CN105766370A - Method for utilizing blue-green algae for planting high-quality oyster mushrooms - Google Patents

Method for utilizing blue-green algae for planting high-quality oyster mushrooms Download PDF

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Publication number
CN105766370A
CN105766370A CN201610134629.4A CN201610134629A CN105766370A CN 105766370 A CN105766370 A CN 105766370A CN 201610134629 A CN201610134629 A CN 201610134629A CN 105766370 A CN105766370 A CN 105766370A
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mushroom
oyster mushrooms
blue
blue algae
content
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沈成友
章建国
李宏国
魏兆军
胡飞
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C9/00Fertilisers containing urea or urea compounds

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a method for utilizing blue-green algae for planting high-quality oyster mushrooms.The method comprises the following specific steps that 1, blue-green algae powder and cotton seed hulls are mixed uniformly, an auxiliary material and water are added, and the mixture is bagged and manufactured into fungous material bags; 2, the fungous material bags are inoculated with oyster mushroom strains, and conventional culture is carried out; 3, oyster mushroom sporocarps are picked up.According to the method, the nutrition quality of the oyster mushrooms cultivated with the blue-green algae powder as an organic nitrogen source is superior to that of oyster mushrooms cultivated through a traditional method.By means of detection, the quantity of dry substances in the oyster mushrooms cultivated through blue-green algae is about 20% higher than that of dry substances in the oyster mushrooms produced through the traditional method, the crude fat content in the oyster mushrooms produced through the method is about 35% lower than that in the oyster mushrooms produced through the traditional method, the crude protein content is about 5% higher than that in the oyster mushrooms produced through the traditional method, the total sugar content is about 45% higher than that in the oyster mushrooms produced through the traditional method, and the contents of six kinds of essential amino acid needed by the human body are improved to the different degrees.

