AU2014244020B2 - Mice expressing a limited immunoglobulin light chain repertoire - Google Patents

Mice expressing a limited immunoglobulin light chain repertoire Download PDF

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Publication number
AU2014244020B2
AU2014244020B2 AU2014244020A AU2014244020A AU2014244020B2 AU 2014244020 B2 AU2014244020 B2 AU 2014244020B2 AU 2014244020 A AU2014244020 A AU 2014244020A AU 2014244020 A AU2014244020 A AU 2014244020A AU 2014244020 B2 AU2014244020 B2 AU 2014244020B2
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AU
Australia
Prior art keywords
human
mouse
light chain
immunoglobulin
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2014244020A
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English (en)
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AU2014244020A1 (en
Inventor
Lynn Macdonald
John Mcwhirter
Andrew J. Murphy
Sean Stevens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/798,455 external-priority patent/US9796788B2/en
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of AU2014244020A1 publication Critical patent/AU2014244020A1/en
Priority to AU2020203238A priority Critical patent/AU2020203238B2/en
Application granted granted Critical
Publication of AU2014244020B2 publication Critical patent/AU2014244020B2/en
Priority to AU2023202783A priority patent/AU2023202783B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2014244020A 2013-03-13 2014-03-13 Mice expressing a limited immunoglobulin light chain repertoire Active AU2014244020B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2020203238A AU2020203238B2 (en) 2013-03-13 2020-05-19 Mice expressing a limited immunoglobulin light chain repertoire
AU2023202783A AU2023202783B2 (en) 2013-03-13 2023-05-04 Mice expressing a limited immunoglobulin light chain repertoire

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/798,455 2013-03-13
US13/798,455 US9796788B2 (en) 2010-02-08 2013-03-13 Mice expressing a limited immunoglobulin light chain repertoire
PCT/US2014/026040 WO2014160202A1 (en) 2013-03-13 2014-03-13 Mice expressing a limited immunoglobulin light chain repertoire

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2020203238A Division AU2020203238B2 (en) 2013-03-13 2020-05-19 Mice expressing a limited immunoglobulin light chain repertoire

Publications (2)

Publication Number Publication Date
AU2014244020A1 AU2014244020A1 (en) 2015-09-24
AU2014244020B2 true AU2014244020B2 (en) 2020-06-04

Family

ID=50483561

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2014244020A Active AU2014244020B2 (en) 2013-03-13 2014-03-13 Mice expressing a limited immunoglobulin light chain repertoire
AU2020203238A Active AU2020203238B2 (en) 2013-03-13 2020-05-19 Mice expressing a limited immunoglobulin light chain repertoire
AU2023202783A Active AU2023202783B2 (en) 2013-03-13 2023-05-04 Mice expressing a limited immunoglobulin light chain repertoire

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2020203238A Active AU2020203238B2 (en) 2013-03-13 2020-05-19 Mice expressing a limited immunoglobulin light chain repertoire
AU2023202783A Active AU2023202783B2 (en) 2013-03-13 2023-05-04 Mice expressing a limited immunoglobulin light chain repertoire

Country Status (26)

Country Link
EP (4) EP2967013B1 (enExample)
JP (4) JP6529957B2 (enExample)
KR (2) KR102295842B1 (enExample)
CN (2) CN105188357B (enExample)
AU (3) AU2014244020B2 (enExample)
BR (1) BR112015022034B1 (enExample)
CA (1) CA2903698C (enExample)
CY (1) CY1121545T1 (enExample)
DK (2) DK2967013T3 (enExample)
ES (3) ES3013063T3 (enExample)
FI (1) FI3501272T3 (enExample)
HR (2) HRP20230490T1 (enExample)
HU (2) HUE061977T2 (enExample)
IL (2) IL240563B (enExample)
LT (2) LT2967013T (enExample)
MX (3) MX357308B (enExample)
PL (2) PL3501272T3 (enExample)
PT (2) PT2967013T (enExample)
RS (2) RS58669B1 (enExample)
RU (1) RU2689664C2 (enExample)
SG (2) SG11201506278RA (enExample)
SI (2) SI2967013T1 (enExample)
SM (2) SMT202300112T1 (enExample)
TR (1) TR201901668T4 (enExample)
WO (1) WO2014160202A1 (enExample)
ZA (2) ZA201505835B (enExample)

