthetic bacteria breeds, other beneficial microbes can also breed.The ammonia that the dissolving phosphor and dissolving potassium bacterium of the application is secreted with photosynthetic bacteria
Base acid is bait, and it can dissolve insoluble phosphorus, and can and nitrogen-fixing bacteria symbiosis, significantly improve its nitrogen fixing capacity.This is photosynthetic thin
Bacterium is by being separated in nature and cultivating pool, and utilizes modern biotechnology, is expanded by artificially breeding and breeding
Liquid made of increasing and solid biologic preparation.
Saccharomycete is some unicellular fungis, can be survived in anaerobic environment, maximum a kind of micro- of the direct amount of the mankind
Biology.Form generally has spherical, oval, sausage shape, ellipse, lemon shape or rhizoma nelumbinis shape etc., generally 1 ~ 5 micron ' 5 ~ 30
Micron.There are cell membrane, cell membrane, nucleus, cytoplasmic domains.Saccharomycete is mainly grown in the moist of slant acidity and contains sugared environment
In, it is known to primary yeast more than 1000, sporogenic ability is produced according to saccharomycete, yeast can be divided into three classes：Form the strain of spore
System belongs to sac fungus and basidiomycetes.Yeast thalline contains abundant protein, fat, sugar and B family vitamin etc., and enzyme,
Coenzyme, ribonucleic acid, sterol and some metabolic intermediate products, the nutritive value of fermented food can be improved.
Biological agent is Fructus Chaenomelis ferment or polyphenol oxidase, and polyphenol oxidase is taken containing cellulase, pectase, neutral egg
One or more in white enzyme.
Fructus Chaenomelis ferment（Papain）It is to maintain the normal work(of body to be present in a kind of proteolytic enzyme in pawpaw
A kind of necessary material of the vital movements such as food, repair tissue, can be digested.It is also autonomic drug while pawpaw is food, they
Reason with integration of drinking and medicinal herbs.Fructus Chaenomelis ferment can be helped digest, absorbed, can this is three big with decomposing protein, carbohydrate and fat
The plant of nutrient, especially clear stool expelling intestinal toxin and decomposition waist and belly fat significant effect, this is the main work(of Fructus Chaenomelis ferment
Energy.The raw pawpaw of Fructus Chaenomelis ferment preparation method is smashed, add 5-8 times of water, add raw pawpaw percentage by weight 2%-3% salt and
3-5% rice vinegar, insert terrine and pickle 10-15 days, be filtrated to get Fructus Chaenomelis ferment.
Polyphenol oxidase（Outer literary fame：polyphenol oxidase）It is a kind of proteosome, in tea tree vital movement and tea
Participate in a series of chemical changes as caused by enzymatic activity in leaf process, therefore the biocatalyst that is otherwise known as.Polyphenol oxygen
Change enzyme（,PPO）It is a kind of metalloproteinases that distributed pole is wide in nature, is prevalent in the plastid of plant, fungi, insect
In, or even may detect that on the plant residue of soil rot the activity of polyphenol oxidase.Because its is easy to detect, it is
One of several fermentoids studied earliest.Being hardened from Yoghid discovery japanese lacquer tree juices in 1883 may be with certain active material phase
Close, Keilin D. and Mann G. have studied the extraction and purifying of mushroom polyphenol oxidase within 1938, obtain polyphenol oxidase simultaneously
This fermentoid is referred to as polyphenol oxidase.Polyphenol oxidase is also known as catechol-oxydase, tyrosinase, phenol enzyme, first
Phenolase, catechol oxidoreducing enzyme, it is the first major class oxidoreducing enzyme in six big fermentoids.
The application is by biological agent（Fructus Chaenomelis ferment or polyphenol oxidase）It is sprayed onto in fermentation filter mud, promotes mutually, jointly
The ferment ecosystem with pluralistic function of a complexity and stabilization is formed, harmful microorganism, especially pathogen can be suppressed
With the activity of putrefactivebacteria, so as to promote the effect of earthworm fast-growth.
