CN106171762A - A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding - Google Patents
A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding Download PDFInfo
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- CN106171762A CN106171762A CN201610569050.0A CN201610569050A CN106171762A CN 106171762 A CN106171762 A CN 106171762A CN 201610569050 A CN201610569050 A CN 201610569050A CN 106171762 A CN106171762 A CN 106171762A
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- Prior art keywords
- branch
- cuttage
- seedbed
- cutting
- folium taxi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Abstract
The method that the invention discloses the breeding of a kind of wild Ramulus et folium taxi cuspidatae branch cutting; it includes the steps such as the process of cuttage branch, the process in seedbed, the cuttage of branch, final-period management; the invention have the benefit that the present invention substantially increases the survival rate of Yew cuttage breeding; the survival rate of Yew cuttage branch of the present invention is more than 95%, and the present invention makes the shielded extremely precious Ramulus et folium taxi cuspidatae seeds of country or even the world be protected;The present invention by appropriate design take off grass shade, seedling management, both reduced the seedling cost of Taxus mairei, make again entirety survival rate get a promotion;Yew cuttage branch fracture is soaked in taking root liquid by the present invention, improves the survival rate of cuttage branch.
Description
Technical field
The present invention relates to Ramulus et folium taxi cuspidatae seedling cultivation field, a kind of wild Ramulus et folium taxi cuspidatae branch cutting is numerous
The method grown.
Background technology
Ramulus et folium taxi cuspidatae has another name called Ramulus et folium taxi cuspidatae, Zhu Shu, Chi Baisong, is a kind of extremely precious seeds, can in Forest Region in Yichun, Heilongjiang Province
See that it is distributed;Yichun Ramulus et folium taxi cuspidatae material is excellent, logical straight, the compact structure of texture, and full of elasticity, mechanics temperature is high, glossy, fragrance,
Resistance to rotten, Yi Baoxiao, reflex action the most easy to crack, without Colophonium, coloring japanning bonding property is superior, especially it is appreciated that from red
Extracting paclitaxel in bean China fir, paclitaxel is widely used as a kind of cancer therapy drug novel, efficient, wide spectrum;But Ramulus et folium taxi cuspidatae
Seeds are a kind of the shielded extremely precious seeds of country or even the world, never allow to denude, and people are for many years
Studying, exploring, finding the implantation methods for Ramulus et folium taxi cuspidatae seeds, to reach the purpose to its extensive exploitation and utilization, benefiting
In the mankind.
Taxus mairei is a kind of evergreen species of taxaceae, belongs to country-level national key protected plant, resource scarcity,
And natural propagation updating ability is relatively low.But society is relatively big to its demand, can only be carried out by large area artificial growth, south
Ramulus et folium taxi cuspidatae generally uses seed seedling-raising or cuttage seedling raising method to breed in side, but existing cuttage seedling raising method survival rate is relatively low, because of
How this improves cuttage seedling raising method survival rate is problem demanding prompt solution.
Summary of the invention
Instant invention overcomes the deficiencies in the prior art, it is provided that the method for a kind of wild Ramulus et folium taxi cuspidatae branch cutting breeding, solve
The problem that existing cutting propagation survival rate is relatively low.
For solving above-mentioned technical problem, the present invention by the following technical solutions:
A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding, it comprises the following steps:
(1) selection of cuttage branch and process: select wild Ramulus et folium taxi cuspidatae to cut without the healthy and strong branch of pest and disease damage, then will
Incision position is downward, soaks 1.5-2.5h in taking root liquid;
(2) seedbed is made;
(3) cuttage of branch;
(4) final-period management;
(5) transplanting of cuttage branch or grafting.
Further, specifically comprise the following steps that
(1) process of cuttage branch: cut selecting the 2-4 month or the 9-11 month the wild Ramulus et folium taxi cuspidatae healthy and strong branch without pest and disease damage
Under, the branch sheared removes the blade close to incision position 1/3, then that incision position is downward, soaks 1.5-2.5h in taking root liquid.
