CN106069765A - A kind of tissue method for culturing seedlings of organic cauliflower - Google Patents
A kind of tissue method for culturing seedlings of organic cauliflower Download PDFInfo
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- CN106069765A CN106069765A CN201610491840.1A CN201610491840A CN106069765A CN 106069765 A CN106069765 A CN 106069765A CN 201610491840 A CN201610491840 A CN 201610491840A CN 106069765 A CN106069765 A CN 106069765A
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- tissue
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- cauliflower
- plantation
- culturing seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses the tissue method for culturing seedlings of a kind of organic cauliflower, including tissue sampling, organized processing, nutritive cube plantation, seedling management and the several process of transplanting.The present invention has the advantage that in the present invention that the short stem utilizing organic cauliflower is cultivated compared to existing technology, it is possible to effectively keep the fine quality of organic cauliflower；Short stem tissue is processed, can not only effectively sterilize, moreover it is possible to effective activation histiocyte；Cover composite soil after plantation, while sterilization, promote short stem-root；Strict control illumination and intensity of illumination, it is to avoid tissue infection so that it is fast-growth；Transplanting after bearing adventitious root, survival rate is up to more than 93.6%.
Description
Technical field
The invention belongs to technical field of agricultural breeding, be specifically related to the tissue method for culturing seedlings of a kind of organic cauliflower.
Background technology
Along with being continually changing of the market demand, bouquet loose type cauliflower i.e. organic cauliflower, with its light green bennet, crisp
Sweet quality is liked by consumers in general, and increasing growing vegetables base starts to select colory seed kind to cultivate
Organic cauliflower, but survival rate is relatively low, and the most affected by environment in cultivating process, after nursery, survival rate is low, increases planting cost,
It is thus desirable to study a kind of method for culturing seedlings for organic Herba Astragali Sinici, improve its survival rate.
Summary of the invention
It is an object of the invention to for existing problem, it is provided that the tissue method for culturing seedlings of a kind of organic cauliflower.
The present invention is achieved by the following technical solutions: the tissue method for culturing seedlings of a kind of organic cauliflower, including following step
Rapid:
(1) tissue sampling: select mature flower Broccoli best in quality, that cauliflower top layer is neat, intercepts under bouquet after removing bouquet
Short stem 1-1.5cm, standby；
(2) organized processing: being processed by the tissue mixed liquid dipping gathered, treatment temperature is 35-40 DEG C, and soak time is 1.5-
2 hours, then use deionized water rinsing 3-4 time, use sterile gauze suck dry moisture, standby；
Wherein, mixed liquor includes the raw material of following percentage by weight: carbendazim 15-20ppm, copper chloride 40-50ppm, permanganic acid
Potassium 0.2-0.5%, indolepopionic acid potassium 20-30ppm, sodium hydroxide 5-6%, surplus is water；
(3) nutritive cube plantation: treated tissue, built with the seedling medium of 70%, is inserted in seedling medium, covers by nutritive cube
Composite soil 0.5cm, composite soil is by fine earth and reclaims Folium Camelliae sinensis dry powder blend and forms, and mixed weight ratio is for 4:1；
(4) seedling management: after plantation in 1-5 days, keeps illumination 18 hours, and intensity of illumination is 2600-2800lx；6-after plantation
15 days, keeping illumination 16 hours, intensity of illumination is 2400-2500lx；Arrive after 15 days before transplanting, keep illumination 14 little
Time, intensity of illumination is 2000-2200lx；
(5) transplant: in plantation latter about 30 days, check that seedling root bears many adventitious roots, can transplant.
As further improvement of these options, the grain diameter of described composite soil is 20-30 μm, and water content is 32-
35%。
As further improvement of these options, adding modifying agent in described seedling medium, the addition of modifying agent is
5-8%；
Modifying agent is made up of the raw material of following weight portion: sepiolite powder 15-20 part, feldspar in powder 12-18 part, Petiolus Trachycarpi oil 2-3 part,
Ammonium dihydrogen phosphate 8-15 part, Robinia pseudoacacia L. leaf powder 15-20 part, weathered coal 5-8 part, stevioside 4-6 part, carbamide 10-15 part, barium silicate
2-4 part, biological bacteria 5-8 part；
The preparation method of modifying agent is: remaining material mixing in addition to Petiolus Trachycarpi oil and stevioside is uniform, adds suitable quantity of water so that it is
Water content is 35-40%, under conditions of 40-50 DEG C, seals and stacks 2-3 days, be down to room temperature after completing, and adds Petiolus Trachycarpi oil and sweet
Inulin, stirs, and dries, pelletize and get final product.
As further improvement of these options, described biological bacteria is bacillus subtilis, and its viable count is 6 × 108-
8×108 cfu/g。
As further improvement of these options, the grain diameter of described modifying agent is 0.3-0.5mm, and water content is 8-
12%。
Seedling medium adds appropriate modifying agent, improves the ability of substrate fertilizer and fertilizer conservation, coordinate strict illumination control
System, can effectively facilitate the growth of short stem-root.
