CN105168811B - A kind of Chinese medicine soluble powder for preventing broiler chicken chicken infectious provenculitis-ventriculitis and preparation method thereof - Google Patents
A kind of Chinese medicine soluble powder for preventing broiler chicken chicken infectious provenculitis-ventriculitis and preparation method thereof Download PDFInfo
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- CN105168811B CN105168811B CN201510487010.7A CN201510487010A CN105168811B CN 105168811 B CN105168811 B CN 105168811B CN 201510487010 A CN201510487010 A CN 201510487010A CN 105168811 B CN105168811 B CN 105168811B
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Abstract
The present invention relates to a kind of Chinese medicine soluble powder for preventing broiler chicken chicken infectious provenculitis ventriculitis and preparation method thereof, it is made of the Chinese medicine material of following weight percentage：Radix astragali, Radix Codonopsis, honey-fried licorice root, rhizoma anemarrhenae, kuh-seng, radix scutellariae, Cortex Phellodendri, cape jasmine, cuttle bone each 8%, rhizoma atractylodis, process Chinese herbaceous peony, Fructus Forsythiae, radix scrophulariae, the coptis each 4.8%, dried orange peel 4%.Soluble powder is made after decocting, filter, freeze, crush.The Chinese medicine preparation of the present invention can effectively prevent the occurrence and development of broiler chicken chicken infectious provenculitis ventriculitis, treat both principal and secondary aspect of disease, cure rate height, and it is convenient to offer medicine, and does not develop immunity to drugs, drug residue free；Broiler chicken gastrointestinal function and immunocompetence can be improved, reduces the death rate, reduces feedstuff-meat ratio, the advantages of heavy is delivered in raising for sale.
Description
Technical field
It is further one the present invention relates to the Chinese medicine soluble powder of technical field of pharmaceuticals, specifically birds
Chinese medicine soluble powder of kind prevention broiler chicken chicken infectious provenculitis-ventriculitis and preparation method thereof.
Background technology
Since two thousand six, the white meat-type chickens on the Liaoning in China, Shandong, Hebei, Jilin, Heilungkiang, Henan and other places are supported
Grow City Regions occur successively broiler chicken feed intake is low, slow-growing, chicken feet turns white, glandular stomach wall thickening, glandular stomach nipple enlargement, seriously
The appearance symptom such as the membrane of a chicken's gizzard ulcer based on a kind of disease.Animal and veterinary practitioner is finally uniformly named as broiler chicken infectiousness
Proventriculitis (Infectious Proventriculitis, IP).The sick and its secondary ventriculitis (Muscle Gastritis)
It is very big to broiler chicken harm, feedstuff-meat ratio is improved, reduces the premunition of broiler chicken.
The fact that relevant animal medicine manufacturer is for acidity extreme rise in the sick broiler chicken gastric juice is suffered from, release
Chicken infectious provenculitis medicine, the compound preparation formed more with Cimetidine, antibiotic, antiviral drugs, reparation mucous membrane agent, but only
It can play the role of delaying the development of broiler chicken chicken infectious provenculitis, it is impossible to which the obvious obstruction broiler chicken chicken infectious provenculitis that reaches is sent out
The effect of exhibition.But from March, 2014《The Ministry of Agriculture of the People's Republic of China, MOA announces No. 2071》Since file performs, forbid " changing
Prescription adds other veterinary drug components " regulation so that treatment broiler chicken chicken infectious provenculitis effect of drugs decline, cause infection
Harm of the sexual gland gastritis to broiler chicken is increasing, and serious economic loss is caused to broiler breeding industry.
Since IP has the characteristics of morbidity is early, and the duration is long, brought a lot of trouble to prevention.Medicine is that prevention broiler chicken passes
The main means of metachromia proventriculitis-ventriculitis, in order to effectively prevent and control the sick generation, broiler breeding field (family) at present
Prevention to broiler chicken chicken infectious provenculitis-ventriculitis has all put into substantial amounts of fund.Due to broiler chicken chicken infectious provenculitis-ventriculitis
Cause of disease or the cause of disease diversity (uncertainty), without effective prevention method.Existing protective agents are primarily directed to meat
Avian infectious proventriculitis, but the occurrence and development to ventriculitis are without effectively preventing method.
