CN105685486A - Preparation method of natural high-fiber odorless cattle and sheep feed - Google Patents
Preparation method of natural high-fiber odorless cattle and sheep feed Download PDFInfo
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Abstract
The invention relates to a preparation method of a natural high-fiber odorless cattle and sheep feed. The prepared mixed probiotic dry powder and chopped straw feed are mixed and fermented for more than 10 days to obtain the product; the straw feed is bagasse or sugarcane leaves, cornstalks or corncobs, green pasture and mulberry stems; and the fermentation probiotics comprises saccharomyces cerevisiae, EM (Effective microorganisms) and bacillus subtilis. The preparation method comprises the steps of: mixing and crushing the following components of the feed in parts by weight: 20-30 parts of soybean meal, 10-20 parts of peanut bran, 5-10 parts of passion fruit leaf, 15-20 parts of mulberry stem, 1-3 parts of momordica grosvenori and 10-20 parts of corn; mixing with 10-25 parts by weight of running water and stirring for 15min; pouring the prepared mixed probiotic raw powder and trichoderma viride in a ratio of 1kg/t; barreling and sealing; fermenting naturally for 3-7 days; and when the fermented product emits sweet fragrance, pouring the fermented product into a stirrer, and adding 20-40 parts by weight of rice bran and stirring uniformly to obtain the fermented probiotic dry powder.
Description
Technical field
The invention belongs to animal feeding technology, the preparation method being specifically related to a kind of natural high microsteping odorless type cattle and sheep feedstuff。
Background technology
Carnis caprae seu ovis, beef is indispensable in everyday home living and diet, raising along with people's living standard, the requirement of quality of the life is also increased day by day, meat sheep on the market at present, beef cattle is in the process raised, for disease preventing and treating, reduce mortality rate, generally all can add antibiotic in feedstuff, and the edible antibiotic of Long term Animal can develop immunity to drugs, even at internal generation toxin, these toxic and side effects all can along with Carnis caprae seu ovis, beef is taken in by human body, health is caused great threat, therefore major part consumer is more likely to the beef and mutton of green ecological cultivation, but the cultural method feed cost of pure natural is high, in cattle and sheep feedstuff slower containing a large amount of cellulosic fermenting raw materials, and nutrition is unbalanced, cause delivering body weight for sale partially light。Currently also there is manufacturer in research and development without adding antibiotic cattle and sheep feedstuff, the following is the Patents retrieved:
1, Chinese patent: a kind of cattle and sheep feedstuff;Application number: 201510447415.8;The applying date: 2015-07-24;Applicant: Ren Hong;Address: 235000 Suixi, Huaibei City Suixi County, Anhui Province Zhen Dai He Lu109 institutes 27;Summary: the present invention relates to a kind of cattle and sheep feedstuff, including the raw material of following percentage by weight: agricultural crop straw 50%~60%, wheat bran 20%~30%, Semen Maydis 10%~15%, grain material 3%~5%, stone powder 0.2%~0.5%, calcium hydrogen phosphate 1%~2%, Sal 1%~2%, premix material 2%~3%。Make full use of agricultural crop straw, and provide enough nutritional needs for the growth of cattle and sheep。
2, Chinese patent: a kind of cattle and sheep feedstuff;Application number: 201310658202.0;The applying date: 2013-12-09;Applicant: Wang Chunyan;Address: Room 211,110164 Hui Shan street, Shenbeixin District, Shenyang City, Liaoning Province 123-7;Summary: a kind of cattle and sheep feedstuff, belongs to field of feed, and this feedstuff includes by weight percentage: Semen Glycines powder 30%, Semen Maydis powder 10%, Radix Glycyrrhizae 30%, fresh grass fiber powder 20%, aminoacid 5%, trace element 5%。Advantages of the present invention: nutritious, environmental protection。
3, Chinese patent: a kind of cattle and sheep feedstuff;Application number: 201310448975.6;The applying date: 2013-09-28;Applicant: Anhui Jin Mu feed corporation,Ltd;Address: 236000 Zhenxing Roads, Fuyang City grain husk East, Anhui Province;Summary: the present invention relates to field of feed, it is specifically related to a kind of cattle and sheep feedstuff, this feedstuff is obtained by the proportioning raw materials of following percentage by weight: Semen Maydis 60-80 part, bean cake 20-30 part, cottonseed meal 12-15 part, DDGS4-5 part, citric acid waste 3-4 part, Herba Medicaginis straw 5-6 part, rye grass 3-4 part, Allium macrostemon 2-3 part, premix material 1-2 part, prairie milk vetch stem and leaf 4-5 part, wheat-middlings 6-8 part, calcium hydrogen phosphate 1-2 part, stone powder 1-1.5 part, premix material 1-1.5 part, proper amount of salt, phagostimulant 0.2-0.5 part, the cattle and sheep feed nutrition that the present invention prepares is comprehensive, after cattle and sheep are edible, weightening finish is substantially, the rye grass added, Allium macrostemon, the raw materials such as prairie milk vetch stem and leaf, containing various active composition, cattle and sheep daily gain can be effectively improved, also improve the palatability of feedstuff, effectively reduce feed-weight ratio, the utilization rate of feedstuff increases, aquaculture cost is reduced。
