CN105378074A - 在无血清悬浮细胞培养系统中生产重组慢病毒载体的可扩大的制造方法 - Google Patents

在无血清悬浮细胞培养系统中生产重组慢病毒载体的可扩大的制造方法 Download PDF

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Publication number
CN105378074A
CN105378074A CN201480016236.2A CN201480016236A CN105378074A CN 105378074 A CN105378074 A CN 105378074A CN 201480016236 A CN201480016236 A CN 201480016236A CN 105378074 A CN105378074 A CN 105378074A
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methods according
virus vector
vector
factor
virus
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曲光
约翰·弗雷泽·莱特
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Childrens Hospital of Philadelphia CHOP
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Manufacturing & Machinery (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201480016236.2A 2013-03-15 2014-03-17 在无血清悬浮细胞培养系统中生产重组慢病毒载体的可扩大的制造方法 Pending CN105378074A (zh)

Priority Applications (1)

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CN202410062902.1A CN118256453A (zh) 2013-03-15 2014-03-17 在无血清悬浮细胞培养系统中生产重组慢病毒载体的可扩大的制造方法

Applications Claiming Priority (3)

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US201361787818P 2013-03-15 2013-03-15
US61/787,818 2013-03-15
PCT/US2014/030370 WO2014145578A1 (en) 2013-03-15 2014-03-17 Scalable manufacturing process to produce recombinant lentiviral vectors in serum-free suspension cell culture system

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CN202410062902.1A Division CN118256453A (zh) 2013-03-15 2014-03-17 在无血清悬浮细胞培养系统中生产重组慢病毒载体的可扩大的制造方法

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CN202410062902.1A Pending CN118256453A (zh) 2013-03-15 2014-03-17 在无血清悬浮细胞培养系统中生产重组慢病毒载体的可扩大的制造方法

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US (2) US20140323556A1 (enExample)
EP (1) EP2970920B1 (enExample)
JP (1) JP6480910B6 (enExample)
KR (1) KR102200278B1 (enExample)
CN (2) CN105378074A (enExample)
AU (1) AU2014232879B2 (enExample)
BR (1) BR112015022292A2 (enExample)
CA (1) CA2904366C (enExample)
DK (1) DK2970920T3 (enExample)
ES (1) ES2681434T3 (enExample)
IL (1) IL241058B (enExample)
MX (1) MX364690B (enExample)
PT (1) PT2970920T (enExample)
RU (1) RU2709678C2 (enExample)
WO (1) WO2014145578A1 (enExample)
ZA (1) ZA201506478B (enExample)

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CN106497882A (zh) * 2016-10-29 2017-03-15 复旦大学 同时过表达R‑spondin1和Noggin的细胞株及其构建方法和应用
WO2018161858A1 (en) * 2017-03-06 2018-09-13 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
CN109251939A (zh) * 2017-07-14 2019-01-22 孙搏 Il-17g-197a慢病毒表达载体、其构建方法及慢病毒的包装测定方法
CN109563496A (zh) * 2016-03-30 2019-04-02 星火治疗有限公司 用于重组蛋白和/或病毒载体生产的细胞系
US10260050B2 (en) 2017-03-06 2019-04-16 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
CN110054678A (zh) * 2019-05-16 2019-07-26 中国医学科学院血液病医院（血液学研究所） 一种膜结合型mFLT3LG蛋白及其应用
CN110938654A (zh) * 2019-12-11 2020-03-31 和元生物技术（上海）股份有限公司 一种细胞转染试剂及其应用
CN113122501A (zh) * 2019-12-30 2021-07-16 重庆精准生物技术有限公司 适合大规模临床级病毒载体制备的培养基及其应用
CN113980916A (zh) * 2021-09-27 2022-01-28 湖南丰晖生物科技有限公司 一种慢病毒的纯化方法
WO2022143623A1 (zh) * 2020-12-29 2022-07-07 江苏金斯瑞蓬勃生物科技有限公司 高分散性的hek293t细胞株及其筛选方法
CN115698305A (zh) * 2020-06-11 2023-02-03 2赛文缇生物公司 用于制造病毒载体的方法
WO2023061409A1 (zh) * 2021-10-12 2023-04-20 江苏金斯瑞蓬勃生物科技有限公司 适应无血清悬浮培养的hek293细胞株及其应用
CN116240241A (zh) * 2022-12-30 2023-06-09 上海本导基因技术有限公司 重组慢病毒载体的纯化制备方法

