CN105123249A - Cutting and breeding method for taxus chinensis - Google Patents

Cutting and breeding method for taxus chinensis Download PDF

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Publication number
CN105123249A
CN105123249A CN201510628472.6A CN201510628472A CN105123249A CN 105123249 A CN105123249 A CN 105123249A CN 201510628472 A CN201510628472 A CN 201510628472A CN 105123249 A CN105123249 A CN 105123249A
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seedling
cuttage
root
fringe bar
seedbed
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郭黄胜
钱候春
代会
李决发
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Tongcheng Hongdoushan Ecological Agriculture And Forest Technology Co Ltd
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Tongcheng Hongdoushan Ecological Agriculture And Forest Technology Co Ltd
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Publication of CN105123249A publication Critical patent/CN105123249A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a cutting and breeding method for taxus chinensis, and belongs to the technical field of plant breeding. The cutting and breeding method comprises the following steps: scion wood selection, scion wood treatment, scion wood cutting, management after cutting, seedling transplantation, and management after transplantation. The adopted cutting and breeding method has the advantages that the cutting and breeding method with high survival rate is provided for taxus chinensis breeding in the south, the planting range of taxus chinensis is enlarged, and the difficult problem of taxus chinensis resource shortage can be favorably solved.

Description

The cuttage and seedling culture method of a kind of Chinese yew
Technical field
The present invention relates to the cuttage and seedling culture method of a kind of Chinese yew, belong to technical field of plant cultivation.
Background technology
Chinese yew, genus shallow rooted plant, its main root is not obvious, side root flourishing, shade-tolerant tree species, the weather of happiness warm and moist, is usually grown on comparatively moist place, innerland, the foot of the hill.Self-sow is in the abundant acid ground of the mountain valley of height above sea level less than 1000 meters or 1500 meters, small stream limit, gentle slope humus, and require the yellow earth that fertility is higher, yellowish soil, neutral soil, calcium soil also can grow.Drought-resistant barren, not resistance to low-lying ponding.Comparatively strong to climatic adaptation power, year samming 11-16 DEG C, minimum extreme value can reach-11 DEG C.Have stronger rudiment ability, common rudiment sprig on trunk, because growth of taxol speed is slow under field conditions (factors), regeneration capacity is poor.China has been classified as one-level rare endangered plants, and the United Nations also prohibites felling.The endangered natural rare plants for anticancer of generally acknowledging in the world, the ancient seeds that quaternary glacier carries over, the on earth history of existing 2,500,000 years.
Because Chinese yew has higher medical value and ornamental value, cut down with causing global a large amount of original wild being ransacked of property of Chinese yew, or full tree bares a tree of its bark, resource is increasingly deficient.In addition Chinese yew dioecism, how female hero is less, percentage of seedgermination is low, poor growth, a lot of biological property limits the development of Natural Population, more exacerbates the circumstances that it is in imminent danger.Therefore only have and adopt artificial measures to carry out cultivating (cuttage), strengthen introducing and cultivating technology research, to expand total resources, expand its planting range, this problem of resource scarcity could be solved better.
Summary of the invention
The object of the invention is to: the cuttage and seedling culture method that the Chinese yew that a kind of survival rate is high is provided.
In order to realize foregoing invention object, the invention provides following technical scheme:
A cuttage and seedling culture method for Chinese yew, comprises the following steps: the process of the selecting of fringe bar, fringe bar, the cuttage of fringe bar, insert after management, seedling replanting, manage after cultivation.
