CN105016853A - Needle mushroom medium and method using medium to cultivate needle mushroom - Google Patents
Needle mushroom medium and method using medium to cultivate needle mushroom Download PDFInfo
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- CN105016853A CN105016853A CN201510364866.5A CN201510364866A CN105016853A CN 105016853 A CN105016853 A CN 105016853A CN 201510364866 A CN201510364866 A CN 201510364866A CN 105016853 A CN105016853 A CN 105016853A
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Abstract
The invention belongs to the field of domestic fungus cultivation, and concretely relates to a needle mushroom medium and a method using the medium to cultivate needle mushroom. The needle mushroom medium comprises, by weight, 10-20 parts of tea seed shell, 10-20 parts of lotus seed shell, 10-20 parts of hazelnut shell, 20-30 parts of cottonseed shell, 10-20 parts of hemp shell, 3-5 parts of rice bran, 10-12 parts of wheat bran, 3-5 parts of wheat flour, 1-2 parts of soybean meal, 2-3 parts of tea bran, 1-3 parts of peanut bran, 1-2 parts of hemp bran,1-2 parts of cattle bone meal, 3-5 parts of quicklime powder and 1-2 parts of brown sugar. The medium has the advantages of reasonable formula and abundant nutrition, so the needle mushroom cultivated by using the medium has the advantages of plump flesh, delicious taste, good mouthfeel, high quality and fast growth.
Description
Technical field
The present invention relates to a kind of culture medium for golden mushroom and the method for cultivating flammulina velutipes thereof, belong to field of edible fungus culture.
Background technology
Needle mushroom formal name used at school hair handle money bacterium, also known as the little fiery mushroom of hair handle, structure bacterium, plain mushroom, dried mushroom, plain wild rice, freezes bacterium, golden mushroom, intelligence mushroom etc.Because its stem is elongated, like Flos Hemerocallis, therefore claim needle mushroom, belonging to Agaricales Bai Mo section pin gold mushroom and belong to, is a kind of bacterium algae lichens.Needle mushroom has very high medicinal dietary function.
Needle mushroom widely distributes at nature, and all there is distribution on the ground such as China, Japan, Russia, Europe, North America, Australia.In China North gets Heilungkiang, reach Yunnan in the south, from the east of Jiangsu, be all applicable to the growth of needle mushroom to Xinjiang.Guannan, Jiangsu, Hebei, Anyang, Linzhou City etc.Lingshou, Shijiazhuang, Hebei plants platinum needle mushroom in a large number, every 11-12 month equivalent production and marketing various places.
Needle mushroom is not containing chlorophyll, not there is photosynthesis, carbohydrate can not be manufactured, but can grow in dark surrounds completely, must absorb ready-made organic substance from substratum, as the degradation product of carbohydrate, protein and fat, be saprophytic nutrition type, be a kind of heterotroph, belong to club fungi.Needle mushroom is a kind of timber saprophytic microorganism, and easily growth is on the withered trunk and stub of the deciduous trees such as willow, elm, poplar tree.
According to surveying and determination, the amino acid whose content of needle mushroom is very abundant, and higher than general mushroom class, especially the content of Methionin is high especially, and Methionin has the function promoting children ' s intelligence development.Containing protein 8.87% in needle mushroom dry product, carbohydrate 60.2%, robust fibre reaches 7.4%, often eats and can prevent and treat Peptic Ulcers.
Research shows, a kind of material contained in needle mushroom has good antitumous effect.Needle mushroom is a kind of acat, is again good protective foods, and the domestic and international market of needle mushroom is day by day wide.Needle mushroom artificial cultivation technique is also uncomplicated, as long as can control envrionment conditions well, just easily obtains reliable and stable output.
At present, on market to the demand of needle mushroom in continuous increase, therefore, need the formula material and cultivating method of developing a kind of applicable Growth of Flammulina Velutipes badly.Contriver on affecting needle mushroom nutrition, the factor of quality and output is studied, and finds that the impact on needle mushroom nutrition, quality and output of different plantation formula materials and cultivating method is very large.
