CN106561269A - Cultivation material for enhancing pleurotus geesteranus yield and amino acid content and cultivation method - Google Patents
Cultivation material for enhancing pleurotus geesteranus yield and amino acid content and cultivation method Download PDFInfo
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- CN106561269A CN106561269A CN201610980459.1A CN201610980459A CN106561269A CN 106561269 A CN106561269 A CN 106561269A CN 201610980459 A CN201610980459 A CN 201610980459A CN 106561269 A CN106561269 A CN 106561269A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention relates to a cultivation material for enhancing a pleurotus geesteranus yield and an amino acid content. The cultivation material that is formed by mixing of water and the following raw materials based on a weight proportion; and the raw materials include: 15-20 parts of mushroom dreg, 22-28 parts of wood bits, 22 to 28 parts of cottonseed hull, 8-12 parts of chicken manure ash, 18 to 22 parts of bran, 0.5 to 1.5 parts of calcium carbonate and 0. 5-1.5 parts of lime. The mass percentage of water in the cultivation material is 62 to 68% and the pH value is 7.5 to 8.5. With the cultivation material and cultivation method, a defect that the yield and amino acid content are low and the delicious degree is low because of the poor-quality cultivation material for pleurotus geesteranus production and bad cultivation method can be overcome. Moreover, the pleurotus geesteranus yield can increase and the amino acid content can be improved simultaneously; and the cost is low.
Description
Technical field
The present invention relates to a kind of planting material for improving Pleurotus geesteranus yield and amino acid content and elegant using planting material cultivation
The cultural method of precious mushroom, applies in Pleurotus geesteranus cultivation.
Background technology
Pleurotus geesteranus (Pleurotus geesteranus) belong to Eumycota, Basidiomycetess, gill fungi on taxonomy Pleurotus geesteranus
Mesh, Pleurotaceae pleurotus.Title derives from Taiwan, and different from common Pleurotus sajor-caju, it is because which is less, handle has 5-6 centimetre,
Lid diameter<3 centimetres.3.65-3.88% containing protein in Pleurotus geesteranus, crude fat 1.13-1.18%, reducing sugar 0.87-1.80%,
Sugar 23.94-34.87%, lignin 2.64%, cellulose 12.85%, pectin 0.14%, also containing cellulose, minerals
Deng.It can be seen that, Pleurotus geesteranus are a kind of high protein, low-fat nutraceutical, and it is tasty, with unique local flavor, beautiful its name
Say " monosodium glutamate mushroom ".But due to consumption demand it is vigorous, the yield and quality of existing Pleurotus geesteranus (amino acid content number it is fresh with edible fungi
U.S. correlation of attributes) it is difficult to meet the market demand, still need to further lift which.Although existing Zheng is proposed to beautiful et al.
Adopt collect core stalk dual-purpose Semen arachidis hypogaeae maturation harvest when aerial partss straw dries pulverizing after with 10-20wt% substitute wood
The method of bits cultivation Pleurotus geesteranus, to obtain the Pleurotus geesteranus sporophore of high yield simultaneously while can be by the aminoacid of Pleurotus geesteranus sporophore
Content improves 13.06% and (refers to《A kind of method for improving Pleurotus geesteranus content of glutamic acid》, Application No. is 201410200170.4).
But the yield of the Pleurotus geesteranus sporophore produced by the method is lifted less, and amino acid content lifts limited extent, to Pleurotus geesteranus
Mouthfeel difficulty has big change.Therefore provide it is a kind of can be while greatly improving Pleurotus geesteranus yield and amino acid content and with low cost carrying
The planting material of high Pleurotus geesteranus yield and amino acid content and using planting material cultivation Pleurotus geesteranus cultural method oneself become when business
Urgently.
The content of the invention
In order to overcome the caused yield of the not good institute of planting material and cultural method and amino acid content of existing Pleurotus geesteranus production
The shortcoming of relatively low (delicious degree is not good), the present invention provide a kind of planting material and its cultivation for improving Pleurotus geesteranus yield and amino acid content
Culture method, having the advantages that can be while improves Pleurotus geesteranus yield and amino acid content and with low cost.