Step（5）In, the feed fed is that mass ratio is 8:5:2 fowl and animal excrement, agricultural crop straw, corn starch sugar dreg
The compound obtained after everfermentation.
Agricultural crop straw is one or more of mixing in maize straw, wheat stalk, rice straw or soybean stalk.
Fowl and animal excrement is one or more mixing in cow dung, horsehit, pig manure and chicken manure.
Corn starch sugar dreg is that cornstarch filters in sugar industry or sugaring workshop section of fermentation enterprise after double-enzyme method sugar making
The filter residue that liquid glucose obtains.
Beneficial effects of the present invention：
1st, multiple beneficial bacterium is mixed and made into compound biological bacterium by the application, and having improves breeding environment and promote earthworm to grow
Effect, each bacterial strain become apparent from by cooperative compensating functionally, its effect compared to single bacterial strain；
2nd, the present invention is using biological bacteria and the method for biological agent processing sulfurous method filter mud breeding earthworm, using the biology of earthworm
Effect is handled discarded object filter mud, earthworm while continuous propagation, the material in discarded object filter mud be constantly consumed and
Conversion, you can realize the minimizing of discarded object filter mud, innoxious and recycling；
3rd, using the earthworm bed substrate of uniqueness, not only discarded object is re-used, protects environment, and contained nutritional ingredient
Enriching, there is preferable permeability, undesirable microorganism, pathogen, the miscellaneous bacteria source of infection and hazard residue material equal size are less,
Earthworm growing environment is improved, reduces morbidity and mortality, improves the speed of growth and yield；
4th, the dissolving phosphor and dissolving potassium bacterium of the application is using the amino acid that photosynthetic bacteria is secreted as bait, and it can dissolve insoluble phosphorus, and can with
Nitrogen-fixing bacteria symbiosis, its nitrogen fixing capacity is significantly improved, while releases the multiple element such as silicon, manganese, zinc, molybdenum in effect sulfurous method filter mud,
Trophic level is improved, the demand of inorganic elements and organic matter in being grown supplemented with earthworm；
5th, potassium decomposing, phosphate-solubilizing bacteria produce the bioactive substances such as gibberellin, the basic element of cell division, heteroauxin in vital movement,
Effectively earthworm can be stimulated to grow；
6th, the application is by biological agent（Fructus Chaenomelis ferment or polyphenol oxidase）It is sprayed onto in fermentation filter mud, promotes mutually, common structure
Into a complexity and the stable ferment ecosystem with pluralistic function, can suppress harmful microorganism, especially pathogen with
The activity of putrefactivebacteria, so as to promote the effect of earthworm fast-growth；
7th, it is vermiculture feed lot that low yield, which abandons waste field, in transforming in addition, solves some areas and is difficult to asking for breeding earthworm
Topic so that vermiculture regulation and standardization, promote the development of vermiculture industry；
8th, present invention process step is simple, abundant raw material, cheap and easy to get, and management is convenient, promotes the development of vermiculture industry, and
Big, bright in colour, the internal nonhazardous element residual of earthworm individual of output, nutritive value are high, have preferable economic value.