Summary of the invention
The object of the present invention is to provide a kind of culture medium for golden mushroom and the method for cultivating flammulina velutipes thereof, utilize that to the invention provides the yield of flammulina velutipes that substratum cultivates high, mouthfeel is good, quality better and being of high nutritive value.
Object of the present invention is achieved through the following technical solutions:
A kind of culture medium for golden mushroom, described substratum comprises following raw materials according by ratio of weight and the number of copies: tea seed episperm 10-20 part, lotus seed shell 10-20 part, hazelnut shell 10-20 part, cotton seed hulls 20-30 part, fiery numb shell 10-20 part, rice bran 3-5 part, wheat bran 10-12 part, wheat-flour 3-5 part, analysis for soybean powder 1-2 part, tea bran 2-3 part, peanut press pulp 1-3 part, fiery numb bran 1-2 part, bovine bone powder 1-2 part, calcium lime powder 3-5 part and brown sugar 1-2 part.
Present invention also offers the preparation method of described culture medium for golden mushroom, described preparation method comprises the following steps:
(1) tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell is crushed to the particle that particle diameter is 5-7mm;
(2) fully stirred by tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and the fire fiber crops shell after pulverizing again, and to stir with sack packer, add water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 55-65 DEG C, keeps 3d, turns over every day and push away 1 time;
(3) by rice bran, wheat bran, wheat-flour, analysis for soybean powder, tea bran, peanut press pulp, fiery numb bran, bovine bone powder, calcium lime powder and brown sugar mix and blend, and the water that can just flood the raw material put into is added, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented in step (2), continuation sack packer stirs, and continues fermentation to temperature and reaches 60 DEG C, after keeping 1d, and the substratum that must ferment.
Present invention also offers the method for described culture medium for golden mushroom for cultivating flammulina velutipes, said method comprising the steps of:
(1) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 15-18cm, length is 15-34cm;
(2) sterilizing: put into woven bag by after excellent for the cylinder bag bacterium installing substratum two doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 14-18h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(3) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is respectively put into, then doubling sack at bacterium rod two during inoculation;
(4) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25-26 DEG C, frequent ventilation, and keep air fresh, culturing room's humidity can not more than 70%;
(5) fruiting: adopt three three-dimensional mushroom springing methods to carry out fruiting;
(6) management of producing mushroom: mushroom room temperature is at 5-15 DEG C, and preferable temperature is 10 DEG C; Forced ventilation will be added simultaneously; After having the mushroom flower bud of seemingly grain of rice shape to grow, keep relative air humidity 80-90%;
(7) gather: through about 10-15 days, can gather when sporophore grows to 14-15cm height.
As preferably, the water content of described substratum is 60-65% by mass percentage.
As preferably, three concrete implementation steps of three-dimensional mushroom springing method described in step (5):
(1) mix with earth and water in mushroom canopy, stir into mud, be then built into tetrahedron with mud, the angle of each side and bottom surface is at 35-50 °, and the surface-area of each side is at 1-2 ㎡;
(2) after tetrahedron is built into, the bacterium cylinder sending out full bacterium is put toward three sides;
(3) after whole solid puzzles, be parallel to this face in each face and insert 1 pvc (Polyvinylchloride) pipe, for the fruiting later stage, whole cubical bacterium rod is built up one's health liquid.
As preferably, described in step (1), the formula of earth comprises following composition by mass percentage: 1-2% unslaked lime, 2-3% coconut palm chaff, bagasse 1-3%, 50-60% particle loam, Lentinus Edodes fungus chaff 37%, after mixing moisture 60%; It is the circular hole of 2-3cm that pvc pipe described in step (3) bores a diameter every 10cm electric drill.
As preferably, described particle loam is the earth getting thick more than 20cm soil layer from large field, and drying, is then crushed to 60-100 order.
As preferably, described Lentinus Edodes fungus chaff be gone out mushroom in cultivating champignon after lentinus edodes strain stick, namely obtain Lentinus Edodes fungus chaff after crushed.
As preferably, described in step (3), the formula of nutritive medium comprises following component by mass percentage: glucose 1-2%, potato 4-8% to boil water, unslaked lime 1-2%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water.
As preferably, described potato is to boil water filters gained for adding 1000g water after 100g potato slice after boiling.
