CN105493851A - Bletilla striata seedling domestication method - Google Patents
Bletilla striata seedling domestication method Download PDFInfo
- Publication number
- CN105493851A CN105493851A CN201510942244.6A CN201510942244A CN105493851A CN 105493851 A CN105493851 A CN 105493851A CN 201510942244 A CN201510942244 A CN 201510942244A CN 105493851 A CN105493851 A CN 105493851A
- Authority
- CN
- China
- Prior art keywords
- seedling
- bletilla striata
- nursery pond
- water content
- seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001313857 Bletilla striata Species 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 82
- 239000000022 bacteriostatic agent Substances 0.000 claims abstract description 28
- 239000000758 substrate Substances 0.000 claims abstract description 26
- 239000003337 fertilizer Substances 0.000 claims abstract description 13
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 8
- 238000009736 wetting Methods 0.000 claims abstract description 8
- 239000011159 matrix material Substances 0.000 claims description 22
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 17
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical group C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 17
- 239000006013 carbendazim Substances 0.000 claims description 17
- 241000195940 Bryophyta Species 0.000 claims description 14
- 239000003415 peat Substances 0.000 claims description 14
- 229920006327 polystyrene foam Polymers 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 229910052902 vermiculite Inorganic materials 0.000 claims description 14
- 235000019354 vermiculite Nutrition 0.000 claims description 14
- 239000010455 vermiculite Substances 0.000 claims description 14
- 229920006395 saturated elastomer Polymers 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 10
- 244000037666 field crops Species 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 8
- 238000002386 leaching Methods 0.000 claims description 7
- 238000002054 transplantation Methods 0.000 claims description 7
- 230000004083 survival effect Effects 0.000 abstract description 7
- 230000002262 irrigation Effects 0.000 abstract 3
- 238000003973 irrigation Methods 0.000 abstract 3
- 244000052769 pathogen Species 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 238000007605 air drying Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 description 2
- 241001313855 Bletilla Species 0.000 description 2
- 229920002581 Glucomannan Polymers 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229930003949 flavanone Natural products 0.000 description 2
- 150000002208 flavanones Chemical class 0.000 description 2
- 235000011981 flavanones Nutrition 0.000 description 2
- 229940046240 glucomannan Drugs 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 241000195628 Chlorophyta Species 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 208000000616 Hemoptysis Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010061577 Ulcer haemorrhage Diseases 0.000 description 1
- 230000003816 axenic effect Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 201000009267 bronchiectasis Diseases 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses a Bletilla striata seedling domestication method. The method comprises following steps: a seedling culture substrate is prepared; Bletilla striata seedlings are disinfected, Bletilla striata tissue culture seedlings are subjected to root soaking treatment in a bacteriostatic agent after exercising; then seedling culture management is performed: excessive water in a nursery pond is drained after the Bletilla striata seedlings in the nursery pond survive, so that the water content of the substrate is reduced gradually and naturally; irrigation is performed after the water content of the substrate is lower than 30%; irrigation is repeated after the water content of the substrate is lower than 30%; the seedlings are transplanted to a field: after alternation of drying and wetting and repeated irrigation for 45 days, single weight of Bletilla striata fruits is higher than 4 g, and the Bletilla striata seedlings are transplanted to the field for planting. Seedling domestication steps and method are optimized, scientific seedling culture management is adopted, so that a root rot phenomenon is avoided for the Bletilla striata seedlings after exercising, pathogens are inhibited by the bacteriostatic agent, reasonable fertilizer efficiency is guaranteed by multi-compound fertilizer, the Bletilla striata tissue culture seedlings can be domesticated rapidly, the transplanting survival rate of the Bletilla striata seedlings is increased remarkably, and the seedling culture cost is reduced.
Description
Technical field
The present invention relates to field of plant growing technology, be specifically related to a kind of bletilla striata seedling domesticating method.
Background technology
The rare crude drugs bletilla striata (BletillaStriata (Thunb.) Reichb.f.), for the bletilla striata belongs to herbaceos perennial, is national key protected wild plants, has high economic worth.The bletilla striata is used as medicine with dry tuber, has astringing to arrest bleeding, effect of detumescence and promoting granulation, is widely used in treatment pulmonary tuberculosis and the disease such as bronchiectasis hemoptysis, ulcer bleeding.The bletilla striata is except for except medical aspect, and bletilla striata stem tuber is rich in Bletilla glucomannan, and Bletilla glucomannan can replace west yellow work rubber powder, gummi arabicum pulveratum as emulsifier, suspending agent for fields such as food, beauty treatment and chemical industry, and tool has been widely used.
