CN104982469A - Method for controlling strawberry anthracnose - Google Patents
Method for controlling strawberry anthracnose Download PDFInfo
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- CN104982469A CN104982469A CN201510368152.1A CN201510368152A CN104982469A CN 104982469 A CN104982469 A CN 104982469A CN 201510368152 A CN201510368152 A CN 201510368152A CN 104982469 A CN104982469 A CN 104982469A
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Abstract
The invention discloses a method for controlling strawberry anthracnose. The method comprises the following steps: (1) putting Chinese medicinal herbs into water for boiling, and carrying out filtration to obtain a Chinese medicinal herb extract and a filter residue; (2) mixing the filter residue and the Chinese medicinal herb extract with sulfur, copper sulfate, ferrocene aliette, fosetyl-aluminum and soil to obtain culturing soil; (4) planting to-be-planted seedlings in the culture soil for culturing. In the method, the Chinese medicinal herbs comprise mugwort leaves, angelica dahurica, calamus, cortex meliae, rheum officinale, caulis perllae, fructus cnidii, ephedra, urtica cannabina L., cassia twig and pomegranate rind. Through adoption of the method, the strawberry anthracnose can be effectively controlled, and the medicine-resistant (medicine-tolerant) property of strawberry colletotrichum gloeosporioides is relatively low.
Description
Technical field
The present invention relates to strawberry breeding method, particularly, relate to a kind of prevention and controls of Strawberry anthracnose.
Background technology
Strawberry anthracnose is drafted a document caused by certain kind of berries anthrax-bacilus (Colletotrichumfragariae Brooks) infects by fungi Deuteromycotina hair disc spore, and the perfect stage of strawberry anthrax-bacilus is that Ascomycotina small cluster shell belongs to (Glomerella fragariae).Germ survives the winter in incidence tissue or land in invalid body with conidium, can propagate by rainwater and the operation tool carried disease germs, sick leaf, sick fruit etc. by conidium in field meanwhile.
The optimum condition that infects of strawberry anthrax-bacilus is: temperature is 28-32 DEG C, and relative moisture, more than 90%, is typical hot and humid type germ.After late May, when the temperature rises to more than 25 DEG C, strawberry stolon or subaerial tender tissue are subject to infection process; Between the 7-9 month under hot and humid condition, pathogen transmission spreads rapidly, strawberry continuous cropping field particularly continuously after overcast and rainy or shower 2-5 days or typhoon, old residual leaf is many, nitrogenous fertilizer is excessive, young tender and that ventilation and penetrating light the is poor seedling ground morbidity of plant is serious, can cause the destructive loss of strawberry within short-term.In recent years, the generation of this disease was on the rise, especially on strawberry continuous cropping ground, brought serious hindrance to nurturing staff.
The prevention method of the prevention and controls of existing Strawberry anthracnose is all adopt medicament to spray, as Fluoxastrobin (azoxystrobin), kresoxim-methyl (kresoxim-methyl), Difenoconazole (difenoconazole), Enestroburin (enestroburin), Boscalid (boscalid), carbendazim (carbendazim), pyraclostrobin (pyraclostrobin) and Prochloraz (prochloraz) etc.This controlling mode not only effect is poor; Simultaneously multifrequency time, multiple dose are used rear anti-(resistance to) property of medicine easily causing Strawberry anthracnose bacterium to produce higher degree, thus are caused the failure of the control of strawberry subcutaneous ulcer germ.
Summary of the invention
The object of this invention is to provide a kind of prevention and controls of Strawberry anthracnose, the prevention and controls of this Strawberry anthracnose can prevent and treat the generation of Strawberry anthracnose effectively, and what Strawberry anthracnose bacterium produced in addition resists (resistance to) property of medicine degree lower.
To achieve these goals, the invention provides a kind of prevention and controls of Strawberry anthracnose, comprising:
1) Chinese herbal medicine is placed in water to decoct, filter obtained extracts of Chinese herbal medicine and filter residue;
2) root of strawberry shoot is soaked in obtained waiting in extracts of Chinese herbal medicine and carries shoot;
3) by obtained for the mixing of filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene, aliette and soil compost;
4) will wait that carrying shoot plants in compost to cultivate;
Wherein, Chinese herbal medicine is tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum.