Chinese patent: Folium Agaves Sisalanae waste residue produces the method for cattle, sheep feedstuff;Application number: 201410523713.6;The applying date: 2014-10-08;Applicant: Nan Jian bio tech ltd, Guangxi;Address: 532112 universal love branches, Shan Xu farm, Shan Xu town, Fusui County, Chongzuo City, Guangxi Zhuang Autonomous Region 100;Summary: the invention discloses a kind of method that Folium Agaves Sisalanae waste residue produces cattle, sheep feedstuff, it is to be formed by the raw material production of following parts by weight: Folium Agaves Sisalanae waste residue: 100-150;Caulis Sacchari sinensis end pin: 50-100;Fresh filter mud: 10-20;Compound bacteria 0.1-0.5;Its production method is that by weight 1:1, the Folium Agaves Sisalanae waste residue that processing brand-name computer presses out is added water, and add Caulis Sacchari sinensis end pin, fresh filter mud and compound bacteria by weight, put into fermenting cellar fermentation, fermenting cellar temperature controls at 30-50 DEG C, ferment and obtain fermented feed in 4-8 days, through dried, obtain cattle, sheep feedstuff。Cattle that Folium Agaves Sisalanae waste residue of the present invention is produced, sheep feedstuff, can reduce the consumption of effective protein feeds, thus being substantially reduced raising totle drilling cost, the finished product cattle, the sheep feedstuff color and luster that obtain after fermentation are good, nutritious, abnormal smells from the patient delicate fragrance, excellent taste, is well suited for feeding cattle, sheep。
Summary of the invention
It is an object of the invention to provide a kind of natural high microsteping odorless type cattle and sheep feedstuff and preparation method thereof。
Technical scheme is as follows:
By derived above for the straw feed mixed fermentation of mixing probiotic bacteria dry powder and chopping for preparing 10 days, described straw feed is bagasse or leaf, corn stalk or rod core, blue or green herbage and Mulberry bar, the raw material of described feedstuff and parts by weight are:
Bagasse or leaf 20-40;
Blue or green herbage 20-40;
Corn stalk or rod core 10-20;
Mulberry bar 10-20;
Trichoderma viride 0.1-0.5;
Mixing probiotic bacteria dry powder 0.1-0.5;
Described fermentation probiotic bacteria dry powder includes the former powder of the mixed vaccine with saccharomyces cerevisiae, EM bacterium and bacillus cereus and adds the material that the cultivation of amplification culture base obtains, and the parts by weight of amplification culture based raw material are:
Bean cake 20-30, peanut residue 10-20, leaf of Passifolra edulis 5-10, Mulberry bar 15-20, Fructus Momordicae 1-3, Semen Maydis 10-20 and tap water 10-25;
Incubation is: by bean cake, peanut residue, leaf of Passifolra edulis, Mulberry bar, Fructus Momordicae, crush maize and mix addition tap water and stir after 15 minutes, pour the former powder of the mixed vaccine prepared and Trichoderma viride in the ratio of 1kg/t and barrelling seals, normal temperature fermentation 3-7 days, after tunning sends sweet fragrance, tunning is poured in blender, add the Testa oryzae that parts by weight are 20-40 to stir, obtain fermentation probiotic bacteria dry powder;
The former powder of described mixed vaccine is to mix domestication after the saccharomyces cerevisiae of purchase, EM bacterium and bacillus cereus being cultivated respectively respectively to obtain;
It is that saccharomyces cerevisiae is placed in culture medium culturing that described saccharomyces cerevisiae is cultivated, and culture medium is added 70% sterile distilled water after mixing formed by the peptone of weight content 3%, 5% glucose, 20% rice meal, 2% potassium dihydrogen phosphate;Cultural method is to be heated to 100 DEG C by adding sterile distilled water after peptone, glucose, potassium dihydrogen phosphate mixing, waits addition saccharomyces cerevisiae Mixed culture after being cooled to room temperature to save backup for more than 2 days;
