CN106676163A - 检测毕赤酵母细胞dna的引物及方法 - Google Patents
检测毕赤酵母细胞dna的引物及方法 Download PDFInfo
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- CN106676163A CN106676163A CN201510757729.8A CN201510757729A CN106676163A CN 106676163 A CN106676163 A CN 106676163A CN 201510757729 A CN201510757729 A CN 201510757729A CN 106676163 A CN106676163 A CN 106676163A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- Proteomics, Peptides & Aminoacids (AREA)
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- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
DNA | R2 | slope | E |
毕赤酵母+H2O | 0.999 | -3.31 | 100.5% |
毕赤酵母+CHO | 0.998 | -3.25 | 103.1% |
毕赤酵母+Vero | 0.998 | -3.17 | 106.8% |
毕赤酵母+人 | 0.998 | -3.38 | 97.6% |
Claims (10)
Priority Applications (1)
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CN201510757729.8A CN106676163B (zh) | 2015-11-09 | 2015-11-09 | 检测毕赤酵母细胞dna的引物及方法 |
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CN201510757729.8A CN106676163B (zh) | 2015-11-09 | 2015-11-09 | 检测毕赤酵母细胞dna的引物及方法 |
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CN106676163A true CN106676163A (zh) | 2017-05-17 |
CN106676163B CN106676163B (zh) | 2020-12-18 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111334607A (zh) * | 2020-05-21 | 2020-06-26 | 天津欧德莱生物医药科技有限公司 | 一种定量Pichia pastoris细胞残留的核酸检测试剂盒 |
-
2015
- 2015-11-09 CN CN201510757729.8A patent/CN106676163B/zh active Active
Non-Patent Citations (3)
Title |
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CRAVEIRO RB等: "High expression of human carboxypeptidase M in Pichia pastoris: purification and partial characterization", 《BRAZ J MED BIOL RES》 * |
LÕOKE M等: "Extraction of genomic DNA from yeasts for PCR-based applications", 《BIOTECHNIQUES》 * |
刘晶晶等: "荧光定量PCR检测重组新蛭素中毕赤酵母基因组DNA的残留量", 《生物技术通讯》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111334607A (zh) * | 2020-05-21 | 2020-06-26 | 天津欧德莱生物医药科技有限公司 | 一种定量Pichia pastoris细胞残留的核酸检测试剂盒 |
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CN106676163B (zh) | 2020-12-18 |
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Inventor after: Wang Tao Inventor after: Liang Chenggang Inventor after: Yang Zhixing Inventor after: Wu Wanxin Inventor after: Zong Weiying Inventor after: Lv Ping Inventor after: Zhang Hui Inventor after: Li Jing Inventor before: Yang Zhixing Inventor before: Wu Wanxin Inventor before: Zong Weiying Inventor before: Wang Tao Inventor before: Wen Ming Inventor before: Zhu Bingmei Inventor before: Zhang Le |
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Effective date of registration: 20180214 Address after: 313000 No. 1366 Hongfeng Road, Zhejiang, Huzhou Applicant after: HUZHOU R & D CENTER FOR NUTRITION AND HEALTH, SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCE Applicant after: NATIONAL INSTITUTES FOR FOOD AND DRUG CONTROL Applicant after: HUZHOU SHENKE BIOLOGICAL TECHNOLOGY Co.,Ltd. Address before: 5, 313000 floor, Huzhou South Taihu science and technology innovation center, 1366 Hongfeng Road, Zhejiang, Huzhou Applicant before: HUZHOU R & D CENTER FOR NUTRITION AND HEALTH, SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCE Applicant before: Huzhou Shenke Biological Technology Co.,Ltd. |
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Address after: 313000 Hongfeng Road, Huzhou, Zhejiang 1366 Patentee after: Huzhou Zhongke nutrition and health innovation center Patentee after: NATIONAL INSTITUTES FOR FOOD AND DRUG CONTROL Patentee after: Huzhou Shenke Biotechnology Co.,Ltd. Address before: 313000 Hongfeng Road, Huzhou, Zhejiang 1366 Patentee before: HUZHOU R & D CENTER FOR NUTRITION AND HEALTH, SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCE Patentee before: NATIONAL INSTITUTES FOR FOOD AND DRUG CONTROL Patentee before: HUZHOU SHENKE BIOLOGICAL TECHNOLOGY Co.,Ltd. |