Embodiment
Embodiment 1：
A kind of method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent, it comprises the following steps：
（1）1 kilogram of compound biological bacterium is added by every cube of sulfurous method filter mud and is sufficiently mixed, then heap chevron centipede space into strips,
The height of centipede space is 70 centimeters, and per turning in 5-6 days 1 time, turning 2-5 times, fermentation temperature is controlled at 50-60 DEG C, and ferment 20-40
My god, obtain the filter mud that ferments；After centipede space temperature drops to normal temperature, then take biological agent and fermentation filter mud in mass ratio 1:100 sprinklings,
Earthworm bed substrate is obtained after well mixed；
（2）It is vermiculture feed lot that low yield, which abandons waste field, in transformation, and weeding is carried out to place, rolls leveling, surrounding digs good row
Ditch, line-spacing is 40~45cm between earthworm bed, line-spacing center excavate it is wide, deep be 25~30cm soil box, earthworm bedside 130~
150cm, high 35~40cm, upper strata are covered using agricultural crop straw, and lower floor spreads a height of 20~40cm steps（1）Obtained earthworm bed base
Matter；
（3）Launch commodity earthworm seedling or kind earthworm seedling, injected volume are controlled in every mu of 300~400kg；
（4）Daily management：Summer earthworm bed substrate thickness is maintained at 35~40cm, carry out sunshade and diligent watering work with adjust temperature,
Humidity, 1~2 day in dusk watering 1 time；Winter earthworm bed substrate thickness is maintained at 30~40cm, waters once within 5~10 days, 20~
Add new earthworm bed substrate 1 time within 30 days；Controlling environment temperature on ordinary days, earthworm bed substrate water content is 60 ~ 75% at 18 ~ 26 DEG C；
（5）Feed is fed, monthly 2 times, earthworm bed is first turned over before feeding, using heap block upslide method, thickness is 10 centimetres, should not be by bed surface
Gai Man, do not ask smooth, to separate earthworm；
（6）Harvesting, harvest 4~6 times within 1 year, 40~50 days summers harvested once, and spring, 60~80 days autumns harvest once, every time
Earthworm bed substrate is supplemented after harvesting, and adjusts cultivation density, kind earthworm will update once every year, and culturing bed changes once every year.
Sulfurous method filter mud refers to that cane sugar factory is carried out during sugaring using lime and sulfur dioxide as fining agent
Filter mud caused by cane juice clarification processing.
Compound biological bacterium is made up of dissolving phosphor and dissolving potassium bacterium, geotrichum candidum, photosynthetic bacteria and saccharomycete, and their weight proportion is
2.8-3:0.6-1.4:2.2-2.8:1.2-1.8, it is put into by corn flour 10-18%, peptone 2-6%, calcium carbonate 2-6%, sucrose 6-
14% and surplus for pure water culture medium in cultivate, fermented 8-12 days at 23-28 DEG C.
Biological agent is Fructus Chaenomelis ferment.The raw pawpaw of Fructus Chaenomelis ferment preparation method is smashed, and adds 5-8 times of water, adds raw wood
Melon percentage by weight 2%-3% salt and 3-5% rice vinegar, insert terrine and pickle 10-15 days, be filtrated to get Fructus Chaenomelis ferment.
Step（5）In, the feed fed is that mass ratio is 8:5:2 fowl and animal excrement, agricultural crop straw, corn starch sugar dreg
The compound obtained after everfermentation.
Agricultural crop straw is maize straw and wheat stalk by weight 1:1 mixing.
The fowl and animal excrement is cow dung and horsehit by weight 1:1 mixing mixing.
Corn starch sugar dreg is that cornstarch filters in sugar industry or sugaring workshop section of fermentation enterprise after double-enzyme method sugar making
The filter residue that liquid glucose obtains.
Embodiment 2：
A kind of method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent, it comprises the following steps：
（1）1.5 kilograms of compound biological bacterium is added by every cube of sulfurous method filter mud and is sufficiently mixed, then heap chevron pile into strips
Body, the height of centipede space is 80 centimeters, and per turning in 5-6 days 1 time, turning 2-5 times, fermentation temperature is controlled at 50-60 DEG C, and ferment 20-
40 days, obtain the filter mud that ferments；After centipede space temperature drops to normal temperature, then take biological agent and fermentation filter mud in mass ratio 1:100 sprays
Spill, earthworm bed substrate is obtained after well mixed；
（2）It is vermiculture feed lot that low yield, which abandons waste field, in transformation, and weeding is carried out to place, rolls leveling, surrounding digs good row
Ditch, line-spacing is 40~45cm between earthworm bed, line-spacing center excavate it is wide, deep be 25~30cm soil box, earthworm bedside 130~
150cm, high 35~40cm, upper strata are covered using agricultural crop straw, and lower floor spreads a height of 20~40cm steps（1）Obtained earthworm bed base
Matter；
（3）Launch commodity earthworm seedling or kind earthworm seedling, injected volume are controlled in every mu of 300~400kg；
（4）Daily management：Summer earthworm bed substrate thickness is maintained at 35~40cm, carry out sunshade and diligent watering work with adjust temperature,
Humidity, 1~2 day in dusk watering 1 time；Winter earthworm bed substrate thickness is maintained at 30~40cm, waters once within 5~10 days, 20~
Add new earthworm bed substrate 1 time within 30 days；Controlling environment temperature on ordinary days, earthworm bed substrate water content is 60 ~ 75% at 18 ~ 26 DEG C；
（5）Feed is fed, monthly 2 times, earthworm bed is first turned over before feeding, using heap block upslide method, thickness is 10 centimetres, should not be by bed surface
Gai Man, do not ask smooth, to separate earthworm；
（6）Harvesting, harvest 4~6 times within 1 year, 40~50 days summers harvested once, and spring, 60~80 days autumns harvest once, every time
Earthworm bed substrate is supplemented after harvesting, and adjusts cultivation density, kind earthworm will update once every year, and culturing bed changes once every year.
