CN106472370A - Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method - Google Patents
Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method Download PDFInfo
- Publication number
- CN106472370A CN106472370A CN201610873807.5A CN201610873807A CN106472370A CN 106472370 A CN106472370 A CN 106472370A CN 201610873807 A CN201610873807 A CN 201610873807A CN 106472370 A CN106472370 A CN 106472370A
- Authority
- CN
- China
- Prior art keywords
- tricyclazole
- fermentation liquid
- rice
- ctenopharyngodon idellus
- deinsectization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method, comprise the following steps：（1）Base arranges：Deep ploughing soil, depth be 40～60cm, and implement base manure, every mu apply the farmyard manure that becomes thoroughly decomposed, become thoroughly decomposed dung polluted water；（2）Nursery：Based on neat before seedling is moistened with shallow water, shallow water frequent irrigation after emerging；Every mu of mixture spraying antagonistic bacterium fermentation liquid and tricyclazole, is watered 45kg thin water fast spraying, downgrades rice shoot；（3）The prevention and control of plant diseases, pest control：Before 200 Ctenopharyngodon idellus are placed on rice seedling bed, wrap up the rice shoot root of long-grained nonglutinous rice combination by cover, the mixture using antagonistic bacterium fermentation liquid and tricyclazole sprays to blade face.In the process of nursery, rice shoot carried out prevent rice blast by the mixture of antibacterium fermentation liquid and tricyclazole, and Ctenopharyngodon idellus can eat the worm's ovum in rice field, reaches commensalism, is effectively utilized worm's ovum.And the disinsection efficiency by antibacterium fermentation liquid and the mixture of tricyclazole is very strong, and persistency is strong.
Description
Technical field
The invention belongs to the implantation methods technical field of Oryza sativa L., particularly to a kind of Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultivation side
Method.
Background technology
And rice blast is one of important disease of Oryza sativa L. now, this disease can cause the rice yield significantly underproduction, sternly
The underproduction 40% during weight, or even total crop failure, plantation disease-resistant variety is the preventing and treating most economical effective measures of this disease at present.But in production
Disease-resistant variety is less, and many disease-resistant varieties just lose resistance in popularizing planting after 3～5 years.
Provide a kind of rice cultivating method for solving above-mentioned technical problem Chinese patent CN105850623, including making
Treat transplanting seedlings；Turn over fertilizing management；Transplant plantation；The steps such as land for growing field crops fertilizing management；The method respectively at four leaf stage and is fallen
Two leaf stage according to 200mL/ mu consumption to foliage-spray liquid silicon potassium；In front 1 leaf age of the critical Leaf-Age-Period of effective tillering, when
When stem tiller number reaches the 85% of expected spike number, start draining and put field, jointing carries out wetting irrigation for paddy field, alternation of wetting and drying to ripe.This cultivation
Culture method can reduce super hybridization rice rice blast and occur, and reach anti-banded sclerotial blight and blast resisting target.
But above-mentioned patent still suffers from following technological deficiency:Worm's ovum absorbs field nutrition, can be in row after being killed by medicine
Discharge from field during water, cause certain waste.
Content of the invention
The invention is intended to providing a kind of Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method with durable broad spectrum blast resisting, can
Worm's ovum is carried out effectively utilizes, it is to avoid cause to waste.
Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method in this programme, comprises the following steps：
(1) base arranges：Deep ploughing soil, depth is 40～60cm, and implements base manure, applies the farmyard manure that becomes thoroughly decomposed, becomes thoroughly decomposed for every mu
Dung polluted water；
(2) nursery：Based on neat before seedling is moistened with shallow water, shallow water frequent irrigation after emerging；Every mu spray antagonistic bacterium fermentation liquid and
The mixture of tricyclazole, is watered 45kg thin water fast spraying, downgrades rice shoot；
(3) prevention and control of plant diseases, pest control：The rice shoot root of long-grained nonglutinous rice combination before 200 Ctenopharyngodon idellus are placed on rice seedling bed, is wrapped up by cover
Portion, the mixture using antagonistic bacterium fermentation liquid and tricyclazole sprays to blade face.
The principle of this programme and having the beneficial effects that：This programme needs base is arranged in advance, the base after arrangement
On to apply the farmyard manure that becomes thoroughly decomposed, become thoroughly decomposed dung polluted water, and can give rice nutrition to Ctenopharyngodon idellus for food, and the depth in the soil of deep ploughing
Degree, in 40～60cm, is to ensure that Oryza sativa L. can fully obtain nutrition, in the process of nursery, by antibacterium fermentation liquid and
The sprinkling of the mixture of tricyclazole carries out to rice shoot preventing rice blast to process, and Ctenopharyngodon idellus can eat the worm's ovum in rice field, reaches altogether
Raw state, is effectively utilized the worm's ovum being killed, it is to avoid wasting phenomenon occurs.And antibacterium fermentation liquid and the mixing of tricyclazole
The disinsection efficiency of thing is very strong, and persistency is strong.
Further, the mixture in step (3), is made up of the raw material of following weight portion：Antagonistic bacterium fermentation liquid 20-60
Part, tricyclazole 15-40 part.
