CN105188357B - 表达限制的免疫球蛋白轻链库的小鼠 - Google Patents

表达限制的免疫球蛋白轻链库的小鼠 Download PDF

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Publication number
CN105188357B
CN105188357B CN201480013894.6A CN201480013894A CN105188357B CN 105188357 B CN105188357 B CN 105188357B CN 201480013894 A CN201480013894 A CN 201480013894A CN 105188357 B CN105188357 B CN 105188357B
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China
Prior art keywords
human
light chain
mouse
reset
hig
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English (en)
Chinese (zh)
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CN105188357A (zh
Inventor
R·巴布
J·麦克维尔特
L·麦克唐纳
S·史蒂文斯
S·戴维斯
D·R·巴克勒
K·A·米盖尔
A·J·墨菲
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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Priority claimed from US13/798,455 external-priority patent/US9796788B2/en
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Priority to CN201810439928.8A priority Critical patent/CN108611369B/zh
Publication of CN105188357A publication Critical patent/CN105188357A/zh
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480013894.6A 2013-03-13 2014-03-13 表达限制的免疫球蛋白轻链库的小鼠 Active CN105188357B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810439928.8A CN108611369B (zh) 2013-03-13 2014-03-13 表达限制的免疫球蛋白轻链库的小鼠

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/798,455 2013-03-13
US13/798,455 US9796788B2 (en) 2010-02-08 2013-03-13 Mice expressing a limited immunoglobulin light chain repertoire
PCT/US2014/026040 WO2014160202A1 (en) 2013-03-13 2014-03-13 Mice expressing a limited immunoglobulin light chain repertoire

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201810439928.8A Division CN108611369B (zh) 2013-03-13 2014-03-13 表达限制的免疫球蛋白轻链库的小鼠

Publications (2)

Publication Number Publication Date
CN105188357A CN105188357A (zh) 2015-12-23
CN105188357B true CN105188357B (zh) 2018-04-24

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CN201480013894.6A Active CN105188357B (zh) 2013-03-13 2014-03-13 表达限制的免疫球蛋白轻链库的小鼠
CN201810439928.8A Active CN108611369B (zh) 2013-03-13 2014-03-13 表达限制的免疫球蛋白轻链库的小鼠

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EP (4) EP2967013B1 (enExample)
JP (4) JP6529957B2 (enExample)
KR (2) KR102295842B1 (enExample)
CN (2) CN105188357B (enExample)
AU (3) AU2014244020B2 (enExample)
BR (1) BR112015022034B1 (enExample)
CA (1) CA2903698C (enExample)
CY (1) CY1121545T1 (enExample)
DK (2) DK2967013T3 (enExample)
ES (3) ES3013063T3 (enExample)
FI (1) FI3501272T3 (enExample)
HR (2) HRP20230490T1 (enExample)
HU (2) HUE061977T2 (enExample)
IL (2) IL240563B (enExample)
LT (2) LT2967013T (enExample)
MX (3) MX357308B (enExample)
PL (2) PL3501272T3 (enExample)
PT (2) PT2967013T (enExample)
RS (2) RS58669B1 (enExample)
RU (1) RU2689664C2 (enExample)
SG (2) SG11201506278RA (enExample)
SI (2) SI2967013T1 (enExample)
SM (2) SMT202300112T1 (enExample)
TR (1) TR201901668T4 (enExample)
WO (1) WO2014160202A1 (enExample)
ZA (2) ZA201505835B (enExample)

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US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
ES2872081T3 (es) 2011-08-05 2021-11-02 Regeneron Pharma Ratones con cadena ligera universal humanizada
EP4074833A1 (en) 2011-10-17 2022-10-19 Regeneron Pharmaceuticals, Inc. Restricted immunoglobulin heavy chain mice
NZ703689A (en) 2012-06-12 2016-05-27 Regeneron Pharma Humanized non-human animals with restricted immunoglobulin heavy chain loci
RS57582B1 (sr) * 2013-02-20 2018-11-30 Regeneron Pharma Nehumane životinje sa modifikovanim sekvencama imunoglobulinskog teškog lanca
AU2015230938C1 (en) 2014-03-21 2021-10-14 Regeneron Pharmaceuticals, Inc. Non-human animals that make single domain binding proteins
CN107438622A (zh) 2015-03-19 2017-12-05 瑞泽恩制药公司 选择结合抗原的轻链可变区的非人动物
US20190037816A1 (en) 2016-01-13 2019-02-07 Regeneron Pharmaceuticals, Inc. Rodents having an engineered heavy chain diversity region
BR112020004977A2 (pt) * 2017-09-29 2020-10-06 Regeneron Pharmaceuticals, Inc. moléculas de ligação de antígenos biespecíficas que ligam antígeno alvo de staphyocococcus e componente de complemento e usos dos mesmos
IL318469A (en) 2018-06-14 2025-03-01 Regeneron Pharma Non-human animals capable of reorganizing transgenic DH-DH, and their uses
EP3897117A1 (en) * 2018-12-21 2021-10-27 Compass Therapeutics LLC Transgenic mouse expressing common human light chain
WO2020169022A1 (en) 2019-02-18 2020-08-27 Beijing Biocytogen Co., Ltd Genetically modified non-human animals with humanized immunoglobulin locus
BR112021016173A2 (pt) 2019-02-22 2021-11-03 Regeneron Pharma Roedor geneticamente modificado, métodos de produção de um roedor geneticamente modificado e de produção de um anticorpo anti-nav1.7, célula ou tecido isolado de roedor, linhagem celular imortalizada, embrião de roedor, construto de ácido nucleico de direcionamento, e, hibridoma
KR20210095781A (ko) 2020-01-24 2021-08-03 주식회사 에이프릴바이오 항원결합 단편 및 생리활성 이펙터 모이어티로 구성된 융합 컨스트럭트를 포함하는 다중결합항체 및 이를 포함하는 약학조성물
CN115667532A (zh) 2020-06-02 2023-01-31 百奥赛图(北京)医药科技股份有限公司 具有共有轻链免疫球蛋白基因座的经遗传修饰的非人动物
CA3197426A1 (en) 2020-12-23 2022-06-30 Regeneron Pharmaceuticals, Inc. Methods for obtaining antibodies that bind transmembrane proteins and cells that produce the same
MX2023007401A (es) 2020-12-23 2023-07-06 Regeneron Pharma Acidos nucleicos que codifican anticuerpos modificados por anclaje y usos de los mismos.
CN114736927A (zh) * 2022-05-19 2022-07-12 东北大学 一种单抗克隆与表达载体的构建及应用
TW202615661A (zh) * 2023-01-18 2026-04-16 美商基利科學股份有限公司 人類免疫球蛋白二元輕鏈轉殖基因構築體及其用途
US20260022395A1 (en) * 2024-07-16 2026-01-22 Gilead Sciences, Inc. Human immunoglobulin common light chain transgene constructs and uses thereof
CN121628915A (zh) * 2024-08-29 2026-03-10 上海泰槿生物技术有限公司 产生限制的免疫球蛋白轻链库的细胞、动物和方法

