CN107006326A - A kind of method that Denmark rose of Sharon elite plant strain is efficiently bred - Google Patents
A kind of method that Denmark rose of Sharon elite plant strain is efficiently bred Download PDFInfo
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- CN107006326A CN107006326A CN201710281593.7A CN201710281593A CN107006326A CN 107006326 A CN107006326 A CN 107006326A CN 201710281593 A CN201710281593 A CN 201710281593A CN 107006326 A CN107006326 A CN 107006326A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a kind of method that Denmark rose of Sharon elite plant strain is efficiently bred, comprise the following steps：Materials, the pretreatment of explant, the induction processing of explant adventitious root, the field planting of explant, the seedling management of explant.The advantage of the invention is that：First, convenient material drawing, material enriches, regeneration plant enough keeps the original merit of maternal plant；Second, simple to operate, reproduction speed is fast, and the species and concentration of liquid are promoted while strictly optimizing and controlling to take root；Third, species and the concentration selection of disinfectant, nutrition fluid infusion and nutrient matrix etc. also strictly with during Denmark rose of Sharon plant strain growth needed for essential elements be engaged, it is to avoid the introducing of harmful element；Fourth, explant is placed in Mixed nutrient medium in directly being taken root in warmhouse booth, forming seedling through one step culture, it is to avoid the influence and limitation of the natural cause such as season, region；Fifth, cost is low, and it is simple to operate, it is easy to grasp, expanding propagation, the plant modification suitable for the Denmark rose of Sharon can be achieved.
Description
Technical field
Field, more particularly to a kind of method that Denmark rose of Sharon elite plant strain is efficiently bred are bred the present invention relates to the rose of Sharon.
Background technology
The rose of Sharon is Malvaceae Hibiscus perennial plant, and because its florescence is longer, pattern is various, and strong adaptability, cultivation pipe
Reason is easier to, and has cultivation in China north and south various regions.
The Denmark rose of Sharon is as the improvement new varieties of the rose of Sharon, and not only leaf color is dark green, and pattern enriches, and red, orange, yellow, powder is presented, white
Etc. color；And the florescence is long, bud is more, and per basin up to more than 30 buds, single florescence is relatively about 3-5 days, as long as Post flowering is protected
The sufficient nutrient growth of card can be bloomed in the anniversary；In addition, Denmark's rose of Sharon disease resistance ability is stronger, indoor and outdoor can be cultivated, tool
There is high ornamental value.
In recent years, with the introduction and the raising of ornamental value of the Denmark rose of Sharon, Denmark's rose of Sharon demand is also being continued to increase；
Denmark's rose of Sharon fine-variety breeding and its supporting efficient the raising technology technical bottleneck and urgently as Denmark's rose of Sharon industry development
The technical problem of solution.
At present, the breeding of the Denmark rose of Sharon from foreign countries mainly by introducing seedling, and cost is higher；The country is not yet realized at present
The autonomous of Denmark's rose of Sharon seedling is bred.These seriously limit the development of Denmark's rose of Sharon industry.
The problems of therefore, bred for the current Denmark rose of Sharon, it is badly in need of a kind of simple, efficient Denmark rose of Sharon of exploitation
Fast breeding technique is a kind of method that Denmark rose of Sharon elite plant strain is efficiently bred.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of reproduction speed is fast, simple to operate, cost
It is relatively low, and the method that Denmark's rose of Sharon elite plant strain of maternal strain good characteristic can be kept efficiently to breed.