Compared with prior art, the present invention has following beneficial effect:
1. culture medium for golden mushroom formula nutritional of the present invention is relatively more comprehensive, and the needle mushroom local flavor planted is stronger, and delicious, mouthfeel are good, Functionality, quality and appealing design.
2. make use of tea seed episperm, lotus seed shell, hazelnut shell fully in substratum of the present invention, enrich the culturing raw material of edible mushrooms.
3. adopt cultivation method of the present invention yield of flammulina velutipes out high, general culture medium raw material per jin can produce one jin of mushroom, but the needle mushroom culture medium raw material per jin of this invention cultivation can produce mushroom more than one and half.Because this invention formula nutritional is comprehensive, add that the later stage injects nutritive medium and manages, and the Ways of fruiting of three-dimensional earthing, can increase yield.
4. cultivating method of the present invention adopts the method for three three-dimensional fruitings, namely with fertile earth, the bacterium rod of fruiting is built into tetrahedral shape, has the value of sightseeing, can be developed as Eco-agricultural tourism garden.
Embodiment
Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.These embodiments only for illustration of the present invention, but not for limiting the scope of the invention.In addition, after reading content of the present invention, those skilled in the art can do various amendment to the present invention, and these equivalent variations fall within appended claims limited range of the present invention equally.
embodiment 1:
Culture medium for golden mushroom is used for a method for cultivating flammulina velutipes, and described cultivating method comprises the following steps:
(1) preparation of substratum: take following raw materials according by weight: tea seed episperm 10kg, lotus seed shell 10kg, hazelnut shell 10kg, cotton seed hulls 20kg, fiery numb shell 10kg, rice bran 3kg, wheat bran 10kg, wheat-flour 3kg, analysis for soybean powder 1kg, tea bran 2kg, peanut press pulp 1kg, fiery numb bran 1kg, bovine bone powder 1kg, calcium lime powder 3kg and brown sugar 1kg; Its preparation method comprises the following steps: first tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell is crushed to the particle that particle diameter is 5mm; Fully stirred by tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell after pulverizing, and to stir with sack packer, add water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 55 DEG C, keeps 3d, turns over every day and push away 1 time; By rice bran, wheat bran, wheat-flour, analysis for soybean powder, tea bran, peanut press pulp, fiery numb bran, bovine bone powder, calcium lime powder and brown sugar mix and blend, and add the water that can just flood the raw material put into, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation and reaches 60 DEG C to temperature, after keeping 1d, and the substratum that must ferment, the water content of described substratum is by mass percentage 60%;
(2) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 15cm, length is 15cm;
(3) sterilizing: put into woven bag by after excellent for the cylinder bag bacterium installing substratum two doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 14h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(4) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is respectively put into, then doubling sack at bacterium rod two during inoculation;
(5) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25 DEG C, frequent ventilation, and keep air fresh, culturing room's humidity can not more than 70%;
(6) fruiting: adopt three three-dimensional mushroom springing methods to carry out fruiting, concrete implementation step: with earth and water mixing in mushroom canopy, stir into mud, be then built into tetrahedron with mud, the angle of each side and bottom surface is 35 °, and the surface-area of each side is 1 ㎡; After tetrahedron is built into, the bacterium cylinder sending out full bacterium is put toward three sides; After whole solid puzzles, be parallel to this face in each face and insert 1 pvc pipe, for the fruiting later stage, whole cubical bacterium rod is built up one's health liquid; The formula of described earth comprises following component by mass percentage: 1% unslaked lime, 2% coconut palm chaff, bagasse 1%, 50% particle loam, Lentinus Edodes fungus chaff 37%, after mixing, add water by 60% of gross weight and mix thoroughly; It is the circular hole of 2cm that pvc pipe described in step (3) bores a diameter every 10cm electric drill; Described particle loam is the earth getting thick more than 20cm soil layer from large field, and drying, is then crushed to 60 orders; Described Lentinus Edodes fungus chaff be gone out mushroom in cultivating champignon after lentinus edodes strain stick, namely obtain Lentinus Edodes fungus chaff after crushed; The formula of described nutritive medium comprises following component by mass percentage: glucose 1%, potato are to boil water 4%, unslaked lime 1%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water; Described potato is to boil water filters gained for adding 1000g water after 100g potato slice after boiling;
(7) management of producing mushroom: mushroom room temperature is at 5 DEG C; Forced ventilation will be added simultaneously; After having the mushroom flower bud of seemingly grain of rice shape to grow, keep relative air humidity 80%;
(8) gather: through about 10 days, can gather when sporophore grows to 14cm height.
