CN105016814A - Agaricus bisporus medium and method using medium to cultivate Agaricus bisporus - Google Patents

Agaricus bisporus medium and method using medium to cultivate Agaricus bisporus Download PDF

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Publication number
CN105016814A
CN105016814A CN201510366627.3A CN201510366627A CN105016814A CN 105016814 A CN105016814 A CN 105016814A CN 201510366627 A CN201510366627 A CN 201510366627A CN 105016814 A CN105016814 A CN 105016814A
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bacterium
parts
mushroom
substratum
bran
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CN201510366627.3A
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何金霞
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何金霞
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention belongs to the field of domestic fungus cultivation, and concretely relates to a Agaricus bisporus medium and a method using the medium to cultivate Agaricus bisporus. The Agaricus bisporus medium comprises, by weight, 10-15 parts of tea seed shell, 10-15 parts of lotus seed shell, 10-15 parts of peanut shell, 10-15 parts of hemp shell, 5-10 parts of chicken manure, 5-10 parts of sheep manure, 10-30 parts of wheat straws, 8-10 parts of wheat bran, 6-10 parts of corn flour, 2-4 parts of tea bran, 1-2 parts of hemp bran, 1-2 parts of cattle bone meal, 3-4 parts of lime powder and 1-2 parts of brown sugar. The medium has the advantages of reasonable formula and abundant nutrition, so the Agaricus bisporus cultivated by using the medium has the advantages of plump flesh, delicious taste, good mouthfeel, high quality and fast growth.

Description

A kind of bisporous mushroom substratum and the method for cultivating agaricus bisporus thereof
Technical field
The present invention relates to a kind of bisporous mushroom substratum and the method for cultivating agaricus bisporus thereof, belong to field of edible fungus culture.
Background technology
Bisporous mushroom, mycelia silvery white, fast in the speed of growth, not easily tie bacterium quilt, the many Dan Sheng of sporophore, circle just, white, without scale, cap is thick, not easily parachute-opening, and thick shorter in stem, bacterial context white, organizes solid, stem has half membranaceous collarium, spore silver brown.One of modal edible bacterium, meat is plump.Primary in Europe and North America, the mankind at least just start to eat from ancient Greek's epoch, and artificial culture then about starts from the ground such as the France of the 17th century, now then extensively cultivate all over the world.
Bisporous mushroom protein content is 35-38%, containing 6 seed amino acids that human body is necessary, also containing abundant VITMAIN B1, Lin Suanna Vitamin B2 Sodium Phosphate, vitamin PP, Nucleotide, nicotinic acid, xitix and vitamins D etc., its nutritive value is 4-12 times of vegetables and fruit, enjoys " protective foods " and " king in element " laudatory title.Dark in domestic market, the especially favor of world market.
Lentinan contained by bisporous mushroom and M-band have certain antitumour activity, can the generation of Tumor suppression; Contained tyrosine oxidase can dissolve certain cholesterol, to the certain effect that reduced blood pressure; Contained trypsinase, barley-sugar enzyme etc. all contribute to the digestion of food.The traditional Chinese medical science thinks that the sweet property of bisporous mushroom taste puts down digestion of refreshing oneself, hypotensive effect.Often edible bisporous mushroom, can prevent vitamin C deficiency, prevention cancer, promotes that deleading is conciliate in wound healing, arsenic, mercury etc. poisoning, has tonifying spleen concurrently, moistening lung, regulates the flow of vital energy, clears phlegm, can prevent pernicious anemia, improve neural function, reduce blood fat.
Bisporous mushroom is not only a kind of delicious flavour, the mushroom class vegetables that nutrition is complete, and is heath food with health role.
At present, on market to the demand of bisporous mushroom in continuous increase, therefore, need formula material and the cultivating method of a kind of applicable bisporous mushroom growth of exploitation badly.Contriver on affecting bisporous mushroom nutrition, the factor of quality and output is studied, and finds that the impact on bisporous mushroom nutrition, quality and output of different plantation formula materials and cultivating method is very large.
Summary of the invention
The object of the present invention is to provide a kind of bisporous mushroom substratum and the method for cultivating agaricus bisporus thereof, utilize that to the invention provides the bisporous mushroom output that substratum cultivates high, mouthfeel is good, quality better and being of high nutritive value.