Technical scheme is as follows:
A kind of planting material for improving Pleurotus geesteranus yield and amino acid content, by the raw material mixing of water and following weight proportion
Into the raw material includes:Bacteria residue 15-20 parts, wood flour 22-28 parts, cotton seed hullss 22-28 parts, chicken manure generating ash 8-12 parts, wheat bran
18-22 parts, Calcium Carbonate 0.5-1.5 part, Calx 0.5-1.5 parts, in the planting material, the weight/mass percentage composition of water is 62-68%,
PH value is 7.5-8.5.
It is different from Pleurotus geesteranus planting material of the tradition with wood flour, cotton seed hullss, bagasse, corn cob, wheat bran as major ingredient, the application
Pleurotus geesteranus planting material major ingredient in additionally use chicken manure generate electricity ash and bacteria residue.Chicken manure generating ash is drawn materials in spontaneous power plant using chicken
Waste material after excrement generating, which is excellent phosphate fertilizer and potash fertilizer, and in collocation, the secondary bacteria residue for utilizing, improves traditional cultivation material formula
Major ingredient nutritional labeling.And compare, resource reutilization is not only realized using the chicken manure ash that generates electricity, and is dropped
The low bacteria containing amount of planting material, reduces the probability of varied bacteria growing.The recycling of waste bacterial slag, can not only be to the remnants of bacteria residue
Nutrition carries out secondary utilization, and loose porous beneficial to mycelial growth, green, environmental protection and low-carbon (LC).Bacteria residue generates electricity grey with chicken manure
And the raw material components of remaining planting material being collocated with each other and interacting, significantly improve Pleurotus geesteranus yield and aminoacid contains
Amount, due to the amino acid content how much delicious degree positive correlation with edible fungi, therefore, the quality of Pleurotus geesteranus is improved.Should
Planting material while the yield and amino acid content of Pleurotus geesteranus is effectively improved, also so that chicken manure generate electricity after and edible fungus culturing
Waste material afterwards is fully utilized, and solves the problems, such as and has expanded the recycling of the grey and discarded planting material of chicken manure after generating electricity, enter one
Step reduces Pleurotus geesteranus production cost, reduces the wasting of resources, alleviates environmental pollution, and economic results in society significantly, are that production is high
The Pleurotus geesteranus for producing high-quality open a Tiao Xin roads.
The raw material includes:17 parts of bacteria residue, 26 parts of wood flour, 25 parts of cotton seed hullss, 10 parts of chicken manure generating ash, 20 parts of wheat bran, carbon
1 part of sour calcium, 1 part of Calx, pH value is 8.
The Pleurotus geesteranus planting material is preferred scheme, realizes the further lifting of Pleurotus geesteranus yield and amino acid content.
In the planting material, the weight/mass percentage composition of water is 65%.
The ratio of water to material is optimal, both can guarantee that Pleurotus geesteranus planting material for mycelial growth and provides sufficient moisture, and had been avoided that water again
Divide the environment beneficial to fungus growth for excessively causing.
The bacteria residue is formed by planting material crushing discarded after seafood mushroom is cultivated.
The planting material remnants nutritional labelings of the bacteria residue can be secondary use, and the seafood mycelium for wherein remaining is alternatively
The growth of Pleurotus geesteranus provides special nutriment, with chicken manure generate electricity collocation together with ash, effect can effectively improve Pleurotus geesteranus yield and
Amino acid content.The planting material of the seafood mushroom can adopt existing various formula, for example can be by Caulis et Folium Oryzae, wheat straw, cotton seed
Shell, corn cob, crop material, Testa oryzae, wheat bran, Semen sojae atricolor powder, Semen Maydis powder, Cotton Seed powder etc. are constituted.