Sulfurous method filter mud refers to that cane sugar factory is carried out during sugaring using lime and sulfur dioxide as fining agent
Filter mud caused by cane juice clarification processing.
Compound biological bacterium is made up of dissolving phosphor and dissolving potassium bacterium, geotrichum candidum, photosynthetic bacteria and saccharomycete, and their weight proportion is
2.8-3:0.6-1.4:2.2-2.8:1.2-1.8, it is put into by corn flour 10-18%, peptone 2-6%, calcium carbonate 2-6%, sucrose 6-
14% and surplus for pure water culture medium in cultivate, fermented 8-12 days at 23-28 DEG C.
Biological agent is polyphenol oxidase, contains pectase and neutral proteinase.
Step（5）In, the feed fed is that mass ratio is 8:5:2 fowl and animal excrement, agricultural crop straw, corn starch sugar dreg
The compound obtained after everfermentation.
Agricultural crop straw is rice straw and soybean stalk by weight 1:1 mixing mixing.
The fowl and animal excrement is pig manure and chicken by weight 1:1 mixing mixing.
Corn starch sugar dreg is that cornstarch filters in sugar industry or sugaring workshop section of fermentation enterprise after double-enzyme method sugar making
The filter residue that liquid glucose obtains.
Embodiment 3：
A kind of method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent, it comprises the following steps：
（1）2 kilograms of compound biological bacterium is added by every cube of sulfurous method filter mud and is sufficiently mixed, then heap chevron centipede space into strips,
The height of centipede space is 90 centimeters, and per turning in 5-6 days 1 time, turning 2-5 times, fermentation temperature is controlled at 50-60 DEG C, and ferment 20-40
My god, obtain the filter mud that ferments；After centipede space temperature drops to normal temperature, then take biological agent and fermentation filter mud in mass ratio 1:100 sprinklings,
Earthworm bed substrate is obtained after well mixed；
（2）It is vermiculture feed lot that low yield, which abandons waste field, in transformation, and weeding is carried out to place, rolls leveling, surrounding digs good row
Ditch, line-spacing is 40~45cm between earthworm bed, line-spacing center excavate it is wide, deep be 25~30cm soil box, earthworm bedside 130~
150cm, high 35~40cm, upper strata are covered using agricultural crop straw, and lower floor spreads a height of 20~40cm steps（1）Obtained earthworm bed base
Matter；
（3）Launch commodity earthworm seedling or kind earthworm seedling, injected volume are controlled in every mu of 300~400kg；
（4）Daily management：Summer earthworm bed substrate thickness is maintained at 35~40cm, carry out sunshade and diligent watering work with adjust temperature,
Humidity, 1~2 day in dusk watering 1 time；Winter earthworm bed substrate thickness is maintained at 30~40cm, waters once within 5~10 days, 20~
Add new earthworm bed substrate 1 time within 30 days；Controlling environment temperature on ordinary days, earthworm bed substrate water content is 60 ~ 75% at 18 ~ 26 DEG C；
（5）Feed is fed, monthly 2 times, earthworm bed is first turned over before feeding, using heap block upslide method, thickness is 10 centimetres, should not be by bed surface
Gai Man, do not ask smooth, to separate earthworm；
（6）Harvesting, harvest 4~6 times within 1 year, 40~50 days summers harvested once, and spring, 60~80 days autumns harvest once, every time
Earthworm bed substrate is supplemented after harvesting, and adjusts cultivation density, kind earthworm will update once every year, and culturing bed changes once every year.