The present invention has the advantage that in the present invention that the short stem utilizing organic cauliflower is cultivated compared to existing technology, energy
Enough fine qualities effectively keeping organic cauliflower；Short stem tissue is processed, can not only effectively sterilize, moreover it is possible to effective activation
Histiocyte；Cover composite soil after plantation, while sterilization, promote short stem-root；Strict control illumination and intensity of illumination, it is to avoid
Tissue infection so that it is fast-growth；Transplanting after bearing adventitious root, survival rate is up to more than 93.6%.
Detailed description of the invention
Embodiment 1
The tissue method for culturing seedlings of a kind of organic cauliflower, comprises the following steps:
(1) tissue sampling: select mature flower Broccoli best in quality, that cauliflower top layer is neat, intercepts under bouquet after removing bouquet
Short stem 1-1.5cm, standby；
(2) organized processing: being processed by the tissue mixed liquid dipping gathered, treatment temperature is 35-40 DEG C, and soak time is 1.5-
2 hours, then use deionized water rinsing 3-4 time, use sterile gauze suck dry moisture, standby；
Wherein, mixed liquor includes the raw material of following percentage by weight: carbendazim 15-20ppm, copper chloride 40-50ppm, permanganic acid
Potassium 0.2-0.5%, indolepopionic acid potassium 20-30ppm, sodium hydroxide 5-6%, surplus is water；
(3) nutritive cube plantation: treated tissue, built with the seedling medium of 70%, is inserted in seedling medium, covers by nutritive cube
Composite soil 0.5cm, composite soil is by fine earth and reclaims Folium Camelliae sinensis dry powder blend and forms, and mixed weight ratio is for 4:1；
(4) seedling management: after plantation in 1-5 days, keeps illumination 18 hours, and intensity of illumination is 2600-2800lx；6-after plantation
15 days, keeping illumination 16 hours, intensity of illumination is 2400-2500lx；Arrive after 15 days before transplanting, keep illumination 14 little
Time, intensity of illumination is 2000-2200lx；
(5) transplant: in plantation latter about 30 days, check that seedling root bears many adventitious roots, can transplant.
Wherein, the grain diameter of described composite soil is 20-30 μm, and water content is 32-35%.
Wherein, adding modifying agent in described seedling medium, the addition of modifying agent is 5-8%；
Modifying agent is made up of the raw material of following weight portion: sepiolite powder 15-20 part, feldspar in powder 12-18 part, Petiolus Trachycarpi oil 2-3 part,
Ammonium dihydrogen phosphate 8-15 part, Robinia pseudoacacia L. leaf powder 15-20 part, weathered coal 5-8 part, stevioside 4-6 part, carbamide 10-15 part, barium silicate
2-4 part, biological bacteria 5-8 part；
The preparation method of modifying agent is: remaining material mixing in addition to Petiolus Trachycarpi oil and stevioside is uniform, adds suitable quantity of water so that it is
Water content is 35-40%, under conditions of 40-50 DEG C, seals and stacks 2-3 days, be down to room temperature after completing, and adds Petiolus Trachycarpi oil and sweet
Inulin, stirs, and dries, pelletize and get final product.
Wherein, described biological bacteria is bacillus subtilis, and its viable count is 6 × 108-8×108 cfu/g；Described modifying agent
Grain diameter be 0.3-0.5mm, water content is 8-12%.
Being managed collectively nursery in the present invention, survival rate of seedling is higher than 93.6%, and after transplanting, survival rate is high, and plantation gained is organic
Cauliflower can keep advantageous property, and cauliflower shape is loose, and alabastrum surface is uniformly neat, and bennet is light green, and edible have crisp sweet mouthfeel, business
Moral character is preferable.
Claims (5)
1. the tissue method for culturing seedlings of an organic cauliflower, it is characterised in that comprise the following steps:
(1) tissue sampling: select mature flower Broccoli best in quality, that cauliflower top layer is neat, intercepts under bouquet after removing bouquet
Short stem 1-1.5cm, standby；
(2) organized processing: being processed by the tissue mixed liquid dipping gathered, treatment temperature is 35-40 DEG C, and soak time is 1.5-
2 hours, then use deionized water rinsing 3-4 time, use sterile gauze suck dry moisture, standby；
Wherein, mixed liquor includes the raw material of following percentage by weight: carbendazim 15-20ppm, copper chloride 40-50ppm, permanganic acid
Potassium 0.2-0.5%, indolepopionic acid potassium 20-30ppm, sodium hydroxide 5-6%, surplus is water；
(3) nutritive cube plantation: treated tissue, built with the seedling medium of 70%, is inserted in seedling medium, covers by nutritive cube
Composite soil 0.5cm, composite soil is by fine earth and reclaims Folium Camelliae sinensis dry powder blend and forms, and mixed weight ratio is for 4:1；
(4) seedling management: after plantation in 1-5 days, keeps illumination 18 hours, and intensity of illumination is 2600-2800lx；6-after plantation
15 days, keeping illumination 16 hours, intensity of illumination is 2400-2500lx；Arrive after 15 days before transplanting, keep illumination 14 little
Time, intensity of illumination is 2000-2200lx；
(5) transplant: in plantation latter about 30 days, check that seedling root bears many adventitious roots, can transplant.
