CN105028519A - Bacteriostatic agent for prevention and treatment of strawberry anthracnose and preparation method and application thereof - Google Patents
Bacteriostatic agent for prevention and treatment of strawberry anthracnose and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a bacteriostatic agent for prevention and treatment of strawberry anthracnose and a preparation method and application thereof, and the method comprises the following steps: 1) putting Chinese herbal medicines into water for decoction, and then filtering the decocted Chinese herbal medicines to obtain Chinese herbal extract; 2) mixing the Chinese herbal extract with sulphur, copper sulfate, ferrocene and aliette to obtain the bacteriostatic agent for prevention and treatment of the strawberry anthracnose; the Chinese herbal medicines comprise folium artemisiae argyi, radix angelicae dahuricae, acorus calamus, azedarach, rheum officinale, caulis perillae, fructus cnidii, ephedra, urtica cannabina L., cassia twig and pomegranate bark. The bacteriostatic agent prepared by the method has an excellent curative effect on the strawberry anthracnose, the residual quantity is low in strawberries, drug resistance (tolerance) generated by the strawberry anthracnose after applying the bacteriostatic agent is lower; furthermore, the method and steps for preparing the bacteriostatic agent are simple, and the raw materials are easy to obtain.
Description
Technical field
The present invention relates to bacteriostatic agent, particularly, relate to a kind of bacteriostatic agent preventing and treating Strawberry anthracnose and its preparation method and application.
Background technology
Strawberry anthracnose is drafted a document caused by certain kind of berries anthrax-bacilus (ColletotrichumfragariaeBrooks) infects by fungi Deuteromycotina hair disc spore, and the perfect stage of strawberry anthrax-bacilus is that Ascomycotina small cluster shell belongs to (Glomerellafragariae).Germ survives the winter in incidence tissue or land in invalid body with conidium, can propagate by rainwater and the operation tool carried disease germs, sick leaf, sick fruit etc. by conidium in field meanwhile.
The optimum condition that infects of strawberry anthrax-bacilus is: temperature is 28-32 DEG C, and relative moisture, more than 90%, is typical hot and humid type germ.After late May, when the temperature rises to more than 25 DEG C, strawberry stolon or subaerial tender tissue are subject to infection process; Between the 7-9 month under hot and humid condition, pathogen transmission spreads rapidly, strawberry continuous cropping field particularly continuously after overcast and rainy or shower 2-5 days or typhoon, old residual leaf is many, nitrogenous fertilizer is excessive, young tender and that ventilation and penetrating light the is poor seedling ground morbidity of plant is serious, can cause the destructive loss of strawberry within short-term.In recent years, the generation of this disease was on the rise, especially on strawberry continuous cropping ground, brought serious hindrance to nurturing staff.
The chemical agent of existing treatment Strawberry anthracnose has Fluoxastrobin (azoxystrobin), kresoxim-methyl (kresoxim-methyl), Difenoconazole (difenoconazole), Enestroburin (enestroburin), Boscalid (boscalid), carbendazim (carbendazim), pyraclostrobin (pyraclostrobin) and Prochloraz (prochloraz) etc.In the process of these antibacterial agents control strawberry subcutaneous ulcer germ, there is following defect: 1) multifrequency time, multiple dose are used rear anti-(resistance to) property of medicine easily causing Strawberry anthracnose bacterium to produce higher degree, thus caused the failure of the control of strawberry subcutaneous ulcer germ.2) residual quantity of strawberry Pesticides is comparatively large, greatly hinders the raising of the quality of strawberry.
Summary of the invention
The object of this invention is to provide a kind of bacteriostatic agent preventing and treating Strawberry anthracnose and its preparation method and application, the bacteriostatic agent obtained by the method for Strawberry anthracnose, there is excellent curative effect and in strawberry residual quantity low, and anti-(resistance to) property of medicine degree that Strawberry anthracnose produces after using this bacteriostatic agent is lower; In addition, the method step preparing this antibacterial agent is simple, and raw material is easy to get.
To achieve these goals, the invention provides a kind of preparation method preventing and treating the bacteriostatic agent of Strawberry anthracnose, comprising:
1) Chinese herbal medicine is placed in water to decoct, filter obtained extracts of Chinese herbal medicine;
2) extracts of Chinese herbal medicine is mixed the obtained bacteriostatic agent preventing and treating Strawberry anthracnose with sulphur, copper sulphate, ferrocene and aliette;
Wherein, Chinese herbal medicine is tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum.
Present invention also offers a kind of bacteriostatic agent preventing and treating Strawberry anthracnose, bacteriostatic agent is obtained by the preparation of above-mentioned method.
Invention further provides the application of a kind of bacteriostatic agent according to above-mentioned control Strawberry anthracnose in the process of control Strawberry anthracnose.