A kind of cultural method that Pleurotus geesteranus are cultivated using above-mentioned planting material, including the following steps for carrying out successively:
1) material preparation step:First in stirring container one add stirring equal remaining raw material in addition to Calcium Carbonate and Calx
It is even;Then, after Calcium Carbonate and Calx being dissolved in a small amount of water in stirring container two, stir in adding stirring container one;
Finally continue in container one, add water to stir so that mixed planting material reaches described water content;Afterwards, will plant
Training material pack carries out autoclaving;
2) inoculation step:After step 1) sterilizing after cultivation material bag temperature be down to≤27 DEG C when, by Pleurotus geesteranus strain be inoculated with
Into the planting material;
3) mycelia culture step:Treat step 2) cultivation material bag that has been inoculated with moves into the mycelia culture room that temperature is 22-26 DEG C
Culture 85-88 days;
4) management of producing mushroom and harvesting step:Step 3 is covered with after mycelium) after cultivation material bag, the cultivation material bag is moved into
In mushroom house after sterilization, mushroom house humiture is controlled, timely collecting is carried out during the non-parachute-opening of mushroom lid after fruiting.
The Pleurotus geesteranus cultural method effectively reduces production cost, and convenient to operation.
The step 3) in mycelia culture temperature be 24 DEG C, cultivated days be 86 days.
The growth of the mycelia culture temperature and cultivated days optimum oyster mushroom pieces, can make full use of planting material.
The step 4) in mushroom house temperature be 15-20 DEG C, the humidity of mushroom house is 89-97%.
The mushroom house humiture can not only reduce the infection probability of miscellaneous bacteria, and good permeability causes oyster mushroom pieces to grow
It is vigorous, beneficial to Pleurotus geesteranus fruiting.
The step 4) in mushroom house sterilization concretely comprise the following steps:18- is added with every cubic metre of space 8-12g potassium permanganate
The amount of 22ml formaldehyde carries out fumigation to mushroom house, and door and window ventilation is opened after 24 hours.
The fumigation is quick effectively, without dead angle.
Compared with prior art, the present patent application has advantages below:
1) it is substantially improved using the yield of Pleurotus geesteranus after the Pleurotus geesteranus planting material, equal > 15%;Except glutamic acid, Guang ammonia
Acid, L-Tyrosine, outside alanine, remaining 13 kinds aminoacid (aspartic acid, threonine, serine, glycine, Rhizoma et radix valerianae propylhomoserin,
Methionine, isoleucine, leucine, Phenylalanine, lysine, histidine, arginine, proline) content have >=
10% greatly improves, wherein, the amplification of aspartic acid, methionine, lysine, arginine and proline content is maximum,
>=20% can be about reached averagely;
2) the Pleurotus geesteranus planting material employs chicken manure and generates electricity ash, not only abundant raw material source, save labor, and be conducive to
The negative effect completely using chemical nitrogen is solved, more directly using the bacteria containing amount that planting material is reduced for chicken manure, is expanded
The use range of chicken manure generating ash, provides new culture medium for Pleurotus geesteranus cultivation, is that circular agriculture explores one effectively
Technological approaches, open an effective development Pleurotus geesteranus artificial culture and the practical road for expanding the scale of production;
3) the Pleurotus geesteranus planting material employs the useless bacteria residue of cultivation seafood mushroom, not only realizes the recycling of useless bacteria residue, and energy
Arrange in pairs or groups, the special nutriment of Pleurotus geesteranus growth offer is provided with chicken manure generating ash, contained with the yield and aminoacid that improve Pleurotus geesteranus
Amount;
4) cultural method using planting material cultivation Pleurotus geesteranus is easy to operate, low cost.
Specific embodiment
Below technical scheme is described in detail.
Embodiment 1
A kind of planting material for improving Pleurotus geesteranus yield and amino acid content of the present invention, by water and following weight proportion
Raw material mix, the raw material includes:17 parts of bacteria residue, 26 parts of wood flour, 25 parts of cotton seed hullss, chicken manure generating grey 10 parts, wheat bran
20 parts, 1 part of Calcium Carbonate, 1 part of Calx, in the planting material weight/mass percentage composition of water be 65%, pH value is 8.The bacteria residue by
After cultivation seafood mushroom, discarded planting material is crushed and is formed.
