CN106260581A - The method being the organic multiple complex feed additive of raw material production with blackstrap - Google Patents
The method being the organic multiple complex feed additive of raw material production with blackstrap Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention discloses a kind of method being the organic multiple complex feed additive of raw material production with blackstrap, it is characterized in that: its blackstrap and trace element are after multicomponent complexation reaction, then obtain through ferment in second time with Semen Maydis powder, bean dregs, Testa oryzae and fermented bacterium；The weight portion of described blackstrap is 10 25 parts；Semen Maydis powder 5 10 parts；58 parts of bean dregs；58 parts of Testa oryzae；Fermented bacterium 0.1 1 parts；Trace element 0.1 0.3 parts；The composition of described trace element and the parts by weight of each component: 15 parts of ferrous sulfate, 14 parts of zinc sulfate, calcium oxalate 25 parts, manganese sulfate 14 parts, 0.5 1 parts of copper sulfate.The present invention uses the by-product molasses in sugar refinery to prepare feed additive through complexation and ferment in second time, belongs to the project that turns waste into wealth of leftover bits and pieces recycling, the most externally discharges waste residue, environmental protection；Trace element utilization rate is high, low cost, has fragrant and sweet taste, adds the appetite that improve cattle and sheep in feedstuff to after fermentation, and cattle and sheep are liked to eat, and growth is fast.
Description
Technical field
The present invention relates to technical field prepared by breeding feed, specifically with blackstrap for the organic multiple complexation of raw material production
The method of thing feed additive and product.
Background technology
Trace element plays an important role in animal body, for maintaining body function and optimum growh, numerous
Grow the most indispensable with immunoreation.The required trace element of animal mainly includes copper, zinc, ferrum, manganese, calcium, magnesium and cobalt etc..Lack
Trace element can cause being greatly lowered of growth of animal and production performance, directly influences the culture benefit of raiser.
In feedstuff there is larger difference in content and the titer of contained trace element, needs by feed additive form
It is added.Producing and practice is being used mostly the inorganic microelements such as copper sulfate, zinc sulfate and ferrous sulfate at present, inorganic
In use there is following defect in trace element: bioavailability is low, and animal is only capable of utilizing the inorganic micro-of addition less than 10%
Secondary element, the part not being utilized then is excluded external, not only causes waste but also increase animal body and the burden of environment；Additionally
The trace element metal ion of ionic state is affected by phytic acid, oxalic acid and cellulose in intestinal, reduces it further and absorbs
Rate；Antagonism between element also affects biological value simultaneously, and vitamin can be caused by the redox reaction between metal ion
Destroy, so that nutrient substance runs off, reduce its absorption rate.
So that the trace element of inorganic salt is easier to be absorbed, gas chromatography and trace element are carried out network by people
Close reaction, such as EDTA, aminoacid, humic acid etc., but, some Organic substance complex reaction effects are bad, and being not suitable for of having is cooked
Feedstuff.
The enhancing realized along with scientific and technological progress and health of people, poultry, while pursuing animal high yield, are also produced by people
Quality safety problem is more and more paid attention to, and the environmental pollution to using high dose inorganic substances to cause the most increasingly is paid close attention to.Therefore,
Trace element molasses complex feed additive is as the research of feed additive and is applied to for Animal nutrition area research
One of hot subject.
Sugar industry is one of the pillar industry in Guangxi, produces the blackstrap of about 1,500,000 tons every year in the sucrose course of processing.
Sweet molasses contain the sucrose of 24-36%, and other sugar account for 12-24%, colloid 3-4%, and mineral accounts for the materials such as 8-10%, be have important
The resource of value.Liquid condition molasses viscosity is high, is liable to stick to vessel surface, thus causes transport and the inconvenience used.With
Time molasses as a kind of feed additive, lower than other energy feed price, and can be fully utilized, but liquid condition molasses
Bonding is easily caused in adding procedure.Molasses solid state is mainly subtracted by the method for spray drying or heat drying at present
Few moisture therein is to reach the effect of solidification.But, there is the problem that energy consumption is bigger, and the molasses storage prepared in said method
During easily lump, limit its application.The sucrose waste molasses in Guangxi exists mainly as alcohol production raw material, the market price
500-600 yuan/ton, added value is low, and its ethanol production process environmental pollution is the most extremely serious, and a large amount of molasses face economic benefit
Low and the dual-pressure of environmental pollution.
Molasses are the side-products of sugar industry.Molasses contain a small amount of thick protein, generally 3%～6%, belong to non-protein more
Nitrogen class, such as ammonia, amide and nitrate etc., and amino-acid nitrogen only accounts for 38%～50%, and non essential amino acid such as aspartic acid,
Content of glutamic acid is more, and therefore protein biological value is relatively low, but aspartic acid and glutamic acid are delicious amino acid, therefore
Can stimulating animal appetite significantly in animal feed.Molasses, as a kind of organic energy raw material, have begun to make in feedstuff
With.