The present invention is achieved by the following technical solutions：A kind of method that Denmark rose of Sharon elite plant strain is efficiently bred, bag
Include following steps：
(1) draw materials
Choose the blade with petiole and the stem section with blade of the potted plant Denmark rose of Sharon in greenhouse with excellent economical character
It is used as explant；Wherein, the blade method of drawing material with petiole is：Lower band petiole is broken from the junction of Denmark's rose of Sharon stem section and petiole
Blade；Stem section method of drawing material with blade is：Stem section is cut into scissors a length of with 1 axillary bud and 1 leaf
1.0-3.0cm micro- stem section；
(2) pretreatment of explant
Blade of the Denmark rose of Sharon with petiole in step (1) and the stem section with blade are placed under flowing water and are rinsed, so
Surface sterilization is carried out by liquor potassic permanganate afterwards, dried in the shade after pulling out, standby；
(3) the induction processing of explant adventitious root
The morphology lower end of explant in step (2) after disinfecting is placed in take root promotion liquid in carry out it is indefinite
The induction processing of root；Wherein take root and promote liquid to be specially containing 300-1000mg/L methyl α-naphthyl acetates and 100-500mg/L spermidines
Take root promotion liquid；
(4) field planting of explant
By blade of the Denmark rose of Sharon with petiole after promotion liquid processing of taking root in step (3) and the stem section with blade
Morphology inferior end notch insertion nutrient matrix disk in, be defined by non-overlapping copies, and spray the nutrition fluid infusion without organic element；
(5) the seedling management of explant
Blade of the Denmark rose of Sharon with petiole after being colonized in step (4) and the stem section with blade are covered into plastic foil, Yu Wen
Continue the induction and seedling culture of adventitious root in the greenhouse of room；During culture, the battalion for being once free of organic element was sprayed every 1-3 weeks
Support fluid infusion.
One of preferred embodiment as the present invention, excellent economical character refers to high yield, Hua great, flower in the step (1)
One or more of character in phase length, good stress resistance.
One of preferred embodiment as the present invention, the potted plant Denmark rose of Sharon is specially to be introduced from Denmark in the step (1)
The potted plant Denmark rose of Sharon.
As one of preferred embodiment of the present invention, blade of the Denmark rose of Sharon with petiole and with blade in the step (2)
Stem section under flowing water in rinsing 10-20min.
One of preferred embodiment as the present invention, liquor potassic permanganate is concentration 0.5-1.0 ‰ height in the step (2)
Potassium manganate antiseptic solution, is sterilized in use, sample to be sterilized is placed in concentration 0.5-1.0 ‰ potassium permanganate antiseptic solution
5-15min.
One of preferred embodiment as the present invention, by under the morphology of the explant after disinfecting in the step (3)
End is placed in promotion liquid of taking root and handles 10-90s.
As one of preferred embodiment of the present invention, by the blade with petiole and the stem section with blade in the step (4)
In morphology inferior end notch insertion nutrient matrix, its intubating length is 0.5-1.0cm.
One of preferred embodiment as the present invention, nutrient matrix disk is that mass ratio is 1 in the step (4):3 Nutrition Soil
With the mixture of river sand.
One of preferred embodiment as the present invention, step (5) medium temperature chamber greenhouse is 20-26 DEG C of temperature, relative humidity
80-85% warmhouse booth.
One of preferred embodiment as the present invention, the nutrition fluid infusion without organic element is specially to be free of organic element
1/2MS basic culture solutions.
The advantage of the present invention compared with prior art is：First, the explant used in the present invention is Denmark's rose of Sharon band petiole
Blade and micro- stem section with blade, with convenient material drawing, material enriches, and the plant regenerated can keep maternal plant former
The advantages such as some merits；The promotion immersion bubble stem section 10-90s second, present invention use is taken root, 14-21d have been obtained with
Whole Denmark's rose of Sharon regeneration plant, with simple to operate, the advantage such as reproduction speed is fast, while strictly optimizing and controlling promotion of taking root
The species and concentration of liquid, become the optium concentration of Denmark's rose of Sharon root induction；Third, the pretreatment of the present invention, field planting
And species and the concentration selection of disinfectant, nutrition fluid infusion and the nutrient matrix in seedling management process etc. also strictly with Denmark's wood
Essential elements needed for during rose of Sharon plant strain growth is engaged, while avoiding the introducing of harmful element；Fourth, present invention employs
Explant is placed in Mixed nutrient medium in directly being taken root in warmhouse booth, forming seedling through one step culture, it is to avoid season, region etc. are natural
The influence and limitation of factor；Fifth, not only production regeneration seedling cost is low by the present invention, and operating method is simple, it is easy to grasp；
It can realize simultaneously to regenerate blade of the seedling with petiole and stem section as the further expanding propagation of explant progress, plant can be made
Quantity realizes geometry multiplication by stages in short period of time, the plant modification suitable for the Denmark rose of Sharon.
