CN108383648B - Preparation method of mushroom culture medium containing camellia seed shells - Google Patents

Preparation method of mushroom culture medium containing camellia seed shells Download PDF

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Publication number
CN108383648B
CN108383648B CN201810508845.XA CN201810508845A CN108383648B CN 108383648 B CN108383648 B CN 108383648B CN 201810508845 A CN201810508845 A CN 201810508845A CN 108383648 B CN108383648 B CN 108383648B
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Prior art keywords
culture medium
camellia seed
mass
mushrooms
mixture
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CN201810508845.XA
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CN108383648A (en
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杨艳萍
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Guizhou Yishou Agricultural Technology Co ltd
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Guizhou Yishou Agricultural Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention relates to the technical field of edible mushroom culture media, in particular to a mushroom culture medium containing camellia seed hulls and a preparation method thereof.

Description

Preparation method of mushroom culture medium containing camellia seed shells
Technical Field
The invention relates to the technical field of edible fungus culture media, in particular to a mushroom culture medium containing camellia seed shells and a preparation method thereof.
Background
The mushroom belongs to one of edible fungi, the edible part is a mushroom fruiting body, and the mushroom fruiting body is widely applied to Chinese dishes and often exists as a raw material in three vegetarian delicacies.
With the continuous improvement of the demand of people on mushrooms, the development of the mushroom industry is increased, and in the prior art, a large number of researchers are engaged in the research on the cultivation, management and the like of mushrooms, so that the planting cost of the mushrooms is reduced, and the profits of growers are improved; however, technical documents for researching culture media in the mushroom culture process are relatively few, and the method is mainly based on research on the culture media used by the large edible mushrooms, so that the mushrooms are cultured by the culture media of the large edible mushrooms, and the culture media are not targeted, thus causing poor quality and low yield; in view of the above, researchers have also studied the formula of the culture medium adopted by mushrooms, for example, patent application No. 201510944794.1 adopts a mushroom culture medium using rape stalks as main materials and a preparation method thereof, which adopts rape stalks as main materials, so that the carbon source is rich, the raw material source is rich, the cost is low, sufficient nutrients and a good growth environment can be provided for mushrooms, the high yield and the high quality of mushrooms are ensured, the saw dust is soaked in salt water by adopting a nutritional aid for treatment, the carbon source is increased, and 0.79kg of mushrooms are produced per kg of the culture medium; however, due to the unreasonable design of the raw material proportion and unreasonable selected raw materials, the nutrient of the culture medium is insufficient in the process of providing nutrition for the mushrooms, and the quality of the planted mushrooms is poor.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides a mushroom culture medium containing camellia seed shells and a preparation method thereof.
The method is realized by the following technical scheme:
a culture medium for lentinus edodes containing camellia seed husk is prepared from camellia seed husk and rape stem through pulverizing, mixing, adding assistant, stirring, regulating water content to 40-50%, loading in sealed tank, treating at 30-40 deg.C for 3-7 days, adding water, grinding, spraying onto carrier, sterilizing and packing.
Preferably, in the culture medium, the weight of the camellia seed hulls is 3-7 times that of the rape straws, and the addition amount of the auxiliary agent accounts for 1-5% of the weight of the camellia seed hulls. By adopting the proportion, the content of each nutrient in the fresh mushrooms can be improved, the quality of the mushrooms can be improved, and the yield of the mushrooms can be increased.
Preferably, the carrier is a mixture of rice bran and corn paste in a mass ratio of 3: 0.1-0.7. Is helpful for reducing morbidity and improving the yield of the shiitake mushrooms in the culture medium per unit weight.
Preferably, the auxiliary agent is a mixture of chinaberry, potassium dihydrogen phosphate and calcium ascorbate which are mixed according to the mass ratio of 3:1.1-1.7:2.1-4.2, the mixture is ground into powder to obtain mixed powder, aspergillus niger accounting for 1-3% of the mass of the mixed powder is inoculated, and after the mixed powder is stacked for 1-3 days, the mixed powder is dried, crushed and sieved by a 100-mesh and 200-mesh sieve. Improving the quality of the culture medium, increasing the carbon source and the nitrogen source, increasing the nutrient content, promoting the rapid growth of the mushroom and increasing the weight of the single mushroom.
Preferably, the corn paste is prepared by mixing and decocting corn flour and water according to the mass ratio of 1-3: 3-5 to form a sticky paste, adding plant ash accounting for 1-3% of the mass of the corn flour, and uniformly stirring.
By taking the camellia seed hulls as the raw material of the mushroom culture medium, combining with the selection of rape stalks, additives and carriers and processing the preparation conditions of the culture medium, the culture medium can provide sufficient nutrients for mushrooms, improve the quality of the mushrooms, increase the yield of the mushrooms in a unit weight of the culture medium, reduce the cost, and enable the fruiting amount of each kg of the culture medium to reach 0.84-0.86 kg, the content of beta-carotene in the mushrooms to be 75.3-82.1 mg/kg, the content of vitamin B1 to be 0.031-0.041 mg/100g, the content of vitamin B2 to be 0.41-0.47 mg/100g, the content of crude fibers to be 6.5-6.9%, the content of proteins to be 6.9%, and the content of potassium to be 813.1-821.3 mg/100 g.