It has been investigated that, although molasses are a lot of as the research of feedstuff, but are mostly to be added directly in feedstuff by molasses
Feeding poultry, this mode can make nutrient substance be difficult to be absorbed by organisms utilization.It addition, these are invented in described feedstuff
The most do not add trace element, even if adding a small amount of trace element in single invention is also to add with inorganic ions form
, the most inevitably there is the disadvantages mentioned above of inorganic microelement in this.
Therefore, how through suitable chemical reaction, molasses and trace element metal ion are organically combined, both
Functional strong, the advantage of palatability of molasses can be played, the disadvantages mentioned above of inorganic microelement can be overcome again, be that there is positive effect
Problem.
Summary of the invention
It is an object of the invention to provide a kind of by-product blackstrap using sugar refinery is the organic multiple network of main material production
The method of compound feed additive, the method processing and fabricating is simple, low cost, use the by-product molasses in sugar refinery through complexation and
Ferment in second time prepares feedstuff to be added, and belongs to the project that turns waste into wealth of leftover bits and pieces recycling, the most externally discharges waste residue, green ring
Protect；Manufacture method is simple, low cost, has the appetite that fragrant and sweet taste, trace element utilization rate are high, improve cattle and sheep, cattle and sheep after fermentation
Love is eaten, and growth is fast, and resistance against diseases is strong.
The present invention realizes by the following technical solutions:
The method being the organic multiple complex feed additive of raw material production with blackstrap, its blackstrap and trace element are through too much
After unit's complex reaction, then obtain through ferment in second time with Semen Maydis powder, bean dregs, Testa oryzae and fermented bacterium；
The weight portion of described blackstrap is 10-25 part；Semen Maydis powder 5-10 part；Bean dregs 5-8 part；Testa oryzae 5-8 part；Fermented bacterium
0.1-1 part；Trace element 0.1-0.3 part；
The composition of described trace element and each component parts by weight: ferrous sulfate 1-5 part, zinc sulfate 1-4 part, calcium oxalate
2-5 part, manganese sulfate 1-4 part.
The weight water content of described blackstrap is 15%-25%.
The preparation process of fermented bacterium is:
(1) first cell culture medium of strain is cultivated: will be commercially available strain and cultivate 1-2 days at 22-35 DEG C with first cell culture medium respectively；
Described strain includes that candidiasis, bacillus subtilis, lactic acid bacteria, photosynthetic bacteria, whiterot fungi, Trichoderma viride, marsh are red
Pseudomonas, streptococcus faecalis and cellulase；
The raw material weight mark of described first cell culture medium is: glucose 5-8%, agar powder 5-8%, potassium dihydrogen phosphate 3-5%, chlorine
Changing sodium 2-5%, ferrous sulfate 1-3%, zinc sulfate 1-2%, herbage juice 5-10%, remaining is the mashed potato boiled；Preparation process is
The furnishing pasty state that added water by above-mentioned raw materials cooks 5-10 minute；
(2) secondary medium of strain is cultivated: after all strains one-level cultivated merge according to equivalent weight ratio, be put into two
Level inoculation of medium, cultivates 3-5 days at 22-35 DEG C, obtains fermented bacterium；
The raw material weight number of described secondary medium is: brown sugar 5-8 part, Semen Maydis powder 5-8 part, sodium chloride 2-5 part, di(2-ethylhexyl)phosphate
Hydrogen potassium 3-5 part, ferrous sulfate 1-3 part, zinc sulfate 1-2 part, copper sulfate 0.5-1 part, herbage juice 5-10 part, Testa Tritici 10-15 part, boil
Ripe mashed potato 10-20 part；Preparation process is that the furnishing pasty state that added water by above-mentioned raw materials cooks 5-10 minute；Secondary medium steams
Adding the stomach slag taken out from cattle and sheep ruminant stomach and gastric juice mix homogeneously after ripe, parts by weight are the 1-of secondary medium gross weight
3%。
With the process that blackstrap is the organic multiple complex feed additive of raw material production it is:
(1) heat in blackstrap being placed in reactor by above-mentioned parts by weight, keep temperature in the kettle between 85-105 DEG C, every time
The component adding a kind of trace element is respectively reacted 1-2 hour, and course of reaction is stirred continuously, and obtains reaction solution；
(2) adding alkali in reaction solution, regulation pH is between 6.0-7.0；Described alkali is sodium hydroxide, sodium carbonate, hydroxide
One in potassium, ammonium hydroxide or potassium carbonate.
(3) after the reaction solution natural cooling adding alkali, Semen Maydis powder, Testa oryzae and fermented bacterium mix homogeneously are added laggard
Row anaerobic fermentation is cultivated 7-15 days；Obtain premix material, seal standby；
(4) carry out anaerobic fermentation with premix material after being mixed homogeneously by bean dregs again to cultivate 7-15 days；I.e. obtain with blackstrap as raw material
Organic multiple complex feed additive.