(2) process in seedbed: select one or more in perlite, Lu Shi, sandy soil to make substrate, by substrate and humic
Make seedbed, described seedbed width 1-1.4m, thick 20-30cm after soil mix homogeneously, then water and make nursery soil steady and sure;
(3) cuttage of branch: step (1) soaked branch is inserted seedbed in time, during cuttage, presses standby cutting
Spacing in the rows 4-5cm, the distance of line-spacing 8-12cm insert seedbed, and the degree of depth of insertion is the 1/3 of branch length;
(4) final-period management: pour water in time after branch cutting, and on seedbed, make thin film frame, thin film spread by thin film frame;2-
The cutting seedling in April removes thin film in mid-April, changes sunshade net;The cutting seedling of the 9-11 month is removed thin at Second Year mid-April
Film, changes sunshade net;Observe situation of taking root at any time, after treating that new root length goes out, impose fertilizer;Periodically weeding, waters;The 2-4 month
Cutting seedling can be transplanted or grafting in JIUYUE then, and the cutting seedling of the 9-11 month can be transplanted or grafting at Second Year June.
Further, in described step (4), thin film frame terrain clearance is 10-20cm.
Further, in described step (4), the sunshade rate of sunshade net is 75-85%.
Further, imposing fertilizer amount in described step (4) is that every mu of seedbed imposes 10-12kg carbamide, 5-7kg
Compound fertilizer;Described compound fertilizer is that the weight quota ratio of effective ingredient is, nitrogenous fertilizer: phosphate fertilizer: potash fertilizer=3-4:4-5:4-5.
Compared with prior art, the invention has the beneficial effects as follows:
1, the present invention uses wild Ramulus et folium taxi cuspidatae to have stronger vitality, through taking root liquid without the healthy and strong branch of pest and disease damage
Wild Ramulus et folium taxi cuspidatae after process without the survival rate of the healthy and strong branch of pest and disease damage higher than the Ramulus et folium taxi cuspidatae branch of existing artificial propagation
Survival rate, and the growing way of wild Ramulus et folium taxi cuspidatae branch is better than the Ramulus et folium taxi cuspidatae branch of existing artificial propagation;The present invention substantially increases red
The survival rate of bean China fir cottage propagation, the survival rate of Yew cuttage branch of the present invention more than 95%, the present invention make country or even
Shielded extremely precious Ramulus et folium taxi cuspidatae seeds are protected in the world.
2, the present invention by appropriate design take off grass shade, seedling management, both reduced the seedling cost of Taxus mairei, again
The survival rate making entirety gets a promotion.
3, Yew cuttage branch fracture is soaked in taking root liquid by the present invention, improves the survival rate of cuttage branch.
4, the present invention provides raw material sources, extensive exploitation widely utilize and bring benefit to the mankind for medical cancer therapy drug.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.Embodiments of the present invention include but not limited to following
Embodiment.
[embodiment 1]
A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding, it comprises the following steps:
(1) process of cuttage branch: cut selecting the 2-4 month or the 9-11 month the wild Ramulus et folium taxi cuspidatae healthy and strong branch without pest and disease damage
Under, the temperature in this time typically more than 15 DEG C, less than 25 DEG C, this temperature can guarantee that cuttage branch moisture reduce evaporation and
Root heals;The rugosity of cuttage branch is 0.3-0.8cm, a length of 10-15cm, and the branch sheared removes close to incision position 1/3
Blade, then by downward for incision position, taking root liquid soaks 1.5h.