Traditional Chinese veterinary medicine is of extensive knowledge and profound scholarship, and treatment disease is particular about entirety and treats both principal and secondary aspect of disease, and Chinese medicine is due to its efficient, low medicament residue
More and more preventions for being applied to animal epidemic, therefore, work out a Chinese prescription to prevent broiler chicken and infect sexual gland
Gastritis-ventriculitis becomes a main task of veterinary industry.Although Chinese medicine there has been substantial amounts of research work for the prevention of IP
Make, but it has been reported that prescription in it is more based on powder, dispensing is inconvenient, and affects the treatment, to broiler chicken chicken infectious provenculitis-flesh
Gastritis-controlling has certain difficulty, therefore, develop collection symptomatic treatment, antitoxin, antibacterial, antiulcer, it is efficient, nontoxic, easy to collecting
Reduction cultivation is used in integral new protective agents tool and is of great significance.
Since 2008, technical specialist of the first invention people as certain domestic well-known animal health-care product enterprise, is mainly responsible for
The technological service in broiler chicken market.The case of substantial amounts of broiler chicken chicken infectious provenculitis-ventriculitis has been cured using Chinese medicinal formulae.Learning
The research for the Henan Department of Education of Shanxi Province scientific and technical research key project that school carries out, it was found that chicken infectious provenculitis induces ventriculitis
The mechanism that gastric acid secretion increases, and cause muscular stomach class cutin membrane self-protection power decline the reason for.Having explored can be with antagonism
The Chinese medicinal formulae of factor is stated to prevent broiler chicken chicken infectious provenculitis-ventriculitis.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, and provide one kind treat both principal and secondary aspect of disease, cure rate it is high, and offer medicine
It is convenient, do not develop immunity to drugs, drug residue free；Broiler chicken gastrointestinal function and immunocompetence can be improved, reduces the death rate, reduces material meat
Than improving Chinese medicine soluble powder of a kind of prevention broiler chicken chicken infectious provenculitis-ventriculitis for delivering weight for sale and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is：
A kind of Chinese medicine soluble powder for preventing broiler chicken chicken infectious provenculitis-ventriculitis, it is by following weight percentage
Medicine raw material is made：Radix astragali, Radix Codonopsis, honey-fried licorice root, rhizoma anemarrhenae, kuh-seng, radix scutellariae, Cortex Phellodendri, cape jasmine, cuttle bone each 8%, rhizoma atractylodis, process Chinese herbaceous peony,
Fructus Forsythiae, radix scrophulariae, the coptis each 4.8%, dried orange peel 4%.
Present invention also offers the preparation method of above-mentioned Chinese medicine preparation, comprise the following steps：Chinese medicine is weighed by weight ratio
1 part of raw material, decocts twice.Decoct for the first time：1 part of Chinese medicine, 8 parts of water, soaks 30min, boils by intense fire, be changed to be cooked by slow fire
60min, filtering liquid medicine；Second of decoction：Add 6 parts of water in the first time dregs of a decoction, boil by intense fire, be changed to be cooked by slow fire 45min；Close
And decocting liquid, filtration, cures in -20 DEG C of environment, pulvis is made in frozen vacuum dryer.68 parts of Chinese medicine powder, auxiliary material 32
Part, the pulverizer of 80 mesh is all crossed, soluble powder is made.It is a packaging unit per 150g soluble powders.
Foregoing auxiliary material is powdered glucose.
Prevent usage and dosage：1-5 ages in days, the dosage of 1d is used per packaging unit for 5000 chick, and whole day is drunk, 2-3h
Replacement is once drunk water, and is faced and is added to the water with by soluble powder.9-11 ages in days, the agent of 1d is used per packaging unit for 2500 broiler chicken
Amount, with the 1-2h that before cuts off the water, concentrates 2-3h to drink, 3d is used in conjunction.