Described herbage juice is graminous pasture or leguminous forage, including timothy grass (timothy), orchard grass (orchard
Grass), bluegrass (kentucky blue grass), thin wheat, Pennisetum hydridum, Arundo donax grass, fescue grass, Rice dumpling leaf, Herba Setariae Viridis, purple lucerne
Mu, Herba Trifolii Pratentis, Russian red clover, one or more in Caulis arachidis hypogaeae, vetch and Herba Kummerowiae Striatae, preparation process is by above-mentioned fresh
Forage breaks into juice, does not filter directly use.
Feed additive can be prepared with the method that blackstrap is the organic multiple complex feed additive of raw material production
Product.
Described Candida utilis is again Candida utilis or Candida utilis, Latin entitled Candida utilis,
The cell of Candida utilis is rounded, oval or sausage shape, and size is (3.5～4.5) um × (7～13) um；Liquid is trained
Support and do not produce mould, at the bottom of pipe, have bacterial sediment.It is edible that Candida utilis can utilize molasses, wood hydrolysis liquid etc. to produce people and animals
Protein, can regulate animal intestinal micro-ecology balance, improve feed digestibility, strengthen animal body immunity.Candida utilis ferment
Female fermented feed that cellulose raw material can be utilized to produce nutrition increasing richness, and molasses just contain substantial amounts of cellulose.Additionally, by
In Candida utilis cell rich in vitamin B and protein, moreover it is possible to provide the part nutrient substance needed for animal.As feedstuff
Multiple enzyme can be produced after the cracking of enzyme additive yeast cell wall, the yeast cells lived also can the multiple enzyme of exocytosis, such as starch
Enzyme, protease, cellulase etc..Thus, yeast can strengthen the digestibility and utilization to nutrient substance, promotes growth of animals or poultry, promotes it
Appetite, strengthens resist the disease and the ability of anti-stress.
Described bacillus subtilis, Classification system: Bacillus subtilis, is the one of bacillus.Hay
Bacillus cereus has stronger protease, amylase and lipase active, can be activated enzyme by internal activation of zymogen, also may be used
Secrete other enzymes a series of, such as pectase, glucanase, cellulase etc., can help to decompose non-starch polysaccharides(nsp) in plant feed
Deng material, improve efficiency of feed utilization, and acid intestinal environment can the intestinal peristalsis promoting of stimulating animal, facilitating digestion absorbs.
Described lactic acid bacteria (lactic acid bacteria, LAB) is that a class can utilize fermentable carbohydrate to produce
The common name of the antibacterial of raw a large amount of lactic acid.This bacterioid is extremely wide in distributed in nature, has abundant species diversity.They
It is not only research classification, biochemical, hereditary, molecular biology and engineered ideal material, there is important in theory
Art is worth, and in key areas using value also poles closely-related with human lives such as industry, farming and animal husbandry, food and medicines
High.
Described photosynthetic bacteria, English name: Photosynthetic Bacteria Abbr, (being called for short PSB) is on the earth
Occur the earliest, nature generally exists, has the prokaryote of original luminous energy synthetic system, be under anaerobic to carry out not
Put the general name of the photosynthetic antibacterial of oxygen, be the class gram negative bacteria that is formed without spore ability, be a class using light as
The energy, can utilize under anaerobism illumination or aerobic dark condition the Organic substance in nature, sulfide, ammonia etc. as hydrogen donor hold concurrently
Carbon source carries out photosynthetic microorganism.Photosynthetic bacteria is distributed widely in the soil of nature, paddy field, marsh, lake, Jiang Hai etc.
Place, is distributed mainly on the anoxic zone that in aquatic environment, light can be transmitted to.Can be from Cangzhou Zhongxin Biological Technology Co., Ltd.
Buy, address: Yao Guantun industrial park, Cang County, Cang County Hebei province, Cangzhou, Hebei, network address: http: //
czzxsw.cn.china.cn。
Described whiterot fungi, Latin literary fame: Phanerochaetc chrysosporium, it is belonging to Basidiomycotina
Fungus, gains the name because rotten timber is white, is one of the microorganism of timber main component of can degrading.Timber is in white rot process
Middle major part fiber still keeps complete, and cellulose crystallity change is little.It is therefore envisaged that utilize lignin degrading selectivity
Good whiterot fungi carries out bio-pulping, can open up the new way of pulping process.Whiterot fungi is except locating for pre-by lignin degrading
Outside reason, bio-bleaching, bio-pulping, other organic xenobiotics is also had the strongest capacity of decomposition, thus in the treatment of waste water
Also have wide practical use.Industrial Co., Ltd. can be ground from upper sea valley to buy, address: Songjiang District in Shanghai City's Xin Zhuan highway 1290
Number, network address: http://www.labbase.net/UserCompany/Default-64354.html.