In recent years; along with the expansion of its purposes; market demand increases year by year; price rises year by year; cause going to wreck property of wild resource to be excavated, the plant being now put into the Precious, Rare, Endangered of country is protected, but also is recorded in international trade pact (CITES) annex two; be subject to the International Protection, forbid wild resource trade.Carrying out artificial planting is the effective way solving bletilla striata imbalance between supply and demand, protection bletilla striata wild resource.At present with bletilla striata seeds axenic germination to obtain a large amount of bletilla striata seedling, but plantlet in vitro be transplanted to land for growing field crops after survival rate not high, the industrialized development of the serious restriction bletilla striata.
Summary of the invention
The object of this invention is to provide and a kind ofly can improve the method that bletilla striata plantlet in vitro is transplanted to survival rate behind land for growing field crops; Method of the present invention can significantly improve the transplanting survival rate of bletilla striata seedling, reduces seedling cost, shortens growing-seedling period, makes up the deficiencies in the prior art.
For reaching above-mentioned purpose, providing a kind of bletilla striata seedling domesticating method in one embodiment of the present of invention, comprising:
(1) seedling medium: add bacteriostatic agent after the peat composed of rotten mosses, vermiculite and wormcast being mixed in proportion, and adjust water content and obtain seedling medium; Matrix is loaded in polystyrene foam seedling-cultivating tray stand-by;
(2) bletilla striata seedling sterilization: after carrying out the process of leaching root after bletilla striata training tissue culture seedling in bacteriostatic agent, drain away the water; Then the bletilla striata seedling after sterilization is planted in seedling-cultivating tray, then put into nursery pond;
(3) seedling management: after the bletilla striata seedling in nursery pond survives, excessive moisture in discharge nursery pond, makes the water content of matrix naturally reduce gradually; Pouring process is carried out after water content of substrate is lower than 30%; The detailed process of described pouring process allows seedling-cultivating tray be immersed in water for pouring water in nursery pond, and in nursery pond, add bacteriostatic agent and multi-element compound fertilizer; After water content of substrate is saturated, again put the moisture of dry nursery pond, allow matrix become dry gradually; After water content of substrate is lower than 30%, repeat pouring process;
(4) field-transplanting: when repeatedly carrying out alternation of wetting and drying, repeating pouring process after 45 days, the substance of bletilla striata fruit, at more than 4g, is planted in bletilla striata transplantation of seedlings to land for growing field crops.
In optimal enforcement example of the present invention, in seedling medium, the weight ratio of the peat composed of rotten mosses, vermiculite and wormcast is 6:3:1.
In optimal enforcement example of the present invention, the water content of described seedling medium is 25% ~ 35%.
In optimal enforcement example of the present invention, polystyrene foam nursery is 162 holes, and appearance and size is 66.5cm × 34cm × 5.5cm.
In optimal enforcement example of the present invention, bacteriostatic agent is carbendazim; In nursery pond, the final concentration of bacteriostatic agent is 0.05% ~ 0.15%.
In optimal enforcement example of the present invention, in nursery pond, the final concentration of multi-element compound fertilizer is 150ppm ~ 200ppm.
In sum, the present invention has the following advantages:
The present invention tames and dociles seedling step and method by optimizing, have employed the seedling management of science, ensure that the bletilla striata seedling after hardening there will not be bad root phenomenon, and suppressing pathogene by bacteriostatic agent, multi-element compound fertilizer ensures reasonable fertilizer efficiency, can tame bletilla striata plantlet in vitro fast, significantly improve the transplanting survival rate of bletilla striata seedling, reduce seedling cost, shorten growing-seedling period, make up the deficiencies in the prior art.
Embodiment
Build nursery pond: nursery pond can be built in standard plastic booth, if there is no plastic tunnel, then need to take other to shelter from heat or light and the measure of taking shelter from rain.