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US11596652B2 (en) 2015-02-18 2023-03-07 Enlivex Therapeutics R&D Ltd Early apoptotic cells for use in treating sepsis
US11304976B2 (en) 2015-02-18 2022-04-19 Enlivex Therapeutics Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11497767B2 (en) 2015-02-18 2022-11-15 Enlivex Therapeutics R&D Ltd Combination immune therapy and cytokine control therapy for cancer treatment
TWI547782B (zh) * 2015-03-16 2016-09-01 智易科技股份有限公司 可擴充式製程系統與實現該系統的方法
IL287500B2 (en) 2015-04-21 2023-12-01 Enlivex Therapeutics Rdo Ltd Therapeutic pooled blood apoptotic cell preparations and uses thereof
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CN107043784A (zh) * 2016-02-05 2017-08-15 上海吉凯基因化学技术有限公司 一种慢病毒载体的制备方法
CA3014885A1 (en) 2016-02-18 2017-08-24 Enlivex Therapeutics Ltd. Combination immune therapy and cytokine control therapy for cancer treatment
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CN110317832B (zh) * 2018-03-28 2022-07-05 西比曼生物科技（香港）有限公司 Gmp级规模化制备重组慢病毒载体的纯化制剂的方法
CN111763238B (zh) * 2019-04-02 2022-07-29 普莱柯生物工程股份有限公司 一种蛋白抗原的纯化方法、制备的蛋白抗原及其应用
US12565645B2 (en) * 2019-04-11 2026-03-03 Regenxbio Inc. Methods of size exclusion chromatography for the characterization of recombinant adeno-associated virus compositions
US20220282281A1 (en) * 2019-08-05 2022-09-08 Polyplus Transfection Compositions for transfecting a nucleic acid molecule into a cell comprising triazole compounds grafted to a cationic polymer, and their applications
KR20220047635A (ko) 2019-08-23 2022-04-18 론자 워커스빌 아이엔씨. 렌티바이러스 벡터의 생산을 위한 방법 및 작제물
CN110894494B (zh) * 2019-11-22 2022-09-27 广西梧州制药（集团）股份有限公司 一种大规模高密度悬浮培养293细胞高产腺病毒的方法
EP4196596A4 (en) * 2020-08-13 2025-01-01 Tmunity Therapeutics Inc. METHOD FOR PRODUCING A CLINICAL QUALITY LENTIVIRAL VECTOR
CN112746076B (zh) * 2020-12-28 2023-05-12 中吉智药(南京)生物技术有限公司 一种密码子优化的col7a1基因及慢病毒和应用
TW202309294A (zh) * 2021-04-27 2023-03-01 瑞士商諾華公司 病毒載體生產系統
EP4373951A1 (en) * 2021-07-19 2024-05-29 2seventy bio, Inc. Vector manufacturing processes
CN114990077A (zh) * 2022-08-01 2022-09-02 深圳市先康达生命科学有限公司 一种慢病毒包装与纯化方法
IN202321021121A (enExample) * 2023-03-24 2024-04-19
KR20250172638A (ko) * 2023-04-12 2025-12-09 씨티바 바이오프로세스 알＆디 에이비 크로마토그래피 시스템, 그의 용도, 및 외피보유 또는 멤브레인성 생물학적 입자를 분리하는 방법