Sulfurous method filter mud refers to that cane sugar factory is carried out during sugaring using lime and sulfur dioxide as fining agent
Filter mud caused by cane juice clarification processing.
Compound biological bacterium is made up of dissolving phosphor and dissolving potassium bacterium, geotrichum candidum, photosynthetic bacteria and saccharomycete, and their weight proportion is
2.8-3:0.6-1.4:2.2-2.8:1.2-1.8, it is put into by corn flour 10-18%, peptone 2-6%, calcium carbonate 2-6%, sucrose 6-
14% and surplus for pure water culture medium in cultivate, fermented 8-12 days at 23-28 DEG C.
Biology enzyme is polyphenol oxidase, contains cellulase and pectase.
Step（5）In, the feed fed is that mass ratio is 8:5:2 fowl and animal excrement, agricultural crop straw, corn starch sugar dreg
The compound obtained after everfermentation.
Agricultural crop straw is wheat stalk and rice straw by weight 1:1 mixing mixing.
The fowl and animal excrement is horsehit and pig manure by weight 1:1 mixing mixing.
Corn starch sugar dreg is that cornstarch filters in sugar industry or sugaring workshop section of fermentation enterprise after double-enzyme method sugar making
The filter residue that liquid glucose obtains.
Embodiment 4：
A kind of method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent, it is characterised in that it includes
Following steps：
（1）1.5 kilograms of compound biological bacterium is added by every cube of sulfurous method filter mud and is sufficiently mixed, then heap chevron pile into strips
Body, the height of centipede space is 100 centimeters, and per turning in 5-6 days 1 time, turning 2-5 times, fermentation temperature is controlled at 50-60 DEG C, and ferment 20-
40 days, obtain the filter mud that ferments；After centipede space temperature drops to normal temperature, then take biological agent and fermentation filter mud in mass ratio 1:100 sprays
Spill, earthworm bed substrate is obtained after well mixed；
（2）It is vermiculture feed lot that low yield, which abandons waste field, in transformation, and weeding is carried out to place, rolls leveling, surrounding digs good row
Ditch, line-spacing is 40~45cm between earthworm bed, line-spacing center excavate it is wide, deep be 25~30cm soil box, earthworm bedside 130~
150cm, high 35~40cm, upper strata are covered using agricultural crop straw, and lower floor spreads a height of 20~40cm steps（1）Obtained earthworm bed base
Matter；
（3）Launch commodity earthworm seedling or kind earthworm seedling, injected volume are controlled in every mu of 300~400kg；
（4）Daily management：Summer earthworm bed substrate thickness is maintained at 35~40cm, carry out sunshade and diligent watering work with adjust temperature,
Humidity, 1~2 day in dusk watering 1 time；Winter earthworm bed substrate thickness is maintained at 30~40cm, waters once within 5~10 days, 20~
Add new earthworm bed substrate 1 time within 30 days；Controlling environment temperature on ordinary days, earthworm bed substrate water content is 60 ~ 75% at 18 ~ 26 DEG C；
（5）Feed is fed, monthly 2 times, earthworm bed is first turned over before feeding, using heap block upslide method, thickness is 10 centimetres, should not be by bed surface
Gai Man, do not ask smooth, to separate earthworm；
（6）Harvesting, harvest 4~6 times within 1 year, 40~50 days summers harvested once, and spring, 60~80 days autumns harvest once, every time
Earthworm bed substrate is supplemented after harvesting, and adjusts cultivation density, kind earthworm will update once every year, and culturing bed changes once every year.
