CN107267558A - 用于制造发酵产物的方法 - Google Patents

用于制造发酵产物的方法 Download PDF

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Publication number
CN107267558A
CN107267558A CN201710458195.8A CN201710458195A CN107267558A CN 107267558 A CN107267558 A CN 107267558A CN 201710458195 A CN201710458195 A CN 201710458195A CN 107267558 A CN107267558 A CN 107267558A
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Prior art keywords
ala
amylase
leu
gly
alpha
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Chinese (zh)
Inventor
R·戴因哈默
J·克雷格
松井知子
高木忍
S·克拉克
J·马修斯
A·G·尤尔曼德
C-L·宋
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Novozymes AS
Novozymes North America Inc
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Novozymes AS
Novozymes North America Inc
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Publication of CN107267558A publication Critical patent/CN107267558A/zh
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    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
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    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
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    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
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    • C12P7/14Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
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    • C12R2001/85Saccharomyces
    • C12R2001/865Saccharomyces cerevisiae
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CN201710458195.8A 2011-12-02 2012-11-30 用于制造发酵产物的方法 Pending CN107267558A (zh)

Applications Claiming Priority (3)

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US201161566281P 2011-12-02 2011-12-02
US61/566,281 2011-12-02
CN201280068841.5A CN104245942A (zh) 2011-12-02 2012-11-30 用于制造发酵产物的方法