Object of the present invention is achieved through the following technical solutions:
A kind of bisporous mushroom substratum, described substratum comprises following raw material by ratio of weight and the number of copies: tea seed episperm 10-15 part, lotus seed shell 10-15 part, Pericarppium arachidis hypogaeae 10-15 part, fiery numb shell 10-15 part, chicken manure 5-10 part, sheep excrement 5-10 part, wheat-straw 10-30 part, wheat bran 8-10 part, Semen Maydis powder 6-10 part, tea bran 2-4 part, fiery numb bran 1-2 part, bovine bone powder 1-2 part, lime powder 3-4 part and brown sugar 1-2 part.
Present invention also offers the preparation method of described bisporous mushroom substratum, described preparation method comprises the following steps:
(1) tea seed episperm, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, mesh wheat straw powder are broken to the particle that particle diameter is 5-7mm;
(2) tea seed episperm after pulverizing, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, wheat straw and chicken manure, sheep excrement are fully stirred, and stirred with sack packer, add water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 55-65 DEG C, keeps 3d, turns over every day and push away 1 time;
(3) by wheat bran, Semen Maydis powder, tea bran, fiery numb bran, bovine bone powder, lime powder and brown sugar mix and blend, and the water that can just flood the raw material put into is added, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation to temperature and reaches 60 DEG C, after keeping 1d, and the substratum that must ferment.
Present invention also offers described bisporous mushroom substratum for the method for cultivating agaricus bisporus, said method comprising the steps of:
(1) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 15-18cm, length is 25-35cm, sack only has one end open;
(2) sterilizing: put into woven bag by after excellent for the bacterium installing substratum one end doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 13-15h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(3) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is put into, then doubling sack in bacterium rod one end during inoculation;
(4) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25-26 DEG C, frequent ventilation, and culturing room's humidity can not more than 70%;
(5) fruiting: adopt three-dimensional turriform mushroom springing method to carry out fruiting;
(6) manage after fruiting: in maintenance culturing room, atmospheric moisture is at 80-90%; After fruiting, temperature is at 15-20 DEG C; In conjunction with stronger ventilation amount, daytime closes, night ventilation; After a collection of mushroom of gathering, need to bacterium cylinder Additional nutrient solution;
(7) gather: gather in batches, with right hand thumb, forefinger and middle finger, mushroom should be rotated gently during harvesting, bacterium bright and lower floor mycelium is departed from, then picks up with finger, cut the mushroom handle of band mud.
As preferably, the water content of described substratum is 60-65% by mass percentage.
As preferably, described in step (5), three-dimensional turriform mushroom springing method comprises following implementation step:
(1) after the mycelia of bacterium cylinder is all covered with, bacterium cylinder can be moved on to fruiting field, the thick 3-4cm of earthing;
(2), after treating that bacterium cylinder earthing is complete, built into the cylinder of diameter 100-200cm, put bacterium cylinder according to the order from skin to internal layer successively; First at outermost layer pendulum two circle bacterium cylinder, remaining empty part covers in, and height is the same with bacterium cylinder; Then put the 2nd layer, directly putting on the earth of the 1st layer for the 2nd layer, is also put two circles, and empty part covers in; Also analogize for 3rd layer, straight until put layer 4-7, height 1-1.8m;
(3) fruiting indoor air relative humidity is controlled at 80-85%, suitable ventilation.
As preferably, the soil that described in step (1), earthing is used is for getting the soil apart from earth's surface below 20cm; The soil fetched dries, and is crushed to thin soya bean size, first with 1% lime aqueous solution adjustment grogs moisture before earthing, makes grogs water content at 25-35%.
As preferably, described in step (6), nutrient solution prescription comprises following component by mass percentage: brown sugar 1%, and potato is to boil water 5%, lime 3%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water.
As preferably, described potato is to boil water is add 1000 grams of water after 100 grams of potato slices to filter gained after boiling.
Compared with prior art, the present invention has following beneficial effect:
1. bisporous mushroom culture medium prescription of the present invention nutrition is relatively more comprehensive, and the bisporous mushroom local flavor planted is stronger, and delicious, mouthfeel are good, Functionality, quality and appealing design.
