CN105325252A - Cuttage propagation method for Dalbergia hupeana Hance - Google Patents

Cuttage propagation method for Dalbergia hupeana Hance Download PDF

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Publication number
CN105325252A
CN105325252A CN201510815833.8A CN201510815833A CN105325252A CN 105325252 A CN105325252 A CN 105325252A CN 201510815833 A CN201510815833 A CN 201510815833A CN 105325252 A CN105325252 A CN 105325252A
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cuttage
cutting
soil
humidity
module
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CN201510815833.8A
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Chinese (zh)
Inventor
蒋泽平
马骧
蒋小庚
邱国金
史云光
张永忠
王圳
朱朝琴
王玉刚
卞亚文
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Jiangsu Forestry Academy
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Jiangsu Forestry Academy
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to a cuttage propagation method for Dalbergia hupeana Hance, which comprises the main steps of medium preparation, nursery bed pretreatment, cutting wood collection, cutting wood treatment, cuttage and comprehensive management, wherein a cuttage medium is formed by mixing yellow soil, humus soil, peat soil and coir according to a volume ratio of 3 to 3 to 2 to 2 and disinfected with 2% formalin before cuttage, unsprouted cutting woods with diameters of 0.5-1.5cm on the upper parts of an annual mother plant are collected from July to August, cut, disinfected with 0.15% thiophanate methyl solution, soaked with 100mg/L ABT1 solution and directly inserted into a medium container, and comprehensive management of humidity, light radiation and temperature is finally performed. By adopting the method for cutting Dalbergia hupeana Hance, the cuttage survival rate is more than or equal to 90%, the germination percentage is high, the roots are developed, the seedling establishment period is short and economical benefit is good.

