CN106978440A - 一种外源基因导入红腺忍冬的方法 - Google Patents
一种外源基因导入红腺忍冬的方法 Download PDFInfo
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- CN106978440A CN106978440A CN201710253760.7A CN201710253760A CN106978440A CN 106978440 A CN106978440 A CN 106978440A CN 201710253760 A CN201710253760 A CN 201710253760A CN 106978440 A CN106978440 A CN 106978440A
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- seedling
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- lonicera hypoglauca
- hypoglauca miq
- infected
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109349104A (zh) * | 2018-10-09 | 2019-02-19 | 广西壮族自治区药用植物园 | 从外植体快速获得红腺忍冬组培生根苗的方法 |
Citations (4)
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CN103320469A (zh) * | 2013-07-08 | 2013-09-25 | 山东大学 | 一种对金银花不定芽转化获得转基因植株的方法 |
US20130266608A1 (en) * | 2012-03-22 | 2013-10-10 | Pharma Green Llc | Methods and compositions to produce vaccines against smallpox in plants |
CN104170733A (zh) * | 2014-08-29 | 2014-12-03 | 广西壮族自治区药用植物园 | 红腺忍冬无菌组培苗叶柄愈伤组织不定根诱导的方法 |
CN105409778A (zh) * | 2015-12-22 | 2016-03-23 | 广西壮族自治区药用植物园 | 红腺忍冬无菌组培苗叶柄不定芽诱导的方法 |
-
2017
- 2017-04-18 CN CN201710253760.7A patent/CN106978440B/zh active Active
Patent Citations (4)
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US20130266608A1 (en) * | 2012-03-22 | 2013-10-10 | Pharma Green Llc | Methods and compositions to produce vaccines against smallpox in plants |
CN103320469A (zh) * | 2013-07-08 | 2013-09-25 | 山东大学 | 一种对金银花不定芽转化获得转基因植株的方法 |
CN104170733A (zh) * | 2014-08-29 | 2014-12-03 | 广西壮族自治区药用植物园 | 红腺忍冬无菌组培苗叶柄愈伤组织不定根诱导的方法 |
CN105409778A (zh) * | 2015-12-22 | 2016-03-23 | 广西壮族自治区药用植物园 | 红腺忍冬无菌组培苗叶柄不定芽诱导的方法 |
Non-Patent Citations (8)
Title |
---|
FENGHUA SHI ET AL.: "Application of the phosphomannose-isomerase/mannose selection system in the Agrobacterium-mediated transformation of Lonicera hypoglauca Miq.", 《JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY》 * |
刘国花等: "根癌农杆菌介导灰毡毛忍冬遗传转化体系的建立", 《中药材》 * |
刘良玉: "酵母磷酸甘露糖异构酶基因作为选择标记在转基因水稻中的研究", 《华中农业大学2007届本科生毕业"论文设计"创新作品选编 上》 * |
师凤华等: "广西地方标准《红腺忍冬组培快繁技术规程》(征求意见稿)编制说明", 《原创力文档》 * |
李建军等: "忍冬种子休眠特性及破眠方法研究", 《河南农业科学》 * |
李静等: "山银花繁殖技术研究进展", 《广西农学报》 * |
谭木秀等: "红腺忍冬种子沙培萌发条件和种子沙培苗红腺忍冬种子沙培萌发条件和种子沙培苗灭菌方法的初步研究", 《中药材》 * |
黄语梦等: "山银花的组织培养研究进展", 《广西中医药大学学报》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109349104A (zh) * | 2018-10-09 | 2019-02-19 | 广西壮族自治区药用植物园 | 从外植体快速获得红腺忍冬组培生根苗的方法 |
CN109349104B (zh) * | 2018-10-09 | 2021-07-06 | 广西壮族自治区药用植物园 | 从外植体快速获得红腺忍冬组培生根苗的方法 |
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Application publication date: 20170725 Assignee: Bobai Dayu agriculture and animal husbandry Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980045130 Denomination of invention: A Method of Introducing Exogenous Genes into Lonicera japonica Granted publication date: 20210518 License type: Common License Record date: 20231101 |
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