CN107198771B - 微载体悬浮培养细胞生产猪伪狂犬gE基因缺失病毒疫苗的方法 - Google Patents
微载体悬浮培养细胞生产猪伪狂犬gE基因缺失病毒疫苗的方法 Download PDFInfo
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- CN107198771B CN107198771B CN201710317050.6A CN201710317050A CN107198771B CN 107198771 B CN107198771 B CN 107198771B CN 201710317050 A CN201710317050 A CN 201710317050A CN 107198771 B CN107198771 B CN 107198771B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0684—Cells of the urinary tract or kidneys
- C12N5/0686—Kidney cells
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- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5252—Virus inactivated (killed)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
- A61K2039/575—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 humoral response
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- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
- C12N2710/16734—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
组分(重量) | A组 | B组 | C组 | D组 |
壳聚糖(g) | 200 | 200 | 200 | 200 |
聚羟基脂肪酸酯(g) | 60 | 70 | 80 | / |
组别 | 干球直径(μm) | 湿球直径(μm) | 干球孔径(μm) | 湿球孔径(μm) |
A组 | 800~1200 | 1000~1300 | 60~90 | 70~100 |
B组 | 800~1200 | 1000~1300 | 55~85 | 60~90 |
C组 | 800~1200 | 1000~1300 | 55~90 | 60~95 |
D组 | 700~1000 | 800~1100 | 50~85 | 55~90 |
组别 | 孔隙率(%) | 吸水率(g/g) | 比表面积(cm<sup>2</sup>/g) |
A组 | 92.5 | 6.2 | 3900~4200 |
B组 | 91.2 | 6.2 | 3900~4200 |
C组 | 90.6 | 6.0 | 3900~4200 |
D组 | 91.8 | 5.3 | 3600~4000 |
Claims (7)
Priority Applications (1)
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CN201710317050.6A CN107198771B (zh) | 2017-05-08 | 2017-05-08 | 微载体悬浮培养细胞生产猪伪狂犬gE基因缺失病毒疫苗的方法 |
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CN201710317050.6A CN107198771B (zh) | 2017-05-08 | 2017-05-08 | 微载体悬浮培养细胞生产猪伪狂犬gE基因缺失病毒疫苗的方法 |
Publications (2)
Publication Number | Publication Date |
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CN107198771A CN107198771A (zh) | 2017-09-26 |
CN107198771B true CN107198771B (zh) | 2021-02-09 |
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CN201710317050.6A Active CN107198771B (zh) | 2017-05-08 | 2017-05-08 | 微载体悬浮培养细胞生产猪伪狂犬gE基因缺失病毒疫苗的方法 |
Country Status (1)
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113423825A (zh) * | 2019-02-15 | 2021-09-21 | 科学生物技术公司 | 病毒生产方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102552899A (zh) * | 2012-03-07 | 2012-07-11 | 长春百克生物科技股份公司 | 应用生物反应器灌流培养制备狂犬疫苗的方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100389193C (zh) * | 2006-01-12 | 2008-05-21 | 上海交通大学 | 安全连续封闭式细胞培养、病毒生产和灭活的方法 |
CN101036785A (zh) * | 2007-04-29 | 2007-09-19 | 中国人民解放军第二军医大学 | 肝炎疫苗的壳聚糖纳米粒口服制剂 |
CN101768206B (zh) * | 2008-12-31 | 2013-05-15 | 华北制药集团新药研究开发有限责任公司 | 一种重组人血清白蛋白的纯化方法及其应用 |
CN101875917B (zh) * | 2009-04-28 | 2012-07-04 | 北京清大天一科技有限公司 | 生物反应器微载体培养动物细胞生产猪瘟疫苗的方法 |
CN102038946A (zh) * | 2010-09-15 | 2011-05-04 | 武汉中博生物股份有限公司 | 应用生物反应器工业化生产伪狂犬病疫苗的方法 |
CN102329728B (zh) * | 2011-10-11 | 2013-12-25 | 上海理工大学 | 一种壳聚糖/rgd三维多孔微载体及其制备方法和应用 |
CN102558601B (zh) * | 2012-01-04 | 2013-09-25 | 上海理工大学 | 一种高强度多孔壳聚糖微载体的制备方法 |
CN102558581B (zh) * | 2012-01-04 | 2014-04-16 | 上海理工大学 | 一种高强度实心壳聚糖微载体的制备方法 |
CN104524563A (zh) * | 2014-12-12 | 2015-04-22 | 普莱柯生物工程股份有限公司 | 疫苗组合物及其制备方法和应用 |
CN105727277A (zh) * | 2014-12-12 | 2016-07-06 | 普莱柯生物工程股份有限公司 | 一种制备猪伪狂犬病病毒疫苗的方法及疫苗制品 |
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CN102552899A (zh) * | 2012-03-07 | 2012-07-11 | 长春百克生物科技股份公司 | 应用生物反应器灌流培养制备狂犬疫苗的方法 |
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Inventor after: Zhang Yujin Inventor after: Yan Tikun Inventor after: Huang Shufen Inventor after: Ao Yanhua Inventor before: Yan Tikun Inventor before: Zhang Yujin Inventor before: Huang Shufen Inventor before: Ao Yanhua |
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