Described Trichoderma viride (Trichoderma viride) is the one of trichoderma, extensive in distributed in nature, often
Saprophytic on timber, seed and plant residue.Trichoderma viride can produce multiple has bioactive enzyme system, institute's cellulase-producing
One of bacterial strain that activity is the highest, produced cellulase has Degradation to crop, and effect is the best, and Trichoderma viride is again one
Plant resourceful antagonistic microbe, plant pathology Biological control has important effect.
Described Rhodopseudomonas palustris (Rhodop seudanonas palustris) belongs to photosynthetic bacteria (English contracting
Write PSB) one, be one of microorganism the most ancient on the earth.PSB thalline is nutritious, and protein content is up to 65%, natural pond
Pool rhodopseudomonas belongs to the one of photosynthetic bacteria (english abbreviation PSB), is one of microorganism the most ancient on the earth.PSB bacterium
Body is nutritious, and protein content is up to 65%, and rich in various bioactivators, and there is strong adaptability, it is resistant to highly concentrated
The organic wastewater of degree and stronger decomposition and inversion ability, also have the features such as certain tolerance and capacity of decomposition to the poisonous substance such as phenol, cyanogen.
Described streptococcus faecalis is again enterococcus faecalis.Application C polysaccharide antigen, according to Lan Shi (Lancefield) serum credit
Class, can be divided into many groups by streptococcus.Streptococcus faecalis belongs to D group.D group streptococcus divides enterococcus and non-enterococcus two class.The former
Including streptococcus faecalis (S.faecalis), streptococcus faecalis (S.faecium) and streptococcus durans (S.durans), the latter has cattle chain
Coccus (S.bovis) and streptococcus equinus (S.equinus).Enterococcus faecalis (Enterococcus faecalis) is gram
The positive, hydrogen peroxide negative cocci, is one of flora in humans and animals intestinal, and it can produce natural antibiotics, is conducive to
Body health;The antibacterial substances such as bacteriocin, the growth of the suppression pathogen such as escherichia coli and Salmonella can also be produced simultaneously, change
Kind intestinal microenvironment;Urease-producing antibacterial and the breeding of putrefaction bacteria in intestinal can also be suppressed, reduce intestinal urease and endotoxin
Content, make ammonia and endotoxic content in blood decline.Enterococcus faecalis (E. faecalis) is as a kind of probiotic bacteria, doctor
Learn and be used widely in food engineering field.It addition, enterococcus is a quasi-microorganism of normal presence in digestive tract, stick at intestinal
Film has stronger tolerance and colonization ability, and is a kind of amphimicrobian lactic acid bacteria, with anaerobism, cultivation preservation condition harshness
Bacillus bifidus compare, be more suitable for produce and application.
Described cellulase is (English: cellulase) to be the one of enzyme, play living things catalysis when decomposition of cellulose and make
With.It is the protein that cellulose decomposition can be become oligosaccharide or monosaccharide.Cellulase is widely present in the organism of nature.
Antibacterial, fungus, cellulase can be produced in animal body etc..The cellulase being generally used for producing comes from fungus, compares allusion quotation
Type have trichoderma (Trichoderma), aspergillus (Aspergillus) and Penicillium (Penicillium).
Above-mentioned strain is bought from strain market or is cultivated by Guangxi Academy Of Sciences biology and obtains.
The microorganisms such as the Candida utilis of present invention employing, bacillus subtilis are after one-level is cultivated and two grades are cultivated
The fermented bacterium obtained, the multiple beneficial flora in fermented bacterium can draw poultry in a large number, fowl is difficult by organic nitrogen, inorganic
Nitrogen, is allowed to change into the bacterium protein that nutrition is higher, and the complex of blackstrap and trace element is played good sending out
Ferment effect, can improve absorption function and the resistance against diseases of cattle and sheep.And in secondary medium, add herbage juice and cattle and sheep ruminant stomach
The stomach slag of middle taking-up and gastric juice, herbage juice is graminous pasture or leguminous forage, including timothy grass (timothy), orchard grass
(orchard grass), bluegrass (kentucky blue grass), thin wheat, Pennisetum hydridum, Arundo donax grass, fescue grass, Rice dumpling leaf, Canis familiaris L. tail
Grass, alfalfa, Herba Trifolii Pratentis, Russian red clover, one or more in Caulis arachidis hypogaeae, vetch and Herba Kummerowiae Striatae, preparation process be by
Above-mentioned fresh forage breaks into juice, does not filter directly use.Adding herbage juice can allow microorganism preferably fit in incubation
A large amount of crude fibre, the Caulis et Folium Oryzae of lignin, Caulis arachidis hypogaeae and vegetable material environment should be there is, by crude fibre, lignin, pass through biology life
The effect changed, changes into nonabsorbable for poultry polymeric carbohydrate low point of carbohydrate of absorbable utilization；Add
Add the stomach slag taken out in cattle and sheep ruminant stomach and gastric juice can allow fermented bacterium adapt to the psalterial environment of cattle and sheep, make fermented bacterium send out
Make fermentation raw material closer to cattle and sheep ruminant stomach environment during ferment, after cattle and sheep food, be not likely to produce the situations such as gastrointestinal upset, it is possible to
Appetite strengthening, promotes gastrointestinal peristalsis and the effect of secretion, improves the good palatability of feedstuff.