(2) process in seedbed: select perlite, road masonry substrate, a small amount of breeding to can be selected for sandy soil and make substrate, by substrate
Make seedbed, described seedbed width 1m, thick 20cm after mixing homogeneously with fertile soil, then water and make nursery soil steady and sure;
(3) cuttage of branch: step (1) soaked branch is inserted seedbed in time, during cuttage, presses standby cutting
Spacing in the rows 4cm, the distance of line-spacing 12cm insert seedbed, and the degree of depth of insertion is the 1/3 of branch length;
(4) final-period management: pour water in time after branch cutting, and on seedbed, make thin film frame, thin film frame terrain clearance is
10cm, thin film spread by thin film frame;The spring of 2-4 month bar of transplanting removes thin film in mid-April, changes sunshade net, the screening of sunshade net
Sun rate is 75%;The autumn sowing branch of the 9-11 month removes thin film at Second Year mid-April, and the sunshade rate changing sunshade net is 75%;
Observe situation of taking root at any time, intercut and keep green with rear blade, the most withered the most yellow, illustrate that management is normal, extracted until 20 days time and check,
Whether cutting base portion otch has healing sign, has cell tissue to grow, and this is the important behaviour before taking root;Spring transplants bar at cell
Tissue increases followed by takes root, after this cell tissue of autumn sowing Seedling increases, and gas after February in the coming year to be waited until after mercury dropped
Just can rush continued growth in original cell tissue after temperature rise to make new advances root;After treating that new root length goes out, impose fertilizer, impose fertilizer
Consumption is that every mu of seedbed imposes 10kg carbamide, 5kg compound fertilizer;Described compound fertilizer is that the weight quota ratio of effective ingredient is, nitrogenous fertilizer:
Phosphate fertilizer: potash fertilizer=3:4:4;Periodically weeding, waters, and can use foliage fertilizer simultaneously, within general 20 days, can spray a foliage fertilizer;2-4
The cutting seedling of the moon can be transplanted or grafting in JIUYUE then, and the cutting seedling of the 9-11 month can be transplanted or transfer at Second Year June
Connect.
The survival rate of the cuttage branch of the present embodiment is 96%.
The cutting seedling of the present embodiment being transplanted, the Second Year height of seedling of transplanting increases as 33cm.
[embodiment 2]
A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding, it comprises the following steps:
(1) process of cuttage branch: cut selecting the 2-4 month or the 9-11 month the wild Ramulus et folium taxi cuspidatae healthy and strong branch without pest and disease damage
Under, the temperature in this time typically more than 15 DEG C, less than 25 DEG C, this temperature can guarantee that cuttage branch moisture reduce evaporation and
Root heals;The rugosity of cuttage branch is 0.3-0.8cm, a length of 10-15cm, and the branch sheared removes close to incision position 1/3
Blade, then by downward for incision position, taking root liquid soaks 2.5h.
(2) process in seedbed: select perlite, road masonry substrate, a small amount of breeding to can be selected for sandy soil and make substrate, by substrate
Make seedbed, described seedbed width 1.4m, thick 30cm after mixing homogeneously with fertile soil, then water and make nursery soil steady and sure;
(3) cuttage of branch: step (1) soaked branch is inserted seedbed in time, during cuttage, presses standby cutting
Spacing in the rows 5cm, the distance of line-spacing 12cm insert seedbed, and the degree of depth of insertion is the 1/3 of branch length;
(4) final-period management: pour water in time after branch cutting, and on seedbed, make thin film frame, thin film frame terrain clearance is
20cm, thin film spread by thin film frame;The spring of 2-4 month bar of transplanting removes thin film in mid-April, changes sunshade net, the screening of sunshade net
Sun rate is 85%;The autumn sowing branch of the 9-11 month removes thin film at Second Year mid-April, and the sunshade rate changing sunshade net is 85%;
Observe situation of taking root at any time, intercut and keep green with rear blade, the most withered the most yellow, illustrate that management is normal, extracted until 20 days time and check,
Whether cutting base portion otch has healing sign, has cell tissue to grow, and this is the important behaviour before taking root;Spring transplants bar at cell
Tissue increases followed by takes root, after this cell tissue of autumn sowing Seedling increases, and gas after February in the coming year to be waited until after mercury dropped
Just can rush continued growth in original cell tissue after temperature rise to make new advances root;After treating that new root length goes out, impose fertilizer, impose fertilizer
Consumption is that every mu of seedbed imposes 12kg carbamide, 7kg compound fertilizer;Described compound fertilizer is that the weight quota ratio of effective ingredient is, nitrogenous fertilizer:
Phosphate fertilizer: potash fertilizer=4:4:4;Periodically weeding, waters, and can use foliage fertilizer simultaneously, within general 20 days, can spray a foliage fertilizer;2-4
The cutting seedling of the moon can be transplanted or grafting in JIUYUE then, and the cutting seedling of the 9-11 month can be transplanted or transfer at Second Year June
Connect.