Description

A kind of yellow wingceltis cuttage and seedling culture method
Technical field
The present invention relates to yellow wingceltis breeding field, particularly relate to a kind of yellow wingceltis cuttage and seedling culture method.
Background technology
Yellow wingceltis, formal name used at school: DalbergiahupeanaHance, Papilionaceae, Dalbergia magaphanerophytes.Bark lead, winglike compound leaf, panicle, corolla lavender or white, pod Long Circle or wealthy ligule, the flowering fruit bearing stage 5-10 month.Requiring not tight to growing environment, is Landscape Application high-quality seeds; Wood quality structure is fine and closely woven, is apparatus and equipment making material that high-quality negative G and pulling force are strong.Root micromicro is medicinal, has effect that is clearing heat and detoxicating, hemostasia and detumescence.Be distributed widely in all over China.
Because yellow wingceltis has a extensive future, market efficiency is remarkable, and demand grows with each passing day.But yellow wingceltis seed and the less and quality of seedling total amount mix, so adopt cuttage mode to breed to meet the need of market to it.
The method improving yellow wingceltis cuttage quality disclosed in prior art is mainly maternal plant plantation, cutting strunk is short to be sprouted, maternal plant manages, cuttings gathers, the method such as cuttage and management, main center of gravity is a control of cutting quality, cuttage survival rate can be improved to a certain extent, but cuttage final-period management aspect still adopts traditional type experience to manage, the step such as spray nutritious liquor and fertilising is all regular implementations, place can not be cultivated and environment is made real-time according to reality, accurate management, and require higher to the yellow wingceltis cuttage technique of administrative staff, so that carry out manual intervention in good time, this method subjectivity is larger, quality for yellow wingceltis cuttage seeding is difficult to ensure.And comparatively large to the restriction of cutting diameter range in traditional approach, mostly only gather diameter be about 1cm be rudiment cutting, limit cutting collecting quantity, be unfavorable for the scale breeding of yellow wingceltis.
Summary of the invention
In order to solve above technical problem, the invention provides following technical scheme:
A kind of yellow wingceltis cuttage and seedling culture method, comprises the following steps:
Step S1: substrate composition, yellow soil by volume: humus soil: peat soil: coconut palm chaff 3:3:2:2;
Step S2: seedbed pretreatment, before cuttage 1-2 days with before the formalin cuttage of 2% with 2% formalin sterilization;
Step S3: cutting collection, gathers the non-rudiment cutting that annual maternal plant upper diameter is 0.5-1.5cm the 7-8 month, with the length clip cutting of 6-8cm; Described maternal plant upper diameter is that the non-rudiment cutting of 0.5-1.5cm also will have certain degree of lignification, to meet the nutrient demand of cuttage root-taking.Gathering cutting when 7-8 month yellow wingceltis starts fallen leaves and do not take out sprouting is because now cutting endotrophic material abundance, is conducive to taking root and growing.
Step S4: cutting process, cutting adopts the processing method of quarter sawing, rip cutting two cutter, and the rip cutting degree of depth is 1-3cm; Use the thiophanate methyl solution disinfection of 0.15% afterwards; Quarter sawing comparatively truncation cutting heals early, and take root early, root of hair is many, and under experimental data proves equal external condition, quarter sawing improves 45% than truncation rooting rate, root bar sum increase by 38%.
Step S5: cuttage, after using the IBA solution of 150mg/L to soak, straight cutting enters in matrix container, and inserting the degree of depth of matrix is 2-3cm, keep blade not with base contact;
Step S6: integrated management, sets up total management system in seedling growth greenhouse, manages after cuttage completes to temp. and humidity and illumination etc.
Substrate composition acid-base value in step S1 is about 6.5, taking root and growth of suitable yellow wingceltis, and porosity is about 90%, and matrix is comparatively light, loose, and the amount holding air and water is large, and be conducive to root growth, unit weight is approximately 0.6g/cm 3, water binding capacity and air capacity of soils higher.Expand the scope of cutting diameter in step S3, by the Precision management of later stage to seedling growth greenhouse environment, the cutting making rugosity less also can well be taken root and be survived, and substantially increases the quantity of optional cutting, is conducive to the large-scaled propugation of yellow wingceltis, increases economic benefit.
Further, described total management system comprises temperature, humidity, illuminance acquisition module, temperature, humidity, illuminance control module, LCD MODULE, warning system, reset circuit and crystal oscillator module and single-chip microcomputer, described single-chip microcomputer connects each module respectively; Described temperature collect module is temperature sensor, be uniformly distributed in seedling growth greenhouse inside, described humidity collection module comprises air humidity sensor and soil humidity sensor, described illuminance acquisition module is photo resistance, described photo resistance is arranged at canopy wall, described temperature, humidity, illuminance control module comprise electric hot plate, sprayer and sunshade net, described electric hot plate is latticed, be embedded in below upper soll layer 3-5cm, cutting carries out cuttage according to the grid of electric hot plate, described sprayer is arranged at inside ceiling, and described sunshade net is arranged at outside ceiling.Utilize Single-chip Controlling Temperature Humidity Sensor, photo resistance collection seedling growth greenhouse temp. and humidity and illuminance, wherein air humidity and soil moisture separate detection, with accurate qualitative integrated environment humidity, the data feedback gathered is to single-chip microcomputer, and be presented on LCDs, when temp. and humidity and illuminance be not at the optimum range set, Single-chip Controlling warning system is reported to the police, simultaneously send information to control module, control module is carried out electric hot plate and to be heated or sprayer water spray or sunshade net cover.
Further, described maternal plant upper diameter is that the non-rudiment cutting of 0.5-1.5cm needs certain degree of lignification, to meet the nutrient demand of cuttage root-taking.
Further, described total management system also comprises a soil fertility detector, described soil fertility detector, for carrying out the detection of the soil organic matter and N P and K etc., mainly comprises Cation Exchange Capacity in Soils, full nitrogen and available nitrogen, the Indexs measure such as full phosphorus and rapid available phosphorus, and be connected with single-chip microcomputer, when fertility being detected lower than setting value, start warning system, be conducive to accurately monitoring soil fertility, ensure the nutrition supply of yellow wingceltis cuttage seeding.When warning system starts, artificial spray nutrient solution need be carried out or carry out automatic spraying by nutrient solution in proportion spray injection device.