Sulfurous method filter mud refers to that cane sugar factory is carried out during sugaring using lime and sulfur dioxide as fining agent
Filter mud caused by cane juice clarification processing.
Compound biological bacterium is made up of dissolving phosphor and dissolving potassium bacterium, geotrichum candidum, photosynthetic bacteria and saccharomycete, and their weight proportion is
2.8-3:0.6-1.4:2.2-2.8:1.2-1.8, it is put into by corn flour 10-18%, peptone 2-6%, calcium carbonate 2-6%, sucrose 6-
14% and surplus for pure water culture medium in cultivate, fermented 8-12 days at 23-28 DEG C.
Biology enzyme is polyphenol oxidase, contains cellulase, pectase and neutral proteinase.
Step（5）In, the feed fed is that mass ratio is 8:5:2 fowl and animal excrement, agricultural crop straw, corn starch sugar dreg
The compound obtained after everfermentation.
Agricultural crop straw is maize straw, wheat stalk, rice straw and soybean stalk by weight 1：1：1:1 mixing.
Fowl and animal excrement is cow dung, horsehit, pig manure and chicken manure by weight 1：1：1:1 mixing.
Corn starch sugar dreg is that cornstarch filters in sugar industry or sugaring workshop section of fermentation enterprise after double-enzyme method sugar making
The filter residue that liquid glucose obtains.
Contrast test：
Four mu of ground are chosen, embodiment 1-4 breeding earthworm method and thick using most field mounds in the market is respectively adopted
Put the mode grown in a suitable place to breed and raise earthworm, tested with " big flat No. 3 " earthworm（It is using Japan cultivars flat greatly No. 2 and north to put down No. 3 earthworms greatly
Star 2 is hybridized and formed through seed selection of purificating and rejuvenating for many years, and big flat No. 3 have following characteristics：Appearance equals No. 2 phases with big substantially
Imitative, color and luster is scarlet, and average individual weighs 0.4 gram, and maximum individual is up to 1.5 grams）, launched with every mu of 300kg amount, entered in aforementioned manners
Row raising 60 days, following table is two groups of correction datas：
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Control group | |
| Initial weight（kg） | 300 | 300 | 300 | 300 | 300 |
| Weight after 60 days（kg） | 2568 | 2775 | 2517 | 2826 | 1539 |
| Weightening ratio | 8.56 | 9.25 | 8.39 | 9.42 | 5.13 |
| Sick rate（‰） | 1.02 | 0.91 | 1.08 | 0.87 | 7.83 |
The embodiment of the present application 1-4 earthworm culture method finally obtains yield and improved than common cultured output as seen from the above table
Similar one times, and be not easy to fall ill, the earthworm of output is bright in colour, activity is high.
Each 2kg of earthworm in Example 1-4 and control group respectively, measures the nutriment composition in its dry weight body, under
Table is correction data：
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Control group | |
| Protein content（%） | 60.1 | 62.4 | 59.8 | 63.5 | 51.8 |
| Fat content（%） | 8.2 | 7.6 | 7.9 | 7.2 | 9.8 |
| Carbohydrate content（%） | 13.7 | 15.2 | 14.1 | 15.5 | 11.2 |
| VB, VD content（mg/kg） | 28.1 | 29.3 | 28.5 | 29.8 | 21.7 |
| Zinc lead chromium-copper contents of heavy metal elements（μg/g） | 58 | 57 | 57 | 57 | 207 |
The embodiment of the present application 1-4 earthworm culture method finally obtains yield than the nutriture value commonly cultivated as seen from the above table
Value is higher, and contents of heavy metal elements is low, has higher economic value.