Preferably, the cuttage and seedling culture method of described Chinese yew, comprises the following steps:
(1) the selecting of fringe bar
Semi-lignified branch or the branch from root sprouting make fringe bar then in the tree crown middle and lower part of clip 4 ~ 8 years breeder mother trees;
(2) process of fringe bar
The fringe bar clear water cut is put into carbendazim solution after cleaning and is carried out sterilizing, disinfecting time is 5 minutes, then carry out cutting fringe, fringe bar cuts by 10 ㎝ ~ 15cm length and makes cuttings, cuts off whole branches and leaves of less than 2/3, retain 3 ~ 5 axillalry buds or side terminal bud before inserting, otch smoothly, not broken skin, not splitting, lower end is cut into horse ear shape, and immersed by fringe bar in 200mg/L indolebutyric acid liquid and soak 2h, soaking depth is 4.8-5.2cm;
(3) cuttage of fringe bar
Cuttage in October early and middle ten days carry out, the wide 1.25m in seedbed, height of bed 40cm, the cuttage degree of depth 5 ~ 8cm, distance between rows and hills 4cm × 5cm, seedbed film arch cover moisturizing, built on seedbed, with Shading net covering;
(4) management after inserting
After inserting, every day sprays water 3 ~ 4 times, and after two weeks, every day sprays water 1 ~ 2 time; 40d before after inserting, temperature controls at 20-25 DEG C, and relative moisture is at 75-85%, and illumination controls at 1700-2000lx, sprays 50% carbendazol wettable powder every 10d, pre-preventing germs infection;
After inserting, 35-45d produces callus, and before taking root, remove the sprouting of cuttings in time, do not make cuttings activity in order to avoid injure established callus during bud picking, impact is taken root; After inserting, 50-80d has a small amount of root to generate, and most of cuttings sprays rooting type fertilizer every 10-15d after producing callus; 90-110d is taken root in a large number, reserve part rudiment, will remove susceptible and rotten cuttings in time in daily management, in order to avoid infect other cuttings;
(5) seedling replanting
When annual height of seedling 15 ~ 35cm, the thick 0.3cm of leading thread, side shoot 3 ~ 5, transplant, root system should be kept complete, do not damage terminal bud during transplanting, not damaged root skin, forbid to use hand-lifting seedling, the lifting degree of depth looks seedling root upgrowth situation and different, must not be less than 20cm;
(6) management after planting
Erection cool canopy: shade with sunshade net: more than net height > 2.3m;
Water management: after little transplantation of seedlings, pours water according to soil dry-wet situation, and accomplishes late filling early row, makes nursery remain moisture state, wants timely draining when rainy season or milpa ponding, especially seedling period, prevents field ponding;
Fertilizing management: seedling growth is executed once with the aqueous solution of urea of 0.2% every January early stage; Middle and later periods uses the nutrient solution of specially joining every January; Little transplantation of seedlings, after 20 days, coordinates intertill and clean tillage, monthly waters a liquid manure, first rare rear dense; Every mu with urea 3 ~ 10kg, potassium chloride 1 ~ 5kg water mixing water execute or diluted after do foliage fertilizer spraying, be as the criterion to squirt seedbed;
Intertill and clean tillage: little transplantation of seedlings, after 20 days, will carry out 2 ~ 3 intertill and clean tillages before rainy season in early spring and after the rainy day in autumn; Shallow hoe is wanted in intertillage, does not hinder shoot root, and ditch earth culture on seedbed;
Thinning and final singling: carry out thinning and final singling by the requirement of distance between rows and hills 15cm × 25cm, pull out overstocked, the undergrowth of growth and seedling that is injured, that infect damage by disease and insect, seedling be evenly distributed, meanwhile, carry out benefits cultivation to too sparse location;
The extermination of disease and insect pest: enrich potassium, phosphate fertilizer and ash seedling stage, promotes seedling growth, to strengthen the premunition of seedling; Control nursery environment, accomplish in garden without weeds; Protectorate cultivation will strengthen ventilation dewetting, and reduce the moisture in air humidity and soil, outdoor cropping will build high ridge, holds drain ditch successfully, removes the ponding in milpa in time; Pulverized limestone mixes with the ratio of 1:4 with ash by nursery stock children's tender period, every mu of seedbed spread fertilizer over the fields l00 ~ 150kg or in the seeding stage every 7 days, with the bordeaux mixture sprinkling seedling of 0.5%; When finding disease seedling and diseased plant, carbendazim, topsin, jinggangmycin clean with hundred bacterium dilute 800 times ~ 1000 times liquid sprays and control, every spray in 2 ~ 3 days once, and continuous spraying 2 ~ 3 times.