Treat usage and dosage：Per packaging unit for 500 1.5Kg weight broiler chicken 1d dosage (equivalent to being added in daily ration
0.4% traditional Chinese powder medicine).With the 1-2h that preceding cuts off the water, concentrate 2-3h to drink, 3-5d is used in conjunction.
The Chinese prescription has the effect of clearing heat and detoxicating, invigorating the spleen to arrest diarrhea, tonifying Qi and stomach, antiulcer, relieving haperacidity analgesic.
Replenish qi to invigorate the spleen in prescription, the medicine of regulating qi-flowing for harmonizing stomach function has radix astragali, Radix Codonopsis, honey-fried licorice root, rhizoma atractylodis, dried orange peel.Pass through
The use in conjunction of these Chinese medicines, achievees the purpose that to improve glandular stomach, muscular stomach function, promotes glandular stomach functional recovery, reduce gastric acid secretion.
Wherein honey-fried licorice root also has has inhibitory action to gastroxia；And play the role of antiacid and alleviate gastrointestinal smooth muscle spasm.Have
Clearing heat and detoxicating, the Chinese medicine of antibiosis and antiviral functions has：Kuh-seng, radix scutellariae, the coptis, Cortex Phellodendri, cape jasmine, Fructus Forsythiae, radix scrophulariae.It can coordinate pair
It is anti-to cause the microorganism cause of disease of broiler chicken chicken infectious provenculitis, while prevent the occurrence and development of secondary disease.The wherein coptis and Huang
Cypress also has anti-ulcer effect, contributes to the erosion of muscular stomach class cutin membrane, the healing of ulcer.There is the rhizoma anemarrhenae of clear gas purging intense heat effect and have foster
The radix rehmanniae recen of blood blood effect, process Chinese herbaceous peony, can preferably play effect of drugs.There are astringing to arrest bleeding, relieving haperacidity analgesic, hygroscopic sore
The cuttle bone of effect, on the basis of the membrane of a chicken's gizzard ulcer healing is contributed to, and honey-fried licorice root nurses one's health muscular stomach, the soda acid in glandular stomach together
Degree.
Pathogenesis and disease symptom of the invention according to broiler chicken chicken infectious provenculitis-ventriculitis, it is overall with reference to Chinese veterinarian
Idea and diagnosis and treatment theory draft Chinese prescription, then with feeding experiment Study of Traditional Chinese Medicine prescription to suffering from chicken infectious provenculitis-flesh
The broiler chicken of gastritis carries out treatment and prevention protectiveness experiment and shows that the invention can substantially reduce broiler chicken chicken infectious provenculitis-flesh
The generation of gastritis, and the meat feed ratio of broiler chicken can be significantly improved and deliver weight for sale.Control suffering from chicken infectious provenculitis-ventriculitis broiler chicken
Treat, curative effect is obvious.The soluble powder preparation technique is simple, of low cost, facilitates pharmaceutical manufacturer to produce in enormous quantities；It is soluble easily in water,
It is easy to use and safe, facilitate broiler chicken intensive manufacture to use.
Embodiment
With reference to embodiments, the present invention is described in further detail.
1 safety testing of embodiment
A kind of Chinese medicine soluble powder for preventing broiler chicken chicken infectious provenculitis-ventriculitis, it is by following weight percentage
Medicine raw material is made：Radix astragali, Radix Codonopsis, honey-fried licorice root, rhizoma anemarrhenae, kuh-seng, radix scutellariae, Cortex Phellodendri, cape jasmine, cuttle bone each 8%, rhizoma atractylodis, process Chinese herbaceous peony,
Fructus Forsythiae, radix scrophulariae, the coptis each 4.8%, dried orange peel 4%.
The preparation method of above-mentioned Chinese medicine preparation, comprises the following steps：1 part of Chinese medicine material is weighed by weight ratio, decocts two
It is secondary.Decoct for the first time：1 part of Chinese medicine, 8 parts of water, soaks 30min, boils by intense fire, be changed to be cooked by slow fire 60min, filtering liquid medicine；The
Secondary decoction：Add 6 parts of water in the first time dregs of a decoction, boil by intense fire, be changed to be cooked by slow fire 45min；Collecting decoction, filtration, -20
Cure in DEG C environment, pulvis is made in frozen vacuum dryer.68 parts of Chinese medicine powder, 32 parts of auxiliary material, whole powder for crossing 80 mesh
Broken machine, is made soluble powder.It is a packaging unit per 150g soluble powders.