Description

A kind of method utilizing cyanophyceae plantation high-quality Pleurotus ostreatus
Technical field
The invention belongs to planting edible mushroom field, specifically with cotton seed hulls and blue algae powder for major ingredient, by reasonably composite, make Pleurotus ostreatus cultivation material, through inoculation, cultivate and obtain the high nutritive value mushroom carpophore that total sugar and essential amino acid content are high, crude fat content is low.
Background technology
Pleurotus ostreatus meat fertilizer is tender, delicious flavour, is one of maximum edible fungi of cultivation amount in the world.Wild Pleurotus ostreatus is the raw bacterium of a kind of wood, but through long-term domestication, present tame Pleurotus ostreatus is transferred to by " wood raw " " grass is raw ", and the culture medium of cultivating white mushroom, often based on agricultural crop straw, is aided with a small amount of wheat bran, Testa oryzae and the sucrose of trace, quick lime etc..Cyanophyceae is the aquatic property plant of a kind of low grade, and recently as the aggravation of body eutrophication, summer, the cyanophyceae in some waters often grew in burst, had had a strong impact on water quality around and ecological environment.Rich in organic matter in Cells of Blue-green Algae, balanced in nutrition, it is collected the edible fungi of cultivation high nutritive value, environmental pollution can be solved, agricultural production and income can be promoted again.
Summary of the invention
In order to reduce environmental pollution, improving water quality, improve the value of cyanophyceae simultaneously, the present invention provides a kind of method utilizing cyanophyceae plantation high-quality Pleurotus ostreatus.
A kind of operating procedure utilizing cyanophyceae to plant high-quality Pleurotus ostreatus is as follows: (1) 1:8~9 in mass ratio are by blue algae powder and cotton seed hulls mix homogeneously, and add adjuvant and water, and bacterium pocket is made in pack;(2) in bacterium pocket, access flat mushroom strain, cultivate routinely;(3) pluck mushroom carpophore, after testing, in mushroom carpophore dry total sugar content more than 45%, the content of crude protein improve 5%, crude fat content is less than 2.0%.
The concrete operation step of a kind of restriction further utilizing cyanophyceae plantation high-quality Pleurotus ostreatus is as follows:
(1) blue algae powder is made
Take the lake water of blue algae bloom growth, through dewatering, dry, smash, grind, prepare blue algae powder;
(2) bacterium pocket is made
By cotton seed hulls 88.8 grams, blue algae powder 10 grams, quick lime 1 gram, 0.1 gram of carbamide, white sugar 0.1 gram and 160 grams of mix homogeneously of tap water, wherein quick lime, carbamide and white sugar are adjuvant;Cotton seed hulls, blue algae powder, adjuvant and water are mixed thoroughly, packed, sterilizing, obtain bacterium pocket;
(3) flat mushroom strain, cultivation are accessed
Being respectively connected to flat mushroom strain at the two ends of bacterium pocket, inoculum concentration is 1~2%, cultivates 28-30 days for 25-30 DEG C, and bacterium material surface starts to grow button, carries out normal management of producing mushroom, until mushroom carpophore is ripe;Pluck mushroom carpophore.
The Advantageous Effects of the present invention is embodied in following aspect:
Pleurotus ostreatus cultivation material is the culture medium of Pleurotus ostreatus, provides whole, sufficient nutrient for mushroom growth.Nutrient can be divided into carbon source, nitrogenous source, the energy, inorganic salt and somatomedin, and wherein carbon nitrogen source is the nutrient that mushroom growth requirement is very big, and the physicochemical property of carbon nitrogen source can directly influence the Pleurotus ostreatus sorption enhanced efficiency to nutrient substance.
1. in cyanophyceae cultivating white mushroom dry, total sugar content is high
The Pleurotus ostreatus of normal growth, the content of its intracellular organic nitrogen and carbohydrate is certain ratio.In the process of cultivating white mushroom in the conventional way, the nitrogenous source of Pleurotus ostreatus is inorganic nitrogen or simple organic nitrogen such as carbamide, these low simple nitrogenous sources such as grade change into the itrogenous organic substance that time multiplexed cell is assorted, need complicated biochemical reaction, combined coefficient is low, organonitrogen compound limited source, causes that quantity keeps with it the synthesis of carbohydrate of balance also restricted;And with blue-green algae dry powder for nitrogenous source cultivating white mushroom, Medium Culture contains the organonitrogen compound that large amount of complex is senior, it can directly be absorbed and synthesize without accent by Pleurotus ostreatus cell, efficiency is greatly improved, " bottleneck " in organonitrogen compound source is released from, the content of corresponding carbohydrate raises naturally, so the Pleurotus ostreatus cultivated for nitrogenous source with cyanophyceae, in its dry, total sugar content is high, with the Pleurotus ostreatus that cyanophyceae is planted for nitrogenous source, in its dry, total sugar content is more than 45%, and the Pleurotus ostreatus planted in the conventional way, in dry, total sugar content is typically between 30%-35%.
2. in cyanophyceae cultivating white mushroom dry, crude fat content is low
Fat in organism is a kind of energy rich compound, and when internal saccharide surplus, unnecessary sugar can change into fat, and the synthesis of fat is generally present in the later stage of biological growth.As it was previously stated, the Pleurotus ostreatus organic nitrogen source of cyanophyceae cultivation is sufficient, it is possible to keeping the vigorous growth of long period, the fat of cylinder accumulation is less;The Pleurotus ostreatus of traditional method cultivation, although organic nitrogen source is limited, but internal saccharide comes from the decomposition of stalk cellulose, relatively sufficient, compared with cyanophyceae mushroom, its internal carbon-nitrogen ratio is higher, and unnecessary saccharide is directly translated into fat, so fat content is high, with the Pleurotus ostreatus that cyanophyceae is planted for nitrogenous source, in its dry, fat content is less than 2.0%, and the Pleurotus ostreatus planted in the conventional way, in dry, fat content is typically between 2.5%-3.0%.