Preferably, in step (3), the bottom in seedbed is covered with thickness is 13-17cm green tea produced in Anhui Province stones, and green tea produced in Anhui Province stone repaving thickness is the mixture that 20 ~ 25cm river sand adds turfy soil, and wherein the mass ratio of river sand and turfy soil is 3:7.
Preferably, the formula of the nutrient solution of specially joining used in the fertilizing management of described step (6) is: nitrate of lime 550mg/L, potassium nitrate 150mg/L, magnesium sulfate 155mg/L, potassium dihydrogen phosphate 135mg/L, potassium chloride 750mg/L, ammonium sulfate 750mg/L, manganese sulphate 0.40mg/L, zinc sulphate 0.06mg/L, boric acid 0.65mg/L, sodium molybdate 0.025mg/L, chelated iron 0.65mg/L.
Preferably, the thinning in described step (6) and final singling management kind of final singling select overcast and rainy carrying out with mending to plant.
Beneficial effect of the present invention is:
The present invention is that Chinese yew provides the high cuttage and seedling culture method of a kind of survival rate in southern cultivation, expands the planting range of Chinese yew, is conducive to solving a taxus resource difficult problem in short supply.
Embodiment:
A cuttage and seedling culture method for Chinese yew, comprises the following steps:
(1) the selecting of fringe bar
Semi-lignified branch or the branch from root sprouting make fringe bar then in the tree crown middle and lower part of clip 4 ~ 8 years breeder mother trees;
(2) process of fringe bar
The fringe bar clear water cut is put into carbendazim solution after cleaning and is carried out sterilizing, disinfecting time is 5 minutes, then carry out cutting fringe, fringe bar cuts by 12cm length and makes cuttings, cuts off whole branches and leaves of less than 2/3, retain 3 ~ 5 axillalry buds or side terminal bud before inserting, otch smoothly, not broken skin, not splitting, lower end is cut into horse ear shape, and immersed by fringe bar in 200mg/L indolebutyric acid liquid and soak 2h, soaking depth is 4.8-5.2cm;
(3) cuttage of fringe bar
Cuttage in October early and middle ten days carry out, the wide 1.25m in seedbed, height of bed 40cm, the cuttage degree of depth 5 ~ 8cm, distance between rows and hills 4cm × 5cm, seedbed film arch cover moisturizing, built on seedbed, with Shading net covering;
(4) management after inserting
After inserting, every day sprays water 3 ~ 4 times, and after two weeks, every day sprays water 1 ~ 2 time; 40d before after inserting, temperature controls at 20-25 DEG C, and relative moisture is at 75-85%, and illumination controls at 1700-2000lx, sprays 50% carbendazol wettable powder every 10d, pre-preventing germs infection;
After inserting, 35-45d produces callus, and before taking root, remove the sprouting of cuttings in time, do not make cuttings activity in order to avoid injure established callus during bud picking, impact is taken root; After inserting, 50-80d has a small amount of root to generate, and most of cuttings sprays rooting type fertilizer every 10-15d after producing callus; 90-110d is taken root in a large number, reserve part rudiment, will remove susceptible and rotten cuttings in time in daily management, in order to avoid infect other cuttings;
(5) seedling replanting
When annual height of seedling 15 ~ 35cm, the thick 0.3cm of leading thread, side shoot 3 ~ 5, transplant, root system should be kept complete, do not damage terminal bud during transplanting, not damaged root skin, forbid to use hand-lifting seedling, the lifting degree of depth looks seedling root upgrowth situation and different, must not be less than 20cm;
(6) management after planting
Erection cool canopy: shade with sunshade net: more than net height > 2.3m;
Water management: after little transplantation of seedlings, pours water according to soil dry-wet situation, and accomplishes late filling early row, makes nursery remain moisture state, wants timely draining when rainy season or milpa ponding, especially seedling period, prevents field ponding;
Fertilizing management: seedling growth is executed once with the aqueous solution of urea of 0.2% every January early stage; Middle and later periods uses the nutrient solution of specially joining every January; Little transplantation of seedlings, after 20 days, coordinates intertill and clean tillage, monthly waters a liquid manure, first rare rear dense; Every mu with urea 3 ~ 10kg, potassium chloride 1 ~ 5kg water mixing water execute or diluted after do foliage fertilizer spraying, be as the criterion to squirt seedbed;