The tissue method for culturing seedlings of a kind of organic cauliflower the most as claimed in claim 1, it is characterised in that the particle of described composite soil
Footpath is 20-30 μm, and water content is 32-35%.
The tissue method for culturing seedlings of a kind of organic cauliflower the most as claimed in claim 1, it is characterised in that add in described seedling medium
Modifying agent, the addition of modifying agent is 5-8%；
Modifying agent is made up of the raw material of following weight portion: sepiolite powder 15-20 part, feldspar in powder 12-18 part, Petiolus Trachycarpi oil 2-3 part,
Ammonium dihydrogen phosphate 8-15 part, Robinia pseudoacacia L. leaf powder 15-20 part, weathered coal 5-8 part, stevioside 4-6 part, carbamide 10-15 part, barium silicate
2-4 part, biological bacteria 5-8 part；
The preparation method of modifying agent is: remaining material mixing in addition to Petiolus Trachycarpi oil and stevioside is uniform, adds suitable quantity of water so that it is
Water content is 35-40%, under conditions of 40-50 DEG C, seals and stacks 2-3 days, be down to room temperature after completing, and adds Petiolus Trachycarpi oil and sweet
Inulin, stirs, and dries, pelletize and get final product.
The tissue method for culturing seedlings of a kind of organic cauliflower the most as claimed in claim 3, it is characterised in that described biological bacteria is hay bud
Spore bacillus, its viable count is 6 × 108-8×108 cfu/g。
The tissue method for culturing seedlings of a kind of organic cauliflower the most as claimed in claim 3, it is characterised in that the particle of described modifying agent
Footpath is 0.3-0.5mm, and water content is 8-12%.
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|---|---|---|---|
| CN201610491840.1A CN106069765B (en) | 2016-06-29 | 2016-06-29 | A kind of tissue method for culturing seedlings of cauliflower |
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|---|---|---|---|
| CN201610491840.1A CN106069765B (en) | 2016-06-29 | 2016-06-29 | A kind of tissue method for culturing seedlings of cauliflower |
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| CN106069765A true CN106069765A (en) | 2016-11-09 |
| CN106069765B CN106069765B (en) | 2018-06-26 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101610668A (en) * | 2006-10-03 | 2009-12-23 | 塞米尼斯蔬菜种子公司 | Brilliant white cauliflower |
| CN103392488A (en) * | 2013-08-13 | 2013-11-20 | 镇江瑞繁农艺有限公司 | Method for raising seedlings of broccoli by cutting |
| CN103416203A (en) * | 2013-08-09 | 2013-12-04 | 镇江瑞繁农艺有限公司 | Method for axillary bud cutting seedling of common head cabbage |
| CN105532107A (en) * | 2016-01-22 | 2016-05-04 | 安徽格林亲子农庄有限公司 | Improving matrix used for improving golden cicada cultivation soil |
-
2016
- 2016-06-29 CN CN201610491840.1A patent/CN106069765B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101610668A (en) * | 2006-10-03 | 2009-12-23 | 塞米尼斯蔬菜种子公司 | Brilliant white cauliflower |
| CN103416203A (en) * | 2013-08-09 | 2013-12-04 | 镇江瑞繁农艺有限公司 | Method for axillary bud cutting seedling of common head cabbage |
| CN103392488A (en) * | 2013-08-13 | 2013-11-20 | 镇江瑞繁农艺有限公司 | Method for raising seedlings of broccoli by cutting |
| CN105532107A (en) * | 2016-01-22 | 2016-05-04 | 安徽格林亲子农庄有限公司 | Improving matrix used for improving golden cicada cultivation soil |
Non-Patent Citations (4)
| Title |
|---|
| KUNIEDA M K, ET AL.: "Bud REGENERATION OF ADVENTITIOUS ROOTS OF CAULIFLOWER TISSUE CULTURES", 《REVISTA BRASILEIRA DE BOTANICA》 * |
| PUAY-KOON CHENG, ET AL.: "HIGH-FREQUENCY DIRECT SHOOT REGENERATION AND CONTINUOUS PRODUCTION OF RAPID-CYCLING BRASSICA OLERACEA IN VITRO", 《IN VITRO CELL. DEV. BIOL.》 * |
| 李慧斌等: "观赏羽衣甘蓝扦插繁殖初步研究", 《北方园艺》 * |
| 李曙轩等: "大白菜及甘蓝的扦插繁殖方法", 《植物生理学通讯》 * |
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| CN106069765B (en) | 2018-06-26 |
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Youxing Inventor before: Hao Yumei |
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| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180523 Address after: 236300 No. 13 North Farm, Funan County Agricultural Institute, Chengta Town, Funan Town, Fuyang, Anhui Applicant after: Li Youxing Address before: 233700 Liancheng breeding farm, Liancheng Town, Guzhen County, Bengbu, Anhui, China Applicant before: Guzhen County original family farm |
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