3. in cyanophyceae cultivating white mushroom dry, essential amino acid content is high
Protein is the material base that life entity carries out various forms of vital movement.In Cells of Blue-green Algae, protein content is high, and the kind of Amino Acids in Proteins and quantity are more suitable for the dietary requirements of the mankind, using the teaching of the invention it is possible to provide all of essential amino acids of human body.
In blue-green algae dry powder, the content of crude protein is about 50%, is one natural " protein library ", and essential amino acid is the main component in cyanophyceae crude protein.Planting Pleurotus ostreatus with blue-green algae dry powder for nitrogenous source, Pleurotus ostreatus need not synthesize at the aminoacid of necessary for growth in accent, directly absorbs from substrate, and efficiency is high.Our testing result shows: in 15 seed amino acids detected, and has the content of 11 seed amino acids to exceed tradition mushroom in cyanophyceae mushroom;Have detected 7 kinds of essential amino acids, wherein 6 kinds of content in cyanophyceae mushroom are above tradition mushroom.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Mushroom cultivation kind used by the present invention is purchased from south, Hefei City, Anhui Province seven strain plants.
Embodiment 1
As follows with blue-green algae dry powder for the concrete operation step of organic nitrogen source cultivating white mushroom:
1. make blue algae powder:
The lake water of 1 ton of blue algae bloom growth, 60 DEG C of constant temperature dryings after centrifuge dehydration, grind to obtain 100 grams of cyanophyceae powder.
2. make Pleurotus ostreatus cultivation material:
Weigh 100 grams of blue algae powders and 890 grams of cotton seed hullss, mix thoroughly;Weigh 10 grams of quick lime, 1 gram of carbamide and 1 gram of white sugar, being dissolved in 1.6 liters of tap waters and obtain aqueous solution, obtain wet feed with this aqueous solution and the blue algae powder mixed thoroughly before and cotton seed hulls mix homogeneously, gained wet feed subpackage enters the bacterium pocket of 3 50 × 20cm, two ends tying, 121 DEG C of high pressure steam sterilizations 1 hour.
3. access mushroom cultivation kind and cultivation:
Being respectively connected to 10 grams of mushroom cultivation kinds at the two ends of bacterium pocket, be placed in 28 DEG C of mold incubators and cultivate, relative humidity keeps 60%.Cultivating 28 days, hypha of Pleurotus ostreatus is long even long real in planting material, and the nearly mouth place of bacterium pocket starts Pleurotus ostreatus button occur, increases water spray number of times, improves intensity of illumination, until growing the mushroom carpophore of maturation.
4. Pleurotus ostreatus is plucked
Being further cultured for 7 days, the base portion at mushroom carpophore starts villous white hypha occur, plucks mushroom carpophore.
Table 1. macronutrient content (%) and relative different
Table 2. essential amino acid content (milligrams per gram) and relative different
Table 3. non-essential amino acid content (milligrams per gram) and relative different
After mushroom carpophore is plucked, the pulp choosing upper end carries out Analysis of Nutritive Composition, analyzes result in Table 1, table 2 and table 3: wherein the mass percentage content of total sugar is 46.22%, and the content of crude protein improves 5.54%, and crude fat content is less than 2.0%.The amino acid content of needed by human nearly all has growth.
Embodiment 2
1. make blue algae powder:
Taking the lake water of 50 tons of blue algae bloom growths, centrifuge dehydration, spread out even drying, dry to constant weight for 60 DEG C, 5 kilograms of cyanophyceae powder pulverized to obtain by pulverizer.
2. make Pleurotus ostreatus cultivation material:
Weigh 5 kilograms of blue algae powders and 45 kilograms of cotton seed hullss, mix thoroughly;Weigh 500 grams of quick lime, 50 grams of carbamide and 50 grams of white sugars, it is dissolved in 80 kilograms of tap waters and obtains aqueous solution, wet feed is obtained with this aqueous solution and the blue algae powder mixed thoroughly before and cotton seed hulls mix homogeneously, gained wet feed subpackage enters the bacterium pocket of 150 50 × 20cm, two ends tying, atmospheric steam sterilizing 10 hours.
3. access mushroom cultivation kind and cultivation:
Being respectively connected to 10 grams of mushroom cultivation kinds at the two ends of bacterium pocket, be placed in 25 DEG C of mushroom sheds lucifuge and cultivate, relative humidity keeps 60%.Cultivating 32 days, hypha of Pleurotus ostreatus is long even long real in planting material, and the nearly mouth place of bacterium pocket starts Pleurotus ostreatus button occur, increases water spray number of times, improves intensity of illumination, until growing the mushroom carpophore of maturation.
4. Pleurotus ostreatus is plucked
Being further cultured for 7 days, the base portion at mushroom carpophore starts villous white hypha occur, plucks mushroom carpophore.
Table 4. macronutrient content (%) and relative different
Table 5. essential amino acid content (milligrams per gram) and relative different
Table 6. non-essential amino acid content (milligrams per gram) and relative different
After mushroom carpophore is plucked, the pulp choosing upper end carries out Analysis of Nutritive Composition, analyzes result in Table 4, table 5 and table 6: wherein the mass percentage content of total sugar is 48.89%, and the content of crude protein improves 5.7%, and crude fat content is less than 2.0%.The amino acid content of needed by human nearly all has growth.