Nursery pond can surround the Fang Chi of length 1500cm, wide 140cm, dark 20cm with brick, length can adjust according to site condition, as long as ensure that the nursery pond water surface can occupy by seedling-cultivating tray, in order to avoid the outburst of nursery pond green algae.Enclosing well carries out smooth to soil, and for convenience of draining, when leveling land, nursery pond keeps certain gradient, presets discharge outlet at low side, is then doing at the bottom of pond and pool side with film.Nursery pond is built up and to be discharged water in nursery pond afterwards, and the depth of water remains on 5cm ~ 8cm.
Embodiment 1
A kind of bletilla striata seedling domesticating method, comprising:
(1) seedling medium: add bacteriostatic agent after the peat composed of rotten mosses, vermiculite and wormcast being mixed in proportion, in seedling medium, the weight ratio of the peat composed of rotten mosses, vermiculite and wormcast is 6:3:1, and obtains seedling medium about adjusting water content to 30%; Matrix is loaded in polystyrene foam seedling-cultivating tray stand-by; Polystyrene foam nursery is 162 holes, and appearance and size is 66.5cm × 34cm × 5.5cm.
(2) bletilla striata seedling sterilization: drain away the water carrying out the process of leaching root after bletilla striata training tissue culture seedling in bacteriostatic agent carbendazim solution after 10 minutes; Carbendazim dilutes 1000 times, and concentration is 0.1%; Then the bletilla striata seedling after sterilization is planted in seedling-cultivating tray, then put into nursery pond;
(3) seedling management: after the bletilla striata seedling in nursery pond survives, excessive moisture in discharge nursery pond, makes the water content of matrix naturally reduce gradually; Pouring process is carried out after water content of substrate reduces by 28%.The detailed process of pouring process allows seedling-cultivating tray be immersed in water for pouring water in nursery pond, and in nursery pond, to add bacteriostatic agent carbendazim to final concentration be 0.1%, and adding multi-element compound fertilizer to its content is 150ppm.After water content of substrate is saturated, again put the moisture of dry nursery pond, allow matrix become dry gradually; When water content of substrate is low to moderate after 30%, repeat pouring process several times; Allow water in the saturated rear discharge nursery pond of water supply in media, make its natural air drying, in the pouring that discharges water after being low to moderate 30%.
(4) field-transplanting: when repeatedly carrying out alternation of wetting and drying, repeating pouring process after 45 days, the substance of bletilla striata fruit, when 5g, is planted in bletilla striata transplantation of seedlings to land for growing field crops.
Embodiment 2
A kind of bletilla striata seedling domesticating method, comprising:
(1) seedling medium: add bacteriostatic agent after the peat composed of rotten mosses, vermiculite and wormcast being mixed in proportion, in seedling medium, the weight ratio of the peat composed of rotten mosses, vermiculite and wormcast is 5:2:1, and obtains seedling medium about adjusting water content to 28%; Matrix is loaded in polystyrene foam seedling-cultivating tray stand-by; Polystyrene foam nursery is 162 holes, and appearance and size is 66.5cm × 34cm × 5.5cm.
(2) bletilla striata seedling sterilization: drain away the water carrying out the process of leaching root after bletilla striata training tissue culture seedling in bacteriostatic agent carbendazim solution after 9 minutes; Carbendazim dilutes 1000 times, and concentration is 0.1%; Then the bletilla striata seedling after sterilization is planted in seedling-cultivating tray, then put into nursery pond;
(3) seedling management: after the bletilla striata seedling in nursery pond survives, excessive moisture in discharge nursery pond, makes the water content of matrix naturally reduce gradually; Pouring process is carried out after water content of substrate reduces by 30%.The detailed process of pouring process allows seedling-cultivating tray be immersed in water for pouring water in nursery pond, and in nursery pond, to add bacteriostatic agent carbendazim to final concentration be 0.12%, and adding multi-element compound fertilizer to its content is 200ppm.After water content of substrate is saturated, again put the moisture of dry nursery pond, allow matrix become dry gradually; When water content of substrate is low to moderate after 28%, repeat pouring process several times; Allow water in the saturated rear discharge nursery pond of water supply in media, make its natural air drying, in the pouring that discharges water after being low to moderate 29%.
(4) field-transplanting: when repeatedly carrying out alternation of wetting and drying, repeating pouring process after 50 days, the substance of bletilla striata fruit, when 4.5g, is planted in bletilla striata transplantation of seedlings to land for growing field crops.
