CN109315289A - Hymsleya amabilis Novel sterilizing mode - Google Patents
Hymsleya amabilis Novel sterilizing mode Download PDFInfo
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- CN109315289A CN109315289A CN201811172208.6A CN201811172208A CN109315289A CN 109315289 A CN109315289 A CN 109315289A CN 201811172208 A CN201811172208 A CN 201811172208A CN 109315289 A CN109315289 A CN 109315289A
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- Prior art keywords
- hymsleya amabilis
- stem section
- thimerosal
- hymsleya
- amabilis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of hymsleya amabilis Novel sterilizing mode, carried out disinfection processing using liquor potassic permanganate to hymsleya amabilis, comprising the following steps: pretreatment, hymsleya amabilis spray stem section is intercepted as outer finger body, branch is cut into the stem section that length is 5cm, extra blade is removed, is put into spare in vial；Thimerosal is prepared, potassium permanganate and sterile water are mixed, is deployed into the thimerosal that concentration is 0.1-0.2%；It impregnates, the thimerosal that b is completed pours into the vial equipped with hymsleya amabilis stem section, renews medicining liquid dipping after every immersion 0.5-1.5h, and total immersion is steeped 3-5 times.The advantages of the present invention over the prior art are that: by original hymsleya amabilis disinfection way for needing to sterilize after water planting is bred, it is improved to directly intercept the disinfection way that hymsleya amabilis spray stem section uses liquor potassic permanganate, substantially reduce the cultivation cycle for obtaining sterile hymsleya amabilis stem section, and potassium permanganate is small to plant injury and bactericidal effect is good, expands propagating and cultivating for subsequent hymsleya amabilis and improves good basis.
Description
Technical field
The present invention relates to hymsleya amabilis Cultivating techniques field, in particular to hymsleya amabilis Novel sterilizing modes.
Background technique
Existing hymsleya amabilis tissue cultures, mostly expand using the stem section with leaf bud it is numerous, growth cycle be 45-60 days.It is avenging
The case where easily generating callus in gallbladder tissue culture procedures, seedling separation is caused not sprout or stem section are directly dead, therefore,
Disinfection and sterilizing before expanding numerous culture are the important links of entire tissue culture procedures, and it is subsequent whether disinfection is thoroughly directly related to
The progress of work.Existing disinfection way means are single, such as adopt annotinous branch as explant, water planting carried out indoors, wait sprout
Hair sends forth branches, and carries out water planting as explant, is rinsed well branch with flowing water, is cut into 5-6 centimeter length stem segment with axillary bud,
2-5S is impregnated with 75% alcohol, then handles 5min, the time-consuming inconvenience of the method with 0.1% mercuric chloride, and effect is unobvious, causes
Hymsleya amabilis expands propagating and cultivating can not large-scale production.Therefore, the hymsleya amabilis Novel sterilizing side that one kind is easy to operate, works well is designed
Formula.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the above technological deficiencies, provide a kind of hymsleya amabilis Novel sterilizing mode.
In order to solve the above technical problems, technical solution provided by the invention are as follows: a kind of hymsleya amabilis Novel sterilizing mode uses height
Potassium manganate solution carries out disinfection processing to hymsleya amabilis, comprising the following steps:
A. pre-process: branch is cut into the stem section that length is 5cm, removed by interception hymsleya amabilis spray stem section as outer finger body
Extra blade is put into spare in vial；
B. it prepares thimerosal: potassium permanganate and sterile water is mixed, be deployed into the thimerosal that concentration is 0.1-0.2%；
C. impregnate: the thimerosal that b is completed pours into the vial equipped with hymsleya amabilis stem section, after every immersion 0.5-1.5h
Renew medicining liquid dipping, total immersion is steeped 3-5 times.
Preferably, the hymsleya amabilis stem section by step c immersion treatment is not needed using rinsed with sterile water.
Preferably, by step a-c, treated that hymsleya amabilis stem section is put into MS culture medium carries out expansion propagating and cultivating.