Molasses are the side-products of sugar industry.Molasses contain a small amount of thick protein, generally 3%～6%, belong to non-protein more
Nitrogen class, such as ammonia, amide and nitrate etc., and amino-acid nitrogen only accounts for 38%～50%, and non essential amino acid such as aspartic acid,
Content of glutamic acid is more, and therefore protein biological value is relatively low, but aspartic acid and glutamic acid are delicious amino acid, therefore
Can stimulating animal appetite significantly in animal feed.The present invention uses blackstrap to be the organic multiple complex feedstuff of raw material production
Additive, belongs to the project that turns waste into wealth of leftover bits and pieces recycling, the most externally discharges waste residue, environmental protection；Achieve the low one-tenth of molasses
This, the comprehensive utilization of non-secondary pollution, production process does not produces " three wastes ", for the comprehensive utilization of molasses open one new
Approach, not only increases the economic benefit in sugar refinery, also provides substantial amounts of feed resource for animal husbandry, reduce feeding cost,
Sugar Industry, animal husbandry, feedstuff industry and environment sustainable development aspect are all extremely important, have good
, the brightest application prospect；To development environment-friendly type, save-grain animal husbandry, it is respectively provided with important meaning.
Feed additive of the present invention, has fine good palatability due to the addition of molasses, and trace element utilization rate is high, light
Micro-sugar sweet fragrance makes cattle and sheep like taking food, and adds the palatability strengthening feedstuff in feedstuff to, and feed additive contains growth-promoting
The long factor and profitable strain, can significantly improve daily gain, efficiency of feed utilization and body weight, simultaneously after the poultry such as cattle, sheep are edible
Production performance can also be improved, improve meat.
Detailed description of the invention
Below in conjunction with embodiment, being clearly and completely described the technical scheme in invention, described embodiment is only
The part of the present invention rather than whole embodiments.
Embodiment 1:
Use following processing step can be prepared into the organic multiple complex feed additive with blackstrap as primary raw material:
(1) take blackstrap that weight portion is 10 parts, bean dregs that Semen Maydis powder that weight portion is 5 parts, weight portion are 5 parts, weight portion are 5
Part Testa oryzae, weight portion be the fermented bacterium of 0.1 part and trace element that weight portion is 0.1 part, standby；Described trace element
Composition and each component parts by weight: 1 part of ferrous sulfate, 1 part of zinc sulfate, 0.5 part of copper sulfate, calcium oxalate 2 parts, manganese sulfate
1 part；The weight water content of described blackstrap is 15%-25%.
(2) heat in blackstrap being placed in reactor by above-mentioned parts by weight, keep temperature in the kettle between 85-105 DEG C,
Adding a kind of described trace element respectively to react 1-2 hour, course of reaction is stirred continuously, and obtains reaction solution every time；
(3) adding alkali in reaction solution, regulation pH is between 6.0-7.0；Described alkali is sodium hydroxide, sodium carbonate, hydroxide
One in potassium, ammonium hydroxide or potassium carbonate.
(4) after the reaction solution natural cooling adding alkali, Semen Maydis powder, Testa oryzae and fermented bacterium mix homogeneously are added laggard
Row anaerobic fermentation is cultivated 7-15 days；Obtain premix material, seal standby；
(5) carry out anaerobic fermentation with premix material after being mixed homogeneously by bean dregs again to cultivate 7-15 days；I.e. obtain with blackstrap as raw material
Organic multiple complex feed additive.
The preparation process of fermented bacterium is:
(1) first cell culture medium of strain is cultivated: will be commercially available strain and cultivate 1-2 days at 22-35 DEG C with first cell culture medium respectively；
Described strain includes that candidiasis, bacillus subtilis, lactic acid bacteria, photosynthetic bacteria, whiterot fungi, Trichoderma viride, marsh are red
Pseudomonas, streptococcus faecalis and cellulase；
The raw material weight mark of described first cell culture medium is: glucose 5%, agar powder 5%, potassium dihydrogen phosphate 3%, sodium chloride 2%,
Ferrous sulfate 1%, zinc sulfate 1%, herbage juice 5%, remaining is the mashed potato boiled；Preparation process is the tune that added water by above-mentioned raw materials
Pasty state is become to cook 5-10 minute；Described herbage juice is graminous pasture or leguminous forage, including timothy grass (timothy), open country
Thatch (orchard grass), bluegrass (kentucky blue grass), thin wheat, Pennisetum hydridum, Arundo donax grass, fescue grass, Rice dumpling leaf, Canis familiaris L.