Further, the collocation method of described nutrient solution for add 3-5mgIBA and 5-8mgNAA in 1 liter of MS nutrient solution, and use of being arranged in pairs or groups by IBA and NAA can significantly improve rooting rate and number of taking root.
Further, start to carry out to leak informaton, lower the temperature, control the hardening steps such as water after 60 days, being conducive to improving the later stage goes out the survival rate that canopy transplants.
Beneficial effect of the present invention is:
(1), expand the range of choice of cutting diameter, the optional 0.5cm of minimum diameter, and the rooting rate that can ensure the cutting that rugosity is less, add the quantity of optional cutting, be conducive to the scale breeding of yellow wingceltis, increases economic benefit.
(2), can according to reality cultivate place and environment make real-time, manage accurately, comprise the management of temp. and humidity, illuminance and soil fertility, wherein moisture management is also divided into air humidity management to manage with soil moisture, the environment of almost Perfect is provided to the rooting development of yellow wingceltis cuttage seeding, substantially increase rooting rate, number of taking root and root total length, and the cycle of taking root is short.
Embodiment
A kind of yellow wingceltis cuttage and seedling culture method, comprises the following steps:
Step S1: substrate composition, yellow soil by volume: humus soil: peat soil: coconut palm chaff=3:3:2:2;
Step S2: seedbed pretreatment, first 2 days of cuttage is with the formalin sterilization with 2% before the formalin cuttage of 2%;
Step S3: cutting collection, gathers the non-rudiment cutting that annual maternal plant upper diameter is 0.5-1.5cm the 7-8 month, with the length clip cutting of 6-8cm; Described maternal plant upper diameter is that the non-rudiment cutting of 0.5-1.5cm also will have certain degree of lignification, to meet the nutrient demand of cuttage root-taking.Gathering cutting when 7-8 month yellow wingceltis starts fallen leaves and do not take out sprouting is because now cutting endotrophic material abundance, is conducive to taking root and growing.
Step S4: cutting process, cutting adopts the processing method of quarter sawing, rip cutting two cutter, and the rip cutting degree of depth is 1-3cm; Use the thiophanate methyl solution disinfection of 0.15% afterwards; Quarter sawing comparatively truncation cutting heals early, and take root early, root of hair is many, and under experimental data proves equal external condition, quarter sawing improves 45% than truncation rooting rate, root bar sum increase by 38%.
Step S5: cuttage, after using the IBA solution of 150mg/L to soak, straight cutting enters in matrix container, inserting the degree of depth of matrix is 2-3cm, keep blade not with base contact; When experimental data proves IBA concentration at 150mg/L, rooting rate is the highest, is 93.7%.
Step S6: integrated management, sets up total management system in seedling growth greenhouse, manages after cuttage completes to temp. and humidity and illumination etc.
Substrate composition acid-base value in step S1 is about 6.5, taking root and growth of suitable yellow wingceltis, and porosity is about 90%, and matrix is comparatively light, loose, and the amount holding air and water is large, and be conducive to root growth, unit weight is approximately 0.6g/cm 3, water binding capacity and air capacity of soils higher.The scope of cutting diameter is expanded in step S3, by the Precision management of later stage to seedling growth greenhouse environment, the cutting making rugosity less also can well be taken root and be survived, and substantially increases the quantity of optional cutting, be conducive to the large-scaled propugation of this famous and precious seeds of yellow wingceltis, increase economic benefit.
Described total management system comprises temperature, humidity, illuminance acquisition module, temperature, humidity, illuminance control module, LCD MODULE, warning system, reset circuit and crystal oscillator module and single-chip microcomputer, and described single-chip microcomputer connects each module respectively; Described temperature collect module is temperature sensor, be uniformly distributed in seedling growth greenhouse inside, described humidity collection module comprises air humidity sensor and soil humidity sensor, described illuminance acquisition module is photo resistance, described photo resistance is arranged at canopy wall, described temperature, humidity, illuminance control module comprise electric hot plate, sprayer and sunshade net, described electric hot plate is latticed, be embedded in below upper soll layer 3-5cm, cutting carries out cuttage according to the grid of electric hot plate, described sprayer is arranged at inside ceiling, and described sunshade net is arranged at outside ceiling.Utilize Single-chip Controlling Temperature Humidity Sensor, photo resistance collection seedling growth greenhouse temp. and humidity and illuminance, wherein air humidity and soil moisture separate detection, with accurate qualitative integrated environment humidity, the data feedback gathered is to single-chip microcomputer, and be presented on LCDs, when temp. and humidity and illuminance be not at the optimum range set, Single-chip Controlling warning system is reported to the police, simultaneously send information to control module, control module is carried out electric hot plate and to be heated or sprayer water spray or sunshade net cover.
Described total management system also comprises a soil fertility detector, described soil fertility detector is for carrying out the detection of the soil organic matter and N P and K etc., mainly comprise Cation Exchange Capacity in Soils, full nitrogen and available nitrogen, the Indexs measure such as full phosphorus and rapid available phosphorus, and be connected with single-chip microcomputer, when fertility being detected lower than setting value, start warning system, be conducive to accurately monitoring soil fertility, ensure the nutrition supply of yellow wingceltis cuttage seeding.When warning system starts, artificial spray nutrient solution need be carried out or carry out automatic spraying by nutrient solution in proportion spray injection device.
The collocation method of described nutrient solution for add 5mgIBA and 5mgNAA in 1 liter of MS nutrient solution, and use of being arranged in pairs or groups by IBA and NAA can significantly improve rooting rate and number of taking root.
Cuttage starts after completing 60 days to carry out to leak informaton, lowers the temperature, controls the hardening steps such as water, is conducive to improving the later stage to go out the survival rate that canopy transplants.
Show through experimental data, existing cuttage and seedling culture survival rate fluctuates along with the change of external environment condition and final-period management level between 60-90%, and rooting rate can control more than 92% by yellow wingceltis cuttage and seedling culture method of the present invention substantially, the later stage, survival rate was to more than 90%.
With above-mentioned foundation desirable embodiment of the present invention for enlightenment, by above-mentioned description, relevant staff in the scope not departing from this invention technological thought, can carry out various change and amendment completely.The technical scope of this invention is not limited to the content on specification, must determine its technical scope according to right.