Preferably, 2,4D of the MS+6BA and 3mg/L in the MS culture medium added with 2mg/L.
The advantages of the present invention over the prior art are that: need the hymsleya amabilis sterilized after water planting is bred to disappear for original
Malicious mode is improved to directly intercept the disinfection way that hymsleya amabilis spray stem section uses liquor potassic permanganate, substantially reduces acquisition nothing
The cultivation cycle of bacterium hymsleya amabilis stem section, and potassium permanganate is small to plant injury and bactericidal effect is good, inoculation survival rate reaches 85%
Left and right, hymsleya amabilis stem section after treatment are put into the MS culture medium of 2, the 4D of MS+6BA and 3mg/L added with 2mg/L, will be former
The numerous ratio of expansion of 1:3-1:4 is improved to the numerous ratio of expansion of 1:10-1:15.
Specific embodiment
The present invention is described in further detail below.
A kind of hymsleya amabilis Novel sterilizing mode carries out disinfection processing to hymsleya amabilis using liquor potassic permanganate, comprising the following steps:
A. pre-process: branch is cut into the stem section that length is 5cm, removed by interception hymsleya amabilis spray stem section as outer finger body
Extra blade is put into spare in vial；
B. it prepares thimerosal: potassium permanganate and sterile water is mixed, be deployed into the thimerosal that concentration is 0.1-0.2%；
C. impregnate: the thimerosal that b is completed pours into the vial equipped with hymsleya amabilis stem section, after every immersion 0.5-1.5h
Renew medicining liquid dipping, total immersion is steeped 3-5 times.
Hymsleya amabilis stem section by step c immersion treatment is not needed using rinsed with sterile water, by step a-c treated snow
Gallbladder stem section is put into MS culture medium and carries out expansion propagating and cultivating, 2 of MS+6BA and 3mg/L added with 2mg/L in the MS culture medium,
4D。
Case study on implementation one
A kind of hymsleya amabilis Novel sterilizing mode carries out disinfection processing to hymsleya amabilis using liquor potassic permanganate, comprising the following steps:
A. pre-process: branch is cut into the stem section that length is 5cm, removed by interception hymsleya amabilis spray stem section as outer finger body
Extra blade is put into spare in vial；
B. it prepares thimerosal: potassium permanganate and sterile water is mixed, be deployed into the thimerosal that concentration is 0.1%；
C. impregnate: the thimerosal that b is completed pours into the vial equipped with hymsleya amabilis stem section, renews after every immersion 1.5h
Medicining liquid dipping, total immersion are steeped 3 times.
Hymsleya amabilis stem section by step c immersion treatment is not needed using rinsed with sterile water, by step a-c treated snow
Gallbladder stem section is put into MS culture medium and carries out expansion propagating and cultivating, and MS culture medium is at present using most common culture medium, nothing with higher
Machine salinity, mineral nutrition needed for capable of guaranteeing tissue growth can also accelerate the growth of callus, the MS culture medium
The 2,4D (dichlorphenoxyacetic acid) of interior MS+6BA (6-benzyladenine) and 3mg/L added with 2mg/L.
Case study on implementation two
A kind of hymsleya amabilis Novel sterilizing mode carries out disinfection processing to hymsleya amabilis using liquor potassic permanganate, comprising the following steps:
A. pre-process: branch is cut into the stem section that length is 5cm, removed by interception hymsleya amabilis spray stem section as outer finger body
Extra blade is put into spare in vial；
B. it prepares thimerosal: potassium permanganate and sterile water is mixed, be deployed into the thimerosal that concentration is 0.2%；
C. impregnate: the thimerosal that b is completed pours into the vial equipped with hymsleya amabilis stem section, renews after every immersion 0.5h
Medicining liquid dipping, total immersion are steeped 3 times.
Hymsleya amabilis stem section by step c immersion treatment is not needed using rinsed with sterile water, by step a-c treated snow
Gallbladder stem section is put into MS culture medium and carries out expansion propagating and cultivating, and MS culture medium is at present using most common culture medium, nothing with higher
Machine salinity, mineral nutrition needed for capable of guaranteeing tissue growth can also accelerate the growth of callus, the MS culture medium
The 2,4D (dichlorphenoxyacetic acid) of interior MS+6BA (6-benzyladenine) and 3mg/L added with 2mg/L.