The survival rate of the cuttage branch of the present embodiment is 98%.
The cutting seedling of the present embodiment being transplanted, the Second Year height of seedling of transplanting increases as 35cm.
[embodiment 3]
A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding, it comprises the following steps:
(1) process of cuttage branch: cut selecting the 2-4 month or the 9-11 month the wild Ramulus et folium taxi cuspidatae healthy and strong branch without pest and disease damage
Under, the temperature in this time typically more than 15 DEG C, less than 25 DEG C, this temperature can guarantee that cuttage branch moisture reduce evaporation and
Root heals;The rugosity of cuttage branch is 0.3-0.8cm, a length of 10-15cm, and the branch sheared removes close to incision position 1/3
Blade, then by downward for incision position, taking root liquid soaks 2h.
(2) process in seedbed: select perlite, road masonry substrate, a small amount of breeding to can be selected for sandy soil and make substrate, by substrate
Make seedbed, described seedbed width 1.2m, thick 25cm after mixing homogeneously with fertile soil, then water and make nursery soil steady and sure;
(3) cuttage of branch: step (1) soaked branch is inserted seedbed in time, during cuttage, presses standby cutting
Spacing in the rows 4.5cm, the distance of line-spacing 10cm insert seedbed, and the degree of depth of insertion is the 1/3 of branch length;
(4) final-period management: pour water in time after branch cutting, and on seedbed, make thin film frame, thin film frame terrain clearance is
15cm, thin film spread by thin film frame;The spring of 2-4 month bar of transplanting removes thin film in mid-April, changes sunshade net, the screening of sunshade net
Sun rate is 80%;The autumn sowing branch of the 9-11 month removes thin film at Second Year mid-April, and the sunshade rate changing sunshade net is 80%;
Observe situation of taking root at any time, intercut and keep green with rear blade, the most withered the most yellow, illustrate that management is normal, extracted until 20 days time and check,
Whether cutting base portion otch has healing sign, has cell tissue to grow, and this is the important behaviour before taking root;Spring transplants bar at cell
Tissue increases followed by takes root, after this cell tissue of autumn sowing Seedling increases, and gas after February in the coming year to be waited until after mercury dropped
Just can rush continued growth in original cell tissue after temperature rise to make new advances root;After treating that new root length goes out, impose fertilizer, impose fertilizer
Consumption is that every mu of seedbed imposes 11kg carbamide, 6kg compound fertilizer;Described compound fertilizer is that the weight quota ratio of effective ingredient is, nitrogenous fertilizer:
Phosphate fertilizer: potash fertilizer=3:5:5;Periodically weeding, waters, and can use foliage fertilizer simultaneously, within general 20 days, can spray a foliage fertilizer;2-4
The cutting seedling of the moon can be transplanted or grafting in JIUYUE then, and the cutting seedling of the 9-11 month can be transplanted or transfer at Second Year June
Connect.
The survival rate of the cuttage branch of the present embodiment is 95%.
The cutting seedling of the present embodiment being transplanted, the Second Year height of seedling of transplanting increases as 36cm.