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SG183149A1 (en) 2010-02-08 2012-09-27 Regeneron Pharma Common light chain mouse
US9796788B2 (en) 2010-02-08 2017-10-24 Regeneron Pharmaceuticals, Inc. Mice expressing a limited immunoglobulin light chain repertoire
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
ES2872081T3 (es) 2011-08-05 2021-11-02 Regeneron Pharma Ratones con cadena ligera universal humanizada
EP4074833A1 (en) 2011-10-17 2022-10-19 Regeneron Pharmaceuticals, Inc. Restricted immunoglobulin heavy chain mice
NZ703689A (en) 2012-06-12 2016-05-27 Regeneron Pharma Humanized non-human animals with restricted immunoglobulin heavy chain loci
RS57582B1 (sr) * 2013-02-20 2018-11-30 Regeneron Pharma Nehumane životinje sa modifikovanim sekvencama imunoglobulinskog teškog lanca
AU2015230938C1 (en) 2014-03-21 2021-10-14 Regeneron Pharmaceuticals, Inc. Non-human animals that make single domain binding proteins
CN107438622A (zh) 2015-03-19 2017-12-05 瑞泽恩制药公司 选择结合抗原的轻链可变区的非人动物
US20190037816A1 (en) 2016-01-13 2019-02-07 Regeneron Pharmaceuticals, Inc. Rodents having an engineered heavy chain diversity region
BR112020004977A2 (pt) * 2017-09-29 2020-10-06 Regeneron Pharmaceuticals, Inc. moléculas de ligação de antígenos biespecíficas que ligam antígeno alvo de staphyocococcus e componente de complemento e usos dos mesmos
IL318469A (en) 2018-06-14 2025-03-01 Regeneron Pharma Non-human animals capable of reorganizing transgenic DH-DH, and their uses
EP3897117A1 (en) * 2018-12-21 2021-10-27 Compass Therapeutics LLC Transgenic mouse expressing common human light chain
WO2020169022A1 (en) 2019-02-18 2020-08-27 Beijing Biocytogen Co., Ltd Genetically modified non-human animals with humanized immunoglobulin locus
BR112021016173A2 (pt) 2019-02-22 2021-11-03 Regeneron Pharma Roedor geneticamente modificado, métodos de produção de um roedor geneticamente modificado e de produção de um anticorpo anti-nav1.7, célula ou tecido isolado de roedor, linhagem celular imortalizada, embrião de roedor, construto de ácido nucleico de direcionamento, e, hibridoma
KR20210095781A (ko) 2020-01-24 2021-08-03 주식회사 에이프릴바이오 항원결합 단편 및 생리활성 이펙터 모이어티로 구성된 융합 컨스트럭트를 포함하는 다중결합항체 및 이를 포함하는 약학조성물
CN115667532A (zh) 2020-06-02 2023-01-31 百奥赛图(北京)医药科技股份有限公司 具有共有轻链免疫球蛋白基因座的经遗传修饰的非人动物
CA3197426A1 (en) 2020-12-23 2022-06-30 Regeneron Pharmaceuticals, Inc. Methods for obtaining antibodies that bind transmembrane proteins and cells that produce the same
MX2023007401A (es) 2020-12-23 2023-07-06 Regeneron Pharma Acidos nucleicos que codifican anticuerpos modificados por anclaje y usos de los mismos.
CN114736927A (zh) * 2022-05-19 2022-07-12 东北大学 一种单抗克隆与表达载体的构建及应用
TW202615661A (zh) * 2023-01-18 2026-04-16 美商基利科學股份有限公司 人類免疫球蛋白二元輕鏈轉殖基因構築體及其用途
US20260022395A1 (en) * 2024-07-16 2026-01-22 Gilead Sciences, Inc. Human immunoglobulin common light chain transgene constructs and uses thereof
CN121628915A (zh) * 2024-08-29 2026-03-10 上海泰槿生物技术有限公司 产生限制的免疫球蛋白轻链库的细胞、动物和方法