Foregoing auxiliary material is powdered glucose.
1.1 acute toxicity test
18-22g Kunming small white mouse 10, half male and half female.Small white mouse fasting 12h, free water.Soluble powder is configured to
10% aqueous solution, gavages administration, each 0.4mL, and interval 2h is administered once, successive administration 3 times.24h is observed, is not found small
White mouse is dead.Total dosage 0.12g/20gBW (6000mg/kgBW).Repeat 2 times, small white mouse does not occur
It is dead.
Illustrate the soluble powder LD of the present invention50＞ 5000mg/kgBW.
1.2 subchronic toxicity test
18-22g Kunming small white mouse 80, half male and half female.Small white mouse is randomly divided into 4 groups, every group 20, male and female are each
Half.Small white mouse fasting 12h, free water.
The soluble powder of the present invention gavages small white mouse after being dissolved with distilled water.The processing method of each group small white mouse, refers to table
1。
The processing method of 1 each group small white mouse of table
The daily timing gavage processing of each group.Each group continuous gavage 14d, after drug withdrawal again continuous observation 14d.Observation mouse daily
General status, weekly measure changes of weight situation.
1.2.1 the method for subchronic toxicity test
The 1st day and the 14th day after drug withdrawal, each group randomly selects 10 blood samplings, and it is thin to measure total white blood cells, lymph immediately
Born of the same parents' number, monocyte number, hemoglobin, blood platelet, packed cell volume and Erythrocytes content.
The 1st day and 14 days after drug withdrawal, every group is randomly selected 10 blood samplings, separates serum.
Utilize the content of enzymic creatinine assay method measure creatinine；Utilize the content of reitman-frankel method measure glutamic-pyruvic transaminase；Utilize
Microplate method measures the content of glutamic-oxalacetic transaminease；Utilize the content of urease method measure urea nitrogen.
1.2.2 subchronic toxicity test result and analysis
During the experiment does not have to find that each group small white mouse has abnormal conditions.Compared with control group, experimental group I, experimental group
II and experimental group III weight slightly raise, but each group difference is not notable (P ＞ 0.05).
The determination data of each group small white mouse blood routine part index number is analyzed using statistics software.Particular content is detailed
It is shown in Table 2.
The measurement result of each group small white mouse blood routine part index number on the 1st after table 2 is discontinued
As shown in Table 2, compared with control group, total white blood cells, lymphocyte number, the monocyte of each experimental mice
Number, hemoglobin, blood platelet, packed cell volume and Erythrocytes slightly increase, but difference is not notable (P ＞ 0.05).
14 days after drug withdrawal, compared with control group, total white blood cells, lymphocyte number, the monocyte of each experimental mice
Number, hemoglobin, blood platelet, packed cell volume and Erythrocytes difference is not notable (P ＞ 0.05).
The determination data of each group mouse serum biochemical indicator is analyzed using statistics software.Particular content refers to
Table 3.
The measurement result of each group mouse serum biochemical indicator on the 1st after table 3 is discontinued
As shown in Table 3, compared with control group, each experimental mice serum alt, AST, Cr, BUN slightly increase, but poor
Different not notable (P ＞ 0.05).
14 days after drug withdrawal, compared with control group, serum alt, AST, Cr, BUN difference of each experimental mice are not shown
Write (P ＞ 0.05).
1.2.3 conclusion
During subchronic toxicity testing, observe that each experimental group small white mouse growth and development is good, and various signs are acted normally.This
The soluble powder of invention to small white mouse weight, blood glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), urea nitrogen (BUN) and
Creatinine (Cr) does not make significant difference.
1.3 long term toxicity test
1.3.1 long term toxicity test method
The soluble powder of the present invention is added in basal diet in 4% ratio, makes the soluble powder containing the present invention
Feed.