embodiment 2:
Culture medium for golden mushroom is used for a method for cultivating flammulina velutipes, and described cultivating method comprises the following steps:
(1) preparation of substratum: take following raw materials according by weight: tea seed episperm 20kg, lotus seed shell 20kg, hazelnut shell 20kg, cotton seed hulls 30kg, fiery numb shell 20kg, rice bran 5kg, wheat bran 12kg, wheat-flour 5kg, analysis for soybean powder 2kg, tea bran 3kg, peanut press pulp 3kg, fiery numb bran 2kg, bovine bone powder 2kg, calcium lime powder 5kg and brown sugar 2kg; Its preparation method comprises the following steps: first tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell is crushed to the particle that particle diameter is 7mm; Fully stirred by tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell after pulverizing, and to stir with sack packer, add water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 65 DEG C, keeps 3d, turns over every day and push away 1 time; By rice bran, wheat bran, wheat-flour, analysis for soybean powder, tea bran, peanut press pulp, fiery numb bran, bovine bone powder, calcium lime powder and brown sugar mix and blend, and add the water that can just flood the raw material put into, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation and reaches 60 DEG C to temperature, after keeping 1d, and the substratum that must ferment, the water content of described substratum is by mass percentage 65%;
(2) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 18cm, length is 34cm;
(3) sterilizing: put into woven bag by after excellent for the cylinder bag bacterium installing substratum two doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 18h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(4) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is respectively put into, then doubling sack at bacterium rod two during inoculation;
(5) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 26 DEG C, frequent ventilation, and keep air fresh, culturing room's humidity can not more than 70%;
(6) fruiting: adopt three three-dimensional mushroom springing methods to carry out fruiting, concrete implementation step: with earth and water mixing in mushroom canopy, stir into mud, be then built into tetrahedron with mud, the angle of each side and bottom surface is 50 °, and the surface-area of each side is 2 ㎡; After tetrahedron is built into, the bacterium cylinder sending out full bacterium is put toward three sides; After whole solid puzzles, be parallel to this face in each face and insert 1 pvc pipe, for the fruiting later stage, whole cubical bacterium rod is built up one's health liquid; The formula of described earth comprises following component by mass percentage: 2% unslaked lime, 3% coconut palm chaff, bagasse 3%, 60% particle loam, Lentinus Edodes fungus chaff 37%, after mixing, add water by 60% of gross weight and mix thoroughly; It is the circular hole of 3cm that pvc pipe described in step (3) bores a diameter every 10cm electric drill; Described particle loam is the earth getting thick more than 20cm soil layer from large field, and drying, is then crushed to 100 orders; Described Lentinus Edodes fungus chaff be gone out mushroom in cultivating champignon after lentinus edodes strain stick, namely obtain Lentinus Edodes fungus chaff after crushed; The formula of described nutritive medium comprises following component by mass percentage: glucose 2%, potato are to boil water 8%, unslaked lime 2%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water; Described potato is to boil water filters gained for adding 1000g water after 100g potato slice after boiling;
(7) management of producing mushroom: mushroom room temperature is at 15 DEG C; Forced ventilation will be added simultaneously; After having the mushroom flower bud of seemingly grain of rice shape to grow, keep relative air humidity 90%;
(8) gather: through about 15 days, can gather when sporophore grows to 15cm height.