It is that EM bacterium is placed in culture medium culturing that described EM bacterium is cultivated, EM bacterium culture medium adds 75% sterile distilled water after being mixed by weight content 2% brown sugar, 20% Semen Maydis powder and 3% beerwort and forms, cultural method is after first being sterilized by culture dish high temperature sterilization, with hot water, brown sugar is melted, add sterile distilled water, heat the water to 100 DEG C, add Semen Maydis powder, beerwort mixing, thing to be mixed in the pasty state, adds the cultivation of EM bacterium and saves backup for more than 2 days after being cooled to room temperature;
It is that bacillus subtilis is placed in culture medium culturing that described bacillus subtilis is cultivated, bacillus subtilis bacterium culture medium is by weight content 5% Carnis Bovis seu Bubali cream, 5% glucose, 10% peptone starch, 2% sodium chloride and 3% calcium carbonate, add the sterile distilled water composition of 80%, cultural method is that sterile distilled water is heated to 100 DEG C, Carnis Bovis seu Bubali cream, glucose, peptone starch, sodium chloride and calcium carbonate being stirred, thing to be mixed adds bacillus subtilis cultivation and saves backup for more than 2 days after being cooled to room temperature in the pasty state again;
By above-mentioned strain three kinds standby according to strain weight ratio it is: saccharomyces cerevisiae: EM bacterium: bacillus cereus mixing=1:1:1, add mixing probiotic bacteria culture medium to cultivate, described mixing probiotic bacteria culture medium is made up of 10% Carnis Bovis seu Bubali cream, 5% glucose, 5% beerwort, 5% Semen Maydis powder, 3% brown sugar, 3% calcium carbonate, 4% sodium chloride, after adding sterile distilled water 65% mixing, being heated to 100 DEG C, thing to be mixed adds 2 days former powder of mixed vaccine derived above of spawn culture three kinds standby after being cooled to room temperature in the pasty state。
Above-described saccharomyces cerevisiae (Saccharomycescerevisiae) saccharomyces cerevisiae is and human relation's primary yeast the most widely, except can making wine, it is also possible to be used for making bread andSteamed breadDeng food, the saccharomyces cerevisiae that the present invention specifically adopts is rice wine yeast, and its thalline vitamin, protein content are high, can eat, medicinal and feed yeast, it is also possible to extract from whichCytochrome C, nucleic acid, glutathion, thromboplastinum, coenzyme A andAdenosine triphosphateDeng。
Above-described EM bacterium, EM bacterium is the english abbreviation of effective microorganisms (EffectiveMicroorganisms), also being EM probiotic bacteria stock solution, it can make 10 in the big quasi-microorganism of filamentous bacteria five of photosynthetic bacteria, lactic acid bacteria, yeast, Gram positive actinomycetes, fermentation system belong to 80 kinds of beneficial microbe coexistence and co-prosperities。It is shone husband's teaching inventive by university of Ryukyu of world-renowned using microbe scholar Japan than good, feedstuff is inoculated EM bacterium, natural fermentation speed can be improved, improve efficiency, probiotics contained by EM bacterium itself is provided that the single cell protein needed for animal, significantly improve the daily gain of animal, promote economic benefit;And EM bacterium can improve animal body environment, improve animal immunizing power, reduce pathogenicity rate and mortality rate。
Above-described bacillus cereus (Bacillus), bacillus cereus can produce multiple digestive enzyme, helps animal digesting and assimilating nutrient substance。Bacillus cereus has stronger protease, amylase and lipase active, also there is the enzyme of complex carbohydrates in degraded feedstuff simultaneously, such as pectase, glucanase, cellulase etc., these enzymes can destroy the cell wall of plant feed cell, the nutrient substance promoting cell discharges, and the antinutritional factor in feedstuff can be eliminated, reduce the obstacle that animal digestion is utilized by antinutritional factor, bacillus cereus enters in animal body, regulating intestinal canal flora can be played, maintain the effect of microecological balance, thus improving the gastrointestinal function of animal。
Above-described Trichoderma viride (Trichodermaviride) Trichoderma viride is one of bacterial strain that institute's cellulase-producing activity is the highest; crop is had Degradation by produced cellulase; effect is very good; cellulose decomposition speed can be greatly improved; improve product albumen matter content; Trichoderma viride is again a kind of resourceful antagonistic microbe, has protection and treatment double effects。
The invention has the beneficial effects as follows:
1, the present invention adds, in the process producing feedstuff, the mixing probiotic bacteria provided for oneself, after making the feedstuff that the edible present invention of beef cattle, meat sheep produces, improves animal body environment, removes animal wastes foul smell, be conducive to the construction of plant's odorless environment。