The detection of the content of the components in the mushroom is to detect the components in the powder of the mushroom after the mushroom is dried and crushed. The specific detection is carried out by the detection methods of beta-carotene, protein, cellulose, potassium, vitamin B1 and vitamin B2 in the prior art.
Detailed Description
The technical solution of the present invention is further defined below with reference to the specific embodiments, but the scope of the claims is not limited to the description.
Example 1
A culture medium for lentinus edodes containing camellia seed husk is prepared from camellia seed husk and rape stem through pulverizing, mixing, adding assistant, stirring, regulating water content to 40%, loading in sealed tank, treating at 30 deg.C for 3 days, adding water, grinding, spraying onto carrier, sterilizing and packing.
The culture medium is characterized in that the mass of the camellia seed hulls is 3 times that of the rape straws, and the addition amount of the auxiliary agent accounts for 1% of the mass of the camellia seed hulls.
The carrier is a mixture of rice bran and corn paste according to a mass ratio of 3: 0.1.
The auxiliary agent is a mixture of chinaberry, potassium dihydrogen phosphate and calcium ascorbate according to the mass ratio of 3:1.1:2.1, grinding the mixture into powder to obtain mixed powder, inoculating aspergillus niger accounting for 1% of the mass of the mixed powder, stacking for 1d, drying, crushing, and sieving with a 100-mesh sieve.
The corn paste is prepared by mixing corn flour and water according to the mass ratio of 1:3, decocting into a viscous state, adding plant ash accounting for 1% of the mass of the corn flour, and uniformly stirring.
Adjusting the water content to be suitable for planting the mushrooms, inoculating mushroom strains to the mushrooms, culturing the mushrooms, drying the cultured mushrooms and the mushrooms, crushing the mushrooms into powder, and detecting the component content of the mushrooms, wherein the specific results are as follows:
0.84kg of shiitake mushrooms are produced per kg of culture medium.
The content of beta-carotene is 75.3mg/kg, the content of vitamin B1 is 0.041mg/100g, the content of vitamin B2 is 0.45mg/100g, the content of crude fiber is 6.7%, the content of protein is 6.5%, and the content of potassium is 821.3mg/100 g.
Example 2
A culture medium for lentinus edodes containing camellia seed husk is prepared from camellia seed husk and rape stem through pulverizing, mixing, adding assistant, stirring, regulating water content to 50%, loading in sealed tank, treating at 40 deg.C for 7 days, adding water, grinding, spraying onto carrier, sterilizing and packing.
The culture medium is characterized in that the mass of the camellia seed hulls is 7 times that of the rape straws, and the addition amount of the auxiliary agent accounts for 5% of the mass of the camellia seed hulls.
The carrier is a mixture of rice bran and corn paste according to a mass ratio of 3: 0.7.
The auxiliary agent is a mixture of chinaberry, potassium dihydrogen phosphate and calcium ascorbate according to the mass ratio of 3:1.7:4.2, grinding the mixture into powder to obtain mixed powder, inoculating aspergillus niger accounting for 3% of the mass of the mixed powder, stacking for 3 days, drying, crushing, and sieving with a 200-mesh sieve.
The corn paste is prepared by mixing corn flour and water according to the mass ratio of 3:5, decocting into a viscous state, adding plant ash accounting for 3% of the mass of the corn flour, and uniformly stirring.
Adjusting the water content to be suitable for planting the mushrooms, inoculating mushroom strains to the mushrooms, culturing the mushrooms, drying the cultured mushrooms and the mushrooms, crushing the mushrooms into powder, and detecting the component content of the mushrooms, wherein the specific results are as follows:
0.86kg of shiitake mushrooms are produced per kg of culture medium.
82.1mg/kg of beta-carotene, 0.041mg/100g of vitamin B1, 0.47mg/100g of vitamin B2, 6.5 percent of crude fiber, 6.9 percent of protein and 813.1mg/100g of potassium.
Example 3
A culture medium for lentinus edodes containing camellia seed husk is prepared from camellia seed husk and rape stem through pulverizing, mixing, adding assistant, stirring, regulating water content to 45%, loading in sealed tank, treating at 35 deg.C for 5 days, adding water, grinding, spraying onto carrier, sterilizing and packing.
The culture medium is characterized in that the mass of the camellia seed hulls is 5 times that of the rape straws, and the addition amount of the auxiliary agent accounts for 3% of the mass of the camellia seed hulls.
The carrier is a mixture of rice bran and corn paste according to a mass ratio of 3: 0.5.
The auxiliary agent is a mixture of chinaberry, potassium dihydrogen phosphate and calcium ascorbate according to the mass ratio of 3:1.5:3.3, grinding the mixture into powder to obtain mixed powder, inoculating aspergillus niger accounting for 2% of the mass of the mixed powder, stacking for 2 days, drying, crushing, and sieving with a 150-mesh sieve.
The corn paste is prepared by mixing corn flour and water according to the mass ratio of 2:3, decocting into a viscous state, adding plant ash accounting for 2% of the mass of the corn flour, and uniformly stirring.
Adjusting the water content to be suitable for planting the mushrooms, inoculating mushroom strains to the mushrooms, culturing the mushrooms, drying the cultured mushrooms and the mushrooms, crushing the mushrooms into powder, and detecting the component content of the mushrooms, wherein the specific results are as follows:
0.85kg of shiitake mushrooms are produced per kg of culture medium.
76.7mg/kg of beta-carotene, 0.039mg/100g of vitamin B1, 0.41mg/100g of vitamin B2, 6.9 percent of crude fiber, 6.5 percent of protein and 816.5mg/100g of potassium.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (1)