18-22g Kunming small white mouse 40, half male and half female.Small white mouse is randomly divided into 2 groups, every group 20, male and female are each
Half.The processing method of each group small white mouse, refers to table 4.
The processing method of 4 each group small white mouse of table
The daily timing feeding of each group.After experiment starts, observe daily and record the general performance, behavior, poisoning table of animal
Now and death condition.Dusk fasting in 60th day, can't help water.
Fasting 12h eye sockets take blood, separate serum, measure glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, urea nitrogen, the content of creatinine.
After mice dying, organ, the weight of measure liver, kidney and spleen are separated.
1.3.2 long term toxicity test result
During the experiment does not have to find that each group small white mouse has abnormal conditions.Compared with control group, experimental group weight differences
Not significantly (P ＞ 0.05).
The measurement result of each group mouse serum biochemical indicator refers to table 5.
The measurement result of 5 each group mouse serum biochemical indicator of table
As shown in Table 5, compared with control group, experimental mice serum alt, AST, Cr, BUN difference not significantly (P ＞
0.05)。
The measurement result of each group small white mouse organ weight refers to table 6.
The measurement result of 6 each group small white mouse organ weight of table
As shown in Table 6, compared with control group, weight differences not significantly (P of experimental mice liver, kidney and spleen
＞ 0.05).
1.3.3 long term toxicity test conclusion
During long term toxicity test, observation experiment group small white mouse growth and development is good, and various signs are acted normally.The present invention
Soluble powder to the weight of small white mouse weight, part internal organs, blood glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), urine
Plain nitrogen (BUN) and creatinine (Cr) do not make significant difference.
The conclusion of 1.4 safety testings
Pass through safety test (acute toxicity test, subchronic toxicity test and the long term toxicity of the soluble powder of the present invention
Experiment) prove, soluble powder of the invention be it is safe and reliable, can be safe to use in animal productiong.
Embodiment 2 prevents the experiment of broiler chicken chicken infectious provenculitis-ventriculitis
The Chinese medicine material of Chinese medicine soluble powder is made with preparation method with embodiment 1.
The test method of 2.1 prevention broiler chicken chicken infectious provenculitis-ventriculitis
160 broiler chicken whole process free choice feedings, 50 broiler chicken are control group, half male and half female.50 broiler chicken are prevention group, male and female
It is fifty-fifty.60 broiler chicken are treatment group, half male and half female.
Prevention group 1-5 ages in days, every 1.5 grams of soluble powders of the invention use the dosage of 1d, and whole day is drunk, and 2-3h replaces one
Secondary drinking-water, faces and is added to the water with by soluble powder.9-11 ages in days, every 3 grams of soluble powders of the invention use the dosage of 1d, before
Cut off the water 1-2h, concentrates 2-3h to drink, 3d is used in conjunction.
Prevention group and treatment group 5-9 age in days Broiler chicks start to gavage the glandular stomach supernatant 1.5mL/ of chicken infectious provenculitis broiler chicken
Only secondary, 2 times/day, feed is sprayed with 3% vinegar, micro- tide.Free choice feeding, 24h illumination.Each group Broiler chicks processing method, is referred to
Table 7.
7 each group Broiler chicks processing method of table
Note：Each stage daily ration of each group broiler chicken is certain domestic well-known same batch products of feed companies.
8 age in days feed intake of experimental group broiler chicken does not increase, and 9 ages in days are visible not of uniform size, contracting neck, beak and the decline of leg yellow,
Feed is just or fine strip shape excrement.Control group and prevention group broiler chicken are without exception.The Broiler chicks for there was only clinical symptoms to experimental group 10 cut open
Inspection, finds glandular stomach enlargement, nipple is obvious, and muscular stomach class cutin membrane thickens.Experimental group broiler chicken can be made a definite diagnosis and suffer from chicken infectious provenculitis.
The result of the test of 2.2 prevention broiler chicken chicken infectious provenculitis-ventriculitis
Counting each group has the broiler chicken of chicken infectious provenculitis clinical symptoms, obtains the incidence of each group, refers to table 8.