2, the present invention adds, in the process producing feedstuff, the mixing probiotic bacteria provided for oneself, mixing probiotics fermention product comprises probiotic bacteria and can kill pathogen and harmful levels of pathogens, improve beef cattle, the immunity of meat sheep own, antibiotic need not be added in feedstuff, after making the feedstuff that the edible present invention of beef cattle, meat sheep produces, hence it is evident that reduce morbidity rate and mortality rate。
3, the present invention adds, in the process producing feedstuff, the mixing probiotic bacteria provided for oneself, and after making the feedstuff that the edible present invention of beef cattle, meat sheep produces, daily gain significantly improves, within 15-20 days, deliver for sale in advance, and after the feedstuff that produces of beef cattle, the edible present invention of meat sheep, personality is docile, not fighting, disorderly string does not dap。
4, the feedstuff that the present invention produces utilizes pure natural fermented material, less costly, and without antibiotic and hormone, it is ensured that feed nutrition is balanced, reduces green cultivation cost。
5, with the addition of leaf of Passifolra edulis and Ramulus Mori in the feedstuff that the present invention produces, Ramulus Mori is containing abundant crude protein, cellulose, lignin and half fiber;Leaf of Passifolra edulis has effect of resolving heat and reducing pathogenic fire, contributes to adjusting beef cattle, meat sheep Intestinum Sus domestica stomach, reduces feces foul smell。
6, with the addition of Fructus Momordicae in the feedstuff that the present invention produces, Fructus Momordicae itself is containing another containing nutritional labelings such as fructose, aminoacid, flavone, and beef cattle, the edible rear meat of meat sheep have fresh and sweet mouthfeel。
7, the present invention with the addition of Trichoderma viride co-fermentation, accelerates a large amount of leaf of Passifolra edulis and the speed of Mulberry bar decomposition, improves dietary protein level, increase feed nutrient。
Detailed description of the invention
Embodiment 1
First the former powder of mixed vaccine is prepared, mix domestication after the saccharomyces cerevisiae of purchase, EM bacterium and bacillus cereus being cultivated respectively respectively to obtain, it is that saccharomyces cerevisiae is placed in culture medium culturing that described saccharomyces cerevisiae is cultivated, and culture medium is added 70% sterile distilled water after mixing formed by the peptone of weight content 3%, 5% glucose, 20% rice meal, 2% potassium dihydrogen phosphate;Cultural method is to be heated to 100 DEG C by adding sterile distilled water after peptone, glucose, potassium dihydrogen phosphate mixing, waits addition saccharomyces cerevisiae Mixed culture after being cooled to room temperature to save backup for more than 2 days;
It is that EM bacterium is placed in culture medium culturing that described EM bacterium is cultivated, EM bacterium culture medium adds 75% sterile distilled water after being mixed by weight content 2% brown sugar, 20% Semen Maydis powder and 3% beerwort and forms, cultural method is after first being sterilized by culture dish high temperature sterilization, with hot water, brown sugar is melted, add sterile distilled water, heat the water to 100 DEG C, add Semen Maydis powder, beerwort mixing, thing to be mixed in the pasty state, adds the cultivation of EM bacterium and saves backup for more than 2 days after being cooled to room temperature;
It is that bacillus subtilis is placed in culture medium culturing that described bacillus subtilis is cultivated, bacillus subtilis bacterium culture medium is by weight content 5% Carnis Bovis seu Bubali cream, 5% glucose, 10% peptone starch, 2% sodium chloride and 3% calcium carbonate, add the sterile distilled water composition of 80%, cultural method is that sterile distilled water is heated to 100 DEG C, Carnis Bovis seu Bubali cream, glucose, peptone starch, sodium chloride and calcium carbonate being stirred, thing to be mixed adds bacillus subtilis cultivation and saves backup for more than 2 days after being cooled to room temperature in the pasty state again;
By above-mentioned strain three kinds standby according to strain weight ratio it is: saccharomyces cerevisiae: EM bacterium: bacillus cereus mixing=1:1:1, add mixing probiotic bacteria culture medium to cultivate, described mixing probiotic bacteria culture medium is made up of 10% Carnis Bovis seu Bubali cream, 5% glucose, 5% beerwort, 5% Semen Maydis powder, 3% brown sugar, 3% calcium carbonate, 4% sodium chloride, after adding sterile distilled water 65% mixing, being heated to 100 DEG C, thing to be mixed adds 2 days former powder of mixed vaccine derived above of spawn culture three kinds standby after being cooled to room temperature in the pasty state。