By technique scheme, the present invention by utilizing the synergy of the filter residue of Chinese herbal medicine, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene and aliette, and then reaches the generation of the anthracnose fundamentally stopping strawberry.In this kind of prevention and controls, greatly can not only reduce the generation of Strawberry anthracnose, also can reduce anti-(resistance to) property of medicine degree greatly reducing Strawberry anthracnose bacterium and produce simultaneously.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides a kind of prevention and controls of Strawberry anthracnose, comprising:
1) Chinese herbal medicine is placed in water to decoct, filter obtained extracts of Chinese herbal medicine and filter residue;
2) root of strawberry shoot is soaked in obtained waiting in extracts of Chinese herbal medicine and carries shoot;
3) by obtained for the mixing of filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene, aliette and soil compost;
4) will wait that carrying shoot plants in compost to cultivate;
Wherein, Chinese herbal medicine is tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum.
Step 1 in invention) in, the concrete consumption of each raw material can be selected in wide scope, but in order to the effect making the extracts of Chinese herbal medicine that obtains and filter residue can play more excellent control Strawberry anthracnose, preferably, in step 1) in, relative to the tarragon of 100 weight portions, the consumption of the root of Dahurain angelica is 50-70 weight portion, the consumption of calamus is 30-40 weight portion, the consumption of azedarach is 20-50 weight portion, the consumption of rheum officinale is 15-35 weight portion, the consumption of caulis perllae is 10-25 weight portion, the consumption of frutus cnidii is 80-110 weight portion, the consumption of Chinese ephedra is 12-28 weight portion, the consumption of fiber crops leaf nettle is 45-75 weight portion, the consumption of cassia twig is 15-35 weight portion, the consumption of granatum is 60-80 weight portion.
In decoction process of the present invention, the consumption of water also can change in wide scope, but in order to make the active ingredient in Chinese herbal medicine all can extract, preferably, in step 1) in, relative to the Chinese herbal medicine of 100 weight portions, the consumption of water is 1000-5000 weight portion.
Simultaneously, in the present invention, the actual conditions decocted can be selected in wide scope, but all can extract to make the active ingredient in Chinese herbal medicine further, preferably, in step 1) in, decoct and at least meet the following conditions: decoction temperature is 100-120 DEG C, and decocting time is 5-8h.
In addition, in immersion operation of the present invention, in order to make the root of strawberry shoot to absorb fully, preferably, in step 2) in, soak and at least meet the following conditions: soaking temperature is 15-35 DEG C, and soak time is 0.5-1.5h.
On the basis of the above, step 3) in the concrete consumption of each raw material also can change in wide scope, but in order to make the strawberry cultivated, there is more excellent control Strawberry anthracnose ability, preferably, in step 3) in, relative to the soil of 100 weight portions, the consumption of filter residue is 40-80 weight portion, the consumption of extracts of Chinese herbal medicine is 50-70 weight portion, the consumption of sulphur is 8-15 weight portion, the consumption of copper sulphate is 15-20 weight portion, and the consumption of ferrocene is 7-12 weight portion, and the consumption of aliette is 3-5 weight portion.
In order to make step 3 further) in each mixing of materials even, preferably, in step 3) in, mixing at least meets the following conditions: mixing temperature is 15-35 DEG C, and incorporation time is 25-40min.
Below will be described the present invention by embodiment.
Embodiment 1
1) at 110 DEG C, Chinese herbal medicine is placed in water and decocts 6h, filter obtained extracts of Chinese herbal medicine and filter residue; Wherein, the weight ratio of tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum is 100:60:35:35:25:17:100:20:55:25:70, and the ratio of the gross weight of Chinese herbal medicine and the weight of water is 1:30;
2) at 25 DEG C, the root of strawberry shoot is soaked in said herbal medicine extract obtained the waiting of 1h and carry shoot;
3) at 25 DEG C, described filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene, aliette and soil mixing 35min are obtained compost; Wherein, the weight ratio of soil, filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene and aliette is 100:60:60:12:18:9:4;
4) 100 strains are waited carrying shoot plants in above-mentioned compost to cultivate;
5) after Strawberry ripening, infection rate, the diseased fruit rate of the Strawberry anthracnose of statistics strawberry.