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WO2013022782A1 (en) * 2011-08-05 2013-02-14 Regeneron Pharmaceuticals, Inc. Humanized universal light chain mice
US20130045492A1 (en) * 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain

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WO2012148873A2 (en) * 2011-04-25 2012-11-01 Regeneron Pharmaceuticals, Inc. Non-human animals expressing antibodies having a common light chain
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Also Published As

Publication number Publication date
EP4218409B1 (en) 2025-02-12
CN108611369B (zh) 2022-08-26
CA2903698C (en) 2023-02-28
JP2016512034A (ja) 2016-04-25
SMT201900196T1 (it) 2019-05-10
HK1218493A1 (en) 2017-02-24
KR20210107911A (ko) 2021-09-01
SI2967013T1 (sl) 2019-03-29
MX377146B (es) 2025-03-07
KR20150126871A (ko) 2015-11-13
RU2015143110A (ru) 2017-04-18
WO2014160202A1 (en) 2014-10-02
BR112015022034B1 (pt) 2023-02-28
PL3501272T3 (pl) 2023-07-03
EP3501272A1 (en) 2019-06-26
RS58669B1 (sr) 2019-06-28
RU2689664C2 (ru) 2019-05-28
IL240563B (en) 2021-03-25
KR102424756B1 (ko) 2022-07-25
IL280892B1 (en) 2023-01-01
JP2019076109A (ja) 2019-05-23
EP4218409C0 (en) 2025-02-12
SG10201801101QA (en) 2018-04-27
EP4545567A3 (en) 2025-08-13
HUE044003T2 (hu) 2019-09-30
TR201901668T4 (tr) 2019-02-21
IL280892A (en) 2021-04-29
AU2020203238A1 (en) 2020-06-11
DK2967013T3 (en) 2019-03-11
PT2967013T (pt) 2019-04-03
ES3013063T3 (en) 2025-04-10
MX2020010837A (es) 2022-03-28
CN105188357B (zh) 2018-04-24
JP6529957B2 (ja) 2019-06-12
AU2020203238B2 (en) 2023-04-13
IL280892B2 (en) 2023-05-01
MX2015012539A (es) 2016-06-28
CN108611369A (zh) 2018-10-02
DK3501272T3 (da) 2023-04-03
CN105188357A (zh) 2015-12-23
LT3501272T (lt) 2023-03-27
JP7010902B2 (ja) 2022-02-10
LT2967013T (lt) 2019-04-25
MX391010B (es) 2025-03-21
SG11201506278RA (en) 2015-09-29
KR102295842B1 (ko) 2021-08-31
ZA201505835B (en) 2019-09-25
BR112015022034A2 (pt) 2017-08-29
SI3501272T1 (sl) 2023-05-31
AU2023202783A1 (en) 2023-05-25
ES2712207T3 (es) 2019-05-09
AU2014244020A1 (en) 2015-09-24
JP2020182490A (ja) 2020-11-12
FI3501272T3 (fi) 2023-04-06
NZ711776A (en) 2021-09-24
ES2942413T3 (es) 2023-06-01
SMT202300112T1 (it) 2023-05-12
EP4545567A2 (en) 2025-04-30
EP4218409A2 (en) 2023-08-02
WO2014160202A4 (en) 2014-11-13
JP6790136B2 (ja) 2020-11-25
RU2019115237A (ru) 2019-06-11
EP4218409A3 (en) 2023-08-30
AU2023202783B2 (en) 2026-02-12
JP2019213549A (ja) 2019-12-19
EP3501272B1 (en) 2023-03-01
EP2967013A1 (en) 2016-01-20
DK3501272T5 (da) 2024-09-02
ZA201805369B (en) 2021-08-25
HUE061977T2 (hu) 2023-09-28
HRP20190706T1 (hr) 2019-10-18
HRP20230490T1 (hr) 2023-08-04
IL240563A0 (en) 2015-09-24
PL2967013T3 (pl) 2019-07-31
CY1121545T1 (el) 2020-05-29
RS64185B1 (sr) 2023-05-31
PT3501272T (pt) 2023-03-29
EP2967013B1 (en) 2019-01-16
MX357308B (es) 2018-07-04
CA2903698A1 (en) 2014-10-02

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