Then fermentation probiotic bacteria dry powder is prepared, former for the mixed vaccine of saccharomyces cerevisiae, EM bacterium and bacillus cereus powder being added amplification culture base and cultivates the material obtained, the parts by weight of amplification culture based raw material are: bean cake 20, peanut residue 10, leaf of Passifolra edulis 5, Mulberry bar 15, Fructus Momordicae 1, Semen Maydis 10 and tap water 10;Incubation is: by bean cake, peanut residue, leaf of Passifolra edulis, Mulberry bar, Fructus Momordicae, crush maize and mix addition tap water and stir after 15 minutes, the former powder of the mixed vaccine prepared is poured in the ratio of 1kg/t, and barrelling seals, normal temperature fermentation 5 days, after tunning sends sweet fragrance, tunning is poured in blender, add the Testa oryzae that parts by weight are 20 and stir, obtain fermentation probiotic bacteria dry powder;
Finally straw feed is respectively formed and mix according to following weight ratio: bagasse or leaf 20, blue or green herbage 20, corn stalk or rod core 10, Mulberry bar 10, Trichoderma viride 0.1 and fermentation probiotic bacteria dry powder 0.1 mixed fermentation obtain tunning in 15 days, packed products, gained feedstuff seals to preserve and never degenerates half a year more than。
Embodiment 2
First the former powder of mixed vaccine is prepared, mix domestication after the saccharomyces cerevisiae of purchase, EM bacterium and bacillus cereus being cultivated respectively respectively to obtain, it is that saccharomyces cerevisiae is placed in culture medium culturing that described saccharomyces cerevisiae is cultivated, and culture medium is added 70% sterile distilled water after mixing formed by the peptone of weight content 3%, 5% glucose, 20% rice meal, 2% potassium dihydrogen phosphate;Cultural method is to be heated to 100 DEG C by adding sterile distilled water after peptone, glucose, potassium dihydrogen phosphate mixing, waits addition saccharomyces cerevisiae Mixed culture after being cooled to room temperature to save backup for more than 2 days;
It is that EM bacterium is placed in culture medium culturing that described EM bacterium is cultivated, EM bacterium culture medium adds 75% sterile distilled water after being mixed by weight content 2% brown sugar, 20% Semen Maydis powder and 3% beerwort and forms, cultural method is after first being sterilized by culture dish high temperature sterilization, with hot water, brown sugar is melted, add sterile distilled water, heat the water to 100 DEG C, add Semen Maydis powder, beerwort mixing, thing to be mixed in the pasty state, adds the cultivation of EM bacterium and saves backup for more than 2 days after being cooled to room temperature;
It is that bacillus subtilis is placed in culture medium culturing that described bacillus subtilis is cultivated, bacillus subtilis bacterium culture medium is by weight content 5% Carnis Bovis seu Bubali cream, 5% glucose, 10% peptone starch, 2% sodium chloride and 3% calcium carbonate, add the sterile distilled water composition of 80%, cultural method is that sterile distilled water is heated to 100 DEG C, Carnis Bovis seu Bubali cream, glucose, peptone starch, sodium chloride and calcium carbonate being stirred, thing to be mixed adds bacillus subtilis cultivation and saves backup for more than 2 days after being cooled to room temperature in the pasty state again;
By above-mentioned strain three kinds standby according to strain weight ratio it is: saccharomyces cerevisiae: EM bacterium: bacillus cereus mixing=1:1:1, add mixing probiotic bacteria culture medium to cultivate, described mixing probiotic bacteria culture medium is made up of 10% Carnis Bovis seu Bubali cream, 5% glucose, 5% beerwort, 5% Semen Maydis powder, 3% brown sugar, 3% calcium carbonate, 4% sodium chloride, after adding sterile distilled water 65% mixing, being heated to 100 DEG C, thing to be mixed adds 2 days former powder of mixed vaccine derived above of spawn culture three kinds standby after being cooled to room temperature in the pasty state。
Then fermentation probiotic bacteria dry powder is prepared, former for the mixed vaccine of saccharomyces cerevisiae, EM bacterium and bacillus cereus powder being added amplification culture base and cultivates the material obtained, the parts by weight of amplification culture based raw material are: bean cake 30, peanut residue 20, leaf of Passifolra edulis 10, Mulberry bar 20, Fructus Momordicae 3, Semen Maydis 20 and tap water 25;Incubation is: by bean cake, peanut residue, leaf of Passifolra edulis, Mulberry bar, Fructus Momordicae, crush maize and mix addition tap water and stir after 15 minutes, the former powder of the mixed vaccine prepared is poured in the ratio of 1kg/t, and barrelling seals, normal temperature fermentation 5 days, after tunning sends sweet fragrance, tunning is poured in blender, add the Testa oryzae that parts by weight are 40 and stir, obtain fermentation probiotic bacteria dry powder;