Embodiment 3
A kind of bletilla striata seedling domesticating method, comprising:
(1) seedling medium: add bacteriostatic agent after the peat composed of rotten mosses, vermiculite and wormcast being mixed in proportion, in seedling medium, the weight ratio of the peat composed of rotten mosses, vermiculite and wormcast is 5:2:1, and obtains seedling medium about adjusting water content to 28%; Matrix is loaded in polystyrene foam seedling-cultivating tray stand-by; Polystyrene foam nursery is 162 holes, and appearance and size is 66.5cm × 34cm × 5.5cm.
(2) bletilla striata seedling sterilization: drain away the water carrying out the process of leaching root after bletilla striata training tissue culture seedling in bacteriostatic agent carbendazim solution after 15 minutes; Carbendazim dilutes 1000 times, and concentration is 0.1%; Then the bletilla striata seedling after sterilization is planted in seedling-cultivating tray, then put into nursery pond;
(3) seedling management: after the bletilla striata seedling in nursery pond survives, excessive moisture in discharge nursery pond, makes the water content of matrix naturally reduce gradually; Pouring process is carried out after water content of substrate reduces by 30%.The detailed process of pouring process allows seedling-cultivating tray be immersed in water for pouring water in nursery pond, and in nursery pond, to add bacteriostatic agent carbendazim to final concentration be 0.08%, and adding multi-element compound fertilizer to its content is 180ppm.After water content of substrate is saturated, again put the moisture of dry nursery pond, allow matrix become dry gradually; When water content of substrate is low to moderate after 30%, repeat pouring process several times; Allow water in the saturated rear discharge nursery pond of water supply in media, make its natural air drying, in the pouring that discharges water after being low to moderate 25%.
(4) field-transplanting: when repeatedly carrying out alternation of wetting and drying, repeating pouring process after 42 days, the substance of bletilla striata fruit, when 4.8g, is planted in bletilla striata transplantation of seedlings to land for growing field crops.
Embodiment 4
A kind of bletilla striata seedling domesticating method, comprising:
(1) seedling medium: add bacteriostatic agent after the peat composed of rotten mosses, vermiculite and wormcast being mixed in proportion, in seedling medium, the weight ratio of the peat composed of rotten mosses, vermiculite and wormcast is 6:3:1, and obtains seedling medium about adjusting water content to 30%; Matrix is loaded in polystyrene foam seedling-cultivating tray stand-by; Polystyrene foam nursery is 162 holes, and appearance and size is 66.5cm × 34cm × 5.5cm.
(2) bletilla striata seedling sterilization: drain away the water carrying out the process of leaching root after bletilla striata training tissue culture seedling in bacteriostatic agent carbendazim solution after 10 minutes; Carbendazim dilutes 1000 times, and concentration is 0.1%; Then the bletilla striata seedling after sterilization is planted in seedling-cultivating tray, then put into nursery pond;
(3) seedling management: after the bletilla striata seedling in nursery pond survives, excessive moisture in discharge nursery pond, makes the water content of matrix naturally reduce gradually; Pouring process is carried out after water content of substrate reduces by 28%.The detailed process of pouring process allows seedling-cultivating tray be immersed in water for pouring water in nursery pond, and in nursery pond, to add bacteriostatic agent carbendazim to final concentration be 0.1%, and adding multi-element compound fertilizer to its content is 150ppm, and adding flavanones to final concentration is 0.05%.After adding flavanones, improve bletilla striata seedling resistance against diseases, improve survival rate, and its survival rate improves about 10% compared with embodiment 1, has significant difference.After water content of substrate is saturated, again put the moisture of dry nursery pond, allow matrix become dry gradually; When water content of substrate is low to moderate after 30%, repeat pouring process several times; Allow water in the saturated rear discharge nursery pond of water supply in media, make its natural air drying, in the pouring that discharges water after being low to moderate 30%.
(4) field-transplanting: when repeatedly carrying out alternation of wetting and drying, repeating pouring process after 45 days, the substance of bletilla striata fruit, when 5g, is planted in bletilla striata transplantation of seedlings to land for growing field crops.