Claims (7)

1. a yellow wingceltis cuttage and seedling culture method, is characterized in that, comprises the following steps:
Step S1: substrate composition, yellow soil by volume: humus soil: peat soil: coconut palm chaff=3:3:2:2;
Step S2: seedbed pretreatment, before cuttage 1-2 days with before the formalin cuttage of 2% with 2% formalin sterilization;
Step S3: cutting collection, gathers the non-rudiment cutting that annual maternal plant upper diameter is 0.5-1.5cm the 7-8 month, with the length clip cutting of 6-8cm;
Step S4: cutting process, cutting adopts the processing method of quarter sawing, rip cutting two cutter, and the rip cutting degree of depth is 1-3cm; Use the thiophanate methyl solution disinfection of 0.15% afterwards;
Step S5: cuttage, after using the IBA solution of 150mg/L to soak, straight cutting enters in matrix container, inserting the degree of depth of matrix is 2-3cm, keep blade not with base contact;
Step S6: integrated management, sets up total management system in seedling growth greenhouse, manages after cuttage completes to temp. and humidity and illumination etc.
2. a kind of yellow wingceltis cuttage and seedling culture method as claimed in claim 1, it is characterized in that: described total management system comprises temperature, humidity, illuminance acquisition module, temperature, humidity, illuminance control module, LCD MODULE, warning system, reset circuit and crystal oscillator module and single-chip microcomputer, described single-chip microcomputer connects each module respectively; Described temperature collect module is temperature sensor, be uniformly distributed in seedling growth greenhouse inside, described humidity collection module comprises air humidity sensor and soil humidity sensor, described illuminance acquisition module is photo resistance, described photo resistance is arranged at canopy wall, described temperature, humidity, illuminance control module comprise electric hot plate, sprayer and sunshade net, described electric hot plate is latticed, be embedded in below upper soll layer 3-5cm, cutting carries out cuttage according to the grid of electric hot plate, described sprayer is arranged at inside ceiling, and described sunshade net is arranged at outside ceiling.
3. a kind of yellow wingceltis cuttage and seedling culture method as claimed in claim 1, is characterized in that: described maternal plant upper diameter is that the non-rudiment cutting of 1.2cm needs certain degree of lignification, to meet the nutrient demand of cuttage root-taking.
4. a kind of yellow wingceltis cuttage and seedling culture method as claimed in claim 1, it is characterized in that: described total management system also comprises a soil fertility detector, described soil fertility detector is for carrying out the detection of the soil organic matter and N P and K etc., and be connected with single-chip microcomputer, when fertility being detected lower than setting value, start warning system.
5. a kind of yellow wingceltis cuttage and seedling culture method as claimed in claim 4, is characterized in that: when warning system starts, and need carry out artificial spray nutrient solution or carry out automatic spraying by nutrient solution in proportion spray injection device.
6. a kind of yellow wingceltis cuttage and seedling culture method as claimed in claim 5, is characterized in that: the collocation method of described nutrient solution for add 3-5mgIBA and 5-8mgNAA in 1 liter of MS nutrient solution.
7. a kind of yellow wingceltis cuttage and seedling culture method as claimed in claim 1, is characterized in that: start after 60 days to carry out to leak informaton, lower the temperature, control the hardening steps such as water.
CN201510815833.8A 2015-11-20 2015-11-20 Cuttage propagation method for Dalbergia hupeana Hance Pending CN105325252A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105830796A (en) * 2016-03-31 2016-08-10 阮积恩 Dalbergia odorifera seedling culture substrate and preparation method thereof
CN108605798A (en) * 2016-11-30 2018-10-02 丹阳市大丰收苗木专业合作社 A kind of yellow wingceltis cutting medium
CN108703147A (en) * 2018-06-29 2018-10-26 中国医学科学院药用植物研究所海南分所 A kind of dalbergia odorifera plantation water-retaining agent, preparation method and applications
CN109197522A (en) * 2018-09-19 2019-01-15 海南大学 A kind of seedling medium and application promoting dalbergia odorifera growth of seedling and root system development
CN110959457A (en) * 2019-12-20 2020-04-07 漳州市林业科技推广站 Cutting seedling raising method for dalbergia odorifera