Finally straw feed is respectively formed and mix according to following weight ratio: bagasse or leaf 40, blue or green herbage 40, corn stalk or rod core 20, Mulberry bar 20, Trichoderma viride 0.5 and fermentation probiotic bacteria dry powder 0.5 mixed fermentation obtain tunning in 15 days, packed products, gained feedstuff seals to preserve and never degenerates half a year more than。
Embodiment 3
First the former powder of mixed vaccine is prepared, mix domestication after the saccharomyces cerevisiae of purchase, EM bacterium and bacillus cereus being cultivated respectively respectively to obtain, it is that saccharomyces cerevisiae is placed in culture medium culturing that described saccharomyces cerevisiae is cultivated, and culture medium is added 70% sterile distilled water after mixing formed by the peptone of weight content 3%, 5% glucose, 20% rice meal, 2% potassium dihydrogen phosphate;Cultural method is to be heated to 100 DEG C by adding sterile distilled water after peptone, glucose, potassium dihydrogen phosphate mixing, waits addition saccharomyces cerevisiae Mixed culture after being cooled to room temperature to save backup for more than 2 days;
It is that EM bacterium is placed in culture medium culturing that described EM bacterium is cultivated, EM bacterium culture medium adds 75% sterile distilled water after being mixed by weight content 2% brown sugar, 20% Semen Maydis powder and 3% beerwort and forms, cultural method is after first being sterilized by culture dish high temperature sterilization, with hot water, brown sugar is melted, add sterile distilled water, heat the water to 100 DEG C, add Semen Maydis powder, beerwort mixing, thing to be mixed in the pasty state, adds the cultivation of EM bacterium and saves backup for more than 2 days after being cooled to room temperature;
It is that bacillus subtilis is placed in culture medium culturing that described bacillus subtilis is cultivated, bacillus subtilis bacterium culture medium is by weight content 5% Carnis Bovis seu Bubali cream, 5% glucose, 10% peptone starch, 2% sodium chloride and 3% calcium carbonate, add the sterile distilled water composition of 80%, cultural method is that sterile distilled water is heated to 100 DEG C, Carnis Bovis seu Bubali cream, glucose, peptone starch, sodium chloride and calcium carbonate being stirred, thing to be mixed adds bacillus subtilis cultivation and saves backup for more than 2 days after being cooled to room temperature in the pasty state again;
By above-mentioned strain three kinds standby according to strain weight ratio it is: saccharomyces cerevisiae: EM bacterium: bacillus cereus mixing=1:1:1, add mixing probiotic bacteria culture medium to cultivate, described mixing probiotic bacteria culture medium is made up of 10% Carnis Bovis seu Bubali cream, 5% glucose, 5% beerwort, 5% Semen Maydis powder, 3% brown sugar, 3% calcium carbonate, 4% sodium chloride, after adding sterile distilled water 65% mixing, being heated to 100 DEG C, thing to be mixed adds 2 days former powder of mixed vaccine derived above of spawn culture three kinds standby after being cooled to room temperature in the pasty state。
Then fermentation probiotic bacteria dry powder is prepared, former for the mixed vaccine of saccharomyces cerevisiae, EM bacterium and bacillus cereus powder being added amplification culture base and cultivates the material obtained, the parts by weight of amplification culture based raw material are: bean cake 25, peanut residue 15, leaf of Passifolra edulis 8, Mulberry bar 15, Fructus Momordicae 2, Semen Maydis 15 and tap water 20;Incubation is: by bean cake, peanut residue, leaf of Passifolra edulis, Mulberry bar, Fructus Momordicae, crush maize and mix addition tap water and stir after 15 minutes, the former powder of the mixed vaccine prepared is poured in the ratio of 1kg/t, and barrelling seals, normal temperature fermentation 5 days, after tunning sends sweet fragrance, tunning is poured in blender, add the Testa oryzae that parts by weight are 35 and stir, obtain fermentation probiotic bacteria dry powder;
Finally straw feed is respectively formed and mix according to following weight ratio: bagasse or leaf 35, blue or green herbage 35, corn stalk or rod core 15, Mulberry bar 15, Trichoderma viride 0.4 and fermentation probiotic bacteria dry powder 0.3 mixed fermentation obtain tunning in 15 days, packed products, gained feedstuff seals to preserve and never degenerates half a year more than。