Further, the mixture in step (3), is made up of the raw material of following weight portion：20 parts of antagonistic bacterium fermentation liquid, three
15 parts of ring azoles.
Further, the mixture in step (3), is made up of the raw material of following weight portion：60 parts of antagonistic bacterium fermentation liquid, three
40 parts of ring azoles.
Further, the mixture in step (3), is made up of the raw material of following weight portion：40 parts of antagonistic bacterium fermentation liquid, three
25 parts of ring azoles.
Further, the mixture in step (3), is made up of the raw material of following weight portion：30 parts of antagonistic bacterium fermentation liquid, three
30 parts of ring azoles；Wherein the effective dose of antagonistic bacterium and the mass ratio of tricyclazole are 1:2.
Specific embodiment
With reference to embodiment, the invention will be further described：
(1) base arranges
A, rice seedling bed select and foster and apply fertilizer
Rice seedling bed should select the field that physical features is higher, Soil structure is good, after rice seedling bed is chosen, entered before the winter primary cultivation first, deep ploughing
Soil, depth requirements are in 40～60cm；Secondly, implement base manure, every mu apply the farmyard manure that becomes thoroughly decomposed, become thoroughly decomposed dung polluted water, after fertilizer is applied
Whole good rice seedling bed, sowing before by rice seedling bed weed and loosen the soil flat, do furrow, then divide furrow, wait to sow；
B, land for growing field crops prepare：First to soil, total Se content is measured, and then intensive cultivation is carefully whole.
(2) nursery
A, seed pre-sowing treatment：Combined as seed rice from the stronger Anhui rice hybridization medium rice of high-quality, high yield, resistance,
Bask seeds before broadcasting 1-2 days, then carry out Medicament soaked seed, concrete grammar is：First seed is soaked 8 hours with clear water, pull out dry in the air 2 little
When, adding 0.002g sodium selenite, after being sufficiently mixed, soak seed 5kg, general immersion 30 hours is eluriated dry with clear water after seed soaking
Only accelerating germination after；
B, sowing:In the first tenday period of a month in May, germinateing of handling well is waited to sow son by every mu of field standard of dry kind of 12.5kg, point furrow
It is seeded in the rice seedling bed handled well, sowing post legged paddy enters mud；
C, seedling management：Based on neat before seedling is moistened with shallow water, shallow water frequent irrigation after emerging；Every mu sprays antagonistic bacterium fermentation liquid
And tricyclazole, it is watered 45kg thin water fast spraying, downgrade rice shoot.
(3) prevention and control of plant diseases, pest control
Before 200 Ctenopharyngodon idellus are placed on rice seedling bed, the rice shoot root that long-grained nonglutinous rice is combined is wrapped up using cover, in case careless
Fish gnaws the root of rice shoot, and Ctenopharyngodon idellus are natural in rice field to be gone to eat the worm's ovum in rice seedling bed, to reach pest control, and due to seedling
Seedling is to hybridize out using the stronger Anhui rice 161 of resistance, therefore, can produce antagonistic bacterium, and antagonistic bacterium has and persistently resists
Insect pest, and pairing is mixed with tricyclazole, the weight portion of its pairing is as follows：
Table 1
| Implement 1 | Implement 2 | Implement 3 | |
| Tricyclazole | 20 parts | 40 parts | 60 parts |
| Antagonistic bacterium | 15 parts | 25 parts | 40 parts |
In order to confirm to contain antagonistic bacterium in the seed rice that long-grained nonglutinous rice combines, inventor studies further to this, has done following reality
Test：
Experiment material：
(1) 384 individual plants of Anhui rice, its this 384 individual plant makes spore suspension, and this spore suspension is inoculated in PDA and puts down
Add 5ml sterilized water in plate, in the Pyricularia oryzae of 28 DEG C of constant temperature culture 5d, scrape mycelia, be made into hyphal suspension.
(2) hyphal suspension carries out separating, and using plate dilution method, hyphal suspension separator is on NA flat board in 28 DEG C
It is inverted culture 2～3d, after being fermented, choose the good bacterial strain of antagonistic effect, put in NA test tube,.
Experimentation：
(1) concentration of tricyclazole is respectively 100,500,1000,2000,3000,4000,5000 μ g/mL.Now by rice blast
Bacterium spore suspension uniformly sprays after rice leaf 24h, sprays the tricyclazole of antagonistic bacterium fermentation liquid and above-mentioned variable concentrations
Mutually mix, and control the effective dose of antagonistic bacterium and the mass ratio of tricyclazole effective dose to be：1:0、1:1、1:2、2:1、0:
1.With spray clear water for comparison, magnaporthe grisea spore suspension after 24h, often process 3 repetitions, often repeat 1 basin, every 10 plants of water of basin
Rice, is spraying antagonistic bacterium fermentation liquid and tricyclazole mitigation liquid, is maintaining culture 5～7d, count the incidence of each process, such as
Shown in table 2：
As seen from Table 2, the preventive effect that antagonistic bacterium fermentation liquid is applied with tricyclazole proportioning is above 80%, wherein antagonism
Antibacterial and tricyclazole proportioning are 1:When 2, preventive effect reaches 90.33%, significant difference has been administered alone with tricyclazole, and
Tricyclazole does not have any impact to Ctenopharyngodon idellus.In table, CK is expressed as blank experiment (in addition to clear water, no other effective ingredient).