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102783349A (en) * 2012-08-29 2012-11-21 广西壮族自治区林业科学研究院 Method for improving breeding quality of dalbergia odorifera cuttings
CN103641599A (en) * 2013-11-25 2014-03-19 贵港市科隆农林高新技术开发有限公司 Sandalwood cultivation medium and preparation method thereof
CN105009927A (en) * 2015-08-26 2015-11-04 龚卫新 Cutting propagation method for controlling Hainan dalbergia odorifera diseases and pests

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102783349A (en) * 2012-08-29 2012-11-21 广西壮族自治区林业科学研究院 Method for improving breeding quality of dalbergia odorifera cuttings
CN103641599A (en) * 2013-11-25 2014-03-19 贵港市科隆农林高新技术开发有限公司 Sandalwood cultivation medium and preparation method thereof
CN105009927A (en) * 2015-08-26 2015-11-04 龚卫新 Cutting propagation method for controlling Hainan dalbergia odorifera diseases and pests

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
施福军等: "降香黄檀扦插繁殖技术研究", 《广东农业科学》 *
田芳明等: "基于单片机的低功耗水稻育秧棚监控系统设计", 《农 机 化 研 究》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105830796A (en) * 2016-03-31 2016-08-10 阮积恩 Dalbergia odorifera seedling culture substrate and preparation method thereof
CN108605798A (en) * 2016-11-30 2018-10-02 丹阳市大丰收苗木专业合作社 A kind of yellow wingceltis cutting medium
CN108703147A (en) * 2018-06-29 2018-10-26 中国医学科学院药用植物研究所海南分所 A kind of dalbergia odorifera plantation water-retaining agent, preparation method and applications
CN109197522A (en) * 2018-09-19 2019-01-15 海南大学 A kind of seedling medium and application promoting dalbergia odorifera growth of seedling and root system development
CN110959457A (en) * 2019-12-20 2020-04-07 漳州市林业科技推广站 Cutting seedling raising method for dalbergia odorifera

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