Tail grass, alfalfa, Herba Trifolii Pratentis, Russian red clover, one or more in Caulis arachidis hypogaeae, vetch and Herba Kummerowiae Striatae, preparation process is
Above-mentioned fresh forage is broken into juice, does not filter directly use.
(2) secondary medium of strain is cultivated: after all strains one-level cultivated merge according to equivalent weight ratio, put
Inoculate in secondary medium, cultivate 3-5 days at 22-35 DEG C, obtain fermented bacterium；
The raw material weight number of described secondary medium is: 5 parts of brown sugar, Semen Maydis powder 5 parts, 2 parts of sodium chloride, potassium dihydrogen phosphate 3
Part, 1 part of ferrous sulfate, 1 part of zinc sulfate, 5 parts of herbage juice, 10 parts of Testa Tritici, the mashed potato boiled 10 parts；Preparation process is by upper
State the raw material furnishing pasty state that adds water and cook 5-10 minute；Secondary medium add after cooking from cattle and sheep ruminant stomach take out stomach slag and
Gastric juice mix homogeneously, parts by weight are the 1% of secondary medium gross weight.
Embodiment 2
Use following processing step can be prepared into the organic multiple complex feed additive with blackstrap as primary raw material:
(1) take blackstrap that weight portion is 19 parts, bean dregs that Semen Maydis powder that weight portion is 8 parts, weight portion are 7 parts, weight portion are 6
Part Testa oryzae, weight portion be the fermented bacterium of 0.5 part and trace element that weight portion is 0.2 part, standby；Described trace element
Composition and each component parts by weight: 3 parts of ferrous sulfate, 2 parts of zinc sulfate, 0.8 part of copper sulfate, calcium oxalate 3 parts, manganese sulfate
2 parts；The weight water content of described blackstrap is 15%-25%.
(2) heat in blackstrap being placed in reactor by above-mentioned parts by weight, keep temperature in the kettle between 85-105 DEG C,
Adding a kind of described trace element respectively to react 1-2 hour, course of reaction is stirred continuously, and obtains reaction solution every time；
(3) adding alkali in reaction solution, regulation pH is between 6.0-7.0；Described alkali is sodium hydroxide, sodium carbonate, hydroxide
One in potassium, ammonium hydroxide or potassium carbonate.
(4) after the reaction solution natural cooling adding alkali, Semen Maydis powder, Testa oryzae and fermented bacterium mix homogeneously are added laggard
Row anaerobic fermentation is cultivated 7-15 days；Obtain premix material, seal standby；
(5) carry out anaerobic fermentation with premix material after being mixed homogeneously by bean dregs again to cultivate 7-15 days；I.e. obtain with blackstrap as raw material
Organic multiple complex feed additive.
The preparation process of fermented bacterium is:
(1) first cell culture medium of strain is cultivated: will be commercially available strain and cultivate 1-2 days at 22-35 DEG C with first cell culture medium respectively；
Described strain includes that candidiasis, bacillus subtilis, lactic acid bacteria, photosynthetic bacteria, whiterot fungi, Trichoderma viride, marsh are red
Pseudomonas, streptococcus faecalis and cellulase；
The raw material weight mark of described first cell culture medium is: glucose 6%, agar powder 7%, potassium dihydrogen phosphate 4%, sodium chloride 3%,
Ferrous sulfate 2%, zinc sulfate 1.5%, herbage juice 8%, remaining is the mashed potato boiled；Preparation process is to be added water by above-mentioned raw materials
Furnishing pasty state cooks 5-10 minute；Described herbage juice is graminous pasture or leguminous forage, including timothy grass (timothy),
Orchard grass (orchard grass), bluegrass (kentucky blue grass), thin wheat, Pennisetum hydridum, Arundo donax grass, fescue grass, Rice dumpling leaf,
Herba Setariae Viridis, alfalfa, Herba Trifolii Pratentis, Russian red clover, one or more in Caulis arachidis hypogaeae, vetch and Herba Kummerowiae Striatae, preparation process
It is that above-mentioned fresh forage is broken into juice, does not filter directly use.
(2) secondary medium of strain is cultivated: after all strains one-level cultivated merge according to equivalent weight ratio, put
Inoculate in secondary medium, cultivate 3-5 days at 22-35 DEG C, obtain fermented bacterium；
The raw material weight number of described secondary medium is: 6 parts of brown sugar, Semen Maydis powder 6 parts, 4 parts of sodium chloride, potassium dihydrogen phosphate 4
Part, 2 parts of ferrous sulfate, 1.5 parts of zinc sulfate, 7 parts of herbage juice, 14 parts of Testa Tritici, the mashed potato boiled 15 parts；Preparation process be by
The above-mentioned raw materials furnishing pasty state that adds water cooks 5-10 minute；Secondary medium adds the stomach slag taken out from cattle and sheep ruminant stomach after cooking
And gastric juice mix homogeneously, parts by weight are the 2% of secondary medium gross weight.