A kind of cultural method that Pleurotus geesteranus are cultivated using above-mentioned planting material, including the following steps for carrying out successively:
1) material preparation step:First in stirring container one add stirring equal remaining raw material in addition to Calcium Carbonate and Calx
It is even;Then, after Calcium Carbonate and Calx being dissolved in a small amount of water in stirring container two, stir in adding stirring container one;
Finally continue in container one, add water to stir so that mixed planting material reaches described water content;Afterwards, will plant
Training material pack carries out autoclaving;
2) inoculation step:After step 1) sterilizing after cultivation material bag temperature be down to≤27 DEG C when, by Pleurotus geesteranus strain be inoculated with
Into the planting material;
3) mycelia culture step:Treat step 2) cultivation material bag that has been inoculated with moves into the mycelia culture room culture that temperature is 24 DEG C
86 days;
4) management of producing mushroom and harvesting step:Step 3 is covered with after mycelium) after cultivation material bag, the cultivation material bag is moved into
It is 16 DEG C control mushroom house temperature in mushroom house after sterilization, and humidity is 92%, after fruiting carries out timely collecting during the non-parachute-opening of mushroom lid.
The step 4) in mushroom house sterilization concretely comprise the following steps:20ml first is added with every cubic metre of space 10g potassium permanganate
The amount of aldehyde carries out fumigation to mushroom house, and door and window ventilation is opened after 24 hours.
Embodiment 2
Which with the difference of embodiment 1 is:The raw material includes:15 parts of bacteria residue, 28 parts of wood flour, 22 parts of cotton seed hullss, chicken manure
Generate electricity 12 parts of ash, 18 parts of wheat bran, 1.5 parts of Calcium Carbonate, 0.5 part of Calx, and in the planting material, the weight/mass percentage composition of water is 68%,
PH value is 7.5.The bacteria residue is crushed by planting material discarded after Xinbao mushroom culturing and is formed.
It is described Pleurotus geesteranus are cultivated using above-mentioned planting material cultural method the step of 1) in planting material pack be per packed
Material about 230g, autoclave time about 2.5h;Step 3) in mycelia culture temperature be 22 DEG C, cultivated days be 88 days;The step
It is rapid 4) in mushroom house temperature be 19 DEG C, the humidity of mushroom house is 97%;What the mushroom house was sterilized concretely comprises the following steps:It is empty with per cubic metre
Between 8g potassium permanganate add the amount of 22ml formaldehyde that fumigation is carried out to mushroom house, after 24 hours open door and window ventilation.
Embodiment 3
Which with the difference of embodiment 1 is:The raw material includes:20 parts of bacteria residue, 22 parts of wood flour, 28 parts of cotton seed hullss, chicken manure
Generate electricity 8 parts of ash, 22 parts of wheat bran, 0.5 part of Calcium Carbonate, 1.5 parts of Calx, and in the planting material, the weight/mass percentage composition of water is 62%,
PH value is 8.5.The bacteria residue is crushed by planting material discarded after cultivating flammulina velutipes and is formed.
It is described Pleurotus geesteranus are cultivated using above-mentioned planting material cultural method the step of 3) in mycelia culture temperature be 26 DEG C,
Cultivated days are 85 days;The step 4) in mushroom house temperature be 20 DEG C, the humidity of mushroom house is 89%;The tool of the mushroom house sterilization
Body step is:Add with every cubic metre of space 12g potassium permanganate the amount of 18ml formaldehyde that fumigation is carried out to mushroom house, beat after 24 hours
Door window ventilation.
Test data
(1) different impacts of the planting material to the various amino acid contents of Pleurotus geesteranus
Per 100g 17 kinds of aminoacid of Pleurotus geesteranus in the Pleurotus geesteranus and each embodiment of the planting material cultivation of measure prior art
Content.