Embodiment 5
A kind of bletilla striata seedling domesticating method, comprising:
(1) seedling medium: add bacteriostatic agent after the peat composed of rotten mosses, vermiculite and wormcast being mixed in proportion, in seedling medium, the weight ratio of the peat composed of rotten mosses, vermiculite and wormcast is 6:3:1, and obtains seedling medium about adjusting water content to 30%; Matrix is loaded in polystyrene foam seedling-cultivating tray stand-by; Polystyrene foam nursery is 162 holes, and appearance and size is 66.5cm × 34cm × 5.5cm.
(2) bletilla striata seedling sterilization: drain away the water carrying out the process of leaching root after bletilla striata training tissue culture seedling in bacteriostatic agent carbendazim solution after 10 minutes; Carbendazim dilutes 1000 times, and concentration is 0.1%; Then the bletilla striata seedling after sterilization is planted in seedling-cultivating tray, then put into nursery pond;
(3) seedling management: after the bletilla striata seedling in nursery pond survives, excessive moisture in discharge nursery pond, makes the water content of matrix naturally reduce gradually; Pouring process is carried out after water content of substrate reduces by 28%.The detailed process of pouring process allows seedling-cultivating tray be immersed in water for pouring water in nursery pond, and in nursery pond, to add bacteriostatic agent carbendazim to final concentration be 0.1%, and adding multi-element compound fertilizer to its content is 150ppm.After water content of substrate is saturated, again put the moisture of dry nursery pond, allow matrix become dry gradually, after nursery pond is poured water, use the wavelength of 380nm ~ 420nm to carry out irradiation bletilla striata seedling, in the process of nursery pond draining matrix natural air drying, use the wavelength of 720nm ~ 780nm to irradiate.The present invention is by selecting different wavelength range to carry out illumination when different substrates water content, compare embodiment 1 and shorten 4 ~ 5 days seedling raise periods, greatly reduce the time that bletilla striata seedling is cultivated in nursery pond, bletilla striata fruit growth rate is improved, field-transplanting can be carried out in advance.When water content of substrate is low to moderate after 30%, repeat pouring process several times; Allow water in the saturated rear discharge nursery pond of water supply in media, make its natural air drying, in the pouring that discharges water after being low to moderate 30%.
(4) field-transplanting: when repeatedly carrying out alternation of wetting and drying, repeating pouring process after 45 days, the substance of bletilla striata fruit, when 5g, is planted in bletilla striata transplantation of seedlings to land for growing field crops.
Claims (7)
1. a bletilla striata seedling domesticating method, comprising:
(1) seedling medium: add bacteriostatic agent after the peat composed of rotten mosses, vermiculite and wormcast being mixed in proportion, and adjust water content and obtain seedling medium; Matrix is loaded in polystyrene foam seedling-cultivating tray stand-by;
(2) bletilla striata seedling sterilization: after carrying out the process of leaching root after bletilla striata training tissue culture seedling in bacteriostatic agent, drain away the water; Then the bletilla striata seedling after sterilization is planted in seedling-cultivating tray, then put into nursery pond;
(3) seedling management: after the bletilla striata seedling in nursery pond survives, excessive moisture in discharge nursery pond, makes the water content of matrix naturally reduce gradually; Pouring process is carried out after water content of substrate is lower than 30%; The detailed process of described pouring process allows seedling-cultivating tray be immersed in water for pouring water in nursery pond, and in nursery pond, add bacteriostatic agent and multi-element compound fertilizer; After water content of substrate is saturated, again put the moisture of dry nursery pond, allow matrix become dry gradually; After water content of substrate is lower than 30%, repeat pouring process;
(4) field-transplanting: when repeatedly carrying out alternation of wetting and drying, repeating pouring process after 45 days, the substance of bletilla striata fruit, at more than 4g, is planted in bletilla striata transplantation of seedlings to land for growing field crops.
2. the method for claim 1, is characterized in that: in described seedling medium, the weight ratio of the peat composed of rotten mosses, vermiculite and wormcast is 6:3:1.
3. the method for claim 1, is characterized in that: the water content of described seedling medium is 25% ~ 35%.
4. the method for claim 1, is characterized in that: described polystyrene foam nursery is 162 holes, and appearance and size is 66.5cm × 34cm × 5.5cm.
5. the method for claim 1, is characterized in that: described bacteriostatic agent is carbendazim.
6. the method for claim 1, is characterized in that: in described nursery pond, the final concentration of bacteriostatic agent is 0.05% ~ 0.15%.