Intertill and clean tillage: little transplantation of seedlings, after 20 days, will carry out 2 ~ 3 intertill and clean tillages before rainy season in early spring and after the rainy day in autumn; Shallow hoe is wanted in intertillage, does not hinder shoot root, and ditch earth culture on seedbed;
Thinning and final singling: carry out thinning and final singling by the requirement of distance between rows and hills 15cm × 25cm, pull out overstocked, the undergrowth of growth and seedling that is injured, that infect damage by disease and insect, seedling be evenly distributed, meanwhile, carry out benefits cultivation to too sparse location;
The extermination of disease and insect pest: enrich potassium, phosphate fertilizer and ash seedling stage, promotes seedling growth, to strengthen the premunition of seedling; Control nursery environment, accomplish in garden without weeds; Protectorate cultivation will strengthen ventilation dewetting, and reduce the moisture in air humidity and soil, outdoor cropping will build high ridge, holds drain ditch successfully, removes the ponding in milpa in time; Pulverized limestone mixes with the ratio of 1:4 with ash by nursery stock children's tender period, every mu of seedbed spread fertilizer over the fields l00 ~ 150kg or in the seeding stage every 7 days, with the bordeaux mixture sprinkling seedling of 0.5%; When finding disease seedling and diseased plant, carbendazim, topsin, jinggangmycin clean with hundred bacterium dilute 800 times ~ 1000 times liquid sprays and control, every spray in 2 ~ 3 days once, and continuous spraying 2 ~ 3 times.
Further, in step (3), the bottom in seedbed is covered with thickness is 13-17cm green tea produced in Anhui Province stones, and green tea produced in Anhui Province stone repaving thickness is the mixture that 20 ~ 25cm river sand adds turfy soil, and wherein the mass ratio of river sand and turfy soil is 3:7.
Further, the formula of the nutrient solution of specially joining used in the fertilizing management of described step (6) is: nitrate of lime 550mg/L, potassium nitrate 150mg/L, magnesium sulfate 155mg/L, potassium dihydrogen phosphate 135mg/L, potassium chloride 750mg/L, ammonium sulfate 750mg/L, manganese sulphate 0.40mg/L, zinc sulphate 0.06mg/L, boric acid 0.65mg/L, sodium molybdate 0.025mg/L, chelated iron 0.65mg/L.The trace element required for plant can be supplemented.
Further, the thinning in described step (6) and final singling seedling administrative time select overcast and rainy carrying out with mending to plant.Due to overcast and rainy high humidity, plant transpiration amount can be suppressed, improve to mend and plant survival rate.

Claims (5)

1. a cuttage and seedling culture method for Chinese yew, is characterized in that, comprises the following steps: the process of the selecting of fringe bar, fringe bar, the cuttage of fringe bar, insert after management, seedling replanting, manage after cultivation.
2. the cuttage and seedling culture method of Chinese yew according to claim 1, is characterized in that, comprises the following steps:
(1) the selecting of fringe bar
Semi-lignified branch or the branch from root sprouting make fringe bar then in the tree crown middle and lower part of clip 4 ~ 8 years breeder mother trees;
(2) process of fringe bar
The fringe bar clear water cut is put into carbendazim solution after cleaning and is carried out sterilizing, disinfecting time is 5 minutes, then carry out cutting fringe, fringe bar cuts by 10 ㎝ ~ 15cm length and makes cuttings, cuts off whole branches and leaves of less than 2/3, retain 3 ~ 5 axillalry buds or side terminal bud before inserting, otch smoothly, not broken skin, not splitting, lower end is cut into horse ear shape, and immersed by fringe bar in 200mg/L indolebutyric acid liquid and soak 2h, soaking depth is 4.8-5.2cm;
(3) cuttage of fringe bar
Cuttage in early October-mid-November carries out, the wide 1.25m in seedbed, height of bed 40cm, the cuttage degree of depth 5 ~ 8cm, distance between rows and hills 4cm × 5cm, seedbed film arch cover moisturizing, built on seedbed, with Shading net covering;
(4) management after inserting
After inserting, every day sprays water 3 ~ 4 times, and after two weeks, every day sprays water 1 ~ 2 time; 40d before after inserting, temperature controls at 20-25 DEG C, and relative moisture is 80%, and illumination controls at 1700-2000lx, sprays 50% carbendazol wettable powder every 10d, pre-preventing germs infection;