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Publication number Publication date
EP4218409B1 (en) 2025-02-12
CN108611369B (zh) 2022-08-26
CA2903698C (en) 2023-02-28
JP2016512034A (ja) 2016-04-25
SMT201900196T1 (it) 2019-05-10
HK1218493A1 (en) 2017-02-24
KR20210107911A (ko) 2021-09-01
SI2967013T1 (sl) 2019-03-29
MX377146B (es) 2025-03-07
KR20150126871A (ko) 2015-11-13
RU2015143110A (ru) 2017-04-18
WO2014160202A1 (en) 2014-10-02
BR112015022034B1 (pt) 2023-02-28
PL3501272T3 (pl) 2023-07-03
EP3501272A1 (en) 2019-06-26
RS58669B1 (sr) 2019-06-28
RU2689664C2 (ru) 2019-05-28
IL240563B (en) 2021-03-25
KR102424756B1 (ko) 2022-07-25
IL280892B1 (en) 2023-01-01
JP2019076109A (ja) 2019-05-23
EP4218409C0 (en) 2025-02-12
SG10201801101QA (en) 2018-04-27
EP4545567A3 (en) 2025-08-13
HUE044003T2 (hu) 2019-09-30
TR201901668T4 (tr) 2019-02-21
IL280892A (en) 2021-04-29
AU2020203238A1 (en) 2020-06-11
DK2967013T3 (en) 2019-03-11
PT2967013T (pt) 2019-04-03
ES3013063T3 (en) 2025-04-10
MX2020010837A (es) 2022-03-28
JP6529957B2 (ja) 2019-06-12
AU2020203238B2 (en) 2023-04-13
IL280892B2 (en) 2023-05-01
MX2015012539A (es) 2016-06-28
CN108611369A (zh) 2018-10-02
DK3501272T3 (da) 2023-04-03
CN105188357A (zh) 2015-12-23
LT3501272T (lt) 2023-03-27
JP7010902B2 (ja) 2022-02-10
AU2014244020B2 (en) 2020-06-04
LT2967013T (lt) 2019-04-25
MX391010B (es) 2025-03-21
SG11201506278RA (en) 2015-09-29
KR102295842B1 (ko) 2021-08-31
ZA201505835B (en) 2019-09-25
BR112015022034A2 (pt) 2017-08-29
SI3501272T1 (sl) 2023-05-31
AU2023202783A1 (en) 2023-05-25
ES2712207T3 (es) 2019-05-09
AU2014244020A1 (en) 2015-09-24
JP2020182490A (ja) 2020-11-12
FI3501272T3 (fi) 2023-04-06
NZ711776A (en) 2021-09-24
ES2942413T3 (es) 2023-06-01
SMT202300112T1 (it) 2023-05-12
EP4545567A2 (en) 2025-04-30
EP4218409A2 (en) 2023-08-02
WO2014160202A4 (en) 2014-11-13
JP6790136B2 (ja) 2020-11-25
RU2019115237A (ru) 2019-06-11
EP4218409A3 (en) 2023-08-30
AU2023202783B2 (en) 2026-02-12
JP2019213549A (ja) 2019-12-19
EP3501272B1 (en) 2023-03-01
EP2967013A1 (en) 2016-01-20
DK3501272T5 (da) 2024-09-02
ZA201805369B (en) 2021-08-25
HUE061977T2 (hu) 2023-09-28
HRP20190706T1 (hr) 2019-10-18
HRP20230490T1 (hr) 2023-08-04
IL240563A0 (en) 2015-09-24
PL2967013T3 (pl) 2019-07-31
CY1121545T1 (el) 2020-05-29
RS64185B1 (sr) 2023-05-31
PT3501272T (pt) 2023-03-29
EP2967013B1 (en) 2019-01-16
MX357308B (es) 2018-07-04
CA2903698A1 (en) 2014-10-02

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