Embodiment 3
Use following processing step can be prepared into the organic multiple complex feed additive with blackstrap as primary raw material:
(1) take blackstrap that weight portion is 25 parts, bean dregs that Semen Maydis powder that weight portion is 10 parts, weight portion are 8 parts, weight portion are
The Testa oryzae of 8 parts, weight portion are the fermented bacterium of 1 part and trace element that weight portion is 0.3 part, standby；Described trace element
Composition and each component parts by weight: 5 parts of ferrous sulfate, 4 parts of zinc sulfate, calcium oxalate 5 parts, manganese sulfate 4 parts, copper sulfate 1
Part；The weight water content of described blackstrap is 15%-25%.
(2) heat in blackstrap being placed in reactor by above-mentioned parts by weight, keep temperature in the kettle between 85-105 DEG C,
Adding a kind of described trace element respectively to react 1-2 hour, course of reaction is stirred continuously, and obtains reaction solution every time.
(3) adding alkali in reaction solution, regulation pH is between 6.0-7.0；Described alkali is sodium hydroxide, sodium carbonate, hydrogen
One in potassium oxide, ammonium hydroxide or potassium carbonate.
(4) after the reaction solution natural cooling adding alkali, Semen Maydis powder, Testa oryzae and fermented bacterium mix homogeneously are added laggard
Row anaerobic fermentation is cultivated 7-15 days；Obtain premix material, seal standby.
(5) carry out anaerobic fermentation with premix material after being mixed homogeneously by bean dregs again to cultivate 7-15 days；I.e. obtain with blackstrap be
The organic multiple complex feed additive of raw material.
The preparation process of fermented bacterium is:
(1) first cell culture medium of strain is cultivated: will be commercially available strain and cultivate 1-2 days at 22-35 DEG C with first cell culture medium respectively；
Described strain includes that candidiasis, bacillus subtilis, lactic acid bacteria, photosynthetic bacteria, whiterot fungi, Trichoderma viride, marsh are red
Pseudomonas, streptococcus faecalis and cellulase；
The raw material weight mark of described first cell culture medium is: glucose 8%, agar powder 8%, potassium dihydrogen phosphate 5%, sodium chloride 5%,
Ferrous sulfate 3%, zinc sulfate 2%, herbage juice 10%, remaining is the mashed potato boiled；Preparation process is the tune that added water by above-mentioned raw materials
Pasty state is become to cook 5-10 minute；Described herbage juice is graminous pasture or leguminous forage, including timothy grass (timothy), open country
Thatch (orchard grass), bluegrass (kentucky blue grass), thin wheat, Pennisetum hydridum, Arundo donax grass, fescue grass, Rice dumpling leaf, Canis familiaris L.
Tail grass, alfalfa, Herba Trifolii Pratentis, Russian red clover, one or more in Caulis arachidis hypogaeae, vetch and Herba Kummerowiae Striatae, preparation process is
Above-mentioned fresh forage is broken into juice, does not filter directly use.
(2) secondary medium of strain is cultivated: after all strains one-level cultivated merge according to equivalent weight ratio, put
Inoculate in secondary medium, cultivate 3-5 days at 22-35 DEG C, obtain fermented bacterium；
The raw material weight number of described secondary medium is: 8 parts of brown sugar, Semen Maydis powder 8 parts, 5 parts of sodium chloride, potassium dihydrogen phosphate 5
Part, 3 parts of ferrous sulfate, 2 parts of zinc sulfate, 10 parts of herbage juice, 15 parts of Testa Tritici, the mashed potato boiled 20 parts；Preparation process be by
The above-mentioned raw materials furnishing pasty state that adds water cooks 5-10 minute；Secondary medium adds the stomach slag taken out from cattle and sheep ruminant stomach after cooking
And gastric juice mix homogeneously, parts by weight are the 3% of secondary medium gross weight.
Application Example
100 Xia Nanniu are divided into two groups to carry out feeding experiment, matched group and experimental group by 1, Hengxian County's animal husbandry company limited
Each 50.Control group fed grass silage 50 parts, draft beer slag 10 parts, concentrate 30 parts and 5 parts of molasses, feed three every day, former
First weight average is 155.85 kilograms, and after feeding 100 days, weight average is 246.25 kilograms, and in feeding process, cattle easily occurs
Get angry, poor appetite.Experimental group feeds organic multiple with blackstrap as primary raw material utilizing that the method for the present invention prepares
Complex feed additive adds in grass silage material, and herbage per ton adds 3-5 kilogram, feeds every day three times, original body weight
Average out to 155.45 kilograms, after feeding 100 days, weight average is 287.54 kilograms, without chemical drugs in feeding process, raises
Since feeding more than a year, cattle did not occurred getting angry and other gastroenteropathy, cattle good appetite.