2. make use of tea seed episperm, lotus seed shell, Pericarppium arachidis hypogaeae, chicken manure, sheep excrement in substratum of the present invention fully, enrich the culturing raw material of edible mushrooms.
3. adopt cultivation method of the present invention bisporous mushroom output out high, general raw material per jin can produce 1 jin of mushroom, but the bisporous mushroom culture medium raw material per jin of this invention cultivation can produce more than 1.3 jin, mushroom.Because this invention formula nutritional is comprehensive, add that the later stage injects nutritive medium and manages, and the Ways of fruiting of three-dimensional earthing, can increase yield.
4. cultivating method of the present invention adopts the method for three-dimensional turriform fruiting, has the value of sightseeing, can be developed as Eco-agricultural tourism garden.
Embodiment
Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.These embodiments only for illustration of the present invention, but not for limiting the scope of the invention.In addition, after reading content of the present invention, those skilled in the art can do various amendment to the present invention, and these equivalent variations fall within appended claims limited range of the present invention equally.
embodiment 1:
Bisporous mushroom substratum is used for a method for cultivating agaricus bisporus, and described cultivating method comprises the following steps:
(1) preparation of substratum: take following raw material by weight: tea seed episperm 10kg, lotus seed shell 10kg, Pericarppium arachidis hypogaeae 10kg, fiery numb shell 10kg, chicken manure 5kg, sheep excrement 5kg, wheat-straw 10kg, wheat bran 8kg, Semen Maydis powder 6kg, tea bran 2kg, fiery numb bran 1kg, bovine bone powder 1kg, lime powder 3kg and brown sugar 1kg; Its preparation method comprises the following steps: tea seed episperm, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, mesh wheat straw powder are broken to the particle that particle diameter is 5mm; Tea seed episperm after pulverizing, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, wheat straw and chicken manure, sheep excrement are fully stirred, and stirred with sack packer, adds water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 55 DEG C, keeps 3d, turns over every day and push away 1 time; By wheat bran, Semen Maydis powder, tea bran, fiery numb bran, bovine bone powder, lime powder and brown sugar mix and blend, and add the water that can just flood the raw material put into, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation and reaches 60 DEG C to temperature, after keeping 1d, and the substratum that must ferment, the water content of described substratum is by mass percentage 60%;
(2) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 15cm, length is 25cm, sack only has one end open;
(3) sterilizing: put into woven bag by after excellent for the bacterium installing substratum one end doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 13h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(4) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is put into, then doubling sack in bacterium rod one end during inoculation;
(5) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25 DEG C, frequent ventilation, and culturing room's humidity can not more than 70%;
(6) fruiting: adopt three-dimensional turriform mushroom springing method to carry out fruiting, comprise following implementation step: the mycelia of bacterium cylinder can move on to fruiting field bacterium cylinder, the thick 3cm of earthing after all covering with; After treating that bacterium cylinder earthing is complete, built into the cylinder of diameter 100cm, put bacterium cylinder according to the order from skin to internal layer successively; First at outermost layer pendulum two circle bacterium cylinder, remaining empty part covers in, and height is the same with bacterium cylinder; Then put the 2nd layer, directly putting on the earth of the 1st layer for the 2nd layer, is also put two circles, and empty part covers in; Also analogize for 3rd layer, until put the 4th layer, height 1m; Control fruiting indoor air relative humidity 80%, suitable ventilation; Described earthing soil used is for getting the soil apart from earth's surface below 20cm; The soil fetched dries, and is crushed to thin soya bean size, first with 1% lime aqueous solution adjustment grogs moisture before earthing, makes grogs water content 25%; Described nutrient solution prescription comprises following component by mass percentage: brown sugar 1%, and potato is to boil water 5%, lime 3%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water; Described potato is to boil water filters gained for 100g potato slice is added 1000g water after boiling;
(7) manage after fruiting: in maintenance culturing room, atmospheric moisture is 80%; After fruiting, temperature is at 15 DEG C; In conjunction with stronger ventilation amount, daytime closes, night ventilation; After a collection of mushroom of gathering, need to bacterium cylinder Additional nutrient solution;
(8) gather: gather in batches, with right hand thumb, forefinger and middle finger, mushroom should be rotated gently during harvesting, bacterium bright and lower floor mycelium is departed from, then picks up with finger, cut the mushroom handle of band mud.