Brief description of the drawings
Fig. 1 is the Denmark rose of Sharon for the method that a kind of Denmark rose of Sharon elite plant strain in embodiment 1 is efficiently bred with petiole
Blade is taken root figure；
Fig. 2 is the Denmark rose of Sharon for the method that a kind of Denmark rose of Sharon elite plant strain in embodiment 1 is efficiently bred with petiole
Blade seedling figure；
Fig. 3 is the Denmark rose of Sharon for the method that a kind of Denmark rose of Sharon elite plant strain in embodiment 2 is efficiently bred with blade
Micro- stem section is taken root figure；
Fig. 4 is the Denmark rose of Sharon for the method that a kind of Denmark rose of Sharon elite plant strain in embodiment 2 is efficiently bred with blade
Micro- stem section seedling figure；
Fig. 5 is the Denmark's rose of Sharon regrowth for the method that a kind of Denmark rose of Sharon elite plant strain in embodiment 2 is efficiently bred
Field transplanting figure.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementations
Example.
Embodiment 1
The method that a kind of Denmark rose of Sharon elite plant strain of the present embodiment is efficiently bred, comprises the following steps：
(1) draw materials
Choose in greenhouse from Denmark's introduction with excellent economical character (with high yield, Hua great, florescence length, good stress resistance
Middle one or more of character) the blade with petiole of the potted plant Denmark rose of Sharon be used as explant；Wherein, the blade with petiole takes
Material method is：The blade of lower band petiole is broken from the junction of Denmark's rose of Sharon stem section and petiole；
(2) pretreatment of explant
Blade of the Denmark rose of Sharon with petiole in step (1) is placed under flowing water and is rinsed 10min, then passes through concentration
0.5 ‰ potassium permanganate antiseptic solution carries out surface sterilization 5min, is dried in the shade after pulling out, standby；
(3) the induction processing of explant adventitious root
The morphology lower end of explant in step (2) after disinfecting is placed in 10s in promotion liquid of taking root, carried out
The induction processing of adventitious root；Promotion liquid of wherein taking root is specially the rush of taking root containing 300mg/L methyl α-naphthyl acetates and 100mg/L spermidines
Feed liquor；
(4) field planting of explant
The morphology lower end of blade of the Denmark rose of Sharon with petiole after promotion liquid processing of taking root in step (3) is cut
(mass ratio is 1 to mouth insertion nutrient matrix disk:3 Nutrition Soil and the mixture of river sand) in, it is defined by non-overlapping copies, intubating length
0.5cm, and spray the 1/2MS basic culture solutions without organic element；
(5) the seedling management of explant
By plastic foil in bucket cover of the Denmark rose of Sharon with petiole after being colonized in step (4), in 20 DEG C of temperature, relative humidity
Continue the induction and seedling culture of adventitious root in 80% warmhouse booth；During culture, sprayed every 1 week and be once free of organic member
The nutrition fluid infusion of element；Cultivate after 7d, petiole base expands to form milky root restriction；14d is cultivated, petiole base formation is about
The healthy and strong adventitious root of 2.0cm milky；21d is cultivated, the adventitious root of petiole base is about 4.0cm (such as Fig. 1), and the basic companion of petiole
There is axillary bud sprouting, ultimately form complete Denmark rose of Sharon plant (such as Fig. 2)；The rooting rate of blade of the Denmark rose of Sharon with petiole is up to
94.3%, planting percent is up to 91.5%.