After inserting, 35-45d produces callus, and before taking root, remove the sprouting of cuttings in time, do not make cuttings activity in order to avoid injure established callus during bud picking, impact is taken root; After inserting, 50-80d has a small amount of root to generate, and most of cuttings sprays rooting type fertilizer every 10-15d after producing callus; 90-110d is taken root in a large number, reserve part rudiment, will remove susceptible and rotten cuttings in time in daily management, in order to avoid infect other cuttings;
(5) seedling replanting
When annual height of seedling 15 ~ 35cm, the thick 0.3cm of leading thread, side shoot 3 ~ 5, transplant, root system should be kept complete, do not damage terminal bud during transplanting, not damaged root skin, forbid to use hand-lifting seedling, the lifting degree of depth looks seedling root upgrowth situation and different, must not be less than 20cm;
(6) management after planting
Erection cool canopy: shade with sunshade net: more than net height > 2.3m;
Water management: after little transplantation of seedlings, pours water according to soil dry-wet situation, and accomplishes late filling early row, makes nursery remain moisture state, wants timely draining when rainy season or milpa ponding, especially seedling period, prevents field ponding;
Fertilizing management: seedling growth is executed once with the aqueous solution of urea of 0.2% every January early stage; Middle and later periods uses the nutrient solution of specially joining every January; Little transplantation of seedlings, after 20 days, coordinates intertill and clean tillage, monthly waters a liquid manure, first rare rear dense; Every mu with urea 3 ~ 10kg, potassium chloride 1 ~ 5kg water mixing water execute or diluted after do foliage fertilizer spraying, be as the criterion to squirt seedbed;
Intertill and clean tillage: little transplantation of seedlings, after 20 days, will carry out 2 ~ 3 intertill and clean tillages before rainy season in early spring and after the rainy day in autumn; Shallow hoe is wanted in intertillage, does not hinder shoot root, and ditch earth culture on seedbed;
Thinning and final singling: carry out thinning and final singling by the requirement of distance between rows and hills 15cm × 25cm, pull out overstocked, the undergrowth of growth and seedling that is injured, that infect damage by disease and insect, seedling be evenly distributed, meanwhile, carry out benefits cultivation to too sparse location;
The extermination of disease and insect pest: enrich potassium, phosphate fertilizer and ash seedling stage, promotes seedling growth, to strengthen the premunition of seedling; Control nursery environment, accomplish in garden without weeds; Protectorate cultivation will strengthen ventilation dewetting, and reduce the moisture in air humidity and soil, outdoor cropping will build high ridge, holds drain ditch successfully, removes the ponding in milpa in time; Pulverized limestone mixes with the ratio of 1:4 with ash by nursery stock children's tender period, every mu of seedbed spread fertilizer over the fields l00 ~ 150kg or in the seeding stage every 7 days, with the bordeaux mixture sprinkling seedling of 0.5%; When finding disease seedling and diseased plant, carbendazim, topsin, jinggangmycin clean with hundred bacterium dilute 800 times ~ 1000 times liquid sprays and control, every spray in 2 ~ 3 days once, and continuous spraying 2 ~ 3 times.
3. the cuttage and seedling culture method of Chinese yew according to claim 2, it is characterized in that, in step (3), the bottom in seedbed is covered with thickness is 13-17cm green tea produced in Anhui Province stones, green tea produced in Anhui Province stone repaving thickness is the mixture that 20 ~ 25cm river sand adds turfy soil, and wherein the mass ratio of river sand and turfy soil is 3:7.
4. the cuttage and seedling culture method of Chinese yew according to claim 2, it is characterized in that, the formula of the nutrient solution of specially joining used in the fertilizing management of described step (6) is: nitrate of lime 550mg/L, potassium nitrate 150mg/L, magnesium sulfate 155mg/L, potassium dihydrogen phosphate 135mg/L, potassium chloride 750mg/L, ammonium sulfate 750mg/L, manganese sulphate 0.40mg/L, zinc sulphate 0.06mg/L, boric acid 0.65mg/L, sodium molybdate 0.025mg/L, chelated iron 0.65mg/L.