Described above is not limitation of the present invention, and the present invention is also not limited to examples detailed above, the art general
Logical technical staff, in the essential scope of the present invention, the change made, retrofits, adds or replaces, should belong to the guarantor of the present invention
Protect scope.
Claims (5)
1. the method being the organic multiple complex feed additive of raw material production with blackstrap, it is characterised in that: its blackstrap and
Trace element is after multicomponent complexation reaction, then obtains through ferment in second time with Semen Maydis powder, bean dregs, Testa oryzae and fermented bacterium；
The weight portion of described blackstrap is 10-25 part；Semen Maydis powder 5-10 part；Bean dregs 5-8 part；Testa oryzae 5-8 part；Fermented bacterium
0.1-1 part；Trace element 0.1-0.3 part；
The composition of described trace element and each component parts by weight: ferrous sulfate 1-5 part, zinc sulfate 1-4 part, calcium oxalate
2-5 part, manganese sulfate 1-4 part, copper sulfate 0.5-1 part；
The weight water content of described blackstrap is 15%-25%.
The most according to claim 1 be the organic multiple complex feed additive of raw material production with blackstrap method, its
It is characterised by: the preparation process of described fermented bacterium is:
(1) first cell culture medium of strain is cultivated: will be commercially available strain and cultivate 1-2 days at 22-35 DEG C with first cell culture medium respectively；
Described strain includes that candidiasis, bacillus subtilis, lactic acid bacteria, photosynthetic bacteria, whiterot fungi, Trichoderma viride, marsh are red
Pseudomonas, streptococcus faecalis and cellulase；
The raw material weight mark of described first cell culture medium is: glucose 5-8%, agar powder 5-8%, potassium dihydrogen phosphate 3-5%, chlorine
Changing sodium 2-5%, ferrous sulfate 1-3%, zinc sulfate 1-2%, herbage juice 5-10%, remaining is the mashed potato boiled；Preparation process is
The furnishing pasty state that added water by above-mentioned raw materials cooks 5-10 minute；
(2) secondary medium of strain is cultivated: after all strains one-level cultivated merge according to equivalent weight ratio, be put into two
Level inoculation of medium, cultivates 3-5 days at 22-35 DEG C, obtains fermented bacterium；
The raw material weight number of described secondary medium is: brown sugar 5-8 part, Semen Maydis powder 5-8 part, sodium chloride 2-5 part, di(2-ethylhexyl)phosphate
Hydrogen potassium 3-5 part, ferrous sulfate 1-3 part, zinc sulfate 1-2 part, herbage juice 5-10 part, Testa Tritici 10-15 part, the mashed potato boiled
10-20 part；Preparation process is that the furnishing pasty state that added water by above-mentioned raw materials cooks 5-10 minute；Secondary medium adds from cattle after cooking
The stomach slag taken out in sheep ruminant stomach and gastric juice mix homogeneously, parts by weight are the 1-3% of secondary medium gross weight.
The most according to claim 1 be the organic multiple complex feed additive of raw material production with blackstrap method, its
It is characterised by: the steps include:
(1) heat in blackstrap being placed in reactor by above-mentioned parts by weight, keep temperature in the kettle between 85-105 DEG C, every time
The component adding a kind of trace element is respectively reacted 1-2 hour, and course of reaction is stirred continuously, and obtains reaction solution；
(2) adding alkali in reaction solution, regulation pH is between 6.0-7.0；Described alkali is sodium hydroxide, sodium carbonate, hydroxide
One in potassium, ammonium hydroxide or potassium carbonate；
(3) after the reaction solution natural cooling adding alkali, detest after adding Semen Maydis powder, Testa oryzae and fermented bacterium mix homogeneously
Aerobe fermentation is cultivated 7-15 days；Obtain premix material, seal standby；
(4) carry out anaerobic fermentation with premix material after being mixed homogeneously by bean dregs again to cultivate 7-15 days；I.e. obtain with blackstrap as raw material
Organic multiple complex feed additive.
The most according to claim 1 be the organic multiple complex feed additive of raw material production with blackstrap method, its
It is characterised by: described herbage juice is graminous pasture or leguminous forage, including timothy grass (timothy), orchard grass (orchard
Grass), bluegrass (kentucky blue grass), thin wheat, Pennisetum hydridum, Arundo donax grass, fescue grass, Rice dumpling leaf, Herba Setariae Viridis, purple lucerne
Mu, Herba Trifolii Pratentis, Russian red clover, one or more in Caulis arachidis hypogaeae, vetch and Herba Kummerowiae Striatae, preparation process is by above-mentioned fresh
Forage breaks into juice, does not filter directly use.
5. described in claim 1 with blackstrap for the method for the organic multiple complex feed additive of raw material production obtained by
Feedstuff additive product.
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| CN110833116A (en) * | 2019-10-24 | 2020-02-25 | 广西泓尚科技有限责任公司 | Preparation method of yeast concentrated powder composite ore |
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