CN106069765B - A kind of tissue method for culturing seedlings of cauliflower - Google Patents

A kind of tissue method for culturing seedlings of cauliflower Download PDF

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Publication number
CN106069765B
CN106069765B CN201610491840.1A CN201610491840A CN106069765B CN 106069765 B CN106069765 B CN 106069765B CN 201610491840 A CN201610491840 A CN 201610491840A CN 106069765 B CN106069765 B CN 106069765B
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parts
tissue
cauliflower
illumination
days
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CN201610491840.1A
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Chinese (zh)
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CN106069765A (en
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李友星
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李友星
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture; Culture media therefor

Abstract

The invention discloses a kind of tissue method for culturing seedlings of cauliflower, including tissue sampling, tissue treatment, nutritive cube plantation, seedling management and the several processes of transplanting.The present invention has the following advantages compared with prior art:It is cultivated in the present invention using the short stem of cauliflower, can effectively keep the fine quality of cauliflower;Short stem tissue is handled, can not only effectively be sterilized, moreover it is possible to effective activation histocyte;Cover composite soil after plantation, sterilization while promotes short stem-root;Stringent control illumination and intensity of illumination, avoid tissue infection, make its fast-growth;It is transplanted after bearing adventitious root, survival rate is up to more than 93.6%.

Description

A kind of tissue method for culturing seedlings of cauliflower

Technical field

The invention belongs to technical field of agricultural breeding, and in particular to a kind of tissue method for culturing seedlings of cauliflower.

Background technology

With the continuous variation of the market demand, bouquet loose type cauliflower i.e. organic cauliflower, with its light green bennet, crisp The quality of sweet tea is liked that more and more vegetable cultivation bases start that seed kind best in quality is selected to cultivate by consumers in general Organic cauliflower, but survival rate is relatively low, and easily affected by environment in cultivating process, survival rate is low after nursery, increases planting cost, Therefore it needs to study a kind of method for culturing seedlings for cauliflower, improves its survival rate.

Invention content

The purpose of the present invention is being directed to the problem of existing, a kind of tissue method for culturing seedlings of cauliflower is provided.

The present invention is achieved by the following technical solutions:A kind of tissue method for culturing seedlings of cauliflower, includes the following steps:

(1)Tissue sampling:Ripe cauliflower best in quality, that cauliflower surface layer is neat is selected, bouquet is intercepted after removing bouquet Under short stem 1-1.5cm, it is spare;

(2)Tissue treatment:The tissue of acquisition is handled with mixed liquid dipping, treatment temperature is 35-40 DEG C, and soaking time is It 1.5-2 hours, is then rinsed 3-4 times with deionized water, it is spare with sterile gauze suck dry moisture;

Wherein, mixed liquor includes the raw material of following weight percent:Carbendazim 15-20ppm, copper chloride 40-50ppm are high Potassium manganate 0.2-0.5%, indolepopionic acid potassium 20-30ppm, sodium hydroxide 5-6%, surplus is water;

(3)Nutritive cube is planted:70% seedling medium in nutritive cube is housed, processed tissue is inserted into seedling medium, Composite soil 0.5cm is covered, composite soil is formed by fine earth and recycling tealeaves dry powder blend, and mixed weight ratio is 4:1;

(4)Seedling management:After plantation in 1-5 days, 18 hours of illumination, intensity of illumination 2600-2800lx are kept;Plantation 6-15 days afterwards, keep 16 hours of illumination, intensity of illumination 2400-2500lx;After 15 days to before transplanting, illumination 14 is kept Hour, intensity of illumination 2000-2200lx;

(5)Transplanting:30 days or so after plantation, check that many adventitious roots are born in seedling root, you can transplanted.

As further improvement of these options, the grain diameter of the composite soil is 20-30 μm, water content 32- 35%。

As further improvement of these options, modifying agent is added in the seedling medium, the additive amount of modifying agent is 5-8%;

Modifying agent is made of the raw material of following parts by weight:15-20 parts of sepiolite powder, 12-18 parts of feldspar in powder, palm oil 2- 3 parts, 8-15 parts of ammonium dihydrogen phosphate, 15-20 parts of locust tree leaf powder, 5-8 parts of weathered coal, 4-6 parts of stevioside, 10-15 parts of urea, silicon Sour barium 2-4 parts, 5-8 parts of biological bacteria;

The preparation method of modifying agent is:Remaining material mixing in addition to palm oil and stevioside is uniform, suitable quantity of water is added in, Make its water content for 35-40%, under conditions of 40-50 DEG C, sealing is stacked 2-3 days, is down to room temperature after the completion, adds in palm oil And stevioside, it stirs evenly, dries, be granulated to obtain the final product.