Embodiment 2
1) at 100 DEG C, Chinese herbal medicine is placed in water and decocts 5h, filter obtained extracts of Chinese herbal medicine and filter residue; Wherein, the weight ratio of tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum is 100:50:30:20:15:10:80:12:45:15:60, and the ratio of the gross weight of Chinese herbal medicine and the weight of water is 1:10;
2) at 15 DEG C, the root of strawberry shoot is soaked in said herbal medicine extract obtained the waiting of 0.5h and carry shoot;
3) at 15 DEG C, described filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene, aliette and soil mixing 25min are obtained compost; Wherein, the weight ratio of soil, filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene and aliette is 100:40:50:8:15:7:3;
4) 100 strains are waited carrying shoot plants in above-mentioned compost to cultivate;
5) after Strawberry ripening, infection rate, the diseased fruit rate of the Strawberry anthracnose of statistics strawberry.
Embodiment 3
1) at 120 DEG C, Chinese herbal medicine is placed in water and decocts 8h, filter obtained extracts of Chinese herbal medicine and filter residue; Wherein, the weight ratio of tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum is 100:70:40:50:35:25:110:28:75:35:80, and the ratio of the gross weight of Chinese herbal medicine and the weight of water is 1:50;
2) at 35 DEG C, the root of strawberry shoot is soaked in said herbal medicine extract obtained the waiting of 1.5h and carry shoot;
3) at 35 DEG C, described filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene, aliette and soil mixing 40min are obtained compost; Wherein, the weight ratio of soil, filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene and aliette is 100:80:70:15:20:12:5;
4) 100 strains are waited carrying shoot plants in above-mentioned compost to cultivate;
5) after Strawberry ripening, infection rate, the diseased fruit rate of the Strawberry anthracnose of statistics strawberry.
Comparative example 1
Carry out according to the method for embodiment 1, unlike, step 1) middle without tarragon.
Comparative example 2
Carry out according to the method for embodiment 1, unlike, step 1) middle without the root of Dahurain angelica.
Comparative example 3
Carry out according to the method for embodiment 1, unlike, step 1) middle without calamus.
Comparative example 4
Carry out according to the method for embodiment 1, unlike, step 1) middle without azedarach.
Comparative example 5
Carry out according to the method for embodiment 1, unlike, step 1) middle without rheum officinale.
Comparative example 6
Carry out according to the method for embodiment 1, unlike, step 1) middle without caulis perllae.
Comparative example 7
Carry out according to the method for embodiment 1, unlike, step 1) middle without frutus cnidii.
Comparative example 8
Carry out according to the method for embodiment 1, unlike, step 1) middle without Chinese ephedra.
Comparative example 9
Carry out according to the method for embodiment 1, unlike, step 1) in without numb leaf nettle.
Comparative example 10
Carry out according to the method for embodiment 1, unlike, step 1) middle without cassia twig.
Comparative example 11
Carry out according to the method for embodiment 1, unlike, step 1) middle without granatum.
Comparative example 12
Carry out according to the method for embodiment 1, unlike, step 3) middle without filter residue.
Comparative example 13
Carry out according to the method for embodiment 1, unlike, step 3) middle without extracts of Chinese herbal medicine.
Comparative example 14
Carry out according to the method for embodiment 1, unlike, step 3) middle without sulphur.
Comparative example 15
Carry out according to the method for embodiment 1, unlike, step 3) middle without copper sulphate.
Comparative example 16
Carry out according to the method for embodiment 1, unlike, step 3) middle without ferrocene.
Comparative example 17
Carry out according to the method for embodiment 1, unlike, step 3) middle without aliette.
Comparative example 18
Carry out according to the method for embodiment 1, unlike, without step 2).
Comparative example 19
Plant in soil by strawberry shoot, every two weeks sprays and once spills Fluoxastrobin solution, after Strawberry ripening, and infection rate, the diseased fruit rate of the Strawberry anthracnose of statistics strawberry.
Comparative example 20
Carry out according to the method for embodiment 1, unlike, without step 1) and 2), and step 3) middle without filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene, aliette; After Strawberry ripening, infection rate, the diseased fruit rate of the Strawberry anthracnose of statistics strawberry.