Case study on implementation three
A kind of hymsleya amabilis Novel sterilizing mode carries out disinfection processing to hymsleya amabilis using liquor potassic permanganate, comprising the following steps:
A. pre-process: branch is cut into the stem section that length is 5cm, removed by interception hymsleya amabilis spray stem section as outer finger body
Extra blade is put into spare in vial；
B. it prepares thimerosal: potassium permanganate and sterile water is mixed, be deployed into the thimerosal that concentration is 0.15%；
C. impregnate: the thimerosal that b is completed pours into the vial equipped with hymsleya amabilis stem section, renews after every immersion 1h and disappears
Venom impregnates, and total immersion is steeped 4 times.
Hymsleya amabilis stem section by step c immersion treatment is not needed using rinsed with sterile water, by step a-c treated snow
Gallbladder stem section is put into MS culture medium and carries out expansion propagating and cultivating, and MS culture medium is at present using most common culture medium, nothing with higher
Machine salinity, mineral nutrition needed for capable of guaranteeing tissue growth can also accelerate the growth of callus, the MS culture medium
The 2,4D (dichlorphenoxyacetic acid) of interior MS+6BA (6-benzyladenine) and 3mg/L added with 2mg/L.
The above description is only a preferred embodiment of the patent of the present invention, is not intended to limit the invention patent, all at this
Made any modifications, equivalent replacements, and improvements etc., should be included in the invention patent within the spirit and principle of patent of invention
Protection scope within.
Claims (4)
1. a kind of hymsleya amabilis Novel sterilizing mode, which is characterized in that it is carried out disinfection processing using liquor potassic permanganate to hymsleya amabilis, including
Following steps:
A. pre-process: branch is cut into the stem section that length is 5cm, it is extra to remove as outer finger body by interception hymsleya amabilis spray stem section
Blade is put into spare in vial；
B. it prepares thimerosal: potassium permanganate and sterile water is mixed, be deployed into the thimerosal that concentration is 0.1-0.2%；
C. impregnate: the thimerosal that b is completed pours into the vial equipped with hymsleya amabilis stem section, renews after every immersion 0.5-1.5h
Medicining liquid dipping, total immersion are steeped 3-5 times.
2. hymsleya amabilis Novel sterilizing mode according to claim 1, it is characterised in that: by the hymsleya amabilis of step c immersion treatment
Stem section is not needed using rinsed with sterile water.
3. hymsleya amabilis Novel sterilizing mode according to claim 1, it is characterised in that: by step a-c treated hymsleya amabilis
Stem section is put into MS culture medium and carries out expansion propagating and cultivating.
4. hymsleya amabilis Novel sterilizing mode according to claim 2, it is characterised in that: be added in the MS culture medium
The 2,4D of the MS+6BA and 3mg/L of 2mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811172208.6A CN109315289A (en) | 2018-10-09 | 2018-10-09 | Hymsleya amabilis Novel sterilizing mode |
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| CN201811172208.6A CN109315289A (en) | 2018-10-09 | 2018-10-09 | Hymsleya amabilis Novel sterilizing mode |
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| CN109315289A true CN109315289A (en) | 2019-02-12 |
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| CN201811172208.6A Pending CN109315289A (en) | 2018-10-09 | 2018-10-09 | Hymsleya amabilis Novel sterilizing mode |
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Citations (10)
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| CN107041309A (en) * | 2017-04-26 | 2017-08-15 | 中国科学院合肥物质科学研究院 | A kind of method of fast breeding Chinese tallow tree plant |
| CN107646689A (en) * | 2017-11-07 | 2018-02-02 | 临沧惠康中药材资源开发有限公司 | The method that a kind of tissue cultures of hymsleya amabilis and later stage breed |
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Application publication date: 20190212 |
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