As further improvement of these options, the biological bacteria is bacillus subtilis, and viable count is 6 × 108- 8×108cfu/g。

As further improvement of these options, the grain diameter of the modifying agent is 0.3-0.5mm, water content 8- 12%。

Appropriate modifying agent is added in seedling medium, improves the ability of matrix fertilizer and fertilizer conservation, coordinates stringent illumination control System can effectively facilitate short stem-root growth.

The present invention has the following advantages compared with prior art:It is cultivated in the present invention using the short stem of cauliflower, it can Effectively keep the fine quality of cauliflower;Short stem tissue is handled, can not only effectively be sterilized, moreover it is possible to effective activation tissue Cell;Cover composite soil after plantation, sterilization while promotes short stem-root;Stringent control illumination and intensity of illumination, avoid tissue Infection, makes its fast-growth;It is transplanted after bearing adventitious root, survival rate is up to more than 93.6%.

Specific embodiment

Embodiment 1

A kind of tissue method for culturing seedlings of cauliflower, includes the following steps:

(1)Tissue sampling:Ripe cauliflower best in quality, that cauliflower surface layer is neat is selected, bouquet is intercepted after removing bouquet Under short stem 1-1.5cm, it is spare;

(2)Tissue treatment:The tissue of acquisition is handled with mixed liquid dipping, treatment temperature is 35-40 DEG C, and soaking time is It 1.5-2 hours, is then rinsed 3-4 times with deionized water, it is spare with sterile gauze suck dry moisture;

Wherein, mixed liquor includes the raw material of following weight percent:Carbendazim 15-20ppm, copper chloride 40-50ppm are high Potassium manganate 0.2-0.5%, indolepopionic acid potassium 20-30ppm, sodium hydroxide 5-6%, surplus is water;

(3)Nutritive cube is planted:70% seedling medium in nutritive cube is housed, processed tissue is inserted into seedling medium, Composite soil 0.5cm is covered, composite soil is formed by fine earth and recycling tealeaves dry powder blend, and mixed weight ratio is 4:1;

(4)Seedling management:After plantation in 1-5 days, 18 hours of illumination, intensity of illumination 2600-2800lx are kept;Plantation 6-15 days afterwards, keep 16 hours of illumination, intensity of illumination 2400-2500lx;After 15 days to before transplanting, illumination 14 is kept Hour, intensity of illumination 2000-2200lx;

(5)Transplanting:30 days or so after plantation, check that many adventitious roots are born in seedling root, you can transplanted.

Wherein, the grain diameter of the composite soil is 20-30 μm, water content 32-35%.

Wherein, modifying agent is added in the seedling medium, the additive amount of modifying agent is 5-8%;

Modifying agent is made of the raw material of following parts by weight:15-20 parts of sepiolite powder, 12-18 parts of feldspar in powder, palm oil 2- 3 parts, 8-15 parts of ammonium dihydrogen phosphate, 15-20 parts of locust tree leaf powder, 5-8 parts of weathered coal, 4-6 parts of stevioside, 10-15 parts of urea, silicon Sour barium 2-4 parts, 5-8 parts of biological bacteria;

The preparation method of modifying agent is:Remaining material mixing in addition to palm oil and stevioside is uniform, suitable quantity of water is added in, Make its water content for 35-40%, under conditions of 40-50 DEG C, sealing is stacked 2-3 days, is down to room temperature after the completion, adds in palm oil And stevioside, it stirs evenly, dries, be granulated to obtain the final product.

Wherein, the biological bacteria is bacillus subtilis, and viable count is 6 × 108-8×108cfu/g;The modifying agent Grain diameter be 0.3-0.5mm, water content 8-12%.

Nursery is managed collectively in the present invention, survival rate of seedling is higher than 93.6%, and survival rate is high after transplanting, plantation gained flower coconut palm Dish can keep advantageous property, and cauliflower shape is loose, and bud surface is uniformly neat, and bennet is light green, edible to have crisp sweet tea mouthfeel, commodity Property is preferable.