Pass through technique scheme, the present invention is by utilizing tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig, granatum, sulphur, copper sulphate, synergy between ferrocene and aliette, this bacteriostatic agent is made not only to have the ability of excellent control Strawberry anthracnose, even if simultaneously this bacteriostatic agent repeatedly, a large amount of use after Strawberry anthracnose also can not be caused to produce anti-(resistance to) property of medicine of higher degree, and in the strawberry fruit of maturation, the content of each component of bacteriostatic agent is all lower.In addition, the method step preparing this antibacterial agent is simple, and raw material is easy to get, and significantly reduces the cost producing bacteriostatic agent.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides a kind of preparation method preventing and treating the bacteriostatic agent of Strawberry anthracnose, comprising:
1) Chinese herbal medicine is placed in water to decoct, filter obtained extracts of Chinese herbal medicine;
2) extracts of Chinese herbal medicine is mixed the obtained bacteriostatic agent preventing and treating Strawberry anthracnose with sulphur, copper sulphate, ferrocene and aliette;
Wherein, Chinese herbal medicine is tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum.
In step 1 of the present invention) in, the consumption of each raw material can be selected in wide scope, but can farthest play to make the synergy between each material, preferably, in step 1) in, relative to the tarragon of 100 weight portions, the consumption of the root of Dahurain angelica is 50-70 weight portion, the consumption of calamus is 30-40 weight portion, the consumption of azedarach is 20-50 weight portion, the consumption of rheum officinale is 15-35 weight portion, the consumption of caulis perllae is 10-25 weight portion, the consumption of frutus cnidii is 80-110 weight portion, the consumption of Chinese ephedra is 12-28 weight portion, the consumption of fiber crops leaf nettle is 45-75 weight portion, the consumption of cassia twig is 15-35 weight portion, the consumption of granatum is 60-80 weight portion.
Simultaneously, step 1 above-mentioned) in, the consumption of water also can produce certain impact to the effect of obtained bacteriostatic agent, in order to medium-height grass the effective elements of the medicine being made to be extracted fully in the process decocted, preferably, in step 1) in, relative to the Chinese herbal medicine of 100 weight portions, the consumption of water is 1000-5000 weight portion.
In addition, the actual conditions decocted in invention can be selected in wide scope, especially the temperature and time of boiling, but in order to extract the active ingredient in Chinese herbal medicine further, preferably, in step 1) in, decoct and at least meet the following conditions: decoction temperature is 100-120 DEG C, and decocting time is 5-8h.
In step 2 of the present invention) in, the concrete consumption of each component can change equally in wide scope, but in order to give full play to extracts of Chinese herbal medicine and sulphur, copper sulphate, synergy between ferrocene and aliette to reach the fungistatic effect improving bacteriostatic agent, preferably, in step 2) in, relative to the extracts of Chinese herbal medicine of 100 weight portions, the consumption of sulphur is 4-7 weight portion, the consumption of copper sulphate is 10-15 weight portion, the consumption of ferrocene is 3-9 weight portion, and the consumption of aliette is 8-15 weight portion.
On the basis of the above, step 2) in mixing actual conditions also can change in wide scope, but can mix fully to make each component, preferably, in step 2) in, mixing at least meets the following conditions: mixing temperature is 15-35 DEG C, and incorporation time is 25-40min.
Present invention also offers a kind of bacteriostatic agent preventing and treating Strawberry anthracnose, bacteriostatic agent is obtained by the preparation of above-mentioned method.
Invention further provides the application of a kind of bacteriostatic agent according to above-mentioned control Strawberry anthracnose in the process of control Strawberry anthracnose.
Below will be described the present invention by embodiment.In following examples, the Fluoxastrobin in strawberry, copper sulphate, ferrocene and aliette residual quantity are recorded by high performance liquid chromatography.
Embodiment 1
1) tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum decocted in water at 110 DEG C 6h, filter obtained extracts of Chinese herbal medicine; Wherein, the weight ratio of tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum is 100:60:35:35:25:17:85:20:60:20:70, and the ratio of the gross weight of Chinese herbal medicine and the weight of water is 1:30;
2) at 25 DEG C, extracts of Chinese herbal medicine is obtained with sulphur, copper sulphate, ferrocene and aliette mixing 30min the bacteriostatic agent A1 preventing and treating Strawberry anthracnose, wherein, the weight ratio of extracts of Chinese herbal medicine and sulphur, copper sulphate, ferrocene and aliette is 100:6:13:6:10.