Embodiment 4
First the former powder of mixed vaccine is prepared, mix domestication after the saccharomyces cerevisiae of purchase, EM bacterium and bacillus cereus being cultivated respectively respectively to obtain, it is that saccharomyces cerevisiae is placed in culture medium culturing that described saccharomyces cerevisiae is cultivated, and culture medium is added 70% sterile distilled water after mixing formed by the peptone of weight content 3%, 5% glucose, 20% rice meal, 2% potassium dihydrogen phosphate;Cultural method is to be heated to 100 DEG C by adding sterile distilled water after peptone, glucose, potassium dihydrogen phosphate mixing, waits addition saccharomyces cerevisiae Mixed culture after being cooled to room temperature to save backup for more than 2 days;
It is that EM bacterium is placed in culture medium culturing that described EM bacterium is cultivated, EM bacterium culture medium adds 75% sterile distilled water after being mixed by weight content 2% brown sugar, 20% Semen Maydis powder and 3% beerwort and forms, cultural method is after first being sterilized by culture dish high temperature sterilization, with hot water, brown sugar is melted, add sterile distilled water, heat the water to 100 DEG C, add Semen Maydis powder, beerwort mixing, thing to be mixed in the pasty state, adds the cultivation of EM bacterium and saves backup for more than 2 days after being cooled to room temperature;
It is that bacillus subtilis is placed in culture medium culturing that described bacillus subtilis is cultivated, bacillus subtilis bacterium culture medium is by weight content 5% Carnis Bovis seu Bubali cream, 5% glucose, 10% peptone starch, 2% sodium chloride and 3% calcium carbonate, add the sterile distilled water composition of 80%, cultural method is that sterile distilled water is heated to 100 DEG C, Carnis Bovis seu Bubali cream, glucose, peptone starch, sodium chloride and calcium carbonate being stirred, thing to be mixed adds bacillus subtilis cultivation and saves backup for more than 2 days after being cooled to room temperature in the pasty state again;
By above-mentioned strain three kinds standby according to strain weight ratio it is: saccharomyces cerevisiae: EM bacterium: bacillus cereus mixing=1:1:1, add mixing probiotic bacteria culture medium to cultivate, described mixing probiotic bacteria culture medium is made up of 10% Carnis Bovis seu Bubali cream, 5% glucose, 5% beerwort, 5% Semen Maydis powder, 3% brown sugar, 3% calcium carbonate, 4% sodium chloride, after adding sterile distilled water 65% mixing, being heated to 100 DEG C, thing to be mixed adds 2 days former powder of mixed vaccine derived above of spawn culture three kinds standby after being cooled to room temperature in the pasty state。
Then fermentation probiotic bacteria dry powder is prepared, former for the mixed vaccine of saccharomyces cerevisiae, EM bacterium and bacillus cereus powder being added amplification culture base and cultivates the material obtained, the parts by weight of amplification culture based raw material are: bean cake 21, peanut residue 14, leaf of Passifolra edulis 5, Mulberry bar 13, Fructus Momordicae 3, Semen Maydis 12 and tap water 18;Incubation is: by bean cake, peanut residue, leaf of Passifolra edulis, Mulberry bar, Fructus Momordicae, crush maize and mix addition tap water and stir after 15 minutes, the former powder of the mixed vaccine prepared is poured in the ratio of 1kg/t, and barrelling seals, normal temperature fermentation 5 days, after tunning sends sweet fragrance, tunning is poured in blender, add the Testa oryzae that parts by weight are 28 and stir, obtain fermentation probiotic bacteria dry powder;
Finally straw feed is respectively formed and mix according to following weight ratio: bagasse or leaf 30, blue or green herbage 28, corn stalk or rod core 16, Mulberry bar 14, Trichoderma viride 0.2 and fermentation probiotic bacteria dry powder 0.3 mixed fermentation obtain tunning in 15 days, packed products, gained feedstuff seals to preserve and never degenerates half a year more than。
The cattle and sheep feedstuff of the embodiment of the present invention compares with normal diet cowboying: kind: mora cattle
Health status | Disease condition | 12 months weight | 18 months weight | |
Embodiment 1 | Can eat snoozed, feces odorless | Sickness rate reduces by 62.3% | 120.2 kilogram | 446.4 kilogram |
Embodiment 2 | Nice love is slept, feces odorless | Sickness rate reduces by 70% | 118.5 kilogram | 482.6 kilogram |
Embodiment 3 | Can eat snoozed, feces odorless, harder | Sickness rate reduces by 59.8% | 114 kilograms | 410.3 kilogram |
Embodiment 4 | Nice love is slept, feces odorless | Sickness rate reduces by 60.2% | 123.1 kilogram | 412.8 kilogram |