[comparative example 1]
This comparative example comprises the following steps:
(1) process of cuttage branch: are selecting the 2-4 month or the 9-11 month Ramulus et folium taxi cuspidatae being good for without pest and disease damage of existing artificial propagation
Vigorous branches bar is cut, and the temperature in this time is typically more than 15 DEG C, less than 25 DEG C, and this temperature can guarantee that cuttage branch moisture subtracts
Few evaporation and root heal;The rugosity of cuttage branch is 0.3-0.8cm, a length of 10-15cm.
(2) process in seedbed: make seedbed, described seedbed width 1.2m, thick 25cm with fertile soil, then water and make Seedling
Bed soil is steady and sure;
(3) cuttage of branch: the branch of step (1) is inserted seedbed in time, during cuttage, by standby cutting by spacing in the rows
4.5cm, the distance of line-spacing 10cm insert seedbed, and the degree of depth of insertion is the 1/3 of branch length;
(4) final-period management: pour water in time after branch cutting;Observe situation of taking root at any time, intercut and keep green with rear blade,
The most withered the most yellow, illustrate that management is normal, extracted until 20 days time and check, whether cutting base portion otch has healing sign, has cell tissue
Growth, this is the important behaviour before taking root;Spring bar of transplanting increases in cell tissue and followed by takes root, this cell tissue of autumn sowing Seedling
After increasing, after mercury dropped, after February in the coming year to be waited until, temperature just can rush continuation life in original cell tissue after raising
The long root that makes new advances;After treating that new root length goes out, imposing fertilizer, imposing fertilizer amount is that every mu of seedbed imposes 11kg carbamide, and 6kg is combined
Fertile;Described compound fertilizer is that the weight quota ratio of effective ingredient is, nitrogenous fertilizer: phosphate fertilizer: potash fertilizer=3:5:5;Periodically weeding, waters, with
Time can use foliage fertilizer, within general 20 days, a foliage fertilizer can be sprayed;The cutting seedling of the 2-4 month can be transplanted or transfer in JIUYUE then
Connecing, the cutting seedling of the 9-11 month can be transplanted or grafting at Second Year June.
The survival rate of the cuttage branch of the present embodiment is 87%.
The cutting seedling of the present embodiment being transplanted, the Second Year height of seedling of transplanting increases as 18cm.
Being illustrated by the data of embodiment 1-3 and comparative example 1, the wild Ramulus et folium taxi cuspidatae of the selection of embodiment 1-3 is anosis
The healthy and strong branch of insect pest has stronger vitality, and the wild Ramulus et folium taxi cuspidatae after taking root liquid processes is without the healthy and strong branch of pest and disease damage
The survival rate of bar is higher than the survival rate of common Ramulus et folium taxi cuspidatae branch.
The cutting seedling of embodiment 1-3 and comparative example 1 being transplanted, after 1 year, the height of seedling of embodiment 1-3 increases remote
Increase higher than the height of seedling of comparative example 1, the Semen Phaseoli selecting the growing way of wild Ramulus et folium taxi cuspidatae branch to be better than existing artificial propagation is described
China fir branch.
It is embodiments of the invention as mentioned above.The present invention is not limited to above-mentioned embodiment, and anyone should learn
The structure change made under the enlightenment of the present invention, every have same or like technical scheme with the present invention, each falls within this
Within the protection domain of invention.
Claims (5)
1. the method for a wild Ramulus et folium taxi cuspidatae branch cutting breeding, it is characterised in that: it comprises the following steps:
(1) selection of cuttage branch and process: select wild Ramulus et folium taxi cuspidatae to cut, then by fracture without the healthy and strong branch of pest and disease damage
1.5-2.5h downwards, soaks in taking root liquid in place;
(2) seedbed is made;
(3) cuttage of branch;
(4) final-period management;
(5) transplanting of cuttage branch or grafting.