1. A preparation method of a mushroom culture medium containing camellia seed hulls is characterized in that the culture medium is prepared by processing camellia seed hulls and rape stalks into powder, mixing, adding an auxiliary agent, stirring uniformly, adjusting the water content to 40-50%, placing the mixture in a sealing groove, controlling the temperature to be 30-40 ℃, treating for 3-7 days, adding water, grinding into slurry, spraying the slurry on a carrier, sterilizing and subpackaging; in the culture medium, the mass of the camellia seed hulls is 3-7 times that of the rape straws, and the addition amount of the auxiliary agent accounts for 1-5% of the mass of the camellia seed hulls; the carrier is a mixture of rice bran and corn paste according to a mass ratio of 3: 0.1-0.7; the corn paste is prepared by mixing and decocting corn flour and water according to the mass ratio of 1-3: 3-5 to form a sticky state, adding plant ash accounting for 1-3% of the mass of the corn flour, and uniformly mixing; the auxiliary agent is a mixture of chinaberry, potassium dihydrogen phosphate and calcium ascorbate according to the mass ratio of 3:1.1-1.7:2.1-4.2, grinding the mixture into powder to obtain mixed powder, inoculating aspergillus niger accounting for 1-3% of the mass of the mixed powder, stacking the mixed powder for 1-3 days, drying, crushing, and sieving with a 100-mesh and 200-mesh sieve.
CN201810508845.XA 2018-05-24 2018-05-24 Preparation method of mushroom culture medium containing camellia seed shells Expired - Fee Related CN108383648B (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102613000A (en) * 2012-04-09 2012-08-01 何寒 Method for cultivating oyster mushrooms at bottoms of tea-oil trees by taking tea-oil tree nutshells as major raw materials
CN103739361A (en) * 2013-12-12 2014-04-23 凤台县千秋食用菌有限公司 Golden mushroom cultivation material containing tea seed shell and preparation method thereof
CN103739357A (en) * 2013-12-12 2014-04-23 颍上县鸿涛菌业专业合作社 Lentinus edodes cultivation material prepared by taking chrysanthemum straws as raw material and preparation method thereof
CN105016853A (en) * 2015-06-26 2015-11-04 何金霞 Needle mushroom medium and method using medium to cultivate needle mushroom
CN107298613A (en) * 2017-07-28 2017-10-27 安徽丰尚生态农业科技有限公司 A kind of high slow release planting material of mushroom high yield and preparation method thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107652039A (en) * 2017-10-11 2018-02-02 广西南宁秀珀生物科技有限公司 Se-enriched bio fertilizer with slow-release function and preparation method thereof
CN107652040A (en) * 2017-10-11 2018-02-02 广西南宁秀珀生物科技有限公司 Bio-fertilizer and preparation method thereof
CN107857630A (en) * 2017-10-11 2018-03-30 广西南宁秀珀生物科技有限公司 Biological organic fertilizer and preparation method thereof
CN107814666A (en) * 2017-12-07 2018-03-20 时科生物科技(上海)有限公司 A kind of charcoal base manure material and its preparation technology beneficial to soil improvement

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102613000A (en) * 2012-04-09 2012-08-01 何寒 Method for cultivating oyster mushrooms at bottoms of tea-oil trees by taking tea-oil tree nutshells as major raw materials
CN103739361A (en) * 2013-12-12 2014-04-23 凤台县千秋食用菌有限公司 Golden mushroom cultivation material containing tea seed shell and preparation method thereof
CN103739357A (en) * 2013-12-12 2014-04-23 颍上县鸿涛菌业专业合作社 Lentinus edodes cultivation material prepared by taking chrysanthemum straws as raw material and preparation method thereof
CN105016853A (en) * 2015-06-26 2015-11-04 何金霞 Needle mushroom medium and method using medium to cultivate needle mushroom
CN107298613A (en) * 2017-07-28 2017-10-27 安徽丰尚生态农业科技有限公司 A kind of high slow release planting material of mushroom high yield and preparation method thereof

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