8 each group incidence statistical form of table
As shown in Table 8, prevention group broiler chicken does not suffer from broiler chicken and suffers from chicken infectious provenculitis.Malicious group of (experiment is attacked without prevention
Group) the broiler chicken incidence of suffering from chicken infectious provenculitis be up to 90%.
10 ages in days start to treat 50 broiler chicken of experimental group, and 5 grams of soluble powder of the invention is 50 broiler chicken 1d
Dosage.With the 1-2h that preceding cuts off the water, concentrate 2-3h to drink, 3-5d is used in conjunction.12 age in days feed intakes start to recover, and 14 age in days feed intakes are recovered
To as control group and prevention group.The clinical symptoms that broiler chicken suffers from chicken infectious provenculitis disappear.
The conclusion (of pressure testing) of 2.3 prevention broiler chicken chicken infectious provenculitis-ventriculitis
The soluble powder of the present invention is used in 1-5 ages in days, 9-11 ages in days, can prevent the hair that broiler chicken suffers from chicken infectious provenculitis
It is raw.It is used for treating after morbidity, also achieves the effect of obvious.
One embodiment of the present of invention is described in detail above, but the content is only the preferable implementation of the present invention
Example, it is impossible to be construed as limiting the practical range of the present invention.All equivalent variations made according to the present patent application scope and improvement,
It should be attributed within the covering scope of the present invention.
Claims (3)
- A kind of 1. Chinese medicine soluble powder for preventing broiler chicken chicken infectious provenculitis-ventriculitis, it is characterised in that：By Chinese medicine powder 68 Part, 32 parts of powdered glucose are made, and the Chinese medicine powder is formed by the Chinese medicine material compatibility of following weight percentage：Radix astragali, Radix Codonopsis, Honey-fried licorice root, rhizoma anemarrhenae, kuh-seng, radix scutellariae, Cortex Phellodendri, cape jasmine, cuttle bone each 8%, rhizoma atractylodis, to process Chinese herbaceous peony, Fructus Forsythiae, radix scrophulariae, the coptis each 4.8%, dried orange peel 4%.
- 2. the preparation method of Chinese medicine soluble powder described in claim 1, it is characterised in that comprise the following steps：1) match somebody with somebody by weight Than weighing 1 part of Chinese medicine material, decoct twice；2) decoct for the first time：1 part of Chinese medicine, 8 parts of water, soaks 30min, boils by intense fire, change To be cooked by slow fire 60min, filtering liquid medicine 3) second of decoction：Add 6 parts of water in the first time dregs of a decoction, boil by intense fire, be changed to slow boiling Decoct 45min；4) collecting decoction, filtration；5) cure in -20 DEG C of environment, pulvis is made in frozen vacuum dryer； 6) 68 parts of Chinese medicine powder, 32 parts of auxiliary material, all crosses the pulverizer of 80 mesh, soluble powder is made；7) it is one per 150g soluble powders A packaging unit.
- 3. the preparation method of Chinese medicine soluble powder according to claim 2, it is characterised in that：The packaging unit is vacuum Packaging, moisture-proof storage.
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| CN105395674B (en) * | 2015-11-24 | 2019-08-23 | 山东宝来利来生物工程股份有限公司 | A kind of Traditional Chinese medicine probiotic compound formulation and preparation method thereof for preventing and treating fowl gland ventriculitis |
| CN106038722A (en) * | 2016-08-11 | 2016-10-26 | 王华伟 | Traditional Chinese medicine for treating chicken proventriculitis |
| CN107184702B (en) * | 2017-07-27 | 2020-07-17 | 北京生泰尔科技股份有限公司 | Traditional Chinese medicine composition for preventing and treating proventriculitis and myogastritis of poultry and preparation method thereof |
| CN107890531A (en) * | 2017-11-17 | 2018-04-10 | 锦州景良动物药业有限公司 | A kind of traditional Chinese medicine oral liquid for treating avian infectious proventriculitis and preparation method thereof |
| CN114246940B (en) * | 2020-09-23 | 2024-07-23 | 洛阳惠中兽药有限公司 | Application of medicinal composition containing kuh-seng and mahonia powder |
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