The method of a kind of wild Ramulus et folium taxi cuspidatae branch cutting the most according to claim 1 breeding, it is characterised in that: concrete steps
As follows:
(1) process of cuttage branch: are selecting the 2-4 month or the 9-11 month the wild Ramulus et folium taxi cuspidatae healthy and strong branch without pest and disease damage to cut,
The branch sheared removes the blade close to incision position 1/3, then that incision position is downward, soaks 1.5-2.5h in taking root liquid;
(2) process in seedbed: select one or more in perlite, Lu Shi, sandy soil to make substrate, substrate is mixed with fertile soil
Make seedbed, described seedbed width 1-1.4m, thick 20-30cm after closing uniformly, then water and make nursery soil steady and sure;
(3) cuttage of branch: step (1) soaked branch is inserted seedbed in time, during cuttage, by standby cutting by spacing in the rows
4-5cm, the distance of line-spacing 8-12cm insert seedbed, and the degree of depth of insertion is the 1/3 of branch length;
(4) final-period management: pour water in time after branch cutting, and on seedbed, make thin film frame, thin film spread by thin film frame;The 2-4 month
Cutting seedling remove thin film in mid-April, change sunshade net;The cutting seedling of the 9-11 month removes thin film at Second Year mid-April, changes
Upper sunshade net;Observe situation of taking root at any time, after treating that new root length goes out, impose fertilizer;Periodically weeding, waters;The cutting seedling of the 2-4 month
Can transplant or grafting in JIUYUE then, the cutting seedling of the 9-11 month can be transplanted or grafting at Second Year June.
The method of a kind of wild Ramulus et folium taxi cuspidatae branch cutting the most according to claim 2 breeding, it is characterised in that: described step
Suddenly in (4), thin film frame terrain clearance is 10-20cm.
The method of a kind of wild Ramulus et folium taxi cuspidatae branch cutting the most according to claim 2 breeding, it is characterised in that: described step
Suddenly in (4), the sunshade rate of sunshade net is 75-85%.
The method of a kind of wild Ramulus et folium taxi cuspidatae branch cutting the most according to claim 2 breeding, it is characterised in that: described step
Suddenly imposing fertilizer amount in (4) is that every mu of seedbed imposes 10-12kg carbamide, 5-7kg compound fertilizer;Described compound fertilizer is effective ingredient
Weight quota ratio be, nitrogenous fertilizer: phosphate fertilizer: potash fertilizer=3-4:4-5:4-5.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107371987A (en) * | 2017-09-12 | 2017-11-24 | 贵州阳溪洪林中药材产业开发有限公司 | A kind of Yew cuttage breeding and seedling method |
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CN102960174A (en) * | 2012-12-11 | 2013-03-13 | 雷学军 | Method for breeding and cultivating Taxus chinensis var mairei through asexual cuttage |
CN103947437A (en) * | 2014-05-13 | 2014-07-30 | 兴文县康永红豆杉生物科技有限责任公司 | Taxus X media non-test tube rapid propagation method |
CN103975737A (en) * | 2014-05-16 | 2014-08-13 | 四川农业大学 | Chinese yew vegetative propagation method |
CN105123249A (en) * | 2015-09-28 | 2015-12-09 | 桐城市红豆杉生态农林科技有限公司 | Cutting and breeding method for taxus chinensis |
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2016
- 2016-07-19 CN CN201610569050.0A patent/CN106171762A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102960174A (en) * | 2012-12-11 | 2013-03-13 | 雷学军 | Method for breeding and cultivating Taxus chinensis var mairei through asexual cuttage |
CN103947437A (en) * | 2014-05-13 | 2014-07-30 | 兴文县康永红豆杉生物科技有限责任公司 | Taxus X media non-test tube rapid propagation method |
CN103975737A (en) * | 2014-05-16 | 2014-08-13 | 四川农业大学 | Chinese yew vegetative propagation method |
CN105123249A (en) * | 2015-09-28 | 2015-12-09 | 桐城市红豆杉生态农林科技有限公司 | Cutting and breeding method for taxus chinensis |
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CN107371987A (en) * | 2017-09-12 | 2017-11-24 | 贵州阳溪洪林中药材产业开发有限公司 | A kind of Yew cuttage breeding and seedling method |
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Application publication date: 20161207 |