Embodiment 2
1) tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum decocted in water at 100 DEG C 5h, filter obtained extracts of Chinese herbal medicine; Wherein, the weight ratio of tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum is 100:50:30:20:15:10:80:12:45:15:60, and the ratio of the gross weight of Chinese herbal medicine and the weight of water is 1:10;
2) at 15 DEG C, extracts of Chinese herbal medicine is obtained with sulphur, copper sulphate, ferrocene and aliette mixing 25min the bacteriostatic agent A2 preventing and treating Strawberry anthracnose, wherein, the weight ratio of extracts of Chinese herbal medicine and sulphur, copper sulphate, ferrocene and aliette is 100:4:10:3:8.
Embodiment 3
1) tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum decocted in water at 120 DEG C 8h, filter obtained extracts of Chinese herbal medicine; Wherein, the weight ratio of tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum is 100:70:40:50:35:25:110:28:75:35:80, and the ratio of the gross weight of Chinese herbal medicine and the weight of water is 1:50;
2) at 35 DEG C, extracts of Chinese herbal medicine is obtained with sulphur, copper sulphate, ferrocene and aliette mixing 40min the bacteriostatic agent A3 preventing and treating Strawberry anthracnose, wherein, the weight ratio of extracts of Chinese herbal medicine and sulphur, copper sulphate, ferrocene and aliette is 100:7:15:9:15.
Comparative example 1
Obtained bacteriostatic agent B1 is carried out according to the method for embodiment 1, unlike, step 1) middle without tarragon.
Comparative example 2
Obtained bacteriostatic agent B2 is carried out according to the method for embodiment 1, unlike, step 1) middle without the root of Dahurain angelica.
Comparative example 3
Obtained bacteriostatic agent B3 is carried out according to the method for embodiment 1, unlike, step 1) middle without calamus.
Comparative example 4
Obtained bacteriostatic agent B4 is carried out according to the method for embodiment 1, unlike, step 1) middle without azedarach.
Comparative example 5
Obtained bacteriostatic agent B5 is carried out according to the method for embodiment 1, unlike, step 1) middle without rheum officinale.
Comparative example 6
Obtained bacteriostatic agent B6 is carried out according to the method for embodiment 1, unlike, step 1) middle without caulis perllae.
Comparative example 7
Obtained bacteriostatic agent B7 is carried out according to the method for embodiment 1, unlike, step 1) middle without frutus cnidii.
Comparative example 8
Obtained bacteriostatic agent B8 is carried out according to the method for embodiment 1, unlike, step 1) middle without Chinese ephedra.
Comparative example 9
Carry out obtained bacteriostatic agent B9 according to the method for embodiment 1, unlike, step 1) in without numb leaf nettle.
Comparative example 10
Obtained bacteriostatic agent B10 is carried out according to the method for embodiment 1, unlike, step 1) middle without cassia twig.
Comparative example 11
Obtained bacteriostatic agent B11 is carried out according to the method for embodiment 1, unlike, step 1) middle without granatum.
Comparative example 12
Obtained bacteriostatic agent B12 is carried out according to the method for embodiment 1, unlike, step 2) middle without sulphur.
Comparative example 13
Obtained bacteriostatic agent B13 is carried out according to the method for embodiment 1, unlike, step 2) middle without copper sulphate.
Comparative example 14
Obtained bacteriostatic agent B14 is carried out according to the method for embodiment 1, unlike, step 2) middle without ferrocene.
Comparative example 15
Obtained bacteriostatic agent B15 is carried out according to the method for embodiment 1, unlike, step 2) middle without aliette.
Test example 1
Get a collection of strawberry shoot, then being undertaken infecting by Strawberry anthracnose bacterium makes strawberry shoot suffer from Strawberry anthracnose, then above-mentioned strawberry shoot is divided into 20 groups, often organizes 50 strains, wherein 18 groups are sprayed with above-mentioned bacteriostatic agent A1-A3 and B1-B15 respectively, every 12h sprays once, 19th group to spray water, the 20th group is sprayed Fluoxastrobin solution, by observing the cure rate adding up strawberry after one week, wherein
concrete outcome is in table 1.
Test example 2
After Strawberry ripening, record Fluoxastrobin in strawberry, copper sulphate, ferrocene and aliette residual quantity by high performance liquid chromatography.Concrete outcome is in table 1.
Table 1
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible combination.
In addition, also can be combined between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (8)
1. prevent and treat a preparation method for the bacteriostatic agent of Strawberry anthracnose, it is characterized in that, comprising:
1) Chinese herbal medicine is placed in water to decoct, filter obtained extracts of Chinese herbal medicine;
2) described extracts of Chinese herbal medicine is mixed the obtained bacteriostatic agent preventing and treating Strawberry anthracnose with sulphur, copper sulphate, ferrocene and aliette;
Wherein, described Chinese herbal medicine is tarragon, the root of Dahurain angelica, calamus, azedarach, rheum officinale, caulis perllae, frutus cnidii, Chinese ephedra, numb leaf nettle, cassia twig and granatum.