embodiment 3:
Culture medium for golden mushroom is used for a method for cultivating flammulina velutipes, and described cultivating method comprises the following steps:
(1) preparation of substratum: take following raw materials according by weight: tea seed episperm 15kg, lotus seed shell 15kg, hazelnut shell 15kg, cotton seed hulls 25kg, fiery numb shell 15kg, rice bran 4kg, wheat bran 11kg, wheat-flour 4kg, analysis for soybean powder 1.5kg, tea bran 2.5kg, peanut press pulp 2kg, fiery numb bran 1.5kg, bovine bone powder 1.5kg, calcium lime powder 4kg and brown sugar 1.5kg; Its preparation method comprises the following steps: first tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell is crushed to the particle that particle diameter is 6mm; Fully stirred by tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell after pulverizing, and to stir with sack packer, add water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 60 DEG C, keeps 3d, turns over every day and push away 1 time; By rice bran, wheat bran, wheat-flour, analysis for soybean powder, tea bran, peanut press pulp, fiery numb bran, bovine bone powder, calcium lime powder and brown sugar mix and blend, and add the water that can just flood the raw material put into, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation and reaches 60 DEG C to temperature, after keeping 1d, and the substratum that must ferment, the water content of described substratum is by mass percentage 63%;
(2) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 17cm, length is 25cm;
(3) sterilizing: put into woven bag by after excellent for the cylinder bag bacterium installing substratum two doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 16h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(4) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is respectively put into, then doubling sack at bacterium rod two during inoculation;
(5) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25.5 DEG C, frequent ventilation, and keep air fresh, culturing room's humidity can not more than 70%;
(6) fruiting: adopt three three-dimensional mushroom springing methods to carry out fruiting, concrete implementation step: with earth and water mixing in mushroom canopy, stir into mud, be then built into tetrahedron with mud, the angle of each side and bottom surface is 45 °, and the surface-area of each side is 4.5 ㎡; After tetrahedron is built into, the bacterium cylinder sending out full bacterium is put toward three sides; After whole solid puzzles, be parallel to this face in each face and insert 1 pvc pipe, for the fruiting later stage, whole cubical bacterium rod is built up one's health liquid; The formula of described earth comprises following component by mass percentage: 1.5% unslaked lime, 2.5% coconut palm chaff, bagasse 2%, 55% particle loam, Lentinus Edodes fungus chaff 37%, after mixing, add water by 60% of gross weight and mix thoroughly; It is the circular hole of 2.5cm that pvc pipe described in step (3) bores a diameter every 10cm electric drill; Described particle loam is the earth getting thick more than 20cm soil layer from large field, and drying, is then crushed to 80 orders; Described Lentinus Edodes fungus chaff be gone out mushroom in cultivating champignon after lentinus edodes strain stick, namely obtain Lentinus Edodes fungus chaff after crushed; The formula of described nutritive medium comprises following component by mass percentage: glucose 1.5%, potato are to boil water 6%, unslaked lime 1.5%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water; Described potato is to boil water filters gained for adding 1000g water after 100g potato slice after boiling;
(7) management of producing mushroom: mushroom room temperature is at 10 DEG C; Forced ventilation will be added simultaneously; After having the mushroom flower bud of seemingly grain of rice shape to grow, keep relative air humidity 85%;
(8) gather: through about 12 days, can gather when sporophore grows to 14.5cm height.
Good, the Functionality, quality and appealing design of method cultivation gained needle mushroom delicious, mouthfeel in above-described embodiment 1-3; High through cultivation method of the present invention yield of flammulina velutipes out, culture medium raw material per jin can produce needle mushroom more than one and half; Cultivating method of the present invention adopts the method for three-dimensional five fruitings, namely with fertile earth, the bacterium rod of fruiting is built into cubical shape, has the value of sightseeing, can be developed as Eco-agricultural tourism garden.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (10)
1. a culture medium for golden mushroom, it is characterized in that, described substratum comprises following raw materials according by ratio of weight and the number of copies: tea seed episperm 10-20 part, lotus seed shell 10-20 part, hazelnut shell 10-20 part, cotton seed hulls 20-30 part, fiery numb shell 10-20 part, rice bran 3-5 part, wheat bran 10-12 part, wheat-flour 3-5 part, analysis for soybean powder 1-2 part, tea bran 2-3 part, peanut press pulp 1-3 part, fiery numb bran 1-2 part, bovine bone powder 1-2 part, calcium lime powder 3-5 part and brown sugar 1-2 part.