Above-described is only embodiments of the invention, and in scheme, the general knowledge here such as known concrete structure and characteristic is not made
Excessive description.It should be pointed out that for a person skilled in the art, on the premise of without departing from present configuration, acceptable
Make some deformation and improve, these also should be considered as protection scope of the present invention, these are implemented all without the impact present invention
Effect and practical applicability.This application claims protection domain should be defined by the content of its claim, in description
Specific embodiment etc. records the content that can be used for explaining claim.
Claims (6)
1. Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method is it is characterised in that comprise the following steps：
Base arranges：Deep ploughing soil, depth be 40～60cm, and implement base manure, every mu apply the farmyard manure that becomes thoroughly decomposed, become thoroughly decomposed dung polluted water；
Nursery：Based on neat before seedling is moistened with shallow water, shallow water frequent irrigation after emerging；Every mu sprays antagonistic bacterium fermentation liquid and tricyclazole
Mixture, is watered 45kg thin water fast spraying, downgrades rice shoot；
The prevention and control of plant diseases, pest control：Before 200 Ctenopharyngodon idellus are placed on rice seedling bed, wrap up the rice shoot root of long-grained nonglutinous rice combination by cover, adopt
The mixture of antagonistic bacterium fermentation liquid and tricyclazole sprays to blade face.
2. Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method according to claim 1 it is characterised in that：Step（3）In mixing
Thing, is made up of the raw material of following weight portion：Antagonistic bacterium fermentation liquid 20-60 part, tricyclazole 15-40 part.
3. Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method according to claim 1 it is characterised in that：Step（3）In mixing
Thing, is made up of the raw material of following weight portion：20 parts of antagonistic bacterium fermentation liquid, 15 parts of tricyclazole.
4. Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method according to claim 1 it is characterised in that：Step（3）In mixing
Thing, is made up of the raw material of following weight portion：60 parts of antagonistic bacterium fermentation liquid, 40 parts of tricyclazole.
5. Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method according to claim 1 it is characterised in that：Step（3）In mixing
Thing, is made up of the raw material of following weight portion：40 parts of antagonistic bacterium fermentation liquid, 25 parts of tricyclazole.
6. Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method according to claim 1 it is characterised in that：Step（3）In mixing
Thing, is made up of the raw material of following weight portion：30 parts of antagonistic bacterium fermentation liquid, 30 parts of tricyclazole；The effective dose of wherein antagonistic bacterium
Mass ratio with tricyclazole is 1:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610873807.5A CN106472370A (en) | 2016-09-30 | 2016-09-30 | Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method |
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|---|---|---|---|
| CN201610873807.5A CN106472370A (en) | 2016-09-30 | 2016-09-30 | Oryza sativa L. Ctenopharyngodon idellus symbiosis deinsectization cultural method |
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| CN106472370A true CN106472370A (en) | 2017-03-08 |
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| CN203661832U (en) * | 2013-12-31 | 2014-06-25 | 杭州广硕生态农业开发有限公司 | Paddy field soft-shelled turtle breeding ecological system |
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| CN104115713A (en) * | 2014-06-28 | 2014-10-29 | 广西壮族自治区农业科学院经济作物研究所 | Rice ridge-pulverization ecological and efficient cultivation method |
| CN203985309U (en) * | 2014-04-28 | 2014-12-10 | 三门峡雏鹰农牧有限公司 | A kind of prevent animal chew or rub hinder trees protect tree armor |
| CN104381064A (en) * | 2014-09-24 | 2015-03-04 | 江苏农林职业技术学院 | Paddy rice planting method |
-
2016
- 2016-09-30 CN CN201610873807.5A patent/CN106472370A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101683062A (en) * | 2008-09-28 | 2010-03-31 | 陈启龙 | Mixing breeding method of fish and duck in paddy field |
| CN101548635A (en) * | 2009-05-14 | 2009-10-07 | 唐开平 | Rice cropping method |
| CN101697737A (en) * | 2009-11-09 | 2010-04-28 | 江苏省农业科学院 | Bacillus subtilis and compound wettable sterilizing powder and use thereof |
| CN102268390A (en) * | 2011-07-07 | 2011-12-07 | 华中农业大学 | Bacillus amyloliquefaciens for generating fungi apoptosis lipopeptide, and preparation method and application thereof |
| CN102433282A (en) * | 2011-12-16 | 2012-05-02 | 华南农业大学 | Bacillus subtilis NB12, as well as culture method and application thereof |
| CN203661832U (en) * | 2013-12-31 | 2014-06-25 | 杭州广硕生态农业开发有限公司 | Paddy field soft-shelled turtle breeding ecological system |
| CN103907556A (en) * | 2014-03-20 | 2014-07-09 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Novel method for fish culture in rice field |
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