Normal diet (Nanning production) | Gluttonous active, stool odor weight | Sickness rate reduces by 35.7%; | 113 kilograms | 358.5 kilogram |
Claims (3)
1. the preparation method of a natural high microsteping odorless type cattle and sheep feedstuff, it is characterized in that: be by derived above for the straw feed mixed fermentation of mixing probiotic bacteria dry powder and chopping for preparing 10 days, described straw feed is bagasse or leaf, corn stalk or rod core, blue or green herbage and Mulberry bar, the raw material of described feedstuff and parts by weight:
Bagasse or leaf 20-40;
Blue or green herbage 20-40;
Corn stalk or rod core 10-20;
Mulberry bar 10-20;
Trichoderma viride 0.1-0.5;
Mixing probiotic bacteria dry powder 0.1-0.5;
Described fermentation probiotic bacteria dry powder includes the former powder of the mixed vaccine with saccharomyces cerevisiae, EM bacterium and bacillus cereus and adds the material that the cultivation of amplification culture base obtains。
2. the preparation method of the natural high microsteping odorless type cattle and sheep feedstuff according to claims 1, it is characterised in that: the parts by weight of described amplification culture based raw material are: bean cake 20-30, peanut residue 10-20, leaf of Passifolra edulis 5-10, Mulberry bar 15-20, Fructus Momordicae 1-3, Semen Maydis 10-20 and tap water 10-25;Incubation is: by bean cake, peanut residue, leaf of Passifolra edulis, Mulberry bar, Fructus Momordicae, crush maize and mix addition tap water and stir after 15 minutes, pour the former powder of the mixed vaccine prepared and Trichoderma viride in the ratio of 1kg/t and barrelling seals, normal temperature fermentation 3-7 days, after tunning sends sweet fragrance, tunning is poured in blender, add the Testa oryzae that parts by weight are 20-40 to stir, obtain fermentation probiotic bacteria dry powder。
3. the preparation method of the natural high microsteping odorless type cattle and sheep feedstuff according to claims 1, it is characterised in that: the former powder of described mixed vaccine is to mix domestication after the saccharomyces cerevisiae of purchase, EM bacterium and bacillus cereus being cultivated respectively respectively to obtain;It is that saccharomyces cerevisiae is placed in culture medium culturing that described saccharomyces cerevisiae is cultivated, and culture medium is added 70% sterile distilled water after mixing formed by the peptone of weight content 3%, 5% glucose, 20% rice meal, 2% potassium dihydrogen phosphate;Cultural method is to be heated to 100 DEG C by adding sterile distilled water after peptone, glucose, potassium dihydrogen phosphate mixing, waits addition saccharomyces cerevisiae Mixed culture after being cooled to room temperature to save backup for more than 2 days;
It is that EM bacterium is placed in culture medium culturing that described EM bacterium is cultivated, EM bacterium culture medium adds 75% sterile distilled water after being mixed by weight content 2% brown sugar, 20% Semen Maydis powder and 3% beerwort and forms, cultural method is after first being sterilized by culture dish high temperature sterilization, with hot water, brown sugar is melted, add sterile distilled water, heat the water to 100 DEG C, add Semen Maydis powder, beerwort mixing, thing to be mixed in the pasty state, adds the cultivation of EM bacterium and saves backup for more than 2 days after being cooled to room temperature;It is that bacillus subtilis is placed in culture medium culturing that described bacillus subtilis is cultivated, bacillus subtilis bacterium culture medium is by weight content 5% Carnis Bovis seu Bubali cream, 5% glucose, 10% peptone starch, 2% sodium chloride and 3% calcium carbonate, add the sterile distilled water composition of 80%, cultural method is that sterile distilled water is heated to 100 DEG C, Carnis Bovis seu Bubali cream, glucose, peptone starch, sodium chloride and calcium carbonate being stirred, thing to be mixed adds bacillus subtilis cultivation and saves backup for more than 2 days after being cooled to room temperature in the pasty state again;By above-mentioned strain three kinds standby according to strain weight ratio it is: saccharomyces cerevisiae: EM bacterium: bacillus cereus mixing=1:1:1, add mixing probiotic bacteria culture medium to cultivate, described mixing probiotic bacteria culture medium is made up of 10% Carnis Bovis seu Bubali cream, 5% glucose, 5% beerwort, 5% Semen Maydis powder, 3% brown sugar, 3% calcium carbonate, 4% sodium chloride, after adding sterile distilled water 65% mixing, being heated to 100 DEG C, thing to be mixed adds 2 days former powder of mixed vaccine derived above of spawn culture three kinds standby after being cooled to room temperature in the pasty state。
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