5. the cuttage and seedling culture method of Chinese yew according to claim 2, is characterized in that, during the thinning in described step (6) and final singling manage, final singling and benefit are planted and selected overcast and rainy carrying out.
CN201510628472.6A 2015-09-28 2015-09-28 Cutting and breeding method for taxus chinensis Pending CN105123249A (en)

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Cited By (11)

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Publication number Priority date Publication date Assignee Title
CN105475080A (en) * 2016-01-27 2016-04-13 苏州星火园艺科技开发中心 Dawn redwood planting method
CN106034712A (en) * 2016-06-30 2016-10-26 四川省林业科学研究院 Alder twig single-bud cuttage propagation method
CN106171762A (en) * 2016-07-19 2016-12-07 泸州千秋辉煌农业有限公司 A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding
CN106416562A (en) * 2016-08-31 2017-02-22 刘全 Comprehensive economic forest tending method
CN107155770A (en) * 2017-05-26 2017-09-15 四川祥光农业科技开发有限公司 A kind of cuttage and seedling culture method of Taxus x media
CN107548876A (en) * 2017-10-13 2018-01-09 和县常久农业发展有限公司 A kind of efficient implantation methods of grape
CN108684345A (en) * 2017-03-03 2018-10-23 黄振忠 A kind of Chloranthus glaber cuttage and seedling culture method
CN108811821A (en) * 2018-07-26 2018-11-16 曲阜师范大学 A method of promote ornamental plant green wood cutting to take root
CN109168691A (en) * 2018-10-29 2019-01-11 宜宾云辰乔木园林有限责任公司 A method of promoting silver-colored China fir cuttage root-taking
CN111296090A (en) * 2020-03-27 2020-06-19 华南农业大学 Cutting propagation method of Chinese cinnamon and application thereof
CN111802099A (en) * 2020-07-31 2020-10-23 陇南隆康农业科技有限公司 Taxus chinensis seedling breeding method

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105475080A (en) * 2016-01-27 2016-04-13 苏州星火园艺科技开发中心 Dawn redwood planting method
CN106034712B (en) * 2016-06-30 2019-04-19 四川省林业科学研究院 A kind of alder spray single-bud cuttage method for culturing seedlings
CN106034712A (en) * 2016-06-30 2016-10-26 四川省林业科学研究院 Alder twig single-bud cuttage propagation method
CN106171762A (en) * 2016-07-19 2016-12-07 泸州千秋辉煌农业有限公司 A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding
CN106416562A (en) * 2016-08-31 2017-02-22 刘全 Comprehensive economic forest tending method
CN106416562B (en) * 2016-08-31 2017-10-10 刘全 A kind of synthesis Tending methods of economic forest
CN108684345A (en) * 2017-03-03 2018-10-23 黄振忠 A kind of Chloranthus glaber cuttage and seedling culture method
CN107155770A (en) * 2017-05-26 2017-09-15 四川祥光农业科技开发有限公司 A kind of cuttage and seedling culture method of Taxus x media
CN107548876A (en) * 2017-10-13 2018-01-09 和县常久农业发展有限公司 A kind of efficient implantation methods of grape
CN108811821A (en) * 2018-07-26 2018-11-16 曲阜师范大学 A method of promote ornamental plant green wood cutting to take root
CN109168691A (en) * 2018-10-29 2019-01-11 宜宾云辰乔木园林有限责任公司 A method of promoting silver-colored China fir cuttage root-taking
CN111296090A (en) * 2020-03-27 2020-06-19 华南农业大学 Cutting propagation method of Chinese cinnamon and application thereof
CN111296090B (en) * 2020-03-27 2021-08-31 华南农业大学 A kind of cutting propagation method of Chinese cinnamon and its application
CN111802099A (en) * 2020-07-31 2020-10-23 陇南隆康农业科技有限公司 Taxus chinensis seedling breeding method

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