7. the method for claim 1, is characterized in that: in described nursery pond, the final concentration of multi-element compound fertilizer is 150ppm ~ 200ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510942244.6A CN105493851B (en) | 2015-12-16 | 2015-12-16 | A kind of bletilla striata seedling acclimation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510942244.6A CN105493851B (en) | 2015-12-16 | 2015-12-16 | A kind of bletilla striata seedling acclimation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105493851A true CN105493851A (en) | 2016-04-20 |
| CN105493851B CN105493851B (en) | 2019-10-01 |
Family
ID=55702472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510942244.6A Active CN105493851B (en) | 2015-12-16 | 2015-12-16 | A kind of bletilla striata seedling acclimation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105493851B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211676A (en) * | 2017-05-26 | 2017-09-29 | 黄山峰源生物科技有限公司 | The acclimation method of bletilla tissue culture seedlings |
| CN108901720A (en) * | 2018-06-23 | 2018-11-30 | 云南省农业科学院农业环境资源研究所 | Bletilla tissue culture seedlings waveform seedling bed hardening off method |
| CN109349077A (en) * | 2018-09-26 | 2019-02-19 | 河南云帮农业科技有限公司 | A kind of bletilla striata tissue-culture container seedling domestication transplantation method |
| CN112088730A (en) * | 2020-08-18 | 2020-12-18 | 云南吉成园林科技股份有限公司 | Hemerocallis fulva tissue culture seedling domestication method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011019A (en) * | 2007-02-01 | 2007-08-08 | 张宜虎 | Seedling water cultivating method by using crop water-cultivating inducing liquid |
| CN101699985A (en) * | 2009-11-17 | 2010-05-05 | 贵州省烟草科学研究所 | Method for controlling water of seedbed of cured tobacco seedlings by floating cultivation |
| CN102550383A (en) * | 2012-03-26 | 2012-07-11 | 江苏省农业科学院 | Water-film seedling method of vegetables |
| CN102986419A (en) * | 2012-11-23 | 2013-03-27 | 南通市瑞华生物工程有限公司 | Organic seedling raising substrate |
| CN103026957A (en) * | 2013-01-10 | 2013-04-10 | 宿迁市土壤肥料技术指导站 | Tomato plug seedling matrix and preparation method thereof |
| JP2013150591A (en) * | 2011-12-26 | 2013-08-08 | Yamari Seisakusho:Kk | Method for cultivating plant, and medium, cultivation vessel and cultivation apparatus used for the same |
| CN103392574A (en) * | 2013-08-02 | 2013-11-20 | 中国烟草总公司重庆市公司烟草科学研究所 | Method for growing seedlings by using intermittent flow type nutrient liquid film |
-
2015
- 2015-12-16 CN CN201510942244.6A patent/CN105493851B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011019A (en) * | 2007-02-01 | 2007-08-08 | 张宜虎 | Seedling water cultivating method by using crop water-cultivating inducing liquid |
| CN101699985A (en) * | 2009-11-17 | 2010-05-05 | 贵州省烟草科学研究所 | Method for controlling water of seedbed of cured tobacco seedlings by floating cultivation |
| JP2013150591A (en) * | 2011-12-26 | 2013-08-08 | Yamari Seisakusho:Kk | Method for cultivating plant, and medium, cultivation vessel and cultivation apparatus used for the same |
| CN102550383A (en) * | 2012-03-26 | 2012-07-11 | 江苏省农业科学院 | Water-film seedling method of vegetables |
| CN102986419A (en) * | 2012-11-23 | 2013-03-27 | 南通市瑞华生物工程有限公司 | Organic seedling raising substrate |
| CN103026957A (en) * | 2013-01-10 | 2013-04-10 | 宿迁市土壤肥料技术指导站 | Tomato plug seedling matrix and preparation method thereof |
| CN103392574A (en) * | 2013-08-02 | 2013-11-20 | 中国烟草总公司重庆市公司烟草科学研究所 | Method for growing seedlings by using intermittent flow type nutrient liquid film |
Non-Patent Citations (1)
| Title |
|---|
| 张洁等: "贵州道地药材白芨工厂化育苗技术", 《种子》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211676A (en) * | 2017-05-26 | 2017-09-29 | 黄山峰源生物科技有限公司 | The acclimation method of bletilla tissue culture seedlings |
| CN108901720A (en) * | 2018-06-23 | 2018-11-30 | 云南省农业科学院农业环境资源研究所 | Bletilla tissue culture seedlings waveform seedling bed hardening off method |
| CN109349077A (en) * | 2018-09-26 | 2019-02-19 | 河南云帮农业科技有限公司 | A kind of bletilla striata tissue-culture container seedling domestication transplantation method |