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EP (1) EP2785847B1 (enExample)
CN (2) CN104245942A (enExample)
CA (1) CA2857963C (enExample)
DK (1) DK2785847T3 (enExample)
EA (1) EA201491087A1 (enExample)
ES (1) ES2644727T3 (enExample)
IN (1) IN2014CN04905A (enExample)
MX (1) MX348696B (enExample)
WO (1) WO2013082486A1 (enExample)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103608460B (zh) 2010-12-22 2017-08-29 诺维信北美公司 用于产生发酵产物的工艺
EP2859110B1 (en) 2012-03-30 2022-10-26 Novozymes North America, Inc. Processes of producing fermentation products
US11939552B2 (en) 2013-06-24 2024-03-26 Novozymes A/S Process of recovering oil
US10093882B2 (en) * 2013-06-24 2018-10-09 Novozymes A/S Processes for recovering oil from fermentation product processes and processes for producing fermentation products
WO2015066667A1 (en) * 2013-11-04 2015-05-07 Danisco Us Inc. Proteases in wheat processing
WO2016196202A1 (en) 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
WO2017015329A1 (en) 2015-07-23 2017-01-26 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
MX392571B (es) * 2015-12-22 2025-03-24 Novozymes As Procesos para producir productos de fermentación.
EP3526336A1 (en) 2016-10-17 2019-08-21 Novozymes A/S Methods of reducing foam during ethanol fermentation
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
WO2018098124A1 (en) 2016-11-23 2018-05-31 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CA3064042A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
BR112020006356A2 (pt) * 2017-10-04 2020-09-29 Novozymes A/S polipeptídeo com atividade de protease, polinucleotídeo, construto de ácido nucleico, ou vetor de expressão recombinante, célula hospedeira recombinante, método para a produção de um polipeptídeo com atividade de protease, processos para liquefazer material contendo amido, para produzir produtos de fermentação a partir de material contendo amido e para recuperação de óleo de uma produção de produto de fermentação, composição enzimática, e, uso de uma protease s8a de palaeococcus ferrophilus.
ES2954431T3 (es) 2017-12-08 2023-11-22 Novozymes As Variantes de alfa-amilasa y polinucleótidos que codifican las mismas
US11326153B2 (en) 2017-12-08 2022-05-10 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
MX2020007914A (es) 2018-01-29 2020-10-28 Novozymes As Microorganismos con uso de nitrogeno mejorado para produccion de etanol.
WO2019161227A1 (en) 2018-02-15 2019-08-22 Novozymes A/S Improved yeast for ethanol production
CA3143381A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
PE20251535A1 (es) 2022-05-14 2025-06-05 Novozymes As Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
CN120380158A (zh) 2022-12-19 2025-07-25 诺维信公司 用于降低发酵产物生产方法后端的浆体粘度的方法
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025071409A1 (en) * 2023-09-26 2025-04-03 Mauri Technology B.V. Bakery product with reduced fodmap content
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1657614A (zh) * 1997-06-10 2005-08-24 宝生物工程株式会社 表达超热稳定蛋白的系统
CN101115843A (zh) * 2005-02-07 2008-01-30 诺维信北美公司 发酵产品产生方法
CN100564534C (zh) * 2002-02-08 2009-12-02 金克克国际有限公司 以碳底物生产乙醇的方法
CN101939421A (zh) * 2008-02-04 2011-01-05 丹尼斯科美国公司 嗜热脂肪芽孢杆菌α-淀粉酶的变体及其用途
WO2011127802A1 (en) * 2010-04-14 2011-10-20 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
US4560651A (en) 1981-04-20 1985-12-24 Novo Industri A/S Debranching enzyme product, preparation and use thereof
NO840200L (no) 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
US4536477A (en) 1983-08-17 1985-08-20 Cpc International Inc. Thermostable glucoamylase and method for its production
US4587215A (en) 1984-06-25 1986-05-06 Uop Inc. Highly thermostable amyloglucosidase
US4628031A (en) 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
JPS62126989A (ja) 1985-11-26 1987-06-09 Godo Shiyusei Kk コルテイシウム属担子菌の生産する酵素を用いた澱粉質の無蒸煮糖化法
US5162210A (en) 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
JPH05508997A (ja) 1990-08-01 1993-12-16 ノボ ノルディスク アクティーゼルスカブ 新規熱安定性プルラナーゼ
DK81293D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
ATE210181T1 (de) * 1994-06-13 2001-12-15 Takara Shuzo Co Gen für hyperthermostabile protease
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
EP0808363B1 (en) 1995-02-03 2009-05-27 Novozymes A/S A method of designing alpha-amylase mutants with predetermined properties
US6261822B1 (en) * 1995-12-12 2001-07-17 Takara Shuzo Co., Ltd. Ultrathermostable protease genes
ES2432519T3 (es) 1996-04-30 2013-12-04 Novozymes A/S Mutantes de alfa-amilasa
KR20010015754A (ko) 1997-10-13 2001-02-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 α-아밀라제 변이체
CN1261567C (zh) 1997-11-26 2006-06-28 诺维信公司 热稳定的葡糖淀粉酶
WO1999043794A1 (en) 1998-02-27 1999-09-02 Novo Nordisk A/S Maltogenic alpha-amylase variants
CA2331340A1 (en) 1998-07-15 2000-01-27 Novozymes A/S Glucoamylase variants
ES2618608T3 (es) 1999-03-30 2017-06-21 Novozymes A/S Variantes de alfa-amilasa
EP2009098A1 (en) 1999-07-09 2008-12-31 Novozymes A/S Glucoamylase variant
WO2001051620A2 (en) 2000-01-12 2001-07-19 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
CN101423824B (zh) 2000-06-02 2013-01-30 诺维信公司 角质酶变体
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
CA2778199A1 (en) 2000-08-01 2002-02-07 Novozymes A/S Alpha-amylase mutants with altered properties
AR032392A1 (es) * 2001-01-19 2003-11-05 Solvay Pharm Gmbh Mezcla de enzimas, preparado farmaceutico y utilizacion de dicho preparado.
BR0214653B1 (pt) 2001-12-07 2015-01-06 Novozymes As Construção de ácido nucléico, vetor de expressão recombinante, célula de microorganismo hospedeiro recombinante, método para produzir um polipeptídeo, uso de pelo menos uma protease, método para melhorar o valor nutricional de uma ração animal, aditivo para ração animal, composição de ração animal, e, método para o tratamento de proteínas egetais”.
ES2340390T3 (es) 2003-10-28 2010-06-02 Novozymes North America, Inc. Enzimas hibridas.
EP1682655B1 (en) 2003-10-30 2009-09-02 Novozymes A/S Carbohydrate-binding modules
CN1984998A (zh) * 2004-07-13 2007-06-20 诺维信北美公司 液化方法
DK1831384T3 (en) 2004-12-22 2015-11-23 Novozymes As Polypeptides with glucoamylase activity and polynucleotides encoding them.
DE102005062984A1 (de) * 2005-12-28 2007-07-05 Henkel Kgaa Wasch- oder Reinigungsmittel mit spezieller Amylase
CN101443447A (zh) 2006-04-19 2009-05-27 诺维信北美公司 具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸
WO2007134207A2 (en) 2006-05-12 2007-11-22 Novozymes North America, Inc. Use of a thermococcales-derived alpha-amylase for starch liquefaction or saccharification
MX2010004014A (es) * 2007-10-18 2010-05-05 Danisco Us Inc Mezclas enzimaticas para fermentacion.
WO2010008841A2 (en) 2008-06-23 2010-01-21 Novozymes A/S Processes for producing fermentation products
WO2011066576A1 (en) 2009-11-30 2011-06-03 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
MX2012006176A (es) 2009-12-01 2012-06-25 Novozymes North America Inc Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos.
CN102753680B (zh) * 2009-12-11 2015-05-13 诺维信公司 蛋白酶变体
WO2011076123A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Compositions comprising boosting polypeptide and starch degrading enzyme and uses thereof
US20120270267A1 (en) * 2010-01-04 2012-10-25 Novozymes A/S Alpha-Amylases
CN103608460B (zh) * 2010-12-22 2017-08-29 诺维信北美公司 用于产生发酵产物的工艺

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1657614A (zh) * 1997-06-10 2005-08-24 宝生物工程株式会社 表达超热稳定蛋白的系统
CN100564534C (zh) * 2002-02-08 2009-12-02 金克克国际有限公司 以碳底物生产乙醇的方法
CN101115843A (zh) * 2005-02-07 2008-01-30 诺维信北美公司 发酵产品产生方法
CN101939421A (zh) * 2008-02-04 2011-01-05 丹尼斯科美国公司 嗜热脂肪芽孢杆菌α-淀粉酶的变体及其用途
WO2011127802A1 (en) * 2010-04-14 2011-10-20 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same

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