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Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109563496A (zh) * 2016-03-30 2019-04-02 星火治疗有限公司 用于重组蛋白和/或病毒载体生产的细胞系
CN109563496B (zh) * 2016-03-30 2023-08-29 星火治疗有限公司 用于重组蛋白和/或病毒载体生产的细胞系
CN106497882A (zh) * 2016-10-29 2017-03-15 复旦大学 同时过表达R‑spondin1和Noggin的细胞株及其构建方法和应用
US11319529B2 (en) 2017-03-06 2022-05-03 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
WO2018161858A1 (en) * 2017-03-06 2018-09-13 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
US10260050B2 (en) 2017-03-06 2019-04-16 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
US12297461B2 (en) 2017-03-06 2025-05-13 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
CN109251939A (zh) * 2017-07-14 2019-01-22 孙搏 Il-17g-197a慢病毒表达载体、其构建方法及慢病毒的包装测定方法
CN110054678B (zh) * 2019-05-16 2023-04-18 中国医学科学院血液病医院（血液学研究所） 一种膜结合型mFLT3LG蛋白及其应用
CN110054678A (zh) * 2019-05-16 2019-07-26 中国医学科学院血液病医院（血液学研究所） 一种膜结合型mFLT3LG蛋白及其应用
CN110938654A (zh) * 2019-12-11 2020-03-31 和元生物技术（上海）股份有限公司 一种细胞转染试剂及其应用
CN113122501A (zh) * 2019-12-30 2021-07-16 重庆精准生物技术有限公司 适合大规模临床级病毒载体制备的培养基及其应用
CN113122501B (zh) * 2019-12-30 2023-06-16 重庆精准生物技术有限公司 适合大规模临床级病毒载体制备的培养基及其应用
CN115698305A (zh) * 2020-06-11 2023-02-03 2赛文缇生物公司 用于制造病毒载体的方法
WO2022143623A1 (zh) * 2020-12-29 2022-07-07 江苏金斯瑞蓬勃生物科技有限公司 高分散性的hek293t细胞株及其筛选方法
CN113980916A (zh) * 2021-09-27 2022-01-28 湖南丰晖生物科技有限公司 一种慢病毒的纯化方法
WO2023061409A1 (zh) * 2021-10-12 2023-04-20 江苏金斯瑞蓬勃生物科技有限公司 适应无血清悬浮培养的hek293细胞株及其应用
CN116240241A (zh) * 2022-12-30 2023-06-09 上海本导基因技术有限公司 重组慢病毒载体的纯化制备方法

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JP2016512700A (ja) 2016-05-09
MX364690B (es) 2019-05-06
IL241058B (en) 2020-05-31
CA2904366A1 (en) 2014-09-18
MX2015012450A (es) 2016-04-25
JP6480910B6 (ja) 2019-04-03
RU2015144057A (ru) 2017-04-27
DK2970920T3 (en) 2018-08-06
PT2970920T (pt) 2018-07-30
AU2014232879A1 (en) 2015-09-24
IL241058A0 (en) 2015-11-30
JP6480910B2 (ja) 2019-03-13
US20170368201A1 (en) 2017-12-28
US20140323556A1 (en) 2014-10-30
KR102200278B1 (ko) 2021-01-07
RU2709678C2 (ru) 2019-12-19
ES2681434T3 (es) 2018-09-13
HK1219978A1 (en) 2017-04-21
AU2014232879B2 (en) 2020-03-05
CA2904366C (en) 2023-07-04
ZA201506478B (en) 2021-09-29
KR20160024841A (ko) 2016-03-07
CN118256453A (zh) 2024-06-28
BR112015022292A2 (pt) 2017-07-18
EP2970920A4 (en) 2016-09-14
US10994028B2 (en) 2021-05-04
EP2970920B1 (en) 2018-04-25
EP2970920A1 (en) 2016-01-20
WO2014145578A1 (en) 2014-09-18

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Application publication date: 20160302