Embodiment 2
The method that a kind of Denmark rose of Sharon elite plant strain of the present embodiment is efficiently bred, comprises the following steps：
(1) draw materials
Choose in greenhouse from Denmark's introduction with excellent economical character (with high yield, Hua great, florescence length, good stress resistance
Middle one or more of character) the stem section with blade of the potted plant Denmark rose of Sharon be used as explant；Wherein, the stem section with blade takes
Material method is：Stem section is cut into micro- stem section of a length of 1.0-3.0cm with 1 axillary bud and 1 leaf with scissors；
(2) pretreatment of explant
Stem section of the Denmark rose of Sharon with blade in step (1) is placed under flowing water and is rinsed 15min, then passes through concentration
0.75 ‰ potassium permanganate antiseptic solution carries out surface sterilization 10min, is dried in the shade after pulling out, standby；
(3) the induction processing of explant adventitious root
The morphology lower end of explant in step (2) after disinfecting is placed in 10-90s in promotion liquid of taking root,
Carry out the induction processing of adventitious root；Promotion liquid of wherein taking root is specially the life containing 800mg/L methyl α-naphthyl acetates and 300mg/L spermidines
Root promotes liquid；
(4) field planting of explant
The morphology lower end of stem section of the Denmark rose of Sharon with blade after promotion liquid processing of taking root in step (3) is cut
(mass ratio is 1 to mouth insertion nutrient matrix disk:3 Nutrition Soil and the mixture of river sand) in, it is defined by non-overlapping copies, intubating length
0.8cm, and spray the 1/2MS basic culture solutions without organic element；
(5) the seedling management of explant
Stem section of the Denmark rose of Sharon with blade after being colonized in step (4) is covered into plastic foil, in 23 DEG C of temperature, relative humidity
Continue the induction and seedling culture of adventitious root in 83% warmhouse booth；During culture, sprayed every 2 weeks and be once free of organic member
The nutrition fluid infusion of element；Cultivate after 7d, micro- stem segment with axillary buds is sprouted, and the morphology lower end of micro- stem section is expanded and with milky
Former base is formed；14d is cultivated, the morphology lower end of micro- stem section forms the adventitious root (such as Fig. 3) for being about 1.5cm；Cultivate after 21d, it is micro-
The inductivity of the adventitious root of stem section is up to 100%, and planting percent is up to 100% (such as Fig. 4)；Denmark's rose of Sharon fast propagating seedling warp after seedling
The acclimation for spending 1 week, can directly be transplanted in crop field (such as Fig. 5), field transplanting rate is up to 100%.
Embodiment 3
The method that a kind of Denmark rose of Sharon elite plant strain of the present embodiment is efficiently bred, comprises the following steps：
(1) draw materials
Choose in greenhouse from Denmark's introduction with excellent economical character ((with high yield, Hua great, florescence length, good stress resistance
Middle one or more of character) the potted plant Denmark rose of Sharon the blade with petiole and the stem section with blade be used as explant；Wherein,
Blade method of drawing material with petiole is：The blade of lower band petiole is broken from the junction of Denmark's rose of Sharon stem section and petiole；With blade
Stem section method of drawing material is：Stem section is cut into micro- stem section of a length of 1.0-3.0cm with 1 axillary bud and 1 leaf with scissors；
(2) pretreatment of explant
Blade of the Denmark rose of Sharon with petiole in step (1) and the stem section with blade are placed under flowing water and are rinsed
20min, then carries out surface sterilization 15min by the potassium permanganate antiseptic solution of concentration 1.0 ‰, is dried in the shade after pulling out, standby；
(3) the induction processing of explant adventitious root
The morphology lower end of explant in step (2) after disinfecting is placed in 90s in promotion liquid of taking root, carried out
The induction processing of adventitious root；Wherein take root and promote liquid to be specially taking root containing 1000mg/L methyl α-naphthyl acetates and 500mg/L spermidines
Promote liquid；
(4) field planting of explant
By blade of the Denmark rose of Sharon with petiole after promotion liquid processing of taking root in step (3) and the stem section with blade
Morphology inferior end notch insertion nutrient matrix disk (mass ratio is 1:3 Nutrition Soil and the mixture of river sand) in, not weigh mutually
Standard, intubating length 1.0cm are stacked as, and sprays the 1/2MS basic culture solutions without organic element；
(5) the seedling management of explant
Blade of the Denmark rose of Sharon with petiole after being colonized in step (4) and the stem section with blade are covered into plastic foil, Yu Wen
Continue the induction and seedling culture of adventitious root in 26 DEG C of degree, the warmhouse booth of relative humidity 85%；During culture, sprayed every 3 weeks
Apply the nutrition fluid infusion for being once free of organic element；Cultivate 7d, petiole and micro- stem section base portion formation root restriction, and the axillary bud of stem section