| CN112088730A (en) * | 2020-08-18 | 2020-12-18 | 云南吉成园林科技股份有限公司 | Hemerocallis fulva tissue culture seedling domestication method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105493851B (en) | 2019-10-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100393195C (en) | Method for artificial dormancy-relieving and early focing culturing | |
| CN102356746A (en) | Technology for cultivating organic fruit and vegetable matrix | |
| CN102598917B (en) | Cultivation method for breaking through woodbine seed dormancy and improving survival rate of seedlings | |
| CN103444305B (en) | A kind of method of the cultivation impelling miracle fruit seed numerous soon | |
| CN100571503C (en) | A kind of elaeagnus mollis asexual reproduction method | |
| CN105724170B (en) | Hibiscus hamabo container seedling culture method | |
| CN105493851A (en) | Bletilla striata seedling domestication method | |
| CN107969273A (en) | A kind of mangrove plant xylocarpus granatum seedling breeding method | |
| CN1582630A (en) | Cultivation of eucommia | |
| CN104982217A (en) | Paris polyphylla seed propagating method | |
| CN103636374B (en) | The light filling seedling-cultivating method of lijiang spruce container seedling and application | |
| CN103283340A (en) | Method for promoting germination of cercidiphyllum japonicum seeds and increasing germination rate of cercidiphyllum japonicum seeds | |
| CN106856711A (en) | A kind of method for promoting apocarya seed quick-speed germination | |
| CN100407897C (en) | Roxburgh anoectochilus cultivating device and technology | |
| CN105210827B (en) | Phase in early spring Yew cuttage method for culturing seedlings | |
| CN104642000B (en) | Manglietia lucida seed germinating and seedling method | |
| CN104285748A (en) | Method for improving survival rate of pachira macrocarpa seed seedling | |
| CN104718903A (en) | Apple branch cutting seedling method | |
| CN103238446B (en) | Seedling growing method for Blumea balsamifera seeds | |
| CN106856945A (en) | A kind of Idesia polycarpa cottage method | |
| CN103039240B (en) | Seeding and seedling raising method for waterbirch in Three Gorges Reservoir region | |
| CN106613264B (en) | Bletilla striata tissue culture seedling transplanting domestication technology | |
| CN108770669A (en) | A kind of breeding method of the excellent container seedling of apocarya | |
| CN109006147A (en) | A kind of implantation methods of dragon spruce | |
| CN102577698A (en) | Treatment method of white flower loropetalum chinense seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191031 Address after: 410000 Hunan province Changsha Furong district Mapoling Province Academy of Agricultural Sciences Patentee after: HUNAN INSTITUTE OF AGRO-ENVIRONMENT AND ECOLOGY Address before: The first floor of the science and technology exchange center of the two road 892 Provincial Agricultural Academy of Furong district, Changsha, Hunan Patentee before: HUNAN INSTITUTE OF WATERMELON & MUSKMELON |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210107 Address after: 416000 Zhenxi office, Jishou City, Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province Patentee after: Xiangxi Autonomous Prefecture Hongsheng eco agricultural tourism development Co.,Ltd. Address before: 410000, Mapoling Academy of Agricultural Sciences, Furong district, Changsha City, Hunan Province Patentee before: HUNAN INSTITUTE OF AGRO-ENVIRONMENT AND ECOLOGY |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231103 Address after: 416000 Innovation and Entrepreneurship Incubator Area C, 3rd Floor, Wulingshan Innovation and Entrepreneurship Demonstration Park Building, No. 48 Industrial Avenue, Xiangxi High tech Zone, Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province 115 Patentee after: Hunan Jiade Taikang Biotechnology Co.,Ltd. Address before: 416000 Zhenxi office, Jishou City, Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province Patentee before: Xiangxi Autonomous Prefecture Hongsheng eco agricultural tourism development Co.,Ltd. |
|
| TR01 | Transfer of patent right |