Claims (10)
- A kind of 1. method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent, it is characterised in that it is wrapped Include following steps：（1）1-2 kilograms of compound biological bacterium is added by every cube of sulfurous method filter mud and is sufficiently mixed, then heap chevron pile into strips Body, per turning in 5-6 days 1 time, turning 2-5 times, fermentation temperature is controlled at 50-60 DEG C, is fermented 20-40 days, obtains the filter mud that ferments；Treat After centipede space temperature drops to normal temperature, then take biological agent and fermentation filter mud in mass ratio 1:100 sprinklings, earthworm bed is obtained after well mixed Matrix；（2）It is vermiculture feed lot that low yield, which abandons waste field, in transformation, and weeding is carried out to place, rolls leveling, surrounding digs good row Ditch, line-spacing is 40~45cm between earthworm bed, line-spacing center excavate it is wide, deep be 25~30cm soil box, earthworm bedside 130~ 150cm, high 35~40cm, upper strata are covered using agricultural crop straw, and a height of 20~40cm steps are laid by lower floor（1）Obtained earthworm bed Matrix；（3）Launch commodity earthworm seedling or kind earthworm seedling, injected volume are controlled in every mu of 300~400kg；（4）Daily management：Summer earthworm bed substrate thickness is maintained at 35~40cm, carry out sunshade and diligent watering work with adjust temperature, Humidity, 1~2 day in dusk watering 1 time；Winter earthworm bed substrate thickness is maintained at 30~40cm, waters once within 5~10 days, 20~ Add new earthworm bed substrate 1 time within 30 days；Controlling environment temperature on ordinary days, earthworm bed substrate water content is 60 ~ 75% at 18 ~ 26 DEG C；（5）Feed is fed, monthly 2 times, earthworm bed is first turned over before feeding, using heap block upslide method, thickness is 10 centimetres, should not be by bed surface Gai Man, do not ask smooth, to separate earthworm；（6）Harvesting, harvest 4~6 times within 1 year, 40~50 days summers harvested once, and spring, 60~80 days autumns harvest once, every time Earthworm bed substrate is supplemented after harvesting, and adjusts cultivation density, kind earthworm will update once every year, and culturing bed changes once every year.
- 2. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 1, its It is characterised by, the sulfurous method filter mud refers to that cane sugar factory uses lime and sulfur dioxide as fining agent during sugaring Carry out filter mud caused by cane juice clarification processing.
- 3. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 1, its It is characterised by, the height of the centipede space is 70-100 centimetres.
- 4. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 1, its It is characterised by, the compound biological bacterium is made up of dissolving phosphor and dissolving potassium bacterium, geotrichum candidum, photosynthetic bacteria and saccharomycete, and their weight is matched somebody with somebody Than for 2.8-3:0.6-1.4:2.2-2.8:1.2-1.8, it is put into by corn flour 10-18%, peptone 2-6%, calcium carbonate 2-6%, sugarcane Sugared 6-14% and surplus are fermented 8-12 days to be cultivated in the culture medium of pure water at 23-28 DEG C.
- 5. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 1, its Be characterised by, the biological agent is Fructus Chaenomelis ferment or polyphenol oxidase, polyphenol oxidase take containing cellulase, pectase, One or more in neutral proteinase.
- 6. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 5, its It is characterised by, the raw pawpaw of Fructus Chaenomelis ferment preparation method is smashed, and is added 5-8 times of water, is added raw pawpaw percentage by weight 2%- 3% salt and 3-5% rice vinegar, insert terrine and pickle 10-15 days, be filtrated to get Fructus Chaenomelis ferment.
- 7. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 1, its It is characterised by, step（5）In, the feed fed is that mass ratio is 8:5:2 fowl and animal excrement, agricultural crop straw, corn starch sugar The compound that slag obtains after everfermentation.
- 8. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 7, its It is characterised by, the agricultural crop straw is that the one or more in maize straw, wheat stalk, rice straw or soybean stalk are mixed Close.
- 9. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 7, its It is characterised by, the fowl and animal excrement is one or more mixing in cow dung, horsehit, pig manure and chicken manure.
- 10. the method for handling sulfurous method filter mud breeding earthworm using biological bacteria and biological agent according to claim 7, its It is characterised by, the corn starch sugar dreg is that cornstarch refines sugar workshop section after double-enzyme method sugar making in sugar industry or fermentation enterprise The filter residue that filter liquid glucose obtains.
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