Claims (1)

1. the tissue method for culturing seedlings of a kind of cauliflower, which is characterized in that include the following steps:
(1)Tissue sampling:Ripe cauliflower best in quality, that cauliflower surface layer is neat is selected, is intercepted under bouquet after removing bouquet Short stem 1-1.5cm, it is spare;
(2)Tissue treatment:The tissue of acquisition is handled with mixed liquid dipping, treatment temperature is 35-40 DEG C, soaking time 1.5- It 2 hours, is then rinsed 3-4 times with deionized water, it is spare with sterile gauze suck dry moisture;
Wherein, mixed liquor includes the raw material of following weight percent:Carbendazim 15-20ppm, copper chloride 40-50ppm, permanganic acid Potassium 0.2-0.5%, indolepopionic acid potassium 20-30ppm, sodium hydroxide 5-6%, surplus is water;
(3)Nutritive cube is planted:70% seedling medium in nutritive cube is housed, processed tissue is inserted into seedling medium, covering Composite soil 0.5cm, composite soil are formed by fine earth and recycling tealeaves dry powder blend, and mixed weight ratio is 4:1;
(4)Seedling management:After plantation in 1-5 days, 18 hours of illumination, intensity of illumination 2600-2800lx are kept;6- after plantation 15 days, keep 16 hours of illumination, intensity of illumination 2400-2500lx;After 15 days to before transplanting, keep illumination 14 small When, intensity of illumination 2000-2200lx;
(5)Transplanting:30 days or so after plantation, check that many adventitious roots are born in seedling root, you can transplanted;Wherein, institute The grain diameter for stating composite soil is 20-30 μm, water content 32-35%;
Wherein, modifying agent is added in the seedling medium, the additive amount of modifying agent is 5-8%;
Modifying agent is made of the raw material of following parts by weight:15-20 parts of sepiolite powder, 12-18 parts of feldspar in powder, 2-3 parts of palm oil, 8-15 parts of ammonium dihydrogen phosphate, 15-20 parts of locust tree leaf powder, 5-8 parts of weathered coal, 4-6 parts of stevioside, 10-15 parts of urea, barium silicate 2-4 parts, 5-8 parts of biological bacteria;The biological bacteria is bacillus subtilis, and viable count is 6 × 108-8×108cfu/g;
The preparation method of modifying agent is:Remaining material mixing in addition to palm oil and stevioside is uniform, suitable quantity of water is added in, makes it Water content is 35-40%, and under conditions of 40-50 DEG C, sealing is stacked 2-3 days, is down to room temperature after the completion, adds in palm oil and sweet tea Synanthrin stirs evenly, and drying is granulated to obtain the final product;
Wherein, the grain diameter of the modifying agent is 0.3-0.5mm, water content 8-12%.
CN201610491840.1A 2016-06-29 2016-06-29 A kind of tissue method for culturing seedlings of cauliflower CN106069765B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101610668A (en) * 2006-10-03 2009-12-23 塞米尼斯蔬菜种子公司 Brilliant white cauliflower
CN103392488A (en) * 2013-08-13 2013-11-20 镇江瑞繁农艺有限公司 Method for raising seedlings of broccoli by cutting
CN103416203A (en) * 2013-08-09 2013-12-04 镇江瑞繁农艺有限公司 Method for axillary bud cutting seedling of common head cabbage
CN105532107A (en) * 2016-01-22 2016-05-04 安徽格林亲子农庄有限公司 Improving matrix used for improving golden cicada cultivation soil

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101610668A (en) * 2006-10-03 2009-12-23 塞米尼斯蔬菜种子公司 Brilliant white cauliflower
CN103416203A (en) * 2013-08-09 2013-12-04 镇江瑞繁农艺有限公司 Method for axillary bud cutting seedling of common head cabbage
CN103392488A (en) * 2013-08-13 2013-11-20 镇江瑞繁农艺有限公司 Method for raising seedlings of broccoli by cutting
CN105532107A (en) * 2016-01-22 2016-05-04 安徽格林亲子农庄有限公司 Improving matrix used for improving golden cicada cultivation soil

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KUNIEDA M K, et al..Bud REGENERATION OF ADVENTITIOUS ROOTS OF CAULIFLOWER TISSUE CULTURES.《Revista Brasileira de Botanica》.1986,第9卷(第2期),第231-238页. *
PUAY-KOON CHENG, et al..HIGH-FREQUENCY DIRECT SHOOT REGENERATION AND CONTINUOUS PRODUCTION OF RAPID-CYCLING BRASSICA OLERACEA IN VITRO.《In Vitro Cell. Dev. Biol.》.2001,第37卷第592-598页. *
李慧斌等.观赏羽衣甘蓝扦插繁殖初步研究.《北方园艺》.2010,(第10期),第89-92页. *
李曙轩等.大白菜及甘蓝的扦插繁殖方法.《植物生理学通讯》.1980,(第1期),第41-44页. *

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