embodiment 2:
Bisporous mushroom substratum is used for a method for cultivating agaricus bisporus, and described cultivating method comprises the following steps:
(1) preparation of substratum: take following raw material by weight: tea seed episperm 15kg, lotus seed shell 15kg, Pericarppium arachidis hypogaeae 15kg, fiery numb shell 15kg, chicken manure 10kg, sheep excrement 10kg, wheat-straw 30kg, wheat bran 10kg, Semen Maydis powder 10kg, tea bran 4kg, fiery numb bran 2kg, bovine bone powder 2kg, lime powder 4kg and brown sugar 2kg; Its preparation method comprises the following steps: tea seed episperm, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, mesh wheat straw powder are broken to the particle that particle diameter is 7mm; Tea seed episperm after pulverizing, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, wheat straw and chicken manure, sheep excrement are fully stirred, and stirred with sack packer, adds water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 65 DEG C, keeps 3d, turns over every day and push away 1 time; By wheat bran, Semen Maydis powder, tea bran, fiery numb bran, bovine bone powder, lime powder and brown sugar mix and blend, and add the water that can just flood the raw material put into, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation and reaches 60 DEG C to temperature, after keeping 1d, and the substratum that must ferment, the water content of described substratum is by mass percentage 65%;
(2) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 18cm, length is 35cm, sack only has one end to be opening;
(3) sterilizing: put into woven bag by after excellent for the bacterium installing substratum one end doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 15h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(4) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is put into, then doubling sack in bacterium rod one end during inoculation;
(5) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 26 DEG C, frequent ventilation, and culturing room's humidity can not more than 70%;
(6) fruiting: adopt three-dimensional turriform mushroom springing method to carry out fruiting, comprise following implementation step: the mycelia of bacterium cylinder can move on to fruiting field bacterium cylinder, the thick 4cm of earthing after all covering with; After treating that bacterium cylinder earthing is complete, built into the cylinder of diameter 200cm, put bacterium cylinder according to the order from skin to internal layer successively; First at outermost layer pendulum two circle bacterium cylinder, remaining empty part covers in, and height is the same with bacterium cylinder; Then put the 2nd layer, directly putting on the earth of the 1st layer for the 2nd layer, is also put two circles, and empty part covers in; Also analogize for 3rd layer, until put the 7th layer, height 1.8m; Control fruiting indoor air relative humidity 85%, suitable ventilation; Described earthing soil used is for getting the soil apart from earth's surface below 20cm; The soil fetched dries, and is crushed to thin soya bean size, first with 1% lime aqueous solution adjustment grogs moisture before earthing, makes grogs water content 35%; Described nutrient solution prescription comprises following component by mass percentage: brown sugar 1%, and potato is to boil water 5%, lime 3%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water; Described potato is to boil water filters gained for 100g potato slice is added 1000g water after boiling;
(7) manage after fruiting: in maintenance culturing room, atmospheric moisture is 90%; After fruiting, temperature is at 20 DEG C; In conjunction with stronger ventilation amount, daytime closes, night ventilation; After a collection of mushroom of gathering, need to bacterium cylinder Additional nutrient solution;
(8) gather: gather in batches, with right hand thumb, forefinger and middle finger, mushroom should be rotated gently during harvesting, bacterium bright and lower floor mycelium is departed from, then picks up with finger, cut the mushroom handle of band mud.