Various amino acid content (the units of Pleurotus geesteranus of 1 different planting material cultivations of table:g/100g)
Testing result shows, with the Pleurotus geesteranus of planting material cultivation of the invention compared with the show cultivated using prior art planting material
Precious mushroom, in addition to glutamic acid, cystine, L-Tyrosine, alanine, remaining 13 kinds amino acid content is greatly improved, wherein,
The amplification of aspartic acid, methionine, lysine, arginine and proline content is maximum, more than 20%.Wherein, it is real
The total amino acidss content for applying example 1 improves 10.14%, is optimal case.
(2) the different impacts of planting material and cultural method to Pleurotus geesteranus yield
Every batch Pleurotus geesteranus yield of the planting material and each embodiment planting material of prior art is determined, wherein, per batch it is
35 bag cultivating material, per bag cultivating material dry weight about 230g, every kind of planting material carries out 3 batches altogether and repeats to test.
The Pleurotus geesteranus yield of 2 different planting material cultivations of table
Shown by 3 repetition result of the tests of upper table:Shadow of the planting material and cultural method of the application to Pleurotus geesteranus yield
Ring notable, the yield of Pleurotus geesteranus significantly can be provided, the wherein average growth rate of embodiment 1, up to 22.43%, is optimal case.
The planting material and its cultural method of raising Pleurotus geesteranus yield of the present invention and amino acid content is not only only
Be confined to above-described embodiment, every any improvement or replacement according to the principle of the invention, all should protection scope of the present invention it
It is interior.
Claims (10)
1. a kind of planting material for improving Pleurotus geesteranus yield and amino acid content, it is characterised in that:The planting material is by water and following
The raw material of weight proportion is mixed, and the raw material includes:Bacteria residue 15-20 parts, wood flour 22-28 parts, cotton seed hullss 22-28 parts, chicken
Excrement generating ash 8-12 parts, wheat bran 18-22 parts, Calcium Carbonate 0.5-1.5 part, Calx 0.5-1.5 parts, the quality of water in the planting material
Percentage composition is 62-68%, and pH value is 7.5-8.5.
2. the planting material for improving Pleurotus geesteranus yield and amino acid content according to claim 1, it is characterised in that:The original
Material includes:17 parts of bacteria residue, 26 parts of wood flour, 25 parts of cotton seed hullss, 10 parts of chicken manure generating ash, 20 parts of wheat bran, 1 part of Calcium Carbonate, Calx 1
Part, pH value is 8.
3. the planting material for improving Pleurotus geesteranus yield and amino acid content according to claim 2, it is characterised in that:The cultivation
In training material, the weight/mass percentage composition of water is 65%.
4. the planting material for improving Pleurotus geesteranus yield and amino acid content according to claim 1, it is characterised in that:The bacterium
Slag is formed by planting material crushing discarded after seafood mushroom is cultivated.
5. the planting material for improving Pleurotus geesteranus yield and amino acid content according to claim 2, it is characterised in that:The bacterium
Slag is formed by planting material crushing discarded after seafood mushroom is cultivated.
6. the planting material for improving Pleurotus geesteranus yield and amino acid content according to claim 3, it is characterised in that:The bacterium
Slag is formed by planting material crushing discarded after seafood mushroom is cultivated.
7. a kind of usage right requires that the planting material described in any one of 1-6 cultivates the cultural method of Pleurotus geesteranus, it is characterised in that:Bag
Include the following steps for carrying out successively:
1) material preparation step:First remaining raw material in addition to Calcium Carbonate and Calx is added in stirring container one and stirred;Connect
After Calcium Carbonate and Calx being dissolved in a small amount of water in stirring container two, stir in adding stirring container one;Finally
Continue in container one, add water to stir so that mixed planting material reaches described water content;Afterwards, by planting material
Pack carries out autoclaving;
2) inoculation step:After step 1) sterilizing after cultivation material bag temperature be down to≤27 DEG C when, Pleurotus geesteranus strain is seeded to into institute
State in planting material;
3) mycelia culture step:Treat step 2) cultivation material bag that has been inoculated with moves into the mycelia culture room that temperature is 22-26 DEG C and cultivates
85-88 days;
4) management of producing mushroom and harvesting step:Step 3 is covered with after mycelium) after cultivation material bag, the cultivation material bag is moved into and is sterilized
In mushroom house afterwards, mushroom house humiture is controlled, timely collecting is carried out during the non-parachute-opening of mushroom lid after fruiting.