2. preparation method according to claim 1, wherein, in step 1) in, relative to the tarragon of 100 weight portions, the consumption of the described root of Dahurain angelica is 50-70 weight portion, the consumption of described calamus is 30-40 weight portion, the consumption of described azedarach is 20-50 weight portion, the consumption of described rheum officinale is 15-35 weight portion, the consumption of described caulis perllae is 10-25 weight portion, the consumption of described frutus cnidii is 80-110 weight portion, the consumption of described Chinese ephedra is 12-28 weight portion, the consumption of described numb leaf nettle is 45-75 weight portion, the consumption of described cassia twig is 15-35 weight portion, the consumption of described granatum is 60-80 weight portion.
3. preparation method according to claim 1, wherein, in step 1) in, relative to the Chinese herbal medicine of 100 weight portions, the consumption of described water is 1000-5000 weight portion.
4. preparation method according to claim 1, wherein, in step 1) in, described decoction at least meets the following conditions: decoction temperature is 100-120 DEG C, and decocting time is 5-8h.
5. according to the preparation method in claim 1-4 described in any one, wherein, in step 2) in, relative to the described extracts of Chinese herbal medicine of 100 weight portions, the consumption of described sulphur is 4-7 weight portion, the consumption of described copper sulphate is 10-15 weight portion, and the consumption of described ferrocene is 3-9 weight portion, and the consumption of described aliette is 8-15 weight portion.
6. according to the preparation method in claim 1-4 described in any one, wherein, in step 2) in, described mixing at least meets the following conditions: mixing temperature is 15-35 DEG C, and incorporation time is 25-40min.
7. prevent and treat a bacteriostatic agent for Strawberry anthracnose, it is characterized in that, described bacteriostatic agent is obtained by the method preparation in claim 1-6 described in any one.
8. the application of the bacteriostatic agent of a control Strawberry anthracnose according to claim 7 in the process of control Strawberry anthracnose.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070373A (en) * | 2016-06-02 | 2016-11-09 | 蚌埠市燕山草莓专业合作社 | A kind of Strawberry seedling anthrax control agent |
| CN107897215A (en) * | 2017-11-27 | 2018-04-13 | 桂林斯泰硕农农业科技有限公司 | Method for preventing and treating leaf blight disease of Bufosna |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047919A (en) * | 2009-11-10 | 2011-05-11 | 刘太玉 | Plant medicament for preventing and controlling rice bacterial leaf blight |
| CN103404541A (en) * | 2013-06-28 | 2013-11-27 | 句容市白兔镇沁香果品专业合作社 | Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide |
| CN104322577A (en) * | 2014-11-27 | 2015-02-04 | 周可幸 | Composite germicide |
| CN104336115A (en) * | 2013-08-09 | 2015-02-11 | 天津市丰收果树种植专业合作社 | Preventing and treating medicament for bacterial black rot of walnuts and preparation method of preventing and treating medicament |
-
2015
- 2015-06-26 CN CN201510366491.6A patent/CN105028519A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047919A (en) * | 2009-11-10 | 2011-05-11 | 刘太玉 | Plant medicament for preventing and controlling rice bacterial leaf blight |
| CN103404541A (en) * | 2013-06-28 | 2013-11-27 | 句容市白兔镇沁香果品专业合作社 | Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide |
| CN104336115A (en) * | 2013-08-09 | 2015-02-11 | 天津市丰收果树种植专业合作社 | Preventing and treating medicament for bacterial black rot of walnuts and preparation method of preventing and treating medicament |
| CN104322577A (en) * | 2014-11-27 | 2015-02-04 | 周可幸 | Composite germicide |
Non-Patent Citations (6)
| Title |
|---|
| 余本水: "《高毒农药替代品应用技术指南》", 31 October 2008, 中国农业出版社 * |
| 周成勇: "《化学综合实验》", 30 November 2011, 中国石化出版社 * |
| 石明旺等: "《新编常用农药安全使用指南》", 28 February 2011, 化学工业出版社 * |
| 程珍等: "麻叶荨麻籽抑菌成分和抑菌特性的研究", 《生物技术》 * |
| 薛广波: "《现代消毒学》", 31 July 2002, 人民军医出版社 * |
| 靳士英: "《新编中医学》", 30 November 1997, 人民军医出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070373A (en) * | 2016-06-02 | 2016-11-09 | 蚌埠市燕山草莓专业合作社 | A kind of Strawberry seedling anthrax control agent |
| CN107897215A (en) * | 2017-11-27 | 2018-04-13 | 桂林斯泰硕农农业科技有限公司 | Method for preventing and treating leaf blight disease of Bufosna |
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Application publication date: 20151111 |