embodiment 3:
Bisporous mushroom substratum is used for a method for cultivating agaricus bisporus, and described cultivating method comprises the following steps:
(1) preparation of substratum: take following raw material by weight: tea seed episperm 12kg, lotus seed shell 13kg, Pericarppium arachidis hypogaeae 12kg, fiery numb shell 13kg, chicken manure 8kg, sheep excrement 8kg, wheat-straw 20kg, wheat bran 9kg, corn 8kg, tea bran 3kg, fiery numb bran 1.5kg, bovine bone powder 1.5kg, lime powder 3.5kg and brown sugar 1.5kg; Its preparation method comprises the following steps: tea seed episperm, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, mesh wheat straw powder are broken to the particle that particle diameter is 6mm; Tea seed episperm after pulverizing, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, wheat straw and chicken manure, sheep excrement are fully stirred, and stirred with sack packer, adds water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 60 DEG C, keeps 3d, turns over every day and push away 1 time; By wheat bran, Semen Maydis powder, tea bran, fiery numb bran, bovine bone powder, lime powder and brown sugar mix and blend, and add the water that can just flood the raw material put into, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation and reaches 60 DEG C to temperature, after keeping 1d, and the substratum that must ferment, the water content of described substratum is by mass percentage 63%;
(2) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 16cm, length is 30cm, sack only has one end to be opening;
(3) sterilizing: put into woven bag by after excellent for the bacterium installing substratum one end doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 14h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(4) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is put into, then doubling sack in bacterium rod one end during inoculation;
(5) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25.5 DEG C, frequent ventilation, and culturing room's humidity can not more than 70%;
(6) fruiting: adopt three-dimensional turriform mushroom springing method to carry out fruiting, comprise following implementation step: the mycelia of bacterium cylinder can move on to fruiting field bacterium cylinder, the thick 3.5cm of earthing after all covering with; After treating that bacterium cylinder earthing is complete, built into the cylinder of diameter 150cm, put bacterium cylinder according to the order from skin to internal layer successively; First at outermost layer pendulum two circle bacterium cylinder, remaining empty part covers in, and height is the same with bacterium cylinder; Then put the 2nd layer, directly putting on the earth of the 1st layer for the 2nd layer, is also put two circles, and empty part covers in; Also analogize for 3rd layer, until put the 6th layer, height 1.5m; Control fruiting indoor air relative humidity 82%, suitable ventilation; Described earthing soil used is for getting the soil apart from earth's surface below 20cm; The soil fetched dries, and is crushed to thin soya bean size, first with 1% lime aqueous solution adjustment grogs moisture before earthing, makes grogs water content 30%; Described nutrient solution prescription comprises following component by mass percentage: brown sugar 1%, and potato is to boil water 5%, lime 3%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water; Described potato is to boil water filters gained for 100g potato slice is added 1000g water after boiling;
(7) manage after fruiting: in maintenance culturing room, atmospheric moisture is 85%; After fruiting, temperature is at 18 DEG C; In conjunction with stronger ventilation amount, daytime closes, night ventilation; After a collection of mushroom of gathering, need to bacterium cylinder Additional nutrient solution;
(8) gather: gather in batches, with right hand thumb, forefinger and middle finger, mushroom should be rotated gently during harvesting, bacterium bright and lower floor mycelium is departed from, then picks up with finger, cut the mushroom handle of band mud.
Good, the Functionality, quality and appealing design of method cultivation gained bisporous mushroom delicious, mouthfeel in above-described embodiment 1-3; High through cultivation method of the present invention bisporous mushroom output out, substratum per jin can produce bisporous mushroom more than one and half jin; Cultivating method of the present invention adopts the method for three-dimensional turriform fruiting, has the value of sightseeing, can be developed as Eco-agricultural tourism garden.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.

Claims (8)

1. a bisporous mushroom substratum, it is characterized in that, described substratum comprises following raw material by ratio of weight and the number of copies: tea seed episperm 10-15 part, lotus seed shell 10-15 part, Pericarppium arachidis hypogaeae 10-15 part, fiery numb shell 10-15 part, chicken manure 5-10 part, sheep excrement 5-10 part, wheat-straw 10-30 part, wheat bran 8-10 part, Semen Maydis powder 6-10 part, tea bran 2-4 part, fiery numb bran 1-2 part, bovine bone powder 1-2 part, lime powder 3-4 part and brown sugar 1-2 part.
2. the preparation method of bisporous mushroom substratum according to claim 1, is characterized in that, described preparation method comprises the following steps:
(1) tea seed episperm, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, mesh wheat straw powder are broken to the particle that particle diameter is 5-7mm;
(2) tea seed episperm after pulverizing, lotus seed shell, Pericarppium arachidis hypogaeae, fiery numb shell, wheat straw and chicken manure, sheep excrement are fully stirred, and stirred with sack packer, add water while stirring, humidity can have water to ooze out but not ooze be advisable with hand-tight culture material of holding; By the material heap fermentation mixed; Leavening temperature is 55-65 DEG C, keeps 3d, turns over every day and push away 1 time;
(3) by wheat bran, Semen Maydis powder, tea bran, fiery numb bran, bovine bone powder, lime powder and brown sugar mix and blend, and the water that can just flood the raw material put into is added, after stirring, in the pasty state; Place 4h, allow these culture materials fully mix; Then pour in the culture material fermented, continuation sack packer stirs, and continues fermentation to temperature and reaches 60 DEG C, after keeping 1d, and the substratum that must ferment.