Claims (2)

1. the method utilizing cyanophyceae plantation high-quality Pleurotus ostreatus, it is characterised in that operating procedure is as follows: (1) 1:8~9 in mass ratio are by blue algae powder and cotton seed hulls mix homogeneously, and add adjuvant and water, and bacterium pocket is made in pack;(2) in bacterium pocket, access flat mushroom strain, cultivate routinely;(3) pluck mushroom carpophore, after testing, in mushroom carpophore dry total sugar content more than 45%, the content of crude protein improve 5%, crude fat content is less than 2.0%.
2. a kind of method utilizing cyanophyceae plantation high-quality Pleurotus ostreatus according to claim 1, it is characterised in that concrete operation step is as follows:
(1) blue algae powder is made
Take the lake water of blue algae bloom growth, through dewatering, dry, smash, grind, prepare blue algae powder;
(2) bacterium pocket is made
By cotton seed hulls 88.8 grams, blue algae powder 10 grams, quick lime 1 gram, 0.1 gram of carbamide, white sugar 0.1 gram and 160 grams of mix homogeneously of tap water, wherein quick lime, carbamide and white sugar are adjuvant;Cotton seed hulls, blue algae powder, adjuvant and water are mixed thoroughly, packed, sterilizing, obtain bacterium pocket;
(3) flat mushroom strain, cultivation are accessed
Being respectively connected to flat mushroom strain at the two ends of bacterium pocket, inoculum concentration is 1~2%, cultivates 28-30 days for 25-30 DEG C, and bacterium material surface starts to grow button, carries out normal management of producing mushroom, until mushroom carpophore is ripe;Pluck mushroom carpophore.
CN201610134629.4A 2016-03-09 2016-03-09 Method for utilizing blue-green algae for planting high-quality oyster mushrooms Pending CN105766370A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107189951A (en) * 2017-06-15 2017-09-22 柳城新天地生态农业发展有限公司 The method for improving amino acid content in elegant precious mushroom
CN111543252A (en) * 2020-05-08 2020-08-18 福建环海生物科技股份有限公司 Seaweed gel extraction residue post-treatment process, fungus culture medium and fungus culture method

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Publication number Priority date Publication date Assignee Title
CN102783359A (en) * 2012-08-10 2012-11-21 安徽天都灵芝制品公司 Method for culturing oyster mushroom by adopting raw materials
CN102826905A (en) * 2012-08-25 2012-12-19 刘海荣 Oyster mushroom cultivation mixture
CN103155801A (en) * 2011-12-16 2013-06-19 詹文圆 Method for producing oyster mushroom culture medium by using blue-green algae and sawdust to serve as main raw materials
CN103155799A (en) * 2011-12-16 2013-06-19 詹文圆 Method for producing high-quality needle mushroom culture medium by using blue-green algae, corncobs and broad leaf tree sawdust to serve as main raw materials
CN104429617A (en) * 2014-12-19 2015-03-25 苏州市经纬农产品有限公司 Oyster mushroom cultivation method
CN104756761A (en) * 2015-04-02 2015-07-08 乌鲁木齐高鑫博渊生物科技有限公司 High-yield cultivation method of edible fungus
CN105175108A (en) * 2015-08-20 2015-12-23 青岛华盛绿能农业科技有限公司 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103155801A (en) * 2011-12-16 2013-06-19 詹文圆 Method for producing oyster mushroom culture medium by using blue-green algae and sawdust to serve as main raw materials
CN103155799A (en) * 2011-12-16 2013-06-19 詹文圆 Method for producing high-quality needle mushroom culture medium by using blue-green algae, corncobs and broad leaf tree sawdust to serve as main raw materials
CN102783359A (en) * 2012-08-10 2012-11-21 安徽天都灵芝制品公司 Method for culturing oyster mushroom by adopting raw materials
CN102826905A (en) * 2012-08-25 2012-12-19 刘海荣 Oyster mushroom cultivation mixture
CN104429617A (en) * 2014-12-19 2015-03-25 苏州市经纬农产品有限公司 Oyster mushroom cultivation method
CN104756761A (en) * 2015-04-02 2015-07-08 乌鲁木齐高鑫博渊生物科技有限公司 High-yield cultivation method of edible fungus
CN105175108A (en) * 2015-08-20 2015-12-23 青岛华盛绿能农业科技有限公司 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107189951A (en) * 2017-06-15 2017-09-22 柳城新天地生态农业发展有限公司 The method for improving amino acid content in elegant precious mushroom
CN111543252A (en) * 2020-05-08 2020-08-18 福建环海生物科技股份有限公司 Seaweed gel extraction residue post-treatment process, fungus culture medium and fungus culture method

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