8. it is according to claim 7 cultivation Pleurotus geesteranus cultural method, it is characterised in that:The step 3) in mycelia culture
Temperature is 24 DEG C, and cultivated days are 86 days.
9. it is according to claim 7 cultivation Pleurotus geesteranus cultural method, it is characterised in that:The step 4) in mushroom house temperature
Spend for 15-27 DEG C, the humidity of mushroom house is 89-97%.
10. it is according to claim 7 cultivation Pleurotus geesteranus cultural method, it is characterised in that:The step 4) in mushroom house
That what is sterilized concretely comprises the following steps:Add the amount of 18-22ml formaldehyde that stifling disappearing is carried out to mushroom house with every cubic metre of space 8-12g potassium permanganate
Poison, opens door and window ventilation after 24 hours.
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CN107189951A (en) * | 2017-06-15 | 2017-09-22 | 柳城新天地生态农业发展有限公司 | The method for improving amino acid content in elegant precious mushroom |
CN107188647A (en) * | 2017-06-22 | 2017-09-22 | 柳城新天地生态农业发展有限公司 | The cultural method of coprinus comatus |
CN108651158A (en) * | 2018-05-22 | 2018-10-16 | 江山惠企科技服务有限公司 | Elegant precious mushroom and seafood mushroom cultivation method |
CN108812060A (en) * | 2018-07-09 | 2018-11-16 | 镇江市菇满园生态农业有限公司 | A kind of method of off-season cultivation Taiwan elegant precious mushroom |
CN109479619A (en) * | 2019-01-22 | 2019-03-19 | 缙云县珍稀食用菌专业合作社 | A method of improving 4-Aminobutanoicacid content in elegant precious mushroom fructification |
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CN110521493A (en) * | 2019-01-29 | 2019-12-03 | 金华市挺美科技有限公司 | A kind of elegant precious mushroom culture substrate and preparation method thereof |
CN113519348A (en) * | 2021-07-20 | 2021-10-22 | 百色学院 | Method and device for cultivating pleurotus geesteranus by using culture medium containing sunflower discs |
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CN107188647A (en) * | 2017-06-22 | 2017-09-22 | 柳城新天地生态农业发展有限公司 | The cultural method of coprinus comatus |
CN108651158A (en) * | 2018-05-22 | 2018-10-16 | 江山惠企科技服务有限公司 | Elegant precious mushroom and seafood mushroom cultivation method |
CN108812060A (en) * | 2018-07-09 | 2018-11-16 | 镇江市菇满园生态农业有限公司 | A kind of method of off-season cultivation Taiwan elegant precious mushroom |
CN109479619A (en) * | 2019-01-22 | 2019-03-19 | 缙云县珍稀食用菌专业合作社 | A method of improving 4-Aminobutanoicacid content in elegant precious mushroom fructification |
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CN113519348A (en) * | 2021-07-20 | 2021-10-22 | 百色学院 | Method and device for cultivating pleurotus geesteranus by using culture medium containing sunflower discs |
CN113519348B (en) * | 2021-07-20 | 2024-03-29 | 百色学院 | Method and device for cultivating pleurotus geesteranus by using sunflower-disc-containing culture medium |
CN114402908A (en) * | 2022-01-20 | 2022-04-29 | 福建农林大学 | Efficient pleurotus geesteranus fruiting cultivation method by hanging bag marking |
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