3. bisporous mushroom substratum according to claim 1 is used for the method for cultivating agaricus bisporus, and it is characterized in that, described cultivating method comprises the following steps:
(1) pack: the substratum sack packer fermented is packed; The film cylinder bag that sack is diameter is 15-18cm, length is 25-35cm, sack only has one end open;
(2) sterilizing: put into woven bag by after excellent for the bacterium installing substratum one end doubling, and then put into Autoclave; When sterilising temp reaches 100 DEG C, after keeping 13-15h, stop heating, after keeping 12h, take out the bacterium rod after sterilizing;
(3) inoculate: bacterium rod good for sterilizing is put into transfer room, light guarantor mushroom king and aerosol sterilization is carried out to bacterium rod, after to be sterilized, carry out spraying with 84 thimerosals to transfer room before taking out the inoculation of bacterium rod again to purify air, the thick bacterial classification of one deck 3cm is put into, then doubling sack in bacterium rod one end during inoculation;
(4) send out bacterium to cultivate: the culturing room that dark surrounds put into by postvaccinal bacterium rod cultivated, room temp controls at 25-26 DEG C, frequent ventilation, and culturing room's humidity can not more than 70%;
(5) fruiting: adopt three-dimensional turriform mushroom springing method to carry out fruiting;
(6) manage after fruiting: in maintenance culturing room, atmospheric moisture is at 80-90%; After fruiting, temperature is at 15-20 DEG C; In conjunction with stronger ventilation amount, daytime closes, night ventilation; After a collection of mushroom of gathering, need to bacterium cylinder Additional nutrient solution;
(7) gather: gather in batches, with right hand thumb, forefinger and middle finger, mushroom should be rotated gently during harvesting, bacterium bright and lower floor mycelium is departed from, then picks up with finger, cut the mushroom handle of band mud.
4. method according to claim 3, is characterized in that, the water content of described substratum is 60-65% by mass percentage.
5. method according to claim 3, is characterized in that, described in step (5), three-dimensional turriform mushroom springing method comprises following implementation step:
(1) after the mycelia of bacterium cylinder is all covered with, bacterium cylinder can be moved on to fruiting field, the thick 3-4cm of earthing;
(2), after treating that bacterium cylinder earthing is complete, built into the cylinder of diameter 100-200cm, put bacterium cylinder according to the order from skin to internal layer successively; First at outermost layer pendulum two circle bacterium cylinder, remaining empty part covers in, and height is the same with bacterium cylinder; Then put the 2nd layer, directly putting on the earth of the 1st layer for the 2nd layer, is also put two circles, and empty part covers in; Also analogize for 3rd layer, until put layer 4-7, height 1-1.8m;
(3) fruiting indoor air relative humidity is controlled at 80-85%, suitable ventilation.
6. method according to claim 5, is characterized in that, the soil that described in step (1), earthing is used is for getting the soil apart from earth's surface below 20cm; The soil fetched dries, and is crushed to thin soya bean size, first with 1% lime aqueous solution adjustment grogs moisture before earthing, makes grogs water content at 25-35%.
7. method according to claim 3, it is characterized in that, described in step (6), nutrient solution prescription comprises following component by mass percentage: brown sugar 1%, and potato is to boil water 5%, lime 3%, magnesium sulfate 0.04%, potassium primary phosphate 0.04%, and surplus is water.
8. method according to claim 7, is characterized in that, described potato is to boil water filters gained for 100g potato slice is added 1000g water after boiling.
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CN107969288A (en) * 2017-12-11 2018-05-01 温立超 A kind of method using Pleurotus eryngii bacteria residue cultivating agaricus bisporus
CN109122036A (en) * 2018-11-07 2019-01-04 广西仁泰生物科技有限公司 A kind of implantation methods of tea tree interplanting agaricus bisporus

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