2. the preparation method of culture medium for golden mushroom according to claim 1, is characterized in that, described preparation method comprises the following steps:
(1) tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and fire fiber crops shell is crushed to the particle that particle diameter is 5-7mm;
(2) fully stirred by tea seed episperm, lotus seed shell, hazelnut shell, cotton seed hulls and the fire fiber crops shell after pulverizing again, and to stir with sack packer, add water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 55-65 DEG C, keeps 3d, turns over every day and push away 1 time;
(3) by rice bran, wheat bran, wheat-flour, analysis for soybean powder, tea bran, peanut press pulp, fiery numb bran, bovine bone powder, calcium lime powder and brown sugar mix and blend, and the water that can just flood the raw material put into is added, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented in step (2), continuation sack packer stirs, and continues fermentation to temperature and reaches 60 DEG C, after keeping 1d, and the substratum that must ferment.
3. culture medium for golden mushroom according to claim 1 is used for the method for cultivating flammulina velutipes, and it is characterized in that, described cultivating method comprises the following steps:
(1) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 15-18cm, length is 15-34cm;
(2) sterilizing: put into woven bag by after excellent for the cylinder bag bacterium installing substratum two doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 14-18h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(3) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is respectively put into, then doubling sack at bacterium rod two during inoculation;
(4) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25-26 DEG C, frequent ventilation, and keep air fresh, culturing room's humidity can not more than 70%;
(5) fruiting: adopt three three-dimensional mushroom springing methods to carry out fruiting;
(6) management of producing mushroom: mushroom room temperature is at 5-15 DEG C, and preferable temperature is 10 DEG C; Forced ventilation will be added simultaneously; After having the mushroom flower bud of seemingly grain of rice shape to grow, keep relative air humidity 80-90%;
(7) gather: through about 10-15 days, can gather when sporophore grows to 14-15cm height.
4. method according to claim 3, is characterized in that, the water content of described substratum is 60-65% by mass percentage.
5. method according to claim 3, is characterized in that, three concrete implementation steps of three-dimensional mushroom springing method described in step (5):
(1) mix with earth and water in mushroom canopy, stir into mud, be then built into tetrahedron with mud, the angle of each side and bottom surface is 35-50 °, and the surface-area of each side is 1-2 ㎡;
(2) after tetrahedron is built into, the bacterium cylinder sending out full bacterium is put toward three sides;
(3) after whole solid puzzles, be parallel to this face in each face and insert 1 pvc pipe, for the fruiting later stage, whole cubical bacterium rod is built up one's health liquid.
6. method according to claim 5, it is characterized in that, described in step (1), the formula of earth comprises following component by mass percentage: 1-2% unslaked lime, 2-3% coconut palm chaff, bagasse 1-3%, 50-60% particle loam, Lentinus Edodes fungus chaff 37%, after mixing, add water by 60% of gross weight and mix thoroughly; It is the circular hole of 2-3cm that pvc pipe described in step (3) bores a diameter every 10cm electric drill.
7. cultivating method according to claim 6, it is characterized in that, described particle loam is the earth getting thick more than 20cm soil layer from large field, and drying, is then crushed to 60-100 order.
8. method according to claim 6, is characterized in that, described Lentinus Edodes fungus chaff be gone out mushroom in cultivating champignon after lentinus edodes strain stick, namely obtain Lentinus Edodes fungus chaff after crushed.
9. method according to claim 5, it is characterized in that, described in step (3), the formula of nutritive medium comprises following component by mass percentage: glucose 1-2%, potato 4-8% to boil water, unslaked lime 1-2%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water.
10. method according to claim 9, is characterized in that, described potato is to boil water filters gained for adding 1000g water after 100g potato slice after boiling.
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| CN106220267A (en) * | 2016-07-11 | 2016-12-14 | 赖卫华 | Flammulina velutiper (Fr.) Sing special culture material |
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| CN111527990A (en) * | 2020-04-14 | 2020-08-14 | 江西省蚕桑茶叶研究所(江西省经济作物研究所) | Mushroom substitute cultivation medium and preparation method thereof |
| CN111527990B (en) * | 2020-04-14 | 2022-04-15 | 江西省蚕桑茶叶研究所(江西省经济作物研究所) | Mushroom substitute cultivation medium and preparation method thereof |
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