Table 1
| Infection rate/the % of Strawberry anthracnose | Diseased fruit rate/% | |
| Embodiment 1 | 1 | 3 |
| Embodiment 2 | 2 | 5 |
| Embodiment 3 | 1 | 4 |
| Comparative example 1 | 15 | 20 |
| Comparative example 2 | 13 | 15 |
| Comparative example 3 | 8 | 17 |
| Comparative example 4 | 17 | 18 |
| Comparative example 5 | 16 | 19 |
| Comparative example 6 | 15 | 13 |
| Comparative example 7 | 19 | 8 |
| Comparative example 8 | 11 | 7 |
| Comparative example 9 | 12 | 17 |
| Comparative example 10 | 15 | 18 |
| Comparative example 11 | 16 | 12 |
| Comparative example 12 | 9 | 11 |
| Comparative example 13 | 21 | 10 |
| Comparative example 14 | 18 | 13 |
| Comparative example 15 | 16 | 14 |
| Comparative example 16 | 20 | 12 |
| Comparative example 17 | 17 | 15 |
| Comparative example 18 | 19 | 14 |
| Comparative example 19 | 9 | 10 |
| Comparative example 20 | 65 | 85 |
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible combination.
In addition, also can be combined between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (7)
1. a prevention and controls for Strawberry anthracnose, is characterized in that, comprising:
1) Chinese herbal medicine is placed in water to decoct, filter obtained extracts of Chinese herbal medicine and filter residue;
2) root of strawberry shoot is soaked in obtained waiting in described extracts of Chinese herbal medicine and carries shoot;
3) by obtained for the mixing of described filter residue, extracts of Chinese herbal medicine, sulphur, copper sulphate, ferrocene, aliette and soil compost;
4) wait that carrying shoot plants in described compost to cultivate by described;
Wherein, described Chinese herbal medicine is tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum.
2. prevention and controls according to claim 1, it is characterized in that, in step 1) in, relative to the tarragon of 100 weight portions, the consumption of the described root of Dahurain angelica is 50-70 weight portion, the consumption of described calamus is 30-40 weight portion, the consumption of described azedarach is 20-50 weight portion, the consumption of described rheum officinale is 15-35 weight portion, the consumption of described caulis perllae is 10-25 weight portion, the consumption of described frutus cnidii is 80-110 weight portion, the consumption of described Chinese ephedra is 12-28 weight portion, the consumption of described numb leaf nettle is 45-75 weight portion, the consumption of described cassia twig is 15-35 weight portion, the consumption of described granatum is 60-80 weight portion.
3. prevention and controls according to claim 1, is characterized in that, in step 1) in, relative to the Chinese herbal medicine of 100 weight portions, the consumption of described water is 1000-5000 weight portion.
4. prevention and controls according to claim 1, is characterized in that, in step 1) in, described decoction at least meets the following conditions: decoction temperature is 100-120 DEG C, and decocting time is 5-8h.
5. according to the prevention and controls in claim 1-4 described in any one, it is characterized in that, in step 2) in, described immersion at least meets the following conditions: soaking temperature is 15-35 DEG C, and soak time is 0.5-1.5h.
6. according to the prevention and controls in claim 1-4 described in any one, it is characterized in that, in step 3) in, relative to the soil of 100 weight portions, the consumption of described filter residue is 40-80 weight portion, and the consumption of described extracts of Chinese herbal medicine is 50-70 weight portion, the consumption of described sulphur is 8-15 weight portion, the consumption of described copper sulphate is 15-20 weight portion, and the consumption of described ferrocene is 7-12 weight portion, and the consumption of described aliette is 3-5 weight portion.
7. prevention and controls according to claim 6, is characterized in that, in step 3) in, described mixing at least meets the following conditions: mixing temperature is 15-35 DEG C, and incorporation time is 25-40min.
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| CN105493997A (en) * | 2015-12-04 | 2016-04-20 | 安徽省康之源农业科技有限公司 | Nuisanceless strawberry anthracnose prevention and treatment method |
| CN106614820A (en) * | 2016-11-24 | 2017-05-10 | 蚌埠市宗洼草鸡养殖农民专业合作社 | Composition for preventing and treating anthracnose of spinach |
| CN107251907A (en) * | 2017-08-01 | 2017-10-17 | 河南柏裕植物免疫科技有限公司 | Jujube anthrax vaccines |
| CN108157393A (en) * | 2017-12-13 | 2018-06-15 | 中央民族大学 | A kind of Angelica Dahurica extract and its application as Genes For Plant Tolerance fungus |
| CN113598201A (en) * | 2021-08-02 | 2021-11-05 | 海南大学 | Botanical biocontrol bacterium preparation for preventing and treating rubber anthrax |
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Application publication date: 20151021 |