Start to sprout；Cultivate 14d, petiole and micro- stem section healthy and strong adventitious root of base portion formation milky；Cultivate 21d, petiole base with
Axillary bud sprouting, the blade with petiole and micro- stem section with blade ultimately form complete Denmark rose of Sharon plant；Wherein with petiole
The rooting rate of blade is 89.7%, and planting percent is 83.4%；The rooting rate of micro- stem section is 94.6%, and planting percent is 94.6%.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of method that Denmark rose of Sharon elite plant strain is efficiently bred, it is characterised in that comprise the following steps：
(1) draw materials
Choose the blade with petiole of the potted plant Denmark rose of Sharon in greenhouse with excellent economical character and the stem section conduct with blade
Explant；Wherein, the blade method of drawing material with petiole is：The leaf of lower band petiole is broken from the junction of Denmark's rose of Sharon stem section and petiole
Piece；Stem section method of drawing material with blade is：Stem section is cut into a length of 1.0- with 1 axillary bud and 1 leaf with scissors
3.0cm micro- stem section；
(2) pretreatment of explant
Blade of the Denmark rose of Sharon with petiole in step (1) and the stem section with blade are placed under flowing water and are rinsed, Ran Houtong
Cross liquor potassic permanganate and carry out surface sterilization, dried in the shade after pulling out, standby；
(3) the induction processing of explant adventitious root
The morphology lower end of explant in step (2) after disinfecting is placed in take root and promotes to carry out adventitious root in liquid
Induction is handled；Wherein take root and promote liquid to be specially taking root containing 300-1000mg/L methyl α-naphthyl acetates and 100-500mg/L spermidines
Promote liquid；
(4) field planting of explant
By blade of the Denmark rose of Sharon with petiole after promotion liquid processing of taking root in step (3) and the shape of the stem section with blade
In state inferior end notch insertion nutrient matrix disk, it is defined by non-overlapping copies, and spray the nutrition fluid infusion without organic element；
(5) the seedling management of explant
Blade of the Denmark rose of Sharon with petiole after being colonized in step (4) and the stem section with blade are covered into plastic foil, it is big in greenhouse
Continue the induction and seedling culture of adventitious root in canopy；During culture, the once nutrition without organic element was sprayed every 1-3 weeks and is mended
Liquid.
2. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(1) excellent economical character refers to character one or more of in high yield, Hua great, florescence length, good stress resistance in.
3. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(1) the potted plant Denmark rose of Sharon is specially the potted plant Denmark rose of Sharon introduced from Denmark in.
4. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(2) blade of the Denmark rose of Sharon with petiole and the stem section with blade under flowing water in rinsing 10-20min in.
5. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(2) liquor potassic permanganate is concentration 0.5-1.0 ‰ potassium permanganate antiseptic solution in, in use, sample to be sterilized is placed in
5-15min is sterilized in concentration 0.5-1.0 ‰ potassium permanganate antiseptic solution.
6. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(3) the morphology lower end of the explant after disinfecting is placed in promotion liquid of taking root in and handles 10-90s.
7. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(4) blade with petiole and the morphology inferior end notch of the stem section with blade are inserted in nutrient matrix in, its intubating length is
0.5-1.0cm。
8. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(4) nutrient matrix disk is that mass ratio is 1 in:3 Nutrition Soil and the mixture of river sand.
9. the method that Denmark rose of Sharon elite plant strain according to claim 1 is efficiently bred, it is characterised in that the step
(5) medium temperature chamber's greenhouse is 20-26 DEG C of temperature, relative humidity 80-85% warmhouse booth.
10. the method efficiently bred according to any described Denmark's rose of Sharon elite plant strains of claim 1-9, it is characterised in that institute
It is specially the 1/2MS basic culture solutions without organic element to state the nutrition fluid infusion without organic element.
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| CN112931217A (en) * | 2021-04-08 | 2021-06-11 | 中国科学院合肥物质科学研究院 | Tissue culture regeneration method of Denmark shrubalthea |
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| CN109315289A (en) * | 2018-10-09 | 2019-02-12 | 云南善源生物科技发展有限公司 | Hymsleya amabilis Novel sterilizing mode |
| CN112931217A (en) * | 2021-04-08 | 2021-06-11 | 中国科学院合肥物质科学研究院 | Tissue culture regeneration method of Denmark shrubalthea |
| CN112931217B (en) * | 2021-04-08 | 2022-04-22 | 中国科学院合肥物质科学研究院 | Tissue culture regeneration method of Denmark shrubalthea |
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